Dissertations / Theses on the topic 'High content of protein'
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Leuchowius, Karl-Johan. "High Content Analysis of Proteins and Protein Interactions by Proximity Ligation." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-119530.
Full textTavares, Joana Formigal. "Identification of novel regulators of protein synthesis fidelity using high content genetic screens." Doctoral thesis, Universidade de Aveiro, 2018. http://hdl.handle.net/10773/22825.
Full textProtein synthesis is central to life and is being intensively studied at various levels. The exception is mRNA translational fidelity whose study has been hampered by technical difficulties in detecting amino acid misincorporations in proteins. Few genes have so far been associated to the control of protein synthesis fidelity and it is unclear how many genes control this biological process. We investigated the role of RNA modification by RNA modifying enzymes (RNAmods) in protein synthesis efficiency and accuracy. Our hypothesis was that RNAmods that modify tRNA nucleosides (tRNAmods) have a significant impact on protein synthesis through modulation of codonanticodon interactions. To address this issue, we focused our work on tRNAmods involved in the modification of tRNA anticodons. The biology of these enzymes is still poorly understood, but they are involved in RNA processing, stability and function and their deregulation is associated with cancer, neurodegenerative, metabolic and other diseases. We have set up a yeast genetic screen and used mass-spectrometry methods to determine the role of tRNAmods on proteome homeostasis. Our work identified a subgroup of yeast tRNAmods that play essential roles in protein synthesis fidelity and folding. The genes that encode insoluble proteins isolated from yeast cells lacking U34 modification were enriched in codon sites that are decoded by the hypomodified tRNAs. These aggregated proteins also participate in specific biological processes, suggesting that tRNAmods are linked to specific physiological pathways. Interestingly, we detected amino acid misincorporations at the codon sites decoded by the anticodons of the hypomodified tRNAs, demonstrating that tRNA U34 modifications control translational error rate.
A síntese proteica é central para a vida e tem sido extensivamente estudada a vários níveis. Contudo, o estudo da fidelidade da tradução do mRNA tem progredido lentamente devido a dificuldades técnicas na deteção de incorporações incorretas de aminoácidos nas proteínas. Poucos genes têm sido associados com o controlo da fidelidade da síntese proteica e não é evidente quais os genes que controlam este processo biológico. Nesta tese investigámos o papel da modificação dos nucleósidos do RNA na eficiência e precisão da síntese proteica. A nossa hipótese é que as enzimas que modificam nucleósidos do tRNA (tRNAmods) têm um impacto significativo na síntese proteica através da modulação das interações codão-anticodão. A biologia das tRNAmods e das modificações do tRNA são ainda pouco conhecidas, mas estão envolvidas na estabilidade e função do RNA e mutações nos seus genes causam doenças neurodegenerativas, metabólicas, cancro, entre outras. Neste projeto realizámos um rastreio genético em levedura com o objetivo de identificar tRNAmods que asseguram a homeostase do proteoma (proteostase) e usámos espectrometria de massa para clarificar o papel das tRNAmods na fidelidade da síntese proteica. Os resultados do estudo genético mostram que um sub-grupo de tRNAmods envolvidas na modificação de nucleósidos do anticodão do tRNA são essenciais para manter a estabilidade do proteoma. Outras tRNAmods estudadas não produziram impactos visíveis na proteostase. Os genes de proteínas agregadas que isolámos a partir de células de levedura com tRNAs hipomodificados são enriquecidos em codões descodificados por estes tRNAs. Os nossos dados mostram também que tais proteínas participam em processos biológicos específicos e têm níveis de aminoácidos errados mais elevados que as células wild-type. Estes dados mostram que certas modificações do tRNA são essenciais para a fisiologia celular, estabilidade do proteoma e fidelidade da síntese proteica.
Jüttemann, Thomas. "Adding 3D-structural context to protein-protein interaction data from high-throughput experiments." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/5666.
Full textKattah, Michael George. "High-content protein arrays for characterizing immune responses and pathophysiology at the molecular level /." May be available electronically:, 2008. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.
Full textSchmiele, Marcio 1979. "Interações físicas e químicas entre isolado protéico de soja e glúten vital durante a extrusão termoplástica a alta e baixa umidade para a obtenção de análogo de carne = Physical and chemical interactions between isolated soy protein and vital gluten during thermoplastic extrusion at high and low moisture content to obtain meat analogue." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/255892.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: Os análogos de carne obtidos por extrusão termoplástica de proteínas vegetais são caracterizados pelo seu elevado teor proteico e estrutura semelhante às fibras da carne, envolvendo diversos tipos de ligações e/ou interações químicas entre as proteínas. O objetivo deste trabalho foi avaliar as características tecnológicas e físico-químicas de análogos de carne, à base de isolado proteico de soja, obtidos por processo de extrusão termoplástica a alta umidade (AU) e baixa umidade (BU). Para cada condição de umidade foi utilizado um Delineamento Composto Central Rotacional de três variáveis independentes (glúten vital, umidade de condicionamento e temperatura de extrusão). As variáveis dependentes avaliadas foram a textura instrumental, cor instrumental, capacidade de absorção de água, índice de solubilidade em água, capacidade de absorção de óleo, índice de dispersibilidade de proteína, energia mecânica específica e o tipo de interações proteicas. Estas interações foram avaliadas através de sete tipos de solventes específicos: (i) tampão fosfato para as proteínas no estado nativo; (ii) dodecil sulfato de sódio para as interações hidrofóbicas e iônicas; (iii) Triton 100X para as interações hidrofóbicas; (iv) ureia para as interações hidrofóbicas e pontes de hidrogênio; (v) ß-mercaptoetanol para as ligações dissulfeto; e (vi) ß-mercaptoetanol e ureia e (vii) dodecil sulfato de sódio e ureia, para avaliar o efeito sinérgico entre os sistemas. O ponto otimizado (caracterizado principalmente por promover maiores valores de L* e de capacidade de absorção de água, menores valores de índice de solubilidade em água, de capacidade de absorção de óleo, de desnaturação proteica e valores intermediários de textura instrumental e de energia mecânica específica) foi processado juntamente com uma amostra controle para ambos os processos com o intuito de validar os modelos matemáticos e avaliar as possíveis alterações na morfologia dos análogos de carne, na massa molecular das proteínas, na composição de aminoácidos totais e na desnaturação proteica. As melhores condições de processamento foram obtidos para os análogos de carne contendo de 12 e 5 % de glúten vital, 58 e 18 % de umidade de condicionamento e 135 e 100 °C para a temperatura de extrusão, para o processo AU e BU, respectivamente. As principais interações proteína-proteína encontradas nos análogos de carne foram as ligações dissulfeto e ligações de hidrogênio para o processo AU e as ligações dissulfeto e interações iônicas para o processo BU. A adição de glúten vital promoveu uma aparência mais lisa e melhor orientação na estrutura das fibras. Verificou-se que ocorreu aumento nas proteínas de baixa massa molecular e diminuição nas proteínas de alta massa molecular. No perfil de aminoácidos totais houve maior variação negativa para os aminoácidos essenciais (triptofano e treonina), semi essenciais (cisteína) e não essenciais (serina), indicando que houve redução no valor nutricional. As estruturas secundárias (a-hélice, ß-folha, ß-volta e a estrutura desordenada) mostraram alteração na sua conformação devido à desnaturação proteica e formação de novos agregados
Abstract: Meat analogue obtained by termoplastic extrusion of vegetable proteins are characterized by its high protein levels and structure similar to meat fibers, which comprises many types of chemical bonds and/or interactions between proteins. The aim of this work was to evaluate the technological and physico-chemical characteristics of meat analogue based on isolated soy protein obtained by thermoplastic extrusion process at high moisture (HM) and low moisture (LM) content. For each moisture condition was used a Central Rotational Composite Design with three independent variables (vital gluten, moisture content and extrusion temperature). The dependent variables evaluated were instrumental texture, instrumental color, water absorption capacity, water solubility index, oil absorption capacity, protein dispersibility index, specific mechanical energy, and the type of protein interactions. These interactions were evaluated using seven specific solvents types: (i) phosphate buffer for proteins in native state; (ii) sodium dodecil sulphate for hydrophobic and ionic interactions; (iii) Triton 100X for hydrophobic interactions; (iv) urea for hydrophobic interactions and hydrogen bonds; (v) ß-mercaptoethanol for dissulfide bonds; and (vi) ß-mercaptoethanol and urea and (vii) sodium dodecil sulphate and urea, for the synergistic effect between the systems. The optimized point (characterized mainly by promoting higher values for L* and water absorption capacity, lower values for water solubility index, oil absoption capacity and protein denaturation and intermediate values for instrumental texture and specific mechanical energy) was processed, together with a control sample for each processes, in order to validate the mathematical models and to evaluate possibles changes in the meat analogues morphology, in the protein molecular weight, in the total amino acid composition, and in the protein denaturation. The best processing conditions were obtained for the meat analogue containing 12 and 5 % of vital gluten, 58 and 18 % of moisture content and 135 and 100 °C of extrusion temperature, for the HM and LM processes, respectively. The main protein-protein interactions found in meat analogues were the dissulfide bonds and hydrogen bonds for the LM process and the dissulfide bonds and ionic interactions for the HM process. The addition of vital gluten promoted a smoother appearance and better orientation in the fiber structure. It was found that occured an increase in the protein with low molecular weight and a reduction in the protein with high molecular weight. There were a greater negative variation for the essential (tryptophan and threonine), semi-essential (cysteine) and nonessential (serine) amino acids in the total amino acid profile, indicating a reduction of the nutritional value. The secondary structure (a-helix, ß-sheet, ß-turn and disordered structure) showed alteration in its conformation due to the protein denaturation and formation of new aggregates
Doutorado
Tecnologia de Alimentos
Doutor em Tecnologia de Alimentos
Tulukcuoglu, Güneri Ezgi. "Development of microfluidic device for high content analysis of circulating tumor cells." Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066583/document.
Full textMetastasis is the advanced stage of cancer progression and is the cause of 90% of deaths in cancer disease. During metastatic cascade, it is suggested that the successful metastatic initiation depends on the survival of circulating tumor cells (CTCs). CTCs are the cells that shed from the primary or secondary tumor sites into the blood circulation. it is now widely recognized as potential biomarker for companion diagnostics in which high number of CTCs in blood can indicate association with poor survival or high risk of disease progression. Besides, following the number of CTCs during the course of treatment can help to adapt the selected therapy and predict the treatment efficacy. On the other hand molecular characterization can provide patient stratification and identifying the therapeutic targets. However they are extremely rare in the bloodstream, estimated between 1-10 CTC among 6×106 leukocytes, 2×108 platelets and 4×109 erythrocytes per one mL of blood which makes their isolation very challenging. A very attractive way of isolation of CTCs is to integrate microfluidics. Microfluidics offers great advantages such as low volume of reagent consumption and short analysis times with automation as well as isolation and detection analysis can be integrated resulting in highly efficient biomedical devices for diagnostics. As parallel to state of the art, a powerful microfluidic device for circulating tumor cells capture and analysis had already been developed previously in our laboratory. The principle of capture is based on self-assembly of antibody-coated (EpCAM) magnetic beads in which the cells are enriched by EpCAM surface antigen which is found commonly in epithelial origin cancer cells. This system was already validated with cell lines and patients samples. However, the system did not allow isolation/detection of subpopulations of CTCs or performing high content molecular characterization. Therefore, my PhD project aimed at further improving the capabilities of the system on the main two aspects: targeting subpopulations of CTC and studying of protein interactions of CTCs in Ephesia System
Skutnik, Benjamin C. "The effects of high intensity interval training on resting mean arterial pressure and C-reactive protein content in prehypertensive subjects." Thesis, Kansas State University, 2013. http://hdl.handle.net/2097/15774.
Full textDepartment of Kinesiology
Craig A. Harms
Subjects with prehypertension are at risk for developing hypertension (HTN). Hypertension is associated with low-grade systemic inflammation (LGSI). Aerobic exercise training (ET) is a proven means to reduce both blood pressure and LGSI in healthy and diseased subjects. Recently, high intensity interval training (HIIT) has been show to elicit similar cardiovascular and metabolic adaptations as ET in healthy and at-risk populations in a more time efficient manner. Therefore, we hypothesized that HIIT would elicit greater reductions in blood pressure and LGSI than ET. Twelve pre-hypertensive subjects (systolic blood pressure 127.0 ± 8.5 mmHg; diastolic blood pressure 86.2 ± 4.1 mmHg) were randomly assigned to an ET group (n=5) and a HIIT group (n=7). All subjects performed an incremental test to exhaustion (VO2max) on a cycle ergometer prior to, after 4 weeks, and after 8 weeks of training. Resting heart rate and blood pressure were measured prior to and three times a week during training. LGSI was measured via high-sensitivity C-reactive protein (hs-CRP) prior to, after 4 weeks and after 8 weeks of training. ET subjects performed an eight week exercise training program at 40% VO2 reserve determined from the VO2max test, while HIIT subjects performed exercise at 60% peak power determined from the VO2max test. ET group trained four days/week while HIIT trained three days/week. ET exercised for 30 minutes continuously at a constant workload and cadence of 60 rpm while HIIT performed a protocol on a 1:1 work-to-rest ratio at a constant workload and cadence of 100 rpm. Both groups showed similar (p<0.05) decreases in mean arterial (ET = -7.3%, HIIT = -4.5%), systolic (ET = -6.6%, HIIT = -8.8%), and diastolic (ET= -9.7, HIIT= -8.2%) blood pressure. HIIT decreased in LGSI (-33.7%) while ET did not change LGSI (p>0.05). VO2max increased ~25% with both HIIT and ET with no differences (p>0.05) between groups. These data suggest both HIIT and ET similarly decreased resting blood pressure and increased VO2max while HIIT was effective in decreasing LGSI in subjects who were pre-hypertensive.
Kapor-Drezgic, Jovana. "High glucose alters mesangial cell protein kinase C activity and isoform cellular content and localization, role of the polyol pathway." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0003/MQ40808.pdf.
Full textZhu, Seng. "Study of the mechanism of Tunneling nanotubes formation and their role in aggregate proteins transfer between cells." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS377.
Full textTunneling nanotubes are actin-based cell protrusions that mediate cell-to-cell communication by transferring cellular cargos. The different types of intercellular communication are increasing by being considered as potential targets for the treatment of various diseases, such as infectious diseases linked to viruses and bacteria, cancers or neurodegenerative diseases. Recent studies have highlighted a prion-like mechanism of propagation of protein misfolding in a variety of common, non-infectious, neurodegenerative diseases such as Alzheimer’s disease (AD), Frontotemporal dementia (FTD), Parkinson’s disease (PD), and Polyglutamine (PolyQ) diseases, which are characterized by the accumulation of misfolded proteins in the brain of patients. Thus, new therapeutic strategies to block propagation of protein misfolding throughout the brain can be envisaged. It has been shown that TNTs might play a critical role in spreading of prion aggregates within the CNS and from the periphery. Therefore, the study of mechanism of TNT formation could provide new insights on the mechanism of disease propagation and novel therapeutic targets. The aim of my thesis was to study the role of TNT-mediate protein aggregates transfer between cells and to investigate the mechanism of TNT formation. In our lab, we already reported TNT mediate prion transfer between cells. In the first part of my PhD, I further confirmed that prion aggregates transfer between neuronal CAD cells through TNT inside endocytic vesicles (Zhu et al., 2015). Furthermore in collaboration with a colleague, we provided evidences that prion aggregates could transfer between primary astrocytes and neurons and the transfer was mediated by cell-to-cell contact (Victoria et al., 2016). I also collaborated to another study where we showed that α-synuclein aggregates (Parkinson’s disease) can transfer between cells inside lysosomes, and the intercellular transfer is mediated by TNTs (Abounit et al., 2016).In my second project, in order to investigate the mechanism of TNT formation, I performed a High-content screening of Rab GTPase. I found that Rab8 and Rab11 can promote TNT formation, that Rab8-VAMP3, Rab11-ERM and Rab8-Rab11 cascades are involved in TNT formation. My data suggests that both actin polymerization and membrane trafficking are involved in TNT formation. These results help to shed light on the mechanism of TNT formation, and provide molecular evidences that Rab GTPases regulate this process
Kelly, Douglas James. "An automated fluorescence lifetime imaging multiwell plate reader : application to high content imaging of protein interactions and label free readouts of cellular metabolism." Thesis, Imperial College London, 2014. http://hdl.handle.net/10044/1/29131.
Full textHuang, Jing. "DIFFERENTIAL ACTIVITY AND CONTENT OF HIGH-AFFINITY GLUTAMATE TRANSPORTERS, CONTENT OF THEIR REGULATORY PROTEINS, AND CAPACITY FOR GLUTAMINE AND GLUTATHIONE SYNTHESIS IN TISSUES OF FINISHED VERSUS GROWING STEERS." UKnowledge, 2017. https://uknowledge.uky.edu/animalsci_etds/81.
Full textPogna, Edgar Allan. "Phosphorylation and distribution of High-Mobility Group protein HMGN1 in the context of Immediate-Early (IE) gene induction." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:5b39d964-fd81-4534-bfba-76a32bb60f4e.
Full textJarvius, Malin. "Visualization of Protein Activity Status in situ Using Proximity Ligation Assays." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-131934.
Full textHeidlebaugh, Nancy Marie. "Analysis of nitrogen reallocation from senescing barley leaves characterization of the influence of a high-grain protein content locus on chromosome six, and molecular cloning and heterologous expression of a serine carboxypeptidase /." Thesis, Montana State University, 2008. http://etd.lib.montana.edu/etd/2008/heidlebaugh/HeidlebaughN0508.pdf.
Full textRåvik, Mattias. "Influence of Escherichia coli feedstock properties on the performance of primary protein purification." Licentiate thesis, KTH, Bioprocessteknik, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3941.
Full textQC 20101129
Råvik, Mattias. "Infuence of Escherichia coli feedstock properties on the performance of primary protein purification." Licentiate thesis, KTH, School of Biotechnology (BIO), 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3941.
Full textAbstract
The aim of the present study was to increase the understanding of how the cell surface properties affect the performance of unit operations used in primary protein purification. In particular, the purpose was to develop, set up and apply methods for studies of cell surface properties and cell interactions.
A method for microbial cell surface fingerprinting using surface plasmon resonance (SPR) is suggested. Four different Escherichia coli strains were used as model cells. Cell surface fingerprints were generated by registration of the interaction between the cells and four different surfaces, with different physical and chemical properties, when a cell suspension was flown over the surface. Significant differences in fingerprint pattern between some of the strains were observed. The physical properties of the cell surfaces were determined using microelectrophoresis, contact angle measurements and aqueous two-phase partitioning and were compared with the SPR fingerprints. The generated cell surface fingerprints and the physical property data were evaluated with multivariate data analysis that showed that the cells were separated into individual groups in a similar way using principal component analysis plots (PCA).
Studies of the behaviour of the model cells on stirred cell filtration and in an interaction test with different expanded bed adsorption (EBA) adsorbents were performed. It could be concluded that especially one of the strains behaved differently. Differences in the properties of the model cells were indicated by microelectrophoresis and aqueous two-phase partitioning which to some extent correlated with observed differences in behaviour during filtration and in an interaction test with EBA adsorbents.
The impact of high-pressure homogenisation of E. coli cell extract was examined, with a lab scale and a pilot scale technique. The DNA-fragmentation, visualised with agarose gel electrophoresis, and the resulting change in viscosity was analysed. A short homogenisation time resulted in increased viscosity of the process solution that correlated with increased concentration of released non-fragmented DNA. With longer homogenisation time the viscosity decreased with increasing degree of DNA-fragmentation.
The results show that strain dependant cell surface properties of E. coli may have an impact on several primary steps in downstream processing.
Benning, B. K. "Novel high water content hydrogels." Thesis, Aston University, 2000. http://publications.aston.ac.uk/9809/.
Full textAitken, Karen S. "Genetic analysis of grain protein content in wheat." Thesis, University of East Anglia, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357308.
Full textFeng, Tian. "High dynamic range visual content compression." Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/18315/.
Full textColin, Béatrice. "Développement d’un système rapporteur générique sensible, au double mode de lecture BRET/HCS, pour l’étude du suivi de gènes rapporteurs, de l’activité de protéase virale et des interactions protéine-protéine." Thesis, Lille 2, 2018. http://www.theses.fr/2018LIL2S050.
Full textSeveral reporter systems have been developed in order to support fundamental scientific projects, from gene promoter regulation study, signaling pathway activation, or protein/protein interaction monitoring.In order to enhance the sensitivity of a protease assay, we developed a new reporter system allowing signal amplification by optimizing a system based on the use of a hyperactive relay enzyme from Tobacco Etch Virus (TEV protease). The signal is detected by two methods compatible with high-throughput and leads to an ON/OFF signal. The Bioluminescence Resonance Energy Transfer (BRET) allows us to measure a signal at basal state, which will decrease upon cleavage of the probe by the protease. High Content Screening (HCS) Microscopy also allows the differentiation between positive and negative cells by a simple shift in the fluorescence acceptor location of the probe used in energy transfer, from the nucleus to the cytoplasm of the cells.The high sensitivity of our approach now leads us to validate its use in a more generic way. This is why the project aimed at the optimization of the BRET probe at the origin of the test, by performing a serie of amino acid deletions at the N-terminus of the energy donor and the C-terminus of the energy acceptor to find the best probe in order to obtain the best energy transfer. The HCS contrast between the two states was aslo increased by testing different nuclear localization sequences. With this new probe, we tried to adapt the system to several fields of application like i) a viral infection test using the HCV protease as a proof of concept but also ii) the expression of a reporter gene with the TGR5, a G-coupled protein receptor as model and iii) the detection of protein-protein interactions. To demonstrate this concept, we used the well know interaction between FRB and FKBP12 proteins, induced by rapamycine. Results from this project will lead to the development of a new reporter system, auto-amplified and usable in a generic way, which a very high sensitivity is necessary
DiÌaz, Maria Dolores FernaÌndez. "Effects of high pressure on protein protein interactions." Thesis, University of Reading, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270415.
Full textBartram, Christopher Gordon. "The endogenous protein content of ruminant proximal duodenal digesta." Thesis, University of Nottingham, 1987. http://eprints.nottingham.ac.uk/13952/.
Full textLewin, Erland. "Approaches to Optimizing High Content Confocal Microscopy." Licentiate thesis, KTH, Applied Physics, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-10691.
Full textThis thesis presents two methods for improving high contentconfocal microscopy.
The author's part in the first, "Toward a confocal subcellular atlasof the human proteome" was automating image capture of foursimultaneously tagged structures in cells in 96 well plates. In total,thousands of images of hundreds of proteins in cells. The authorwas also part of deciding which imaging methods should be used tomaximize image information content and quality, given the limitedtime available per well in order to scan large numbers of wells.
The second project, "Improved water permeability measurementsbased on fluorescence normalization" involves increasing the sensitivityof measurements of protein function by normalizing with anestimate of the amount of protein in the cell - the fluorescentsignal of a co-transfected protein. This could lead to achievingsufficient confidence in measurements with fewer experiments(thus increasing the information content in each experiment). Asurprisingly high level of noise in the relationship between thefluorescent signal and the protein function was measured.
Denna avhandling presenterar två projekt för att förbättrametoder för experiment med stora informationsmängderbaserade på konfokalmikroskopi.
Författarens del i det första projektet, "Toward a ConfocalSubcellular Atlas of the Human Proteome" (Mot en konfokal,subcellulär atlas av det mänskliga proteomet) var att automatiserabildinsamlingen av fyra samtidigt inmärkta strukturer i celler iplattor med 96 brunnar. Sammanlagt togs tusentals bilder avhundratals proteiner i celler. Författaren var även del i att fastställavilka bildinsamlingsmetoder som skulle användas för att maximeramängd och kvalitet på bild-informationen givet den begränsade tidper brunn som var tillgänglig för att kunna avbilda många brunnar.
Den andra studien, "Improved water permeability measurementsbased on fluorescence normalization" (Förbättrade vattenpermeabilitetsmätningargenom normalisering av fluorescens) syftade till att ökakänsligheten hos mätningar av proteiners funktion genom attnormalisera mätningarna med signalen från fluorescensen från ettkotransfekterat protein. Det skulle kunna leda till att nå tillräckligtillförlitlighet i mätresultaten med färre experiment (därmed ökainformationsinnehållet i varje experiment). En förvånansvärt högbrusnivå i förhållandet mellan fluorescenssignalen ochproteinfunktionen uppmättes
Wu, Mei. "Polymer microarrays for microbial high-content screening." Thesis, University of Edinburgh, 2012. http://hdl.handle.net/1842/7664.
Full textEkengren, Jens. "Estimating inclusion content in high performance steels." Licentiate thesis, Karlstad : Faculty of Technology and Science, Materials Engineering, Karlstads universitet, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-3520.
Full textOhlsson, Simon. "IGSCC in weld with high ferrite content." Thesis, KTH, Fysik, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-252691.
Full textJacques, Richard. "Statistical analysis of high content screening data." Thesis, University of Sheffield, 2009. http://etheses.whiterose.ac.uk/2220/.
Full textGros, Robert. "Regulation of G-protein-coupled receptor function, a role for increased G-protein-coupled receptor kinase-2 protein content." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0019/NQ58133.pdf.
Full textSadahira, Mitie Sônia 1964. "Effect of polysaccharide addition on the foaming properties of egg white protein in aqueous and high sugar contente systems = Efeito da adição de polissacarídeos nas propriedades espumantes de proteínas da clara de ovo em sistemas aquoso e com alto teor de açúcares." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256405.
Full textTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: Nos confeitos aerados (marshmallow e nougat), a espuma é produzida pela aeração de xaropes de açúcares, estabilizada por proteínas tais como proteínas da clara de ovo (PCO). A pectina, polissacarídeo aniônico, pode formar complexos eletrostáticos com proteína em pH abaixo do ponto isoelétrico da proteína. A hidroxipropilmetilcelulose (HPMC) é um polissacarídeo neutro com propriedades emulsificantes. O trabalho visou estudar as propriedades espumantes (capacidade de aeração e estabilidade da espuma) da PCO na presença destes polissacarídeos em solução aquosa e sistema modelo de açúcares. Na primeira etapa, foram avaliados os efeitos das interações PCO/polissacarídeo nas propriedades espumantes em solução aquosa. Os efeitos da concentração de biopolímeros (2,0-4,0% p/p), proporção PCO:pectina (15:1-55:1) e temperatura (70-80 °C) nas propriedades espumantes no pH 3,0 foram avaliados, utilizando delineamento composto central. Na proporção PCO:pectina 15:1, os complexos eram próximos da eletroneutralidade e com tamanho médio de 95,91+ ou - 8,19 µm, conduzindo para maior estabilidade da espuma quanto à desproporção. Na proporção 55:1, os complexos não eram eletricamente neutros e com tamanho médio de 45,92+ ou - 3,47 µm, resultando em espumas com menor drenagem de líquido e coalescência. Foram avaliados os efeitos de concentração de biopolímeros (2,0-5,0% p/p), proporção PCO:HPMC (2:1-18:1) e pH (3,0-6,0) a 75 °C utilizando delineamento composto central rotacional (DCCR) e do comportamento dos biopolímeros na solução aquosa em diferentes pH nas propriedades espumantes. No pH 3,0, os biopolímeros eram compatíveis, conduzindo a melhores propriedades espumantes enquanto nos pH 4,5 e 6,0, os biopolímeros eram incompatíveis, resultando em menor estabilidade com relação a desproporção. Na segunda etapa do trabalho, foram avaliados os efeitos das interações PCO/polissacarídeo em sistema modelo de açúcares com características de marshmallow (densidade<0,50 g/mL; atividade de água<0,75). A composição da solução de açúcares (42,5% sacarose, 42,5% xarope de glicose e 15% de açúcar invertido) foi definida utilizando delineamento experimental de mistura. Os efeitos da concentração de biopolímeros (1,40¿5,60% p/p) e proporção PCO:pectina (7:1¿63:1) nas respostas foram avaliadas utilizando um DCCR, no pH 3,0. As respostas foram viscosidade aparente da mistura açúcares/PCO/pectina antes do batimento e densidade, overrun, parâmetros reológicos da amostra aerada recém-processada e após 24 horas (módulo elástico G¿, módulo viscoso G" e 'delta'). Na proporção PCO:pectina 7:1, a mistura apresentou baixa capacidade de aeração e uma espuma com característica menos sólida e baixa estabilidade. Na proporção 49:1, a mistura apresentou maior capacidade de aeração e comportamento elástico da espuma. Os efeitos da concentração de biopolímeros (1,4-5,6% p/p) e proporção clara de ovo:HPMC (2:1-18:1) nas respostas das misturas açúcar/PCO/HPMC foram avaliados, utilizando um DCCR no pH 3,0 e as mesmas respostas avaliadas no estudo com misturas açúcar/PCO/pectina. Na concentração de biopolímeros 5,0% p/p e proporção PCO:HPMC 14:1 foram realizados experimentos em diferentes pH. No pH 3,0, foram obtidos maior capacidade de aeração e comportamento elástico. No pH 4,5, a espuma apresentou melhor estabilidade comparada a espuma no pH 3,0. No pH 6,0, a espuma apresentou propriedades espumantes ruins e comportamento viscoso. Portanto, o controle das interações proteína/polissacarídeo é um fator chave para o desenvolvimento de produtos aerados com maior estabilidade física
Abstract: In aerated confectionery (marshmallow and nougat), foam is produced by aeration of sugar syrups and stabilized by proteins such as egg white protein (EW). Pectin, an anionic polysaccharide, may form electrostatic complexes with protein at pH values bellow the isoeletric point (pI) of the protein. Hydroxypropylmethylcellulose (HPMC) is a neutral polysaccharide with emulsifying properties. The study aimed at studying the foaming properties (foaming capacity and foam stability) of EW in the presence of these polysaccharides in aqueous solution and high sugar system. Firstly, the effects of EW/polysaccharide interaction on the foaming properties in aqueous solution were evaluated. The effects of biopolymer concentration (2.0-4.0% w/w), EW:pectin ratio (15:1-55:1) and temperature (70-80 °C) were evaluated at pH 3.0, using a central composite design. At EW:pectin ratio 15:1, the complexes were close to electroneutrality and with an average size of 95.91+ or - 8.19 µm, leading to greater stability related to disproportionatin. At ratio 55:1, the complexes were not electrically neutral and with an average size of 45.92+ or - 3.47 µm, resulting in a low drainage of liquid and coalescence. The effects of biopolymer concentration (2.0-5.0% w/w), EW:HPMC ratio (2:1-18:1) and pH (3.0-6.0) at 75 °C were evaluated using central composite rotatable design (CCRD) and the behavior of biopolymer in aqueous solution on the foaming properties at different pH. At pH 3.0, EW and HPMC were compatible leading to better foaming properties whereas at pH 4.5 and 6.0, EW and HPMC were incompatible resulting in lower stability related to disproportionation. In the second part of the study, the effects of EW/polysaccharide interactions on a model system of sugar with characteristics of marshmallow (density<0.50 g/mL; water activity<0.75) were evaluated. For that, a sugar solution composition (42.5% of sucrose, 42.5% of glucose syrup and 15.0% of invert sugar) was defined by a mixture experimental design. The effects of biopolymer concentration (1.40-5.60% w/w) and EW:pectin ratio (7:1-63:1) on the reponses were evaluated using CCRD, at pH 3.0. The responses were apparent viscosity of sugar/EW/pectin mixture before whipping, overrun, foam density and, rheological parameters of fresh foam and foam aged for 24 h (elastic modulus G¿, viscous modulus G" and phase angle 'delta'). At EW:pectin ratio 7:1, the mixture showed low foaming capacity and a foam with less solid character and low stability. At ratio 49:1, the mixture presented greater foaming capacity and elastic behavior of foam. The effects of biopolymer concentration (1.4-5.6% w/w) and EW:HPMC ratio (2:1-18:1) on the responses of sugar/EW/HPMC mixtures were evaluated using CCRD at pH 3.0 and the same responses evaluated in the study of sugar/EW/pectin mixtures. At biopolymer concentration 5.0% w/w and EW:HPMC ratio 14:1, experiments were carried out at different pH. At pH 3.0, the higher foaming capacity and elastic behavior were obtained. At pH 4.5, foam showed better stability than foam at pH 3.0. At pH 6.0, foam presented the poorest foaming properties and viscous behavior. Thus, the control of protein/polysaccharide interactions is a key factor for the aerated products developing with higher stability
Doutorado
Consumo e Qualidade de Alimentos
Doutora em Alimentos e Nutrição
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Full textCataloged from PDF version of thesis.
Includes bibliographical references (p. 157-170).
High-throughput and high-content screening (HTS and HCS) of whole animals requires their immobilization for high-resolution imaging and manipulation. Here we present methods to enable HTS and HCS of the nematode Caenorhabditis elegans (C. elegans). First we present microfluidic technologies to rapidly isolate, immobilize, image and manipulate individual animals. These technologies include 1. a high-speed microfluidic sorter that can isolate and immobilize C. elegans in a well defined geometry for screening phenotypic features in physiologically active animals, 2. an integrated chip containing individually addressable screening-chamber devices for incubation and exposure of individual animals to biochemical compounds and high-resolution time-lapse imaging of multiple animals and 3. a design for delivery of compound libraries in standard multiwell plates to microfluidic devices and also for rapid dispensing of screened animals into multiwell plates. We then present an improved immobilization method that restrains animals with sufficient stability to perform femtosecond laser microsurgery and multiphoton imaging, without any apparent effects on animal health. We subsequently screen the contents of a small-molecule library for factors affecting neural regeneration following femtosecond laser microsurgery of C. elegans using these technologies. This screen identifies the kinase inhibitor staurosporine as a strong inhibitor of neural regeneration, and does so in a concentration and neuronal cell type-specific manner. Finally, we present a simple device for immobilizing C. elegans inside standard microtiter plates that is compatible with existing HTS systems. The device consists of an array of metal pins connected to individually-controlled thermoelectric coolers. 'We use this to perform femtosecond laser microsurgery on C. elegans in microtiter plates and to analyze the regeneration dynamics over time. This analysis shows that neurons tend regenerate in single short bursts that occur stochastically within the first two days post-surgery.
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Ph.D.
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Full text0048
新制・課程博士
博士(農学)
甲第19771号
農博第2167号
新制||農||1040(附属図書館)
学位論文||H28||N4987(農学部図書室)
32807
京都大学大学院農学研究科地域環境科学専攻
(主査)教授 飯田 訓久, 教授 近藤 直, 准教授 中村 公人
学位規則第4条第1項該当
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