Journal articles on the topic 'Histomorphometric'

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1

Désévaux, C., B. Martineau-Doizé, and S. Laverty. "Comparison of Histomorphometric Measurements of Equine Sternal Cancellous Bone with Bone from other Skeletal Sites." Veterinary and Comparative Orthopaedics and Traumatology 11, no. 03 (1998): 158–63. http://dx.doi.org/10.1055/s-0038-1632539.

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SummaryHistomorphometric analysis of bone biopsy specimens in humans is a reliable techniqu e which provides information on bone status and turnover. A techniqu e of sternal biopsy has been recently developed in “standin g horses” . The purpose of the present study was to demonstrate that the histomorphometric analysis of sternal cancellous bone was representative of other skeletal sites in horses.Post-mortem cancellous bone samples were obtained from the sternum, 12th rib, ilium, 1st lumbar vertebra and the tibia, of seven adult horses and embedded in methylmethacrylate. Sections, 7 μm thick, were cut and were stained with Goldner’s green trichrome. Histomorphometric parameters were measured by a semi-automated method. Sections from the sternum were qualitativel y similar to those from the rib, ilium and vertebra. Histomorphometric measurements were easily performed on the sternal, ilium, rib and vertebral sections. The tibial sections were not suitable for evaluation. The sternal histomorphometric parameters of bone formation and resorption were overall representative of those from other skeletal sites.The present study demonstrated that sternal cancellous bone is acceptable for bone histomorphometry in horses as it reflects overall skeletal osseous activity.This study compares qualitatively and quantitatively cancellous bone from the sternum, 12th rib, ilium, first lumbar vertebra and the tibia of seven adult horses. Sternal cancellous bone is acceptable for bone histomorphometry and reflects overall skeletal osseous activity.
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Kulak, Carolina A. Moreira, and David W. Dempster. "Bone histomorphometry: a concise review for endocrinologists and clinicians." Arquivos Brasileiros de Endocrinologia & Metabologia 54, no. 2 (March 2010): 87–98. http://dx.doi.org/10.1590/s0004-27302010000200002.

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Bone histomorphometry is a quantitative histological examination of an undecalcified bone biopsy performed to obtain quantitative information on bone remodeling and structure. Labeling agents taken before the procedure deposit at sites of bone formation allowing a dynamic analysis. Biopsy is indicated to make the diagnosis of subclinical osteomalacia, to characterize the different forms of renal osteodystrophy and to elucidate cases of unexplained skeletal fragility. Bone histomorphometric parameters are divided into structural and remodeling subgroups, with the latter being subdivided into static and dynamic categories. Metabolic bone disorders such as osteomalacia, hyperparathyroidism, hypothyroidism, osteoporosis and renal osteodystrophy display different histomorphometric profiles. Antiresorptive and anabolic drugs used for the treatment of osteoporosis also induce characteristic changes in the bone biopsy. Bone histomorphometry is an important research tool in the field of bone metabolism and provides information that is not available by any other investigative approach.
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Karydi, Christina, Julieta Gómez García-Donas, Konstantina Tsiminikaki, Andrea Bonicelli, Konstantinos Moraitis, and Elena F. Kranioti. "Estimation of Age-at-Death Using Cortical Bone Histomorphometry of the Rib and Femur: A Validation Study on a British Population." Biology 11, no. 11 (November 4, 2022): 1615. http://dx.doi.org/10.3390/biology11111615.

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Histomorphometry constitutes a valuable tool for age estimation. Histological interpopulation variability has been shown to affect the accuracy of age estimation techniques and therefore validation studies are required to test the accuracy of the pre-existing methodologies. The present research constitutes a validation study of widely known histological methods on the sixth rib and the femoral midshaft of a 19th century British population originating from Blackburn, England. An evaluation of the histomorphometric features of eleven ribs and five femora was performed and used to test the accuracy of selected methods. Results indicated that osteon area and circularity were the only histomorphometric variables that presented significant interpopulation variability. Cho et al.’s method for the ribs and the average value produced using Kerley and Ubelaker’s method for intact osteon and percentage of lamellar bone equations for femur were considered the only reliable markers for estimating the age on the Blackburn sample. In the case of old individuals, Goliath et al.’s method provided more satisfactory results. Overall, the present study provides evidence on the applicability of the aging histomorphometric methods on a British sample and highlights the limitations of applying histomorphometric methods developed on different reference populations than the one under investigation.
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Aissanou, Sofiane, and Abdelhanine Ayad. "Histomorphometric changes of testicular tissues by season and age of Algerian local donkeys (Equus asinus)." Acta Scientiarum. Animal Sciences 44 (June 30, 2022): e55775. http://dx.doi.org/10.4025/actascianimsci.v44i1.55775.

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The present investigation was undertaken to highlight the histomorphometric changes of testicular tissues according to season and age in donkeys (Equus asinus) under northern Algeria conditions. The experiment was conducted from February 2019 to January 2020. A total of 21 sexually mature donkeys were selected randomly. The testis were immediately collected after slaughter for the histological observation. The analysis of the correlation coefficients shows negative correlations between DL and other parameters such as DST, GCEH, TTA and ITSA, ranged between -0.24 and -0.79. Also, there were a high negative correlations between season and the histomorphometric parameters, except DL was revealed positive (r=0.65). On the other hand, there were positive correlations between age and testicular histomorphometrics parameters. The results indicated that DST, GCEH, TTA and ITSA values were significantly higher in winter and autumn seasons than in spring and summer seasons. It is also noteworthy that DST, GCEH, TTA and ITSA values were significantly higher in adult and aged donkeys than in young donkeys. Our comparative analysis of histological parameters, suggests that the sexual activity usually occurs during winter and autumn in local donkeys of Algeria. In addition, our results of histomorphometric of testicular tissues are correlated with age of donkeys.
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Mohan, Suresh, Iván Coto Hernández, Martin K. Selig, Shinsuke Shibata, and Nate Jowett. "Stain-Free Resolution of Unmyelinated Axons in Transgenic Mice Using Fluorescence Microscopy." Journal of Neuropathology & Experimental Neurology 78, no. 12 (September 23, 2019): 1178–80. http://dx.doi.org/10.1093/jnen/nlz099.

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Abstract Though unmyelinated fibers predominate axon counts within peripheral nerves, they are frequently excluded in histomorphometric assessment as they cannot be readily resolved by light microscopy. Herein, we demonstrate stain-free resolution of unmyelinated axons in Sox10-Venus mice by widefield fluorescence imaging of sciatic nerve cryosections. Optional staining of cryosections using a rapid and nontoxic myelin-specific dye (FluoroMyelin Red) enables robust synchronous resolution of myelinated and unmyelinated fibers, comprising a high-throughput platform for neural histomorphometry.
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De Maré, Annelies, Anja Verhulst, Etienne Cavalier, Pierre Delanaye, Geert J. Behets, Bjorn Meijers, Dirk Kuypers, Patrick C. D’Haese, and Pieter Evenepoel. "Clinical Inference of Serum and Bone Sclerostin Levels in Patients with End-Stage Kidney Disease." Journal of Clinical Medicine 8, no. 12 (November 20, 2019): 2027. http://dx.doi.org/10.3390/jcm8122027.

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Mounting evidence indicates that sclerostin, a well-known inhibitor of bone formation, may qualify as a clinically relevant biomarker of chronic kidney disease-related mineral and bone disorder (CKD-MBD), including abnormal mineral and bone metabolism and extraskeletal calcification. For this purpose, in this study we investigate the extent to which circulating sclerostin, skeletal sclerostin expression, bone histomorphometric parameters, and serum markers of bone metabolism associate with each other. Bone biopsies and serum samples were collected in a cohort of 68 end-stage kidney disease (ESKD) patients. Serum sclerostin levels were measured using 4 different commercially available assays. Skeletal sclerostin expression was evaluated on immunohistochemically stained bone sections. Quantitative bone histomorphometry was performed on Goldner stained tissue sections. Different serum markers of bone metabolism were analyzed using in-house techniques or commercially available assays. Despite large inter-assay differences for circulating sclerostin, results obtained with the 4 assays under study closely correlated with each other, whilst moderate significant correlations with skeletal sclerostin expression were also found. Both skeletal and circulating sclerostin negatively correlated with histomorphometric bone and serum parameters reflecting bone formation and turnover. In this study, the unique combined evaluation of bone sclerostin expression, bone histomorphometry, bone biomarkers, and serum sclerostin levels, as assessed by 4 different assays, demonstrated that sclerostin may qualify as a clinically relevant marker of disturbed bone metabolism in ESKD patients.
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Maggio, Ariane, and Daniel Franklin. "Femoral histomorphometric age-at-death studies: The issue of sample size and standard error." Medicine, Science and the Law 60, no. 4 (August 5, 2020): 257–65. http://dx.doi.org/10.1177/0025802420945939.

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Extant histomorphometric aging methods based on the analysis of the femoral cortex generally report small samples ( N<100) and highly variable standard error of the estimate (SEE) values (±1.51‒16.98 years). The present paper reviews the published literature on femoral histomorphometry for age-at-death estimation in order to examine the relationship between sample size and SEE values, and makes recommendations for minimum reporting requirements for age-at-death studies based on statistical data. The SEE from a total of 33 studies are analysed. Sample size and confidence intervals are explored using Hennig and Cooper’s simulation modelling. Analysis of effect size through a fixed-effect model is performed on 5/33 studies to examine the relationship between sample size and effect size. The pooled sex formulae from Nor et al., Martrille et al. and Thompson and the two sex-specific formulae of Pfeiffer are examined, as they report mean and standard deviation values for both chronological and estimated ages. The results of these analyses support sampling theory, specifically wide variation in SEE when N<100, narrowing as the sample size increases, and lower effect sizes in the larger of the five studies examined. The findings provide some support for a minimum threshold of 100‒150 individuals for histomorphometric age-at-death estimation. Analysis of effect size is suggested for future investigation in meta-analyses of forensic anthropological age-estimation studies. To ensure increased precision and meaningful comparison, large samples should be used for histomorphometry, and authors should report SEE and discrete statistics (e.g. n, mean, standard deviation) for both chronological age and estimated age.
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Boivin, Georges, Pascale Chavassieux, Marie-Claire Chapuy, Pierre J. Meunier, and Charles A. Baud. "Skeletal fluorosis: Histomorphometric findings." Journal of Bone and Mineral Research 5, S1 (February 25, 2010): S185—S189. http://dx.doi.org/10.1002/jbmr.5650051382.

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9

MIRANDA, Maria Angelina Carvalho, Álvaro Antônio Bandeira FERRAZ, Ana Lúcia Coutinho DOMINGUES, Renata Carvalho Miranda CHAVES, Norma JUCÁ, and Diógenes Luiz da MOTA. "IMPROVEMENT OF SCHISTOSOMAL PORTAL HYPERTENSIVE COLOPATHY AFTER SURGICAL TREATMENT." Arquivos de Gastroenterologia 50, no. 2 (April 2013): 153–56. http://dx.doi.org/10.1590/s0004-28032013000200026.

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Context Data on vascular alterations in patients with hepatosplenic schistosomiasis and portal hypertensive colopathy and changes in these after surgery to decrease portal hypertension are limited. Objective The purpose of this study was to analyse the alterations of portal hypertensive colopathy previously and 6-12 months after splenectomy and gastric devascularization. Methods Twelve patients with hepatosplenic schistosomiasis who also had upper gastrointestinal bleeding were studied prospectively. Their endoscopic findings before and 6-12 months after the surgery were analysed. In addition, mucosal biopsies from ascending colon, sigmoid colon and rectum at these time points were subjected to histological and histomorphometric assessment. It was used a control group due to lack of normal pattern of the histomorphometric measures of vessels in individuals without portal hypertension. The critical level of significance adopted in all tests was of a maximum probability error of 5%. Results Surgery did not lead to significant improvement in histological and endoscopic findings. However, on histomorphometry, there was a significant decrease in the area, diameter and thickness of the vessels in mucosa at all colonic sites. Conclusion Surgery for decompression of schistosomal portal hypertension has a beneficial effect on the associated colopathy, being best indicated in patients with gastrointestinal bleeding and esophageal varices.
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Stacchi, Claudio, Teresa Lombardi, Francesco Oreglia, Andrea Alberghini Maltoni, and Tonino Traini. "Histologic and Histomorphometric Comparison between Sintered Nanohydroxyapatite and Anorganic Bovine Xenograft in Maxillary Sinus Grafting: A Split-Mouth Randomized Controlled Clinical Trial." BioMed Research International 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/9489825.

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The presence of vital bone after maxillary sinus augmentation is crucial to enhance the quality of bone-implant interface, ensuring predictable long-term results. The aims of this RCT with split-mouth design were the histologic and histomorphometric comparison of two different biomaterials in sinus elevation after 6 months of healing and the evaluation of the clinical outcomes of implants inserted in the augmented areas after 12 months of prosthetic loading. Twenty-eight patients (10 females, 18 males) were treated with bilateral sinus floor elevation with lateral approach. Pure sintered nanohydroxyapatite (NHA) and anorganic bovine bone (ABB) were used as test and active control, respectively. After six months, 52 bone biopsies were harvested from 26 patients, and 107 implants were inserted in the augmented areas. Histomorphometry showed that, in the two groups, vital bone percentages were 34.9±15% (NHA) and 38.5±17% (ABB) (p=0.428), marrow spaces percentages were 44.5±18% (NHA) and 43.5±23% (ABB) (p=0.866), and residual graft percentages were 20.6±13% (NHA) and 22.3±12% (ABB) (p=0.638). After 6 months of healing, no statistically significant difference was present in histomorphometric outcomes between NHA and ABB groups. Implant survival rate in NHA group after 12 months of loading was 96.4%, showing no statistically significant differences with ABB group.
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11

Kondo, Naoki, Noriaki Yamamoto, Kei Watanabe, and Naoto Endo. "Bone Histomorphometric Findings in Ankylosing Spondylitis: A Case Report." Journal of Bone Biology and Osteoporosis 4, no. 1 (December 26, 2018): 132–37. http://dx.doi.org/10.18314/jbo.v4i1.1511.

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There is minimal information on bone Histomorphometric characteristics in ankylosing spondylitis. We here report a case of a 36-year-old Japanese male that suffered from lumbago and could not gaze in the forward direction. Ultimately, a diagnosis of ankylosing spondylitis was made, and vertebroplasty was performed to correct the third lumbar spine. Histomorphometry of the iliac bone showed reduced bone volume parameters (bone volume, and trabecular thickness and width) than reference values. In addition, bone formation parameters (osteoid thickness and osteoblast surface per bone surface) and bone resorption parameters (eroded surface per bone surface and osteoclast number per bone surface) were also lower than reference values, indicating low bone turnover. By contrast, there was not a clear trend in bone resorption markers: bone- pecific alkaline phosphatase (17 U/l) was normal, TRACP-5b (136 mU/dl) was slightly lower, urinary N-terminal telopeptide (45.3 nmol BCE/mmol Cr) was normal, and deoxypyridinoline (9.1 nM/mM Cre) was higher than reference values. However, there was deficiency in 25-hydroxy vitamin D (25-OH-D; 14.4 ng/ml). This case highlights the rare possibility of performing bone histomorphometry, and indicates that a low bone volume and low bone turnover (in both bone formation and resorption) are characteristics of ankylosing spondylitis, although bone formation markers (bone-specific alkaline phosphatase) and bone mineral density are within the normal range. The possibility of a serum 25-OH-D deficient status in ankylosing spondylitis should be further considered.
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12

Molon, Rafael Scaf de, Wagner Nunes de Paula, Rubens Spin-Neto, Mario Henrique Arruda Verzola, Guilherme Monteiro Tosoni, Raphael Carlos Comelli Lia, Gulnara Scaf, and Elcio Marcantonio Jr. "Correlation of Fractal Dimension with Histomorphometry in Maxillary Sinus Lifting Using Autogenous Bone Graft." Brazilian Dental Journal 26, no. 1 (February 2015): 11–18. http://dx.doi.org/10.1590/0103-6440201300290.

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The aim of this study was to determine the pattern of bone remodeling after maxillary sinus lifting in humans by means of fractal dimension (FD) and histomorphometric analysis. Therefore, the correlation between FD and the histomorphometric findings was evaluated. Sixteen patients with posterior edentulous maxilla were enrolled in this study. Maxillary sinus lifting was performed using autogenous bone grafted from the mandibular retromolar area. Three direct digital panoramic radiographs were obtained: before surgery (Group 1), immediately postoperatively (Group 2) and after 6 months of healing (Group 3) for FD analysis. Biopsies were taken after 6 months, processed and submitted to histological and histomorphometric analysis. Data were analyzed by Shapiro-Wilk test and ANOVA test followed by a Tukey test (a=0.05). The bone volume fraction of newly trabecular bone (TB) and medullary area (MA) was measured as 62.75%±17.16% and 37.25±17.16%, respectively. Significant difference in FD analysis was measured between Group 1 and Group 3. No significant difference was found in the correlation between FD and histomorphometric analysis for TB and MA (p=0.84). In conclusion, all performed analyses were effective in assessing the bone-remodeling pattern in the maxillary sinus, offering complementary information about healing and predictable outcomes. There were no correlations between FD and histomorphometric analysis.
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Alfonsi, Fortunato, Niccolò Baldi, Stefano Romeggio, Paolo Tonelli, Giovanna Iezzi, Eugenio Velasco-Ortega, and Antonio Barone. "Histomorphometric and Clinical Analysis of Ridge Preservation Procedures in Extraction Sockets with Buccal Bone Defects (>5mm) Using an In Situ Hardening Biphasic Calcium Phosphate (HA/β-TCP) Graft and a Bioresorbable Matrix: A Human Study at 6 Months." Applied Sciences 10, no. 9 (April 27, 2020): 3034. http://dx.doi.org/10.3390/app10093034.

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Many biomaterials have been proposed for ridge preservation techniques to counteract fresh extraction socket resorption. The primary aim of this prospective single cohort study was to evaluate the histomorphometric outcomes of a synthetic biphasic calcium phosphate (60% HA/40% β-TCP) and a synthetic poly-lactic acid membrane, used to graft fresh extraction socket sites with a full or partial (>5mm) resorption of the buccal bone plate. Patients recruited were treated at one esthetic site with a ridge preservation procedure to receive an implant-supported prosthesis. After 6 months of healing, a bone biopsy was harvested. Outcome evaluations were: biological complications, histomorphometrical analysis, and alveolar horizontal and vertical bone loss (ΔAHB, ΔAVB). Thirteen subjects were included in this study. Two cases of biological complication were recorded. All 13 patients received implant insertion. From histomorphometric analysis, a mean of 48.9 ± 11.9%, 29.0 ± 9.3%, and 22.0 ± 9.7% was recorded for soft tissues, new bone, and residual graft particles, respectively. From clinical analysis, a mean of 0.5 ± 1.0 mm (p-value < 0.05) and 0.9 ± 1.3 mm (p-value < 0.05) was recorded for alveolar horizontal and vertical bone loss, respectively. In conclusion, this prospective cohort study showed encouraging results in preserving alveolar ridge dimension. A moderate percentage of new bone and an acceptable alveolar ridge loss were achieved at a 6 month follow-up.
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Freitas, Luana Azevedo de, Fábio Roger Vasconcelos, Arlindo Alencar Araripe Noronha Moura, Stefanie Bressan Waller, Paula Priscila Correia Costa, Brenda Madruga Rosa, Wesley Lyeverton Correia Ribeiro, and Lúcia Daniel Machado da Silva. "Histomorphometry and uterine proteomics during the normal reproductive cycle in bitches." Research, Society and Development 10, no. 11 (August 22, 2021): e18101119093. http://dx.doi.org/10.33448/rsd-v10i11.19093.

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We aimed to evaluate the histomorphometry and proteomic profile of the canine uterus during all stages of the reproductive cycle. Eighteen healthy female dogs had their estrous cycle identified by clinical evaluation, vaginal cytology, and serum progesterone levels, which were allocated to the proestrus (n=5), estrus (n=5), diestrus (n=5), and anestrus (n=3) groups. All were submitted to elective ovariosalpingohysterectomy, and the uteri were collected for histomorphometric measurement (Image J software). For proteomic analysis, fragments of the uterine horns were subjected to protein measurement (Bradford method) and extraction by 2D electrophoresis (PDquest software). The results showed that the diestrus promoted greater values of thickness in the uterine structures (μm): uterine wall (2,223.8±229.8), endometrium (819.7±109.1), and myometrium (1,392.6±294.2). Uterus showed a protein profile with good reproducibility per phase (pI: 3.5–9.0; PM: 24–150 KDa), with 11 spots in all phases. Despite the greatest histomorphometric changes in the diestrus, we observed a greater number of spots in the estrus (253±45), followed by the proestrus (185±21), diestrus (113±39), and anestrus (80±21). This finding showed probable participation of these proteins in the uterine preparation for receiving gametes for fertilization. Our results showed greater uterine thickness in the diestrus, and greater protein secretion in the estrus, contributing to the prospection of identification of proteins responsible for the biological reproduction processes.
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La Monaca, Gerardo, Giovanna Iezzi, Maria Paola Cristalli, Nicola Pranno, Gian Luca Sfasciotti, and Iole Vozza. "Comparative Histological and Histomorphometric Results of Six Biomaterials Used in Two-Stage Maxillary Sinus Augmentation Model after 6-Month Healing." BioMed Research International 2018 (June 27, 2018): 1–11. http://dx.doi.org/10.1155/2018/9430989.

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Objectives. To evaluate the performances of six different bone substitute materials used as graft in maxillary sinus augmentation by means of histological and histomorphometric analysis of bone biopsies retrieved from human subjects after a 6-month healing period. Materials and Methods. Six consecutive patients (3 males, 3 females, aged 50-72 years), healthy, nonsmokers, and with good oral hygiene, presenting edentulous posterior maxilla with a residual bone crest measuring ≤ 4 mm in vertical height and 3 to 5 mm in horizontal thickness at radiographic examination, were selected to receive sinus augmentation and delayed implant placement. Under randomized conditions, sinus augmentation procedures were carried out using mineralized solvent-dehydrated bone allograft (MCBA), freeze-dried mineralized bone allograft (FDBA), anorganic bovine bone (ABB), equine-derived bone (EB), synthetic micro-macroporous biphasic calcium-phosphate block consisting of 70% beta-tricalcium phosphate and 30% hydroxyapatite (HA-β-TCP 30/70), or bioapatite-collagen (BC). After 6 months, bone core biopsies were retrieved and 13 implants were placed. Bone samples were processed for histological and histomorphometric analysis. CT scans were taken before and after surgery. After 4 months of healing, patients were restored with a provisional fixed acrylic resin prosthesis, as well as after further 2-4 months with a definitive cemented zirconia or porcelain-fused-to-metal crowns. Results. There were no postoperative complications or implant failures. The histological examination showed that all biomaterials were in close contact with newly formed bone, surrounding the graft granules with a bridge-like network. No signs of acute inflammation were observed. The histomorphometry revealed 20.1% newly formed bone for MCBA, 32.1% for FDBA, 16.1% for ABB, 22.8% for EB, 20.3% for HA-β-TCP 30/70, and 21.4% for BC. Conclusions. Within the limitations of the present investigation, all the six tested biomaterials showed good biocompatibility and osteoconductive properties when used in sinus augmentation procedures, although the FDBA seemed to have a better histomorphometric result in terms of newly formed bone and residual graft material. This trial is registered with ClinicalTrials.gov Identifier (Registration Number): NCT03496688.
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Mechery, Reenesh, Manish Mukherjee, and AK Shreehari. "Histomorphometric analysis of bone interphase." Journal of Dentistry Defence Section 14, no. 2 (2020): 59. http://dx.doi.org/10.4103/jodd.jodd_19_20.

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Hayat Niazi, Sikander. "Histomorphometric study of vermiform appendix." Professional Medical Journal 26, no. 10 (October 10, 2019): 1635–39. http://dx.doi.org/10.29309/tpmj/2019.26.10.2919.

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Objectives: To understand the histopathology of various diseases of vermiform appendix, the knowledge of normal histology of the organ at different age group is mandatory. Study Design: Comparative study. Setting: Islamic International Medical College Rawalpindi. Period: January 2014 to March 2015. Material and Methods: Total forty negative appendectomy/normal appendices specimens removed along with other abdominal operations were included in this study. Four equal groups (10 specimens in each) were made, spacing 15 years between each group. The last group had no upper age limit due to less availability of the specimens. The middle parts of specimens were included in this study the various parameters i.e. wall thickness, lymphoid nodules and lumen sizes were measured under microscope after calibration in micrometers after staining. Results: The lumen size decreases with advancing age. There was inverse relationship between lumen size and wall thickness. Surprisingly sum of mean lumen size and mean wall thickness of all age groups had no much difference. The number and size of lymphoid nodules decreases with advancing age. Conclusion: Although lumen size decreased as age advances but did not obliterate completely till 74 years age. The number and size of lymphoid nodule decreased but wall thickness size remained same and they are observed even at the age of 74 years.
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Rao, Trisi, and Walter Rao. "Bone classification: clinical-histomorphometric comparison." Clinical Oral Implants Research 10, no. 1 (February 1999): 1–7. http://dx.doi.org/10.1034/j.1600-0501.1999.100101.x.

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Merli, Mauro, Giorgia Mariotti, Annalisa Mazzoni, Marco Moscatelli, Umberto Pagliaro, and Michele Nieri. "The Wafer Technique: Histomorphometric Results." International Journal of Periodontics & Restorative Dentistry 40, no. 6 (November 2020): 815–23. http://dx.doi.org/10.11607/prd.4280.

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Zivkovic-Marinkov, Emilija, Milan Stankovic, Dragan Mihailovic, and Mila Bojanovic. "Correlation between histomorphometric changes and the type of aerobic bacteria isolated in chronic suppurative otitis media." Vojnosanitetski pregled 68, no. 1 (2011): 46–50. http://dx.doi.org/10.2298/vsp1101046z.

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Background/Aim. Bacterial flora is a very important factor in pathogenesis of chronic suppurative otitis media (CSOM) and significantly influences the type and intensity of osteolytic process. There are few histomorphometric investigations of middle ear mucosa in chronic otitis. The aim of this study was to identify aerobic bacteria responsible for chronic suppurative otitis media as well as their association with histomorphometric changes of middle ear mucosa. Methods. A prospective study that comprised 153 patients treated in the Clinc for Ear, Thorat and Nose Diseases, Nis, was conducted. Bacteriologic analysis of diseased ear secretion was carried out in all patients. Intraoperatively removed granulation tissue was used for histomorphometry. The analysed parameters were: the number of inflammatory cells, as well as vascularization and vasodilatation. Results. The most frequently isolated aerobic bacteria from chronic suppurative otitis media were Staphylococcus aureus (29.02%), Pseudomonas aeruginosa (29.02%) and Proteus spp. (21.76%). There was no correlation between the type of pathologic process and the type of bacteria.The number of inflammatory cells in the granulation tissue in pure cultures of Staphylococcus aureus was 1,597.33 ? 549.45 and in Pseudomonas auriginosa cultures was 2,639 ? 648. Conclusion. This study showed that there is a statistically significant correlation between the number of inflammatory cells in the granulation tissue and the type of aerobic bacteria we isolated. The intensity of the infection in chronic suppurative otitis media depends on the type of the isolated bacteria, which emphasizes the importance of adequate preoperative antimicrobial therapy.
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Ustun, Yakup, Ozgur Erdogan, Mehmet Kurkcu, Tolga Akova, and Ibrahim Damlar. "Effects of Low-Intensity Pulsed Ultrasound on Dental Implant Osseointegration: A Preliminary Report." European Journal of Dentistry 02, no. 04 (October 2008): 254–62. http://dx.doi.org/10.1055/s-0039-1697389.

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ABSTRACTObjectives: The aim of this pilot study was to evaluate the effects of low intensity pulsed ultrasound (LIPU) on dental implant osseointegration in a rabbit model using mechanical-histomorphometric methods and resonance-frequency analysis (RFA).Methods: Twelve skeletally mature, male New Zealand rabbits (3.4 kg±0.5) were included in the study. A total number of 24 implants were placed bilaterally into the tibiae of the subjects. The right tibia of each rabbit received LIPU application (20 min/day) while the left side received sham treatment. The study was carried on for six weeks and the rabbits were sacrificed in 7 days intervals (two rabbits for each week). The rabbits were categorized in the early or late osseointegration period according to their sacrification date. Comparisons between the groups were made using statistical analysis of histomorphometric analysis, resonance frequency analysis and mechanical tests.Results: The histomorphometry parameters showed that the bone area and the bone volume values have significantly increased in the early osseointegration period and the bone-implant contact values have significantly increased in the late osseointegration period in the LIPU treated subjects when compared to the control group. RFA scores had mild increase in the LIPU group. However the difference was not statistically significant. Mechanical test results suggest an increased mechanical stability in the LIPU group as well.Conclusions: Results of this pilot study indicate that low intensity pulsed ultrasound may have positive effects on osseointegration and stability of dental implants. (Eur J Dent 2008;2:254-262)
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Schnutenhaus, Sigmar, Werner Götz, Jens Dreyhaupt, Heike Rudolph, Ralph G. Luthardt, and Cornelia Edelmann. "Associations among Primary Stability, Histomorphometric Findings, and Bone Density: A Prospective Randomized Study after Alveolar Ridge Preservation with a Collagen Cone." Dentistry Journal 8, no. 4 (October 2, 2020): 112. http://dx.doi.org/10.3390/dj8040112.

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Background: The objective of this investigation was to examine whether determination of bone density (BD) with a cone beam computed tomography (CBCT) scan could help predict the primary stability (PS) of the implants and to investigate whether associations between the histomorphometric findings and the CBCT scan could be observed. Materials and methods: In this randomized clinical study, the efficacy of alveolar ridge preservation (ARP) with a combination of a collagen cone and a collagen membrane procedure after tooth extraction was investigated. CBCT scans were obtained after a healing period of 8 (±1) weeks. Subsequently, the CBCT scans were evaluated in terms of BD at different heights of the former socket. Eleven (±1) weeks after tooth extraction, implant placement was performed and PS was measured with resonance frequency analysis. Potential associations among the radiologically measured BD, the histomorphometric results, and the PS were analyzed. Results: No direct association was observed between the radiologically determined BD and the histomorphometric findings. No significant associations could be found between the BD and the PS. Conclusion: No significant associations were observed among the BD determined by the CBCT, the histomorphometric findings, and the PS.
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Erdogan, Özgür, Yakup Üstün, Ufuk Tatli, Ibrahim Damlar, and Kenan Daglıoglu. "A Pig Model for the Histomorphometric Evaluation of Hard Tissue Around Dental Implants." Journal of Oral Implantology 39, no. 5 (October 1, 2013): 551–57. http://dx.doi.org/10.1563/aaid-joi-d-11-00009.

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This study aimed to evaluate the frontal bone of Swiss Domestic pigs as an animal model for the histologic-histomorphometric examination of bony tissue around dental implants. We inserted SLA surface implants 4.1 mm in diameter and 10 mm in length into the frontal bones of 9 Swiss-Domestic pigs. Histologic and histomorphometric studies were conducted on the undecalcified sections. Histologic examinations showed that the specimens contain a sufficient amount of bone to provide homogenous bone coverage for standard diameter dental implant placement. The mean bone to implant contact was 61.9% ± 8.7%. Other histomorphometric parameters revealed the regular trabecular architecture at this site. Pigs' frontal bone appears to be a suitable animal model in short-term dental implant studies because it provides a sufficient amount of bone and favorable bone microarchitecture.
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Vastardis, H., J. B. Mulliken, and J. Glowacki. "Unilateral Coronal Synostosis: A Histomorphometric Study." Cleft Palate-Craniofacial Journal 41, no. 4 (July 2004): 439–46. http://dx.doi.org/10.1597/03-012.1.

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Objective This histomorphometric study compared the open and prematurely fused side of the coronal suture in subjects with unilateral coronal synostosis (UCS). Methods Sutures and parasutural bone were obtained from seven subjects with nonsyndromic UCS during operative correction at 3 to 24 months of age. Histological and cellular analyses were performed for the affected and open sutures. Specimens were examined by light and polarizing microscopy. Sutural patterns, osseous morphology, calvarial thickness, tartrate-resistant acid phosphatase (TRAP)-positive cells, and marrow spaces were evaluated histomorphologically, qualitatively, and semiquantitatively. Histomorphometry was performed to determine total projected area of marrow space as a percentage of unit area, total number of TRAP-positive cells per specimen, and perisutural cranial thickness. Results Polarizing microscopy showed that affected sutures were composed of more lamellar bone than the normal sutures. By light microscopy, the clinically fused sutures were 1.7-fold thicker (p < .02), had twofold larger marrow spaces (p < .0006), and contained sixfold more TRAP-positive osteoclasts in marrow spaces near the suture (p < .04) than the normal sutures. Quantitative analysis of the normal sutures revealed that calvarial thickness was greater with age and that there was an inverse correlation between medullary area and age. For the affected sutures, there was also an age-related increase in calvarial thickness. There were also trends for age-related declines in numbers of osteoclasts in both open and affected sides. Conclusions These results question the hypothesis that defective osteoclastic activity is pivotal in the pathogenesis of UCS and support the hypothesis that this condition results from abnormally active bony remodeling.
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Mangano, Francesco, Carlo Mangano, Adriano Piattelli, and Giovanna Iezzi. "Histological Evidence of the Osseointegration of Fractured Direct Metal Laser Sintering Implants Retrieved after 5 Years of Function." BioMed Research International 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/9732136.

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Background. Direct metal laser sintering (DMLS) is an additive manufacturing technique that allows the fabrication of dental implants layer by layer through the laser fusion of titanium microparticles. The surface of DMLS implants is characterized by a high open porosity with interconnected pores of different sizes; therefore, it has the potential to enhance and accelerate bone healing. To date, however, there are no histologic/histomorphometric studies in the literature evaluating the interface between bone and DMLS implants in the long-term. Purpose. To evaluate the interface between bone and DMLS implants retrieved after 5 years of functional loading. Methods. Two fractured DMLS implants were retrieved from the human jaws, using a 5 mm trephine bur. Both the implants were clinically stable and functioned regularly before fracture. The specimens were processed for histologic/histomorphometric evaluation; the bone-to-implant contact (BIC%) was calculated. Results. Compact, mature lamellar bone was found over most of the DMLS implants in close contact with the implant surface; the histomorphometric evaluation showed a mean BIC% of 66.1% (±4.5%). Conclusions. The present histologic/histomorphometric study showed that DMLS implants were well integrated in bone, after 5 years of loading, with the peri-implant bone undergoing continuous remodeling at the interface.
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Gadek, Artur, Leszek Wojnar, and Edward Czerviński. "EFFECT OF HISTOMORPHOMETRIC PARAMETERS ON COMPRESSION STRENGTH OF VERTEBRAL BODIES." Image Analysis & Stereology 20, no. 1 (May 3, 2011): 35. http://dx.doi.org/10.5566/ias.v20.p35-39.

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Computer aided image analysis was applied to elaborate an automatic method of histomorphometric analysis of trabecular bone samples. Transverse sections of decalcified vertebral bodies were examined using optical microscopy and digital image acquisition system. Further analysis was done by means of a general purpose image analysis package. The same algorithm was applied to all the images tested, thus enabling obtainment of objective and repeatable results. High efficiency in measurements and evaluation of parameters not accessible for manual methods makes this method an interesting alternative for classical histomorphometric analysis. The results obtained demonstrated that assessment of bone mineral density is not sufficient for evaluation of compression strength of vertebral bodies. In contrast, mechanical properties correlate well with histomorphometric parameters. As a consequence it was postulated that compression strength of vertebral bodies is controlled by trabecular structure rather than bone mineral density.
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Rubio, A., M. R. Fernández, J. Figols, and J. Rama. "Experimental study on neurorrhaphy of the recurrent laryngeal nerve in dogs." Journal of Laryngology & Otology 110, no. 8 (August 1996): 748–53. http://dx.doi.org/10.1017/s0022215100134863.

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AbstractThe effectiveness of anastomosis of a divided recurrent laryngeal nerve was evaluated in six adult mongrel dogs. Videolaryngoscopy and evoked compound muscle action potentials in the intrinsic laryngeal muscles were performed at six months and the posterior cricoarytenoid muscles and recurrent laryngeal nerves were processed for histomorphometric studies. Recovery of compound muscle action potentials in all re-innervated muscles and histomorphometric findings confirmed a good grade of axonal regeneration. The most significant histomorphometric changes observed were: a reactive hypertrophy of type I fibres in the posterior cricoarytenoid muscles of the re-innervated side, and a high nerve fibre density in the distal stump to the anastomosis. However, incomplete recovery of motion and fasciculated movements of the reinnervated vocal folds were observed. Reduction of effective motor units in the re-innervated muscles might be a factor that cause incomplete restoration of vocal fold movements.
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Minetti, Elio, Edoardo Giacometti, Ugo Gambardella, Marcello Contessi, Andrea Ballini, Gaetano Marenzi, Martin Celko, and Filiberto Mastrangelo. "Alveolar Socket Preservation with Different Autologous Graft Materials: Preliminary Results of a Multicenter Pilot Study in Human." Materials 13, no. 5 (March 5, 2020): 1153. http://dx.doi.org/10.3390/ma13051153.

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Background: The histological and histomorphometrical results were evaluated between vital whole and non-vital endodontically treated teeth used as autologous grafts in post-extractive socket preservation procedures. Methods: Twenty-eight patients (average age 51.79 ± 5.97 years) with post-extractive defects were enrolled in five dentistry centers. All patients were divided into two groups: with whole teeth (Group 1) and teeth with endodontical root canal therapy (Group 2). The extracted teeth were processed with the Tooth Transformer device to obtain a demineralized and granulated graft material used with a resorbable collagen membrane for socket preservation. After four months, 32 bone biopsies were obtained for histological, histomorphometric, and statistical analysis. Results: During the bone healing period, no infection signs were observed. Nineteen biopsies in group 1 and 13 biopsies in group 2 were detected. The histological analysis showed neither inflammatory nor infective reaction in both groups. Autologous grafts surrounded by new bone were observed in all samples and, at high magnification, partially resorbed dentin and enamel structures were detected. No gutta-percha or cement was identified. Small non-statistically significant differences between the groups, in total bone volume (BV), autologous graft residual, and vital bone percentage were detected. Conclusions: The study showed that the TT Transformer grafts were capable of producing new vital bone in socket preservation procedures. The histomorphometric results showed no statistical differences comparing whole and endodontically treated teeth in bone regeneration. Further studies will be carried out in order to understand the advantages of the autologous graft materials obtained from the tooth compared with the current biomaterials in bone regeneration treatments.
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Neto, Ricardo, Luciano Pereira, Juliana Magalhães, Janete Quelhas-Santos, and João Frazão. "Low bone turnover is associated with plain X-ray vascular calcification in predialysis patients." PLOS ONE 16, no. 10 (October 13, 2021): e0258284. http://dx.doi.org/10.1371/journal.pone.0258284.

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Background Vascular calcification (VC) is a common finding in chronic kidney disease (CKD) patients and predicts subsequent cardiovascular morbidity and mortality in this population. Vascular calcification is linked to disordered mineral metabolism and has been associated with bone histomorphometry changes in CKD. However, data on predialysis patients is scarce. Methods A cross-sectional study was conducted on a cohort of 56 CKD patients not yet on dialysis, who underwent a transiliac bone biopsy for histomorphometric evaluation after double tetracycline labeling. Patients had no previous exposure to calcium salts, vitamin D agents, steroids or bisphosphonates. Vascular calcification was assessed at the time of biopsy, using Kauppila (plain X-ray of the lateral lumbar spine) and Adragão (plain X-ray of the pelvis and hands) scores. Results Vascular calcification was seen in two-thirds of the cohort. Subjects with VC were more likely to be male and have diabetes, and had significantly higher sclerostin and osteoprotegerin circulating levels than those without VC. The histomorphometric analysis showed that bone formation rate was significantly lower in VC compared to non-VC patients. In the multivariable logistic regression analysis, bone formation rate was independently associated with the presence of VC. Conclusions Vascular calcification is highly prevalent in predialysis patients, especially in those with diabetes. The independent association between bone formation rate and VC provides evidence of an important interaction between bone and vessel in CKD. Our results suggest that low bone turnover is a non-traditional risk factor for cardiovascular disease in predialysis patients.
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Yarram, S. J., M. J. Perry, T. J. Christopher, K. Westby, N. L. Brown, T. Lamminen, S. B. Rulli, et al. "Luteinizing Hormone Receptor Knockout (LuRKO) Mice and Transgenic Human Chorionic Gonadotropin (hCG)-Overexpressing Mice (hCG αβ+) Have Bone Phenotypes." Endocrinology 144, no. 8 (August 1, 2003): 3555–64. http://dx.doi.org/10.1210/en.2003-0036.

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Abstract Considerable attention has been paid to the role of sex steroids during periods of major skeletal turnover, but the interaction of the gonadotropic hormones, which include LH, FSH, and human chorionic gonadotropin (hCG), within bone tissue have been overlooked. The question is pertinent due to the recent detection of extragonadal expression of gonadotropin receptors. Western blotting, immunolocalization, and RT-PCR supported the presence of osteoblast LH receptors. However, osteoblast cells failed to bind [125I]hCG and treatment with hCG failed to generate either cAMP or phosphorylated ERK 1/2. Bone mineral density (BMD) and bone histomorphometry were examined in the following models: 1) LH receptor null mutant (LuRKO) mice; 2) transgenic mice overexpressing hCG (hCG αβ+); and 3) ovariectomized (OVX) hCG αβ+ model. Male LuRKO mice showed a decrease in BMD after 5 months, apparently secondary to suppressed gonadal steroid production. Similarly, 9- to 10-wk-old female LuRKO mice exhibited decreases in histomorphometric parameters tested. The data indicate that loss of LH signaling results in a reduction in bone formation or an increase in bone resorption. By contrast, there were significant increases in BMD and histomorphometric indices for female, but not male, hCG αβ+ mice, indicating that chronic exposure to hCG results in bone formation or a decrease in bone resorption. However, OVX of the hCG αβ+ mice resulted in a significant reduction in BMD comparable to OVX WT controls. Although gonadotropin levels are tightly linked to sex steroid titers, it appears that their effects on the skeleton are indirect.
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Villanueva, A. R., and A. S. Turner. "Static and Dynamic Histomorphometric Data in 9- to 11-Year-Old Ewes." Veterinary and Comparative Orthopaedics and Traumatology 07, no. 03 (1994): 101–9. http://dx.doi.org/10.1055/s-0038-1633129.

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SummaryThe technique of trans-iliac biopsy and subsequent histomorphometrical examination in ewes is reported. Biopsies were obtained from 18 clinically normal ewes, aged 9 to 11 years which had been given tetracycline intramuscularly twice, 11 days apart, at a dose rate of 20 mg/kg. There were no complications associated with the administration of the tetracycline at this dose and at this site. The core of the biopsy consisted of cancellous bone between two cortices allowing complete histomorphometric analysis from undecalcified sections. Two clearly distinct and separate labels were visible under ultraviolet light with the labelling method used. Primary, surface, volume and dynamic data were measured or calculated and are reported. Certain static and dynamic histomorphometric variables from this study were compared to those of other workers who used aged (but slightly younger) ewes, and there were many similarities between the studies. A comparison was also made of absolute values of bone volume (BV/TV%), relative osteoid volume (OV/BV%) and mineral apposition rate (MAR, mcm/day) of this study, and those of men and pre- and postmenopausal women reported in recently published data, as well as parameters of 14 elderly normal women measured by one of us. It was concluded that iliac crest biopsy in the ewe is a safe effective method to quantitate skeletal changes and our data compares favorably with other workers in this species. Furthermore, the BV/TV%, OV/BV% and MAR; of ewes of this age resembled that of both men and postmenopausal women at least in their 6th or 7th decade of life. Therefore 9- to 11-year-old ewes may be a useful models for studies and therapeutic trials of both osteopenia and osteoporosis of the skeleton.The article describes the technique of trans-iliac biopsy and subsequent histomorpho-metrical examination in aged ewes.
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Alim, Abdul, Abu Sadat Mohammad Nurunnabi, Sabiha Mahbub, and Shamim Ara. "Histomorphometric study of the human spleen." Bangladesh Journal of Medical Science 11, no. 4 (November 13, 2012): 298–302. http://dx.doi.org/10.3329/bjms.v11i4.12600.

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Context: The spleen is the largest single mass of lymphoid tissue in all vertebrates. Histological changes are evident in advancing age along with functional capability of the human spleen. A cross-sectional, descriptive type of study was done in the Department of Anatomy, Dhaka Medical College, Dhaka, from January to December 2008, to see the age related microscopic changes in the human spleen. Methods: 30 human spleens were collected from the unclaimed dead bodies that were under examination in the Department of Forensic Medicine of Dhaka Medical College, Dhaka. The samples were divided into three age-groups including group A (15-29 years), group B (30-49 years) & group C (50-69 years). Then 5 best prepared histological slides from each group were taken and examined under the light compound microscope to determine the thickness of capsule, diameter, number and percent amount of white pulp in the human spleen. Results: The thickness of the splenic capsule were found 84.60±7.97 ?m, 117.92±4.16 ?m, and 132.17±6.37 ?m in group A, B and C respectively. The diameter of the white pulp were found 0.32±0.01 mm, 0.32±0.01 mm and 0.31±0.01 mm, while the number of white pulp per sq. mm were 2.28±1.04, 2.38±0.93 and 2.04±0.76 in group A, B and C respectively. Moreover, the amounts of the white pulp were determined 23.09±1.38, 24.45±1.84 and 22.54±1.08 in group A, B and C respectively. The difference in thickness of the splenic capsule was statistically significant among the study groups (p<0.001). However, no difference was found in diameter, number and percentage of the white pulp of the spleen among those age groups. Conclusion: The thickness of the capsule of the spleen was found to increase with advancing age in humans. However, no age change was evident in diameter, number or amount of the white pulp. DOI: http://dx.doi.org/10.3329/bjms.v11i4.12600 Bangladesh Journal of Medical Science Vol. 11 No. 04 Oct’12
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33

Schnutenhaus, Sigmar, Werner Götz, Jens Dreyhaupt, Heike Rudolph, and Ralph G. Luthardt. "Alveolar Ridge Preservation: A Histomorphometric Analysis." Open Dentistry Journal 12, no. 1 (October 30, 2018): 916–28. http://dx.doi.org/10.2174/1874210601812010916.

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Objective: This study presents the histomorphometric findings after tooth extraction with and without Alveolar Ridge Preservation (ARP) with a collagen cone filling the socket in combination with a collagen membrane covering the socket. Materials and Methods: In a controlled randomized clinical study, 10 patients were treated with the combination material after tooth extraction. In 10 patients, the extraction sockets were left to heal without further intervention. Soft tissue, new bone formation, bone quality and bone remodeling, blood flow vascularization, and inflammation were evaluated histomorphometrically. This was performed (semi-) quantitatively using a blinded protocol. Results: The statistical evaluation showed no significant difference for any parameter. When the combination material was used, more pronounced remodeling, increased osteoblast activity, and increased vascularization were demonstrated based on the histomorphometric findings. In contrast, there were reduced levels of osteogenesis and less mineralization. There was slightly more bundle bone in patients with ARP. Conclusion: The histomorphometric analysis of ARP with a combination material consisting of a collagen cone and a collagen membrane showed no significant differences in terms of new bone formation and bone quality. Descriptively, however, different manifestations were seen that might benefit from being documented using larger samples and being tested for clinical relevance.
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34

Curvo, Lucimar Rodrigues Vieira, Milena Wolff Ferreira, Celso Soares Costa, Guilherme Ribeiro Capibaribe Barbosa, Sandra Adriana Uhry, Ulisses Simon da Silveira, Alanderson Rodrigues da Silva, and Gisele Braziliano de Andrade. "Techniques using ImageJ for histomorphometric studies." Research, Society and Development 9, no. 11 (November 11, 2020): e1459119586. http://dx.doi.org/10.33448/rsd-v9i11.9586.

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Computational histomorphometry is an available and easy tool that has been used in the assessment of morphophysiological tissue changes, offering greater scientific reliability to the data, as well as facilitating the automation process. The present work aimed to describe the application of the methodology of the free software ImageJ for morphological evaluation of fish tissues. For this, micrographs of histological sections of the intestinal tract of fish stained with Periodic Acid-Schiff (PAS) were used as a model. The images were analyzed for variables of length, width, and tissue area and, number of cells or molecules. The application of computational histomorphometry demonstrated efficiency in the evaluation of histological structures of the intestine of fish supplemented with probiotics, contributing to the improvement of image analysis techniques in animal tissue models.
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Vande Vannet, B., M. M. Sabzevar, H. Wehrbein, and K. Asscherickx. "Osseointegration of miniscrews: a histomorphometric evaluation." European Journal of Orthodontics 29, no. 5 (October 1, 2007): 437–42. http://dx.doi.org/10.1093/ejo/cjm078.

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36

Slete, Frederic B., Paul Olin, and Hari Prasad. "Histomorphometric Comparison of 3 Osteotomy Techniques." Implant Dentistry 27, no. 4 (August 2018): 424–28. http://dx.doi.org/10.1097/id.0000000000000767.

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37

Parchami, A., and R. H. . Fatahian. "Histomorphometric Study of Sheep Fetal Testis." Asian Journal of Biological Sciences 1, no. 2 (June 15, 2008): 90–93. http://dx.doi.org/10.3923/ajbs.2008.90.93.

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38

Vialle, Emiliano Neves, Luiz Roberto Gomes Vialle, and André de Oliveira Arruda. "Histomorphometric Analysis of Experimental Disc Degeneration." Global Spine Journal 2, no. 3 (September 2012): 129–35. http://dx.doi.org/10.1055/s-0032-1326951.

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39

Misra, Vatsala, Sri Prakash Misra, Manisha Dwivedi, and Suresh C. Gupta. "Histomorphometric Study of Portal Hypertensive Enteropathy." American Journal of Clinical Pathology 108, no. 6 (December 1, 1997): 652–57. http://dx.doi.org/10.1093/ajcp/108.6.652.

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40

Pellicioli, Ana Carolina Amorim, Felipe Paiva Fonseca, Rodrigo Neves Silva, Luiz Alcino Monteiro Gueiros, Oslei Paes de Almeida, Pablo Agustin Vargas, Marcio Ajudarte Lopes, et al. "Histomorphometric characterization of subgemmal neurogenous plaques." Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 123, no. 4 (April 2017): 477–81. http://dx.doi.org/10.1016/j.oooo.2016.12.014.

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BARBOSA, JÉSSICA BARROSO, and TIAGO NOVAES PINHEIRO. "HISTOMORPHOMETRIC ANALYSIS OF CHRONIC SCLEROSING SIALOADENITIS." Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology 124, no. 2 (August 2017): e124-e125. http://dx.doi.org/10.1016/j.oooo.2017.05.328.

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42

Nakano, Masataka, Fumiyuki Otsuka, Saami Yazdani, Aloke V. Finn, Frank D. Kolodgie, Narula Jagat, and Renu Virmani. "HISTOMORPHOMETRIC CHARACTERISTICS OF HIGH-RISK PLAQUES." Journal of the American College of Cardiology 57, no. 14 (April 2011): E1445. http://dx.doi.org/10.1016/s0735-1097(11)61445-9.

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43

Freemont, A. J., T. Rehman, J. A. Hoyland, and J. Denton. "Histomorphometric subtypes of postmenopausal osteoporosis (PMO)." Bone 16, no. 6 (June 1995): 685–86. http://dx.doi.org/10.1016/8756-3282(95)91662-q.

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44

Palumbo, C., G. Marotti, and M. Ferretti. "Histomorphometric study on osteocyte-osteoblasts relationships." Bone 13, no. 5 (July 1992): A26. http://dx.doi.org/10.1016/8756-3282(92)90559-f.

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45

Bonucci, Ermanno, Paola Ballanti, Carlo Della Rocca, Silvano Milani, Vincenzo Lo Cascio, and Bruno Imbimbo. "Technical variability of bone histomorphometric measurements." Bone and Mineral 11, no. 2 (November 1990): 177–86. http://dx.doi.org/10.1016/0169-6009(90)90057-m.

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46

Kwak, Hyun Ho, Byung Chan Koo, Kyung Seok Hu, Gyoo Cheon Kim, Bong Soo Park, and Hee Jin Kim. "Histomorphometric Characteristics of the Facial Nerve." Korean Journal of Physical Anthropology 21, no. 4 (2008): 343. http://dx.doi.org/10.11637/kjpa.2008.21.4.343.

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47

Yusupova, Sania, Ekaterina Kostrigina, Natalia Bulkina, Valery Konnov, Anna Vedyaeva, Larisa Zyulkina, and Petr Ivanov. "HISTOMORPHOMETRIC PARAMETERS IN SIMULATED GINGIVAL RECESSION." Archiv Euromedica 10, no. 4 (December 17, 2020): 174–75. http://dx.doi.org/10.35630/2199-885x/2020/10/4.39.

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This study addresses the nature of morphological changes occurring in periodontal tissues through the development of gum recession in the experiment. Gingival recession was found to occur along with a decrease in epithelial thickness of the mucous membrane as well as a decreasing cross-sectional area of blood vessels, whereas the connective tissue featured fibrinoid swelling. Initial tissue ischemia in the affected area can have a negative effect on reparation. This should be borne in mind when planning a periodontal plastic surgery.
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48

Dalle Carbonare, L., F. Bertoldo, M. T. Valenti, S. Zenari, M. Zanatta, S. Sella, S. Giannini, and V. Lo Cascio. "Histomorphometric analysis of glucocorticoid-induced osteoporosis." Micron 36, no. 7-8 (October 2005): 645–52. http://dx.doi.org/10.1016/j.micron.2005.07.009.

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49

Moreira, C. A., and D. W. Dempster. "Histomorphometric changes following treatment for osteoporosis." Journal of Endocrinological Investigation 40, no. 9 (May 26, 2017): 895–97. http://dx.doi.org/10.1007/s40618-017-0662-6.

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50

Castejón, Rafael, Carlos Gamallo, and Enrique Jaureguizar. "Histomorphometric study of experimental extramucosal ureteroureterostomy." Microsurgery 13, no. 1 (1992): 2–6. http://dx.doi.org/10.1002/micr.1920130103.

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