Academic literature on the topic 'Hint Key Distribution'

Abstract Diatoms (Bacillariophyta), one of the most abundant and diverse groups of marine phytoplankton, respond rapidly to the supply of new nutrients, often out-competing other phytoplankton. Herein, we integrated analyses of the evolution, distribution, and expression modulation of two gene families involved in diatom nitrogen uptake (DiAMT1 and DiNRT2), in order to infer the main drivers of divergence in a key functional trait of phytoplankton. Our results suggest that major steps in the evolution of the two gene families reflected key events triggering diatom radiation and diversification. Their expression is modulated in the contemporary ocean by seawater temperature, nitrate, and iron concentrations. Moreover, the differences in diversity and expression of these gene families throughout the water column hint at a possible link with bacterial activity. This study represents a proof-of-concept of how a holistic approach may shed light on the functional biology of organisms in their natural environment.

Invertases are key enzymes in carbon partitioning in higher plants. They gain additional importance in the distribution of carbohydrates in the event of wounding or pathogen attack. Although many researchers have found an increase in invertase activity upon infection, only a few studies were able to determine whether the source of this activity was host or parasite. This article analyzes the role of invertases involved in the biotrophic interaction of the rust fungus Uromyces fabae and its host plant, Vicia faba. We have identified a fungal gene, Uf-INV1, with homology to invertases and assessed its contribution to pathogenesis. Expression analysis indicated that transcription began upon penetration of the fungus into the leaf, with high expression levels in haustoria. Heterologous expression of Uf-INV1 in Saccharomyces cerevisiae and Pichia pastoris allowed a biochemical characterization of the enzymatic activity associated with the secreted gene product INV1p. Expression analysis of the known vacuolar and cell-wall-bound invertase isoforms of V. faba indicated a decrease in the expression of a vacuolar invertase, whereas one cell-wall-associated invertase exhibited increased expression. These changes were not confined to the infected tissue, and effects also were observed in remote plant organs, such as roots. These findings hint at systemic effects of pathogen infection. Our results support the hypothesis that pathogen infection establishes new sinks which compete with physiological sink organs.

A new species Sybistroma genriki sp. n. from the Yunnan province of China is described. It belongs to S. incisa group of species, differing from other species in mostly yellow antenna with arista-like stylus much longer than postpedicel; postpedicel elongated, much longer than wide, acute apically; stylus apical, with dark and white apical swelling; lower postocular setae white; legs mostly yellow except mostly black mid and hind coxae and brown-black distal spot on hind femur. A key to 11 species from Sichuan and Yunnan is provided, based mainly on male secondary sexual characters. The known distribution of the dolichopodine genus Sybistroma Meigen, 1824, is briefly discussed.

Contrary to the typical southern distribution of the extant Kempyninae, a subfamily of Osmylidae, its fossil group shows the high diversification in the northern hemisphere during the Mesozoic. Herein a new genus with a new species, Mirokempynus profundobifurcus gen. et sp. nov., and a new species, Jurakempynus loculosus sp. nov., of Kempyninae, are described from the Middle Jurassic Jiulongshan Formation of Daohugou Village, Inner Mongolia, China. The new species share the characteristic synapomorphies of Kempyninae, e.g. subcostal veinlets mostly irregularly forked in the forewing and the distinctly expanded intramedial area with multiple rows of cells in the hind wing. The new genus distinctly shows a particular condition of intramedial area and MP branching in the hind wing. However, the condition of the region between MA and MP significantly broadened in the hind wing appear distinctively different to other known kempynine genera. A key is provided for all the genera of documented kempynines, both fossil and extant.

Purpose: Maximal oxygen uptake () is a key determinant of endurance performance. Therefore, devising high-intensity interval training (HIIT) that maximizes stress of the oxygen-transport and -utilization systems may be important to stimulate further adaptation in athletes. The authors compared physiological and perceptual responses elicited by work intervals matched for duration and mean power output but differing in power-output distribution. Methods: Fourteen cyclists ( 69.2 [6.6] mL·kg−1·min−1) completed 3 laboratory visits for a performance assessment and 2 HIIT sessions using either varied-intensity or constant-intensity work intervals. Results: Cyclists spent more time at during HIIT with varied-intensity work intervals (410 [207] vs 286 [162] s, P = .02), but there were no differences between sessions in heart-rate- or perceptual-based training-load metrics (all P ≥ .1). When considering individual work intervals, minute ventilation () was higher in the varied-intensity mode (F = 8.42, P = .01), but not respiratory frequency, tidal volume, blood lactate concentration [La], ratings of perceived exertion, or cadence (all F ≤ 3.50, ≥ .08). Absolute changes (Δ) between HIIT sessions were calculated per work interval, and Δ total oxygen uptake was moderately associated with (r = .36, P = .002). Conclusions: In comparison with an HIIT session with constant-intensity work intervals, well-trained cyclists sustain higher fractions of when work intervals involved power-output variations. This effect is partially mediated by an increased oxygen cost of hyperpnea and not associated with a higher [La], perceived exertion, or training-load metrics.

X-ray pair distribution function (PDF) analysis of Al0.57Sn0.43O1.71, a promising candidate as anode material for Li ion batteries, has been carried out using synchrotron radiation at 60 keV. The average and short-range ordering of nanocrystalline Al0.57Sn0.43O1.71was investigated both with the traditional Rietveld refinement method and with X-ray PDF analysis. The instrumental parameters of the high-resolution powder diffraction beamline P02.1, Petra III, have been characterized. Reverse Monte Carlo (RMC) refinements based on PDF data indicate that Al substitution induces local distortions around the Al/Sn atoms and that oxygen vacancies, induced by the Al substitution, are mostly located on an anion site around the Al atoms. Additionally, RMC refinements hint at a clustering of Al atoms.

Aulacofoenus Kieffer, which can be readily distinguished from other hyptiogastrine genera by having a short hidden ovipositor, the first flagellomere much longer than the second, a groove on the hind trochanter, and two discal cells in the fore wing, is revised. Fourteen species (10 new) are recognised; twelve of these [A. fallax (Schletterer), A. thoracicus (Guérin Menéville), comb. nov., A. bungeyi, sp. nov., A. fletcheri, sp. nov., A. goonooensis, sp. nov., A. kurmondi, sp. nov., A. loxleyi, sp. nov., A. marionae, sp. nov., A. perenjorii, sp. nov., A. swani, sp. nov., A. whiani, sp. nov., andA. wubinensis, sp. nov.] are recorded from Australia, and two [A. deletangi(Schletterer) and A. infumatus (Schletterer)] from South America. Two synonymies are proposed: A. asymmetricus (Turner) with A. thoracicus, comb. nov. and A. szépligetiiKieffer with A. deletangi. Relationships among genera of Hyptiogastrinae and species of Aulacofoenus are discussed, as is the restricted Gondwanic (amphinotic) distribution of the genus. A key to species is also presented.

Blazars are highly variable and display complex characteristics. A key characteristic is the flux probability distribution function or flux PDF whose shape depends upon the form of the underlying physical process driving variability. The BL Lacertae Mrk 421 is one of the brightest and most variable blazars across the electromagnetic spectrum. It has been reported to show hints of lognormality across the spectrum from radio to gamma-ray histograms of observed fluxes. This would imply that the underlying mechanisms may not conform to the “standard” additive, multi-zone picture, but could potentially have multiplicative processes. This is investigated by testing the observed lightcurves at different wavelengths with time-series simulations. We find that the simulations reveal a more complex scenario, than a single lognormal distribution explaining the multiwavelength lightcurves of Mrk 421.

Forty-one new species of the mostly neotropical genus Coniceromyia Borgmeier are described. The descriptions follow the methodology of recent works on the genus taxonomy and illustrate habitus, foremetatarsus, wing, hind femur, and hypopygium for each species. Unique features of some species are also illustrated, including several male features possibly related to sexual selection such as processes on different tarsomeres of the foreleg. New records for the known species are presented, as well as an identification key for the species of the genus and maps with their updated distribution. Coniceromyia brandaoi Ament & Amorim is synonymized with Coniceromyia plaumanni Borgmeier. Even though this work examined the Coniceromyia of the major collections of neotropical Phoridae, the high number of singletons and doubletons indicates that the real diversity of the genus may still be far from understood.

Abstract Every second more than 2 million new erythrocytes are released from the bone marrow of human adults, highlighting the tremendous turn-over of these cells. In parallel to hematopoietic stem cell niches, the last stages of erythropoiesis take place in specialized bone marrow niches, termed 'erythroid niches'. Concretely, the erythroid niche is composed of a 'central macrophage' which is surrounded by erythroid progenitor cells. Regardless of steady-state or stress erythropoiesis, iron availability is, beside erythropoietin, a key factor determining erythroid output and red blood cell quality, as reflected by hemoglobin content of these cells. It is well established that systemic iron availability for erythropoiesis, in the form of iron saturated transferrin (Tf), is mainly maintained via a recycling process of senescent red blood cells, which takes place in macrophages of the reticuloendothelial system. Yet, it is still a matter of debate if also central macrophages are involved in iron supply for red blood cell development in a more direct way due to their close proximity to developing red blood cells. Using a myeloid-specific knockout mouse strain, lacking the solely known iron exporter ferroportin (Fpn; Fpnfl/flLysMCre+/+ mice) and specific reporter mice (ROSA26tdTomatofl/fl Cx3cr1CreERT2 mice), we examined the connection between iron metabolism, erythropoiesis and central macrophages. Analysis of Fpnfl/flLysMCre+/+ animals at steady state revealed microcytic anemia, higher tissue iron loading, reduced hepatic hepcidin expression and distorted erythroid precursor population distribution in the bone marrow with no significant chances in Tf saturation (Tf-Sat). The latter is giving a first hint, that local bone marrow Fpn expression on macrophages may be important for iron supply for erythropoiesis. Strikingly, further work up via flow cytometry demonstrated that disturbances seen in bone marrow erythropoiesis were accompanied by nearby loss of resident bone marrow macrophages (defined as CD11blo, F4/80pos, MerTKpos). In parallel, a CD11bhi, F4/80pos, MerTKpos population came into existence, suggesting that these cells may compensate for the loss of 'canonical' central macrophages. Attempting to explain these intriguing results, we sought to investigate differentiation pathways and turnover of bone marrow central macrophages. First, we used the ROSA26tdTomatofl/fl Cx3cr1CreERT2 monocyte-specific reporter mice and techniques of transient monocyte labelling in utero and in adult phlebotomized animals to determine the origin of central macrophages. We could show that those cells undergo constant replenishment by circulating monocytes. Notably, the rate of this process got markedly increased upon recovery from blood loss and concomitant expansion of the central macrophage population. Second, by administration of a CCR2/CCR5 inhibitor (cenicriviroc), diminishing monocyte egress from the bone marrow and tissue infiltration, we could demonstrate decreased reticulocyte count during stress erythropoiesis, thus strengthening the direct impact of macrophages to support effective erythroid output. Next, effects of stress-induced erythropoiesis were investigated in Fpnfl/flLysMCre+/+ compared to Fpnfl/flLysMCre-/- mice. Amelioration of anemia after phlebotomy was extended, microcytosis was more pronounced and reticulocyte egress was diminished but prolonged. Of interest, Fpnfl/flLysMCre+/+ mice on a diet containing an 8-times higher iron content during phlebotomy, thus transiently increasing Tf-Sat, recovered from anemia wildtype-like. These results indicate that stress erythropoiesis with a high iron demand depends, under normal iron availability, in part on central macrophages and their nursing function to overcome the increased demand of iron. Ongoing experiments aim to identify how recruited bone marrow macrophages, i.e. central macrophages, contribute to erythropoiesis during stress - if central macrophages directly supply developing erythroid cells with iron in a Tf-free fashion or, if they are suppliers of additional growth factors that work synergistically with the Tf-bound iron to drive hemoglobin production. In summary our data clearly show that macrophages need to be recruited to the bone marrow for effective erythroid output during stress erythropoiesis. Disclosures Weiss: Kymab Ltd.: Consultancy. Theurl:Kymab Ltd.: Consultancy, Research Funding.

This thesis proposes a security solution in key management and Intrusion Detection System (IDS) for wireless sensor networks. It addresses challenges of designing in energy and security requirement. Since wireless communication consumes the most energy in sensor network, transmissions must be used efficiently. We propose Hint Key Distribution (HKD) for key management and Adaptive IDS for distributing activated IDS nodes and cooperative operation of these two protocols. HKD protocol focuses on the challenges of energy, computation and security. It uses a hint message and key chain to consume less energy while self-generating key can secure the secret key. It is a proposed solution to key distribution in sensor networks. Adaptive IDS uses threshold and voting algorithm to distribute IDS through the network. An elected node is activated IDS to monitor its network and neighbors. A threshold is used as a solution to reduce number of repeated activations of the same node. We attempt to distribute the energy use equally across the network. In a cooperative protocol, HKD and Adaptive IDS exchange information in order to adjust to the current situation. The level of alert controls the nature of the interaction between the two protocols.

The Victorian Mallee region encompasses the Little Desert, the Big Desert, the Sunset Country and the Hattah-Kulkyne. Each area is unique and with different topography, vegetation and fauna. The region experiences consistently higher temperatures, lower rainfall and contains a greater diversity of reptiles than any other part of the state. Snakes, Lizards and Frogs of the Victorian Mallee represents the first comprehensive publication on the herpetofauna of the region. It covers 56 species that inhabit the area as well as a further 24 species occurring in fringe riverine and woodland systems. The reader is able to identify species by means of a photograph supported by a distribution map, a diagnostic features key and descriptive species accounts. The book includes a discussion of venomous snakes, information on first aid for snakebites, and hints for snake prevention around the house.

The 20th anniversary of Economic and Monetary Union (EMU) offers an opportunity to look back on the record of the European Central Bank (ECB) and learn lessons that can improve the conduct of policy in the future. This volume charts the way the ECB has defined, interpreted, and applied its monetary policy framework—its strategy—over the years from its inception, in search of evidence and lessons that can inform those reflections. Our ‘Tale of Two Decades’ is largely a tale of ‘two regimes’: one—stretching slightly beyond the ECB’s mid-point—marked by decent growth in real incomes and a distribution of shocks to inflation almost universally to the upside; and the second—starting well into the post-Lehman period—characterized by endemic instability and crisis, with the distribution of shocks eventually switching from inflationary to continuously disinflationary. We show how the most defining feature of the ECB’s monetary policy framework, its characteristic definition of price stability with a hard 2 per cent ceiling, functioned as a key shock absorber in the relatively high-inflation years prior to the crisis, but offered a softer defence in the face of the disinflationary forces that hit the euro area in its aftermath. The imperative to halt persistent disinflation in the post-crisis era therefore called for a radical, unprecedented policy response, comprising negative policy rates, enhanced forms of forward guidance, a large asset purchase programme and targeted long-term loans to banks. We study the multidimensional interactions among these four instruments and quantify their impact on inflation and the macroeconomy.

Collaborative re-use of design and manufacturing data is one way that global e-commerce could significantly reduce development costs and lead times of new products. However the proliferation of web based catalogues for standard components (e.g. brochure-ware for nuts and washers etc.) only hints at the possible productivity gains if a 3D search engine could be created. Consequently the research reported here is motivated by the belief that shape matching technology is key to enabling a much deeper form of Internet-based collaborative commerce. This paper describes the coarse “shape” filters that support a 3D, Internet-based search engine, known as ShapeSifter, whose ultimate aim is to enable the location of parts already in production that have a shape similar to a desired “newly designed” part. The challenge of the research is to identify the key metrics that produce meaningful characterizations of 3D models for similarity comparison purposes. In this context the work reported focuses on the use of novel indices based on an object’s convex-hull and surface area distribution to carry out a coarse filtering of candidates prior to the application of more detailed analysis (e.g. the construction of multi-dimensional feature vectors). In doing this, the paper describes the crucial role played by a benchmark database of geometrical similar objects.

Rock drilling is one of the elementary processes in mining industry. As larger diameter holes are drilled by hitting with units attached to the crone adapter down in the hole, the smaller blast holes are hit with units impacting the rod neck at rock surface. Key question in the performance and energy efficiency of the blast-hole drilling process is then, how completely the impact energy can be transmitted to the crone over the relatively long wave guide. There have been discussions about the effect of wave length and shape on to the penetration dynamics of rock drills. As the drilling process is a continuous set of hits following each other at relatively high constant period and the response is a random overlapped mixture of coming waves and returning reflected waves from multiple delayed hits, a detailed analysis of penetration dynamics is rather a complex problem. To overcome this difficulty, a full-scale half-manual test-drill has been designed and built to produce single hits for a systematic production and analysis of optimally shaped stress waves. The test-drill is an air-powered pneumatic gun, whose impact energy can be adjusted by setting the initial pressure level to correspond the desired end velocity of the piston. The design parameters, by which the pulse shape can be modified, are the length and the geometric profile of the piston body. The first problem to be faced is then to determine the optimal pulse shape for maximized penetration depth and the second one is to produce such desired shape by an optimal choice of the design parameters. The rig has been modelled using finite elements for the rod system and adiabatic state equations for the compressed and expanding air volumes. By modifying the design parameters, different penetration responses can be produced. In the first step, the model has been updated by means of experimental response measurements. The second step has been to modify the geometrical profiles of the piston body by starting from piece-vice linear and parabolic cross-sectional distributions. The output of the numerical analysis is to evaluate the penetration depth pro hit for different geometrical profiles. The most promising geometry has then been selected for the fabrication of the prototype piston. An experimental hitting test then completes the analysis, whose repeatability showed to be limited due to the random variation of the rock properties in the test bed. Test results obtained by using more regular concrete specimens exhibited reduced deviations in the responses, but the weakness in the test is the different damaging mechanism during the penetration. Another option is the use of an artificial load-sensing endsupport in order to produce a known boundary condition to replace the tool-rock resistance in the model-updating phase.