Journal articles: 'HeLa cell line'

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Beard, Jonathan. "A cell line called HeLa." New Scientist 205, no. 2748 (February 2010): 47. http://dx.doi.org/10.1016/s0262-4079(10)60428-9.

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Callaway, Ewen. "Deal done over HeLa cell line." Nature 500, no. 7461 (August 2013): 132–33. http://dx.doi.org/10.1038/500132a.

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Mandia, Sayati. "Cytotoxicity activity of Liverwort (Marchantia Polymorpha L.)Methanolic Extract to HeLa cell Line." Asian Pacific Journal of Health Sciences 7, no. 1 (March 30, 2020): 69–73. http://dx.doi.org/10.21276/apjhs.2020.7.1.13.

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Ekowati, Heny, Indwiani Astuti, and Mustofa Mustofa. "ANTICANCER ACTIVITY OF CALANONE ON HeLa CELL LINE." Indonesian Journal of Chemistry 10, no. 2 (July 21, 2010): 240–44. http://dx.doi.org/10.22146/ijc.21467.

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Calanone (coumarin derivate compound), isolated from Calophyllum sp. had been shown to have cytotoxic activity on leukemia L1210 cell line with IC50 = 59.40 mg/mL. Calanone presumed have anticancer activity on HeLa cervical carcinoma cell. This study was conducted to investigate the cytotoxic and apoptotic activity of calanone and its effect to p53 and p21 expression on HeLa cervical carcinoma cell. Cytotoxic assay of calanone was performed on HeLa cell line, using MTT assay. Apoptotic assay was performed on HeLa cell line incubated with calanone for 24 h, by immunofluororescence method, using fluorochromes ethidium bromide and acridine orange. Expression of p53 was examined on HeLa cell line, by PCR with p53 wild-type primer. Expression of p21 was examined on HeLa cell line, by immunohistochemistry method. 5-fluorourasil was used as positive control in cytotoxic, apoptotic assay, and p53 expression. The result showed that calanone has cytotoxic activity on HeLa cell line, with IC50 = 22.887 mg/mL, caused cytotoxicity through apoptotic mechanism, increase p53 tumor suppressor gene expression, while the p21 expression test showed a negative result. Keywords: Calanone, cytotoxic, HeLa cell line

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Krizman, D. B., S. Pathak, and R. Cailleau. "Double minutes in the HeLa cell line." Cancer Genetics and Cytogenetics 18, no. 1 (September 1985): 43–47. http://dx.doi.org/10.1016/0165-4608(85)90038-x.

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Fountoulakis, Michael, George Tsangaris, Ji-eun Oh, Antony Maris, and Gert Lubec. "Protein profile of the HeLa cell line." Journal of Chromatography A 1038, no. 1-2 (June 2004): 247–65. http://dx.doi.org/10.1016/j.chroma.2004.03.032.

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Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins. "Henrietta Lacks, HeLa Cells, and Cell Culture Contamination." Archives of Pathology & Laboratory Medicine 133, no. 9 (September 1, 2009): 1463–67. http://dx.doi.org/10.5858/133.9.1463.

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Abstract Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer cells, now called HeLa cells, grew rapidly in cell culture and became the first human cell line. HeLa cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa cells contaminated and overgrew other cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa cells. We describe the history behind the development of HeLa cells, including the first published description of Ms Lacks' autopsy, and the cell culture contamination that resulted. The debate over cell culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of the immortal HeLa cell line.

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Haridas, Renjini. "IN-VITRO CYTOTOXICITY ACTIVITY OF MALAXIS RHEEDEI SW METHANOL EXTRACT AGAINST HELA CELL LINE AND MCF- 7 CELL LINE." Asian Journal of Pharmaceutical and Clinical Research 9, no. 6 (November 1, 2016): 244. http://dx.doi.org/10.22159/ajpcr.2016.v9i6.14298.

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Cancer is a group of diseases caused by loss of cell cycle control. Cancer is associated with abnormal uncontrolled cell growth. The study was aimed to evaluation of the anticancer activity of the Malaxis rheedei Sw. on the HeLa cell line and MCF- 7cell line. The whole plant part of the Malaxis rheedei methanolic extract were tested for its inhibitory effect on HeLa Cell Line and MCF- 7cell line. The cytotoxicity of Malaxis rheedei on HeLa cell and MCF- 7cell line were evaluated by the MTT assay. Malaxis rheedei methanolic extract has significant cytotoxicity effect on MCF- 7cell line in concentration range between 18.75 to 300 µg/ml by using MTT assay and study also showed that inhibitory action on HeLa cell line inconcentration range between 18.75 to 300 µg/ml by using MTT assay. Methanol extract of the whole plant part of Malaxis rheedei was found to be 7.3%, 16.6%, 25.4%, 36.3% and 47.1%toxic in HeLa cell line and 7.9%, 13.9%, 26%, 48.4% and 66.3% toxic in MCF- 7cell line. IC50 value of Malaxis rheedei on MCF- 7cell was 167.76 µg/ml and IC50 value of Malaxis rheedei on HeLa Cell was not found by MTT assay. From the performed assay, methanol extract of these drug shows greater activity on MCF- 7cell line and little activity on HeLa cell line and that mean Malaxis rheedei can be used as anticancer activity.Keywords: Cytotoxicity Activity, MTT Assay, Malaxis rheedei Sw. , HeLa CellLine, MCF- 7Cell Line.

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Wang, Ching-Chiung, Lih-Geeng Chen, and Ling-Ling Yang. "Camelliin B induced apoptosis in HeLa cell line." Toxicology 168, no. 3 (November 2001): 231–40. http://dx.doi.org/10.1016/s0300-483x(01)00452-8.

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Fakhar-e-Alam, M., S. Kishwar, Y. Khan, M. Siddique, M. Atif, O. Nur, and M. Willander. "Tumoricidal effects of nanomaterials in HeLa cell line." Laser Physics 21, no. 11 (September 2, 2011): 1978–88. http://dx.doi.org/10.1134/s1054660x1119011x.

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Shokrzadeh, Mohammad, Zahra Eshghi, Abbas Ali Dehpouri, Rouya Ebrahimi, and Nasrin Ghassemi Barghi. "Cytotoxic Effects of Fe2O3 Nanoparticle on Cervical Cancer Cell Line (Hela) and Hepatocellular Carcinoma Cell Line (HepG2)." Advanced Science, Engineering and Medicine 12, no. 5 (May 1, 2020): 652–56. http://dx.doi.org/10.1166/asem.2020.2569.

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Nano forms of drugs are used to cure cancer not only due to selective cytotoxicity on cancer cells, but also because of the nontoxicity on normal cells. Nanoparticles of iron oxide have been widely surveyed for medical-health functions as a result of their high biocompatibility and simple synthesis. For this purpose this study was designed to evaluate the cytotoxic effects of iron oxide nanoparticle on cervical cancer cell line (Hela) and hepatocellular carcinoma cell line (HepG2) in vitro. Five concentrations of iron oxide nanoparticle (200, 400, 600, 800 and 1000 ppm) on Hela cell line and five concentrations (1, 50, 100, 500, 1000 ppm) on HepG2 cell line were prepared and tested with three replicates. The optical density of cell growth was analyzed by MTT assay. Flow cytometry used in order to evaluate apoptosis. The results showed that iron oxide nanoparticle at a concentration of 200 ppm significantly reduced cell growth on Hela compared to the control group after 48 hours. Although it reduced cell growth on the HepG2, and this reduction was time and dose dependent, it was not statistically significant. Iron oxide nanoparticle showed the effective cytotoxic on Hela cell line and ideally induced apoptosis to a high degree in these cells and thereby cause the death of cancer cells and had less toxicity to normal cells of body but iron oxide nanoparticle showed non-toxic effect on HepG2 cell line.

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Bashari, Muhammad Hasan, Fachreza Aryo Damara, Isna Nisrina Hardani, Gita Widya Pradini, Tenny Putri, and Eko Fuji Ariyanto. "Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell Line." Indonesian Journal of Cancer Chemoprevention 11, no. 2 (July 24, 2020): 90. http://dx.doi.org/10.14499/indonesianjcanchemoprev11iss2pp90-96.

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Cervical cancer is one of the most leading causes of women death. Currently, paclitaxel is still one of the main therapeutic regimens for cervical cancer patients. However, some patients developed to be paclitaxel-resistant. Hence, studies to find out the novel strategies to resolve this problem are important. Generating resistant cancer cell lines can be utilized as the potent tool to evaluate the efficacy of any therapeutic agent toward cancer drug-resistant problems. Current studies describing the methods to establish chemoresistance are lacking. Moreover, study in Indonesia conducting chemoresistance in cell line is limited. This study was aimed to elaborate the characteristics of HeLa cells during generation of paclitaxel-resistant cervical cancer cells. The parental HeLa cells were exposed to an escalating concentration of paclitaxel for a long time period. Subsequently, cells were divided into two groups for the evaluation of resistance characteristics. The values of inhibitory concentration 50 (IC50) and inhibitory concentration 90 (IC90) were analyzed using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Our data showed that the longer exposing periods of paclitaxel, the higher IC50 and IC90 values of HeLa cells are. IC90 of paclitaxel in HeLa Pac RB was increased from 69 pM, 440 pM, 2,561 pM and 10,337 pM on 0th, 1st, 2nd, 3rd and 4th months, respectively. Interestingly, the resistant cells were recovered to be paclitaxel-sensitive when they were not being continuously exposed to paclitaxel. In addition, the paclitaxel resistant cells become less sensitive against 5-FU but not doxorubicin, cisplatin and etoposide. We were able to generate cervical cancer HeLa paclitaxel-resistant cell line. These cell line could potentially be utilized for further studies in order to understand the molecular mechanisms of drug resistance in cervical cancer and as a tool for cancer drug discovery.Keywords: cervical cancer, drug resistant cell line, paclitaxel resistant cells, stepwise escalating concentration.

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Elnour, Montasir. "Selected Sudanese Medicinal Plants Induce Anticancer and Cytotoxic Effects in Cervical Cancer Cell Line." Cancer Research and Cellular Therapeutics 2, no. 3 (October 9, 2018): 01–04. http://dx.doi.org/10.31579/2640-1053/031.

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Modern pharmacology, however, relies on refined chemicals - either obtained from plants, or synthesized. This work investigated the anticancer, antioxidant and Cytotoxicity activities of N. lotus leaves citrate, N. lotus leaves not citrate, P. juliflora leaves commonly used as anti-inflammatory and Ant diabetic. All the plant parts were extracted using 80% methanol, the anticancer activity was examined by using MTT assay against Hela (Cervical Cancer) Cell Line. And determine their antioxidant activities by testing DPPH cytotoxicity using - (4, 5-Dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), filter and kept in dark, prepared freshly. The examined plants methanolic extracts of P. juliflora leaves is high activity against Hela (Cervical Cancer) Cell Line IC50 is 56.02 µg/ml. The extract C N. lotus leaves citrate, N. lotus leaves not citrate has shown none active anti- Hela (Cervical Cancer) IC50 values 100, and > 100 μg/ml respectively. All the extracts revealed cytotoxicity activity not toxic in N. lotus leaves citrate, N. lotus leaves not citrate, P. juliflora leaves the inhibition percentage with (90.81, 89.33074 , 86.47866 ) (73.17427 , 71.93975 , 60.17069 ) (74.93001, 73.78714 , 71.13981 ) respectively. The following plant parts showed highly potent scavenging activity against DPPH (above 80%); N. lotus leaves citrate, 88.78, While the following revealed a good activity against DPPH (between 60-79); N. lotus leaves not citrate, P. juliflora leaves with inhibition % are 77and 62.96 respectively.

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Chen, Yu, Jinshu Ma, Fang Wang, Jie Hu, Ai Cui, Chengguo Wei, Qing Yang, and Fan Li. "Amygdalin induces apoptosis in human cervical cancer cell line HeLa cells." Immunopharmacology and Immunotoxicology 35, no. 1 (November 9, 2012): 43–51. http://dx.doi.org/10.3109/08923973.2012.738688.

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15

AlSalhi, M. S., M. Atif, A. A. AlObiadi, and A. S. Aldwayyan. "Photodynamic damage study of HeLa cell line using ALA." Laser Physics 21, no. 4 (March 4, 2011): 733–39. http://dx.doi.org/10.1134/s1054660x11070024.

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16

Ivanković, Milena, Andrea Ćukušić, Ivana Gotić, Nikolina Škrobot, Mario Matijašić, Denis Polančec, and Ivica Rubelj. "Telomerase activity in HeLa cervical carcinoma cell line proliferation." Biogerontology 8, no. 2 (September 6, 2006): 163–72. http://dx.doi.org/10.1007/s10522-006-9043-9.

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Wijayanti, Nastiti, W. A. S. Tunjung, and Y. Setyawati. "CYTOTOXICITY AND APOPTOSIS INDUCTION BY KAFFIR LIME LEAVES EXTRACT (Citrus hystrix DC.) IN HeLa CELLS CULTURE (HUMAN CERVICAL CANCER CELL LINE)." KnE Life Sciences 2, no. 1 (September 20, 2015): 631. http://dx.doi.org/10.18502/kls.v2i1.233.

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Kaffir lime (Citrus hystrix) is a native plant of Indonesia. Nowadays the use of plants is limited as ingredients in Indonesia cuisine and aromatherapy oils for industry. Leaves of C. hystrix contain of polyphenols and essential oils thus they were possibly cytotoxic to cancer cell line. The objective of this research was to determine the cytotoxicity effect and apoptosis induced by leaves extract C. hystrix to cervical cancer cell line (HeLa cells). Methods used in this study included sampling of kaffir lime leaves, extractionusing three different solvents (ethanol, ethyl acetate, and hexane), detection of metabolite secondary compound (alkaloids, flavonoids, terpenoids, saponins, and tannins) using thin layer chromatography (TLC), cytotoxicity assay via MTT assay, and apoptosis test with double staining method (ethidium bromide-acrydine orange). Result showed kaffir lime crude extract dose dependently inhibitHeLa cells proliferation. lC50of ethanolicand ethyl acetate extract was 82,034 µg/mL and 57,845 µg/mL, respectively means cytotoxic to HeLa cells. On the other hand lC50 of hexane extract was 203,992 µg/mL which was not cytotoxic. Furthermore ethanolic and ethyl acetate extract were able to induce apoptosis of HeLa cells by increasing the number of apoptotic cells. In conclusion, kaffir lime leaves extract had cytotoxic effect and induced apoptosis. Moreover ethyl acetate extractof kaffir lime was the most potential to induce apoptosis in HeLa cells. Keywords: kaffir lime leaves (Citrus hystrix), HeLa, MTT, cytotoxicity, apoptosis.

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Landry, Jonathan J. M., Paul Theodor Pyl, Tobias Rausch, Thomas Zichner, Manu M. Tekkedil, Adrian M. Stütz, Anna Jauch, et al. "The Genomic and Transcriptomic Landscape of a HeLa Cell Line." G3: Genes|Genomes|Genetics 3, no. 8 (March 11, 2013): 1213–24. http://dx.doi.org/10.1534/g3.113.005777.

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Rahbari, Raheleh, Tom Sheahan, Vasileios Modes, Pam Collier, Catriona Macfarlane, and Richard M. Badge. "A novel L1 retrotransposon marker for HeLa cell line identification." BioTechniques 46, no. 4 (April 2009): 277–84. http://dx.doi.org/10.2144/000113089.

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Hammad Aziz, Muhammad, M. Fakhar-e-Alam, Mahvish Fatima, Fozia Shaheen, Seemab Iqbal, M. Atif, Muhammad Talha, et al. "Photodynamic Effect of Ni Nanotubes on an HeLa Cell Line." PLOS ONE 11, no. 3 (March 18, 2016): e0150295. http://dx.doi.org/10.1371/journal.pone.0150295.

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Giannoni, E., P. Cirri, P. Paoli, T. Fiaschi, G. Camici, G. Manao, G. Raugei, and G. Ramponi. "Acylphosphatase Is a Strong Apoptosis Inducer in HeLa Cell Line." Molecular Cell Biology Research Communications 3, no. 5 (May 2000): 264–70. http://dx.doi.org/10.1006/mcbr.2000.0228.

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Mendoza-Almanza, Gretel, Leticia Rocha-Zavaleta, Cecilia Aguilar-Zacarías, Jorge Ayala-Luján, and Jorge Olmos. "Cry1A Proteins are Cytotoxic to HeLa but not to SiHa Cervical Cancer Cells." Current Pharmaceutical Biotechnology 20, no. 12 (October 18, 2019): 1018–27. http://dx.doi.org/10.2174/1389201020666190802114739.

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Background: Bacillus thuringiensis toxins are effective against multiple biological targets such as insects, nematodes, mites, protozoa, and importantly, human cancer cells. One of the main mechanisms by which Cry toxins to trigger cell death is the specific recognition of cadherin-like membrane cell receptors. Objective: This work aimed to assess the cytotoxicity of the Cry1Ab and Cry1Ac toxins from Bacillus thuringiensis in HeLa, cervical cancer cell line, as well as their antitumor activity in mouse models. Methods: We analyzed several biological targets of Cry1Ab and Cry1Ac including erythrocytes, insect larvae, as well as cancer and non-cancer cell lines. The viability of HeLa, SiHa, MCF7 and HaCat cells was assessed by MTT 24 h after the administration of Cry toxins. We also studied apoptosis as a possible cytotoxicity mechanism in HeLa. The capacity of Cry toxins to eliminate tumors in xenograft mouse models was also analyzed. Results: Both toxins, Cry1Ab and Cry1Ac, showed specific cytotoxic activity in HeLa (HPV18+) cervical cancer cell line, with a Cry1Ab LC50 of 2.5 µg/ml, and of 0.5 µg/ml for Cry1Ac. Apoptosis was differentially induced in HeLa cells using the same concentration of Cry1Ab and Cry1Ac toxins. Cry1Ac eliminated 50% of the tumors at 10 µg/ml, and eliminate 100% of the tumors at 30 and 50 µg/ml. Conclusion: Bacillus thuringiensis Cry1A toxins show dual cytotoxic activity, in insects as well as in HeLa cancer cell line.

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Krizman, David B., Nancy J. Carpenter, Sen Pathak, Matilde Olivé, Relda Cailleau, and T. C. Hsu. "Hela marker chromosomes in human breast tumors: Proposal about the origin of the hela cell line." Journal of Clinical Laboratory Analysis 1, no. 1 (1987): 93–97. http://dx.doi.org/10.1002/jcla.1860010116.

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Abdallah, Lubna A., Ashraf M. Sawafta, Sanad A. Ben Ali, and Hasan A. Baradia. "Cytotoxic potential of camel whey and milk on cervix cancer (HeLa) cell line." Asian Journal of Medical and Biological Research 5, no. 3 (October 15, 2019): 231–36. http://dx.doi.org/10.3329/ajmbr.v5i3.43593.

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Camel milk is an important nutritional source that historically been used in the treatment of cancer. Therefore, the main aim of the present study is to determine the in vitro anticancer effect of both camel milk and whey against cervix cancer (HeLa) cells. To perform that, skimmed milk as well as whey immunoglobulins concentrate samples were prepared at different concentrations (0, 1, 2.5, 5, 7.5 and 10 mg/ml). Then, the in vitro effect of the prepared concentrations on HeLa cells morphology and growth was investigated by tissue culture technique. Moreover, the anticancer activity of camel milk and whey against HeLa cells was estimated by the 3-[4, 5-dimethylthiazole-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. The obtained results displayed that both camel milk and whey reduced the viability of HeLa cells specially at 7.5 and 10 mg/ml. In addition, the viability of treated HeLa cells reduced after addition of both camel milk and whey to approximately 15% in a concentration dependent manner. In conclusion, this study showed the in vitro cytotoxic effect of camel milk and whey as they inhibited the growth of HeLa cells. Asian J. Med. Biol. Res. June 2019, 5(3): 231-236

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Latypova, Diana K., Stanislav V. Shmakov, Sofya A. Pechkovskaya, Alexander S. Filatov, Alexander V. Stepakov, Nickolay A. Knyazev, and Vitali M. Boitsov. "Identification of Spiro-Fused Pyrrolo[3,4-a]pyrrolizines and Tryptanthrines as Potential Antitumor Agents: Synthesis and In Vitro Evaluation." International Journal of Molecular Sciences 22, no. 21 (November 5, 2021): 11997. http://dx.doi.org/10.3390/ijms222111997.

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A series of heterocyclic compounds containing a spiro-fused pyrrolo[3,4-a]pyrrolizine and tryptanthrin framework have been synthesized and studied as potential antitumor agents. Cytotoxicity of products was screened against human erythroleukemia (K562) and human cervical carcinoma (HeLa) cell lines. Among the screened compounds. 4a, 4b and 5a were active against human erythroleukemia (K562) cell line, while 4a and 5a were active against cervical carcinoma (HeLa) cell line. In agreement with the DNA cytometry studies, the tested compounds have achieved significant cell-cycle perturbation with higher accumulation of cells in G2/M phase and induced apoptosis. Using confocal microscopy, we found that with 4a and 5a treatment of HeLa cells, actin filaments disappeared, and granular actin was distributed diffusely in the cytoplasm in 76–91% of cells. We discovered that HeLa cells after treatment with compounds 4a and 5a significantly reduced the number of cells with filopodium-like membrane protrusions (from 63 % in control cells to 29% after treatment) and a decrease in cell motility.

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Sakuragi, A. L. Z., and E. H. Simon. "Selection and Characterization of an Interferon-responsive Clonal Cell Line of HeLa Cells." Journal of General Virology 67, no. 11 (November 1, 1986): 2355–64. http://dx.doi.org/10.1099/0022-1317-67-11-2355.

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Kim, Hyun Hee, and Gyesik Min. "Inhibitory Effects of S-allylcysteine on Cell Proliferation of Human Cervical Cancer Cell Line, HeLa." Journal of Life Science 25, no. 4 (April 30, 2015): 397–405. http://dx.doi.org/10.5352/jls.2015.25.4.397.

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Kusharyanti, Indri, Larasati Larasati, Ratna Asmah Susidarti, and Edy Meiyanto. "Hesperidin Increase Cytotoxic Activity of Doxorubicin on Hela Cell Line Through Cell Cycle Modulation and Apoptotis Induction." Indonesian Journal of Cancer Chemoprevention 2, no. 2 (June 28, 2011): 267. http://dx.doi.org/10.14499/indonesianjcanchemoprev2iss2pp267-273.

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Combination of chemotherapeutic agent and chemopreventive agent is being a new approach in cancer treatment. This is aimed at enhancing the effectivity and also reducing drug resistance and adverse side effect of the chemotherapeutic agent. Hesperidin, a citrus flavonoid has reported to reduce the proliferation of many cancer cells. The objectives of this study were to investigate cytotoxic activities, cell cycle modulation and apoptosis induction of hesperidin and its combination with doxorubicin on Hela cell lines. MTT [3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide] assay was used to measure the growth inhibitory effect of hesperidin and its combination with doxorubicin on Hela cells. Cell cycle profile was determined by flowcytometry and the data obtained was analyzed by using ModFit LT 3.0 program. Apoptosis assay was done using double staining method using ethidium-bromide and acridine-orange. Hesperidin inhibited cell growth with IC50 48 μM, while the IC50 of doxorubicin was 1000 nM. Combination of 500 nM doxorubicin and 6 μM hesperidin showed strongest inhibitory effect toward Hela cells. Hesperidin of 24 µM accumulated HeLa cells at G1 phase, but its combination with 500 nM Doxorubicin gave G1 and S phase accumulation at 24 h incubation. Both of Hesperidin and Doxorubicin were capable of inducing apoptosis. In accordance of the apoptotic effect, hesperidin, doxorubicin and their combination decreased the expression Bcl-2 and increased the expression of Bax. According to this result, hesperidin has a potency to be developed as co-chemotherapeutic agent for cervical cancer.Keywords: Cochemotherapy, Hesperidin, Doxorubicin, Hela, MTT assay

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Yurt, Fatma, Kasim Ocakoglu, Ozge Er, Hale Melis Soylu, Mine Ince, Cıgır Biray Avci, Cansu Caliskan Kurt, Fatma Aslıhan Sarı, Suleyman Gokhan Colak, and Cumhur Gunduz. "Evaluation of photodynamic therapy and nuclear imaging potential of subphthalocyanine integrated TiO2 nanoparticles in mammary and cervical tumor cells." Journal of Porphyrins and Phthalocyanines 23, no. 07n08 (July 2019): 908–15. http://dx.doi.org/10.1142/s1088424619500639.

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This study, subphthalocyanines (SubPc) and SubPc integrated TiO2 nanoparticles (SubPc-TiO[Formula: see text] were synthesized as novel photosensitizers. Their PDT effects were evaluated. Furthermore, nuclear imaging potential of [Formula: see text]I-labelled SubPc/SubPc-TiO2 were examined in mouse mammary carcinoma (EMT6) and cervix adenocarcinoma (HeLa) cell lines. The uptake results show that SubPc labelled with [Formula: see text]I radionuclide ([Formula: see text]I-SubPc) in EMT6 and HeLa cell lines was found to be approximately the same as in the WI38 cell line. However, the uptake values of SubPc-TiO2 labelled with [Formula: see text]I ([Formula: see text]I-SubPc-TiO[Formula: see text] in EMT6 and HeLa cell lines were determined to be two times higher than in the WI38 cell line. In other words, the target/non-target tissue ratio was identified as two in the EMT6 and HeLa cell lines. [Formula: see text]I-SubPc-TiO2 is promising for imaging or treatment of breast and cervix tumors. In vitro photodynamic therapy studies have shown that SubPc and SubPc-TiO2 are suitable agents for PDT. In addition, SubPc-TiO2 has higher phototoxicity than SubPc. As a future study, in vivo experiments will be held and performed in tumor-bearing nude mice.

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Stepnowski, P., A. C. Skladanowski, A. Ludwiczak, and E. Laczyńska. "Evaluating the cytotoxicity of ionic liquids using human cell line HeLa." Human & Experimental Toxicology 23, no. 11 (November 2004): 513–17. http://dx.doi.org/10.1191/0960327104ht480oa.

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This paper presents cytotoxicity data of selected imidazolium ionic liquids evaluated in vitro on the human tumor cell line HeLa. It was found that for 1-n-butyl-3-methylimidazolium entities the toxicity depends strongly on the associated anion; EC50 values are lowest for tetrafluoroborate. No direct dependence of the reduced effect concentration was found on elongating the short, methyl chain to ethyl or n-hexyl. Only for the ionic liquid with an n-decyl chain, the longest one studied, did higher hydrophobicity result in a EC50 one order of magnitude lower than that obtained with the n-butyl entity. The effect concentrations of imidazolium ionic liquids in the HeLa system used are lower than the values obtained for conventional organic solvents such as dichloromethane, toluene or xylene.

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Grinnell, B. W., D. T. Berg, and J. D. Walls. "Negative regulation of the human polyomavirus BK enhancer involves cell-specific interaction with a nuclear repressor." Molecular and Cellular Biology 8, no. 8 (August 1988): 3448–57. http://dx.doi.org/10.1128/mcb.8.8.3448.

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We have examined the cell type-specific regulation of the human BK virus (BKV) enhancer. This enhancer functions efficiently in cis to activate expression from the adenovirus major late promoter in the human kidney cell line, 293, and in a monkey kidney cell line, MK2, but not in the HeLa cell line. In gel retardation migration assays, specific BKV enhancer-protein complexes could be observed by using nuclear extracts prepared from each cell line. Moreover, a unique DNA-protein complex was observed by using the HeLa cell nuclear extracts. By DNase footprint analysis, four binding regions for HeLa cell nuclear proteins were defined within the BKV enhancer repeat region. Two of the protected regions encompassed nuclear factor 1 or CCAAT transcription factor binding sites. These nuclear factor 1 sites also were protected by nuclear proteins from the 293 and MK2 cell lines. The other two protected sites encompassed a region of symmetry which included a sequence similar to the simian virus 40 TC enhancer motif and to a conserved sequence present upstream or within the introns of several cellular genes. These two sites were not protected by either the 293 or MK2 nuclear proteins. Competition studies in transfected cells indicated that the reduced activity of the BKV enhancer in the HeLa cell line was due to negative regulation. Further, we have demonstrated that binding of a nuclear factor(s) to the HeLa cell-specific site is involved in the repression of enhancer activity.

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Grinnell, B. W., D. T. Berg, and J. D. Walls. "Negative regulation of the human polyomavirus BK enhancer involves cell-specific interaction with a nuclear repressor." Molecular and Cellular Biology 8, no. 8 (August 1988): 3448–57. http://dx.doi.org/10.1128/mcb.8.8.3448-3457.1988.

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We have examined the cell type-specific regulation of the human BK virus (BKV) enhancer. This enhancer functions efficiently in cis to activate expression from the adenovirus major late promoter in the human kidney cell line, 293, and in a monkey kidney cell line, MK2, but not in the HeLa cell line. In gel retardation migration assays, specific BKV enhancer-protein complexes could be observed by using nuclear extracts prepared from each cell line. Moreover, a unique DNA-protein complex was observed by using the HeLa cell nuclear extracts. By DNase footprint analysis, four binding regions for HeLa cell nuclear proteins were defined within the BKV enhancer repeat region. Two of the protected regions encompassed nuclear factor 1 or CCAAT transcription factor binding sites. These nuclear factor 1 sites also were protected by nuclear proteins from the 293 and MK2 cell lines. The other two protected sites encompassed a region of symmetry which included a sequence similar to the simian virus 40 TC enhancer motif and to a conserved sequence present upstream or within the introns of several cellular genes. These two sites were not protected by either the 293 or MK2 nuclear proteins. Competition studies in transfected cells indicated that the reduced activity of the BKV enhancer in the HeLa cell line was due to negative regulation. Further, we have demonstrated that binding of a nuclear factor(s) to the HeLa cell-specific site is involved in the repression of enhancer activity.

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Mondor, Isabelle, Sophie Ugolini, and Quentin J. Sattentau. "Human Immunodeficiency Virus Type 1 Attachment to HeLa CD4 Cells Is CD4 Independent and gp120 Dependent and Requires Cell Surface Heparans." Journal of Virology 72, no. 5 (May 1, 1998): 3623–34. http://dx.doi.org/10.1128/jvi.72.5.3623-3634.1998.

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ABSTRACT The binding of human immunodeficiency virus type 1 (HIV-1) (Hx10) virions to two different cell lines was analyzed by using a novel assay based on the detection, by anti-HLA-DR-specific antibodies, of HLA-DR+ virus binding to HLA-DR− cells. Virion attachment to the CD4+-T-cell line A3.01 was highly CD4 dependent in that it was potently inhibited by CD4 monoclonal antibodies (MAbs), and little virus binding to the CD4−sister A2.01 line was observed. By contrast, virion binding to HeLa cells expressing moderate or high levels of CD4 was equivalent to, or lower than, binding to wild-type CD4− HeLa cells. Moreover, several CD4 MAbs did not reduce, but enhanced, HIV-1 attachment to HeLa-CD4 cells. CD4 was required for infection of HeLa cells, however, demonstrating a postattachment role for this receptor. MAbs specific for the V2 and V3 loops and the CD4i epitope of gp120 strongly inhibited virion binding to HeLa-CD4 cells, whereas MAbs specific for the CD4bs and the 2G12 epitopes enhanced attachment. Despite this, all gp120- and gp41-specific MAbs tested neutralized infectivity on HeLa-CD4 cells. HIV-1 attachment to HeLa cells was only partially inhibited by MAbs specific for adhesion molecules present on the virus or target cells but was completely blocked by polyanions such as heparin, dextran sulfate, and pentosan sulfate. Treatment of HeLa-CD4 cells with heparinases completely eliminated HIV attachment and infection, strongly implicating cell surface heparans in the attachment process. CD4 dependence for HIV-1 attachment to target cells is thus highly cell line specific and may be replaced by other ligand-receptor interactions.

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Bhat, Firdous Ahmad, Sabeeha Shafi, Nazia Hilal, Showkat Ahmad Bhat, and Aneequa Rafiqee. "Apoptotic Effects of Prunus persica (L) Batsch Leaves against Breast Cancer Cell Line (MDA-MB-231) and Cervical Cancer Cell Line (HeLa) In Vitro." Journal of Drug Delivery and Therapeutics 10, no. 4 (July 15, 2020): 25–30. http://dx.doi.org/10.22270/jddt.v10i4.4124.

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Background: Apoptosis is a normal physiological phenomenon that plays a pivotal role during embryonic development, retention of tissue homeostasis and pathology. The experimental investigation of apoptotic processes is still challenging and routinely based on the assessment of molecular events like chromatin fragmentation and caspase enzyme activity. The present study was conducted to evaluate the apoptosis inducing effect of the Methanol, aqueous and chloroform extracts of Prunus persica leaves. Methods: Different extracts were obtained by cold extraction process using Methanol, water and Chloroform as solvents. Crude extracts were screened for different phytochemical constituents like flavonoids, tannins, sugars, saponins, and glycosides etc. The apoptotic effect of Prunus persica leaves was examined by DAPI staining assay against MDA-MB-231 (Human breast cancer cell line) and HeLa (Human cervical cancer cell line). Results: The results of the studies revealed that the Chloroform extract have tremendous apoptotic activity on MDA-MB-231 cells and methanolic extract have good apoptotic activity on HeLa cells. Nuclear morphological changes assessed by DAPI shows changes in morphology, apoptotic body formation, cell shrinkage, nuclei that were broken into discrete fragments and cell budding that resulted in cells of various sizes. Conclusion: The phytochemical screening reveals the presence of alkaloids, tannins, Saponins, steroids and flavonoids. The Chloroform extract has shown more effectiveness and less toxicity against MDA-MB-231 and Methanol extract was more apoptotic against HeLa in comparison to others. The present findings clearly indicated that Prunus persica leaves showed dose dependant cytotoxicity.

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Varalakshmi, KN, and Shruti Nair. "Anticancer, cytotoxic potential of Moringa oleifera extracts on HeLa cell line." Journal of Natural Pharmaceuticals 2, no. 3 (2011): 138. http://dx.doi.org/10.4103/2229-5119.86260.

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Huang, GuanYi, Ying Zhang, Qiang Zhang, Bin Zhang, and LongPing Wen. "Vacuolization and apoptosis induced by nano-selenium in HeLa cell line." Science China Chemistry 53, no. 11 (November 2010): 2272–78. http://dx.doi.org/10.1007/s11426-010-4116-7.

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Berg, Jörg, Barbara Doe, Kathelyn S. Steimer, and Matthias Wabl. "HeLa-LAV, an epithelial cell line stably infected with HIV-1." Journal of Virological Methods 34, no. 2 (September 1991): 173–80. http://dx.doi.org/10.1016/0166-0934(91)90097-j.

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DZIUBIŃSKA-PAROL, IZABELA, WOJCIECH PAROL, MARIUSZ SKOCZYŃSKI, ANNA GOŹDZICKA--JÓZEFIAK, ANNA SEMCZUK-SIKORA, and ANNA KWAŚNIEWSKA. "Influence of progesterone on proliferation and HPV18oncoproteins level in HeLa cell line." PRZEGLĄD GINEKOLOGICZNO-POŁOŻNICZY 5, no. 2-2 (May 4, 2005): 69–73. http://dx.doi.org/10.1066/s10014040061.

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Hamed, Fadil M., and Mohammad H. Mohammad. "Synthesis ,Characterization and invitro evaluation of anticancer activity of new hydroxamicacid basedHDACi containing substituted thaizolesas a cap linking moiety." JOURNAL OF ADVANCES IN CHEMISTRY 13, no. 10 (March 7, 2017): 5954–61. http://dx.doi.org/10.24297/jac.v13i10.5886.

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The present study was undertaken to synthesize, characterize and evaluate the anticancer activity of new derivatives of hydroxamate â€“based HDACi having S-subistituted-5-amino1,3,4thadiazole as a cap linking moiety ,with suitable aliphatic linker.The structures and purity of the targeted compounds were confirmed by TLC , FTIR ,H-NMR and mass spectroscopy and their anticancer activity were evaluated by comparative cytotoxic study , Using HeLa nuclear extract and normal embryonic fibroblasts cell lines.All the synthesized compounds shows good anticancer activity, represented by their high rate of growth inhibition on Hela cell line and low cytotocic effect on normal cell line .Compound (VAb) show the best safety index(SI) that represented by its selective cytotoxic activity on HeLa cell linewith low cytotoxic effect on normal embryonic cell line.

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WANG, KEFANG, JIANFANG ZENG, LIJING LUO, JIAXIN YANG, JIE CHEN, BIN LI, and KENG SHEN. "Identification of a cancer stem cell-like side population in the HeLa human cervical carcinoma cell line." Oncology Letters 6, no. 6 (October 7, 2013): 1673–80. http://dx.doi.org/10.3892/ol.2013.1607.

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Wang, Zhi, Wen Hui Fu, Xiang Yang Lu, and Guang Xian Cai. "Effects of Buthus martensii Karsch Venom on Cell Proliferation and Cytotoxicity in Hela Cells." Advanced Materials Research 345 (September 2011): 393–98. http://dx.doi.org/10.4028/www.scientific.net/amr.345.393.

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This paper focuses on the effect of the venom of the scorpion Buthus martensii on the proliferation of human cervical carcinoma Hela cell line and the related molecular mechanism. MTT test showed that the scorpion venom inhibited proliferation of Hela cells in time-dependent and concentration-dependent manner with 50% inhibitory concentration (IC50) of 34.5 μg/mL(48 h). By using flow cytometry, it was found that the scorpion venom could induce apoptosis and necrosis in Hela cells. RT-PCR and Western blot indicated there were obviously up-regulated in the expressions of p21 protein but the expression of p21 mRNA showed no significant difference in the Hela cell by the scorpion venom. These results suggest that the possible mechanism of the scorpion venom is to activate the expressions of p21 protein and to cause Hela cell apoptosis.

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Saxena, Shikha, Deepika Bisht, Basavaraj Sajjanar, Arvind Kumar Singh, Aditya Prasad Sahoo, Gandham Ravi Kumar, Ashok Kumar Tiwari, Satish Kumar, and Pramod Ramteke. "Intracellular Delivery of Histidine and Arginine Rich Cell Penetrating Peptides into HeLa cell Line." Journal of Animal Research 5, no. 4 (2015): 695. http://dx.doi.org/10.5958/2277-940x.2015.00116.3.

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Ruottinen, Maria, Antti Vasala, Helmut Pospiech, and Peter Neubauer. "On-line monitoring of HeLa cell growth and cell cycle in spinner flask cultures." Journal of Biotechnology 131, no. 2 (September 2007): S70. http://dx.doi.org/10.1016/j.jbiotec.2007.07.121.

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KUMAR, RAJENDRAN, and Subban Ravi. "Synthesis, Biological Valuation and Molecular Docking Analysis of New 5-Benzylidene Bis-Rhodanine Derivatives." Oriental Journal Of Chemistry 36, no. 6 (December 30, 2020): 1078–87. http://dx.doi.org/10.13005/ojc/360609.

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The synthesis of 5-benzylidene bis-rhodanine derivatives are reported from bis-rhodanine (III) and different aromatic aldehydes (IV) via Knoevenagel condensation reactions. All the Derivatives (V) and (Va-m) were deep-rooted by NMR spectroscopic techniques and elemental analysis. The antiproliferative study of the compounds on HeLa human cervical cancer cell line, K562 leukemic cell line and MDAMB231 breast cancer cell line were performed by MTT assay. Docking studies were carried out against the protein HPV 16E2 present in the HeLa cell line. It show good docking scores. The results indicate that the bis-rhodanine derivatives could serve as potential molecules for the development of new anticancer agents.

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Kim, Sung-Kuk, Soon Ok Woo, Sang Mi Han, Se Gun Kim, Kyung Won Bang, Hyo Young Kim, Hong Min Choi, and Hyo Jung Moon. "Cytotoxic Effect and Protein Expression by Korean Regional Propolis on HeLa Ovarian Cancer Cell Line." Journal of Apiculture 34, no. 3 (September 30, 2019): 245–54. http://dx.doi.org/10.17519/apiculture.2019.09.34.3.245.

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Rajan, Teena, Benluvankar V, and Vincent S. "SACCHAROMYCES CEREVISIAE-INDUCED APOPTOSIS OF MONOLAYER CERVICAL CANCER CELLS." Asian Journal of Pharmaceutical and Clinical Research 10, no. 8 (August 1, 2017): 63. http://dx.doi.org/10.22159/ajpcr.2017.v10i8.18818.

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Objective: The present study was undertaken to examine the effect of phagocytosis of killed yeast on the induction of apoptosis in monolayer of HeLa cells.Methods: HeLa cell line was incubated with different doses (1000-7.8 μg/ml) of heat-killed Saccharomyces cerevisiae for 24, 48, and 72 hrs. The cytotoxicity against HeLa cell line during different exposure hours was screened by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-tetrazolium bromide assay. Induction of apoptosis was further confirmed by morphological and biochemical examination. Antiproliferative effect of yeast was examined under inverted microscope. Cell morphological changes were analyzed by fluorescent staining with propidium iodide.Results: The results showed that yeast induces cytotoxicity against HeLa cells in concentrations and during prolonged exposure periods. The viability of HeLa cells decreased from 85% to 45% during 72 hrs of treatment with 1000 μg/ml of yeast cells. The inhibitory concentration 50% of heat-killed yeast required to induce 50% inhibition of HeLa cells was 62.5 μg/ml. Apoptotic cells showed signs such as cell enlargement, membrane blebbing, and chromatin condensation. Furthermore, cell cycle analysis showed that S. cerevisiae treated HeLa cells and showed a typical apoptosis pattern of DNA content that reflected sub-G0 phase (corresponding to apoptotic cells).Conclusion: Results from the present work show that the heat-killed yeast has anticancer activity and it includes apoptosis of HeLa cells in vitro.

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Sharma, Nitin, and Mahesh Kumar. "Expression of Bovine Viral Diarrhoea Virus E2 Gene in HeLa Cell Line." International Journal of Current Microbiology and Applied Sciences 8, no. 10 (October 10, 2019): 2145–50. http://dx.doi.org/10.20546/ijcmas.2019.810.249.

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Mathur, Shivangi, Rinkal Mulani, L. B. George, Chiranjivi Barot, Aarti Thakkar, Vibha Bhingradiya, and Ruby Patel. "ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF CommiphoraMukul(GUGGUL) EXTRACTS AGAINST HeLa CELL-LINE." International Journal of Advanced Research 4, no. 5 (May 31, 2016): 1258–67. http://dx.doi.org/10.21474/ijar01/412.

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Uzun, Kübra, Pınar İkiz, Ruziye Daşkın, Perihan Gürbüz, and Funda Nuray Yalçın. "Cytotoxic Potentials of Some Asteraceae Plants from Turkey on HeLa Cell Line." Proceedings 1, no. 10 (November 15, 2017): 1068. http://dx.doi.org/10.3390/proceedings1101068.

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Qian, Ying, Yingnian Yu, Xingruo Cheng, Jianhong Luo, Haiyang Xie, and Binghui Shen. "Molecular events after antisense inhibition of hMSH2 in a HeLa cell line." Mutation Research/Genetic Toxicology and Environmental Mutagenesis 418, no. 2-3 (October 1998): 61–71. http://dx.doi.org/10.1016/s1383-5718(98)00108-9.

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Journal articles on the topic 'HeLa cell line'

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