Relevant bibliographies by topics / High and low protein wheat

Academic literature on the topic 'High and low protein wheat'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "High and low protein wheat"

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Pasikatan, M. C., and F. E. Dowell. "High-Speed NIR Segregation of High- and Low-Protein Single Wheat Seeds." Cereal Chemistry Journal 81, no. 1 (January 2004): 145–50. http://dx.doi.org/10.1094/cchem.2004.81.1.145.

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Mastilovic, Jasna, Daniela Horvat, Dragan Zivancev, Aleksandra Torbica, Zarko Kevresan, Nevena Djukic, Damir Magdic, and Gordana Simic. "Analysis of interrelations between wheat protein fractions composition and its technological quality with combined multivariate and univariate statistics." Chemical Industry 68, no. 3 (2014): 321–29. http://dx.doi.org/10.2298/hemind130313057m.

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Data on protein fractions proportion obtained with RP-HPLC and technological quality parameters for 29 wheat cultivars grown in Serbia and Croatia were used for studying of interrelations among wheat protein fractions with different solubility and molecular weight properties by multivariate (PCA) analysis. Obtained trends were used as the base for investigations related to differentiation of technological quality among wheat cultivars with different combination of protein fractions compositions using univariate statistics (ANOVA followed by Duncan?s test) in order to draw out information about interrelations between protein fractions proportion in wheat cultivars and their technological quality. Analysis based on first four PCA factors (89.04% of variability) pointed out at interdependences between: (1) high content of albumins and globulins, low gliadins content and gliadins/glutenins ratio, high ?-gliadins, LMW glutenins and low ?- gliadins share in total protein with low water absorption, high energy and high resistance to extensibility ratio, (2) high albumin and globulin content and high proportion of ?-gliadins in total protein and low extensigraph extensibility, (3) high share of high molecular weight glutenins (HMW-GS) in total proteins, high extensigraph resistance/extensibility ratio and (4) high ?-gliadins share in total protein and low extensigraph resistance/extensibility ratio.
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Cornish, G. B., D. J. Skylas, S. Siriamornpun, F. Békés, O. R. Larroque, C. W. Wrigley, and M. Wootton. "Grain proteins as markers of genetic traits in wheat." Australian Journal of Agricultural Research 52, no. 12 (2001): 1161. http://dx.doi.org/10.1071/ar01054.

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Different protein fractionation techniques were used to define differences between a set of 8 wheat lines used in genetic mapping studies in Australia. A proteomics approach was used to establish the feasibility of identifying new protein polymorphisms for mapping purposes. Detailed analysis confirmed differences in the glutenin subunits, gliadin proteins, and 10–20 other proteins, between the mapping population parents, Cranbrook, Halberd, CD87, and Katepwa. Differences were particularly evident in the low molecular weight classes of protein. Alternative technologies were used to determine the differences in various protein classes in order to screen doubled haploid lines derived from crosses between the wheat lines. Polyacrylamide gel electrophoresis analysis allowed the mapping of loci encoding high molecular weight (HMW) and low molecular weight (LMW) glutenin subunit proteins. Reversed phase high performance liquid chromatography also allowed several loci encoding LMW glutenin subunit proteins to be mapped, as well as a new protein on chromosome 6A. Capillary electrophoresis provided a high-resolution system that was used to map several gliadin-type proteins. The studies showed that proteins provide useful genetic markers and the data are discussed from the point of view of the advantages that protein-based markers offer in providing both genotypic and phenotypic data.
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Clarke, J. M., T. N. McCaig, R. M. DePauw, R. E. Knox, F. R. Clarke, M. R. Fernandez, and N. P. Ames. "Strongfield durum wheat." Canadian Journal of Plant Science 85, no. 3 (July 1, 2005): 651–54. http://dx.doi.org/10.4141/p04-119.

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Strongfield durum wheat (Triticum turgidum L. var durum) is adapted to the durum production area of the southern Canadian prairies. It combines high yield, high grain protein concentration, and low grain cadmium concentration. Strongfield has shorter, stronger straw than Kyle, and has similar maturity and disease resistance to other currently registered durum cultivars. Key words: Triticum turgidum L. var durum, durum wheat, cultivar description, yield, protein, disease resistance
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Clarke, J. M., R. E. Knox, R. M. DePauw, F. R. Clarke, T. N. McCaig, M. R. Fernandez, and A. K. Singh. "Eurostar durum wheat." Canadian Journal of Plant Science 89, no. 2 (March 1, 2009): 317–20. http://dx.doi.org/10.4141/cjps08129.

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Eurostar durum wheat [Triticum turgidum L. subsp. durum (Desf.) Husn.] is adapted to the durum production area of the Canadian prairies. It combines high grain yield, high grain protein concentration, very strong gluten, and low grain cadmium concentration. Eurostar has similar straw strength to Strongfield, and slightly later maturity and similar disease resistance to other currently registered durum cultivars. Key words: Triticum turgidum L. subsp. durum (Desf.) Husn., durum wheat, cultivar description, yield, protein, disease resistance
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Rahimi Eichi, Vahid, Mamoru Okamato, Stephan M. Haefele, Nathaniel Jewell, Chris Brien, Trevor Garnett, and Peter Langridge. "Understanding the Interactions between Biomass, Grain Production and Grain Protein Content in High and Low Protein Wheat Genotypes under Controlled Environments." Agronomy 9, no. 11 (November 1, 2019): 706. http://dx.doi.org/10.3390/agronomy9110706.

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Grain protein content (GPC) is a key quality attribute and an important marketing trait in wheat. In the current cropping systems worldwide, GPC is mostly determined by nitrogen (N) fertilizer application. The objectives of this study were to understand the differences in N response between high and low GPC wheat genotypes, and to assess the value of biomass growth analysis to assess the differences in N response. Six wheat genotypes from a range of high to low GPC were grown in low, medium and high N, under glasshouse conditions. This experiment was designed around non-destructive estimation of biomass using a high throughput image-based phenotyping system. Results showed that Spitfire and Mace had higher grain N% than Gazelle and QAL2000, and appeared to demand more N to grow their biomass. Moreover, at low N, Spitfire grew faster and achieved the maximum absolute growth rate earlier than high N-treated plants. High grain N% genotypes seem able to manage grain N reserves by compromising biomass production at low N. This study also indicated the importance of biomass growth analysis to show the differences in the N responsiveness of high and low GPC wheat.
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AGAPIE, Alina Laura, Cristian BOSTAN, and Nicolae Marinel HORABLAGA. "THE SULFUR IMPORTANCE FOR HIGH QUALITATIV PRODUCTIONS AT WINTER WHEAT." LIFE SCIENCE AND SUSTAINABLE DEVELOPMENT 1, no. 1 (April 24, 2020): 1–4. http://dx.doi.org/10.58509/lssd.v1i1.7.

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Wheat quality is a complex notion that involves more analysis parameters and the study of a large number of factors that act implicitly to determine it. Besides the genetic factor, mineral nutrition plays an important role in increasing the quality of the crops. Sulfur is an important element in plant nutrition, its absence causing similar damage to nitrogen deficiency. The experience was located at ARDS Lovrin, on a semicarbonatic chernozem, weakly-gleizated and weakly-alkalinised. The mobile P content in soil is 75.7 ppm, of mobile K is 205 ppm and the humus content is 3.47%. Four different agrofunds were studied, with the following graduations of experimental factors: N50, N50S20, N100 and N100S20. The aim of the paper is to highlight the importance of chemical sulfur fertilizers to increase the quality of wheat production. The analyzed parameters were: the percentage of protein, gluten, gliadin, glutenin and the accumulation of protein subunits with high and low molecular weight. Applying a dose of 20 kg/ha sulfur fertilizer active substance brings significant and very significant increases in the percentage of protein (2.8%) and gluten (6.5%), depending on the level of nitrogen fertilization used. The value of gluten proteins, gliadin and glutenin, expressed in g / 100 g flour, increases significantly when applying sulfur fertilizers. In conclusion, sulfur fertilizers, on the background of an adequate supply with nitrogen, help to achieve a balanced protein and gluten content and has a direct positive influence on gluten proteins content.
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Labuschagne, Maryke, Stefania Masci, Silvio Tundo, Vera Muccilli, Rosaria Saletti, and Angeline van Biljon. "Proteomic Analysis of Proteins Responsive to Drought and Low Temperature Stress in a Hard Red Spring Wheat Cultivar." Molecules 25, no. 6 (March 17, 2020): 1366. http://dx.doi.org/10.3390/molecules25061366.

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Drought stress is becoming more prevalent with global warming, and has been shown to have large effects on gluten proteins linked to wheat bread making quality. Likewise, low temperature stress can detrimentally affect proteins in wheat. This study was done to determine the differential abundance of high molecular weight (HMW) glutenin proteins in a drought and low temperature stressed high quality hard red spring wheat cultivar (PAN3478), against a control. The treatments were applied in the greenhouse at the soft dough stage. HMW glutenin proteins were extracted from the flour, and were separated by using two-dimensional gel electrophoresis. Protein spots that had p values lower than 0.05 and fold values equal to or greater than 1.2 were considered to be significantly differentially abundant. These proteins were further analyzed by using tandem mass spectrometry. There was a 1.3 to 1.8 fold change in 17 protein spots due to the cold treatment. The drought treatment caused a 1.3 to 3.8 fold change in 19 protein spots. These spots matched either HMW or low molecular weight (LMW) glutenin subunits. In the latter case, the C subunits of LMW glutenins were notably found to be up-regulated under both stress conditions. All the proteins that have been identified can directly influence dough characteristics. Data are available via ProteomeXchange with the identifier PXD017578.
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Fowler, D. B. "CDC Ptarmigan soft white winter wheat." Canadian Journal of Plant Science 90, no. 6 (December 1, 2010): 857–61. http://dx.doi.org/10.4141/cjps09181.

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CDC Ptarmigan is a soft white winter wheat (Triticum aestivum L.) cultivar that is eligible for grades of the Canada Western General Purpose (CWGP) wheat class. The CWGP class was introduced in 2007 to encourage the development and production of cultivars for the biofuel and livestock feed markets in western Canada. CDC Ptarmigan is an intermediate height, very high-yielding, stem and leaf rust susceptible, low protein concentration, soft kernel texture winter wheat cultivar that is adapted to the low rust hazard area of western Canada. A high yield potential of low protein concentration grain make CDC Ptarmigan a good fit for the CWGP class.
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Uri, Csilla, Árpád Tóth, Péter Sipos, Mária Borbélyné Varga, and Zoltán Győri. "The composition of gluten proteins and their effect on the rheological properties of gluten." Acta Agraria Debreceniensis, no. 23 (May 23, 2006): 124–29. http://dx.doi.org/10.34101/actaagrar/23/3215.

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Wheat is the major cereal component of bread in the world and is grown worldwide. Of the cereals only the bread wheats – and less the triticale – includes storage proteins that play an important role in the performance of gluten. Proteins of gluten complex may be present in two classes:− low molecular weight (gliadin-) components, and− high molecular weight (glutenin-) components.Gliadins shown appreciable heterogenity and can be separated into 40-50 components with gel electrophoresis. The composition of gliadins is employable for the identification the wheat varieties and to investigate the varieties. In the decreasing electrophoretic mobility sequence may be distinguish α-, β-, γ- and ω-gliadins. A glutenin subunits may be include in two classes:− high molecular weight glutenin subunits (HMW-GS),− low molecular weight glutenin subunits (LMW-GS).Wheat varieties can be identified by glutenin and their quality selection is also possible. The gliadin’s polypeptides encoding genes are located on the short arm of chromosomes 1A, 1B and 1D, 6A, 6B and 6D. Genetic coding for HMW subunits is located on the long arms of chromosomes 1A, 1B and 1D, the LMW-GS are also located on chromosomes 1A, 1B and 1D (Glu-3 loci) near the gliadin-coding loci.Storage proteins affect the rheological properties of gluten by two factors:1. The quality and quantity of the protein components of the gluten complex,2. The interactions between the protein fractions.
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Dissertations / Theses on the topic "High and low protein wheat"

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Toma, Kumika. "Effects of High-Carbohydrate and Low-Fat Versus High-Protein and Low-Carbohydrate Diets on High-Intensity Aerobic Exercise." View abstract, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3372362.

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Ottman, Michael J., Stephen H. Husman, and Pat A. Clay. "Use of Tissue Testing to Prevent Low Grain Protein Content in Durum, 2003." College of Agriculture, University of Arizona (Tucson, AZ), 2007. http://hdl.handle.net/10150/203660.

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Low grain protein content in durum can be prevented by applying nitrogen fertilizer after heading. Tentative guidelines were established from previous research for nitrogen fertilizer applications after heading based on the lower stem nitrate content near heading. Ten commercial durum fields were selected for testing the use of these guidelines to ensure grain protein contents greater than 13%. Only one field had grain protein content less than 13% (12.83%), and this field had herbicide damage and had to be over-irrigated due to surface unevenness. The average protein content was 13.62% but the amount of nitrogen fertilizer actually applied by the growers after heading averaged 74.5 lbs N/acre, whereas the amount recommended by the tentative guidelines averaged 53.1 lbs N/acre. If the tentative guidelines had been followed, we estimate that the average grain protein content would have been about 13.04%. Our tentative nitrogen fertilizer recommendations based on stem samples near heading appear accurate, but another year of testing would add more certainty.
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Ottman, Michael J., Stephen H. Husman, and Pat A. Clay. "Use of Tissue Testing to Prevent Low Grain Protein Content in Durum, 2004." College of Agriculture, University of Arizona (Tucson, AZ), 2007. http://hdl.handle.net/10150/203661.

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Low grain protein content in durum can be prevented by applying nitrogen fertilizer after heading. Tentative guidelines were established from previous research for nitrogen fertilizer applications after heading based on the lower stem nitrate content near heading. Ten commercial durum fields were selected for testing the use of these guidelines to ensure grain protein contents greater than 13%. The average protein content was 14.00%, the amount of nitrogen fertilizer actually applied by the growers after heading averaged 44.5 lbs N/acre, whereas the amount recommended by the tentative guidelines averaged 41.5 lbs N/acre. If the tentative guidelines had been followed, we estimate that the average grain protein content would have been about 13.92%, and two fields would have been slightly below 13% protein (about 12.8% protein). Our tentative nitrogen fertilizer recommendations based on stem samples near heading appear accurate.
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Ottman, Michael J., and Stephen H. Husman. "Use of Tissue Testing to Prevent Low Grain Protein Content in Durum, 2005." College of Agriculture, University of Arizona (Tucson, AZ), 2007. http://hdl.handle.net/10150/203662.

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Low grain protein content in durum can be prevented by applying nitrogen fertilizer after heading. Tentative guidelines were established from previous research for nitrogen fertilizer applications after heading based on the lower stem nitrate content near heading. Three durum fields in Pinal County were selected for testing the use of these guidelines for ensuring grain protein contents greater than 13%. These fields were split into plots that either received late N fertilization after heading or not. The stem nitrate content at heading for two of the fields averaged 6337 ppm, indicating no need for late N fertilizer application to achieve grain protein content above 13%, and the grain protein content for these fields averaged 15.1% with or without late N fertilizer. The stem nitrate content at heading was 894 ppm for the third field, the stem nitrate guidelines called for a late N application of about 63 lbs N/a, and a late N application of 46 lbs N/a increased grain yield protein from 11.54 to 13.34%. Our tentative nitrogen fertilizer recommendations based on stem samples near heading appear accurate.
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Monaghan, James Malcolm. "Physiological and genetical analysis of high grain protein concentration, high yield trait in winter wheat (Triticum aestivum L.)." Thesis, Open University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361593.

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Riggs, Amy Jo Gropper Sareen Annora Stepnick. "Changes in energy expenditure associated with injestion of high protein, high fat versus high protein, low fat meals among underweight, normal weight, and overweight females." Auburn, Ala., 2006. http://repo.lib.auburn.edu/2006%20Spring/doctoral/RIGGS_AMY_28.pdf.

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Werner, Tim J. "The effect of high-carbohydrate, low-fat & low-carbohydrate, high protein diets on physiologic and performance variables on row ergometry training." Ohio : Ohio University, 2006. http://www.ohiolink.edu/etd/view.cgi?ohiou1140557597.

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Werner, Tim. "The Effect of High-Carbohydrate, Low-Fat & Low-Carbohydrate, High Protein Diets on Physiologic and Performance Variables on Row Ergometry Training." Ohio University / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1140557597.

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Dubey, Amrita. "Use of Extrusion Technology and Fat Replacers to Produce High Protein, Low Fat Cheese." DigitalCommons@USU, 2011. http://digitalcommons.usu.edu/etd/961.

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This study investigated the use of extrusion technology and fat replacers to produce high protein, low fat Cheddar cheese. In chapter 3, four different fat replacers were tested at the highest concentration level of each, as recommended by the manufacturers for low fat cheese, to investigate the change in cheese texture and optimize extruder conditions. In addition, the press time/pressure combinations of the extruded cheeses were optimized. The fat replacers and extruder conditions that were effective in improving the texture of low fat cheese were then used in chapter 4. In chapter 4, three fat replacers were used at three different concentrations (lowest, middle and highest) as recommended by the manufacturers for replacing fat in cheese. The fat replacers were microcrystalline cellulose (MCC 1) (0.125%, 1.06% and 2%), whey protein concentrate (WPC 2) (0.50%, 0.75% and 1%) and whey protein concentrate (WPC 1) (0.40%, 2.20% and 4%). These fat replacers were effective in improving the texture of low fat cheese as determined from the results of chapter 3. The extruded cheese samples with and without fat replacers were analyzed for texture at three different time periods (1 day, 1 week, and 1 month). None of the fat replacers used were effective in improving the texture of low fat cheese significantly. Since none of the treatments statistically improved the texture of low fat cheese, in the next part of the study, extrusion alone and WPC 1 at the middle concentration were then used to produce low fat cheese with high protein content by blending low moisture aged Cheddar cheese and nonfat cheese. Extrusion of cheese blends with or without fat replacer yielded cheese with high protein level. It was concluded from the study that the fat replacers we used were not effective in improving the texture but extrusion of aged Cheddar cheese with nonfat cheese can yield high protein cheese.
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Spain, James Nobles. "Effect of protein source on milk composition of cows fed low fiber, high grain diets." Thesis, This resource online, 1987. http://scholar.lib.vt.edu/theses/available/etd-04122010-083607/.

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Books on the topic "High and low protein wheat"

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Eckhardt, Linda West. The high protein cookbook. New York: Clarkson Potter, 2000.

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V, Clark Charles. The new high protein diet. London: Vermilion, 2002.

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OMG, that's paleo? Royal Oak, Michigan: Scribe Publishing Company, 2013.

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Dan, Eades Mary, and Eades Michael R, eds. The protein power lifeplan gram counter. New York, NY: Warner Books, 2000.

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Media, Springer Science+Business, ed. Protein downstream processing: Design, development and application of high and low-resolution methods. New York: Humana Press, 2014.

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Carboni, Camilla. Paleo cleanse: 30 days of ancestral eating to detox, drop pounds, supercharge your health and transition into a primal lifestyle. Berkeley, CA: Ulysses Press, 2014.

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Baker, Yvonne G. Guilt-free cooking: An introduction to naturally good cooking for those who love to eat & want to do it right. Denver, CO: Accent Books, 1985.

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Susan, Grodnick, ed. The Four Seasons Spa Cuisine. New York: Simon and Schuster, 1986.

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Conrad, Anna. Paleo Dessert Bible: More Than 100 Delicious Recipes for Grain-Free, Dairy-Free Desserts. Skyhorse Publishing Company, Incorporated, 2016.

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Photography, J. Stanfield, ed. The paleo dessert bible: More than 100 delicious recipes for grain-free, dairy-free desserts. Skyhorse, 2016.

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Book chapters on the topic "High and low protein wheat"

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Jankulovska, Mirjana, Sonja Ivanovska, Ljupcho Jankuloski, Mile Markoski, Biljana Kuzmanovska, and Dane Boshev. "Evaluation of advanced wheat mutant lines for food and feed quality." In Mutation breeding, genetic diversity and crop adaptation to climate change, 209–19. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789249095.0021.

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Abstract The main goals of this study were to evaluate the agronomic performance of wheat mutant lines; to detect the effect of genotype, location and different fertilizer levels on analysed traits; to assess seed and feed quality; and to select best performing mutant lines for dual-purpose growing. Ten wheat mutant lines were sown on two locations in Macedonia, for evaluation of their agronomic performance. At both locations, grain yield, straw mass, harvest index, nitrogen use efficiency, nitrogen and protein content in seed and straw, neutral detergent fibre and acid detergent fibre in the straw were determined. In order to classify the genotypes based on all analysed traits, two-way cluster analysis was applied. According to their overall performance, at both locations and with the three different fertilization treatments, the mutant lines were classified in two main groups. The first cluster consisted of mutants 5/1-8, 2/2-21, 4/2-56 and 2/1-51, characterized by very high values for seed yield, straw yield and harvest index, and high to moderate values for all other traits. Only 4/2-56 had very low values for N and protein content in the seed. One mutant line, 6/2-2, did not belong to any of the groups and differed from all other genotypes based on its very low seed and straw yield and very high values for nitrogen and protein content in the straw and neutral detergent fibre. All other mutants belonged to the second group, with low to moderate yield and moderate to high values for the other traits. Mutant lines with the highest seed and straw yield, as well as the best quality of seed and straw under different management systems, were identified and after additional evaluation will be submitted for official variety registration.
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Feldman, M., L. Avivi, A. A. Levy, M. Zaccai, Y. Avivi, and E. Millet. "High Protein Wheat." In Biotechnology in Agriculture and Forestry, 593–614. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-662-10933-5_33.

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Rincón, Magaly, and Robert A. Gonzales. "Induction of protein synthesis by aluminium in wheat (Triticum Aestivum L.) Root Tips." In Plant-Soil Interactions at Low pH, 851–58. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3438-5_95.

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Zolla, L., C. G. Huber, A. M. Timperio, M. Bianchetti, and D. Corradini. "Separation and Characterisation of Wheat Protein PSII by High Resolution Chromatography Techniques." In Photosynthesis: Mechanisms and Effects, 4377–80. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-3953-3_1013.

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Picton, Steve J., Keith D. Richards, and Richard C. Gardner. "Protein profiles in root-tips of two wheat (Triticum aestivum L.) cultivars with differential tolerance to aluminium." In Plant-Soil Interactions at Low pH, 1063–70. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3438-5_119.

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Burckhardt, Peter. "The Negative Effect of a High-Protein–Low-Calcium Diet." In Nutritional Influences on Bone Health, 125–31. London: Springer London, 2013. http://dx.doi.org/10.1007/978-1-4471-2769-7_12.

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Langridge, Peter. "Micronutrient Toxicity and Deficiency." In Wheat Improvement, 433–49. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-90673-3_24.

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AbstractMicronutrients are essential for plant growth although required in only very small amounts. There are eight micronutrients needed for healthy growth of wheat: chlorine, iron, boron, manganese, zinc, copper, nickel and molybdenum. Several factors will influence the availability of micronutrients, including levels in the soil, and mobility or availability. Zinc deficiency is the most significant problem globally followed by boron, molybdenum, copper, manganese and iron. Deficiency is usually addressed through application of nutrients to seeds, or through foliar spays when symptoms develop. There is considerable genetic variation in the efficiency of micronutrient uptake in wheat, but this is not a major selection target for breeding programs given the agronomic solutions. However, for some micronutrients, the concentrations in the soil can be very high and result in toxicity. Of the micronutrients, the narrowest range between deficiency and toxicity is for boron and toxicity is a significant problem in some regions. Although not a micronutrient, aluminium toxicity is also a major factor limiting yield in many areas, usually associated with a low soil pH. Agronomic solutions for boron and aluminium toxicity are difficult and expensive. Consequently, genetic approaches have dominated the strategies for addressing toxicity and good sources of tolerance are available.
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Kovalchuk, Nataliya. "High-Throughput Analysis Pipeline for Achieving Simple Low-Copy Wheat and Barley Transgenics." In Methods in Molecular Biology, 239–52. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0446-4_18.

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Rodrigues, F. A., I. Ortiz-Monasterio, P. J. Zarco-Tejada, U. Schulthess, and B. Gérard. "High resolution remote and proximal sensing to assess low and high yield areas in a wheat field." In Precision agriculture '15, 191–98. The Netherlands: Wageningen Academic Publishers, 2015. http://dx.doi.org/10.3920/978-90-8686-814-8_23.

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Grosfils, Patrick. "Effects of Protein Size on the High-Concentration/Low-Concentration Phase Transition." In Advances in Chemical Physics, 173–91. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. http://dx.doi.org/10.1002/9781118309513.ch7.

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Conference papers on the topic "High and low protein wheat"

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L'Hocine, Lamia, Allaoua Achouri, Emily Mason, and Mélanie Pitre. "Allergenicity risk assessment of glabrous canaryseed as novel food protein source." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/hyzq5376.

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Glabrous canaryseed, a novel cereal grain, is emerging as a valuable source of plant proteins due to its high content in protein (22%). This true cereal was approved for human consumption in Canada and the United States, and as part of the regulatory safety assessment, its allergenic potential was evaluated. Canaryseed was found to be gluten-free and thus, suitable for individuals with celiac disease, however, possible allergic cross-reactivity between canaryseed and wheat was also revealed. Based on these findings, a cautionary labelling alluding to the potential of allergic reaction is requested on canaryseed food products, and further research to clarify the relationship between canary seed proteins and known wheat allergens was recommended. Therefore, the purpose of this study was to further assess the immunological cross-reactivity risks of canary seed to phylogenetically related grains, including wheat and oat, using wheat-allergic sera IgE based 1D and 2D- immunoblots and ELISA, followed by proteomic/bioinformatics identification of IgE-binding proteins. The results demonstrated extensive serological cross-reactivity between wheat, oat and canaryseed proteins, where the less abundant protein fractions showed the strongest IgE-binding. The in-gel tryptic digestion and LC-MS/MS identification of the IgE-binding canaryseed proteins showed high homology to proteins from wheat, barley, oat and Brachypodium distachyon (also known as stiff brome), which all belong to the Pooideae botanical subfamily. A majority of the IgE-binding proteins were mostly minor metabolic enzymes or uncharacterized proteins. Low sequence homology was observed for the 11-12S globulin storage proteins. Positive serological testing cannot ascertain allergic reaction to canaryseed, it does not rule out, however, the risks for wheat, oat or barley sensitized atopic population. Clinical oral food challenge remains the ultimate tool to conclude on the allergenicity of canaryseed. Until then, these data serve the reinforcement of the regulatory requirement to use allergen precautionary labeling for products containing canaryseed proteins.
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"Identification of wheat varieties with high grain protein and gluten content." In Current Challenges in Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk State University, 2019. http://dx.doi.org/10.18699/icg-plantgen2019-03.

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Mody, Jaisen, Roman Saveliev, Ezra Bar-Ziv, and Miron Perelman. "Selection of Biomass Feedstock for Production of Biocoal for Coal-Fired Boilers." In ASME 2014 Power Conference. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/power2014-32031.

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PGE in collaboration with EBC and MTU is carrying out a testing program to fire up to 100% of biocoal (torrefied biomass) in its 600 MW Boardman boiler. An important aspect of this program is the selection of suitable biomass feedstock from which biocoal will be produced, emphasizing potential problems of fouling and slagging in the boiler. We thoroughly tested seven different types of feedstock: Arundo Donax (AD), wheat waste, corn waste, woody hybrid poplar, and bark from hybrid poplar, woody pine, and bark from pine. It was found that all these material comprised significant amounts of soil (varying from 5–25% in weight) with low fusion temperatures and therefore must be avoided from flowing into the boiler. We developed a separation technology of the soil from the biomass and were able to obtain biomass feedstock only with the plant minerals. All separated biomass feedstock, from soil, showed mineral content that is respective to soil they grew at. Samples were characterized for ultimate and proximate analysis, ash content and analysis and fusion temperatures. AD, wheat, and corn showed high content of potassium and low flow temperatures and therefore may not be used at 100% firing test unless some of the mineral contents are removed to protect the boiler from corrosion and slagging. Woody and bark hybrid poplar were found to have high fusion temperatures; woody and bark pine showed flow temperatures around 2500°F. All four feedstock types can be used for 100% firing test, however, the ones which is mostly recommended are woody and bark hybrid poplar.
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"Sources of high protein and gluten content in grain in some wheat species." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-015.

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Mannuccl, P. M., and A. Tripodl. "DIAGNOSTIC SCREENING OF CONGENITAL THROMBOTIC SYNDROMES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643717.

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The prevalence of inherited thrombotic syndromes in the general population (1 in 2,500/5,000) appears to be higher than that of inherited bleeding disorders. We have reviewed the problems of their diagnosis and propose a simple screening procedure. The most important candidates far. screening are patients with unexplained venous thromboembolism at ages ofless than 40 years, particularly when thrombotic episodes are recurrent.Screening must start from collectionof the clinical and family history of the propositus and from the exclusion of common acquired forms of thrombophilia. A negative family historydoes not exclude inherited thrombophilia, because the defects have oftena low penetrance and fresh mutationsmay have occurred in the propositi. The test chosen for laboratoryscreening of inherited thrombotic syndromes must be limited in number, easy todo and, more importantly, their results should be clinically relevent Which defects should be screened and what type of methodology should be used? The table is intended to answerthese questions by proposing a two-step screening procedure.The tests included in the .first step of the screening are aimed at evaluating Laboratory screening of inherited thrombotic syndromes the most frequent and well established causes of inherited thrombophilia, —-antithrombin III, protein C. protein S.plasminogen and fibrinogen.FIRST STEP Antithrombin III (heparin cofactorI chromogenic assay)Protein C (Francis' clotting assay)Protein S(electroimmunoassay of total proteinSantigen)Plasminogen (chromogenic assay)Fibrinogen (clotting assay)SECONSTEP(Tran's functional assay) Plasminogen activator (fibrin plate assay before and after venous stasisor DDAVP)Plasminogen activator inhibitor(chromogenic assay)The tests offirst choice that we propose (see table) are in general functional assaysdetecting both type I and type IIdeficiencies and are simple enough tobecarried out even in non specialized laboratories.For protein S, however,this goal has not been achieved yet and only type I protein S deficiencycan be currently identified with immunoassays measuring total protein S antigen. Since a number of laboratories may still not have the facilities to perform protein C functional assays, they are advised to set up at least an immunoassay, since type I deficiencies are much more frequent than type II deficiencies. The tests included in the second step of the screening are aimed at detectingthe less common or less well established causes of thrombophilia, and should be carried out when the clinical history suggests the existence of inherited thrombophilia and yet the first step has failed to reveal any laboratory abnormality. Defective plasminogen activation can be evaluated by measuring plasminogen activator activity with the simple fibrin plate assay carried out before and after stimuli such as venous occlusion and/or DDAVP infusion. The parallel measurement of plasminogen activator inhibitor allows to distinguish cases of detective plasminogen activation due to high inhibitor levels. The measurement of heparin cofactor II should also be included in this battery of second-step screening tests.Using this screening procedure in95 propositi with juvenile venous thromboembolism, we have identified 7 kindreds with antithrombin III deficiency (5 type I and 2 type II) (7.5%),7 kindreds with protein C deficiency (1 type II) (7.5%), 5 kindredswith protein S deficiency (5%), 1 withhypoplasminogenemia (1%) and 1 with dysfibrinogenemia Milano II (1). Theremaining undiagnosed cases might bedue to as yet unidentified deficiencies or abnormalities of other antithrombotic mechanisms such as,for instance, endothelial thrombomodulin or the fibrinolysis enhancing property of the protein C-protein S system.
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Schwarz, H. P., and W. Muntean. "LOW TOTAL PROTEIN S ANTIGEN BUT HIGH PROTEIN S ACTIVITY DUE TO DECREASED C4b-BINDING PROTEIN (C4b-BP) LEVELS IN NEWBORNS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643610.

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Vitamin K-dependent coagulation proteins are known to be decreased in the neonatal period. So far no data have been published on protein S (PS), the vitamin K-dependent cofactor for the antithrombotic enzyme, activated protein C (APC) in this period. We determined, therefore, PS antigen, PS activity and C4b-BP,a regulatory protein of the classical complement pathway to which PS is complexed, in 36 neonates. Total PS antigen in newborns was below the range associated with thromboembolism in patients congenitally deficient in this protein (22±9.6%, mean±SD). None of these infants had clinical or laboratory evidence of thromboembolism or DIC. In contrast to the PS antigen level PS activity measured by the ability of APC to prolong the clotting time of a modified APTT assay using PS-immunodep1eted plasma was significantly higher (77.6±14%, mean±SD, p< 0,001), suggesting a shift in PS to the free form. In fact two dimensional immunoe1ectrophoresis studies revealed the absence of protein S-C4b-BP complexes and only one precipitation indicating free PS was seen in 15 out of the 36 infants. In these 15 neonates C4b-BP was below the limit of detection by sensitive quantitative immunob1otting techniques using monoclonal or polyclonal antibodies. In the remaining 21 infants PS-C4b-BP complexes were detected, but in contrast to adult normal plasma approximately 80% of PS was found in the free form. Mixing experiments with normal human plasma and newborn’s plasma indicate that PS in neonate deficient of C4b-BP can bind normally to C4bp. Absence of C4b-BP did not correlate to gestational age. If an equilibrium distribution of PS between bound and free form regulates the cofactor activity of PS for the anticoagulant and profibrino 1ytic properties of APC in normal adults, our study demonstrates that the absence of PS-C4b-BP complexes in newborns and the presence of free PS only may contribute to the increased bleeding risk of premature infants.
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Olson, Brian, and Amarda Shehu. "Jumping low, jumping high: Controlling hopping in the protein energy surface." In 2012 IEEE International Conference on Bioinformatics and Biomedicine Workshops (BIBMW). IEEE, 2012. http://dx.doi.org/10.1109/bibmw.2012.6470276.

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Castaño-Martinez, T., and T. Laeger. "Low-protein/high-fat diet induces partial remission of type 2 diabetes." In Abstracts des Adipositas-Kongresses 2020 zur 36. Jahrestagung der Deutschen Adipositas Gesellschaft e.V. (DAG). © Georg Thieme Verlag KG, 2020. http://dx.doi.org/10.1055/s-0040-1714490.

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Hurkman, William J., and Delilah F. Wood. "Starch granule formation and protein deposition in wheat ( Triticum aestivum L.) starchy endosperm cells is altered by high temperature during grain fill." In Scanning Microscopy 2010, edited by Michael T. Postek, Dale E. Newbury, S. Frank Platek, and David C. Joy. SPIE, 2010. http://dx.doi.org/10.1117/12.853183.

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Andreini, E. M., S. M. Augenstein, C. S. Fales, R. D. Sainz, and J. W. Oltjen. "Effects of feeding level on efficiency of high and low residual feed intake beef steers." In 6th EAAP International Symposium on Energy and Protein Metabolism and Nutrition. The Netherlands: Wageningen Academic Publishers, 2019. http://dx.doi.org/10.3920/978-90-8686-891-9_10.

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Reports on the topic "High and low protein wheat"

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Dubcovsky, Jorge, Tzion Fahima, Ann Blechl, and Phillip San Miguel. Validation of a candidate gene for increased grain protein content in wheat. United States Department of Agriculture, January 2007. http://dx.doi.org/10.32747/2007.7695857.bard.

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High Grain Protein Content (GPC) of wheat is important for improved nutritional value and industrial quality. However, selection for this trait is limited by our poor understanding of the genes involved in the accumulation of protein in the grain. A gene with a large effect on GPC was detected on the short arm of chromosome 6B in a Triticum turgidum ssp. dicoccoides accession from Israel (DIC, hereafter). During the previous BARD project we constructed a half-million clones Bacterial Artificial Chromosome (BAC) library of tetraploid wheat including the high GPC allele from DIC and mapped the GPC-B1 locus within a 0.3-cM interval. Our long-term goal is to provide a better understanding of the genes controlling grain protein content in wheat. The specific objectives of the current project were to: (1) complete the positional cloning of the GPC-B1 candidate gene; (2) characterize the allelic variation and (3) expression profile of the candidate gene; and (4) validate this gene by using a transgenic RNAi approach to reduce the GPC transcript levels. To achieve these goals we constructed a 245-kb physical map of the GPC-B1 region. Tetraploid and hexaploid wheat lines carrying this 245-kb DIC segment showed delayed senescence and increased GPC and grain micronutrients. The complete sequencing of this region revealed five genes. A high-resolution genetic map, based on approximately 9,000 gametes and new molecular markers enabled us to delimit the GPC-B1 locus to a 7.4-kb region. Complete linkage of the 7.4-kb region with earlier senescence and increase in GPC, Zn, and Fe concentrations in the grain suggested that GPC-B1 is a single gene with multiple pleiotropic effects. The annotation of this 7.4-kb region identified a single gene, encoding a NAC transcription factor, designated as NAM-B1. Allelic variation studies demonstrated that the ancestral wild wheat allele encodes a functional NAC transcription factor whereas modern wheat varieties carry a non-functional NAM-B1 allele. Quantitative PCR showed that transcript levels for the multiple NAMhomologues were low in flag leaves prior to anthesis, after which their levels increased significantly towards grain maturity. Reduction in RNA levels of the multiple NAMhomologues by RNA interference delayed senescence by over three weeks and reduced wheat grain protein, Zn, and Fe content by over 30%. In the transgenic RNAi plants, residual N, Zn and Fe in the dry leaves was significantly higher than in the control plants, confirming a more efficient nutrient remobilization in the presence of higher levels of GPC. The multiple pleiotropic effects of NAM genes suggest a central role for these genes as transcriptional regulators of multiple processes during leaf senescence, including nutrient remobilization to the developing grain. The cloning of GPC-B1 provides a direct link between the regulation of senescence and nutrient remobilization and an entry point to characterize the genes regulating these two processes. This may contribute to their more efficient manipulation in crops and translate into food with enhanced nutritional value. The characterization of the GPC-B1 gene will have a significant impact on wheat production in many regions of the world and will open the door for the identification of additional genes involved in the accumulation of protein in the grain.
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Blum, Abraham, Henry T. Nguyen, and N. Y. Klueva. The Genetics of Heat Shock Proteins in Wheat in Relation to Heat Tolerance and Yield. United States Department of Agriculture, August 1993. http://dx.doi.org/10.32747/1993.7568105.bard.

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Fifty six diverse spring wheat cultivars were evaluated for genetic variation and heritability for thermotolerance in terms of cell-membrane stability (CMS) and triphenyl tetrazolium chloride (TTC) reduction. The most divergent cultivars for thermotolerance (Danbata-tolerant and Nacozari-susceptible) were crossed to develop an F8 random onbred line (RIL) population. This population was evaluated for co-segragation in CMS, yield under heat stress and HSP accumulation. Further studies of thermotolerance in relations to HSP and the expression of heterosis for growth under heat stress were performed with F1 hybrids of wheat and their parental cultivars. CMS in 95 RILs ranged from 76.5% to 22.4% with 71.5% and 31.3% in Danbata and Nacozari, respectively. The population segregated with a normal distribution across the full range of the parental values. Yield and biomass under non-stress conditions during the normal winter season at Bet Dagan dit not differ between the two parental cultivar, but the range of segregation for these traits in 138 RILs was very high and distinctly transgressive with a CV of 35.3% and 42.4% among lines for biomass and yield, respectively. Mean biomass and yield of the population was reduced about twofold when grown under the hot summer conditions (irrigated) at Bet Dagan. Segregation for biomass and yield was decreased relative to the normal winter conditions with CV of 20.2% and 23.3% among lines for biomass and yield, respectively. However, contrary to non-stress conditions, the parental cultivars differed about twofold in biomass and yield under heat stress and the population segregated with normal distribution across the full range of this difference. CMS was highly and positively correlated across 79 RILs with biomass (r=0.62**) and yield (r=0.58**) under heat stress. No such correlation was obtained under the normal winter conditions. All RILs expressed a set of HSPs under heat shock (37oC for 2 h). No variation was detected among RILs in high molecular weight HSP isoforms and they were similar to the patterns of the parental cultivars. There was a surprisingly low variability in low molecular weight HSP isoforms. Only one low molecular weight and Nacozari-specific HSP isoform (belonging to HSP 16.9 family) appeared to segregate among all RILs, but it was not quantitatively correlated with any parameter of plant production under heat stress or with CMS in this population. It is concluded that this Danbata/Nacozari F8 RIL population co-segregated well for thermotolerance and yield under heat stress and that CMS could predict the relative productivity of lines under chronic heat stress. Regretfully this population did not express meaningful variability for HSP accumulation under heat shock and therefore no role could be seen for HSP in the heat tolerance of this population. In the study of seven F1 hybrids and their parent cultivars it was found that heterosis (superiority of the F1 over the best parent) for CMs was generally lower than that for growth under heat stress. Hybrids varied in the rate of heterosis for growth at normal (15o/25o) and at high (25o/35o) temperatures. In certain hybrids heterosis for growth significantly increased at high temperature as compared with normal temperature, suggesting temperature-dependent heterosis. Generally, under normal temperature, only limited qualitative variation was detected in the patterns of protein synthesis in four wheat hybrids and their parents. However, a singular protein (C47/5.88) was specifically expressed only in the most heterotic hybrid at normal temperature but not in its parent cultivars. Parental cultivars were significantly different in the sets of synthesized HSP at 37o. No qualitative changes in the patterns of protein expression under heat stress were correlated with heterosis. However, a quantitative increase in certain low molecular weight HSP (mainly H14/5.5 and H14.5.6, belonging to the HSP16.9 family) was positively associated with greater heterosis for growth at high temperature. None of these proteins were correlated with CMS across hybrids. These results support the concept of temperature-dependent heterosis for growth and a possible role for HSP 16.9 family in this respect. Finally, when all experiments are viewed together, it is encouraging to find that genetic variation in wheat yield under chronic heat stress is associated with and well predicted by CMS as an assay of thermotolerance. On the other hand the results for HSP are elusive. While very low genetic variation was expressed for HSP in the RIL population, a unique low molecular weight HSP (of the HSP 16.9 family) could be associated with temperature dependant heterosis for growth.
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Brice, Jeremy. Investment, power and protein in sub-Saharan Africa. Edited by Tara Garnett. TABLE, October 2022. http://dx.doi.org/10.56661/d8817170.

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The place of protein in sub-Saharan Africa’s food system is changing rapidly, raising complex international development, global health and environmental sustainability issues. Despite substantial growth in the region’s livestock agriculture sector, protein consumption per capita remains low, and high levels of undernourishment persist. Meanwhile sub-Saharan Africa’s population is growing and urbanising rapidly, creating expectations that demand for protein will increase rapidly over the coming decades and triggering calls for further investment in the expansion and intensification of the region’s meat and dairy sector. However, growing disquiet over the environmental impacts of further expansion in livestock numbers, and growing sales of alternative protein products in the Global North, has raised questions about the future place of plant-based, insect and lab-grown proteins in African diets and food systems. This report examines financial investment in protein production in sub-Saharan Africa. It begins from the position that investors play an important role in shaping the development of diets and food systems because they are able to mobilise the financial resources required to develop new protein products, infrastructures and value chains, or to prevent their development by withholding investment. It therefore investigates which actors are financing the production in sub-Saharan Africa of: a) animal proteins such as meat, fish, eggs and dairy products; b) ‘protein crops’ such as beans, pulses and legumes; and c) processed ‘alternative proteins’ derived from plants, insects, microbes or animal cells grown in a tissue culture. Through analysing investment by state, philanthropic and private sector organisations – as well as multilateral financial institutions such as development banks – it aims to establish which protein sources and stages of the value chain are financed by different groups of investors and to explore the values and goals which shape their investment decisions. To this end, the report examines four questions: 1. Who is currently investing in protein production in sub-Saharan Africa? 2. What goals do these investors aim to achieve (or what sort of future do they seek to bring about) through making these investments? 3. Which protein sources and protein production systems do they finance? 4. What theory of change links their investment strategy to these goals? In addressing these questions, this report explores what sorts of protein production and provisioning systems different investor groups might be helping to bring into being in sub-Saharan Africa. It also considers what alternative possibilities might be marginalised due to a lack of investment. It thus seeks to understand whose priorities, preferences and visions for the future of food might be informing the changing place of protein in the region’s diets, economies and food systems.
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Houzer, Ella, and Ian Scoones. Are Livestock Always Bad for the Planet? Rethinking the Protein Transition and Climate Change Debate. Institute of Development Studies (IDS), September 2021. http://dx.doi.org/10.19088/steps.2021.003.

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Urgent climate challenges have triggered calls for radical, widespread changes in what we eat, pushing for the drastic reduction if not elimination of animal-source foods from our diets. But high-profile debates, based on patchy evidence, are failing to differentiate between varied landscapes, environments and production methods. Relatively low-impact, extensive livestock production, such as pastoralism, is being lumped in with industrial systems in the conversation about the future of food. This report warns that the dominant picture of livestock’s impacts on climate change has been distorted by faulty assumptions that focus on intensive, industrial farming in rich countries. Millions of people worldwide who depend on extensive livestock production, with relatively lower climate impacts, are being ignored by debates on the future of food. The report identifies ten flaws in the way that livestock’s climate impacts have been assessed, and suggests how pastoralists could be better included in future debates about food and the climate.
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Dubcovsky, Jorge, Tzion Fahima, and Ann Blechl. Positional cloning of a gene responsible for high grain protein content in tetraploid wheat. United States Department of Agriculture, September 2003. http://dx.doi.org/10.32747/2003.7695875.bard.

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High Grain Protein Content (GPC) is a desirable trait in breadmaking and pasta wheat varieties because of its positive effects on quality and nutritional value. However, selection for GPC is limited by our poor understanding of the genes involved in the accumulation of protein in the grain. The long-term goal of this project is to provide a better understanding of the genes controlling GPC in wheat. The specific objectives of this project were: a) to develop a high-density genetic map of the GPC gene in tetraploid wheat, b) to construct a T. turgidum Bacterial Artificial Chromosome (BAC) library, c) to construct a physical map of the GPC gene and identify a candidate for the GPC gene. A gene with a large effect on GPC was detected in Triticum turgidum var. dicoccoides and was previously mapped in the short arm of chromosome 6B. To define better the position of the Gpc-B1 locus we developed homozygous recombinant lines with recombination events within the QTL region. Except for the 30-cM region of the QTL these RSLs were isogenic for the rest of the genome minimizing the genetic variability. To minimize the environmental variability the RSLs were characterized using 10 replications in field experiments organized in a Randomized Complete Block Design, which were repeated three times. Using this strategy, we were able to map this QTL as a single Mendelian locus (Gpc-B1) on a 2.6-cM region flanked by RFLP markers Xcdo365 and Xucw67. All three experiments showed that the lines carrying the DIC allele had an average absolute increase in GPC of 14 g/kg. Using the RFLP flanking markers, we established the microcolinearity between a 2.l-cM region including the Gpc-B1 gene in wheat chromosome 6BS and a 350-kb region on rice chromosome 2. Rice genes from this region were used to screen the Triticeae EST collection, and these ESTs were used to saturate the Gpc-B1 region with molecular markers. With these new markers we were able to map the Gpc-B1 locus within a 0.3-cM region flanked by PCR markers Xucw83 and Xucw71. These flanking markers defined a 36-kb colinear region with rice, including one gene that is a potential candidate for the Gpc-B1 gene. To develop a physical map of the Gpc-B1 region in wheat we first constructed a BAC library of tetraploid wheat, from RSL#65 including the high Gpc-B1 allele. We generated half- million clones with an average size of l3l-kb (5.1 X genome equivalents for each of the two genomes). This coverage provides a 99.4% probability of recovering any gene from durum wheat. We used the Gpc-BI flanking markers to screen this BAC library and then completed the physical map by chromosome walking. The physical map included two overlapping BACs covering a region of approximately 250-kb, including two flanking markers and the Gpc-B1 gene. Efforts are underway to sequence these two BACs to determine if additional wheat genes are present in this region. Weare also developing new RSLs to further dissect this region. We developed PCR markers for flanking loci Xucw79andXucw71 to facilitate the introgression of this gene in commercial varieties by marker assisted selection (httQ://maswheat.ucdavis.edu/ orotocols/HGPC/index.hlm). Using these markers we introgressed the Gpc-B1 gene in numerous pasta and common wheat breeding lines.
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Weinberg, Zwi G., Adegbola Adesogan, Itzhak Mizrahi, Shlomo Sela, Kwnag Jeong, and Diwakar Vyas. effect of selected lactic acid bacteria on the microbial composition and on the survival of pathogens in the rumen in context with their probiotic effects on ruminants. United States Department of Agriculture, January 2014. http://dx.doi.org/10.32747/2014.7598162.bard.

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This research project was performed in context of the apparent probiotic effect of selected lactic acid bacteria (LAB) silage inoculants on the performance of ruminants (improved feed intake, faster live-weight gain, higher milk yields and improved feed efficiency). The overall objective was to find out how LAB affect ruminant performance. The project included several “chapters” as follows: 1. The effect of LAB silage inoculants on the survival of detrimental bacteria in rumen fluid, in vitro study (Weinberg et al., The Volcani Center). An in vitro model was developed to study the interaction between selected LAB and an E. coli strain tagged with green fluorescence protein (GFP) in buffered RF. Results indicated that both LAB inoculants and E. coli survived in the RF for several days; both LAB inoculants and LAB-treated silages did not affect survival of E. coli in rumen fluid in vitro. The effect of feeding baled wheat silages treated with or without three selected LAB silage inoculants on the performance of high-lactating cows (Weinberg et al., The Volcani Center). Treatments included control (no additive), Lacobacillusbuchneri40788 (LB), Lactobacillus plantarumMTD1 40027 (LP) and Pediococcuspentosaceus30168 (PP), each applied at 10⁶ cfu/g FM. The silages were included in the TMR of 32 high milking Holstein cows in a controlled feeding experiment. All baled silages were of good quality. The LB silage had the numerically highest acetic acid and were the most stable upon aerobic exposure. The cows fed the LB silages had the highest daily milk yields, percent milk fat and protein. The microbiome of baled wheat silages and changes during ensiling of wheat and corn (Sela et al., The Volcani Center). Bacterial community of the baled silages was dominated mainly of two genera in total, dominated by Lactobacillus and Clostridium_sensu_stricto_12 with 300 other genera at very low abundance. Fungal community was composed mainly of two genera in total, dominated by Candida and Monascuswith 20 other genera at very low abundance. In addition, changes in the microbiome during ensiling of wheat and corn with and without addition of L. plantarumMTD1 was studied in mini-silos. Overall 236 bacterial genera were identified in the fresh corn but after 3 months Lactobacillus outnumbered all other species by acquiring 95% of relative abundance. The wheat silage samples are still under analysis. The effect of applying LAB inoculants at ensiling on survival of E. coli O157:H7 in alfalfa and corn silages(Adesogan et al., University of Florida). E. coli (10⁵ cfu/g) was applied to fresh alfalfa and corn at ensiling with or without L. plantarumor L. buchneri. The pathogen was added again after about 3 moths at the beginning of an aerobic exposure period. The inoculants resulted in faster decrease in pH as compared with the control (no additives) or E. coli alone and therefore, the pathogen was eliminated faster from these silages. After aerobic exposure the pathogen was not detected in the LAB treated silages, whereas it was still present in the E. coli alone samples. 5. The effect of feeding corn silage treated with or without L. buchnerion shedding of E. coli O157:H7 by dairy cows (Adesogan et al., UFL). BARD Report - Project 4704 Page 2 of 12 Five hundred cows from the dairy herd of the University of Florida were screened for E. coli shedding, out of which 14 low and 13 high shedders were selected. These cows were fed a total mixed ration (TMR) which was inoculated with E. coli O157:H7 for 21 days. The TMR included corn silage treated with or without L. buchneri. The inoculated silages were more stable upon aerobic exposure than the control silages; the silage inoculant had no significant effect on any milk or cow blood parameters. However, the silage inoculant tended to reduce shedding of E. coli regardless of high or low shedders (p = 0.06). 6. The effect of feeding baled wheat silages treated with or without three selected LAB silage inoculants on the rumen microbiome (Mizrahi et al., BGU). Rumen fluid was sampled throughout the feeding experiment in which inoculated wheat silages were included in the rations. Microbial DNA was subsequently purified from each sample and the 16S rRNA was sequenced, thus obtaining an overview of the microbiome and its dynamic changes for each experimental treatment. We observed an increase in OTU richness in the group which received the baled silage inoculated with Lactobacillus Plantarum(LP). In contrast the group fed Lactobacillus buchneri(LB) inoculated silage resulted in a significant decrease in richness. Lower OTU richness was recently associated in lactating cows with higher performance (Ben Shabatet al., 2016). No significant clustering could be observed between the different inoculation treatments and the control in non metric multi-dimentional scaling, suggesting that the effect of the treatments is not the result of an overall modulation of the microbiome composition but possibly the result of more discrete interactions. Significant phylum level changes in composition also indicates that no broad changes in taxa identity and composition occurred under any treatment A more discrete modulation could be observed in the fold change of several taxonomic groups (genus level analysis), unique to each treatment, before and after the treatment. Of particular interest is the LB treated group, in which several taxa significantly decreased in abundance. BARD Report - Project 4704 Page 3 of 12
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Anderson, Olin, Gad Galili, and Ann Blechl. Heterologous Expression of Wheat High Molecular Weight Glutenin Subunit Genes: Analysis and Modification of Protein Sequences Affecting Dough Quality. United States Department of Agriculture, January 1993. http://dx.doi.org/10.32747/1993.7603826.bard.

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Dubcovsky, Jorge, Tzion Fahima, and Ann Blechl. Molecular characterization and deployment of the high-temperature adult plant stripe rust resistance gene Yr36 from wheat. United States Department of Agriculture, November 2013. http://dx.doi.org/10.32747/2013.7699860.bard.

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Stripe rust, caused by Puccinia striiformis f. sp. tritici is one of the most destructive fungal diseases of wheat. Virulent races that appeared within the last decade caused drastic cuts in yields. The incorporation of genetic resistance against this pathogen is the most cost-effective and environmentally friendly solution to this problem. However, race specific seedling resistance genes provide only a temporary solution because fungal populations rapidly evolve to overcome this type of resistance. In contrast, high temperature adult plant (HTAP) resistance genes provide a broad spectrum resistance that is partial and more durable. The cloning of the first wheat HTAP stripe rust resistance gene Yr36 (Science 2009, 323:1357), funded by our previous (2007-2010) BARD grant, provided us for the first time with an entry point for understanding the mechanism of broad spectrum resistance. Two paralogous copies of this gene are tightly linked at the Yr36 locus (WKS1 and WKS2). The main objectives of the current study were to characterize the Yr36 (WKS) resistance mechanism and to identify and characterize alternative WKSgenes in wheat and wild relatives. We report here that the protein coded by Yr36, designated WKS1, that has a novel architecture with a functional kinase and a lipid binding START domain, is localized to chloroplast. Our results suggest that the presence of the START domain may affect the kinase activity. We have found that the WKS1 was over-expressed on leaf necrosis in wheat transgenic plants. When the isolated WKS1.1 splice variant transcript was transformed into susceptible wheat it conferred resistance to stripe rust, but the truncated variant WKS1.2 did not confer resistance. WKS1.1 and WKS1.2 showed different lipid binding profiling. WKS1.1 enters the chloroplast membrane, while WKS1.2 is only attached outside of the chloroplast membrane. The ascorbate peroxidase (APX) activity of the recombinant protein of TmtAPXwas found to be reduced by WKS1.1 protein in vitro. The WKS1.1 mature protein in the chloroplast is able to phosphorylate TmtAPXprotein in vivo. WKS1.1 induced cell death by suppressing APX activity and reducing the ability of the cell to detoxify reactive oxygen. The decrease of APX activity reduces the ability of the plant to detoxify the reactive H2O2 and is the possible mechanism underlying the accelerated cell death observed in the transgenic plants overexpressing WKS1.1 and in the regions surrounding a stripe rust infection in the wheat plants carrying the natural WKS1.1 gene. WKS2 is a nonfunctional paralog of WKS1 in wild emmer wheat, probably due to a retrotransposon insertion close to the alternative splicing site. In some other wild relatives of wheat, such as Aegilops comosa, there is only one copy of this gene, highly similar to WKS2, which is lucking the retrotransposon insertion. WKS2 gene present in wheat and WKS2-Ae from A. showed a different pattern of alternative splice variants, regardless of the presence of the retrotransposon insertion. Susceptible Bobwhite transformed with WKS2-Ae (without retrotansposon insertion in intron10), which derived from Aegilops comosaconferred resistance to stripe rust in wheat. The expression of WKS2-Ae in transgenic plants is up-regulated by temperature and pathogen infection. Combination of WKS1 and WKS2-Ae shows improved stripe rust resistance in WKS1×WKS2-Ae F1 hybrid plants. The obtained results show that WKS1 protein is accelerating programmed cell death observed in the regions surrounding a stripe rust infection in the wheat plants carrying the natural or transgenic WKS1 gene. Furthermore, characterization of the epistatic interactions of Yr36 and Yr18 demonstrated that these two genes have additive effects and can therefore be combined to increase partial resistance to this devastating pathogen of wheat. These achievements may have a broad impact on wheat breeding efforts attempting to protect wheat yields against one of the most devastating wheat pathogen.
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Anderson, Olin D., Gad Galili, and Ann E. Blechl. Enhancement of Essential Amino Acids in Cereal Seeds: Four Approaches to Increased Lysine Content. United States Department of Agriculture, October 1998. http://dx.doi.org/10.32747/1998.7585192.bard.

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Cereal seeds are the basis of the human diet, and their amino acid composition is thus of major nutritional and economic importance. Currently, deficiencies in essential amino acids are addressed, when possible, by additionalprotein sources or by supplementing animal feed with non-cereal protein or synthetic amino acids. A number of strategies have been suggested to make cereal flours more complete and balanced sources of amino acids, although systematic examination of such strategies is rare. This project proposed to begin such a systematic examination using four complementary and parallel approaches to increasing wheat seed lysine: 1) Modifying endogenous wheat seed proteins for increased lysine composition. 2) Overexpression of naturally occurring high-lysine proteins in the wheat endosperm. 3) Ectopic expression of proteins in the wheat endosperm. 4) Alteration of free lysine levels in the wheat endosperm. The results of these studies are expected to be wheat lines with increased lysine content and will establish a clearer understanding of the approaches most likely to enhance cereal seed protein quality. Progress is reported for all four objectives, with a significant foundation for further work on two of the objectives (modification of wheat storage proteins and lysine metabolism). Plans for continuing work on all four objectives are briefly outlined.
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Feldman, Moshe, Eitan Millet, Calvin O. Qualset, and Patrick E. McGuire. Mapping and Tagging by DNA Markers of Wild Emmer Alleles that Improve Quantitative Traits in Common Wheat. United States Department of Agriculture, February 2001. http://dx.doi.org/10.32747/2001.7573081.bard.

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The general goal was to identify, map, and tag, with DNA markers, segments of chromosomes of a wild species (wild emmer wheat, the progenitor of cultivated wheat) determining the number, chromosomal locations, interactions, and effects of genes that control quantitative traits when transferred to a cultivated plant (bread wheat). Slight modifications were introduced and not all objectives could be completed within the human and financial resources available, as noted with the specific objectives listed below: 1. To identify the genetic contribution of each of the available wild emmer chromosome-arm substitution lines (CASLs) in the bread wheat cultivar Bethlehem for quantitative traits, including grain yield and its components and grain protein concentration and yield, and the effect of major loci affecting the quality of end-use products. [The quality of end-use products was not analyzed.] 2. To determine the extent and nature of genetic interactions (epistatic effects) between and within homoeologous groups 1 and 7 for the chromosome arms carrying "wild" and "cultivated" alleles as expressed in grain and protein yields and other quantitative traits. [Two experiments were successful, grain protein concentration could not be measured; data are partially analyzed.] 3. To derive recombinant substitution lines (RSLs) for the chromosome arms of homoeologous groups 1 and 7 that were found previously to promote grain and protein yields of cultivated wheat. [The selection of groups 1 and 7 tons based on grain yield in pot experiments. After project began, it was decided also to derive RSLs for the available arms of homoeologous group 4 (4AS and 4BL), based on the apparent importance of chromosome group 4, based on early field trials of the CASLs.] 4. To characterize the RSLs for quantitative traits as in objective 1 and map and tag chromosome segments producing significant effects (quantitative trait loci, QTLs by RFLP markers. [Producing a large population of RSLs for each chromosome arm and mapping them proved more difficult than anticipated, low numbers of RSLs were obtained for two of the chromosome arms.] 5. To construct recombination genetic maps of chromosomes of homoeologous groups 1 and 7 and to compare them to existing maps of wheat and other cereals [Genetic maps are not complete for homoeologous groups 4 and 7.] The rationale for this project is that wild species have characteristics that would be valuable if transferred to a crop plant. We demonstrated the sequence of chromosome manipulations and genetic tests needed to confirm this potential value and enhance transfer. This research has shown that a wild tetraploid species harbors genetic variability for quantitative traits that is interactive and not simply additive when introduced into a common genetic background. Chromosomal segments from several chromosome arms improve yield and protein in wheat but their effect is presumably enhanced when combination of genes from several segments are integrated into a single genotype in order to achieve the benefits of genes from the wild species. The interaction between these genes and those in the recipient species must be accounted for. The results of this study provide a scientific basis for some of the disappointing results that have historically obtained when using wild species as donors for crop improvement and provide a strategy for further successes.
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