Log in

Relevant bibliographies by topics / HigBA type II TA system / Journal articles

To see the other types of publications on this topic, follow the link: HigBA type II TA system.

Journal articles on the topic 'HigBA type II TA system'

Author: Grafiati

Published: 6 September 2023

Last updated: 7 September 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'HigBA type II TA system.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Jadhav, Pankaj Vilas, Vikrant Kumar Sinha, Saurabh Chugh, et al. "2.09 Å Resolution structure of E. coli HigBA toxin–antitoxin complex reveals an ordered DNA-binding domain and intrinsic dynamics in antitoxin." Biochemical Journal 477, no. 20 (2020): 4001–19. http://dx.doi.org/10.1042/bcj20200363.

Full text

Abstract:

The toxin–antitoxin (TA) systems are small operon systems that are involved in important physiological processes in bacteria such as stress response and persister cell formation. Escherichia coli HigBA complex belongs to the type II TA systems and consists of a protein toxin called HigB and a protein antitoxin called HigA. The toxin HigB is a ribosome-dependent endoribonuclease that cleaves the translating mRNAs at the ribosome A site. The antitoxin HigA directly binds the toxin HigB, rendering the HigBA complex catalytically inactive. The existing biochemical and structural studies had reveal

APA, Harvard, Vancouver, ISO, and other styles

2

Norouzi, Masoumeh, Abbas Maleki, Elham Aboualigalehdari, and Sobhan Ghafourian. "Type II toxin- antitoxin systems in clinical isolates of antibiotic resistant Acinetobacter baumannii." Genetika 54, no. 2 (2022): 625–32. http://dx.doi.org/10.2298/gensr2202625n.

Full text

Abstract:

The over use of antibiotics to treat infections in humans and animals made a phenomenon of the antibiotic-resistant bacteria. While studies focused to find on new antibiotics but, identification of novel antibacterial targets in bacteria is very important. By Toxin antitoxin systems this hypothesis could be done, whereas by the activation of a toxin or inactivation of an antitoxin, the raised toxin kills the bacterium. These systems are attractive target for antimicrobial therapy. However, the most important step for potency of TA system, as an antibacterial target, is to identify a TA system

APA, Harvard, Vancouver, ISO, and other styles

3

Park, Jin-Young, Hyo Jung Kim, Chinar Pathak, et al. "Induced DNA bending by unique dimerization of HigA antitoxin." IUCrJ 7, no. 4 (2020): 748–60. http://dx.doi.org/10.1107/s2052252520006466.

Full text

Abstract:

The bacterial toxin–antitoxin (TA) system regulates cell growth under various environmental stresses. Mycobacterium tuberculosis, the causative pathogen of tuberculosis (TB), has three HigBA type II TA systems with reverse gene organization, consisting of the toxin protein HigB and labile antitoxin protein HigA. Most type II TA modules are transcriptionally autoregulated by the antitoxin itself. In this report, we first present the crystal structure of the M. tuberculosis HigA3 antitoxin (MtHigA3) and MtHigA3 bound to its operator DNA complex. We also investigated the interaction between MtHig

APA, Harvard, Vancouver, ISO, and other styles

4

Klimkaitė, Laurita, Julija Armalytė, Jūratė Skerniškytė, and Edita Sužiedėlienė. "The Toxin-Antitoxin Systems of the Opportunistic Pathogen Stenotrophomonas maltophilia of Environmental and Clinical Origin." Toxins 12, no. 10 (2020): 635. http://dx.doi.org/10.3390/toxins12100635.

Full text

Abstract:

Stenotrophomonas maltophilia is a ubiquitous environmental bacterium that has recently emerged as a multidrug-resistant opportunistic pathogen causing bloodstream, respiratory, and urinary tract infections. The connection between the commensal environmental S. maltophilia and the opportunistic pathogen strains is still under investigation. Bacterial toxin–antitoxin (TA) systems have been previously associated with pathogenic traits, such as biofilm formation and resistance to antibiotics, which are important in clinical settings. The same species of the bacterium can possess various sets of TA

APA, Harvard, Vancouver, ISO, and other styles

5

Habib, Gul, Qing Zhu, and Baolin Sun. "Bioinformatics and Functional Assessment of Toxin-Antitoxin Systems in Staphylococcus aureus." Toxins 10, no. 11 (2018): 473. http://dx.doi.org/10.3390/toxins10110473.

Full text

Abstract:

Staphylococcus aureus is a nosocomial pathogen that can cause chronic to persistent infections. Among different mediators of pathogenesis, toxin-antitoxin (TA) systems are emerging as the most prominent. These systems are frequently studied in Escherichia coli and Mycobacterial species but rarely explored in S. aureus. In the present study, we thoroughly analyzed the S. aureus genome and screened all possible TA systems using the Rasta bacteria and toxin-antitoxin database. We further searched E. coli and Mycobacterial TA homologs and selected 67 TA loci as putative TA systems in S. aureus. Th

APA, Harvard, Vancouver, ISO, and other styles

6

Fivian-Hughes, Amanda S., and Elaine O. Davis. "Analyzing the Regulatory Role of the HigA Antitoxin within Mycobacterium tuberculosis." Journal of Bacteriology 192, no. 17 (2010): 4348–56. http://dx.doi.org/10.1128/jb.00454-10.

Full text

Abstract:

ABSTRACT Bacterial chromosomally encoded type II toxin-antitoxin (TA) loci may be involved in survival upon exposure to stress and have been linked to persistence and dormancy. Therefore, understanding the role of the numerous predicted TA loci within the human pathogen Mycobacterium tuberculosis has become a topic of great interest. Antitoxin proteins are known to autoregulate TA expression under normal growth conditions, but it is unknown whether they have a more global role in transcriptional regulation. This study focuses on analyzing the regulatory role of the M. tuberculosis HigA antitox

APA, Harvard, Vancouver, ISO, and other styles

7

Kamruzzaman, Muhammad, Alma Y. Wu, and Jonathan R. Iredell. "Biological Functions of Type II Toxin-Antitoxin Systems in Bacteria." Microorganisms 9, no. 6 (2021): 1276. http://dx.doi.org/10.3390/microorganisms9061276.

Full text

Abstract:

After the first discovery in the 1980s in F-plasmids as a plasmid maintenance system, a myriad of toxin-antitoxin (TA) systems has been identified in bacterial chromosomes and mobile genetic elements (MGEs), including plasmids and bacteriophages. TA systems are small genetic modules that encode a toxin and its antidote and can be divided into seven types based on the nature of the antitoxin molecules and their mechanism of action to neutralise toxins. Among them, type II TA systems are widely distributed in chromosomes and plasmids and the best studied so far. Maintaining genetic material may

APA, Harvard, Vancouver, ISO, and other styles

8

Levante, Alessia, Camilla Lazzi, Giannis Vatsellas, et al. "Genome Sequencing of five Lacticaseibacillus Strains and Analysis of Type I and II Toxin-Antitoxin System Distribution." Microorganisms 9, no. 3 (2021): 648. http://dx.doi.org/10.3390/microorganisms9030648.

Full text

Abstract:

The analysis of bacterial genomes is a potent tool to investigate the distribution of specific traits related to the ability of surviving in particular environments. Among the traits associated with the adaptation to hostile conditions, toxin–antitoxin (TA) systems have recently gained attention in lactic acid bacteria. In this work, genome sequences of Lacticaseibacillus strains of dairy origin were compared, focusing on the distribution of type I TA systems homologous to Lpt/RNAII and of the most common type II TA systems. A high number of TA systems have been identified spread in all the an

APA, Harvard, Vancouver, ISO, and other styles

9

Kang, Sung-Min, Do-Hee Kim, Chenglong Jin, and Bong-Jin Lee. "A Systematic Overview of Type II and III Toxin-Antitoxin Systems with a Focus on Druggability." Toxins 10, no. 12 (2018): 515. http://dx.doi.org/10.3390/toxins10120515.

Full text

Abstract:

Toxin-antitoxin (TA) systems are known to play various roles in physiological processes, such as gene regulation, growth arrest and survival, in bacteria exposed to environmental stress. Type II TA systems comprise natural complexes consisting of protein toxins and antitoxins. Each toxin and antitoxin participates in distinct regulatory mechanisms depending on the type of TA system. Recently, peptides designed by mimicking the interfaces between TA complexes showed its potential to activate the activity of toxin by competing its binding counterparts. Type II TA systems occur more often in path

APA, Harvard, Vancouver, ISO, and other styles

10

Valizadeh, Nasrin, Firuzeh Valian, Nourkhoda Sadeghifard, et al. "The Role of Peganum harmala Ethanolic Extract and Type II Toxin Antitoxin System in Biofilm Formation." Drug Research 67, no. 07 (2017): 385–87. http://dx.doi.org/10.1055/s-0043-102060.

Full text

Abstract:

AbstractToxin antitoxin system is a regulatory system that antitoxin inhibits the toxin. We aimed to determine the role of TA loci in biofilm formation in K. pneumoniae clinical and environmental isolates; also inhibition of biofilm formation by Peganum harmala. So, 40 K. pneumoniae clinical and environmental isolates were subjected for PCR to determine the frequency of mazEF, relEB, and mqsRA TA loci. Biofilm formation assay subjected for all isolates. Then, P. harmala was tested against positive biofilm formation strains. Our results demonstrated that relBE TA loci were dominant TA loci; whe

APA, Harvard, Vancouver, ISO, and other styles

11

Lee, Ki-Young, and Bong-Jin Lee. "Dynamics-Based Regulatory Switches of Type II Antitoxins: Insights into New Antimicrobial Discovery." Antibiotics 12, no. 4 (2023): 637. http://dx.doi.org/10.3390/antibiotics12040637.

Full text

Abstract:

Type II toxin-antitoxin (TA) modules are prevalent in prokaryotes and are involved in cell maintenance and survival under harsh environmental conditions, including nutrient deficiency, antibiotic treatment, and human immune responses. Typically, the type II TA system consists of two protein components: a toxin that inhibits an essential cellular process and an antitoxin that neutralizes its toxicity. Antitoxins of type II TA modules typically contain the structured DNA-binding domain responsible for TA transcription repression and an intrinsically disordered region (IDR) at the C-terminus that

APA, Harvard, Vancouver, ISO, and other styles

12

Hosseini, Nava, Maryam Pourhajibagher, Nasim Chiniforush, Nazanin Hosseinkhan, Parizad Rezaie, and Abbas Bahador. "Modulation of Toxin-Antitoxin System Rnl AB Type II in Phage-Resistant Gammaproteobacteria Surviving Photodynamic Treatment." Journal of Lasers in Medical Sciences 10, no. 1 (2018): 21–28. http://dx.doi.org/10.15171/jlms.2019.03.

Full text

Abstract:

Type II toxin-antitoxin (TA) systems are the particular type of TA modules which take part in different kinds of cellular actions, such as biofilm formation, persistence, stress endurance, defense of the bacterial cell against multiple phage attacks, plasmid maintenance, and programmed cell death in favor of bacterial population. Although several bioinformatics and Pet lab studies have already been conducted to understand the functionality of already discovered TA systems, still, more work in this area is required. Rnl AB type II TA module, which is composed of RnlA toxin and RnlB antitoxin, i

APA, Harvard, Vancouver, ISO, and other styles

13

Alkhalili, Rawana, Joel Wallenius, and Björn Canbäck. "Towards Exploring Toxin-Antitoxin Systems in Geobacillus: A Screen for Type II Toxin-Antitoxin System Families in a Thermophilic Genus." International Journal of Molecular Sciences 20, no. 23 (2019): 5869. http://dx.doi.org/10.3390/ijms20235869.

Full text

Abstract:

The toxin-antitoxin (TA) systems have been attracting attention due to their role in regulating stress responses in prokaryotes and their biotechnological potential. Much recognition has been given to type II TA system of mesophiles, while thermophiles have received merely limited attention. Here, we are presenting the putative type II TA families encoded on the genomes of four Geobacillus strains. We employed the TA finder tool to mine for TA-coding genes and manually curated the results using protein domain analysis tools. We also used the NCBI BLAST, Operon Mapper, ProOpDB, and sequence ali

APA, Harvard, Vancouver, ISO, and other styles

14

Tu, Chih-Han, Michelle Holt, Shengfeng Ruan, and Christina Bourne. "Evaluating the Potential for Cross-Interactions of Antitoxins in Type II TA Systems." Toxins 12, no. 6 (2020): 422. http://dx.doi.org/10.3390/toxins12060422.

Full text

Abstract:

The diversity of Type-II toxin–antitoxin (TA) systems in bacterial genomes requires tightly controlled interaction specificity to ensure protection of the cell, and potentially to limit cross-talk between toxin–antitoxin pairs of the same family of TA systems. Further, there is a redundant use of toxin folds for different cellular targets and complexation with different classes of antitoxins, increasing the apparent requirement for the insulation of interactions. The presence of Type II TA systems has remained enigmatic with respect to potential benefits imparted to the host cells. In some cas

APA, Harvard, Vancouver, ISO, and other styles

15

Hosseini, Mandana, Jamileh Nowroozi, and Nour Amirmozafari. "The effect of type II toxin-antitoxin systems on methicillinresistant Staphylococcus aureus persister cell formation and antibiotic tolerance." Acta Biologica Szegediensis 65, no. 1 (2021): 113–17. http://dx.doi.org/10.14232/abs.2021.1.113-117.

Full text

Abstract:

Persister cells are defi ned as a subpopulation of bacteria in a dormant state with the ability to reduce bacterial metabolism and they are involved in antibiotic tolerance. Toxin-antitoxin (TA) systems have been previously suggested as important players in persistence. Therefore, this study aimed to study the involvement of TA systems in persister cell formation in methicillin-resistant Staphylococcus aureus following antibiotic exposure. Using TADB and RASTA database, two type II TA systems including MazF/MazE and RelE/RelB were predicted in S. aureus. The presence of these TA genes was dete

APA, Harvard, Vancouver, ISO, and other styles

16

Xue, Lu, Jian Yue, Jiyuan Ke, et al. "Distinct oligomeric structures of the YoeB–YefM complex provide insights into the conditional cooperativity of type II toxin–antitoxin system." Nucleic Acids Research 48, no. 18 (2020): 10527–41. http://dx.doi.org/10.1093/nar/gkaa706.

Full text

Abstract:

Abstract YoeB–YefM, the widespread type II toxin–antitoxin (TA) module, binds to its own promoter to autoregulate its transcription: repress or induce transcription under normal or stress conditions, respectively. It remains unclear how YoeB–YefM regulates its transcription depending on the YoeB to YefM TA ratio. We find that YoeB–YefM complex from S.aureus exists as two distinct oligomeric assemblies: heterotetramer (YoeB–YefM2–YoeB) and heterohexamer (YoeB–YefM2–YefM2–YoeB) with low and high DNA-binding affinities, respectively. Structures of the heterotetramer alone and heterohexamer bound

APA, Harvard, Vancouver, ISO, and other styles

17

Gómez, Leonardo A., Raúl E. Molina, Rodrigo I. Soto, et al. "Unraveling the Role of the Zinc-Dependent Metalloproteinase/HTH-Xre Toxin/Antitoxin (TA) System of Brucella abortus in the Oxidative Stress Response: Insights into the Stress Response and Virulence." Toxins 15, no. 9 (2023): 536. http://dx.doi.org/10.3390/toxins15090536.

Full text

Abstract:

Toxin/antitoxin (TA) systems have been scarcely studied in Brucella abortus, the causative agent of brucellosis, which is one of the most prevalent zoonotic diseases worldwide. In this study, the roles of a putative type II TA system composed by a Zinc-dependent metalloproteinase (ZnMP) and a transcriptional regulator HTH-Xre were evaluated. The deletion of the open reading frame (ORF) BAB1_0270, coding for ZnMP, used to produce a mutant strain, allowed us to evaluate the survival and gene expression of B. abortus 2308 under oxidative conditions. Our results showed that the B. abortus mutant s

APA, Harvard, Vancouver, ISO, and other styles

18

Ni, Songwei, Baiyuan Li, Kaihao Tang, et al. "Conjugative plasmid-encoded toxin–antitoxin system PrpT/PrpA directly controls plasmid copy number." Proceedings of the National Academy of Sciences 118, no. 4 (2021): e2011577118. http://dx.doi.org/10.1073/pnas.2011577118.

Full text

Abstract:

Toxin–antitoxin (TA) loci were initially identified on conjugative plasmids, and one function of plasmid-encoded TA systems is to stabilize plasmids or increase plasmid competition via postsegregational killing. Here, we discovered that the type II TA system, Pseudoalteromonas rubra plasmid toxin–antitoxin PrpT/PrpA, on a low-copy-number conjugative plasmid, directly controls plasmid replication. Toxin PrpT resembles ParE of plasmid RK2 while antitoxin PrpA (PF03693) shares no similarity with previously characterized antitoxins. Surprisingly, deleting this prpA-prpT operon from the plasmid doe

APA, Harvard, Vancouver, ISO, and other styles

19

Zhou, Jingyi, Shouyi Li, Haozhou Li, et al. "Identification of a Toxin–Antitoxin System That Contributes to Persister Formation by Reducing NAD in Pseudomonas aeruginosa." Microorganisms 9, no. 4 (2021): 753. http://dx.doi.org/10.3390/microorganisms9040753.

Full text

Abstract:

Bacterial persisters are slow-growing or dormant cells that are highly tolerant to bactericidal antibiotics and contribute to recalcitrant and chronic infections. Toxin/antitoxin (TA) systems play important roles in controlling persister formation. Here, we examined the roles of seven predicted type II TA systems in the persister formation of a Pseudomonas aeruginosa wild-type strain PA14. Overexpression of a toxin gene PA14_51010 or deletion of the cognate antitoxin gene PA14_51020 increased the bacterial tolerance to antibiotics. Co-overexpression of PA14_51010 and PA14_51020 or simultaneous

APA, Harvard, Vancouver, ISO, and other styles

20

Bajaj, R. Alexandra, Mark A. Arbing, Annie Shin, Duilio Cascio, and Linda Miallau. "Crystal structure of the toxin Msmeg_6760, the structural homolog ofMycobacterium tuberculosisRv2035, a novel type II toxin involved in the hypoxic response." Acta Crystallographica Section F Structural Biology Communications 72, no. 12 (2016): 863–69. http://dx.doi.org/10.1107/s2053230x16017957.

Full text

Abstract:

The structure of Msmeg_6760, a protein of unknown function, has been determined. Biochemical and bioinformatics analyses determined that Msmeg_6760 interacts with a protein encoded in the same operon, Msmeg_6762, and predicted that the operon is a toxin–antitoxin (TA) system. Structural comparison of Msmeg_6760 with proteins of known function suggests that Msmeg_6760 binds a hydrophobic ligand in a buried cavity lined by large hydrophobic residues. Access to this cavity could be controlled by a gate–latch mechanism. The function of the Msmeg_6760 toxin is unknown, but structure-based predictio

APA, Harvard, Vancouver, ISO, and other styles

21

Tasneem, Maisha, Shipan Das Gupta, Monira Binte Momin, Kazi Modasser Hossain, Tasnim Binta Osman, and Md Fazley Rabbi. "In silico annotation of a hypothetical protein from Listeria monocytogenes EGD-e unfolds a toxin protein of the type II secretion system." Genomics & Informatics 21, no. 1 (2023): e7. http://dx.doi.org/10.5808/gi.22071.

Full text

Abstract:

The gram-positive bacterium Listeria monocytogenes is an important foodborne intracellular pathogen that is widespread in the environment. The functions of hypothetical proteins (HP) from various pathogenic bacteria have been successfully annotated using a variety of bioinformatics strategies. In this study, a HP Imo0888 (NP_464414.1) from the Listeria monocytogenes EGD-e strain was annotated using several bioinformatics tools. Various techniques, including CELLO, PSORTb, and SOSUIGramN, identified the candidate protein as cytoplasmic. Domain and motif analysis revealed that the target protein

APA, Harvard, Vancouver, ISO, and other styles

22

Vogelgsang, Lars, Azlan Nisar, Sebastian Alexander Scharf, et al. "Characterisation of Type II DNA Methyltransferases of Metamycoplasma hominis." Microorganisms 11, no. 6 (2023): 1591. http://dx.doi.org/10.3390/microorganisms11061591.

Full text

Abstract:

Bacterial virulence, persistence and defence are affected by epigenetic modifications, including DNA methylation. Solitary DNA methyltransferases modulate a variety of cellular processes and influence bacterial virulence; as part of a restriction-modification (RM) system, they act as a primitive immune system in methylating the own DNA, while unmethylated foreign DNA is restricted. We identified a large family of type II DNA methyltransferases in Metamycoplasma hominis, comprising six solitary methyltransferases and four RM systems. Motif-specific 5mC and 6mA methylations were identified with

APA, Harvard, Vancouver, ISO, and other styles

23

Heaton, Brook E., Julien Herrou, Anne E. Blackwell, Vicki H. Wysocki, and Sean Crosson. "Molecular Structure and Function of the Novel BrnT/BrnA Toxin-Antitoxin System of Brucella abortus." Journal of Biological Chemistry 287, no. 15 (2012): 12098–110. http://dx.doi.org/10.1074/jbc.m111.332163.

Full text

Abstract:

Type II toxin-antitoxin (TA) systems are expressed from two-gene operons that encode a cytoplasmic protein toxin and its cognate protein antitoxin. These gene cassettes are often present in multiple copies on bacterial chromosomes, where they have been reported to regulate stress adaptation and persistence during antimicrobial treatment. We have identified a novel type II TA cassette in the intracellular pathogen Brucella abortus that consists of the toxin gene, brnT, and its antitoxin, brnA. BrnT is coexpressed and forms a 2:2 tetrameric complex with BrnA, which neutralizes BrnT toxicity. The

APA, Harvard, Vancouver, ISO, and other styles

24

Kędzierska, Barbara, and Katarzyna Potrykus. "Minigene as a Novel Regulatory Element in Toxin-Antitoxin Systems." International Journal of Molecular Sciences 22, no. 24 (2021): 13389. http://dx.doi.org/10.3390/ijms222413389.

Full text

Abstract:

The axe-txe type II toxin-antitoxin (TA) system is characterized by a complex and multilayered mode of gene expression regulation. Precise and tight control of this process is crucial to keep the toxin in an appropriate balance with the cognate antitoxin until its activation is needed for the cell. In this report, we provide evidence that a minigene encoded within the axe-txe operon influences translation of the Txe toxin. This is the first example to date of such a regulatory mechanism identified in the TA modules. Here, in a series of genetic studies, we employed translational reporter gene

APA, Harvard, Vancouver, ISO, and other styles

25

Rathore, Jitendra Singh, and Lalit Kumar Gautam. "Expression, Purification, and Functional Analysis of Novel RelE Operon fromX. nematophila." Scientific World Journal 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/428159.

Full text

Abstract:

Bacterial toxin-antitoxin (TA) complexes induce programmed cell death and also function to relieve cell from stress by various response mechanisms.Escherichia coliRelB-RelE TA complex consists of a RelE toxin functionally counteracted by RelB antitoxin. In the present study, a novel homolog of RelE toxin designated as Xn-relE toxin fromXenorhabdus nematophilapossessing its own antitoxin designated as Xn-relEAT has been identified. Expression and purification of recombinant proteins under native conditions with GST and Ni-NTA chromatography prove the existence of novel TA module. The expression

APA, Harvard, Vancouver, ISO, and other styles

26

Choi, Wonho, Yoshihiro Yamaguchi, Ji-Young Park, et al. "Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens." Microorganisms 9, no. 5 (2021): 1107. http://dx.doi.org/10.3390/microorganisms9051107.

Full text

Abstract:

Agrobacterium tumefaciens is a pathogen of various plants which transfers its own DNA (T-DNA) to the host plants. It is used for producing genetically modified plants with this ability. To control T-DNA transfer to the right place, toxin-antitoxin (TA) systems of A. tumefaciens were used to control the target site of transfer without any unintentional targeting. Here, we describe a toxin-antitoxin system, Atu0939 (mazE-at) and Atu0940 (mazF-at), in the chromosome of Agrobacterium tumefaciens. The toxin in the TA system has 33.3% identity and 45.5% similarity with MazF in Escherichia coli. The

APA, Harvard, Vancouver, ISO, and other styles

27

Jin, Chenglong, Sung-Min Kang, Do-Hee Kim, and Bong-Jin Lee. "Structural and functional analysis of the Klebsiella pneumoniae MazEF toxin–antitoxin system." IUCrJ 8, no. 3 (2021): 362–71. http://dx.doi.org/10.1107/s2052252521000452.

Full text

Abstract:

Bacterial toxin–antitoxin (TA) systems correlate strongly with physiological processes in bacteria, such as growth arrest, survival and apoptosis. Here, the first crystal structure of a type II TA complex structure of Klebsiella pneumoniae at 2.3 Å resolution is presented. The K. pneumoniae MazEF complex consists of two MazEs and four MazFs in a heterohexameric assembly. It was estimated that MazEF forms a dodecamer with two heterohexameric MazEF complexes in solution, and a truncated complex exists in heterohexameric form. The MazE antitoxin interacts with the MazF toxin via two binding modes

APA, Harvard, Vancouver, ISO, and other styles

28

Dong, Jinggang, Hanjie Gu, Huiqin Huang, Xiaoqian Tang, and Yonghua Hu. "Small RNA sR158 Participates in Oxidation Stress Tolerance and Pathogenicity of Edwardaiella piscicida by Regulating TA System YefM-YoeB." Aquaculture Research 2023 (May 16, 2023): 1–8. http://dx.doi.org/10.1155/2023/9967821.

Full text

Abstract:

In recent years, the role of bacterial sRNAs in adversity tolerance and pathogens has attracted increasing attention. A great number of virulence-related sRNAs were reported in a variety of human pathogens. However, only a few sRNAs from aquatic pathogens were reported. In our previous study, a novel sRNA, sR158, was identified in Edwardsiella piscicida, an important aquatic pathogen, but its function remains unknown. In the same aquatic pathogen, we also identified a type II TA system, YefM-YoeB, in another study. In the current report, we found that the expression of yefM-yoeB in E. piscicid

APA, Harvard, Vancouver, ISO, and other styles

29

Zhou, Juan, Xue-Jian Du, Ying Liu, et al. "Insights into the Neutralization and DNA Binding of Toxin–Antitoxin System ParESO-CopASO by Structure-Function Studies." Microorganisms 9, no. 12 (2021): 2506. http://dx.doi.org/10.3390/microorganisms9122506.

Full text

Abstract:

ParESO-CopASO is a new type II toxin–antitoxin (TA) system in prophage CP4So that plays an essential role in circular CP4So maintenance after the excision in Shewanella oneidensis. The toxin ParESO severely inhibits cell growth, while CopASO functions as an antitoxin to neutralize ParESO toxicity through direct interactions. However, the molecular mechanism of the neutralization and autoregulation of the TA operon transcription remains elusive. In this study, we determined the crystal structure of a ParESO-CopASO complex that adopted an open V-shaped heterotetramer with the organization of Par

APA, Harvard, Vancouver, ISO, and other styles

30

Sofiev, M., R. Vankevich, M. Lanne, et al. "An operational system for the assimilation of satellite information on wild-land fires for the needs of air quality modelling and forecasting." Atmospheric Chemistry and Physics Discussions 9, no. 2 (2009): 6483–513. http://dx.doi.org/10.5194/acpd-9-6483-2009.

Full text

Abstract:

Abstract. This paper investigates a potential of two remotely sensed wild-land fire characteristics: 4-μm Brightness Temperature Anomaly (TA) and Fire Radiative Power (FRP) for the needs of operational chemical transport modelling and the short-term forecasting of the atmospheric composition and air quality. Two treatments of the TA and FRP data are presented and a methodology for evaluating the emission fluxes is described. The method does not contain a complicated analysis of vegetation state, fuel load, burning efficiency and related factors, which are comparatively uncertain but inevitably

APA, Harvard, Vancouver, ISO, and other styles

31

Kang, Sung-Min, Ji Sung Koo, Chang-Min Kim, Do-Hee Kim, and Bong-Jin Lee. "mRNA Interferase Bacillus cereus BC0266 Shows MazF-Like Characteristics Through Structural and Functional Study." Toxins 12, no. 6 (2020): 380. http://dx.doi.org/10.3390/toxins12060380.

Full text

Abstract:

Toxin–antitoxin (TA) systems are prevalent in bacteria and are known to regulate cellular growth in response to stress. As various functions related to TA systems have been revealed, the importance of TA systems are rapidly emerging. Here, we present the crystal structure of putative mRNA interferase BC0266 and report it as a type II toxin MazF. The MazF toxin is a ribonuclease activated upon and during stressful conditions, in which it cleaves mRNA in a sequence-specific, ribosome-independent manner. Its prolonged activity causes toxic consequences to the bacteria which, in turn, may lead to

APA, Harvard, Vancouver, ISO, and other styles

32

Nishimura, Motoko, Shigeharu Uchida, Shigeki Mitsunaga та ін. "Characterization of T-Cell Clones Derived From Peripheral Blood Lymphocytes of a Patient With Transfusion-Associated Graft-Versus-Host Disease: Fas-Mediated Killing by CD4+ and CD8+ Cytotoxic T-Cell Clones and Tumor Necrosis Factor β Production by CD4+ T-Cell Clones". Blood 89, № 4 (1997): 1440–45. http://dx.doi.org/10.1182/blood.v89.4.1440.

Full text

Abstract:

Abstract Transfusion-associated graft-versus-host disease (TA-GVHD) is one of the most serious adverse effects of blood transfusion. It is generally thought to be caused by the infused lymphocytes. Donor-derived cytotoxic T lymphocytes (CTLs) directed against the recipient's HLAs, which have escaped the recipient's immune system and are proliferating, are considered to attack recipient organs and tissues. Despite the seriousness of the disease, the precise mechanism of its development remains unclear and no definitive treatment has been developed. With the aim of developing an effective treatm

APA, Harvard, Vancouver, ISO, and other styles

33

Bleriot, Ines, Lucia Blasco, Mercedes Delgado-Valverde, et al. "Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK." Toxins 12, no. 9 (2020): 566. http://dx.doi.org/10.3390/toxins12090566.

Full text

Abstract:

Although the failure of antibiotic treatment is normally attributed to resistance, tolerance and persistence display a significant role in the lack of response to antibiotics. Due to the fact that several nosocomial pathogens show a high level of tolerance and/or resistance to chlorhexidine, in this study we analyzed the molecular mechanisms associated with chlorhexidine adaptation in two clinical strains of Klebsiella pneumoniae by phenotypic and transcriptomic studies. These two strains belong to ST258-KPC3 (high-risk clone carrying β-lactamase KPC3) and ST846-OXA48 (low-risk clone carrying

APA, Harvard, Vancouver, ISO, and other styles

34

Lyman, M. G., C. D. Kemp, M. P. Taylor, and L. W. Enquist. "Comparison of the Pseudorabies Virus Us9 Protein with Homologs from Other Veterinary and Human Alphaherpesviruses." Journal of Virology 83, no. 14 (2009): 6978–86. http://dx.doi.org/10.1128/jvi.00598-09.

Full text

Abstract:

ABSTRACT Pseudorabies virus (PRV) Us9 is a small, tail-anchored (TA) membrane protein that is essential for axonal sorting of viral structural proteins and is highly conserved among other members of the alphaherpesvirus subfamily. We cloned the Us9 homologs from two human pathogens, varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1), as well as two veterinary pathogens, equine herpesvirus type 1 (EHV-1) and bovine herpesvirus type 1 (BHV-1), and fused them to enhanced green fluorescent protein to examine their subcellular localization and membrane topology. Akin to PRV Us9, a

APA, Harvard, Vancouver, ISO, and other styles

35

Maggi, Stefano, Alberto Ferrari, Korotoum Yabre, Aleksandra Anna Bonini, Claudio Rivetti, and Claudia Folli. "Strategies to Investigate Membrane Damage, Nucleoid Condensation, and RNase Activity of Bacterial Toxin–Antitoxin Systems." Methods and Protocols 4, no. 4 (2021): 71. http://dx.doi.org/10.3390/mps4040071.

Full text

Abstract:

A large number of bacterial toxin–antitoxin (TA) systems have been identified so far and different experimental approaches have been explored to investigate their activity and regulation both in vivo and in vitro. Nonetheless, a common feature of these methods is represented by the difficulty in cell transformation, culturing, and stability of the transformants, due to the expression of highly toxic proteins. Recently, in dealing with the type I Lpt/RNAII and the type II YafQ/DinJ TA systems, we encountered several of these problems that urged us to optimize methodological strategies to study

APA, Harvard, Vancouver, ISO, and other styles

36

Ariyachaokun, Kanchiyaphat, Anna D. Grabowska, Claude Gutierrez, and Olivier Neyrolles. "Multi-Stress Induction of the Mycobacterium tuberculosis MbcTA Bactericidal Toxin-Antitoxin System." Toxins 12, no. 5 (2020): 329. http://dx.doi.org/10.3390/toxins12050329.

Full text

Abstract:

MbcTA is a type II toxin/antitoxin (TA) system of Mycobacterium tuberculosis. The MbcT toxin triggers mycobacterial cell death in vitro and in vivo through the phosphorolysis of the essential metabolite NAD+ and its bactericidal activity is neutralized by physical interaction with its cognate antitoxin MbcA. Therefore, the MbcTA system appears as a promising target for the development of novel therapies against tuberculosis, through the identification of compounds able to antagonize or destabilize the MbcA antitoxin. Here, the expression of the mbcAT operon and its regulation were investigated

APA, Harvard, Vancouver, ISO, and other styles

37

Jurėnas, Dukas, Laurence Van Melderen, and Abel Garcia-Pino. "Crystallization and X-ray analysis of all of the players in the autoregulation of theataRTtoxin–antitoxin system." Acta Crystallographica Section F Structural Biology Communications 74, no. 7 (2018): 391–401. http://dx.doi.org/10.1107/s2053230x18007914.

Full text

Abstract:

TheataRToperon from enteropathogenicEscherichia coliencodes a toxin–antitoxin (TA) module with a recently discovered novel toxin activity. This new type II TA module targets translation initiation for cell-growth arrest. Virtually nothing is known regarding the molecular mechanisms of neutralization, toxin catalytic action or translation autoregulation. Here, the production, biochemical analysis and crystallization of the intrinsically disordered antitoxin AtaR, the toxin AtaT, the AtaR–AtaT complex and the complex of AtaR–AtaT with a double-stranded DNA fragment of the operator region of the

APA, Harvard, Vancouver, ISO, and other styles

38

Kim, Do-Hee, Sung-Min Kang, Sung-Min Baek, et al. "Role of PemI in the Staphylococcus aureus PemIK toxin–antitoxin complex: PemI controls PemK by acting as a PemK loop mimic." Nucleic Acids Research 50, no. 4 (2022): 2319–33. http://dx.doi.org/10.1093/nar/gkab1288.

Full text

Abstract:

Abstract Staphylococcus aureus is a notorious and globally distributed pathogenic bacterium. New strategies to develop novel antibiotics based on intrinsic bacterial toxin–antitoxin (TA) systems have been recently reported. Because TA systems are present only in bacteria and not in humans, these distinctive systems are attractive targets for developing antibiotics with new modes of action. S. aureus PemIK is a type II TA system, comprising the toxin protein PemK and the labile antitoxin protein PemI. Here, we determined the crystal structures of both PemK and the PemIK complex, in which PemK i

APA, Harvard, Vancouver, ISO, and other styles

39

amraei, Fatemeh, Negar narimisa, Behrooz sadeghi kalani, Rokhsareh mohammadzadeh, Vahid lohrasbi, and Faramarz masjedian jazi. "The expression of type II TA system genes following exposure to the sub-inhibitory concentration of gentamicin and acid stress in Brucella spp." Microbial Pathogenesis 144 (July 2020): 104194. http://dx.doi.org/10.1016/j.micpath.2020.104194.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Song, Cheng, Mingyue Zhang, Zongpu Jia, Weiping Peng, and Hairu Guo. "A lightweight batch anonymous authentication scheme for VANET based on pairing-free." Computer Science and Information Systems 15, no. 3 (2018): 549–67. http://dx.doi.org/10.2298/csis171222022s.

Full text

Abstract:

Aimed at improving the security and efficiency of anonymous authentication in vehicular ad hoc network (VANET), a certificateless batch anonymous authentication scheme without bilinear pairings is put forward. By coordinating Trust Authority (TA) and vehicles to generate the public/private key pairs and pseudonyms, the system security is freed from dependency on tamperproof devices. Through comprehensive analyses, this scheme is proved not only to be able to realize such security properties as authentication, anonymity, traceability, unforgeability, forward or backward security, etc., but also

APA, Harvard, Vancouver, ISO, and other styles

41

Asseck, Lisa Yasmin, Dietmar Gerald Mehlhorn, Jhon Rivera Monroy, et al. "Endoplasmic reticulum membrane receptors of the GET pathway are conserved throughout eukaryotes." Proceedings of the National Academy of Sciences 118, no. 1 (2020): e2017636118. http://dx.doi.org/10.1073/pnas.2017636118.

Full text

Abstract:

Type II tail-anchored (TA) membrane proteins are involved in diverse cellular processes, including protein translocation, vesicle trafficking, and apoptosis. They are characterized by a single C-terminal transmembrane domain that mediates posttranslational targeting and insertion into the endoplasmic reticulum (ER) via the Guided-Entry of TA proteins (GET) pathway. The GET system was originally described in mammals and yeast but was recently shown to be partially conserved in other eukaryotes, such as higher plants. A newly synthesized TA protein is shielded from the cytosol by a pretargeting

APA, Harvard, Vancouver, ISO, and other styles

42

Sofiev, M., R. Vankevich, M. Lotjonen, et al. "An operational system for the assimilation of the satellite information on wild-land fires for the needs of air quality modelling and forecasting." Atmospheric Chemistry and Physics 9, no. 18 (2009): 6833–47. http://dx.doi.org/10.5194/acp-9-6833-2009.

Full text

Abstract:

Abstract. This paper investigates a potential of two remotely sensed wild-land fire characteristics: 4-μm Brightness Temperature Anomaly (TA) and Fire Radiative Power (FRP) for the needs of operational chemical transport modelling and short-term forecasting of atmospheric composition and air quality. The treatments of the TA and FRP data are presented and a methodology for evaluating the emission fluxes of primary aerosols (PM2.5 and total PM) is described. The method does not include the complicated analysis of vegetation state, fuel load, burning efficiency and related factors, which are unc

APA, Harvard, Vancouver, ISO, and other styles

43

Wen, Wen, Banghui Liu, Lu Xue, Zhongliang Zhu, Liwen Niu, and Baolin Sun. "Autoregulation and Virulence Control by the Toxin-Antitoxin System SavRS inStaphylococcus aureus." Infection and Immunity 86, no. 5 (2018): e00032-18. http://dx.doi.org/10.1128/iai.00032-18.

Full text

Abstract:

ABSTRACTToxin-antitoxin (TA) systems play diverse physiological roles, such as plasmid maintenance, growth control, and persister cell formation, but their involvement in bacterial pathogenicity remains largely unknown. Here, we have identified a novel type II toxin-antitoxin system, SavRS, and revealed the molecular mechanisms of its autoregulation and virulence control inStaphylococcus aureus. Electrophoretic mobility shift assay and isothermal titration calorimetry data indicated that the antitoxin SavR acted as the primary repressor bound to its own promoter, while the toxin SavS formed a

APA, Harvard, Vancouver, ISO, and other styles

44

Ninkovic, Vladan, Srdjan Ninkovic, and Dragana Zivojinovic. "Cardiovascular autonomous dysfunction in diabetics: The influence of disease duration, glycoregulation degree and diabetes type." Srpski arhiv za celokupno lekarstvo 136, no. 9-10 (2008): 488–93. http://dx.doi.org/10.2298/sarh0810488n.

Full text

Abstract:

INTRODUCTION Cardiovascular autonomous neuropathy (CAN) in diabetes has not been still defined clinically and aetiopathogenetically. OBJECTIVE The aim of this study was to determine the influence of disease duration, glycoregulation degree and diabetes type on damage of the cardiovascular part of the autonomous nervous system in our group of patients. METHOD This study included diabetics, (100 patients) the same number of patients with diabetes type I and II as well as 20 healthy individuals in the control group. Classic Ewing's cardiovascular tests were used for CAN diagnosis: 1. the cardiova

APA, Harvard, Vancouver, ISO, and other styles

45

Tamman, Hedvig, Andres Ainelo, Mari Tagel, and Rita Hõrak. "Stability of the GraA Antitoxin Depends on Growth Phase, ATP Level, and Global Regulator MexT." Journal of Bacteriology 198, no. 5 (2015): 787–96. http://dx.doi.org/10.1128/jb.00684-15.

Full text

Abstract:

ABSTRACTBacterial type II toxin-antitoxin systems consist of a potentially poisonous toxin and an antitoxin that inactivates the toxic protein by binding to it. Most of the toxins regulate stress survival, but their activation depends on the stability of the antitoxin that has to be degraded in order for the toxin to be able to attack its cellular targets. The degradation of antitoxins is usually rapid and carried out by ATP-dependent protease Lon or Clp, which is activated under stress conditions. ThegraTAsystem ofPseudomonas putidaencodes the toxin GraT, which can affect the growth rate and

APA, Harvard, Vancouver, ISO, and other styles

46

Moreno-Córdoba, Inmaculada, Wai-Ting Chan, Concha Nieto, and Manuel Espinosa. "Interactions of the Streptococcus pneumoniae Toxin-Antitoxin RelBE Proteins with Their Target DNA." Microorganisms 9, no. 4 (2021): 851. http://dx.doi.org/10.3390/microorganisms9040851.

Full text

Abstract:

Type II bacterial toxin-antitoxin (TA) systems are found in most bacteria, archaea, and mobile genetic elements. TAs are usually found as a bi-cistronic operon composed of an unstable antitoxin and a stable toxin that targets crucial cellular functions like DNA supercoiling, cell-wall synthesis or mRNA translation. The type II RelBE system encoded by the pathogen Streptococcus pneumoniae is highly conserved among different strains and participates in biofilm formation and response to oxidative stress. Here, we have analyzed the participation of the RelB antitoxin and the RelB:RelE protein comp

APA, Harvard, Vancouver, ISO, and other styles

47

Ovčačíková, Hana, Marek Velička, Jozef Vlček, Michaela Topinková, Miroslava Klárová, and Jiří Burda. "Corrosive Effect of Wood Ash Produced by Biomass Combustion on Refractory Materials in a Binary Al–Si System." Materials 15, no. 16 (2022): 5796. http://dx.doi.org/10.3390/ma15165796.

Full text

Abstract:

In terms of its chemical composition, biomass is a very complex type of fuel. Its combustion leads to the formation of materials such as alkaline ash and gases, and there is evidence of the corrosive effect this process has on refractory linings, thus shortening the service life of the combustion unit. This frequently encountered process is known as “alkaline oxidative bursting”. Corrosion is very complex, and it has not been completely described yet. Alkaline corrosion is the most common cause of furnace-lining degradation in aggregates that burn biomass. This article deals with an experiment

APA, Harvard, Vancouver, ISO, and other styles

48

Bentivenga, C., N. E. Politi, A. Bragagni, et al. "AB0390 OVERACTIVATION OF THE RENIN ANGIOTENSIN SYSTEM AS A POSSIBLE CONTRIBUTOR TO THE INCREASED CARDIOVASCULAR RISK IN RHEUMATOID ARTHRITIS (RA); EVALUATION OF LEUKOCYTE EXPRESSION OF ANGIOTENSIN II RECEPTOR TYPE 1 AND TYPE 2 IN A POPULATION OF RA PATIENTS." Annals of the Rheumatic Diseases 82, Suppl 1 (2023): 1379.1–1379. http://dx.doi.org/10.1136/annrheumdis-2023-eular.5578.

Full text

Abstract:

Backgroundrheumatoid arthritis (RA) is a chronic systemic autoinflammatory disease of unknown aetiology characterized by joint inflammation and multiple comorbidities, with a prevalence of approximately 1% of the adult population. It is considered an independent cardiovascular (CV) risk factor, in fact it is associated with significantly increased CV morbidity and mortality. The renin angiotensin system (RAS) is a hormonal cascade with pleiotropic effects. Not only is it crucial in blood pressure regulation but it also plays an important role, among many other effects, in inflammation. High ci

APA, Harvard, Vancouver, ISO, and other styles

49

Kang, Sung-Min. "Mycobacterium tuberculosis Rv0229c Shows Ribonuclease Activity and Reveals Its Corresponding Role as Toxin VapC51." Antibiotics 12, no. 5 (2023): 840. http://dx.doi.org/10.3390/antibiotics12050840.

Full text

Abstract:

The VapBC system, which belongs to the type II toxin–antitoxin (TA) system, is the most abundant and widely studied system in Mycobacterium tuberculosis. The VapB antitoxin suppresses the activity of the VapC toxin through a stable protein–protein complex. However, under environmental stress, the balance between toxin and antitoxin is disrupted, leading to the release of free toxin and bacteriostatic state. This study introduces the Rv0229c, a putative VapC51 toxin, and aims to provide a better understanding of its discovered function. The structure of the Rv0229c shows a typical PIN-domain pr

APA, Harvard, Vancouver, ISO, and other styles

50

Zhang, Yan, Luyi Huang, Jie Li, et al. "Two-dimensional Ta₂NiSe₅/GaSe van der Waals heterojunction for ultrasensitive visible and near-infrared dual-band photodetector." Applied Physics Letters 120, no. 26 (2022): 261101. http://dx.doi.org/10.1063/5.0093745.

Full text

Abstract:

Dual-band photodetectors have attracted intensive attention because of the requirement of multiband information [such as visible (VIS) and near-infrared (NIR)] in multicolor imaging technology, in which additional information beyond human vision could assist object identification and navigations. The use of 2D materials can break the limitation of high cost of conventional epitaxial semiconductors and a complex cryogenic cooling system for multi-band detection, but there is still much room to improve the performance, especially in responsivity and signal noise ratio. Herein, we have fabricated

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!