Journal articles: 'Hematoxylin'

1

Bosma, Rob B., and Ezzat A. Elias. "Environmentally Friendly Mercury-Free Hematoxylin." Journal of Histotechnology 16, no. 4 (December 1993): 371–74. http://dx.doi.org/10.1179/his.1993.16.4.371.

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2

Hales, Pat. "Hematoxylin Stain for Epon Sections." Microscopy Today 5, no. 4 (May 1997): 20. http://dx.doi.org/10.1017/s1551929500061423.

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3

Titford, M. "The long history of hematoxylin." Biotechnic & Histochemistry 80, no. 2 (January 2005): 73–78. http://dx.doi.org/10.1080/10520290500138372.

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4

Llewellyn, BD. "Nuclear staining with alum hematoxylin." Biotechnic & Histochemistry 84, no. 4 (January 2009): 159–77. http://dx.doi.org/10.1080/10520290903052899.

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5

Smith, A. A. "Bismuth hematoxylin staining of nucleic acids." Proceedings, annual meeting, Electron Microscopy Society of America 53 (August 13, 1995): 930–31. http://dx.doi.org/10.1017/s0424820100141020.

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Hematoxylin is readily oxidized to the blue pigment hematein. Hematein can complex with a wide variety of metal salts or mordants to form mordant dyes. These dyes stain various components of animal tissues. The component stained depends on the metal in the mordant dye.Alum hematoxylin uses trivalent aluminum ion as a mordant. At extreme dilution, requiring long staining times, alum hematoxylin is a specific stain for nucleic acids. As usually used, alum hematoxylin stains nucleoproteins, staining them nearly as well after the removal of the nucleic acids as before.Bismuth shows a high affinity for nucleic acids. Although all common bismuth salts are insoluble in water, sodium bismuthate reacts with an aqueous solution of hematoxylin to produce hematein and trivalent bismuth ions. The bismuth ions complex with the hematein to form a water-soluble “bismuth hematoxylin.” HEPES (N-[2-hydroxyethyl]piperazine- N'-2-ethanesulfonic acid), 8 mg/ml of solution, greatly increases the amount of bismuth hematoxylin formed. Glycerol, 1/8 v/v, improves the stability of the complex.

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6

Garvey, Winsome. "Modification of the Mayer Hematoxylin Stain." Journal of Histotechnology 14, no. 3 (September 1991): 163–65. http://dx.doi.org/10.1179/his.1991.14.3.163.

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7

Graham, Effin T. "A Quick-Mixed Aluminum Hematoxylin Stain." Biotechnic & Histochemistry 66, no. 6 (January 1991): 279–81. http://dx.doi.org/10.3109/10520299109109988.

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8

Roach, John B., and Allen A. Smith. "Bismuth Hematoxylin Stain for Arginine Residues." Biotechnic & Histochemistry 72, no. 1 (January 1997): 49–54. http://dx.doi.org/10.3109/10520299709082212.

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9

Fujii, Mutue T., and Marcelo Guerra. "Improved Hematoxylin Staining for Algal Cytogenetics." Biotechnic & Histochemistry 73, no. 2 (January 1998): 78–81. http://dx.doi.org/10.3109/10520299809140510.

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10

Hochstim, Christian J., Judy Yujin Choi, Derek Lowe, Rizwan Masood, and Dale H. Rice. "Biofilm Detection With Hematoxylin-Eosin Staining." Archives of Otolaryngology–Head & Neck Surgery 136, no. 5 (May 17, 2010): 453. http://dx.doi.org/10.1001/archoto.2010.62.

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11

Ferreira, Claudio Santos. "Staining of intestinal protozoa with heidenhain's iron Hematoxylin." Revista do Instituto de Medicina Tropical de São Paulo 45, no. 1 (January 2003): 43–44. http://dx.doi.org/10.1590/s0036-46652003000100009.

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Due to its unique properties, iron hematoxylin has been traditionally recommended for staining intestinal protozoa. This process can be simplified by reducing the number of steps and periods of permanence of the slides in some of the liquids used, without detriment to the quality of the results. Thus iron hematoxylin becomes adequate for routine use. Hematoxylin is a natural dye extracted from Haematoxylon campechianum, of the family Leguminosae. It must first be 'ripened', i.e. oxidized to hematein, which reacts with ferric ammonium sulphate to produce the ferric lake (iron hematoxylin), a basic dye. Iron hematoxylin most frequently stains regressively, i.e. the slides are first overstained and then differentiated.

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12

Ramaswamy, Anikode Subramanian, and Panati Dayasagar. "A Study of Xylene Free Hematoxylin and Eosin Staining Procedure." Annals of Advance Medical Sciecnes 1, no. 1 (December 9, 2017): A16—A21. http://dx.doi.org/10.21276/aams.1772.

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13

Maxson, Julia E. "A new role for hematoxylin: targeting CALR." Blood 137, no. 14 (April 8, 2021): 1848–49. http://dx.doi.org/10.1182/blood.2020009852.

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14

Gallagher, Jennifer A., Verna J. Russell, and Gregory M. Becker. "Hematoxylin and Lee's Methylene Blue-Basic Fuchsin." Laboratory Medicine 16, no. 9 (September 1, 1985): 538–40. http://dx.doi.org/10.1093/labmed/16.9.538.

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15

Davis, Daniel A. "Optimize Oxidation for the Fastest Hematoxylin Staining." Dermatologic Surgery 38, no. 8 (August 2012): 1331–35. http://dx.doi.org/10.1111/j.1524-4725.2012.02428.x.

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16

Ferrer-Roca, Olga, José A. Pérez Gómez, and Maritza Estévez. "Chromatin Texture from Hematoxylin Stained Thyroid Lesions." Analytical Cellular Pathology 17, no. 4 (1998): 209–17. http://dx.doi.org/10.1155/1998/320465.

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Quantitative aspects of cytology and histology should be considered in diagnostic standardisation processes. The present paper summarises the cytological differences detected in 75 thyroid lesions using a computerized textural analysis.Cells stained with progressive hematoxylin and taken from paraffin blocks were overlaid with the extracted texture. This technique was based on the lineal detection of a grey level gradient of the common logarithm of the integrated optical density (IOD) of each nucleus.Diffuse and nodular goiters (36 cases) were demonstrated to be composed of small cells containing high density texture that, on microscopical visual inspection, gave a “salt and pepper” appearance. The adenomatous goiters (2 cases) and adenomas (26 cases) were composed of low texture cells with a visual “blurry or smudgy” chromatin, while the atypical adenomas with capsular invasion (4 cases) were characterised by a “woodworm” nuclear appearance that produced the highest texture of the series. Finally, encapsulated folliculo-papillary carcinomas (3 cases) were composed of large clear nuclei with high IOD, low texture, and scattered lines that resulted in an “empty grape skin” aspect.Our findings seam to confirm the suitability of computerized textural techniques that aid in recognizing cell microscopic features objectively. The one used in the present work, based on a mathematical function of the DNA content of each individual nucleus (IOD), fulfills all microscopy detection criteria.

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17

Smith, Alien A. "A Vanadate Hematoxylin Stain for Basic Proteins." Biotechnic & Histochemistry 70, no. 5 (January 1995): 258–62. http://dx.doi.org/10.3109/10520299509108203.

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18

Budantsev, A. Yu, and O. A. Rudneva. "A Simple Method to Determine Hematoxylin Ripening." Biotechnic & Histochemistry 74, no. 4 (January 1999): 181–84. http://dx.doi.org/10.3109/10520299909047972.

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19

Ali, Faisal R., Guy E. Orchard, and Rajeev Mallipeddi. "Hematoxylin in History—The Heritage of Histology." JAMA Dermatology 153, no. 3 (March 1, 2017): 328. http://dx.doi.org/10.1001/jamadermatol.2016.0506.

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20

Sokolová, Romana, Ilaria Degano, Magdaléna Hromadová, Jana Bulíčková, Miroslav Gál, and Michal Valášek. "Oxidation pathways of natural dye hematoxylin in aqueous solution." Collection of Czechoslovak Chemical Communications 75, no. 11 (2010): 1097–114. http://dx.doi.org/10.1135/cccc2010096.

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The oxidation mechanism of hematoxylin was studied in phosphate buffers and 0.1 M KCl by cyclic voltammetry and UV-Vis spectroscopy under deaerated conditions. The redox potential of hematoxylin in buffered solution strongly depends on pH. A two electron oxidation is preceded by deprotonation. The homogeneous rate of deprotonation process of hematoxylin in 0.1 M phosphate buffer is kd = (2.5 ± 0.1) × 104 s–1. The cyclic voltammetry under unbuffered conditions shows the distribution of various dissociation forms of hematoxylin. The dissociation constants pK1 = 4.7 ± 0.2 and pK2 = 9.6 ± 0.1 were determined using UV-Vis spectroscopy. The final oxidation product was identified by gas chromatography with mass spectrometry detection as hemathein. The distribution of oxidation products differs under buffered and unbuffered conditions. The dye degradation in natural unbuffered environment yields hemathein and hydroxyhematoxylin, which is absent in buffered solution.

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21

Dapson, R., RW Horobin, and J. Kiernan. "Hematoxylin shortages: their causes and duration, and other dyes that can replace hemalum in routine hematoxylin and eosin staining." Biotechnic & Histochemistry 85, no. 1 (January 2010): 55–63. http://dx.doi.org/10.3109/10520290903048400.

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22

Bennet, R. J. "The response of lucerne and red clover roots to aluminium/hematoxylin: how universal is the hematoxylin test for aluminium?" South African Journal of Plant and Soil 14, no. 3 (January 1997): 120–26. http://dx.doi.org/10.1080/02571862.1997.10635093.

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23

Bogdanov, L. A., and A. G. Kutikhin. "Optimization of hematoxylin and eosin staining of heart, blood vessels, liver, and spleen." Fundamental and Clinical Medicine 4, no. 4 (December 28, 2019): 70–77. http://dx.doi.org/10.23946/2500-0764-2019-4-4-70-77.

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Aim. To optimize hematoxylin and eosin staining protocol for heart, blood vessels, liver, and spleen.Methods. Heart (ventricles), abdominal aorta, liver (right lobe), and spleen (left part) of the Wistar rats were excised, fixed in 10% neutral phosphate buffered formalin for 24 h, washed in tap water for 2 h, dehydrated in ascending ethanol series (70%, 80%, and 95%) and isopropanol, embedded into paraffin and then sectioned (5 μm) using rotary microtome. For regressive staining, incubation time in Harris hematoxylin was 5 or 10 minutes, time of exposure to differentiation alcoholicaqueous eosin was 1 or 2 minutes. For progressive staining, incubation time in Carazzi’s hematoxylin and eosin was the same but the differentiation solution was not utilized.Results. Progressive staining retained tissue integrity and accelerated staining protocol as compared to regressive staining due to absence of exposure to aggressive acid alcohol differentiation solution. The optimized protocol for heart, aorta and liver, similar for regressive and progressive staining, included incubation in hematoxylin for 10 minutes and eosin for 2 minutes. Time of exposure to differentiation solution (2 or 10 seconds) was defined by the desirable shade. For spleen, however, the optimized protocol included staining in hematoxylin for 5 minutes and eosin for 2 minutes, with 10 seconds in differentiation solution for regressive staining.Conclusion. Progressive hematoxylin is preferable over regressive hematoxylin for staining of heart, aorta, liver, and spleen.

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24

Gharravi, A. M., M. J. Golalipour, R. Ghorbani, and M. Khazaei. "Effects Modification of Iron Hematoxylin on Neuron Staining." Pakistan Journal of Biological Sciences 10, no. 5 (February 15, 2007): 768–72. http://dx.doi.org/10.3923/pjbs.2007.768.772.

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25

Smith, Allen A. "Zirconyl Hematoxylin: a New Stain for Acidic Mucins." Biotechnic & Histochemistry 75, no. 3 (January 2000): 124–31. http://dx.doi.org/10.3109/10520290009066490.

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26

Stancu, Adrian, Alina Ghise, Marius Pentea, Dana Emilia Velimirovici, Sorin Pasca, Liliana Carpinisan, and Romeo Teodor Cristina. "Hematoxylin - eosin- Staining in a Dog Polyarteritys Nodosa." Materiale Plastice 54, no. 2 (June 30, 2017): 302–3. http://dx.doi.org/10.37358/mp.17.2.4838.

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Polyartetrtys nodosa (PAN) was diagnosed histologically in a dog 14 months old, that presented in the necropsy a hemorrhagic diathesis. The specific lesions were present in the muscular arteries of middle and small caliber and in the arterioles from heart. The modifications are segmentail, chronic, evolving from fibrinoid necrosis to the polyphasic, transmural, neutrophilic, leucocytodastic, macrophagic, lympho-plasmodtic and finally fibrotic vasculitis. The elastolisis predisposes to micro-aneurisms and micro-hemorrhages. Unlikethe perivascularitis, the inflammatory processis extended to the surrounding tissues.

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27

Greenberg, Stephen R. "A Light Resistant, Permanent Eosin Counterstain For Hematoxylin." Journal of Histotechnology 10, no. 1 (March 1987): 41. http://dx.doi.org/10.1179/his.1987.10.1.41.

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28

Takahashi, Masae, and Tadashi Tezuka. "Hematoxylin Stainable Epidermal Protein of the Newborn Rat." Journal of Dermatology 16, no. 3 (June 1989): 178–83. http://dx.doi.org/10.1111/j.1346-8138.1989.tb01245.x.

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29

Tran, David, Martin Golick, Harold Rabinovitz, Daniel Rivlin, George Elgart, and Barbara Nordlow. "Hematoxylin and Safranin O Staining of Frozen Sections." Dermatologic Surgery 26, no. 3 (March 2000): 197–99. http://dx.doi.org/10.1046/j.1524-4725.2000.09220.x.

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30

FIES, M., and K. FRIEDRICH. "ChemInform Abstract: Carbon Analogues of Brasilin and Hematoxylin." ChemInform 26, no. 25 (August 17, 2010): no. http://dx.doi.org/10.1002/chin.199525190.

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31

Xu, Min, Karen M. Chisholm, Guang Fan, Anne M. Stevens, and Joe C. Rutledge. "Hematoxylin Bodies in Pediatric Bone Marrow Aspirates and their Utility in the Diagnosis of Systemic Lupus Erythematosus." Pediatric and Developmental Pathology 21, no. 3 (October 9, 2017): 300–307. http://dx.doi.org/10.1177/1093526617734948.

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In our recent case report, the finding of lupus erythematosus (LE) cells in a bone marrow aspirate led to the diagnosis of systemic lupus erythematosus (SLE) and appropriate treatment, although the patient was not clinically suspected to have SLE. To determine whether LE cells are present in the bone marrow aspirates of SLE patients, but overlooked in routine bone marrow morphology review, bone marrow aspirates from 30 pediatric patients (15 with SLE and 15 with other diagnoses) evaluated by rheumatologists were reviewed. LE cells were found in the bone marrow aspirates of only 1 SLE patient and none in non-SLE patients. However, hematoxylin bodies were identified in 53% (8/15) of SLE patients. Neither hematoxylin bodies nor LE cells were found in the aspirates from patients with other disorders. Three additional pediatric patients identified prospectively were found to have hematoxylin bodies in the bone marrow aspirates. Although the diagnosis was not initially suspected, 2 of the 3 patients were subsequently diagnosed with SLE. All patients with hematoxylin bodies and SLE had antinuclear antibody titers ≥1:640 with a homogeneous staining pattern. In addition, bone marrow aspirates of 9 adult patients were reviewed, and neither LE cells nor hematoxylin bodies were identified. In summary, hematoxylin bodies were present in the bone marrow aspirates of many pediatric SLE patients, while LE cells were rare. The finding of hematoxylin bodies in pediatric bone marrow aspirates is a helpful and specific diagnostic clue that may lead to the diagnosis of SLE when other clinical features are nonspecific.

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32

Yilmaz, Mehmet, Adem Kocyigit, Burcu Bozkurt Cirak, Hatice Kacus, Umit Incekara, and Sakir Aydogan. "The comparison of Co/hematoxylin/n-Si and Co/hematoxylin/p-Si devices as rectifier for a wide range temperature." Materials Science in Semiconductor Processing 113 (July 2020): 105039. http://dx.doi.org/10.1016/j.mssp.2020.105039.

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33

Ellyawati, Ellyawati. "PENENTUAN WAKTU YANG TEPAT PADA PROSES STAINING DALAM PEMBUATAN PREPARAT HISTOLOGIS HATI." Jurnal TEMAPELA 1, no. 1 (July 26, 2018): 28–30. http://dx.doi.org/10.25077/temapela.1.1.28-30.2018.

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Hematoxylin-Eosin merupakan salah satu jenis pewarnaan jaringan umum digunakan dalam pewarnaan jaringan seperti dalam pewarnaan jaringan hati. penelitian bertujuan untuk mengetahui waktu yang tepat dalam pembuatan preparat histologi hati dan memudahkan mahasiswa-mahasiswa praktikum dan penelitian mengenai histologi hati dalam menggunakan waktu pada proses staining Hematoxylin-Eosin. Penelitian ini dilaksanakan pada bulan November 2016 di Laboratorium Struktur Perkembangan Hewan, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Padang. Pengamatan dilakukan dengan membandingkan preparat jaringan hati yang diwarnai menggunakan Hematoxylin-Eosin stain dengan tiga jenis waktu yang berbeda. Hasil penelitian menunjukkan bahwa penggunaan Hematoxylin selama 20 detik dan Eosin selama 20 detik memberikan hasil yang paling baik dibandingkan lama waktu lainnya sehingga didapatkan hasil preparat yang lebih baik.

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34

Ortiz-Hidalgo, Carlos, and Sergio Pina-Oviedo. "Hematoxylin: Mesoamerica’s Gift to Histopathology. Palo de Campeche (Logwood Tree), Pirates’ Most Desired Treasure, and Irreplaceable Tissue Stain." International Journal of Surgical Pathology 27, no. 1 (July 13, 2018): 4–14. http://dx.doi.org/10.1177/1066896918787652.

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Hematoxylin is a basic dye derived from the heartwood of Palo de Campeche ( Haematoxylum campechianum), the logwood tree native to Mexico and Central America. Haematoxylum means “bloodwood” in reference to its dark-red heartwood and campechianum refers to its site of origin, the coastal city of Campeche on the Yucatan Peninsula, Mexico. Hematoxylin is colorless but it turns into the color dye hematein after oxidation (ripening). The dyeing property of logwood was well-known to the natives of the Yucatan Peninsula before the arrival of the Spaniards who brought it to Europe shortly after the discovery of the Americas. An important trade soon developed related to growing and preparing hematoxylin for dyeing fabrics. Pirates discovered that one shipload of logwood was equivalent to a year’s value from any other cargo, and by 1563, more than 400 pirate vessels wandered the Atlantic Ocean and attacked Spanish galleons transporting gold, silver, and logwood from the Americas to Europe. Hematoxylin and eosin is a staining method that dates back to the late 19th century. In 1865 and 1891, Böhmer and Meyer, respectively, first used hematoxylin in combination with a mordant (alum). Later, with the use of anilines by Ehrlich, the repertoire of stains expanded rapidly resulting in the microscopic descriptions of multiple diseases that were defined by their stainable features. Today hematoxylin, along with eosin, remains the most popular stain in histology.

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35

Chen, Jia-Mei, Yan Li, Jun Xu, Lei Gong, Lin-Wei Wang, Wen-Lou Liu, and Juan Liu. "Computer-aided prognosis on breast cancer with hematoxylin and eosin histopathology images: A review." Tumor Biology 39, no. 3 (March 2017): 101042831769455. http://dx.doi.org/10.1177/1010428317694550.

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With the advance of digital pathology, image analysis has begun to show its advantages in information analysis of hematoxylin and eosin histopathology images. Generally, histological features in hematoxylin and eosin images are measured to evaluate tumor grade and prognosis for breast cancer. This review summarized recent works in image analysis of hematoxylin and eosin histopathology images for breast cancer prognosis. First, prognostic factors for breast cancer based on hematoxylin and eosin histopathology images were summarized. Then, usual procedures of image analysis for breast cancer prognosis were systematically reviewed, including image acquisition, image preprocessing, image detection and segmentation, and feature extraction. Finally, the prognostic value of image features and image feature–based prognostic models was evaluated. Moreover, we discussed the issues of current analysis, and some directions for future research.

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36

Rahmawati, Tri, Yadi Apriyadi, and Mamay. "UTILIZATION OF 1% OF METHYLENE BLUE IN STAINING HISTOPATHOLOGICAL PREPARATIONS AT ANATOMIC PATHOLOGY LABORATORY." Indonesian Journal of Medical Laboratory Science and Technology 2, no. 2 (August 27, 2020): 93–100. http://dx.doi.org/10.33086/ijmlst.v2i2.1563.

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Tissue staining using hematoxylin-eosin (HE) is a standard method of histopathological staining. The tissue staining is hampered when there is no hematoxylin reagent in laboratory. Therefore, other reagents are needed that can replace the use of hematoxylin. Methylene blue is a basic dyes that interact with cell nuclei which has a negative ionic charge of the tissue. It can be used as an alternative nuclei staining. This study aims to evaluate the use of 1% of methylene blue in cell nuclei staining in histopathological preparations. The research sample were 15 pathology preparations which were randomly selected including breast cancer, cervical cancer and ovarian cancer in the bank of sampel at anatomical pathology laboratory of RSUD Dr. Slamet Garut, Indonesia. The experiment showed that the methylene blue dyes yielded “worth” result (40%) and “poorly” result (60%). Further research can be carried out by modifying the pH of 1% of methylene blue reagent so that it can maximize the staining preparations results as good as those using hematoxylin.

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37

OHHASHI, Minoru, Takayuki SUZUKI, Atsushi IMAI, and Kumiko OGAWA. "Simple, reliable cell block preparation using hematoxylin S solution." Journal of the Japanese Society of Clinical Cytology 48, no. 4 (2009): 159–65. http://dx.doi.org/10.5795/jjscc.48.159.

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38

Guerra, Marcelo. "Hematoxylin: a simple, multiple-use dye for chromosome analysis." Genetics and Molecular Biology 22, no. 1 (March 1999): 77–80. http://dx.doi.org/10.1590/s1415-47571999000100016.

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A staining mixture of hematoxylin-iron alum combined with a strong hydrochloric hydrolysis was successfully applied for chromosome observation of several kinds of plants and some animals. Slightly different procedures were developed for different materials and objectives. For plant cells, the most important technical aspect was the use of 5 N HCl hydrolysis, which resulted in a very transparent cytoplasm, combined with an intense, specific hematoxylin stain. This technique is recommended for cytogenetical analysis in general, and it is especially indicated for practical classes, due to its simplicity and high reproducibility of results. Moreover, the deep contrast observed makes this technique very useful for sequential staining of cells previously analyzed with other stains, as well as for materials with fixation problems.

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39

Kiernan, JA, and RW Horobin. "A special issue devoted to hematoxylin, hematein, and hemalum." Biotechnic & Histochemistry 85, no. 1 (January 2010): 5–6. http://dx.doi.org/10.3109/10520290903048368.

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40

Elston, Dirk M. "Medical Pearl: Fluorescence microscopy of hematoxylin-eosin-stained sections." Journal of the American Academy of Dermatology 47, no. 5 (November 2002): 777–79. http://dx.doi.org/10.1067/mjd.2002.120623.

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41

Shapiro, Stanley H., and Muzaffar M. Akram. "Microwave Iron Hematoxylin Stain: A Rapid Procedure for Histology." Laboratory Medicine 25, no. 12 (December 1, 1994): 795–98. http://dx.doi.org/10.1093/labmed/25.12.795.

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42

Benard, Solomon A. "Iron-Roselle: A Progressive Nuclear Stain Substitute for Hematoxylin." Journal of Histotechnology 31, no. 2 (June 2008): 57–59. http://dx.doi.org/10.1179/his.2008.31.2.57.

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43

Roubani-Kalantzopoulou, Fani, and Nicholas A. Katsanos. "The Reaction of Hematoxylin with Zirconyl Chloride Studied Spectrophotometrically." Zeitschrift für Physikalische Chemie 149, no. 2 (January 1986): 165–81. http://dx.doi.org/10.1524/zpch.1986.149.2.165.

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44

Norton, Scott A. "The useful plants of dermatology. II. Haematoxylum and hematoxylin." Journal of the American Academy of Dermatology 34, no. 1 (January 1996): 149–51. http://dx.doi.org/10.1016/s0190-9622(96)90867-1.

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45

Smith, A. A. "Specific staining of tissue components with metal–hematoxylin complexes." Micron 33, no. 1 (January 2002): 95–103. http://dx.doi.org/10.1016/s0968-4328(00)00059-7.

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46

Brooks, Amos A. "Immunohistochemistry and Hematoxylin and Eosin on the Same Slide." Microscopy Today 8, no. 6 (August 2000): 51. http://dx.doi.org/10.1017/s1551929500052901.

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Small tissues are always difficult to work with. The limited size often leads to many technical problems. In spite of the care normally given to a specimen, the unthinkable can happen. A block may be cut through, slides may be broken, and sections may fall off. When this type of tragedy occurs, the creativity and technical abilities of a histology technician are often tested.An example of this occurred when a technician was cutting a prostate biopsy. Two slides were taken, with two sections on each. The slides were taken, with two sections on each. The slides were stained with hematoxylin and eosin (H&E), and coverslipped with a Tissue Tek automatic coverslipper. This coverslipper uses a plastic film, which causes the film to adhere to the slide with xylene. The slides were then given to the pathologist who then requested an immunohistochemical (IHC) test to be performed. In the process, the specimen was exhausted.

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47

Bettinger, Ch, and H. W. Zimmermann. "New investigations on hematoxylin, hematein, and hematein-aluminium complexes." Histochemistry 95, no. 3 (May 1991): 279–88. http://dx.doi.org/10.1007/bf00745000.

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48

Bettinger, Ch, and H. W. Zimmermann. "New investigations on hematoxylin, hematein, and hematein-aluminium complexes." Histochemistry 95, no. 3 (January 1991): 279–88. http://dx.doi.org/10.1007/bf00266778.

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49

Bettinger, Ch, and H. W. Zimmermann. "New investigations on hematoxylin, hematein, and hematein-aluminium complexes." Histochemistry 96, no. 3 (July 1991): 215–28. http://dx.doi.org/10.1007/bf00271540.

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50

Herath, Ajith, Namal Priyantha, Gamini Rajapakse, Veranja Karunaratne, and Anura Wickramasinghe. "Porphyrin-sensitized photo-oxidation of hematoxylin in oxygenated solutions." Journal of the National Science Foundation of Sri Lanka 35, no. 4 (December 28, 2007): 239. http://dx.doi.org/10.4038/jnsfsr.v35i4.1312.

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Journal articles on the topic 'Hematoxylin'

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