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Ribalta, Teresa, Ian E. McCutcheon, Antonio G. Neto, Deepali Gupta, A. J. Kumar, David A. Biddle, Lauren A. Langford, Janet M. Bruner, Norman E. Leeds, and Gregory N. Fuller. "Textiloma (Gossypiboma) Mimicking Recurrent Intracranial Tumor." Archives of Pathology & Laboratory Medicine 128, no. 7 (July 1, 2004): 749–58. http://dx.doi.org/10.5858/2004-128-749-tgmrit.
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Abstract Context.—Resorbable substances used to achieve hemostasis during neurosurgical procedures comprise 3 principal classes based on chemical composition: (1) gelatin sponge, (2) oxidized cellulose, and (3) microfibrillar collagen. Nonresorbable hemostatic aides include various forms of cotton and rayon-based hemostats (cottonoids and kites). Resorbable and nonresorbable hemostatic agents have been reported to cause symptomatic mass lesions, most commonly following intra-abdominal surgery. Histologic examination typically shows a core of degenerating hemostatic agent surrounded by an inflammatory reaction. Each agent exhibits distinctive morphologic features that often permit specific identification. Hemostat-associated mass lesions have been variously referred to as textilomas, gossypibomas, gauzomas, or muslinomas. Objectives.—The aims of this study were to (1) identify cases of histologically proven cases of textiloma in neurosurgical operations, (2) characterize the specific hemostatic agent associated with textiloma formation, and (3) characterize the preoperative magnetic resonance imaging appearance of textiloma. Design.—Cases in which a textiloma constituted the sole finding on repeat surgery for recurrent brain tumor, or was a clinically significant component of a radiologically identified mass lesion together with residual tumor, constituted the study set. Results.—Five textilomas were identified and evaluated. The primary neoplasm was different in each case and included pituitary adenoma, tanycytic ependymoma, anaplastic astrocytoma, gliosarcoma, and oligodendroglioma. In all cases, preoperative magnetic resonance imaging suggested recurrent tumor. Textilomas included all categories of resorbable hemostatic agent. Other foreign bodies were present in some cases; the origin of these foreign bodies was traced to fibers shed from nonresorbable hemostatic material placed temporarily during surgery and removed before closure (cottonoids and kites). Inflammatory reactions included giant cells, granulomas, and fibroblastic proliferation. Microfibrillar collagen (Avitene) textilomas were associated with a striking eosinophil infiltration that was not seen with any other hemostatic agent. Conclusions.—Hemostatic agents may produce clinically symptomatic, radiologically apparent mass lesions. When considering a mass lesion arising after intracranial surgery, the differential diagnosis should include textiloma along with recurrent tumor and radiation necrosis.
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Wang, Qinghua, Jingwei Chen, Dexiang Wang, Minghui Shen, Huilong Ou, Jing Zhao, Ming Chen, Guoliang Yan, and Jun Chen. "Rapid Hemostatic Biomaterial from a Natural Bath Sponge Skeleton." Marine Drugs 19, no. 4 (April 15, 2021): 220. http://dx.doi.org/10.3390/md19040220.
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Uncontrolled bleeding is the main cause of mortality from trauma. Collagen has been developed as an important hemostatic material due to its platelet affinity function. A bath sponge skeleton is rich in collagen, also known as spongin. To understand the hemostatic effect of spongin, spongin materials, SX, SFM and SR were prepared from the bath sponge Spongia officinalis, and hemostatic experiments were performed. The SX, SFM and SR were significantly better than the positive control, type I collagen, in shortening the whole blood clotting time in vitro and hemostasis upon rat tail amputation. In a hemostatic experiment of rabbit common carotid artery injury, the hemostatic time and 3 h survival rate of the SFM group were 3.00 ± 1.53 min and 100%, respectively, which are significantly better than those of the commercial hemostat CELOX-A (10.33 ± 1.37 min and 67%, respectively). Additionally, the SFM showed good coagulation effects in platelet-deficient blood and defibrinated blood, while also showing good biocompatibility. Through a variety of tests, we speculated that the hemostatic activity of the SFM is mainly caused by its hyperabsorbency, high affinity to platelets and high effective concentration. Overall, the SFM and spongin derivates could be potential hemostatic agents for uncontrolled bleeding and hemorrhagic diseases caused by deficiency or dysfunction of coagulation factors.
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Sharma, Saurabh, Tejraj P. Kale, Lingaraj J. Balihallimath, and Abhishek Motimath. "Evaluating Effectiveness of Axiostat Hemostatic Material in achieving Hemostasis and Healing of Extraction Wounds in Patients on Oral Antiplatelet Drugs." Journal of Contemporary Dental Practice 18, no. 9 (2017): 802–6. http://dx.doi.org/10.5005/jp-journals-10024-2130.
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ABSTRACT Aim The aim of this study was to evaluate the efficacy of Axiostat Hemostatic Dental dressing in achieving hemostasis postextraction and determining its effect on pain and healing of the extraction wound, compared with control, i.e., conventional method of extraction in patients on oral antiplatelet therapy. Materials and methods Totally, 40 patients on oral antiplatelet drugs were included in the study and overall 80 extractions were done applying split mouth study design, without altering patient's drug regime. Extraction sites were divided into two groups: Group I received Axiostat Hemostatic Dental Dressing (study site), and group II received conventional method; pressure pack with sterile gauze under biting pressure followed by suturing if required (control site) was used to attain hemostasis. Results Extraction sites treated with Axiostat Hemostatic Dressing achieved hemostasis earlier (mean 1 minute 13 seconds) compared with control sites (mean = 14 minutes 1 second), which was also statistically significant (p < 0.001). Postoperative pain was considerably lower and significantly better healing was seen in the study group (p < 0.001) compared with the control. Conclusion Axiostat demonstrated to be an effective hemostatic agent that considerably lessens the bleeding time in patients on oral antiplatelet drugs postextraction. In addition, it even offered minimal postoperative pain and improved healing of the extraction wound. On comparing the results of this study with our study on HemCon Dental Dressing, Axiostat Dental Dressing (ADD) is found to be as effective and at par in achieving hemostasis in patients on oral antiplatelet therapy. Clinical significance The past few decades have seen an upsurge in use of low-dose aspirin either alone or in combination with other drugs. When these patients require dental/maxillofacial treatment, earlier concept of stopping these medications is associated with increased risk of thromboembolic event. The present study highlights an alternative approach using ADD which aids in quick hemostasis, accentuates healing, and reduce postoperative pain. How to cite this article Sharma S, Kale TP, Balihallimath LJ, Motimath A. Evaluating Effectiveness of Axiostat Hemostatic Material in achieving Hemostasis and Healing of Extraction Wounds in Patients on Oral Antiplatelet Drugs. J Contemp Dent Pract 2017;18(9):802-806.
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Nabavizadeh, Mohammad Reza, Arman Zargaran, Fariborz Moazami, Fatemeh Askari, Safoora Sahebi, Alireza Farhadpoor, and Pouya Faridi. "Comparison of the Hemostatic Activity of Quercus persica Jaub. & Spach. (Oak) With Ferric Sulfate in Bony Crypts." Journal of Evidence-Based Complementary & Alternative Medicine 21, no. 1 (June 30, 2015): 34–38. http://dx.doi.org/10.1177/2156587215593378.
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Effective tissue hemostasis in periapical surgical site is important in the procedures. Plants with large amount of tannins may act as a local hemostatic agent. We aimed to compare the hemostatic effect of the extract of Quercus persica with one of the common hemostatic material used in periapical surgery. Six standardized bone holes were prepared in the calvaria of 5 Burgundy rabbits. Two hemostatic medicaments were tested for their hemostatic effect and were compared with control defects: Group 1, cotton pellet soaked in 15.5% ferric sulfate solution; Group 2, cotton pellet soaked in pure ethanolic extract of Q. persica. Bleeding score between the groups was compared. The ferric sulfate group exhibited significantly less bleeding than the other 2 groups. Q. persica was found to cause more hemostasis than the control group at 4 and 5 minutes but there were no significant differences between normal saline and Q. persica extract in bleeding control.
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Zhang, Shiyao, Tingting Shi, Guangjun Wu, Qi Zhang, and Pibo Ma. "Preparation and property of soluble hemostatic material with 3D knitted structure." Journal of Industrial Textiles 52 (August 2022): 152808372211073. http://dx.doi.org/10.1177/15280837221107399.
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To solve the problems of low mechanical properties and poor coagulation effect of soluble hemostatic dressings, a new type of soluble hemostatic fabric with three-dimensional knitted structure is proposed in this paper. Three-dimensional knitted fabrics had good extension and porous structure. This three-dimensional soluble knitted fabric can be used for skin wounds clotting and healing. This paper mainly studied the influence of structural thickness on the performance of three-dimensional knitted dressings. Characterization analysis showed that three-dimensional knitted soluble hemostatic dressings had appropriate porosity and water vapor transmission rate (WVTR), and the fabric with the maximum thickness had good elongation (70.04%) and a very low blood clotting index (BCI) (11.18%). This suggests the potential application for soluble three-dimensional knitted structure fabric on skin wound hemostasis.
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Lin, Xiangwei, Yajing Shen, and Lidai Wang. "Multi-Scale Photoacoustic Assessment of Wound Healing Using Chitosan–Graphene Oxide Hemostatic Sponge." Nanomaterials 11, no. 11 (October 28, 2021): 2879. http://dx.doi.org/10.3390/nano11112879.
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Hemostasis is vital to save lives, reducing risks of organ failure and hemorrhagic shock. Exploring novel hemostatic materials and precise monitoring of the hemostatic status is of great importance for efficient hemostasis. We present the development of chitosan–graphene oxide-based hemostatic composite and multi-scale photoacoustic evaluation of the hemostatic performance. The hemostatic sponge can quickly and efficiently absorb the blood with its porous cavity and specific surficial property. We inspect the hemostatic performance via an in vitro blood absorption test and in vivo mouse bleeding injury experiments. Results show that the synthesized hemostatic sponge can not only absorb plasma in blood fast with its interior porous structure but also stimulate the interfacial reaction with erythrocytes and platelets. The superiority of multi-scale photoacoustic imaging for guiding, monitoring, and evaluating the hemostatic stages of sponges is demonstrated with high spatial resolution and great sensitivity at depths. Photoacoustic evaluation of a chitosan–graphene oxide-based hemostatic sponge has the potential to be transferred toward the clinical assessment of wound healing.
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Kerbl, Kurt, and Ralph V. Clayman. "Acute Hemostasis During Laparoscopic Procedures: Method for Intraoperative Application of Hemostatic Material." Journal of Urology 151, no. 1 (January 1994): 109–10. http://dx.doi.org/10.1016/s0022-5347(17)34883-8.
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Chen, Yeyi, Lei Wu, Pengpeng Li, Xiao Hao, Xiao Yang, Guanghui Xi, Wen Liu, Yakai Feng, Hongchao He, and Changcan Shi. "Polysaccharide Based Hemostatic Strategy for Ultrarapid Hemostasis." Macromolecular Bioscience 20, no. 4 (February 18, 2020): 1900370. http://dx.doi.org/10.1002/mabi.201900370.
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Li, Lin, Yan Du, Yi Xiong, Zhengwen Ding, Guoyu Lv, Hong Li, and Tielong Liu. "Injectable negatively charged gelatin microsphere-based gels as hemostatic agents for intracavitary and deep wound bleeding in surgery." Journal of Biomaterials Applications 33, no. 5 (October 18, 2018): 647–61. http://dx.doi.org/10.1177/0885328218807358.
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Gelatin, as natural macromolecular material, has been used in biomedical fields widely. In this study, various injectable gelatins A, B, and their compound AB microsphere-based gels (A-GMGs, B-GMGs and AB-GMGs) were prepared through water-in-oil emulsion method for hemostasis, and the effects of blood coagulation in vitro and surgical hemostasis (a deep liver wound model) in vivo were evaluated. Furthermore, the influences of gelatin sorts, the size of microsphere, zeta potential (ZP) and viscoelastic properties on hemostasis were also assessed. Results showed that the gelatin microspheres (GMs) exhibited smooth surface, good sphericity and the particle size of a rough normal distribution. GMs carried negative charges and their electronegativity was stronger than that of gelatin A (GA) and gelatin B (GB) raw materials. Rheological analysis showed that a decreasing particle size of the microspheres led to stronger gel strength, and solid-like gels were exhibited under low stress conditions and liquid-like gels were exhibited under high stress conditions. The blood clotting time of B-GMGs was within 60 s, which exhibited a significantly higher blood clotting effect compared with control groups. The hemostasis assay in vivo showed that the gels had better hemostatic effect on a deep liver wound bleeding model compared with control groups, especially B-GMGs. However, in vivo and vitro hemostatic experiments, particle size of GMs had no obvious influence on the hemostatic effect of the gels. In addition, the CCK-8 assay of bone marrow mesenchymal stem cells of murine (mMSCs) indicated non-cytotoxicity of GMs for cells. These results demonstrated that the gelatin microsphere-based gels (GMGs) had potential to be an effective hemostatic material for intracavitary and deep wound bleeding in surgery.
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Chen, Zihao, Lei Han, Changjun Liu, Yu Du, Xiao Hu, Ge Du, Chao Shan, et al. "A rapid hemostatic sponge based on large, mesoporous silica nanoparticles and N-alkylated chitosan." Nanoscale 10, no. 43 (2018): 20234–45. http://dx.doi.org/10.1039/c8nr07865c.
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Due to the designed, coagulation-promoting microstructure, a rapid and safe hemostat was developed and its hemostatic efficiency was evaluated by in vitro clotting tests and in vivo hemostatic analyses.
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Tigchelaar, Izaak, Stefan Monnink, Johan Haan, Noriyuki Tabuchi, and Willem Van Oeveren. "Platelet Function Analysis After In Vitro Treatment with Hemostasis-Affecting Components." Seminars in Thrombosis and Hemostasis 21, S 02 (March 1995): 71–76. http://dx.doi.org/10.1055/s-0032-1313606.
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An overall platelet function test in whole blood, which simulates conditions under arterial pressure, is useful in measuring the effect of polymer materials on blood hemostatic function. We performed biocompatibility tests with materials or plasma substitutes by interaction of blood from healthy volunteers and then subjected these blood samples to platelet function analysis (Thrombostat). We tested also the capacity of locally applied hemostatic agents for bleeding control by direct application of these agents onto the Thrombostat measuring cell. The biocompatibility tests with materials exposed to blood appeared very discriminating between compatible and noncompatible materials. The hemostatic capacity of blood exposed to noncompatible materials (assessed by binding of active thrombin) reduced markedly after one hour incubation of the material. The plasma substitutes did not affect hemostasis significantly. However, a blood dilution of 40%, as in cardiopulmonary bypass, increased the time required for closure of the measuring cell by a platelet plug exponentially. Local hemostatic agents could be selected according to their capacity to enhance platelet plug formation. In addition, ADP mixed with the hemostatic agent was most effective in improving capacity. We conclude that platelet function analysis contributes importantly to screening of materials and plasma substitutes with regard to their interaction with primary hemostasis.
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Stenson, Katherine M., Ian M. Loftus, Ian Chetter, Inge Fourneau, Stephen Cavanagh, Colin Bicknell, and Paros Loftus. "A Multi-Centre, Single-Arm Clinical Study to Confirm Safety and Performance of PuraStat®, for the Management of Bleeding in Elective Carotid Artery Surgery." Clinical and Applied Thrombosis/Hemostasis 28 (January 2022): 107602962211443. http://dx.doi.org/10.1177/10760296221144307.
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Anastomotic bleeding in vascular surgery can be difficult to control. Patients, in particular those undergoing carotid surgery, have often been started on treatment with dual antiplatelet agents and receive systemic heparinization intraoperatively. The use of local hemostatic agents as an adjunct to conventional methods is widely reported. 3-D Matrix’s absorbable hemostatic material RADA16 (PuraStat®), is a fully synthetic resorbable hemostatic agent. The aim of this study is to confirm the safety and performance of this agent when used to control intraoperative anastomotic bleeding during carotid endarterectomy (CEA). A prospective, single-arm, multicenter study involving 65 patients, undergoing CEA, in whom the hemostatic agent was applied to the suture line after removal of arterial clamps. Patients were followed up at 24 h, discharge, and one month after surgery. Time to hemostasis was measured as the primary endpoint. Secondary endpoints included hemostasis efficacy and safety outcomes, blood loss, intraoperative and postoperative administration of blood products, and incidence of reoperation for bleeding. A total of 65 cases (51 male and 14 female) undergoing CEA, utilizing patch reconstruction (90. 8%), eversion technique (6.1%), and direct closure (3.1%) were analyzed. All patients received dual antiplatelet therapy preoperatively and were administered systemic intravenous heparin intraoperatively, as per local protocol. The mean time to hemostasis was 83 s ± 105 s (95% CI: 55-110 s). Primary hemostatic efficacy was 90.8%. The mean volume of product used was 1.7 mL ± 1.1 mL. Hemostasis was achieved with a single application of the product in 49 patients (75.3%). Two patients required a transfusion of blood products intraoperatively. There were no blood product transfusions during the postoperative period. The intraoperative mean blood loss was 127 mL ± 111.4 mL and postoperatively, the total mean drainage volume was 49.0 mL ± 51.2 mL. The mean duration of surgery was 119 ± 35 min, and the mean clamp time was 35 min 12 s ± 19 min 59 s. In 90.8% of patients, there was no presence of hematoma at 24 h postoperatively. Three returned to theatre due to bleeding (2 in the first 24 h), however, none of these cases were considered product related. Overall, there were no device-related serious adverse events (SAE) or unanticipated device-related SAEs reported. Use of the hemostatic agent PuraStat® is associated with a high rate of hemostatic efficacy (90.8%) and a short time to hemostasis. The safety of the product for use on vascular anastomoses has been demonstrated.
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Hwang, Yeok Gu, Jin Woo Lee, Eun Ae Won, and Seung Hwan Han. "Prospective Randomized Controlled Study of Hemostatic Efficacy with Kaolin-Impregnated Dressings in Diabetic Foot Ulcers Taking Anticoagulants Undergoing Debridement in an Outpatient Clinic." Journal of Diabetes Research 2019 (November 11, 2019): 1–6. http://dx.doi.org/10.1155/2019/9316380.
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Background. The effective hemostasis after minor debridement in an outpatient clinic is important and essential. This study is aimed at evaluating the hemostatic efficacy and safety of the kaolin-impregnated dressing for diabetic foot ulcer patient who take an anticoagulant. Methods. A prospective, randomized, clinical trial study was performed in twenty-six patients with diabetic foot ulcers who take an anticoagulant requiring minor debridement in an outpatient clinic. Minor debridement and removal of break down skin were performed by one orthopedic surgeon. Hemostasis on wound bed was achieved using kaolin-impregnated gauze (study group) and standard sterilized dry gauze (control group). Two randomized groups were compared for hemostatic efficacy and side effect. Results. For the purpose of this study, the presence or absence of hemostasis was assessed at 5 and 10 minutes after the application of dressing material. Treatment was evaluated as successful if bleeding was ceased adequately, and no extra hemostatic measures were required within 10 minutes. At 5 minutes, 80% of patients using the kaolin-impregnated gauze successfully achieved complete hemostasis versus 40% in the control group that controlled bleeding partially. With kaolin-impregnated gauze, 100% of patients show complete hemostasis at 10 minutes versus 58.3% in the standard gauze (P<.001). An adverse effect was not noted in all patients. Conclusions. The use of kaolin-impregnated gauze appears to be a safe and feasible option in managing bleeding after debridement of diabetic foot ulcers, and merits to patient who had a bleeding tendency is high. This trial is registered with CRIS registration number KCT0003894.
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Zhu, Xulong, Jianxiong Wang, Shuhan Wu, Tian Liu, Guangshuai Lin, Bin Shang, Jia Ma, et al. "Biological Application of Novel Biodegradable Cellulose Composite as a Hemostatic Material." Mediators of Inflammation 2022 (August 10, 2022): 1–8. http://dx.doi.org/10.1155/2022/4083477.
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Degradable hemostatic materials have unique advantages in reducing the amount of bleeding, shortening the surgical operation time, and improving patient prognosis. However, none of the current hemostatic materials are ideal and have disadvantages. Therefore, a novel biodegradable cellulose-based composite hemostatic material was prepared by crosslinking sodium carboxymethyl cellulose (CCNa) and hydroxyethyl cellulose (HEC), following an improved vacuum freeze-drying method. The resulting cellulose composite material was neutral in pH and spongy with a density of 0.042 g/cm3, a porosity of 77.68%, and an average pore size of 13.45 μm. The composite’s compressive and tensile strengths were 0.1 MPa and 15.2 MPa, respectively. Under in vitro conditions, the composites were degraded gradually through petite molecule stripping and dissolution, reaching 96.8% after 14 days and 100% degradation rate at 21 days. When implanted into rats, the degradation rate of the composite was slightly faster, reaching 99.7% in 14 days and 100% in 21 days. Histology showed a stable inflammatory response and no evidence of cell degeneration, necrosis, or abnormal hyperplasia in the tissues around the embedded material, indicating good biocompatibility. In the hemorrhagic liver model, the time to hemostasis and the total blood loss in the cellulose composite group was significantly lower than in the medical gauze group and the blank control group ( P < 0.05 ). These data indicate that the novel cellulose composite is a promising implantable hemostatic material in clinical settings.
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Suchý, Pavel, Alice Paprskářová, Marta Chalupová, Lucie Marholdová, Kristina Nešporová, Jarmila Klusáková, Gabriela Kuzmínová, Michal Hendrych, and Vladimír Velebný. "Composite Hemostatic Nonwoven Textiles Based on Hyaluronic Acid, Cellulose, and Etamsylate." Materials 13, no. 7 (April 1, 2020): 1627. http://dx.doi.org/10.3390/ma13071627.
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The achievement of rapid hemostasis represents a long-term trend in hemostatic research. Specifically, composite materials are now the focus of attention, based on the given issues and required properties. In urology, different materials are used to achieve fast and effective hemostasis. Additionally, it is desirable to exert a positive influence on local tissue reaction. In this study, three nonwoven textiles prepared by a wet spinning method and based on a combination of hyaluronic acid with either oxidized cellulose or carboxymethyl cellulose, along with the addition of etamsylate, were introduced and assessed in vivo using the rat partial nephrectomy model. A significantly shorter time to hemostasis in seconds (p < 0.05), was attributed to the effect of the carboxymethyl cellulose material. The addition of etamsylate did not noticeably contribute to further hemostasis, but its application strengthened the structure and therefore significantly improved the effect on local changes, while also facilitating any manipulation by the surgeons. Specifically, the hyaluronic acid supported the tissue healing and regeneration, and ensured the favorable results of the histological analysis. Moreover, the prepared textiles proved their bioresorbability after a three-day period. In brief, the fabrics yielded favorable hemostatic activity, bioresorbability, non-irritability, and had a beneficial effect on the tissue repair.
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Zhang, Xiaojian, Kaili Dai, Chenyu Liu, Haofeng Hu, Fulin Luo, Qifan Qi, Lei Wang, et al. "Berberine-Coated Biomimetic Composite Microspheres for Simultaneously Hemostatic and Antibacterial Performance." Polymers 13, no. 3 (January 22, 2021): 360. http://dx.doi.org/10.3390/polym13030360.
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Biomimetic microspheres containing alginate/carboxymethylcellulose/gelatin and coated with 0%, 1%, 3%, and 6% berberine (BACG, BACG-1B, BACG-3B, BACG-6B) were prepared by the oil-in-water emulsion method combined with spray drying. Through a series of physicochemical parameters and determination of hemostatic properties in vitro and in vivo, the results indicated that BACG and BACG-Bs were effective in inducing platelet adhesion/aggregation and promoting the hemostatic potential due to their biomimetic structure and rough surface. In addition, BACG-6B with high berberine proportion presented better hemostatic performance compared with the commercial hemostatic agent compound microporous polysaccharide hemostatic powder (CMPHP). BACG-6B also showed strong antibacterial activity in the in vitro test. The hemolysis test and cytotoxicity evaluation further revealed that the novel composite biomaterials have good hemocompatibility and biocompatibility. Thus, BACG-6B provides a new strategy for developing a due-functional (hemostat/antibacterial) biomedical material, which may have broad and promising applications in the future.
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Shin, Mikyung, Jae Hyuk Choi, Keumyeon Kim, Soomi Kim, and Haeshin Lee. "Hemostatic Needles: Controlling Hemostasis Time by a Catecholamine Oxidative Pathway." ACS Applied Materials & Interfaces 13, no. 9 (February 23, 2021): 10741–47. http://dx.doi.org/10.1021/acsami.0c22223.
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Kadyseva, Oksana Vladimirovna, Vladimir Nikolaevich Bykov, Olga Yurievna Strelova, and Alexander Nikolaevich Grebenyuk. "Determination of indicators allowing to evaluating the hemostatic activity of chitosan without a biological experiment." Journal of Bioactive and Compatible Polymers 36, no. 4 (July 2021): 276–82. http://dx.doi.org/10.1177/08839115211030607.
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The biopolymer chitosan is widely used for the development of local hemostatic agents. However, the physicochemical parameters of chitosan that determine its hemostatic properties have not yet been determined. Standard quality control of chitosan-containing raw materials and medical products on its basis do not allow us to make a conclusion about the effectiveness of their use for stopping bleeding. The most reliable method for assessing hemostatic activity remains in vivo experiment on large animals. The aim of this study was to determine additional physicochemical parameters of chitosan, which would make it possible to predict its hemostatic activity without conducting a biological experiment. In this work, using the methods of nuclear magnetic resonance spectroscopy, spectrophotometry and viscometry, it has been shown that the ability to initiate hemostasis is depending of the molecular weight and degree of deacetylation of chitosan, but not enough linearly. The hemostatic properties in vitro increases in a series of samples with a relatively constant molecular weight with an increase in the degree of deacetylation. As well as in a series with the same degree of deacetylation with an increase in molecular weight. However, at molecular weight values more than 300 kDa, the viscosity of the polymer causes the opposite effect: with an increase in the degree of deacetylation, the hemostatic activity decreases. The best ability to initiate hemostasis have chitosan samples with a degree of deacetylation of 90.0%–97.4% and molecular weight 145.7–284.7 kDa, in which at pH of solution close to physiological, a significant part of the molecules transitioned from conformation state rigid rod to state globule. It was accompanied by an abrupt change in light transmission of the solution. It was concluded, that it is possible to study conformational states by spectrophotometry to assess the hemostatic activity of chitosan samples without performing biological experiment.
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Jovanovic, Milan, Goran Stanojevic, Jovica Jovanovic, and Miroslav Stojanovic. "Splenic capping in the treatment of severe spleen injuries." Medical review 58, no. 7-8 (2005): 388–92. http://dx.doi.org/10.2298/mpns0508388j.
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Introduction Permanent and lifelong threat of developing fatal sepsis and other severe infections in splenectomized patients is an important reason for promoting splenic preservation. Material and methods Experimental preservation of severe spleen injuries (grade IV), with splenic-capping, was performed in 30 dogs, operated using Ketalar anesthesia. Spleen preservation and concomitant treatment of excessive hemorrhage were achieved by highly controlled "conic" compression of injuried spleen parenchyma, additional hemostatic Z-sutures and fibrin tissue adhesion. Results Satisfactory hemostasis and complete preservation of the spleen parenchyma damage were achieved in 20% bv splenic capping splenorraphy, alone. Additional placement of hemostatic sutures was necessary in 30%, and concomitant application of serum thymic factor (STF) FTA (along with sutures) in 50% of patients. The abovementioned techniques were very effective in achieving hemostasis (100%), with no mortality and low rate of postoperative complications - 6.7% (1 intrasplenic abscess and 1 adhesive ileus). Conclusion Excellent results and efficacy, splenic capping-splenorraphy is the method of choice in the treatment of severe and multiple transcapsular spleen injuries with involvement of segmental vessels.
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Park, Young-Mok, Hyung-Il Seo, Jae-Hoon Kim, Sung Pil Yoon, Haeshin Lee, and Moon Sue Lee. "Clinical application of a new hemostatic material using mussel-inspired catecholamine hemostat: A pilot study." Annals of Hepato-Biliary-Pancreatic Surgery 26, no. 1 (February 28, 2022): 98–103. http://dx.doi.org/10.14701/ahbps.21-077.
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Ma, Fei, Shujing Sui, Zhiyuan Yang, Tong Ye, Lei Yang, Peng Han, Hui Gan, et al. "Evaluation of Novel Tranexamic Acid/Montmorillonite Intercalation Composite, as a New Type of Hemostatic Material." BioMed Research International 2022 (February 28, 2022): 1–15. http://dx.doi.org/10.1155/2022/3963681.
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Radiation enteritis—clinically manifested as diarrhea, intestinal bleeding, and so on—is frequently caused when the body is exposed to radiation or radiotherapy because the intestine is radiation-sensitive as an abdominal organ. Therefore, strategies to modulate intestinal hemostasis had inspired an important research trend in the process of preventing and treating radiation enteritis. Based on the structural characteristics of montmorillonite (MMT) and the hemostatic drug tranexamic acid (TXA) which was used clinically to treat enteritis, the tranexamic acid-montmorillonite composite material (TXA-MMT) was prepared through intercalation composite technology. According to the analysis of FTIR, XRD, TG-DTG, SEM, and XRF, the prepared TXA-MMT was verified that tranexamic acid could intercalate into layers of montmorillonite. To evaluate the biocompatibility, two experiments were conducted by in vitro hemolysis and in vitro cytotoxicity experiments and results showed that TXA-MMT exhibited good visible biocompatibility. Activated partial thromboplastin time, prothrombin time, and in vitro clotting time were adopted to determine the hemostatic effect of TXA-MMT. Compared with other groups, TXA-MMT revealed a significant decrease in clotting time variations, APTT, and PT. In addition, to investigate the preventive effect of TXA-MMT by the intervention of radiation enteritis mice, inflammatory factors IL-1β, IL-6, and TNF-α and the content of endotoxin in the serum of mice were detected. It demonstrated that TXA-MMT reduced the levels of these factors. Besides, the expression and the pathological changes of the small intestine tissue of mice were relieved. Our findings suggests that TXA-MMT as a promising intercalation composite has a great potential for application in the field of intestinal hemostasis.
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Liu, Jiaxi, Xiang Zhou, Yin Zhang, Wei Zhu, Anping Wang, Meijia Xu, and Shuxian Zhuang. "Rapid hemostasis and excellent antibacterial cerium-containing mesoporous bioactive glass/chitosan composite sponge for hemostatic material." Materials Today Chemistry 23 (March 2022): 100735. http://dx.doi.org/10.1016/j.mtchem.2021.100735.
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Khabadze, Z. S., D. A. Nazarova, E. S. Shilyaeva, A. P. Kotelnikova, Yu A. Bakayev, S. M. Abdulkerimova, and Kh O. Omarova. "Hemostatic agents in periapical surgery: The systematic review." Endodontics Today 19, no. 3 (October 16, 2021): 184–87. http://dx.doi.org/10.36377/1683-2981-2021-19-3-184-187.
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Several agents have been used to secure hemostasis during periapical surgery. Their efficacy, biological response and side effects differ from each other.Aim. Of this review article is to assess systematically the available scientific evidence about the clinical response after using hemostatic agents during apical surgery.Materials and methods. The study of publications was produced in the electronic databases such as Google Scholar, PubMed during a systematic review of the literature. Included articles contain information about using hemostatic agents during periapical surgery and their adverse effects. The publication date criterion was selected from January 2006 to September 2021.Results. 55 articles were viewed during the review. After analyzing the literature for inclusion criteria, the total number of publications has become 10.Conclusions. According to literature data, different hemostatic agents are used during periapical surgery, but there weren’t any inflammatory reactions while using calcium sulfate.
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Cheng, Yu, Qian Yang, Jiyuan Wang, Zhang Hu, Chengpeng Li, Saiyi Zhong, and Na Huang. "Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge." Polymers 15, no. 3 (January 28, 2023): 672. http://dx.doi.org/10.3390/polym15030672.
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Considering the great harm to the human body caused by severe and massive bleeding, in this study, chitosan-grafted norfloxacin (CTS-NF) composites were prepared with chitosan (CTS) and norfloxacin (NF) as raw materials by a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-mediated coupling method to solve the limitations of slow hemostatic and poor anti-infective effects of current dressings on the market. The effects of the mass ratio of CTS to NF (MCTS/MNF), reaction temperature T and reaction time t on the grafting rate (η%) of the products were investigated through single factor tests. The preparation process was optimized with the η% as an evaluation index by means of the Box–Behnken test design and response surface analysis. The antimicrobial activity was evaluated by inhibition zone assay, and the hemostatic activity of the prepared composites was evaluated in vitro and in vivo. The results suggested that the optimum preparation conditions were the mass ratio of CTS to NF (MCTS/MNF) 5:3, reaction temperature 65 °C, and reaction time 4 h. Under this condition, the η% of CTS-NF was 45.5%. The CTS-NF composites displayed significant antimicrobial activities. Moreover, in vitro hemostasis results revealed that the CTS-NF composite had a lower blood clotting index and absorbed red blood cells to promote aggregation. In vivo ear and live hemostasis, the CTS-NF groups showed short hemostatic time (49.75 ± 3.32 s and 50.00 ± 7.21 s) and more blood loss (0.07 ± 0.010 g and 0.075 ± 0.013 g). The results showed that CTS-NF reduced the bleeding time and volume, exhibiting a significant coagulation effect. Therefore, the CTS-NF sponge is expected to be a new, effective hemostatic and antibacterial material in the future.
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Ruzibayev, R. Yu, P. Yu Ruzmatov, and D. A. Umarov. "Morphological Assessment Of Wounds Healing In The Region Of Anal Canal In Rats On The Background Of Medicamental Hypocoagulation." American Journal of Applied sciences 03, no. 09 (September 30, 2021): 5–15. http://dx.doi.org/10.37547/tajas/volume03issue09-02.
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Purpose of the study: to conduct a morphological study of experimental material to assess the features of wound healing in the anal sphincter area when using the domestic drug “Heprocel” as a hemostatic agent. Material and methods. The study was carried out in the Department of experimental surgery and the department of pathological anatomy of the Republican Specialized Scientific and Practical Medical Center for Surgery named after Academician V. Vakhidov. The state of experimental models of wounds with the application of a hemostatic agent in the form of a gel was studied. The experiments were carried out on 72 white male rats weighing 210-250 g. Two series of 36 animals each were produced. All animals were divided into groups: norm - experiment in the absence of drug hypocoagulation; control - an experiment with intravenous heparin to achieve drug hypocoagulation; experience - an experiment with intravenous administration of heparin to achieve drug hypocoagulation using an application on a model of a skin wound and a dissected anal sphincter of the hemostatic agent "Heprocel". Results. Morphological differences in the process of wound healing were most pronounced after 3 days of the study. In the control group of animals with hemostasis using an electrocoagulator, necrotic and exudative processes of inflammation in the stratified squamous epithelium and mucous membrane of the glands in the anal region were evident. In the experimental group, on the 3-5th day of the experiment, the proliferative process of inflammation predominated morphologically. A special feature is that the hemostatic gel is absorbed into the wound, forming a thin uniform layer. On the 10th day of the experiment, the above difference became more obvious. In the control group, chaotic squamous epithelial cells and fibroblasts were formed. The fibrin-necrotic layer is preserved. Focal infiltration of macrophages-lymphocytes was observed. In the experimental group, the intestinal layers began to change. In the area of the surgical incision, the hemostatic layer is preserved; in this area, the growth of thin fibrous connective tissue is observed. Differentiation and transformation of cells led to the regeneration of the epithelial layer and parakeratosis of the flat cell. Signs of complete remodeling were evident over a long period of time, such as 14 days. Conclusion. The use of a hemostatic agent in the form of a gel provides not only an increase in the processes of local hemostasis, but a decrease in the activity of inflammatory reactions and infection, as well as the risk of developing post-traumatic cicatricial deformity of the anus.
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Hu, Zhang, Sitong Lu, Yu Cheng, Songzhi Kong, Sidong Li, Chengpeng Li, and Lei Yang. "Investigation of the Effects of Molecular Parameters on the Hemostatic Properties of Chitosan." Molecules 23, no. 12 (November 30, 2018): 3147. http://dx.doi.org/10.3390/molecules23123147.
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Hemorrhea is one of the major problems in war, trauma care, and surgical operation that threaten the life of the injured and patients. As a novel polymeric hemostatic agent, biodegradable chitosan can stop bleeding through a variety of approaches. In this paper, chitosan with various molecular parameters was prepared from chitin as raw material through deacetylation, oxidative degradation, hydrophilic modification, and salt formation reactions. The influence of different polymer parameters on the hemostatic effects of chitosan was investigated by in vitro coagulation time and dynamic coagulation assay. The results showed that when the molecular weights were high (105–106) and approximate, the coagulation effect of chitosan improved with a decrease of the deacetylation degree and achieved a prominent level in a moderate degree of deacetylation (68.36%). With the same degree of deacetylation, the higher the molecular weight of chitosan, the better the procoagulant effect. The substituent derivatives and acid salts of chitosan showed significant procoagulant effects, especially the acid salts of chitosan. In addition, the hemostasis mechanism of chitosan with various parameters was preliminarily explored by analyzing the plasma recalcification time (PRT). The efforts in this paper laid a basis for further study of the structure–activity relationship and the mechanism of chitosan hemostasis.
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Hwang, Yeokgu, Yoo Jung Park, Dong-Woo Shim, Jin Woo Lee, and Seung Hwan Han. "Clinical Efficacy of a Kaolin-impregnated Dressing for Hemostatic Control in Diabetic Foot Ulcer Patients Receiving Anticoagulant Therapy in an Outpatient Clinic." Foot & Ankle Orthopaedics 2, no. 3 (September 1, 2017): 2473011417S0002. http://dx.doi.org/10.1177/2473011417s000213.
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Category: Diabetes Introduction/Purpose: Orthopedic physicians encounter many diabetes patients who receive anticoagulant treatment because vascular diseases are the chief causes of death and disability in these patients. In these patients, if surgery or other procedures are required, it is still unclear whether anticoagulation therapy administration should continue. After discontinuation of anticoagulation therapy, the risk of cardiovascular complications is increased, although complications of bleeding after surgery are decreased; this is a dilemma in the treatment of diabetic foot ulcer. The purpose of this study was to examine the effectiveness, safety, and hemostatic effect of kaolin-impregnated gauze on diabetic foot ulcer patients who continue to receive anticoagulants after surgical debridement. Methods: Twenty patients receiving anticoagulant medication with diabetic foot ulcers requiring surgical debridement were enrolled. Diabetic foot ulcers were treated using surgical debridement and a basic dressing protocol, which involved direct application of the dressing material to the wound bed. For the study group, a kaolin-impregnated dressing was used, and dry gauze was applied to patients in the control group. Patients were randomly assigned to either group, and we compared hemostatic efficacy and adverse effects between the two groups. The presence or absence of hemostasis was assessed 5 and 10 minutes after dressing application. Treatment was considered successful if bleeding ceased adequately and no extra hemostatic measures were required within 10 minutes. Results: In the 20 patients undergoing surgical debridement, there was no evidence of bleeding within 10 minutes of dressing application. Eight of the 10 patients treated with a kaolin-impregnated dressing achieved complete hemostasis within 5 minutes. Five of the 10 patients in the control group failed to achieve hemostasis within 10 minutes. No adverse effects were noted. Conclusion: The use of a kaolin-impregnated dressing appears to be a safe, feasible, and beneficial option for the management of diabetic foot ulcer patients with a high risk of bleeding.
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Qin, Xiaolei, Jean Felix Mukerabigwi, Mingzi Ma, Ruyi Huang, Mengdi Ma, Xueying Huang, Yu Cao, and Yang Yu. "In situ photo-crosslinking hydrogel with rapid healing, antibacterial, and hemostatic activities." e-Polymers 21, no. 1 (January 1, 2021): 606–15. http://dx.doi.org/10.1515/epoly-2021-0062.
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Abstract Uncontrollable bleeding is still the main cause of post-traumatic deaths due to the blood loss. Moreover, infectious complication of wound is also still a challenging problem for wound healing. Nevertheless, the currently available hemostasis drugs or materials cannot stanch bleeding well due to single function, slow in effectiveness, adhere to wounds easily, poor gas permeability, etc. Therefore, it is of a great significance to utilize a biomedical hemostatic material that can stop bleeding quickly, preventing from bacterial infections, and with good biocompatibility properties. Herein chitosan (CS) was modified with gallic acid (GA) and thrombin (TB) to prepare an antibacterial hemostatic composite dressing. The CS-based composite hydrogel dressing was obtained by acylation modification, ultraviolet curing crosslinking method and physical mixing. The in vitro results showed that our prepared CS-based composite hydrogel has obvious burst release and good degradation property. Moreover, the in vivo results showed that it has a strong antibacterial property that is much better than single CS, and it can stop bleeding in 1 min which can promote wound healing. Therefore, the findings of this study is expected to contribute to the future designing of biomedical hemostatic materials with improved properties.
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Darvin, V. V., A. Ya Ilkanich, M. G. Ryzhikov, A. V. Oganian, and A. V. Satinov. "Modern management of acute non-variceal upper gastrointestinal bleeding." Сибирский научный медицинский журнал 41, no. 6 (December 25, 2021): 4–17. http://dx.doi.org/10.18699/ssmj20210601.
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Acute, non-varicose bleeding from the upper gastrointestinal tract remains a common clinical problem. Bleeding episodes are associated with high mortality and a significant economic burden on the health care system. Despite the continuing improvement in endoscopic treatment, recurrent bleeding and associated mortality are still a pressing issue. In addition to the well-established modalities of endoscopic hemostasis: injection, thermal, mechanical, used both as mono therapy and as part of a combination therapy, the review discusses the use of novel types of endoscopic devices. The results of the use of over-the-scope clips, coagrasper, hemostatic sprays, endoscopic angiography, radiofrequency ablation, cryotherapy and endoscopic suturing device are described. The technical aspects of their application, the issues of efficacy and safety, the advantages and limitations of methods for achieving final endoscopic hemostasis are considered. The methods of initial assessment and treatment strategies for recurrent bleeding and unsuccessful endoscopic hemostasis were also analyzed. Material and methods. Literature search was carried out using the following electronic information resources: CyberLeninka, PubMed, Nature Pathology, MEDLINE, PLoS ONE. Results. Сombined endoscopic hemostasis is a standard therapy in the treatment of gastrointestinal tract cerebral infections with an efficiency of 95-98 %, new modalities of endoscopic hemostasis are able, in some cases, to achieve final endoscopic hemostasis both in primary endoscopic treatment and in recurrent bleeding.
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Kosayev, J. V., I. A. Khasanov, N. S. Abushov, and G. T. Taghi-zade. "The state of lipid exchange, hemostasis, inflammatory reaction and potentials for their correction in indirect revascularization in patients with critical ischemia of lower extremities (a correlation statistical analysis)." Laser Medicine 25, no. 1 (August 10, 2021): 27–35. http://dx.doi.org/10.37895/2071-8004-2021-25-1-27-35.
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Aim: to study the state of lipid metabolism, hemostasis, inflammatory reaction and the potential for their correction after indirect revascularization in patients with distal steno-occlusion of arteries and critical ischemia of lower extremities (critical ILE).Material and methods. Changes in hemostasis and dynamics of its parameters during the complex surgical treatment in 131 patients with critical ILE and distal arterial stenoocclusion were analyzed. To achieve the targeted goals, patients were divided into the following groups: 34 patients had traditional care (control group); 32 patients had intravenous laser blood irradiation in combination with standard therapy (Group I); 32 patients had cytokine therapy with roncoleukin in combination with standard therapy (Group II); 33 patients had intravenous laser blood irradiation combined with cytokine therapy and standard therapy (Group III). Parameters of lipid metabolism were studied in dynamics (total cholesterol, very low density lipoproteins, high density lipoproteins, triglycerides); products of lipid peroxidation (malondialdehydes, conjugates, superoxide dismutase); inflammatory mediators (C-reactive protein, sialic acids, seromucoids, fibrinogen A, circulating immune complexes); hemostatic parameters (fibrinogen, fibrinolytic activity, fibrin degradation products, antithrombin III activity). Hemostatic indices were compared with identical parameters of 48 apparently healthy individuals (reference group).Results. On admission, patients with critical ILE and distal wall occlusion had sharp changes in their lipid metabolism, inflammatory reaction, and hemostasis. Conclusion. The inclusion of intravenous laser blood irradiation and cytokine therapy separately and in combination in a set of therapeutic measures led to the leveling of the studied homeostasis indicators. The best results were obtained in the group where patients had combined perioperative intravenous laser blood irradiation with cytokine therapy in indirect revascularization.
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Edwards, J. Vincent, Elena Graves, Nicolette Prevost, Brian Condon, Dorne Yager, Joseph Dacorta, and Alvin Bopp. "Development of a Nonwoven Hemostatic Dressing Based on Unbleached Cotton: A De Novo Design Approach." Pharmaceutics 12, no. 7 (June 30, 2020): 609. http://dx.doi.org/10.3390/pharmaceutics12070609.
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Minimally processed greige (unbleached) cotton fibers demonstrate enhanced clotting relative to highly processed United States Pharmacopeia (USP) type 7 bleached cotton gauze. This effect is thought to be due to the material surface polarity. We hypothesized that a textile could be constructed, conserving the hemostasis-accelerating properties of greige cotton, while maintaining structural integrity and improving absorbance. Spun bond nonwovens of varying surface polarity were designed and prepared based on ratios of greige cotton/bleached cotton/polypropylene fibers. A thromboelastographic analysis was performed on fibrous samples in citrated blood to evaluate the rate of fibrin and clot formation. Lee White clotting times were obtained to assess the material’s clotting activity in platelet fresh blood. An electrokinetic analysis of samples was performed to analyze for material surface polarity. Hemostatic properties varied with composition ratios, fiber density, and fabric fenestration. The determinations of the surface polarity of cotton fabrics with electrokinetic analysis uncovered a range of surface polarities implicated in fabric-initiated clotting; a three-point design approach was employed with the combined use of thromboelastography, thrombin velocity index, Lee White clotting, and absorption capacity determinations applied to fabric structure versus function analysis. The resulting analysis demonstrates that greige cotton may be utilized, along with hydrophilic and hydrophobic fibers, to improve the initiation of fibrin formation and a decrease in clotting time in hemostatic dressings suitable to be commercially developed. Hydroentanglement is an efficient and effective process for imparting structural integrity to cotton-based textiles, while conserving hemostatic function.
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Wang, Lei, Wenjun Li, and Song Qin. "Three Polymers from the Sea: Unique Structures, Directional Modifications, and Medical Applications." Polymers 13, no. 15 (July 28, 2021): 2482. http://dx.doi.org/10.3390/polym13152482.
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With the increase of wounds and body damage, the clinical demand for antibacterial, hemostatic, and repairable biomaterials is increasing. Various types of biomedical materials have become research hotspots. Of these, and among materials derived from marine organisms, the research and application of alginate, chitosan, and collagen are the most common. Chitosan is mainly used as a hemostatic material in clinical applications, but due to problems such as the poor mechanical strength of a single component, the general antibacterial ability, and fast degradation speed research into the extraction process and modification mainly focuses on the improvement of the above-mentioned ability. Similarly, the research and modification of sodium alginate, used as a material for hemostasis and the repair of wounds, is mainly focused on the improvement of cell adhesion, hydrophilicity, degradation speed, mechanical properties, etc.; therefore, there are fewer marine biological collagen products. The research mainly focuses on immunogenicity removal and mechanical performance improvement. This article summarizes the source, molecular structure, and characteristics of alginate, chitosan, and collagen from marine organisms; and introduces the biological safety, clinical efficacy, and mechanism of action of these materials, as well as their extraction processes and material properties. Their modification and other issues are also discussed, and their potential clinical applications are examined.
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Ellis-Behnke, Rutledge G., Yu-Xiang Liang, David K. C. Tay, Phillis W. F. Kau, Gerald E. Schneider, Shuguang Zhang, Wutian Wu, and Kwok-Fai So. "Nano hemostat solution: immediate hemostasis at the nanoscale." Nanomedicine: Nanotechnology, Biology and Medicine 2, no. 4 (December 2006): 207–15. http://dx.doi.org/10.1016/j.nano.2006.08.001.
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Shkatula, Y. V., Y. O. Badion, and M. V. Novikov. "Efficiency of Different Methods of Temporary External Hemostasis at the Pre-Hospital Stage of Emergency Medical Care." Novosti Khirurgii 28, no. 6 (December 24, 2020): 688–93. http://dx.doi.org/10.18484/2305-0047.2020.6.688.
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Objective. To estimate the efficiency of different methods of temporary external hemostasis at the pre-hospital stage of emergency medical care, taking into account the localization of injuries. Material and Methods. The cases (n=86) of prehospital emergency medical care for patients with external bleeding were studied. The data on the victims (age, gender), clinical status (type and location of injuries, type of bleeding), the volume of emergency care, the hemostasis methods used to control bleeding, their efficiency, side effects, difficulty of use were collected, and the duration of the pre-hospital stage were also taken into account. Results. The study found out that the most common cause of external bleeding is domestic accident (45.35%) of cases. By the nature of tissue damage, the cut wounds prevailed. Multiple or combined injuries occurred in 13.95% of cases. A tourniquet was the main method of hemostasis. Contact hemostatic agents were used only in 2.32% of cases at the pre-hospital stage. In some cases, when attempting to control the external bleeding, several hemostatic agents were sequentially used due to the lack of their efficacy. In 17.4% of cases, the victims with multiple or combined injuries received intravenous administrationof the systemichemostatic agents. In the complex of anti-shock measures in patients with severe trauma 77.91% of the prehospital patients underwent infusion therapy. Isotonic crystalloid solutions were used. A reliable increase in the duration of the prehospital stage of victims who underwent to a combination of hemostasis methods and intravenous infusion has been registered. The increase in the length of the prehospital stay occurs at the expense of the time required for creation of venous access and initiate infusion. The authors propose the algorithm for temporal control of external bleeding during emergency medical care at the pre-hospital stage. Conclusion. The main principles that need to be guided in the choice of any methods for stopping of bleeding are the speed and reliability of hemostasis for entire time of evacuation and the minimum damaging effect on the tissues. What this paper adds The effectiveness of various methods for temporal external hemostasis at the pre-hospital stage of emergency medical treatment taking into account the localization of injuries was estimated. It has been established that the main principles that must be followed in choosing any of the methods for stopping the bleeding are the speed and reliability of hemostasis for the entire duration of evacuation and the minimal tissue damage.
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Lipatov, Vyacheslav Aleksandrovich, Sergej Viktorovich Lazarenko, Dmitry Andreevich Severinov, and Anastasia Valeryevna Tarasova. "STUDY OF HISTOLOGICAL FEATURES OF LIVER REACTION USING NEW SAMPLES OF POLYMERIC HEMOSTATIC MATERIALS IN THE EXPERIMENT IN VIVO." Journal of Experimental and Clinical Surgery 13, no. 1 (February 25, 2020): 34–40. http://dx.doi.org/10.18499/2070-478x-2020-13-1-34-40.
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Abdominal trauma in 26.7-40.8% of cases is accompanied by trauma of parenchymal organs. The condition of the patient with liver injury in 75% of cases is complicated by the development of hemorrhagic shock. In surgery, to achieve local hemostasis, the most promising method is the topical use of application hemostatic agents, while the composition of such funds determines the effectiveness of hemostasis.
The aim of the study is to assess the histological characteristics of the liver using new samples of polymeric hemostatic materials in the experiment in vivo.
Materials and methods. As research material was used hemostatic sponge based on natrocarbonatite (Na-CMC) (developed jointly with Linteks, Russia. Saint-Petersburg.) In the experimental groups of laboratory animals under general anesthesia produced a median laparotomy and simulated planar surface injury to the liver. Parenchymal bleeding was stopped by applying application hemostatic materials. An autopsy of the injured area of the liver was performed together with an implanted hemostatic agent. The thickness of the capsule, the size of the Central veins of the liver, the number of dual-core hepatocytes, the size of hepatocytes and their nuclei were measured.
Results. The indicator of the area of hepatocytes of groups No. 4 and No. 5 are as close as possible to group No. 1 indicators. Similar to the area of hepatocytes, difference of the area of their kernels in groups No. 6, No. 7 are statistically not significant in comparison with group No. 1. In groups No. 6 and No. 5 the area of kernels is authentically increased, but does not reach values of control group. The greatest statistically significant increase in thickness of the capsule in comparison with group No. 1, group No. 2 and No. 3 was observed in group No. 4. The area of the central vein is authentically increased in comparison with control group in groups No. 2, No. 3 and No. 6. The number of two-nuclear hepatocytes in comparison with norm, group No. 4 and group No. 1 is reliable above in groups No. 6 and No. 7.
Conclusion. Histologic reorganizations, changes of morphological structure in places of a trauma and implantation of the tested samples are less expressed when using haemo static implants of experimental groups No. 3-№6 (styptic local means on the basis of Na-KMTs with addition of aminocaproic, acetilsalicylic and aminoacetic acids).
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Sammartino, Gilberto, David M. Dohan Ehrenfest, Francesco Carile, Mariano Tia, and Paolo Bucci. "Prevention of Hemorrhagic Complications After Dental Extractions Into Open Heart Surgery Patients Under Anticoagulant Therapy: The Use of Leukocyte- and Platelet-Rich Fibrin." Journal of Oral Implantology 37, no. 6 (December 1, 2011): 681–90. http://dx.doi.org/10.1563/aaid-joi-d-11-00001.
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Leukocyte- and platelet-rich fibrin (L-PRF) is a biomaterial commonly used in periodontology and implant dentistry to improve healing and tissue regeneration, particularly as filling material in alveolar sockets to regenerate bone for optimal dental implant placement. The objective of this work was to evaluate the use of L-PRF as a safe filling and hemostatic material after dental extractions (or avulsions) for the prevention of hemorrhagic complications in heart surgery patients without modification of the anticoagulant oral therapy. Fifty heart surgery patients under oral anticoagulant therapy who needed dental extractions were selected for the study. Patients were treated with L-PRF clots placed into 168 postextraction sockets without modification of anticoagulant therapy (mean international normalized ratio = 3.16 ± 0.39). Only 2 patients reported hemorrhagic complications (4%), all of which resolved a few hours after the surgery by compression and hemostatic topical agents. Ten patients (20%) showed mild bleeding, which spontaneously resolved or was resolved by minimal compression less than 2 hours after surgery. No case of delayed bleeding was reported. The remaining 38 patients (76%) showed an adequate hemostasis after the dental extractions. In all cases, no alveolitis or painful events were reported, soft tissue healing was quick, and wound closure was always complete at the time of suture removal one week after surgery. The proposed protocol is a reliable therapeutic option to avoid significant bleeding after dental extractions without the suspension of the continuous oral anticoagulant therapy in heart surgery patients. Other applications of the hemostatic and healing properties of L-PRF should be investigated in oral implantology.
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Yoo, In Kyung, Keumyeon Kim, Gawon Song, Mi-Young Koh, Moon Sue Lee, Abdullah Özgür Yeniova, Haeshin Lee, and Joo Young Cho. "Endoscopic application of mussel-inspired phenolic chitosan as a hemostatic agent for gastrointestinal bleeding: A preclinical study in a heparinized pig model." PLOS ONE 16, no. 5 (May 14, 2021): e0251145. http://dx.doi.org/10.1371/journal.pone.0251145.
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Marine mussels secrete adhesive proteins to attach to solid surfaces. These proteins contain phenolic and basic amino acids exhibiting wet adhesion properties. This study used a mussel-inspired hemostatic polymer, chitosan-catechol, to treat gastrointestinal bleeding caused by endoscopic mucosal resection in a heparinized porcine model. We aimed to evaluate the hemostatic efficacy and short-term safety of this wet adhesive chitosan-catechol. We used 15 heparinized pigs. Four iatrogenic bleeding ulcers classified as Forrest Ib were created in each pig using an endoscopic mucosal resection method. One ulcer in each pig was untreated as a negative control (no-treatment group). The other three ulcers were treated with gauze (gauze group), argon plasma coagulation (APC group), and chitosan-catechol hemostatic agent (CHI-C group) each. The pigs were sacrificed on Days 1, 5, and 10, and histological examination was performed (n = 5 per day). Rapid hemostasis observed at 2 min after bleeding was 93.3% (14/15) in the CHI-C group, 6.7% (1/15) in the no-treatment group, 13.3% (2/15) in the gauze group, and 86.7% (13/15) in the APC group. No re-bleeding was observed in the CHI-C group during the entire study period. However, a few re-bleeding cases were observed on Day 1 in the no-treatment, gauze, and APC groups and on Day 5 in the gauze and APC groups. On histological analysis, the CHI-C group showed the best tissue healing among the four test groups. Considering the results, chitosan-catechol is an effective hemostatic material with reduced re-bleeding and improved healing.
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Zhang, Baode, Ali Nabipour Chakoli, Jin Mei He, Yu Dong Huang, and Andrey N. Aleshin. "Hemostatic Effect of Aminated Multiwalled Carbon Nanotubes/Oxidized Regenerated Cellulose Nanocomposites." Journal of Nanoscience and Nanotechnology 19, no. 11 (November 1, 2019): 7410–15. http://dx.doi.org/10.1166/jnn.2019.16617.
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We have investigated the covalent conjugation of aminated multiwalled carbon nanotubes (MWCNTNH2)s with Oxidized Regenerated Cellulose (ORC) in order to enhance the hemostatic effect. The MWCNT-NH2s were prepared by functionalization of pristine MWCNTs (pMWCNTs) using amine groups. Neat ORC gauze and MWCNT-NH2s were reacted using glutamic acid as cross linking bridge. We investigated an amination of pMWCNTs as well as the dispersion of MWCNT-NH2s in the ORC gauze as matrix and their interfacial interactions by SEM and FT-IR. The results revealed that relatively strong interaction exists between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results in the significant increment of water uptake of MWCNT-NH2s/ORC composites with increasing the concentration of MWCNT-NH2s in composite. The in-vitro procoagulation test shows that the MWCNT-NH2s/ORC gauzes have significant procoagulant activity. The hemostatic evaluation of MWCNT-NH2s/ORC composites on rabbits shows that the aminated MWCNTs increase the rate of blood stopping and hence they decrease the blood loosing from injured sites. Hemostatic evaluation indicates that the MWCNT-NH2s/ORC gauze has a valuable hemostatic performance. The products of platelets release reaction, activated platelets glycoprotein and activated clotting enzymes were increased simultaneously. The mechanism of the hemostasis for MWCNT-NH2s/ORC gauze is discussed.
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SUGITACHI, AKIO, MICHIO KATO, MANABU MASUZAWA, and AKIRA TERADA. "A New Hemostatic Material, G - T - XIII." ASAIO Transactions 32, no. 1 (July 1986): 609–10. http://dx.doi.org/10.1097/00002216-198609000-00050.
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SUGITACHI, AKIO, MICHIO KATO, MANABU MASUZAWA, and AKIRA TERADA. "A New Hemostatic Material, G - T - XIII." ASAIO Transactions 32, no. 1 (July 1986): 609–10. http://dx.doi.org/10.1097/00002480-198609000-00050.
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Soldatova, Darya S., Aleksandr I. Begin, and Tatyana A. Ishunina. "Efficacy of the New Hemostatic Composite Based on Sodium Gel Carboxymethylcellulose and Aminocaproic Acid: Experimental Application after Partial Splenectomy in Rats." Journal of Experimental and Clinical Surgery 13, no. 4 (December 13, 2020): 319–25. http://dx.doi.org/10.18499/2070-478x-2020-13-4-319-325.
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Backgraund. Spleen surgery is mainly aimed to achieve effective hemostasis. The increasing popularity of topical hemostatic drugs is limited by their common side effect associated with the development of adhesions.The aim of study was to develop a new hemostatic drug with anti-adhesion activity. To achieve this, the authors have investigated 6% sodium carboxymethylcellulose gel with the addition of 5% aminocaproic acid. Materials and methods. The efficacy of the sample was studied experimentally in laboratory animals (rats, n = 87). The time of bleeding arrest, the amount of blood loss, the severity of the adhesion process, morphological and histological changes in tissues in the area of material implantation were assessed.Results. The study results evidenced that the new hemostatic drug reduced bleeding time by 68% (222.27 sec) and blood loss by 69% (285.6 mg) (p 0.01) after partial splenectomy, and prevented the development of adhesive process compared to the results obtained in the animals of the control group. On the 3rd day of the experiment application of the sample demonstrated that adhesions were completely absent, the coefficient of the severity of the adhesive process was 0 points; on the 7th and 14th days it was minimal - 0.481 and 0.486 points, respectively (p 0.05). A morphological study showed that the use of the new composite reduced the white pulp immunological reactivity associated with postoperative inflammatory processes, which is manifested by smaller lymphoid follicles and lower CD4 + T-lymphocytes expression.Conclusions. Thus, 6% sodium carboxymethylcellulose gel with the addition of 5% aminocaproic acid is an effective combined hemostatic and anti-adhesion agent that can be effectively applied in spleen surgery.
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Schenk, Worthington G., William D. Spotnitz, Sandra G. Burks, Peter H. Lin, Ruth L. Bush, and Alan B. Lumsden. "Absorbable Cyanoacrylate as a Vascular Hemostatic Sealant: A Preliminary Trial." American Surgeon 71, no. 8 (August 2005): 658–61. http://dx.doi.org/10.1177/000313480507100808.
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No absorbable cyanoacrylate tissue adhesive for safe internal surgical use is available. This prospective multicenter preliminary study was designed to evaluate the safety and effectiveness of an investigational absorbable cyanoacrylate (IAC) as an adjunct to hemostasis in arteriovenous shunt (AVS) procedures for dialysis access. Consenting adults (10) underwent placement of expanded polytetrafluoroethylene (ePTFE) upper extremity vascular grafts for AVS access using continuous 5-0 or 6-0 polypropylene after heparinization (≥3,000 units IV). Arterial anastomoses were evaluated after sealing with IAC followed by 120 seconds of polymerization time. After vascular clamp removal, the mean time to hemostasis was 9.1 ± 28.8 seconds. Additionally, 90 per cent (9/10) and 100 per cent (10/10) achieved hemostasis by 1 and 5 minutes, respectively. No patients required further adjunctive hemostatic measures. Adverse event safety data analysis through 12 weeks revealed occlusion of graft or vessel in four patients and graft thrombosis in one patient, all thought unrelated to sealant use. Other unrelated adverse events (bleeding, death, deep venous thrombosis, edema, erythema, hematoma, infection, and rash) occurred in single patients. Thus, IAC could be a useful sealant for vascular procedures with a potentially satisfactory safety profile. Larger, randomized, multicenter, prospective trials to further evaluate the use of this material are indicated and appropriate.
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Yang, Chul Min, Jeehee Lee, Su Yeon Lee, Haeshin Lee, Kiramage Chathuranga, Jongsoo Lee, and Wonho Park. "Silk Fibroin/Tannin/ZnO Nanocomposite Hydrogel with Hemostatic Activities." Gels 8, no. 10 (October 12, 2022): 650. http://dx.doi.org/10.3390/gels8100650.
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The inevitable bleeding and infections caused by disasters and accidents are the main causes of death owing to extrinsic trauma. Hemostatic agents are often used to quickly suppress bleeding and infection, and they can solve this problem in a short time. Silk fibroin (SF) has poor processibility in water, owing to incomplete solubility therein. In this study, aiming to overcome this disadvantage, a modified silk fibroin (SF-BGE), easily soluble in water, was prepared by introducing butyl glycidyl ether (BGE) into its side chain. Subsequently, a small amount of tannic acid (TA) was introduced to prepare an SF-BGE /TA solution, and ZnO nanoparticles (NPs) were added to the solution to form the coordination bonds between the ZnO and TA, leading to an SF-based nanocomposite hydrogel. A structural characterization of the SF-BGE, SF-BGE/TA, SF-BGE/TA/ZnO, and the coordination bonds between ZnO/TA was observed by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and the phase change was observed by rheological measurements. The pore formation of the SF-BGE/TA/ZnO hydrogel and dispersibility of ZnO were verified through energy-dispersive X-ray spectroscopy (EDS) and scanning electron microscopy (SEM). The cytocompatible and hemostatic performances of the SF-BGE/TA/ZnO NPs composite hydrogels were evaluated, and the hydrogels showed superior hemostatic and cytocompatible activities. Therefore, the SF-based nanocomposite hydrogel is considered as a promising material for hemostasis.
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Barannikov, Sergey V., Evgeniy F. Cherednikov, Igor S. Yuzefovich, Ludmila E. Mehantjeva, Igor F. Filev, Yuri V. Maleev, Evgeniy S. Ovsyannikov, and Violetta A. Maslova. "A Study of the Influence of New Generation Granulated Sorbents on the Processes Regulating the Aggregate State of the Blood with the Use of Piezoelectric Thromboelastography." International Journal of Biomedicine 11, no. 3 (September 9, 2021): 286–90. http://dx.doi.org/10.21103/article11(3)_oa6.
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Background: Gastroduodenal bleeding (GDB) and the improvement of endoscopic hemostasis (EH) remain a priority in emergency surgery. This article presents the results of an experimental study of the effects of granular sorbents (Aseptisorb, Aseptisorb-A, Aseptisorb-D) on the system regulating the aggregate state of the blood using modern capabilities of piezoelectric thromboelastography (TEG). Methods and Results: The study involved 12 healthy volunteers (9/75% men and 3/25% women) aged between 18 and 58 years, with the average age of 34.0(26.0;44.0) years. For the study, the blood of healthy volunteers with normal indicators of the system regulating the aggregate state of the blood was used. In vitro experiments: Several tests were performed with the blood of each volunteer. In the first experiment (the control stage), the blood cuvette did not contain the test material. At the second stage of the experiment, the hemostatic properties of new generation granulated sorbents (Aseptisorb, Aseptisorb-A, and Aseptisorb-D) were studied. Experimental studies have shown that the use of granular sorbents Aseptisorb, Aseptisorb-A, and Aseptisorb-D in varying degrees affects the links of platelet and coagulation hemostasis, providing acceleration of thrombosis processes while increasing the maximum density of the clot. These effects determine the effectiveness of the clinical use of these sorbents to stop various types of bleeding. Conclusion: Experimental studies of the effect of granular sorbents on the system regulating the aggregate state of the blood using piezoelectric TEG have shown that the use of Aseptisorb, Aseptisorb-A, and Aseptisorb-D can significantly reduce the time of blood clotting and increase the maximum clot density, which determines the possibility of the use of these sorbents in endoscopic hemostatic treatment for GDB.
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Zhong, Qing-Kun, Ze-Yin Wu, Ya-Qi Qin, Zhang Hu, Si-Dong Li, Zi-Ming Yang, and Pu-Wang Li. "Preparation and Properties of Carboxymethyl Chitosan/Alginate/Tranexamic Acid Composite Films." Membranes 9, no. 1 (January 8, 2019): 11. http://dx.doi.org/10.3390/membranes9010011.
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In this study, the porous composite films of carboxymethyl chitosan/alginate/tranexamic acid were fabricated, with calcium chloride as the crosslinking agent and glycerin as a plasticizer. The composite films were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The properties of the composite films, including water absorption, air permeability, and cumulative release rate, were tested. In addition, their hemostatic performance was evaluated. The results showed that the appearance of the films with good adhesion was smooth and porous. FTIR showed that chemical crosslinking between carboxymethyl chitosan and sodium alginate was successful. The excellent cumulative release of tranexamic acid in the composite films (60–80%) gives the films a significant procoagulant effect. This has good prospects for the development of medical hemostasis materials.
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Tkachenko, Aleksandr Nikolaevich, Dmitriy Svyatoslavovich Savitskiy, and Aleksandr Anatolievich Khromov. "Hemostasis in Liver Injuries: An Experimental Study." Journal of Experimental and Clinical Surgery 14, no. 1 (January 12, 2021): 47–52. http://dx.doi.org/10.18499/2070-478x-2021-14-1-47-52.
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Introduction. Bleeding in liver injuries is observed during natural disasters, massive injuries, in military field conditions and under other circumstances. The vital need for surgical treatment in these cases is obvious. As a rule, time for assistance is limited, and operations are performed by surgeons who do not have sufficient experience. Intraoperative hemostasis methods are not always effective. The search for intraoperative hemostasis methods has not stopped for many decades. Experimental study of the effect of new agents that allow for reliable hemostasis in liver damage is relevant for medical science and practice.Materials and methods. The experimental study included 116 white Wistar rats of both sexes weighed 230 25 g and 30 Chinchilla rabbits of both sexes weighed 2.5-3.5 kg. The following parameters were identified in experiments in vivo: the duration of bleeding in simulated liver injury without the use of hemostatics and with the use of alufer and viscostat preparations. A morphological study of the regenerative process in the liver was carried out on the 1st - 14th day after the injury. The biopsy material was fixed in 10% formalin solution. Staining of histological sections was carried out with hematoxylin and eosin, as well as Prussian blue (according to Max Perls) to detect iron compounds in tissues.Results. It was revealed that the implementation of hemostasis with the use of the alufer preparation is accompanied by a decrease in circulatory disorders (edema, hemorrhages); characterized by a more pronounced formation of the connective tissue in the area of drug application, which is partially retained (taking into account the time interval of the study) at the site of application.
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Rizky Amalia Putri, Muhammad Noor Diansyah, Ami Ashariati Ashariati, Siprianus Ugroseno Yudho Bintoro, Putu Niken Ayu Amrita, Merlyna Savitri Savitri, and Pradana Zaky Romadhon. "Diathesis Hemorrhagic, Coagulation and Fibrinolytic System." Biomolecular and Health Science Journal 5, no. 1 (April 28, 2022): 54–61. http://dx.doi.org/10.20473/bhsj.v5i1.35280.
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Bleeding is one of the most common complaints when coming to the hospital which can be mild to life-threatening. The balance of the impaired hemostasis system allows for abnormal bleeding such as hemorrhagic diathesis. Balance between blood clotting and bleeding is always maintained in the body under normal physiology. The coagulation system stops existing bleeding with vasoconstricts of blood vessels and the formation of early platelet plugs, this blockage is strengthened by the presence of a cascade of coagulations to form stable and sturdy blockages. Once bleeding has stopped, the fibrinolytic pathway is initiated to dissolve the blood clot to restore normal blood flow. balance the coagulants, fibrinolytic and inhibitor systems, creating a perfect physiological balance. Hemostatic imbalance is a global problem that can lead to thrombosis or hemorrhage.
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Miebach, Lea, Broder Poschkamp, Julia van der Linde, and Sander Bekeschus. "Medical Gas Plasma—A Potent ROS-Generating Technology for Managing Intraoperative Bleeding Complications." Applied Sciences 12, no. 8 (April 9, 2022): 3800. http://dx.doi.org/10.3390/app12083800.
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Cold medical gas plasmas are under pre-clinical investigation concerning their hemostatic activity and could be applied for intra-operative bleeding control in the future. The technological leap innovation was their generation at body temperature, thereby causing no thermal harm to the tissue and ensuring tissue integrity. This directly contrasts with current techniques such as electrocautery, which induces hemostasis by carbonizing the tissue using a heated electrode. However, the necrotized tissue is prone to fall, raising the risk of post-operative complications such as secondary bleedings or infection. In recent years, various studies have reported on the ability of medical gas plasmas to induce blood coagulation, including several suggestions concerning their mode of action. As non-invasive and gentle hemostatic agents, medical gas plasmas could be particularly eligible for vulnerable tissues, e.g., colorectal surgery and neurosurgery. Further, their usage could be beneficial regarding the prevention of post-operative bleedings due to the absence or sloughing of eschar. However, no clinical trials or individual healing attempts for medical gas plasmas have been reported to pave the way for clinical approvement until now, despite promising results in experimental animal models. In this light, the present mini-review aims to emphasize the potential of medical gas plasmas to serve as a hemostatic agent in clinical procedures. Providing a detailed overview of the current state of knowledge, feasible application fields are discussed, and possible obstacles are addressed.
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Zhang, Dongying, Zhang Hu, Lingyu Zhang, Sitong Lu, Fengyan Liang, and Sidong Li. "Chitosan-Based Thermo-Sensitive Hydrogel Loading Oyster Peptides for Hemostasis Application." Materials 13, no. 21 (November 9, 2020): 5038. http://dx.doi.org/10.3390/ma13215038.
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Uncontrolled massive hemorrhage is one of the principal causes of death in trauma emergencies. By using catechol-modified chitosan (CS-C) as the matrix material and β glycerol phosphate (β-GP) as a thermo-sensitive agent, chitosan-based thermo-sensitive hydrogel loading oyster peptides (CS-C/OP/β-GP) were prepared at physiological temperature. The hemostatic performance of CS-C/OP/β-GP hydrogel was tested in vivo and in vitro, and its biological safety was evaluated. The results showed that the in vitro coagulation time and blood coagulation index of CS-C/OP/β-GP hydrogel were better than those of a commercial gelatin sponge. Notably, compared with the gelatin sponge, CS-C/OP/β-GP hydrogel showed that the platelet adhesion and erythrocyte adsorption rates were 38.98% and 95.87% higher, respectively. Additionally, the hemostasis time in mouse liver injury was shortened by 19.5%, and the mass of blood loss in the mouse tail amputation model was reduced by 18.9%. The safety evaluation results demonstrated that CS-C/OP/β-GP had no cytotoxicity to L929 cells, and the hemolysis rates were less than 5% within 1 mg/mL, suggesting good biocompatibility. In conclusion, our results indicate that CS-C/OP/β-GP is expected to be a promising dressing in the field of medical hemostasis.
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El Halawany, Mai, Randa Latif, and Mohamed H. H. AbouGhaly. "Hemostatic Alginate/Nano-Hydroxyapatite Composite Aerogel Loaded with Tranexamic Acid for the Potential Protection against Alveolar Osteitis." Pharmaceutics 14, no. 10 (October 21, 2022): 2255. http://dx.doi.org/10.3390/pharmaceutics14102255.
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Wound control in patients on anticoagulants is challenging and often leads to poor hemostasis. They have a higher tendency to develop alveolar osteitis after tooth extraction. The application of a hemostatic dressing that has a high absorbing capacity and is loaded with an antifibrinolytic drug could help in controlling the bleeding. Alginate/nano-hydroxyapatite (SA/Nano-HA) composite aerogels loaded with tranexamic acid (TXA) were prepared. Nano-HA served as a reinforcing material for the alginate matrix and a source of calcium ions that helps in blood clotting. It influenced the porosity and the water uptake capacity. TXA release from SA/Nano-HA aerogels showed a biphasic profile for up to 4 h. Blood coagulation studies were performed on human whole blood. The TXA-loaded aerogel significantly reduced the clotting time by 69% compared to the control (p < 0.0001). Recalcification time was significantly reduced by 80% (p < 0.0001). Scanning electron microscopy analysis revealed the porous nature of the aerogels and the ability of the optimum aerogel to activate and adhere platelets to its porous surface. The cell migration assay showed that there was a delay in wound healing caused by the TXA aerogel compared to the control sample after treating human fibroblasts. Results suggest that the developed aerogel is a promising dressing that will help in hemostasis after tooth extraction.