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1
Crowe, Nicole M. Crowe. "Histomorphometric Analysis of Elderly Ribs at Various Locations." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1503304250839703.
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Price, Jeff. "Growth characteristics underlying retrogenia in pigs: A histomorphometric study." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1395925724.
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Ribeiro, Henrique CÃsar TemÃteo. "Induction model of achilles tendinosis in rats: a histomorphometric study." Universidade Federal do CearÃ, 2013. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=12287.
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The tendon diseases are major causes of injury in athletes undergoing excessive training. The
tendinosis is one of these lesions and is characterized histologically for a hyper cellularity and
increased deposition of type III collagen.
There are several possible models of tendinosis
induction in animals, being most effective in the mechanical reproduction of the events
involved in this process. Because the driving characteristic of the hind legs, one incline of the
treadmill used in the 10 degrees (acclivity) induction is necessary. The running speed, which
the animals were submitted in previous models, is a factor difficult to reproduce. There is
need for standardization of model induction Achilles tendinosis, reproducible. The purpose is
to define and validate a model for the induction of Achilles tendinosis in rats. We used twelve
male Wistar rats randomly divided into experimental group and control group. The
experimental group ran on a treadmill for rats with acclivity of 10 degrees, five times per
week, daily during 70 minutes at a speed of 18 m / min for a period of 12 weeks. After the
protocol, the animals were sacrificad and Achilles tendons were removed left and right, fixed
and processed. We have twelve tendons in each group (n=12). After preparing slides using
stains of Hematoxylin-Eosin and Picrosirius Red, histomorphometric analysis was performed
of the same. The cell density increased in the experimental group compared to control, 626.08
 18.37 versus 445.08  16.48, 615.67 in field 1 and  13.51 versus 460.00  27.49, field 2,
with p < 0.05 . There was a greater deposition of type III collagen in the experimental groups
(50, 55 Â 4.002 versus 9.59 Â 0.435 with p < 0.001). It can be observed that the proposed
model is effective to develop a degenerative tendinosis type Achilles tendon injury.As doenÃas do tendÃo sÃo importantes causas de lesÃo em indivÃduos submetidos a treinamento excessivo. A tendinose à uma dessas lesÃes e caracteriza-se histologicamente pela hipercelularidade e aumento do colÃgeno tipo III. Diversos sÃo os modelos possÃveis de induÃÃo de tendinose em animais, sendo o mecÃnico o mais eficaz na reproduÃÃo dos eventos envolvidos nesse processo. Devido a caracterÃstica propulsora das patas traseiras, à necessÃrio um aclive de 10o na esteira utilizada na induÃÃo. A velocidade de corrida a qual foram submetidas as cobaias em modelos anteriores à um fator de difÃcil reproduÃÃo. HÃ, portanto, a necessidade da padronizaÃÃo de um mÃtodo mais reprodutÃvel. A pesquisa tem como objetivo definir e validar um modelo de induÃÃo de tendinose de Aquiles em ratos. Foram utilizados doze ratos machos Wistar divididos aleatoriamente em Grupo experimental e Grupo controle. O grupo experimental correu em uma esteira para ratos com aclive de 10o , cinco vezes por semana, durante 70 minutos diÃrios, com velocidade de 18 m/min, por um periodo de 12 semanas. ApÃs o protocolo, os animais foram submetidos à eutanÃsia e os tendÃes de Aquiles direito e esquerdo foram removidos, fixados e processados, tendo o grupo experimental 12 tendÃes (n=12) e o grupo controle 12 tendÃes (n=12). ApÃs a preparaÃÃo de lÃminas utilizando as coloraÃÃes de Hematoxilina-Eosina, Picrosirius Red, foi realizada a anÃlise histomorfomÃtrica das mesmas. A densidade celular aumentou no grupo experimental em relaÃÃo ao controle, 626,08  18,37 versus 445,08  16,48, no campo 1 e 615,67  13,51 versus 460,00  27,49, no campo 2, com p < 0,05. Houve maior deposiÃÃo de colÃgeno tipo III nos grupos experimentais (50, 55  4,002 versus 9,59  0,435 com p<0.001). Pode-se observar que o modelo proposto à eficaz para desenvolver uma lesÃo degenerativa tipo tendinose no tendÃo de Aquiles.
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Schlecht, Stephen Harold. "A Histomorphometric Analysis of Muscular Insertion Regions: Understanding Enthesis Etiology." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1328985192.
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Downer, Ann Nicole. "Molecular and Histomorphometric Changes of Buccal Alveolar Bone after Tooth Extraction." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1427300855.
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Penzias, Gregory. "Identifying the Histomorphometric Basis of Predictive Radiomic Markers for Characterization of Prostate Cancer." Case Western Reserve University School of Graduate Studies / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=case1473415195867117.
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Ma, Bingkui. "Growth modification of the temporomandibular joint by functional appliances : a histomorphometric study using sheep." Title page, contents and summary only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phm1112.pdf.
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Bibliography: leaves 234-245. In order to investigate growth modifications of the temporomandibular joint (TMJ) during dentofacial orthopaedic treatment, various functional appliances have been used to prompt the mandible into a protrusive position in various animal experimental models. The general purpose of this project was (i) to test the effectiveness of a functional appliance specially designed for sheep; (ii) to clarify whether or not forward mandibular displacement in sheep is associated with faster and/or redirected condylar growth; (iii) to evaluate the sheep as a model for dentofacial orthopaedic research by comparing the similarities of mandibular condylar growth in sheep and humans; (iv) to detail the position of the mandible during forward mandibular posturing and the effects of mandibular forward displacement on modelling and remodelling of the mandibular condyle.
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Cosgriff-Hernandez, Meghan-Tomasita JuRi. "Histomorphometric Estimation of Age at Death Using the Femoral Cortex: A Modification of Established Methods." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1338361172.
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Ingvoldstad, Megan E. "Femoral Midshaft Histomorphometric Patterning: Improving Microscopic Age at Death Estimates from Adult Human Skeletal Remains." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1353013643.
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Pinto, Deborrah C. "Variability in the Spine: A Histomorphometric Analysis of Spinous Processes from the Posterior Vertebral Arch." Columbus, Ohio : Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1238092506.
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Pazos, López Marta. "Rat optic nerve head anatomy within three- dimensional histomorphometric reconstructions of normal and early experimental glaucoma eyes." Doctoral thesis, Universitat Autònoma de Barcelona, 2016. http://hdl.handle.net/10803/381080.
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Objetciu: Reconstruir en 3D caps del nervi òptic (CNOs) de rata camb glaucoma experimental
(GExp) precoç unilateral, per proporcionar la primera descripció histomorfomètrica del CNO de
controls normales i amb GExp primerenc en aquesta espècie.
Métodos: Es va injectar sèrum salí hipertònic unilateralment en les venes epiesclerals de 8 rates
noruegues que van ser sacrificades 4 setmantes després (fixació-perfusió). Cinc observadors
enmascarats graduaren el dany de seccions del nervi òptic (NO) orbitari (1 Normal-5 Extens). Es
van reconstruir en 3D, es van visualitzar, deliniar i parametritzar els CNOs i l’esclera peripapil·lar.
Els paràmetres globals i específics per ull van ser avaluats conjuntament i regionalment amb un
model d’efectes linials mixtes amb criteris de significació ajustats per a comparacións múltiples.
Resultats: El CNO de la rata consisteix en 2 obertures esclerals (una superior neurovascular i una
altra otra inferior arterial) separades por una cingla escleral. Dins l’obertura superior, el nervi es
recolza un una prominent extensió de la Membrana de Bruch’s (MB) superiorment i està rodejat
d’un plexe vascular. El NO i l’Obertura Anterior del Canal Escleral (ASCO) van expandir-se
significativament en 7 dels 8 ulls amb GExp. En almenys 5 ulls amb GExp van detectar-se
expansions significativae de l’Obertura de la MB (BMO) (3-10%), de l’ASCO y de l’Obertura
Posterior del Canal Escleral (PSCO) (8-21% i 8-41% respectivament). L’expansió del NO va
correlacionar-se significativament amb el grau de dany (R2=0.668, p<0.05).
Conclusions: La complexitat 3D del CNO de rata y el gau en el què es diferencia de la del primat
han estat infravalorats en estudis previs en 2D. En el CNO de rata, el NO, el BMO que el rodeja I
el canal neurovascular s’expandeixen de manera precoç en resposta a l’elevació experimental crònica
de PIO. Aquestes troballes aporten nous punts de referència i objectius d’imatge per detectar el
desenvolupament de la neuropatia glaucomatosa experimental en ulls de rata.Objetivo: Reconstruir en 3D cabezas del nervio óptico (CNOs) de rata con glaucoma experimental (GExp) precoz unilateral, para proporcionar la primera descripción histomorfométrica de la CNO de controles normales y GExp precoz en esta especie. Métodos: Se inyectó suero salino hipertónico unilaterlamente en las venas epiesclerales de 8 ratas noruegas que se sacrificaron 4 semanas después (fijación-perfusión). Cinco observadores enmascarados graduaron el daño de secciones del nervio óptico (NO) orbitario (1 Normal-5 Extenso). Se reconstruyeron en 3D, se visualizaron, delinearon y parametrizaron las CNOs junto con la esclera peripapilar. Los parámetros globales y específicos por ojo fueron evaluados conjuntamente y regionalmente con un modelo de efectos lineares mixtos con criterios de significación ajustados para comparaciones múltiples. Resultados: La CNO de la rata consiste en 2 aperturas esclerales (una superior neurovascular y otra inferior arterial) separados por una cincha escleral. Dentro de la apertura superior, el nervio se apoya en una prominente extensión de la Membrana de Bruch’s (MB) superiormente y está rodeado de un plexo vascular. El NO y la Apertura Anterior del Canal Escleral (ASCO) se expandieron significativamente en 7 de 8 ojos con GExp. En al menos 5 ojos con GExp se detectaron expansiones significativas de la apertura de la MB (BMO) (3-10%), de la ASCO y de la Apertura Posterior del Canal Escleral (PSCO) (8-21% y 8-41% resp.). La expansión del NO se correlacionó significativamente con el grado de daño (R2=0.668, p<0.05). Conclusiones: La complejidad 3D de la CNO de rata y el grado en el que se diferencia de la del primate han sido infravalorados en los estudios previos en 2D. En la CNO de rata, el NO, el BMO que lo rodea y el canal neurovascular se expanden de manera temprana en respuesta a la elevación experimental crónica de PIO. Estos hallazgos proporcionan nuevos puntos de referencia y objetivos de imagen para detectar el desarrollo de la neuropatía glaucomatosa experimental en ojos de rata.
Purpose: To Three-Dimensionally (3D) reconstruct rat optic nerve heads (ONHs) with varying stages of unilateral early experimental glaucoma (EG), so as to provide the first histomorphometrical description of nomal controls and early EG ONH anatomy in this species. Methods: Hypertonic saline was unilaterally injected into the episcleral veins of 8 Brown Norway rats and animals were sacrificed 4 weeks later by perfusion fixation. Orbital optic nerve (ON) crosssections from were graded (1 normal, 5 extensive injury) by 5 masked observers. ONH’s with peripapillary ONH sclera were 3D reconstructed, visualized, delineated, and parameterized. Overall and animal-especific EG versus control eye ONH parameters differences were assessed globally and regionally by linear mixed effect models with significance criteria adjusted for multiple comparisons. Results: The rat ONH consists of 2 scleral openings (a superior neurovascular and inferior arterial) separated by a scleral sling. Within the superior opening, the nerve abuts a prominent extension of Bruch’s Membrane (BM) superiorly and is surrounded by a vascular plexus. Expansions of the ON and anterior scleral canal opening (ASCO) achieved statistical significance overall and in 7 of 8 EG eyes. In at least 5 EG eyes, significant expansions in Bruch’s Membrane Opening (BMO) (3-10%), the ASCO and Posterior Scleral Canal Openings (PSCOs) (8-21% and 8-41%, respectively) were detected. ON expansion was significantly correlated to ON damage (R2=0.668, p<0.05). Conclusions: The 3D complexity of the rat ONH and the extent to which it differs from the primate have been under-appreciated within previous 2D studies. In the rat ONH, the ON ansd surrounding BMO and neurovascular canal expand early in their response to chronic experimental IOP elevation. These findings provide phenotypic landmarks and imaging targets for detecting the development of EG optic neuropathy in the rat eye.
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Reis, Luana Marotta. "Influência do gel de edta a 24% no tratamento da doença periodontal induzida em ratos : análise histológica /." São José dos Campos : [s.n.], 2002. http://hdl.handle.net/11449/87960.
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Orientador: Yasmin Rodarte CarvalhoBanca: Yasmin Rodarte Carvalho
Banca: Mario taba Junior
Banca: Fernando Renó de Lima
Resumo: O objetivo deste estudo foi investigar se o gel de EDTA a 24%, (pH 7,0) apresenta efeito adjuvante ao tratamento periodontal não cirúrgico em periodontite induzida em ratos. Foram utilizados quarenta e cinco ratos machos, nos quais foi induzida a doença periodontal no 2° molar superior esquerdo por meio da inserção de ligadura de fio de algodão. Após 5 semanas, a ligadura foi removida e os animais submetidos à raspagem e aplainamento radicular. Os animais foram divididos aleatoriamente em 3 grupos: grupo EDTA (E), controle (C) e placebo (P); o grupo E foi condicionado com gel de EDTA a 24%, pH 7,0 durante 2 minutos, os animais do grupo C receberam apenas o tratamento mecânico e o grupo P foi condicionado com gel placebo, pH 7,0 durante 2 minutos. Todos os grupos foram irrigados com cloreto de sódio a 0,9% por 1 minuto. Em seguida, foi realizada marcação radicular na porção mais profunda da bolsa. Os animais foram sacrificados nos períodos de 4, 10 e 28 dias depois do tratamento. Os fragmentos da maxila contendo os molares foram submetidos à análise histológica e histomorfométrica para avaliação do grau de preenchimento e do tipo de tecido presente na região da marcação. As médias obtidas foram avaliadas pelo teste estatístico ANOVA e Teste de Tukey ao nível de significância p < 0,05. Os resultados mostraram que quanto ao tratamento realizado, não houve diferença estatisticamente significante com relação à reparação do periodonto. No entanto, o maior intervalo entre tratamento e tempo de sacrifício melhorou o reparo tecidual, uma vez que os animais sacrificados no período de 28 dias apresentaram menor quantidade de epitélio, maior quantidade de tecido conjuntivo sadio e preenchimento total da marcação. Foi verificada também ausência de tecido conjuntivo inflamado neste período. Concluiu-se que o gel de EDTA a 24%, pH 7,0 não contribuiu...(Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The aim of this study was to investigate if 24% EDTA gel (pH 7.0) presented adjunctive effect to non-surgical periodontal treatment in induced periodontitis in rats. Periodontitis was induced in the second upper left permanent molars of 45 male rats by means of ligature insertion of cotton floss. After 5 weeks, the ligature was removed and the animals underwent scaling and root planning. The animals were randomly divided in 3 groups: group E (EDTA), group C (control), and group P (placebo). Group E was conditioned with 24% EDTA gel (pH 7.0) for 2 minutes; the animals in group C received only mechanical treatment; and group P was conditioned with placebo gel (pH 7.0) for 2 minutes. All the groups were rinsed with 0.9% saline solution for 1 minute. Following that, root notching was carried out in the deepest part of the pocket. The animals were sacrificed at 4, 10, and 28 days after treatment. Jaw fragments containing the molars underwent histological and histomorphometric analysis for the evaluation of the degree of filling and the kind of tissue present in the notching region. The averages obtained were evaluated by means of ANOVA and Tukey statistical tests to a significance level of p < 0.05. With respect to the type of treatment employed, the results indicated that there were no statistically significant differences regarding periodontal repair. However, the longer the time between treatment and sacrifice, the better the tissue repair was, since the animals sacrificed at 28 days presented smaller quantities of epithelium, greater quantities of healthy connective tissue and total notch filling. Lack of inflamed connective tissue in this period was also observed. It was concluded that 24% EDTA gel (pH 7.0) did not contribute to periodontal tissue repair when used in combination with non-surgical periodontal treatment.
Mestre
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Önsten, Ingemar. "Fixation of total hip components in rheumatoid arthritis and srthrosis a radiographic, roentgen stereophotogrammetric, densitometric and histomorphometric study /." Lund : Dept. of Orthopaedics, University of Lund, Malmö General Hospital, 1994. http://books.google.com/books?id=mGVsAAAAMAAJ.
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Kuliwaba, Julia Suzanne. "Gene expression, bone remodelling, and microdamage in the human proximal femur: a molecular histomorphometric analysis of osteoarthritic bone." Title page, contents and summary only, 2003. http://web4.library.adelaide.edu.au/theses/09PH/09phk956.pdf.
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Moore, Alison Jane. "Quantitative histomorphometric analysis of the bone growth plate in infancy : a comparative study between SIDS and normal subjects /." Title page, contents and abstract only, 1998. http://web4.library.adelaide.edu.au/theses/09MSM/09msmm821.pdf.
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Reis, Luana Marotta [UNESP]. "Influência do gel de edta a 24% no tratamento da doença periodontal induzida em ratos: análise histológica." Universidade Estadual Paulista (UNESP), 2002. http://hdl.handle.net/11449/87960.
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reis_lm_me_sjc.pdf: 712396 bytes, checksum: 9f97f896a59d9ccc4cf4b02d5499632b (MD5)O objetivo deste estudo foi investigar se o gel de EDTA a 24%, (pH 7,0) apresenta efeito adjuvante ao tratamento periodontal não cirúrgico em periodontite induzida em ratos. Foram utilizados quarenta e cinco ratos machos, nos quais foi induzida a doença periodontal no 2° molar superior esquerdo por meio da inserção de ligadura de fio de algodão. Após 5 semanas, a ligadura foi removida e os animais submetidos à raspagem e aplainamento radicular. Os animais foram divididos aleatoriamente em 3 grupos: grupo EDTA (E), controle (C) e placebo (P); o grupo E foi condicionado com gel de EDTA a 24%, pH 7,0 durante 2 minutos, os animais do grupo C receberam apenas o tratamento mecânico e o grupo P foi condicionado com gel placebo, pH 7,0 durante 2 minutos. Todos os grupos foram irrigados com cloreto de sódio a 0,9% por 1 minuto. Em seguida, foi realizada marcação radicular na porção mais profunda da bolsa. Os animais foram sacrificados nos períodos de 4, 10 e 28 dias depois do tratamento. Os fragmentos da maxila contendo os molares foram submetidos à análise histológica e histomorfométrica para avaliação do grau de preenchimento e do tipo de tecido presente na região da marcação. As médias obtidas foram avaliadas pelo teste estatístico ANOVA e Teste de Tukey ao nível de significância p < 0,05. Os resultados mostraram que quanto ao tratamento realizado, não houve diferença estatisticamente significante com relação à reparação do periodonto. No entanto, o maior intervalo entre tratamento e tempo de sacrifício melhorou o reparo tecidual, uma vez que os animais sacrificados no período de 28 dias apresentaram menor quantidade de epitélio, maior quantidade de tecido conjuntivo sadio e preenchimento total da marcação. Foi verificada também ausência de tecido conjuntivo inflamado neste período. Concluiu-se que o gel de EDTA a 24%, pH 7,0 não contribuiu...
The aim of this study was to investigate if 24% EDTA gel (pH 7.0) presented adjunctive effect to non-surgical periodontal treatment in induced periodontitis in rats. Periodontitis was induced in the second upper left permanent molars of 45 male rats by means of ligature insertion of cotton floss. After 5 weeks, the ligature was removed and the animals underwent scaling and root planning. The animals were randomly divided in 3 groups: group E (EDTA), group C (control), and group P (placebo). Group E was conditioned with 24% EDTA gel (pH 7.0) for 2 minutes; the animals in group C received only mechanical treatment; and group P was conditioned with placebo gel (pH 7.0) for 2 minutes. All the groups were rinsed with 0.9% saline solution for 1 minute. Following that, root notching was carried out in the deepest part of the pocket. The animals were sacrificed at 4, 10, and 28 days after treatment. Jaw fragments containing the molars underwent histological and histomorphometric analysis for the evaluation of the degree of filling and the kind of tissue present in the notching region. The averages obtained were evaluated by means of ANOVA and Tukey statistical tests to a significance level of p < 0.05. With respect to the type of treatment employed, the results indicated that there were no statistically significant differences regarding periodontal repair. However, the longer the time between treatment and sacrifice, the better the tissue repair was, since the animals sacrificed at 28 days presented smaller quantities of epithelium, greater quantities of healthy connective tissue and total notch filling. Lack of inflamed connective tissue in this period was also observed. It was concluded that 24% EDTA gel (pH 7.0) did not contribute to periodontal tissue repair when used in combination with non-surgical periodontal treatment.
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Robling, Alexander G. "Histomorphometric assessment of mechanical loading history from human skeletal remains : the relation between micromorphology and macromorphology at the femoral midshaft /." free to MU campus, to others for purchase, 1998. http://wwwlib.umi.com/cr/mo/fullcit?p9924920.
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Al-Jandan, Badr. "The efficacy of biodegradable mesh as a fixation device for support of autogenous onlay bone grafts : a radiographic and histomorphometric analysis." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=112373.
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Objective. The objective of this study is to test the efficacy of the biodegradable mesh as an alternative fixation device to the titanium mesh for support of onlay particulate, bone and cancellous, marrow (PBCM) grafts. Two parameters were evaluated: (1) Bone graft volume maintenance; and (2) Calcified tissue surface area and distribution. Methods. 12 New Zealand white rabbits were used and divided equally into two groups. In both groups, endochondral, cancellous bone graft was harvested from the anterior ilium. The bone graft was then compressed and placed as an onlay onto the lateral aspect of the mandible using two chambers for each animal. In the control group, the chambers were made of titanium, however in the test group the chambers were made of poly L-lactide, polyglycolide and trimethylene carbonate mesh (Inion GTR(TM) Finland), and thereafter the chambers were fixed to the mandible. The animals were then sacrificed after eight weeks postoperatively, and then Micro-CT imaging was performed for the entire sample in order to determine the total volume of calcified tissue present under each chamber. In addition, Histologic sections were obtained from each chamber, and thereafter stained using Toluidine blue and Von Kossa for the purpose of histomorphometric analysis in order to determine the calcified tissue surface area. Results. When comparing the data from both the titanium (Ti) and the biodegradable mesh (PLA) groups, Micro CT analysis showed no significant statistical difference (P-value = 0.546) with regard to the percentage of bone found under the chambers (Ti 15.0% and PLA 13.83%). Interestingly, neither did the histomorphometric analysis show any significant statistical deference (P-value = 0.8272) with regard to the percentage of calcified tissue surface area (Ti 16.86% and PLA 16.17%). Moreover, this calcified tissue was also found to be evenly distributed in both groups. Conclusion. Biodegradable mesh made of poly L-lactic and polyglycolic acid copolymers appears to be an appropriate alternative to the Ti mesh for support of PBCM bone grafts. However, further clinical trails should be conducted to confirm these findings.
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Brinkman, John J. "The use of the histomorphometric analysis of age as applied to the subadults of the Commissary site characterized by fragmented bone." Virtual Press, 1997. http://liblink.bsu.edu/uhtbin/catkey/1045639.
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The purpose of this study was to determine if the histomorphometric analysis of age could be used to determine the age at death of subadults from the Commissary Archaeological Site. The histomorphometric analysis was used because of the fragmented condition of the bones. Sections from the left femur were obtained and processed for light and electron microscopy. Using the regression formula of Ahlqvist and Damsten and Ubelaker, age was estimated from the percentage of Haversian canals per 1 mm unit of bone. Results show the subadult population ranged in age from 0.9- 16.7 years. The correlation between the thickness of compact bone, the midshaft width, and the marrow cavity width to that of the assigned age supports the validity of the regression formula. The study demonstrates that the histomorphometric analysis can be applied to estimate age in subadult skeletons. This study further provides the first scientific analysis of age for the subadult skeletons of the Commissary site, an Early Late Woodland Native American population.Department of Anthropology
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Tull, Lara L. "Measuring bone density at distances lateral to the bone-implant interface with various stages of loading a histomorphometric analysis in the baboon /." [Gainesville, Fla.] : University of Florida, 2003. http://purl.fcla.edu/fcla/etd/UFE0002541.
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Lin, Shenyu [Verfasser], and Riccardo [Akademischer Betreuer] Giunta. "Anatomical and histomorphometric observations on nerve transfer in the distal forearm for the reconstruction of hand function / Shenyu Lin. Betreuer: Riccardo Giunta." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2016. http://d-nb.info/1104128977/34.
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Huang, Fan [Verfasser]. "Traumatic brain injury and bone healing: histomorphometric analyses of bone formation in a wild-type and leptin-deficiency murine model / Fan Huang." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2020. http://d-nb.info/121807616X/34.
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Cavaliere, Gustavo Alexandre. "Parâmetros histomorfométricos do intestino delgado em frangos de corte alimentados com rações contendo diferentes fontes de sorgo e concentrações de tanino." Universidade Federal de São Carlos, 2012. https://repositorio.ufscar.br/handle/ufscar/7033.
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Sorghum is increasingly being used for animal feed replacing corn, due to their similar nutritional characteristics, but with a lower cost. However one factor that negatively influences the nutritional value of sorghum is the presence of tannin that is an anti-nutritional factor that affects the nutrients absorption by the animal. We evaluated the effect of the sorghum with high and low tannins concentrations and / or the addition of tannic acid in diets based on corn and soybean on the single jejunal (duodenum, jejunum and ileum) histomorphometric parameters in broilers. There were used 150 male chicks with 1 day old of Cobb lineage, they were created up to 42 days in cages, in a controlled environment and receiving isoproteic and isoenergetic diets. After the period, were collected fragments of the 3 single jejunal segments, where they were measured and analyzed through a microscope with a coupled camera, according with the established parameters (villus height, crypt depth, thickness of muscle layer and relationship between villus numbers and villus bifurcations numbers). The addition of tannin in diets caused histomorphometric modifications in all parameters, but they did not cause significant changes for affecting their productive performance, such as weight and carcass yield.
O sorgo vem sendo cada vez mais utilizado para alimentação animal em substituição ao milho, devido suas características nutricionais semelhantes, porém com um custo muito menor. No entanto um fator que influencia negativamente o valor nutricional do sorgo é a presença de tanino, um fator antinutricional que compromete a absorção de nutrientes pelo animal. Avaliou-se o efeito do sorgo com alto e baixo teor em taninos e/ou a adição do ácido tânico em dietas à base de milho e soja, sobre os parâmetros histomorfométricos do intestino delgado (duodeno, jejuno e íleo) em frangos de corte. Foram utilizados 150 pintos machos de 1 dia de idade da linhagem Cobb, criados até aos 42 dias de idade em gaiolas, em ambiente controlado e recebendo rações isoprotéicas e isoenergéticas. Após o período foram coletados fragmentos dos 3 segmentos do intestino delgado, onde foram analisados e mensurados através de um microscópio com câmera acoplada, de acordo com os parâmetros estabelecidos (altura de vilosidade, profundidade de cripta, espessura de camada muscular e relação entre números de vilosidades e números de bifurcações) . A adição de tanino nas dietas causou modificações histomorfométricas em todos os parâmetros avaliados, porém essas modificações não ocasionaram alterações significativas para que comprometessem o desempenho produtivo das aves, como peso e rendimento de carcaça.
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Oliveira, Filho Osvaldo Mendes de 1964. "Functional and histomorphometric evaluation of median nerve lesion in wistar rats treated with GM1 = Avaliação funcional e histomorfométrica da lesão de nervo mediano em ratos wistar tratados com GM1." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/313131.
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Orientador: William Dias BelangeroTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
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Resumo: O objetivo deste trabalho foi comparar através da avaliação funcional pelo grasping test e análise histomorfométrica o tratamento da lesão do nervo mediano em ratos de linhagem Wistar através da microneurorrafia tradicional com a microneurorrafia associada à administração do monossialogangliosídeo (GM1) e avaliar especificamente se o GM1 melhora a regeneração axonal do nervo mediano e a função da musculatura por ele inervado. Material e Método: Foram empregados 32 ratos machos de linhagem Wistar. Destes, foram selecionados aleatoriamente 10 animais, grupo 0, para obtenção da força de preensão média em ratos normais, antes do procedimento cirúrgico. Esses animais foram reintegrados aos grupos. Foram criados o grupo I, com 10 animais, em que foi feita ressecção de 5 mm do nervo mediano do membro anterior direito e não foi submetido a nenhum tratamento. Nos outros grupos foi produzida uma lesão transversa do nervo mediano proximalmente ao epicôndilo medial criando-se os grupos II, tratados com microneurorrafia epineural externa e o grupo III, tratado com a microneurorrafia epineural externa associada à administração intraperitoneal de GM1. A cirurgia foi realizada imediatamente após a lesão e a técnica utilizada foi a sutura término-terminal. Foi realizada análise funcional semanal durante seis semanas através do teste de preensão da musculatura flexora dos dedos, que é específico para avaliar a ação do nervo mediano. Após esse período, os animais foram submetidos a eutanásia. As porções proximal e distal dos nervos foram coradas com azul de toluidina a 1% e realizada a análise histológica. Pela análise morfométrica obteve-se o número e diâmetro dos axônios nos cotos proximais e distais, criando-se uma nova fórmula com inclusão tanto do número como do diâmetro dos axônios para a avaliação da regeneração nervosa. Resultados: Os valores médios da força de preensão exercida pelos ratos do grupo 0 foram comparados aos animais dos grupos II e III através da análise de variância (ANOVA one way). Para a comparação dos valores médios da força realizada pelos ratos do grupo II e III foi feito o teste de Wilkoxon. Do ponto de vista funcional, o grupo III imprimiu uma maior força média com erro menor que 5% e realizou o teste de preensão mais precocemente. O grupo tratado com o GM1 apresentou um número 28% maior de axônios regenerados no segmento distal, com padrão histológico mais organizado e homogêneo e uma diferença significativa no diâmetro médio dos mesmos. Conclusão: Pode-se afirmar com erro menor que 5% que os grupos II (microneurorrafia) e III (microneurorrafia e GM1) apresentaram diferenças em relação à recuperação funcional, tendo o grupo III reagido melhor ao teste de preensão. O padrão histológico do grupo III apresentou maior grau de mielinização, tendo-se observado maior diâmetro médio nos axônios dos cotos distais (p=0,0056). Há um significativo indicio (p=0,0536) de que a utilização do GM1 nas cirurgias dos nervos periféricos melhora o padrão de regeneração axonal. Palavras Chaves: GM1, nervo mediano, regeneração axonal, ratos Wistar, avaliação funcional, morfometria
Abstract: Summary OBJECTIVES: The objective of this study was to compare the treatment of nerve median injuries in Wistar rats submitted to traditional microneurorraphy with the treatment that combined microneurorraphy and monossialoganglioside (GM1) administration while also specifically evaluating if GM1 promotes an increase in median nerve axonal regeneration, thus improving the function of the muscles in its territory of innervation. This comparison was done through functional evaluation measured by the grasping test and histomorphometric analysis. MATERIAL AND METHODS: Experiments were performed in thirty-two Wistar rats. Among them, 10 were randomly selected (group 0) to determine the average grasping strength in normal rats. These animals were then reunited with the others. There were three groups: group I (control group), submitted to a 5 mm lesion in the median nerve of the right forelimb and no treatment. Group II, submitted to lesion of the median nerve proximal to the medial epicondyle, treated with external epineural microneurorraphy, and group III, submitted to the same lesion and treated with external epineural microneurorraphy associated with intraperitoneal administration of GM1. Surgery was undertaken immediately after the damage to the nerve and end-to-end suture was used. Functional analysis through the grasping test of the flexor muscles of the fingers was assessed weekly; this test is specific to evaluate the action of the median nerve. In this experimental model, the animal is lifted by the tail and is stimulated to grasp a bar with its paw; the bar is located on the top of a conventional digital balance. While grasping the bar with its paw, the rat continued to be held by the tail until it releases the bar and the number on the scale is registered. After the functional evaluation the animals were euthanized. The proximal and distal portions of the nerves were colored with 1% toluidine blue dye. After the histologic exam, morphometric analysis was done by counting the number and diameter of the axons in the proximal and distal stumps. A new formula was designed including the number and diameter of the axons to evaluate nerve regeneration. RESULTS: The mean values of grasping strength exerted by rats in group I (control), were compared with group II (only microneurorraphy) and group III (microneurorraphy and GM1) through the analysis of variance (ANOVA one way). To compare the mean values of the strength sustained by rats in groups II and III, the Wilkoxon test was applied. From the functional perspective, the group that received GM1 performed the grasping test earlier, exerting a greater mean strength (error inferior to 5%). The microscopic analysis demonstrated that the group treated with GM1 showed a higher number of regenerated axons better organized and homogenous. And also that this group had a slightly thicker myelin sheath. There was a significant difference in the mean diameter of the axons of the distal segment and a number 28% higher of regenerated axons in the group treated with GM1. CONCLUSIONS: The authors can state with error inferior to 5% that the groups II and III showed differences in relation to functional recovery, group III performing better when submitted to the grasping test. Histological pattern of the group that received GM1 showed a higher degree of myelination. It was observed a greater mean diameter in the axons of distal stumps (p=0,0056). There is a significant indication (p=0,0536) that the use of GM1 in peripheral nerve surgery improves the pattern of axonal regeneration. Key Words: GM1, median nerve, axonal regeneration, Wistar rats, functional evaluation, morphometry
Doutorado
Medicina Interna
Doutor em Ciências Médicas
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Barros, Raquel Rezende Martins de. "A influência da superfície bioativa de implante na osseointegração. Estudo comparativo em cães." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-27082010-140752/.
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Entre as diferentes propriedades de uma superfície capazes de influenciar a deposição óssea ao redor de implantes, suas composições químicas e bioquímicas podem interferir no processo de reconhecimento a partir do tecido ósseo circundante. O presente trabalho se propôs a investigar se a funcionalização de superfícies de implante poderia influenciar a deposição óssea ao redor de implantes em um modelo animal. Para tanto, quatro grupos experimentais com mesma topografia microtexturizada, porém variando quanto à adição ou não de uma concentração de peptídeo bioativo foram testados. Metodologicamente, os pré-molares mandibulares bilaterais de 8 cães foram extraídos e após 12 semanas, foram instalados 6 implantes em cada cão, constituindo uma amostra de 48 implantes. Durante o período cicatricial de 2 meses, uma marcação policromática fluorescente foi realizada com o intuito de investigar a dinâmica de remodelação óssea. Estes marcadores ósseos foram administrados no terceiro dia após a instalação dos implantes, bem como após 1, 2, 4 e 6 semanas. A análise histomorfométrica revelou que a superfície microtexturizada modificada pela adição de uma baixa concentração peptídica obteve maior densidade óssea adjacente (54,6 ± 16,6%) quando comparada aos outros grupos (microtexturizada + veículo de hidroxiapatita = 46,0 ± 21,0%, somente microtexturizada = 45,3 ± 11,3% e microtexturizada com adição de alta concentração peptídica = 40,7 ± 15,3%), no entanto estas diferenças numéricas não foram estatisticamente significantes (p>0,05). Adicionalmente, em relação à análise de fluorescência, a comparação entre grupos demonstrou uma diferença estatisticamente significante em favor da superfície composta pela baixa concentração do peptídeo bioativo na área adjacente aos implantes no período de 4 semanas (p<0,001). Pode-se concluir que a funcionalização da superfície de implantes pode interferir na aposição óssea, em particular na densidade óssea, ressaltando que diferentes concentrações peptídicas podem conduzir a diferentes resultados. Dentro do padrão de remodelação óssea observado entre superfícies microtexturizadas, sendo estas funcionalizadas ou não, aquelas com baixa concentração do peptídeo bioativo estudado favoreceram a formação óssea adjacente aos implantes quando comparadas às demais no período avaliado.Among the different surface properties that influence the bone apposition around implants, the chemical or biochemical composition may interfere in its acceptance by the surrounding bone. The aim of this study was to investigate if a biofunctionalization of implant surface influences the bone apposition in a dog model and to compare it with other surfaces, such as a microstructured created by the grit-blasting/acid-etching process. The mandibular bilateral premolars of 8 dogs were extracted and after 12 weeks each dog received 6 implants, totaling 48 implants in the experiment. All the 4 experimental groups had the same microrough topography with or without some biofunctionalization treatment. After histomorphometric analysis it was observed that the modified microstructured surface with a low concentration of the bioactive peptide provided a higher adjacent bone density (54.6%) when compared to the other groups (microstructured + HA coating = 46.0%, microstructured only = 45.3% and microstructured + high concentration of the bioactive peptide = 40.7%), but this difference was only numeric and not statistically significant. The fluorescence analysis showed that bone remodeling is an active process resulting from the alternation of resorptive and formative activities. A similar pattern of bone remodeling was observed among the microstructured surfaces, biofunctionalized or not; however the addition of an adhesive peptide in low concentration favored the bone formation adjacent to the implants when compared to the other surfaces during the period evaluated. In conclusion the biofunctionalization of the implant surface could interfere in the bone apposition around implants in particular in terms of bone density and different concentrations of bioactive peptide lead to different results.
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Balli, Markus. "Steroide, biochemische Knochenmarker, Knochendichte und Histomorphometrie bei männlichen Osteoporosepatienten." [S.l.] : [s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961564202.
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Nor, Faridah M. "A comparative microscopic study of human and non-human long bone histology." Thesis, University of Bradford, 2009. http://hdl.handle.net/10454/4463.
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Identification of human or nonhuman skeletal remains is important in assisting the police
and law enforcement officers for the investigation of forensic cases. Identification of bone
can be difficult, especially in fragmented remains. It has been reported that 25 to 30% of
medicolegal cases, which involved nonhuman skeletal remains have been mistaken for
human. In such cases, histomorphometric method was used to identify human and
nonhuman skeletal remains. However, literature has shown that histomorphometric data for
human and nonhuman bone were insufficient. Additionally, age estimation in bone may
help in the identification of human individual, which can be done by using a
histomorphometric method. Age estimation is based on bone remodeling process, where
microstructural parameters have strong correlations with age. Literature showed that age
estimation has been done on the American and European populations. However, little work
has been done in the Asian population. The aims of this project were thus, to identify
human and nonhuman bone, and to estimate age in human bones by using
histomorphometric analysis. In this project, 64 human bones and 65 animal bones were
collected from the mortuary of the Universiti Kebangsaan Malaysia Medical Centre and the
Zoos in Malaysia, respectively. A standard bone preparation was used to prepare human
and nonhuman bone thin sections for histomorphometric assessment. Assessments were
made on the microstructural parameters such as cortical thickness, medullary cavity
diameter, osteon count, osteon diameter, osteon area, osteon perimeter, Haversian canal
diameter, Haversian canal area, Haversian canal perimeter, and Haversian lamella count per
osteon by using image analysis, and viewed under a transmitted light microscope. The
microstructural measurements showed significant differences between human and
nonhuman samples. The discriminant functions showed correct classification rates for
81.4% of cases, and the accuracy of identification was 96.9% for human and 66.2% for
animal. Human age estimation showed a standard error of estimate of 10.41 years,
comparable with those in the literature. This study project offers distinct advantages over
currently available histomorphometric methods for human and nonhuman identification and
human age estimation. This will have significant implications in the assessment of
fragmentary skeletal and forensic population samples for identification purposes.
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Weber, Annick. "CT-Osteoabsorptiometrie und Histomorphometrie nach Patellarsehnenplastik des vorderen Kreuzbandes beim Schaf." Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-122133.
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Gómez, García-Donas Julia. "Age estimation on two Mediterranean samples using rib histomorphometry." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/31496.
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Estimation of age is a crucial step for the identification of unknown individuals. Age is commonly assessed through macroscopic analytical methods based on the gross-examination of age degenerative changes in the skeleton. The choice of the methods relies on the taphonomic condition of the human remains and/or the skeletal element that is available. In cases of very fragmented bones, microscopic techniques remain one of the few approaches to estimate age. Thus, many histological age estimation methods have been developed for different bones and on different samples in the last forty years. Numerous intrinsic and extrinsic factors influence bone remodelling rates and have shown to affect the accuracy of histological aging methods. The present study investigates rib thin-sections from two Mediterranean samples, aiming to explore the applicability and reliability of histological methods in estimating age within these samples. Standard ribs were obtained from males and females (N = 88, Mean age = 60, SD = 17.90) from two samples, Cretans (Greece) and Greek- Cypriots (Republic of Cyprus). The costal elements were processed histologically according to standard protocols and thirteen raw and composite histomorphometric parameters (frequency number of intact and fragmentary osteons, total osteons, osteon population densities - including OPD(I) and OPD(F) - cortical area, total area, endosteal area, relative cortical area, osteon area, osteon perimeter and osteon circularity) were assessed. Intra- and inter-observer errors were examined. Due to the fragmented nature of the costal elements, sampling error was calculated as a means to explore whether the histological variables vary among six different topographical locations along the rib length. A validation study was carried out by applying four existing histological age prediction equations on the entire dataset and on the sub-datasets (sex and samples separately) in order to verify whether population-specific formulae are required for the Mediterranean samples. The relationship between the histological variables and age, as well as sex and samples, was determined through several statistical tests. Lastly, simple and multiple regression analyses were performed testing all possible combinations of variables. The best models ii were finally selected according to prediction power and goodness of fit indicators. The results from intra- and inter-observer errors indicated that most of the histological parameters achieved high levels of repeatability. The preliminary outcome from the sampling error pilot study suggested low variability among the six thin-sections from each rib. According to the validation study, three of the four age prediction equations resulted in high underestimation of age, indicating that population-specific formulae are needed to provide more accurate age estimates. Most of the histological variables showed a statistically significant correlation with age with some differences observed by sex and by sample. Forty-one models were generated concluding that osteon densities along with rib and osteon measurements formulae produced the most accurate results. The best model generated from the entire dataset included OPD and osteon circularity with a standard error of the estimate of 10.45 years. When sex and samples were separated, the best model selected included OPD and osteon perimeter producing a standard error of the estimate of 8 years for Cypriots. This research demonstrates that quantitative bone histology is a feasible method to estimate age on the Mediterranean samples obtaining errors rates that are in accordance with macroscopic ageing techniques. Inter-population variation in remodeling rates is suggested; however, the inclusion of other bones presenting different remodelling dynamics (such as femora) is recommended to further explore this hypothesis. This study contributes to the creation of population-specific standards for Cretans and Cypriots.
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Nor, Faridah Mohd. "A comparative microscopic study of human and non-human long bone histology." Thesis, University of Bradford, 2009. http://hdl.handle.net/10454/4463.
Full textAbstract:
Identification of human or nonhuman skeletal remains is important in assisting the police and law enforcement officers for the investigation of forensic cases. Identification of bone can be difficult, especially in fragmented remains. It has been reported that 25 to 30% of medicolegal cases, which involved nonhuman skeletal remains have been mistaken for human. In such cases, histomorphometric method was used to identify human and nonhuman skeletal remains. However, literature has shown that histomorphometric data for human and nonhuman bone were insufficient. Additionally, age estimation in bone may help in the identification of human individual, which can be done by using a histomorphometric method. Age estimation is based on bone remodeling process, where microstructural parameters have strong correlations with age. Literature showed that age estimation has been done on the American and European populations. However, little work has been done in the Asian population. The aims of this project were thus, to identify human and nonhuman bone, and to estimate age in human bones by using histomorphometric analysis. In this project, 64 human bones and 65 animal bones were collected from the mortuary of the Universiti Kebangsaan Malaysia Medical Centre and the Zoos in Malaysia, respectively. A standard bone preparation was used to prepare human and nonhuman bone thin sections for histomorphometric assessment. Assessments were made on the microstructural parameters such as cortical thickness, medullary cavity diameter, osteon count, osteon diameter, osteon area, osteon perimeter, Haversian canal diameter, Haversian canal area, Haversian canal perimeter, and Haversian lamella count per osteon by using image analysis, and viewed under a transmitted light microscope. The microstructural measurements showed significant differences between human and nonhuman samples. The discriminant functions showed correct classification rates for 81.4% of cases, and the accuracy of identification was 96.9% for human and 66.2% for animal. Human age estimation showed a standard error of estimate of 10.41 years, comparable with those in the literature. This study project offers distinct advantages over currently available histomorphometric methods for human and nonhuman identification and human age estimation. This will have significant implications in the assessment of fragmentary skeletal and forensic population samples for identification purposes.
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Funk, Julia Franziska. "Die Wirkung von systemisch appliziertem rekombinantem speziesspezifischem Wachstumshormon auf den Knochenumbau am Achsenskelett." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2004. http://dx.doi.org/10.18452/15076.
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Die Auswirkung der systemischen Applikation von 100µg/kg/KG rekombinantem speziesspezifischem Wachstumshormon (GH) über 26 Tage auf den Knochenumbau des Yucatan-Minischweins wurde an Wirbelkörpern, Beckenkämmen und Rippen histomorphometrisch untersucht. Der Einfluss der Distraktionsosteogenese auf die Mineralappositionsrate wurde ermittelt. 24 Tiere wurden an der linken Tibia osteotomiert, ein Fixateur externe wurde zur Distraktionsosteogenese von 2mm/d angelegt. 12 Tiere erhielten Wachstumshormon, 12 Placebo. Eine polychrome Fluoreszenzmarkierung wurde i.v. appliziert. Post mortem wurden Schnitte und Dünnschliffe der genannten Knochen angefertigt. Mittels Bildanalysesystem erfolgte die histomorphometrische Auswertung anhand der Parameter Knochendichte, Porosität, Trabekeldicke, Trabekelumbauparameter und Mineralappositionsrate (MAR). Signifikante Veränderungen durch Wachstumshormongabe konnten gezeigt werden. Unterschiede zwischen den Lokalisationen traten auf. So war die Knochendichte der GH-Tiere an Wirbelkörpern und Rippen signifikant geringer als in der Placebogruppe, kein signifikanter Unterschied zeigte sich am Beckenkamm. An allen 3 Lokalisationen war in der Wachstumshormongruppe eine signifikant erhöhte kortikale Porosität zu finden. Nur am Wirbelkörper waren die trabekuläre Knochendichte und die Trabekeldicke bei den GH-Tieren signifikant geringer als bei den Kontrolltieren. Die Knochenresorption war in der GH-Gruppe am Beckenkamm signifikant erhöht. Die Formation war bei den GH-Tieren sowohl iliakal als auch vertebral signifikant erhöht. Insgesamt ergab sich damit ein signifikant verminderter Anteil an ruhender Oberfläche bei den GH-Tieren. In ihrer Ausprägung waren diese Parameter zwischen Wirbel und Beckenkamm signifikant verschieden. Die MAR war in der GH-Gruppe signifikant höher als in der Placebogruppe, außerdem war sie während der Distraktionsphase signifikant höher als während der Konsolidierungsphase. Vergleicht man die Parameter mit Angaben aus der Literatur, kann bestätigt werden, dass die hier verwendeten Messmethoden geeignet sind, und dass das Yucatan-Minischwein ein geeignetes Modell zur Klärung von Fragestellungen der Knochenbiologie auch im Hinblick auf den Vergleich zum Menschen darstellt.The effect of systemic application of 100 µg/kg recombinant species specific growth hormone (GH) on the bone remodelling of 24 Yucatan mini pigs for a duration of 26 days was evaluated on vertebral body, iliac crest, and rib. The influence of distraction osteogenesis on the mineral apposition rate (MAR) was examined. 24 animals were osteotomized at the left tibia and treated with an external fixator for distraction osteogenesis of 2 mm/d. 12 of them received GH, the others placebo. A sequential polychrome fluorescent labelling was applied. After sacrifice thin and ground sections were manufactured. Using an image analysis system, a histomorphometrical evaluation was performed by means of bone density, porosity, trabecular thickness, trabecular remodelling parameters, and mineral apposition rate (MAR). Significant changes after GH application were visible. Differences between the locations were found. The bone density of vertebrae and ribs of the treatment group was significantly lower than that of the placebo group, whereas no difference was seen in the iliac crest biopsies. All 3 locations showed a significantly higher cortical porosity in the GH group. The trabecular bone density and the trabecular thickness were significantly decreased only in the vertebral bodies of the treatment group. The bone resorption was significantly elevated in the iliac crests of the GH group. The bone formation was increased in the iliac crest as well as in the vertebrae of the GH treated animals. Therefore, the resting surface was significantly decreased in the GH group. The extent of these effects was significantly different between iliac crest and vertebral body. The MAR was significantly higher in the GH group and during distraction osteogenesis. Compared to results of other study groups these parameters validate the methods of measurement and show that the Yucatan mini pig is a suitable model for research concerning bone biology, especially concerning the comparison with human parameters.
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Patterson, Catherine Elizabeth. "Histomorphometry-based modeling and simulation of multiple myeloma bone disease." Diss., University of Iowa, 2016. https://ir.uiowa.edu/etd/3161.
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Multiple myeloma is a plasma cell cancer that affects the bones, immune system, and kidneys. In this thesis, we focus on the impact on the bone, specifically routine bone remodeling. The bone remodeling process is governed by chemical signaling between several cell populations. In multiple myeloma patients, this process is out of balance. Bone destruction outpaces bone replacement, leaving patients with bone lesions. We describe the cell-signaling network that regulates bone remodeling and explain how it is impacted by multiple myeloma. We then present a series of mathematical models describing the bone remodeling process. We lay a thorough mathematical foundation, starting with the derivation of Savageau's power law approximations. Next, we introduce a novel one-dimensional moving-boundary partial differential equation model of this biological system. Our model improves upon models from the literature by including new cell populations, specifically osteoclast precursors, stromal cells, and tumor cells. We also discuss the model's computational results and their significance. We then discuss image processing techniques that can be used on bone marrow biopsies to gather data on the growth of a multiple myeloma tumor. By analyzing these medical images, we can extract tumor cell counts. In particular, we give the results of such an analysis for one patient using color unmixing. Image processing techniques, such as the ones presented here, could be used for validation of the models we present. The long-term goal of this project is the creation of a diagnostic tool that will aid oncologists in selecting the best treatment plan for their patients with multiple myeloma.
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Chehade, Mellick Joseph. "Bone resonance analysis, histomorphometry and the mechanics of fracture healing." Title page, contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phc515.pdf.
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BLEUZEN, PASCAL. "Etude comparative de l'osteocalcinemie et des donnees histomorphometriques dans l'osteoporose primitive d'involution." Nantes, 1989. http://www.theses.fr/1989NANT024M.
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Brockmann, Judith. "Die Beeinflussung der Knochenregeneration durch Vascular endothelial growth factor Korrelation von Histomorphometrie und Radiomorphometrie /." [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=96740424X.
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Muchow, Sarah. "Vergleich zweier identischer Fixateure mit unterschiedlichen Schersteifigkeiten am Schafmodell Histologie und Histomorphometrie der Frakturheilung /." Berlin : Mensch-und-Buch-Verl, 2005. http://www.diss.fu-berlin.de/2006/214/index.html.
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Schumann, Maria-Rose [Verfasser]. "Einfluss lokal transplantierter mesenchymaler Stammzellen auf die Regeneration nach Skelettmuskeltrauma : Morphologie und Histomorphometrie / Maria-Rose Schumann." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2012. http://d-nb.info/1030057249/34.
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Fromont, Pascale. "Les consequences architecturales osseuses de la corticotherapie : evaluation histomorphometrique chez 30 patients asthmatiques." Angers, 1994. http://www.theses.fr/1994ANGE1056.
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Bail, Hermann Josef. "Die Anwendung von rekombinantem homologen Wachstumshormon bei der Distraktionsosteogenese." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2004. http://dx.doi.org/10.18452/13931.
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An Yucatan Micropig(R) wurde überprüft, ob die systemische Applikation von Wachstumshormon (GH) in der Lage ist, die Regeneratkonsolidierung bei der Distraktionsosteogenese signifikant zu beschleunigen. Es erfolgte die Entwicklung eines speziellen externen Fixateurs (Halbringf.) mit welchem biomechanische in-vivo Messungen durchgeführte werden konnten. Ebenso wurden serologische, sonographische, röntgenologische, computertomographische, histomorphometrische und post mortem biomechanische Untersuchungen entwickelt und durchgeführt. Die Applikation von rekombinantem spezies-spezifischen Wachstumshormon bei der Distraktionsosteogenese zeigte bei dem von uns gewählten Tiermodell eine signifikante Beschleunigung der Regeneratkonsolidierung. Die biomechanischen Messungen ergaben eine mehr als verdoppelte Steifigkeit und Festigkeit der distrahierten Tibiae nach nur 10 Tagen Konsolidierungszeit im Vergleich zur Kontrollgruppe. Die CT erbrachte für die mit GH behandelten Tiere einen signifikant erhöhten Mineralisierungsgrad im Regenerat, zum gleichen Ergebnis kam die digitale Lumineszenzradiographie und die sonographische Vermessung des Regeneratdurchbaus. Somit zeigten alle Meßmethoden eine deutliche Beschleunigung der Regeneratkonsolidierung durch systemisch appliziertes GH. In den serologischen Untersuchungen fand sich eine signifikante Korrelation zwischen dem Serumspiegel der b-ALP und dem des IGF-I. Dies ist ein Hinweis darauf, dass die Wirkung des Wachstumshormons auf das Regenerat durch seinen Mediator IGF-I bedingt ist.To investigate the effect of systemic growth hormone (GH) application experiments were performed in a micropig animal model. Systemic daily s.c. injection of species-specific recombinant GH was investigated in Yucatan micropigs to evaluate the effect on intramembranous bone formation in distraction osteogenesis. Quantitative computed tomography (qCT), quantitative ultrasound, digital luminescence radiography, biomechanical in-vivo and post mortem testing, serological investigations as well as histomorphometric analyses were performed to investigate differences in regenerate formation. Systemic GH administration significantly increased in the present animal model the torsional stability of the regenerate in-vivo as well as post mortem in comparison to the contralateral side. Also all additional methods showed an accelerated regenerate consolidation in case of GH application. Systemic Insulin-like growth factor -I levels correlated well with the bone specific alkaline phosphatase levels. This may indicate, that the effect of GH on the distraction osteogenesis is IGF-I mediated.
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Jacq, Frédéric. "Etude histologique et histomorphométrique des vaisseaux intra osseux dans l'ostéonécrose non traumatique de la tête fémorale : à propos de 18 cas comparés à 22 cas de coxarthrose ou de fracture du col fémoral." Bordeaux 2, 1993. http://www.theses.fr/1993BOR23077.
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Muchow, Sarah [Verfasser]. "Vergleich zweier identischer Fixateure mit unterschiedlichen Schersteifigkeiten am Schafmodell : Histologie und Histomorphometrie der Frakturheilung / vorgelegt von Sarah Muchow." Berlin : Mensch-und-Buch-Verl, 2005. http://d-nb.info/980805708/34.
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Manresa, Quirante Carolina. "Estudio de la correlación entre parámetros histomorfométricos y de valoración de la estabilidad de los implantes. Nuevo método de determinación del BIC basado en imágenes BS-SEM." Doctoral thesis, Universitat de Barcelona, 2014. http://hdl.handle.net/10803/145718.
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La presente tesis doctoral se enmarca en el campo de la implantología, dentro de un proyecto encaminado a mejorar la predictibilidad clínica de los implantes. Los objetivos del presente trabajo han sido: 1) establecer un procedimiento de análisis de imagines para medir el contacto hueso-implante (BIC, Bone-to-Implant Contact) mediante una aproximación sistemática, no-subjectiva basada, en imágenes de microscopía electrónica de barrido con electrones retrodispersados (BS-SEM), 2) estudiar la relación entre la Frecuencia de Resonancia (FR) y otros parámetros de estabilidad del implante, tales como el parámetro histomorfométrico BIC, y 3) clarificar la controvertida relación entre la FR y las mediciones histomorfométricas de BIC.
Un total de 36 implantes dentales (9 mm length, Ø 4.0 mm; Biohorizons, Implant Systems Inc., Birmingham, AL, USA) con superficie SBM (Soluble blasting Media, arenado conpartículas solubles) se implantaron en las mandíbulas de seis perros Beagle. Las mediciones de AFR (Análisis de Frecuencia de Resonancia) se llevaron a cabo con un aparato magnético Osstell Mentor en el momento de la instalación de los implantes y durante los tiempos monitorizados, a las 1, 2, 4, 6 y 8 semanas, antes de la retirada del implante. Los perros se sacrificaron y los implantes se retiraron en bloque después de 8, 6, 4, 2, 1 y 0 semanas, respectivamente. Se consideró un grupo como tiempo 0, inmediatamente después de la implantación. Las muestras se incrustaron en polímeros de metacrilato de metilo (Technovit) y se cortaron a lo largo de su eje más largo. La observación de las muestras se llevó a cabo mediante BS-SEM, adquiriendo 10-16 imágenes por muestra. El procesado de las imágenes y la determinación del BIC se llevaron a cabo usando el paquete de procesado de imágenes Fiji. Las imágenes se unieron unas con otras, se filtraron y se estableció un umbral para los niveles de grises máximos, con lo que se obtuvo una imagen binaria del implante completo, que finalmente se dilató y perfiló. La longitud de este perfil se midió como el máximo posible BIC. Las regiones de coincidencia entre esta línea y el hueso fueron consideradas como el BIC real. El BIC% a diferentes tiempos se comparó con los correspondientes valores del cociente de estabilidad del implante (ISQ, Implant Stability Quocient) de las mediciones de AFR.
El método propuesto para la determinación del BIC, basado en SEM, que posee mucha mayor resolución que el microscopio óptico, permitió la adquisición de imágenes altamente discriminativas con gran contraste entre el implante y el hueso. La elevada resolución y el gran contraste de las imágenes SEM proporcionaron resultados más precisos que los obtenidos por el método clásico. Además, la metodología de análisis de imágenes descrita en este estudio delinea con precisión y automáticamente el contorno del implante; lo que da lugar a mediciones sin “sesgo”. El promedio del porcentaje de BIC fue 35%, con un rango de 24,7 a 45,5%. Estos valores fueron similares a los resultados publicados en la bibliografía. No se obtuvo una correlación estadísticamente significativa entre los valores de BIC y ISQ (Osstell Mentor). La ausencia de correlación entre estos dos parámetros está en concordancia con varios estudios previos en humanos y animales de experimentación. La falta de correlación entre el BIC y el ISQ sugiere que el ISQ, tal como se determina por el AFR, no es capaz de identificar la relación entre la FR y los datos histomorfométricos.The aims of the present work was 1) to establish an image analysis procedure for measuring the bone-to-implant contact (BIC) by a systematic non-subjective approach based on backscattered scanning electron microscopy (BS-SEM), 2) to study the relationship between resonance frequency (RF) and other parameters of implant stability, such as the histomorphometrical bone-to-implant contact (BIC) parameter, and 3) to clarify the controversial relationship between RF and histomorphometrical BIC measurements. A total of 36 dental implants {9 mm length, Ø 4.0 mm with a SBM (Soluble Blasting Media) surface} were implanted in six beagle dog mandibles. RFA (Resonance Frequency Analysis) assessments were performed with a magnetic Osstel Mentor device at the time of implant installation, and during the monitoring period at weeks 1, 2, 4, 6 and 8, before implant retrieval. The dogs were sacrificed and the implants were removed in block after 8, 6, 4, 2, 1 and 0 weeks, respectively and then embedded in resin and cut along their long axis. Sample observation was performed by BS-SEM, acquiring 10 to 16 images per sample. Image processing and BIC determination were performed using the Fiji image processing package. The methodology of image analysis described in this study delineates precisely and automatically the contour of the implant, which results in non-biased measurements. The average percentage of BIC was 35%, ranging from 24.7 to 45.5%. These values were similar to the results documented in the literature for implants of similar roughness in animal models. No statistically significant correlation between BIC and ISQ values (Osstell Mentor) was identified. The lack of correlation between BIC and ISQ values suggests that ISQ as determined by RFA is not able to identify the relationship between RF and histomorphometrical data.
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Agnew, Amanda Marie. "Histomorphometry of the Elderly Rib: A methodological approach with implications for biomechanics, function, and fracture risk." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1305558389.
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Shank, Stephanie Brooke. "Bone Damage Associated with Orthodontic Miniscrew Implants." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1299685868.
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MAREK, GAFFET ANNE. "Dosage du fluor osseux chez l'insuffisant renal : correlation avec l'histomorphometrie a propos de 44 patients du centre hospitalier de sfax (tunisie)." Amiens, 1992. http://www.theses.fr/1992AMIEM027.
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Cohen-Solal, Temstet Martine. "Aspect histologique osseux de l'insuffisance renale avant dialyse et evolution sous traitement vitamino-calcique non hypercalcemiant." Amiens, 1989. http://www.theses.fr/1989AMIEM037.
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Niechoda, Beata School of Surgery UNSW. "In vivo bone formation using Adipose Derived Stromal Stem Cells. The histomorphometry of the ovine defect model." Awarded by:University of New South Wales. School of Surgery, 2006. http://handle.unsw.edu.au/1959.4/27217.
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The use of stem cells to augment the healing of bone defects represents an exciting new frontier in many surgical disciplines. This thesis verified the in vitro osteogenic capability of ovine Adipose Derived Stromal Stem Cells (ADSCs) and Bone Marrow Derived Stem Cells (BMSCs), examined the in vivo osteogenic potential of the ovine ADSCs population and compared it to the in vivo osteogenic potential of ovine BMSCs. The pilot study used both cell populations, expanded and osteogenically stimulated ex vivo and mixed with resorbable porous hydroxyapatite-calcium carbonate bone graft substitute (ProOsteon 200R, Interpore Cross International, Irvine, CA). This study demonstrated the preferred length of time in ex vivo osteogenic stimulation of the ADSCs and BMSCs populations to promote the bone formation in vivo to be 7 days. In the main ovine study which employed 48 wethers, ADSCs and BMSCs were expanded, stimulated osteogenically for 7 days, mixed with ProOsteon 200R and deposited in an autologous manner into a bilateral medial femoral condyle confined cancellous defect. In vivo performance of 7 treatment groups was examined: 1. ???Bone autograft/ADSCs???, 2. ???Bone autograft/BMSCs???, 3. ???ProOsteon 200R/ADSCs???, 4. ???ProOsteon 200R/BMSCs???, 5. ???Bone autograft???, 6. ???ProOsteon 200R??? and 7. ???Empty defect???. The time-points were: 2, 4, 8 and 12 weeks. The analysis of the harvested specimens used the following methods: computerized tomography, histological assessment, histomorphometry and immunohistochemistry. There was a progressive and time dependant increase in woven bone in the defects treated with ADSCs and BMSCs across all time points. The amount of woven bone in the defects treated with the combination of ADSCs and ProOsteon 200R was comparable with the defects treated with the combination of BMSCs and ProOsteon 200R. In addition, the combination of ADSCs or BMSCs and ProOsteon 200R demonstrated no more bone than ProOsteon 200R alone. However, the ???Autograft/BMSCs??? and ???Autograft/ADSCs??? groups demonstrated a remarkable increase in the amount of woven bone formed in the defects across all time points when compared with all other groups. In addition, the amounts of bone formed in the ???Autograft/BMSCs??? and the ???Autograft/ADSCs??? group were comparable across all timepoints. The results of these studies support the hypothesis that ovine ADSCs and BMSCs populations in combination with the bone autograft can increase the formation of woven bone in the autologous orthotopic environment in a comparable manner.
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Membre, Hervé. "Etude de l'osteogenese dans differentes conditions experimentales." Nancy 1, 1990. http://www.theses.fr/1990NAN19005.
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Peixoto, Magno Eric Barbosa. "Histomorphometry of the Nerve Terminations of the Lower Nasal Shells of Humans by Immunofluorescence and Confocal Laser Microscopy." Universidade Federal do CearÃ, 2017. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=19318.
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CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel SuperiorNasal obstruction is one of the main complaint of patients with rhinitis, which is frequently associated with increased nasal airway resistance caused by hypertrophic changes of the inferior turbinates, however, studies have demonstrated that the objective measurement of nasal airway resistance does not always correlate with the subjective perception of the degree of nasal obstruction. The inferior turbinates are elongated, paired structures situated at the lateral nasal wall and made of a central core of osseous skeleton and a mucosal layer on each side almost exclusively covered with a pseudostratified ciliated columnar epithelium, with a well-defined basement membrane zone and a thick lamina propria. They play an important function in nasal physiology through reflex responses. The sensory nerves monitor the conditions of the mucosal microenvironment and initiate protective mechanisms immediately via axon responses. These nerve endings also have an important role on the perception of nasal patency. Despite the importance of these structures, little is known about their morphology and distribution in the normal nasal mucosa of human inferior turbinates. In order to obtain morphological and distribution data of nerve endings in inferior turbinates, specimens obtained from six individuals (three men and three women) with ages ranging from 16 to 76 years were submitted to fluorescent antibody technique with the marker pan -axonal anti-protein gene product 9.5 and confocal laser scanning microscopy. Free nerve endings were identified, mainly in the superficial layers of the lamina propria, some very close to the basement membrane. No complex nerve endings of the corpuscular type were identified. There were no differences in the distribution of nerve endings when compared to the septal and meatal faces or according to the gender of the individuals evaluated. Free nerve endings maintain an intimate relationship with seromucous glands and their ducts in the more superficial layers of the lamina propria, as well as with blood vessels, especially in their deeper portions, pointing to the role they play in the neuromodulation of glandular secretion and vasomotor control. Surgical techniques that allow the preservation of these free nerve endings may provide better postoperative outcomes, with a higher resolution of the clinical complaint of nasal obstruction and a lower rate of complications such as empty nose syndrome
ObstruÃÃo nasal à uma das principais queixas em pacientes com rinite, sendo frequentemente associada ao aumento da resistÃncia nasal pela hipertrofia das conchas nasais inferiores, contudo, estudos tÃm demonstrado que mensuraÃÃes objetivas da resistÃncia de via aÃrea nasal nem sempre se correlacionam à percepÃÃo subjetiva do grau de obstruÃÃo nasal. As conchas nasais inferiores sÃo estruturas alongadas, pareadas e situadas nas paredes nasais laterais, constituÃdas de um eixo Ãsseo central envolto, quase exclusivamente, por epitÃlio respiratÃrio pseudoestratificado cilÃndrico ciliado, com uma membrana basal bem definida e uma espessa lÃmina prÃpria. Possuem um papel importante na fisiologia nasal atravÃs de respostas reflexas. Os nervos sensoriais monitoram o microambiente da mucosa nasal e iniciam os mecanismos protetores imediatamente, via respostas axonais. Essas terminaÃÃes nervosas tambÃm possuem importante aÃÃo na percepÃÃo da patÃncia nasal. A despeito do importante papel dessas estruturas, pouco à conhecido acerca de sua morfologia e distribuiÃÃo na mucosa nasal normal de conchas inferiores de humanos. Com o objetivo de obter dados morfolÃgicos e de distribuiÃÃo das terminaÃÃes nervosas em conchas nasais inferiores, espÃcimes retirados de seis indivÃduos (trÃs homens e trÃs mulheres) com idades variando de 16 a 76 anos foram submetidos a estudo de imunofluorescÃncia com o marcador pan-axonal antiproduto gÃnico protÃico 9,5 e microscopia confocal de varredura a laser. Foram identificadas terminaÃÃes nervosas livres, principalmente, nas camadas mais superficiais da lÃmina prÃpria, algumas bem prÃximas à membrana basal. NÃo foram identificadas terminaÃÃes nervosas complexas, do tipo corpusculares. NÃo houve diferenÃas na distribuiÃÃo das terminaÃÃes nervosas quando comparadas as faces septal e meatal ou em funÃÃo do sexo dos indivÃduos avaliados. As terminaÃÃes nervosas livres mantÃm relaÃÃo Ãntima com glÃndulas seromucosas e seus ductos nas camadas mais superficiais da lÃmina prÃpria, bem como com vasos sanguÃneos, sobretudo, em suas porÃÃes mais profundas, apontando para o papel que possuem na neuromodulaÃÃo dos fenÃmenos de secreÃÃo glandular e controle vasomotor. TÃcnicas cirÃrgicas que permitam a preservaÃÃo dessas terminaÃÃes nervosas livres talvez possibilitem desfechos pÃs-operatÃrios melhores, com maior resoluÃÃo da queixa clÃnica de obstruÃÃo nasal e menor Ãndice de complicaÃÃes como a sÃndrome do nariz vazio (empty nose).
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Randall, Letitia E. "Bone Remodeling Surrounding Primary Teeth in the Maxilla and Mandible of Skeletally Immature Dogs." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1268087680.
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