Relevant bibliographies by topics / Hepatocyt

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  2. Dissertations / Theses
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Academic literature on the topic 'Hepatocyt'

Author: Grafiati
Published: 28 June 2021
Last updated: 25 April 2022

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Journal articles on the topic "Hepatocyt"

1

Mundiri, Nur Azmiati, Meta Maulida Damayanti, Maya Tejasari, Annisa Rahmah Furqaani, and R. A. Retno Ekowati. "Pengaruh Fraksi Air Buah Lemon terhadap Gambaran Morfologi Jaringan Hati Mencit Tua yang Diberi Pakan Tinggi Lemak." Jurnal Integrasi Kesehatan & Sains 1, no. 1 (January 31, 2019): 49–53. http://dx.doi.org/10.29313/jiks.v1i1.4321.

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Dislipidemia merupakan salah satu faktor risiko dari non alcoholic fatty liver disease (NAFLD). NAFLD mempunyai karakteristik steatosis hepatik, hepatocyte ballooning, inflamasi lobular, dan fibrosis. Kandungan flavonoid pada Citrus limon dipercaya dapat mencegah steatosis hepatik. Tujuan penelitian ini mengetahui pengaruh fraksi air buah lemon terhadap gambaran morfologi jaringan hati mencit tua yang diberi pakan tinggi lemak. Penelitian ini merupakan penelitian eksperimental dengan subjek penelitian adalah mencit (Mus musculus) jantan galur DDY tua yang dibagi menjadi empat kelompok secara acak, terdiri atas kelompok kontrol dan tiga kelompok perlakuan dengan konsentrasi fraksi air buah lemon 20,6; 41,2; 82,4 mg/20 gram BB mencit. Data jumlah hepatosit dengan droplet lemak dan hepatocyte ballooning dianalisis menggunakan uji ANOVA dan Uji Kruskal Willis. Terdapat perbedaan jumlah hepatosit dengan droplet lemak (p=0,063) dan hepatosit yang mengalami pembengkakan (p=0,109) antara kelompok kontrol dan kelompok perlakuan. Simpulan penelitian ini adalah fraksi air buah lemon dapat mencegah hepatocyte ballooning dan pembentukan droplet lemak pada hepatosit mencit tua yang diberikan pakan tinggi lemak. PROTECTIVE EFFECT OF WATER FRACTION OF LEMON ON HIGH-FAT DIET-INDUCED LIVER INJURY IN OLD MICEDyslipidemia is one of the risk factors of non alcoholic fatty liver disease (NAFLD). NAFLD is characterized by hepatic steatosis, hepatocyte ballooning, lobular inflammation, and fibrosis. Flavonoid in Citrus limon is believed to prevent hepatic steatosis. The aim of this study is to know the protective effect of lemon’s water fraction on high-fat diet-induced liver injury in old mice. This was an experimental study with old male mice (Mus musculus) DDY strain divided into four groups randomly, consisting of control group and three groups given with water fraction of lemon at concentration 20.6; 41.2; 82.4 mg/20 gram mice body weight. Total count of hepatocytes with fat droplets and hepatocytes ballooning were analyzed using ANOVA and Kruskal Willis tests. There are differences in the amount of hepatocytes with fat droplets (p=0.063) and hepatocytes ballooning (p=0.109) between the control group and the treatment group. The conclusion of this study is lemon’s water fraction can prevent the formation of hepatocyte ballooning and fat droplet in old mice’s hepatocyte fed by high-fat diet.
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Sibuea, Christine Verawaty, Enjelin Sasa Kristanti Hutabarat, and David M. T. Simangunsong. "Viabilitas Hepatosit pada Monokultur 3D Metode Hanging Drop dan Monokultur 2D." Nommensen Journal of Medicine 7, no. 2 (February 28, 2022): 36–38. http://dx.doi.org/10.36655/njm.v7i2.623.

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Background: Liver is a vital organ that has many functions including metabolism, detoxification of toxins and protein synthesis. The prevalence of liver disease is high and the treatment of liver disease continues to develop, so liver models are needed to study liver disease mechanisms and test drug toxicity. Many hepatocyte culture methods are being developed to construct an optimal liver model. The best culture method maintains high hepatocyte viability. Objective : This study aims to determine the viability of hepatocytes in 3D hanging drop method culture and in 2D culture. Methods: Hepatocytes were isolated from the liver of Sprague Dawley-Rats rats (n=2) and digested with Trypsin. Primary hepatocytes were cultured using the hanging drop method and conventional (2D) method. Hepatocyte viability was analyzed using the Trypan Blue Exclusion Test. Results: Hanging drop method had higher viability (81.18%) than the 2D culture method (69.22%) with p>0,05. Conclusion: The hanging drop method has better viability than the 2D culture method. Keywords: Hepatocyte, viability, 3D culture, 2D culture, hanging drop Latar belakang: Hati merupakan organ vital tubuh yang memiliki banyak fungsi diantaranya metabolisme, detoksifikasi racun dan sintesis protein. Prevalensi penyakit hati tinggi dan terapi penyakit hati terus berkembang, sehingga model hati sangat dibutuhkan untuk mempelahari mekanisme penyakit hati dan uji toksisitas obat. Banyak metode kultur hepatosit yang terus dikembangkan untuk menghasilkan suatu model hati yang optimal. Metode kultur yang terbaik adalah metode kultur dengan viabilitas hepatosit yang tinggi. Tujuan : Penelitian ini bertujuan untuk mengetahui viabilitas hepatosit pada kultur 3D metode hanging drop dan pada kultur 2D. Metode: Hepatosit diisolasi dari hati tikus Sprague Dawley-Rats (n=2) dan digesti dengan Tripsin. Hepatosit primer tikus dikultur dengan metode hanging drop (tetes gantung) dan metode konvensional (2D). Viabilitas hepatosit dianalisa dengan menggunakan Trypan Blue Exclusion Test. Hasil: Viabilitas hepatosit pada metode hanging drop memiliki viabilitas lebih tinggi (81,18%) dibandingan dengan metode kultur 2D (69,22%) dengan nilai p<5. Kesimpulan: Metode hanging drop memiliki viabilitas yang lebih baik dibandingkan metode kultur 2D. Kata Kunci: Hepatosit, viabilitas, kultur 3D, kultur 2D, hanging drop
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Skuratov, A. G., D. R. Petrenyov, and A. N. Kondrachuk. "EXPRESSION OF MARKER GENES BY HEPATOCYTE-LIKE CELLS differentiated from mesenchymal stem cells." Health and Ecology Issues, no. 3 (September 28, 2013): 105–10. http://dx.doi.org/10.51523/2708-6011.2013-10-3-22.

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Objective: to investigate the expression of marker genes by hepatocyte-like cells differentiated from mesenchymal stem cells (MSCs). Materials and methods. Wistar white rats, bone marrow MSCs, isolated hepatocytes of the rats were obtained by enzymatic perfusion of liver; differentiation of MSCs in hepatocyte direction; light microscopy; investigation of expression of genes by polymerase chain reaction (PCR). Results. The observed changes in the gene expression profile during the stages of differentiation indicate the presence of the cells differentiated into hepatocytic direction in MSCs culture. The expression of Carbox, Krt18, Krt19 Cyt1A1 genes depends on the composition of the medium and is not permanent and inducible in nature. It is important to go on searching for the molecular markers of MSCs differentiation in the hepatocytic direction. These results demonstrate the necessity to systematize the available data on the changes in the levels of gene expression during MSCs differentiation into hepatocytes to unify the conditions of assessment of the gene expression profiling.
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Sibuea, Christine Verawaty, Jeanne Adiwinata Pawitan, and Radiana Antarianto. "Pengaruh Penggantian Medium terhadap Viabilitas Hepatosit Kultur 3D Organoid Hati." Nommensen Journal of Medicine 7, no. 2 (February 28, 2022): 39–42. http://dx.doi.org/10.36655/njm.v7i2.625.

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Background : Liver organoids can be used as materials for Bioartificial Liver, to study the mechanism of liver disease and as drug test toxicity. Reconstruction of liver organoids requires optimal culture methods, culture medium and cellular components to construct liver organoids that resemble liver microstructure in vivo with optimal function. 3D culture method using hepatocytes and stem cells with PRP supplemented William's E can reconstruct liver organoids with liver function. Medium exchange is an usual method to maintain the required nutrients and to eliminate waste products, but it requires a sufficient supply of medium and supplementation. Method and the use of effective and efficient medium with optimal hepatocyte viability are urgently needed in the reconstruction of liver organoids. Objective : This study was aimed to compare the viability of primary hepatocytes in culture medium exchange liver organoids and monoculture and without culture medium exchange. Methods : Primary hepatocytes isolated from Sprague Dawley-Rats mice (250gr, n=3) were co-cultured with umbilical cord mesenchymal stem cells, cord blood CD34+ stem cells and LX2 in PRP-supplemented William's E for 14 days. The culture medium was exchanged at 48 hours, day 7 and day 14 and no culture medium exchanged in the control group. Hepatocyte viability was analyzed using the Trypan Blue Exclusion Test at 48 hours, day 7 and day 14. Results : Hepatocyte viability in culture medium exchange liver organoids was higher than without culture medium exchange, especially in monoculture, but there was no significant difference (p value> 0.05). Conclusion: Hepatocyte viability in culture medium exchange liver organoids was not significantly different from no culture medium exchange liver organoids. Culture medium exchange in monoculture supported hepatocyte viability up to day 14. Keywords: hepatocytes, liver organoids, viability, culture medium ABSTRAK Latar belakang : Organoid hati dapat digunakan sebagai bahan Bioartificial Liver, mempelajari mekanisme penyakit hati dan uji toksisitas obat. Rekonstruksi organoid hati membutuhkan metode kultur, medium kultur dan komponen seluler yang optimal untuk menghasilkan organoid hati yang menyerupai mikrostruktur hati in vivo dengan fungsi yang optimal. Metode kultur 3D menggunakan hepatosit dan sel punca mesenkimal dengan William’s E yang disuplementasi PRP dapat merekonstruksi organoid hati dengan fungsi hati. Pergantian medium merupakan metode yang sering dilakukan untuk mempertahankan nutrisi yang dibutuhkan dan untuk membuang sisa metabolit sel, tetapi membutuhkan persediaan medium dan suplementasi yang cukup banyak. Metode dan penggunaan medium yang efektif dan efisien dengan viabilitas hepatosit yang optimal sangat dibutuhkan dalam rekonstruksi organoid hati. Tujuan : Penelitian ini bertujuan untuk mengetahui perbandingan viabilitas hepatosit primer pada organoid hati dengan pergantian medium kultur dan tanpa pergantian medium kultur. Metode : Hepatosit primer yang diisolasi dari tikus Sprague Dawley-Rats (250gr, n=3) diko-kultur dengan sel punca mesenkimal asal tali pusat, sel punca CD34+ asal darah tali pusat dan LX2 dalam William’s E yang disuplementasi PRP selama 14 hari. Medium kultur diganti pada 48 jam, hari ke-7 dan hari ke-14 dan tidak dilakukan pergantian medium pada kelompok kontrol. Viabilitas hepatosit dianalisa dengan menggunakan Trypan Blue Exclusion Test pada 48 jam, hari ke-7 dan hari ke-14. Hasil : Viabilitas hepatosit pada organoid hati dengan pergantian medium kultur tampak lebih banyak dibandingkan tanpa pergantian medium kultur khususnya pada monokultur, tetapi tidak terdapat perbedaan yang signifikan (nilai p>0,05). Kesimpulan : Viabilitas hepatosit pada organoid hati dengan pergantian medium kultur tidak berbeda secara signifikan dengan organoid hati tanpa pergantian medium kultur. Pergantian medium kultur pada monokultur mendukung viabilitas hepatosit hingga hari ke-14. Kata Kunci : Hepatosit, organoid hati, viabilitas, medium kultur
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Gómez-Aristizábal, Alejandro, and John Edward Davies. "The effects of human umbilical cord perivascular cells on rat hepatocyte structure and functional polarity." Biochemistry and Cell Biology 91, no. 3 (June 2013): 140–47. http://dx.doi.org/10.1139/bcb-2012-0079.

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Hepatocyte culture is a useful tool for the study of their biology and the development of bioartificial livers. However, many challenges have to be overcome since hepatocytes rapidly lose their normal phenotype in vitro. We have recently demonstrated that human umbilical cord perivascular cells (HUCPVCs) are able to provide support to hepatocytes. In the present study we go further into exploring the effects that HUCPVCs have in the functional polarization, and both the internal and external organization, of hepatocytes. Also, we investigate HUCPVC–hepatocyte crosstalk by tracking both the effects of HUCPVCs on hepatocyte transcription factors and those of hepatocytes on the expression of hepatotrophic factors in HUCPVCs. Our results show that HUCPVCs maintain the functional polarity of hepatocytes ex vivo, as judged by the secretion of fluorescein into bile canaliculi, for at least 40 days. Transmission electron microscopy revealed that hepatocytes in coculture organize in an organoid-like structure embedded in extracellular matrix surrounded by HUCPVCs. In coculture, hepatocytes displayed a higher expression of C/EBPα, implicated in maintenance of the mature hepatocyte phenotype, and HUCPVCs upregulated hepatocyte growth factor and Jagged1 indicating that these genes may play important roles in HUCPVC–hepatocyte interactions.
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Naruse, K., Y. Sakai, I. Nagashima, G. X. Jiang, M. Suzuki, and T. Muto. "Comparisons of Porcine Hepatocyte Spheroids and Single Hepatocytes in the Non-Woven Fabric Bioartificial Liver Module." International Journal of Artificial Organs 19, no. 10 (October 1996): 605–9. http://dx.doi.org/10.1177/039139889601901008.

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We previously developed a new bioreactor of the bioartificial liver composed of non-woven fabric. We have also experimented with hepatocyte spheroids, with the aim of improving the efficiency of this NWF bioreactor. In this study, we compared the efficiencies of NWF bioreactors employing hepatocyte spheroids versus single hepatocytes. Hepatocytes were isolated from a whole pig liver by Seglen's method. 1.0 × 1010 single hepatocytes were immobilized in the NWF bioreactor. Another 1.0 × 1010 hepatocytes were allowed to form spheroids by 24 hr suspension culture in a 4-L culture vessel, before being immobilized in the bioreactor. Hepatocyte spheroids were found to be functionally superior, on a per-cell basis, to single hepatocytes in the NWF bioreactor. However, the NWF bioreactor employing hepatocyte spheroids exhibited lower efficiency than that employing single hepatocytes, because the total number of the hepatocytes had decreased during the 24 hr suspension culture.
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Mason, William S., Chunxiao Xu, Huey Chi Low, Jeffry Saputelli, Carol E. Aldrich, Catherine Scougall, Arend Grosse, Richard Colonno, Sam Litwin, and Allison R. Jilbert. "The Amount of Hepatocyte Turnover That Occurred during Resolution of Transient Hepadnavirus Infections Was Lower When Virus Replication Was Inhibited with Entecavir." Journal of Virology 83, no. 4 (December 10, 2008): 1778–89. http://dx.doi.org/10.1128/jvi.01587-08.

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ABSTRACT Transient hepadnavirus infections can involve spread of virus to the entire hepatocyte population. In this situation hepatocytes present following recovery are derived from infected hepatocytes. During virus clearance antiviral cytokines are thought to block virus replication and formation of new covalently closed circular DNA (cccDNA), the viral transcriptional template. It remains unclear if existing cccDNA is eliminated noncytolytically or if hepatocyte death and proliferation, to compensate for killing of some of the infected hepatocytes, are needed to remove cccDNA from surviving infected hepatocytes. Interpreting the relationship between hepatocyte death and cccDNA elimination requires knowing both the amount of hepatocyte turnover and whether cccDNA synthesis is effectively blocked during the period of immune destruction of infected hepatocytes. We have addressed these questions by asking if treatment of woodchucks with the nucleoside analog inhibitor of viral DNA synthesis entecavir (ETV) reduced hepatocyte turnover during clearance of transient woodchuck hepatitis virus (WHV) infections. To estimate hepatocyte turnover, complexity analysis was carried out on virus-cell DNA junctions created by integration of WHV and present following recovery in the livers of WHV-infected control or ETV-treated woodchucks. We estimated that, on average, 2.2 to 4.8 times less hepatocyte turnover occurred during immune clearance in the ETV-treated woodchucks. Computer modeling of the complexity data suggests that mechanisms in addition to hepatocyte death were responsible for elimination of cccDNA during recovery from transient infections.
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Gupta, Sanjeev, Pankaj Rajvanshi, Emma Aragona, Chang-Don Lee, Purnachandra R. Yerneni, and Robert D. Burk. "Transplanted hepatocytes proliferate differently after CCl4 treatment and hepatocyte growth factor infusion." American Journal of Physiology-Gastrointestinal and Liver Physiology 276, no. 3 (March 1, 1999): G629—G638. http://dx.doi.org/10.1152/ajpgi.1999.276.3.g629.

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To understand regulation of transplanted hepatocyte proliferation in the normal liver, we used genetically marked rat or mouse cells. Hosts were subjected to liver injury by carbon tetrachloride (CCl4), to liver regeneration by a two-thirds partial hepatectomy, and to hepatocellular DNA synthesis by infusion of hepatocyte growth factor for comparative analysis. Transplanted hepatocytes were documented to integrate in periportal areas of the liver. In response to CCl4 treatments after cell transplantation, the transplanted hepatocyte mass increased incrementally, with the kinetics and magnitude of DNA synthesis being similar to those of host hepatocytes. In contrast, when cells were transplanted 24 h after CCl4 administration, transplanted hepatocytes appeared to be injured and most cells were rapidly cleared. When hepatocyte growth factor was infused into the portal circulation either subsequent to or before cell transplantation and engraftment, transplanted cell mass did not increase, although DNA synthesis rates increased in cultured primary hepatocytes as well as in intact mouse and rat livers. These data suggested that procedures causing selective ablation of host hepatocytes will be most effective in inducing transplanted cell proliferation in the normal liver. The number of transplanted hepatocytes was not increased in the liver by hepatocyte growth factor administration. Repopulation of the liver with genetically marked hepatocytes can provide effective reporters for studying liver growth control in the intact animal.
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Pastor, Catherine M., and Valérie Vilgrain. "Steatosis Alters the Activity of Hepatocyte Membrane Transporters in Obese Rats." Cells 10, no. 10 (October 13, 2021): 2733. http://dx.doi.org/10.3390/cells10102733.

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Fat accumulation (steatosis) in ballooned hepatocytes alters the expression of membrane transporters in Zucker fatty (fa/fa) rats. The aim of the study was to quantify the functions of these transporters and their impact on hepatocyte concentrations using a clinical hepatobiliary contrast agent (Gadobenate dimeglumine, BOPTA) for liver imaging. In isolated and perfused rat livers, we quantified BOPTA accumulation and decay profiles in fa/+ (normal) and fa/fa hepatocytes by placing a gamma counter over livers. Profiles of BOPTA accumulation and decay in hepatocytes were analysed with nonlinear regressions to characterise BOPTA influx and efflux across hepatocyte transporters. At the end of the accumulation period, BOPTA hepatocyte concentrations and influx clearances were not significantly different in fa/+ and fa/fa livers. In contrast, bile clearance was significantly lower in fatty hepatocytes while efflux clearance back to sinusoids compensated the low efflux into canaliculi. The time when BOPTA cellular efflux impacts the accumulation profile of hepatocyte concentrations was slightly delayed (2 min) by steatosis, anticipating a delayed emptying of hepatocytes. The experimental model is useful for quantifying the functions of hepatocyte transporters in liver diseases.
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GHIURCO, Ioan Florin, Aurel DAMIAN, Vasile Florin RUS, Cristian MARTONOS, Maria Cătălina MATEI, Victoria BUZA, Laura Cristina ȘTEFĂNUȚ, et al. "Mitochondria Load Degree in Hepatocytes of the Classical Hepatic Lobules in Chinchilla (Chinchilla lanigera)." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine 78, no. 1 (May 4, 2021): 76. http://dx.doi.org/10.15835/buasvmcn-m:2021.0004.

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Hepatocytes represent the majority of the liver cell population and are arranged in the form of cords placed in intimate contact with the sinusoidal capillaries. The functional complexity corroborated with the intensity of the activity of hepatocytes requires large amounts of energy. The organelles involved in the production of chemical energy used in the activity of hepatocytes are the mitochondria. The purpose of this study was to verify the mitochondrial load of hepatocytes in all areas of the classical hepatic lobules, in order to indirectly assess the intensity of hepatocyte activity in each area. Materials and Methods Five fresh corpses of chinchilla (Chinchilla lanigera) from an independent breeder from Bistrița-Năsăud county were used. Liver fragments were harvested and fixated in Kolster’s solution for 24 hours, stained with Heidenhain ferric hematoxylin, and assessed using Olympus BX41 microscope. Fixation with Kolster's solution and the staining with Heidenhain's iron hematoxylin clearly shows the hepatocytic mitochondria in shades from gray to black. The liver lobules displayed an uneven distribution of mitochondria depending on the area. In zone 1 of the classical hepatic lobule, the degree of loading of hepatocytes with mitochondria is larger than in zone 2 and much larger than in zone 3. Morphological features of the hepatocytes, including the number and distribution of mitochondria in the hepatic lobules, should improve the understanding of the physiology and pathology of the liver.
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Dissertations / Theses on the topic "Hepatocyt"

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Ayubi, Nawid. "mRNA-Expression vom connective tissue growth factor (CTGF) und hepatocyt growth factor (HGF) nach laserinduzierter Thermotherapie (LITT) und chirurgischer Resektion experimenteller Lebermetastasen." [S.l.] : [s.n.], 2004. http://www.diss.fu-berlin.de/2004/183/index.html.

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Schuster, Susanne, and Antje Garten. "Resveratrol Differentially Regulates NAMPT and SIRT1 in Hepatocarcinoma Cells and Primary Human Hepatocytes." Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-142581.

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Resveratrol is reported to possess chemotherapeutic properties in several cancers. In this study, we wanted to investigate the molecular mechanisms of resveratrol-induced cell cycle arrest and apoptosis as well as the impact of resveratrol on NAMPT and SIRT1 protein function and asked whether there are differences in hepatocarcinoma cells (HepG2, Hep3B cells) and non-cancerous primary human hepatocytes. We found a lower basal NAMPT mRNA and protein expression in hepatocarcinoma cells compared to primary hepatocytes. In contrast, SIRT1 was significantly higher expressed in hepatocarcinoma cells than in primary hepatocytes. Resveratrol induced cell cycle arrest in the S- and G2/M- phase and apoptosis was mediated by activation of p53 and caspase-3 in HepG2 cells. In contrast to primary hepatocytes, resveratrol treated HepG2 cells showed a reduction of NAMPT enzymatic activity and increased p53 acetylation (K382). Resveratrol induced NAMPT release from HepG2 cells which was associated with increased NAMPT mRNA expression. This effect was absent in primary hepatocytes where resveratrol was shown to function as NAMPT and SIRT1 activator. SIRT1 inhibition by EX527 resembled resveratrol effects on HepG2 cells. Furthermore, a SIRT1 overexpression significantly decreased both p53 hyperacetylation and resveratrol-induced NAMPT release as well as S-phase arrest in HepG2 cells. We could show that NAMPT and SIRT1 are differentially regulated by resveratrol in hepatocarcinoma cells and primary hepatocytes and that resveratrol did not act as a SIRT1 activator in hepatocarcinoma cells.
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Matz-Soja, Madlen, and Rolf Gebhardt. "Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels." Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-142560.

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Background Hedgehog signaling plays an important role in embryonic development, organogenesis and cancer. In the adult liver, Hedgehog signaling in non-parenchymal cells has been found to play a role in certain disease states such as fibrosis and cirrhosis. However, whether the Hedgehog pathway is active in mature healthy hepatocytes and is of significance to liver function are controversial. Findings Two types of mice with distinct conditional hepatic deletion of the Smoothened gene, an essential co-receptor protein of the Hedgehog pathway, were generated for investigating the role of Hedgehog signaling in mature hepatocytes. The knockout animals (KO) were inconspicuous and healthy with no changes in serum transaminases, but showed a slower weight gain. The liver was smaller, but presented a normal architecture and cellular composition. By quantitative RT-PCR the downregulation of the expression of Indian hedgehog (Ihh) and the Gli3 transcription factor could be demonstrated in healthy mature hepatocytes from these mice, whereas Patched1 was upregulated. Strong alterations in gene expression were also observed for the IGF axis. While expression of Igf1 was downregulated, that of Igfbp1 was upregulated in the livers of both genders. Corresponding changes in the serum levels of both proteins could be detected by ELISA. By activating and inhibiting the transcriptional output of Hedgehog signaling in cultured hepatocytes through siRNAs against Ptch1 and Gli3, respectively, in combination with a ChIP assay evidence was collected indicating that Igf1 expression is directly dependent on the activator function of Gli3. In contrast, the mRNA level of Igfbp1 appears to be controlled through the repressor function of Gli3, while that of Igfbp2 and Igfbp3 did not change. Interestingly, body weight of the transgenic mice correlated well with IGF-I levels in both genders and also with IGFBP-1 levels in females, whereas it did not correlate with serum growth hormone levels. Conclusions Our results demonstrate for the first time that Hedgehog signaling is active in healthy mature mouse hepatocytes and that it has considerable importance for IGF-I homeostasis in the circulation. These findings may have various implications for mouse physiology including the regulation of body weight and size, glucose homeostasis and reproductive capacity.
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Hannoun, Zara. "Role of SUMO modification in hepatocyte differentiation." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/5917.

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Primary human hepatocytes are a scarce resource with variable function, which diminishes with time in culture. As a consequence their use in tissue modelling and therapy is restricted. Human embryonic stem cells (hESCs) could provide a stable source of human tissue due to their properties of self-renewal and their ability to give rise to all three germ layers. hESCs have the potential to provide an unlimited supply of hepatic endoderm (HE) which could offer efficient tools for drug discovery, disease modelling and therapeutic applications. In order to create a suitable environment to enhance HE formation, hESC culture needed to be standardised. As such, a media trail was carried out to define serum free media capable of maintaining hESC in a pluripotent undifferentiated state. We also ensured hESC cultured in the various media could be directly differentiated to HE in a reproducible and efficient manner. The project then focused on the effect of post-translational modifications (PTMs), specifically SUMOylation, in hepatocyte differentiation and its subsequent manipulation to enhance HE viability. SUMOylation is a PTM known to modify a large number of proteins that play a role in various cellular processes including: cell cycle regulation, gene transcription, differentiation and cellular localisation. We hypothesised that SUMO modification may not only regulate hESC self renewal, but also maybe required for efficient hESC differentiation. We therefore interrogated the role of SUMOylation in hESC differentiation to hepatic endoderm (HE). hESC were differentiated and the cellular lysates were analysed by Western blotting for key proteins which modulate the conjugation and de conjugation of SUMO. We demonstrate that peak levels of SUMOylation were detectable in hESC populations and during cellular differentiation to definitive endoderm (DE), day 5. Following commitment to DE we observed a decrease in the level of SUMO modified proteins during cellular specialisation to a hepatic fate, corresponding with an increase in SENP 1, a SUMO deconjugation enzyme. We also detected reduced levels of hepatocyte nuclear factor 4 α (HNF4α), a critical regulator of hepatic status and metabolic function, as SUMOylation decreased. As a result, we investigated if HNF4α was SUMOylated and if this process was involved in modulating HNF4α’s critical role in HE. HNF4α is an important transcription factor involved in liver organogenesis during development and is a key regulator for efficient adult liver metabolic functions. We observed a decreasing pattern of HNF4α expression at day 17 of our differentiation protocol in conjunction with a decrease in SUMO modified proteins. In order to further investigate and validate a role of SUMOylation on HNF4α stability Immunoprecipitation (IP) was employed. HNF4α protein was pulled down and probed for SUMO 2. Results show an increase in the levels of SUMO2 modification as the levels of HNF4α decrease. Through deletion and mutation analysis we demonstrated that SUMO modification of HNF4α was restricted to the C-terminus on lysine 365. Protein degradation via the proteasome was responsible for the decrease in HNF4α, demonstrated by the use of a proteasome 26S inhibitor MG132. Additionally, a group at the University of Dundee has shown that polySUMOylation of promyelocytic leukaemia protein (PML) leads to its subsequent ubiquitination via RNF4, an ubiquitin E3 ligase, driving its degradation. Using an in vitro ubiquitination assay, we show that polySUMOylated HNF4α is preferentially ubiquitinated in the presence of RNF4. Overall polySUMOylation of HNF4α may reduce its stability by driving its degradation, hence regulating protein activity. In conclusion, polySUMOylation of HNF4α is associated with its stability. HNF4α is subsequently important for HE differentiation both driving the formation of the hepatocytes and in maintaining a mature phenotype, in agreement with a number of different laboratories. Creating the ideal environment for sustaining mature functional hepatocytes, primary and those derived from hESCs and iPSCs, is essential for further use in applications such as drug screening, disease modelling and extracorporeal devices.
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Constante, Pereira Marco. "Development of synthetic biology devices for iron metabolism research." Doctoral thesis, Universitat Pompeu Fabra, 2011. http://hdl.handle.net/10803/53579.

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Synthetic biology is a fairly recent field that aims to engineer novel functions in biological systems. In a broad sense synthetic biology encompasses the development of tools that makes the engineering of biology easier. In this thesis I develop a collection of standard DNA parts (Biobricks) that consists of a tool to build custom eukaryotic plasmids. This is not just intended for biology researchers in the field of synthetic biology, but also for more general use. Besides the development of molecular biology tools that facilitate the engineering of biology, synthetic biology researchers have implemented devices that are electronics-like in behavior and have demonstrated the potential of the field for the production of biofuels, pharmaceutics and biosensors. Here I present a sensor of iron regulatory protein activity, based on Biobricks. To demonstrate its use I apply it to the study of a novel reconstituted two cell-type co-culture (BNL CL.2 and RAW 264.7), surrogate for hepatocyte-macrophage communication
La biología sintética es un campo recientemente desarrollado con el objectivo de implementar nuevas funciones en sistemas biológicos. De forma global, la biología sintética incluye el desarrollo de herramientas para facilitar la ingeniería de sistemas biológicos. En diversas publicaciones, investigadores en el campo de la biología sintética han implementado dispositivos que funcionan de forma similar a circuitos electrónicos y han demonstrado el potencial del campo para la producción de biocarburantes, farmaceuticos y biosensores. Para la presente tesis he creado una colección de plasmidos estandarizados (Biobricks) que pueden ser de interés para biólogos fuera del campo da la biología sintética. Además, utilizando estos Biobricks, he creado un sensor de la actividad de las proteínas reguladas por el hierro. Para demonstrar su aplicación, he utilizado el sensor para estudiar un nuevo sistema de co-cultura de dos tipos celulares (BNL CL.2 y RAW 264.7), substituto para la comunicación entre hepatocitos y macrófagos
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Vroemen, Joseph Pieter Anna Maria. "Hepatocyte transplantation." Maastricht : Maastricht : Rijksuniversiteit Limburg ; University Library, Maastricht University [Host], 1987. http://arno.unimaas.nl/show.cgi?fid=5364.

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Takeda, Yoshihisa. "Morphologic alteration of hepatocytes and sinusoidal endothelial cells in rat fatty liver during cold preservation and the protective effect of hepatocyte growth factor." Kyoto University, 1999. http://hdl.handle.net/2433/181710.

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Beckers, Simone [Verfasser], and Elmar [Akademischer Betreuer] Heinzle. "High throughput toxicity, physiological and metabolic studies for the characterization of hepatocytes and human embryonic stem cell derived hepatocyte-like cells / Simone Beckers. Betreuer: Elmar Heinzle." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2011. http://d-nb.info/1051095166/34.

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Kolatsi, Maria. "Hepatocyte growth factor and renal morphogenesis." Thesis, University College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243452.

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Dunlay, Samantha, and Jonathan M. Peterson. "CTRP3 Prevents ETOH- Induced Hepatocyte Apoptosis." Digital Commons @ East Tennessee State University, 2016. https://dc.etsu.edu/etsu-works/127.

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Books on the topic "Hepatocyt"

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Dhawan, Anil, and Robin D. Hughes, eds. Hepatocyte Transplantation. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-201-4.

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Stock, Peggy, and Bruno Christ, eds. Hepatocyte Transplantation. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6506-9.

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Maurel, Patrick, ed. Hepatocytes. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-688-7.

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Berry, Michael N., and Anthony M. Edwards, eds. The Hepatocyte Review. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8.

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Hepatocyte transplantation: Methods and protocols. New York, NY: Humana, 2009.

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David, Heinz. The hepatocyte: Development, differentation, and ageing. Jena: VEB Gustav Fischer, 1985.

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Rogiers, Vera, and Mathieu Vinken. Protocols in in vitro hepatocyte research. New York: Humana Press, 2015.

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Boros, Peter. Hepatocyte growth factor: The basic principles. Austin: R.G. Landes, 1999.

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Vinken, Mathieu, and Vera Rogiers, eds. Protocols in In Vitro Hepatocyte Research. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2074-7.

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Hepatocytes: Methods and protocols. New York: Humana, 2010.

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Book chapters on the topic "Hepatocyt"

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Berry, Michael N. "Isolated hepatocytes: forty years on." In The Hepatocyte Review, 1–9. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8_1.

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Guillouzo, André, Claire Guyomard, Alain Fautrel, and Christophe Chesné. "Storage of isolated hepatocytes." In The Hepatocyte Review, 125–45. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8_10.

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Meijer, Alfred J. "Hepatocyte swelling: techniques and effects on metabolism." In The Hepatocyte Review, 147–67. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8_11.

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Declercq, Peter E., and Myriam I. Baes. "Permeabilisation of hepatocytes with α-toxin." In The Hepatocyte Review, 169–80. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8_12.

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Farghali, Hassan. "Perifused immobilized hepatocytes for metabolic studies." In The Hepatocyte Review, 181–93. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8_13.

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Mitaka, Toshihiro, and Fumihiko Sato. "Small hepatocytes in primary cultures." In The Hepatocyte Review, 195–208. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8_14.

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Christoffersen, Thoralf, G. Hege Thoresen, Olav F. Dajani, Øyvind Melien, Tormod Guren, Magne Refsnes, and Dagny Sandnes. "Mechanisms of hepatocyte growth regulation by hormones and growth factors." In The Hepatocyte Review, 209–46. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8_15.

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Gómez-Lechón, María José, Isabel Guillén, M. J. Teresa Donato, Xavier Ponsoda, Ramiro Jover, and José V. Castell. "Proliferative response and metabolic effects of growth factors in human hepatocytes." In The Hepatocyte Review, 247–61. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8_16.

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Ilyin, Gennady, Claude Rescan, Mickaël Rialland, Pascal Loyer, Georges Baffet, and Christiane Guguen-Guillouzo. "Growth control and cell cycle progression in cultured hepatocytes." In The Hepatocyte Review, 263–80. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8_17.

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Maeda, Sumio. "Mechanisms of active cell death in isolated hepatocytes." In The Hepatocyte Review, 281–300. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-3345-8_18.

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Conference papers on the topic "Hepatocyt"

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Ingerslev, J., B. Sloth Christiansen, A. Bukh, S. Stenbjerg, T. Munck Jørgensen, and C. Munck Petersen. "HUMAN HEPATOCYTES CONTAIN HIGH MOLECULAR WEIGHT POLYPEPTIDES OF FACTOR VIII." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644324.

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Human hepatocytes were isolated by the two-step collagenase technique applied on distal left liver lobe. Homogenous and large cells were isolated revealing hepatocyte characteristics by light-microscopy. Hepatocytes were washed repeatedly in albumine buffer (5%), resuspended in the same buffer and sonicated using a cell density of 0.75 × 106 cells/ml. In some cases cells were separated from non-viable cells by flotation on a linear Percoll gradient. Supernate material after sonication was subjected to ELISA for VIII:Ag using human antibodies and vWf:Ag by polyclonal antibodies. Freshly isolated cells contained at least 0.25 IU/ 0.75 × 106 hepatocytes, whereas the vWf:Ag was below 0.01 IU/ 0.75 × 106 cells. The material obtained from sonication was further studied using fast protein liquid chromatography by Mono-Q HR 5/5 revealing a single peak of VIII: Ag eluting in the same position as the high molecular weight polypeptides of VIII :Ag of high purity FVIII derived from the plasma source. Isolated hepatocytes also were cultivated at 37°C in medium RPMI 1640 supplemented with Ultroser G (4%), glutamine and antibiotics. Cells secreted increasing quantities of albumin, fitrinogpn and protease-inhibitors. The supernatants also contained VIII: Ag in quantities ranging from 0.04 - 0.17 IU/ml after 24 hours, but no further secretion was observed. No vWf: Ag could be detected. Cells harvested and sonicated after 30 hours of culture only contained 0.04 IU/ 0.75 × 106 cells. Our results shows, that VIII :Ag is present in freshly isolated human hepatocytes and that only traces of vWf:Ag is found. A hepatocyte site of production of VIII is speculated. These very preliminary findings do not permit conclusions concerning active synthesis of VIII in hepatocytes. Further studies are underway.
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Qureshi, G. D., M. Sun, C. Gervin, and H. Evans. "RAT HEPAT0CYTES IN CULTURE INACTIVATE FACTOR Xa." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643294.

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Plasma contains zymogens of clotting factors, which under various stimuli are activated to serine proteases. Whereas much knowledge has been gained about the activation of clotting factors, relatively little is known about inactivation of these proteases. Antithrombin III has been shown to inactivate some activated clotting factors in plasma. Studies in intact animals have suggested that activated clotting factors are mainly inactivated in the liver. To investigate more fully the role of liver in inactivating the activated factors, we studied the stability of activated factor X(Xa) in hepatocyte cultures. Monolayer cultures on non-proliferating rat hepatocytes were prepared according to the method of Bissell et al. The culture medium was chemically defined and was free from serum or serum products. After the 24 h stabilization period, 0.5 units/ml of 100% activated bovine factor Xa was co-cultured with hepatocytes for 8 h. Samples were collected at 0, ½, 1 2, 4 and 8 h and tested for Xa activity using chromogenic substrate S-2222. At the end of 8 h only 41.07% of the initial Xa activity remained. Xa inactivation was not affected by a commercially prepared unfractionated heparin (1 unit/ml) and estradiol at 12.5, 25, 125 nM, a potentiator and inhibitor of antithrombin III, respectively. Inactivation of Xa in hepatocyte cultures was inhibited by the addition of cycloheximide (10-4M). Our data suggests that factor Xa is inactivated in hepatocyte cultures by one or more hepatic derived factors which do not meet the functional characteristics of antithrombin III.
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Shi, Gengbei, and Robin N. Coger. "Enhanced Oxygen Delivery to Liver Tissue Equivalent by Perfluorocarbon." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19190.

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Bioartificial Liver Devices (BALs) have the potential to serve as a bridge strategy for patients awaiting liver transplants, and also show promise as drug testing platforms for the pharmaceutical industry [1]. Yet the limitations of O2 transport through the 3D tissue structures of current designs continue to present engineering challenges. In previous work our group successfully improved O2 availability for hepatocytes by introducing micropathways within the BAL’s cellular space [3–5]. The current study investigates the benefits of increasing O2 availability of the flow medium via perfluorocarbons (PFCs). PFCs were chosen since they have demonstrated effectiveness in increasing the overall oxygen solubility of their carrier liquids, e.g., artificial blood [2]. The study seeks to clarify the effects of PFCs on hepatocyte viability and functional performance under various culture conditions.
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Kumar, B. Karthik, James P. Cassell, and Robin N. Coger. "Computational Model to Predict Oxygen Availability for Cells Cultured Under Flow Conditions." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19334.

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Tissue bioreactors are flow systems able to support cultured cells over time. Consequently the functionality of these in vivo systems require the cells to be provided with the required substrate, nutrient, and O2. When a bioreactor is used for liver support, the latter requirement is particularly critical since hepatocyte functionalities are highly dependent on O2 [1]. So for bioreactor designers, computational models would serve as a powerful tool for predicting O2 transport dynamics. In the current study, such a model is developed to predict the level of O2 available to hepatocytes dispersed within a porous collagen substrate, for varying flow conditions. This is carried out by intially verifying the model’s accuracy through experiments conducted using a customized flow device and then extending the model to clarify the sensitivity of O2 transport through the cellular space with respect to the flow conditions, the cells’ O2 consumption rates and dissolved O2 levels in the media.
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Hsu, MJ, M. Hempel, H. Kühne, and B. Christ. "Physical contact between mesenchymal stromal cells and hepatocytes via tunneling nanotubes favor the utilization of hepatocyte lipids." In 35. Jahrestagung der Deutschen Arbeitsgemeinschaft zum Studium der Leber. Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0038-1677163.

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Fowlkes, P. M., P. K. Lund, M. Blake, and J. Snouwaert. "THE REGULATION OF FIBRINOGEN PRODUCTION INVOLVES AT LEAST ONE OTHER HEPATOCYTE GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644317.

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It is currently thought that glucocorticosteriods have a direct effect on the transcription of the alpha, beta and gamma fibrinogen genes. However, our studies indicate that while corticosteriods play a role in fibrinogen production, this role is not due to transcriptional activation via glucocorticosteriod receptors. In initial experiments, we compared the levels of fibrinogen mRNA in hepatocytes isolated from hypophysectomized rats to those from control animals. The levels of mRNA in hypophysectomized rats, which produce little ACTH or corticosteriods, were significantly higher than the levels in control animals. Albumin mRNA levels were unaffected by hypophysectomy. These results are in opposition to those which we had anticipated. Based on previously published data, we had thought that physiologic deprivation of corticosteriods would lead to decreased levels of fibrinogen. We propose that these results are related to the negative feedback that corticosteroids have on Hepatocyte Stimulating Factor (HSF) production through a tightly controlled feedback circuit. To investigate the role of corticosteriods in fibrinogen gene regulation, we have conducted experiments with primary hepatocytes in culture and rat FAZA cells (continuous hepatoma cell line). There is a 4 to 5 fold increase in fibrinogen production when these cells are treated with HSF but no change when these cells are treated with dexamethasone alone. However, there is a marked additional increase in the production of fibrinogen with the combination of dexamethasone and HSF. Data gathered through kinetic analysis of this synergistic interaction suggest that the maximum response to HSF requires another gene product whose production is responsive to dexamethasone. Detailed analysis of the rate of transcription of thegamma fibrinogen gene, its processing and mRNA turnover suggests a specific role for this gene product in regulating fibrinogen synthesis. Characterization of this gene product will lead to greater understanding of the regulation of the Acute Phase Reactants.
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Eggert, S., F. A. Alexander, and J. Wiest. "Enabling 3D hepatocyte spheroids for microphysiometry." In 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2017. http://dx.doi.org/10.1109/embc.2017.8037148.

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Shirakashi, Ryo, Tomomi Yoshida, Christophe Provin, Kiyoshi Takano, Yasuyuki Sakai, and Teruo Fujii. "Steady Measurement of Glucose Metabolism of Hepatocyte." In ASME/JSME 2007 Thermal Engineering Heat Transfer Summer Conference collocated with the ASME 2007 InterPACK Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/ht2007-32750.

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Production of hybrid artificial organs for implantation is one of the main topics of tissue engineering. A large organ consisting of soft tissues requires a high cell density, c.a. 108 cells/mL, to satisfy the same physiological metabolic rate per organ-volume as an organ in vivo. Therefore, the supply of oxygen and nutrition to all the cells composing the soft tissue is always critical problem for the in vitro artificial organ production. Energy metabolic rates, such as oxygen and glucose metabolism rate, of single cell at various temperatures are the basic data for designing the oxygen and nutrition transport in an artificial organ. It is reported that several conditions including pH, temperature, oxygen or glucose concentration have effects on energy metabolism, although these interactions are not clearly quantitatively measured mainly because of the problems of measuring systems. In this study, convenient method to measure glucose consumption rate of hepatocyte (HepG2 cell line) at different temperature and glucose concentration is proposed. A device for the measurement was developed which consists of a small closed chamber with an inlet and an outlet of culture medium at the both ends of the chamber. On the one side of the walls in the chamber, confluent HepG2 on a coverslip was installed. Culture medium supplemented with various concentration of glucose was supplied to the open flow chamber in a constant flow rate. The whole chamber was in a thermostatic bath to keep the temperature in the chamber constant. Glucose consumption rate can be calculated by measuring the difference between glucose concentration of inlet culture medium and outlet culture medium, the flow rate and the number of cells in the chamber. Enzymatic analysis using D-Glucose-HK allows quantification of the sample glucose concentration. The advantages of the proposed method include; 1) small number of cells is required for the measurement, c. a. 105cells, 2) the flow pattern and the glucose supply are in steady state. Especially the latter advantage made it possible to evaluate the effects of different conditions on the glucose consumption rate. Since the most of the metabolic rate were measured under unsteady state, conditions, such as pH, oxygen concentration and glucose concentration, were changed sometime drastically during the measurement. The results provided the several parameters of Michaelis-Menten kinetics at various temperatures.
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Li, Lulu, Rene Schloss, Noshir Langrana, and Martin Yarmush. "Effects of Encapsulation Microenvironment on Embryonic Stem Cell Differentiation." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192587.

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Pluripotent embryonic stem cells represent a promising renewable cell source to generate a variety of differentiated cell types. Although many investigators have described techniques to effectively differentiate stem cells into different mature cell lineages, their practicality is limited by the absence of large scale processing consideration and low yields of differentiated cells. Previously we have established a murine embryonic stem cell alginate-poly-l-lysine microencapsulation differentiation system. The three-dimensional alginate microenvironment maintains cell viability, is conducive to ES cell differentiation to hepatocyte lineage cells, and maintains differentiated cellular function. In the present work, we demonstrate that hepatocyte differentiation is mediated by cell-cell aggregation in the encapsulation microenvironment. Both cell aggregation and hepatocyte functions, such as urea and albumin secretion, as well as increased expression of cytokaratin 18 and cyp4507a, occur concomitantly with surface E-cadherin expression. Furthermore, by incorporating soluble inducers, such as retinoic acid, into the permeable microcapsule system, we demonstrate decreased cell aggregation and enhanced neuronal lineage differentiation with the expression of various neuronal specific markers, including neurofilament, A2B5, O1 and GFAP. Therefore, as a result of capsule parameter and microenvironment manipulation, we are capable of targeting cellular differentiation to both endodermal and ectodermal cell lineages.
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Ke, Ling-Yi, Yu-Shih Chen, Jing Liu, and Cheng-Hsien Liu. "Cryogenic frozen device for hepatocyte culture and responses." In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2012. http://dx.doi.org/10.1109/nems.2012.6196745.

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Reports on the topic "Hepatocyt"

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Knudsen, Beatrice S. Hepatocyte Growth Factor and Interleukin-6 in Prostate Cancer Bone Metastasis. Fort Belvoir, VA: Defense Technical Information Center, March 2005. http://dx.doi.org/10.21236/ada435856.

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Knudesen, Beatrice S. Hepatocyte Growth Factor and Interleukin-6 in Prostate Cancer Bone Metastasis. Fort Belvoir, VA: Defense Technical Information Center, March 2003. http://dx.doi.org/10.21236/ada416620.

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Knudsen, Beatrice S. Hepatocyte Growth Factor and Interleukin-6 in Prostate Cancer Bone Metastasis. Fort Belvoir, VA: Defense Technical Information Center, June 2006. http://dx.doi.org/10.21236/ada467982.

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Naseem, Syed M., K. A. Mereish, Rikki Solow, and Harry Hines. Toxin-Induced Activation of Rat Hepatocyte Prostaglandin Synthesis and Phospholipid Metabolism. Fort Belvoir, VA: Defense Technical Information Center, April 1990. http://dx.doi.org/10.21236/ada221157.

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Knudsen, Beatrice S. Hepatocyte Growth Factor and Interleukin-6 in Prostate Cancer Bone Metastasis. Fort Belvoir, VA: Defense Technical Information Center, March 2004. http://dx.doi.org/10.21236/ada428439.

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Siegfried, Jill. Targeting the Hepatocyte Growth Factor Pathway for the Treatment of Breast Cancer. Fort Belvoir, VA: Defense Technical Information Center, September 1999. http://dx.doi.org/10.21236/ada383623.

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Young, Erin, Cem Kuscu, Christine Watkins, and Murat Dogan. Using CRISPR Gene Editing to Prevent Accumulation of Lipids in Hepatocytes. University of Tennessee Health Science Center, January 2022. http://dx.doi.org/10.21007/com.lsp.2022.0007.

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CRISPR gene editing is a molecular technology that can be used to silence gene expression. In this experiment, genes that are known to play a role in lipid accumulation in hepatocytes were targeted. Specifically, levels of fatty acid transport proteins 2 and 5 (FATP2 & 5) have been shown to be elevated in cases of non-alcoholic fatty liver disease. The goal of this experiment was to reduce expression of these genes by using a dead Cas9 (dCas9) protein with an attached inhibitory domain (KRAB) that acts on the promotor region. When measuring the mRNA expression, it was determined that the levels of the CRISPR-modified gene products were significantly reduced compared to the control. However, the same extent of inhibition was not consistently observed when conducting flow cytometry. Current work is aimed at discovering why lipid accumulation is not inhibited to the expected degree based on the results of mRNA expression.
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Ross, Bernard A. Adjuvant Action of Hepatocyte Growth Factor in Vitamin D Therapy of Androgen-Unresponsive Prostate Cancer. Fort Belvoir, VA: Defense Technical Information Center, December 2001. http://dx.doi.org/10.21236/ada401687.

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Lee, Young H. Mechanism of Hepatocyte Growth Factor Inhibition of Angiotensin II-induced Apoptosis in Primary Lung Cells. Fort Belvoir, VA: Defense Technical Information Center, January 2010. http://dx.doi.org/10.21236/ad1013415.

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Popov, Georgi, Aleksander Shkondrov, Magdalena Kondeva-Burdina, Vasil Manov, and Irena Krasteva. Effect of a Purified Saponins Mixture from Astragalus glycyphylloides on Rat Hepatocytes. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, May 2021. http://dx.doi.org/10.7546/crabs.2021.05.09.

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