Relevant bibliographies by topics / HeLa cell line

Academic literature on the topic 'HeLa cell line'

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Published: 4 June 2021
Last updated: 25 April 2022

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Journal articles on the topic "HeLa cell line"

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Beard, Jonathan. "A cell line called HeLa." New Scientist 205, no. 2748 (February 2010): 47. http://dx.doi.org/10.1016/s0262-4079(10)60428-9.

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Callaway, Ewen. "Deal done over HeLa cell line." Nature 500, no. 7461 (August 2013): 132–33. http://dx.doi.org/10.1038/500132a.

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Mandia, Sayati. "Cytotoxicity activity of Liverwort (Marchantia Polymorpha L.)Methanolic Extract to HeLa cell Line." Asian Pacific Journal of Health Sciences 7, no. 1 (March 30, 2020): 69–73. http://dx.doi.org/10.21276/apjhs.2020.7.1.13.

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Ekowati, Heny, Indwiani Astuti, and Mustofa Mustofa. "ANTICANCER ACTIVITY OF CALANONE ON HeLa CELL LINE." Indonesian Journal of Chemistry 10, no. 2 (July 21, 2010): 240–44. http://dx.doi.org/10.22146/ijc.21467.

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Calanone (coumarin derivate compound), isolated from Calophyllum sp. had been shown to have cytotoxic activity on leukemia L1210 cell line with IC50 = 59.40 mg/mL. Calanone presumed have anticancer activity on HeLa cervical carcinoma cell. This study was conducted to investigate the cytotoxic and apoptotic activity of calanone and its effect to p53 and p21 expression on HeLa cervical carcinoma cell. Cytotoxic assay of calanone was performed on HeLa cell line, using MTT assay. Apoptotic assay was performed on HeLa cell line incubated with calanone for 24 h, by immunofluororescence method, using fluorochromes ethidium bromide and acridine orange. Expression of p53 was examined on HeLa cell line, by PCR with p53 wild-type primer. Expression of p21 was examined on HeLa cell line, by immunohistochemistry method. 5-fluorourasil was used as positive control in cytotoxic, apoptotic assay, and p53 expression. The result showed that calanone has cytotoxic activity on HeLa cell line, with IC50 = 22.887 mg/mL, caused cytotoxicity through apoptotic mechanism, increase p53 tumor suppressor gene expression, while the p21 expression test showed a negative result. Keywords: Calanone, cytotoxic, HeLa cell line
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Krizman, D. B., S. Pathak, and R. Cailleau. "Double minutes in the HeLa cell line." Cancer Genetics and Cytogenetics 18, no. 1 (September 1985): 43–47. http://dx.doi.org/10.1016/0165-4608(85)90038-x.

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Fountoulakis, Michael, George Tsangaris, Ji-eun Oh, Antony Maris, and Gert Lubec. "Protein profile of the HeLa cell line." Journal of Chromatography A 1038, no. 1-2 (June 2004): 247–65. http://dx.doi.org/10.1016/j.chroma.2004.03.032.

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Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins. "Henrietta Lacks, HeLa Cells, and Cell Culture Contamination." Archives of Pathology & Laboratory Medicine 133, no. 9 (September 1, 2009): 1463–67. http://dx.doi.org/10.5858/133.9.1463.

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Abstract Henrietta Lacks died in 1951 of an aggressive adenocarcinoma of the cervix. A tissue biopsy obtained for diagnostic evaluation yielded additional tissue for Dr George O. Gey's tissue culture laboratory at Johns Hopkins (Baltimore, Maryland). The cancer cells, now called HeLa cells, grew rapidly in cell culture and became the first human cell line. HeLa cells were used by researchers around the world. However, 20 years after Henrietta Lacks' death, mounting evidence suggested that HeLa cells contaminated and overgrew other cell lines. Cultures, supposedly of tissues such as breast cancer or mouse, proved to be HeLa cells. We describe the history behind the development of HeLa cells, including the first published description of Ms Lacks' autopsy, and the cell culture contamination that resulted. The debate over cell culture contamination began in the 1970s and was not harmonious. Ultimately, the problem was not resolved and it continues today. Finally, we discuss the philosophical implications of the immortal HeLa cell line.
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Haridas, Renjini. "IN-VITRO CYTOTOXICITY ACTIVITY OF MALAXIS RHEEDEI SW METHANOL EXTRACT AGAINST HELA CELL LINE AND MCF- 7 CELL LINE." Asian Journal of Pharmaceutical and Clinical Research 9, no. 6 (November 1, 2016): 244. http://dx.doi.org/10.22159/ajpcr.2016.v9i6.14298.

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Cancer is a group of diseases caused by loss of cell cycle control. Cancer is associated with abnormal uncontrolled cell growth. The study was aimed to evaluation of the anticancer activity of the Malaxis rheedei Sw. on the HeLa cell line and MCF- 7cell line. The whole plant part of the Malaxis rheedei methanolic extract were tested for its inhibitory effect on HeLa Cell Line and MCF- 7cell line. The cytotoxicity of Malaxis rheedei on HeLa cell and MCF- 7cell line were evaluated by the MTT assay. Malaxis rheedei methanolic extract has significant cytotoxicity effect on MCF- 7cell line in concentration range between 18.75 to 300 µg/ml by using MTT assay and study also showed that inhibitory action on HeLa cell line inconcentration range between 18.75 to 300 µg/ml by using MTT assay. Methanol extract of the whole plant part of Malaxis rheedei was found to be 7.3%, 16.6%, 25.4%, 36.3% and 47.1%toxic in HeLa cell line and 7.9%, 13.9%, 26%, 48.4% and 66.3% toxic in MCF- 7cell line. IC50 value of Malaxis rheedei on MCF- 7cell was 167.76 µg/ml and IC50 value of Malaxis rheedei on HeLa Cell was not found by MTT assay. From the performed assay, methanol extract of these drug shows greater activity on MCF- 7cell line and little activity on HeLa cell line and that mean Malaxis rheedei can be used as anticancer activity.Keywords: Cytotoxicity Activity, MTT Assay, Malaxis rheedei Sw. , HeLa CellLine, MCF- 7Cell Line.
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Wang, Ching-Chiung, Lih-Geeng Chen, and Ling-Ling Yang. "Camelliin B induced apoptosis in HeLa cell line." Toxicology 168, no. 3 (November 2001): 231–40. http://dx.doi.org/10.1016/s0300-483x(01)00452-8.

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Fakhar-e-Alam, M., S. Kishwar, Y. Khan, M. Siddique, M. Atif, O. Nur, and M. Willander. "Tumoricidal effects of nanomaterials in HeLa cell line." Laser Physics 21, no. 11 (September 2, 2011): 1978–88. http://dx.doi.org/10.1134/s1054660x1119011x.

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Dissertations / Theses on the topic "HeLa cell line"

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Haji, Zulkipli Ihsan Nazurah. "MARK2/PAR-1b/EMK1 is required for spindle centring in the epithelial cell line HeLa." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708195.

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Athanassiou-Papaefthymiou, Maria G. "Functional activation of the p53 tumor suppressor in non-tumorigenic variants of the HeLa cervical carcinoma cell line." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/39376.

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Di, Guilmi Anne-Marie. "Étude de l'interaction de l'adénovirus humain de sérotype 3 avec les cellules HeLa." Université Joseph Fourier (Grenoble ; 1971-2015), 1994. http://www.theses.fr/1994GRE10058.

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Les travaux decrits dans ce rapport concernent l'interaction de l'adenovirus humain de serotype 3 avec les cellules hela. Dans un premier temps, nous avons defini les caracteristiques de la liaison du virus ad3 avec les cellules hela a 37c et a 4c. Pour ces deux conditions de temperature, nous avons montre que le virus ad3 a la propriete de se lier avec deux affinites distinctes sur ses sites recepteurs. Nous avons demontre que les virus ad2 et ad3 (appartenant respectivement aux sous-groupes c et b) ne partagent pas les memes sites de liaison a la surface des cellules hela. La fibre est la proteine virale dont la fonction est de se lier au recepteur. Nous avons produit cette proteine dans le systeme de baculovirus. La fibre ad3 recombinante presente une morphologie et une structure correctes. L'activite fonctionnelle de la fibre a ete mise en evidence par l'efficacite de l'inhibition de la fixation du virus ad3, a la surface des cellules hela. Dans le but d'identifier le recepteur cellulaire de l'ad3 nous avons applique la technique du vopba en utilisant la fibre ad3 recombinante et le virus ad3. A partir des cellules hela, le virus et la fibre ad3 ont detecte une proteine membranaire de 130kda avec laquelle ils interagissent. Cette reconnaissance presente un caractere specifique. Des travaux similaires effectues sur des cellules a549, permissives a l'infection par le virus ad3, n'ont cependant pas permis de detecter cette proteine 130
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Lizama, Natalia. "Afterlife, but not as we know it : medicine, technology and the body resurrected." University of Western Australia. School of Social and Cultural Studies, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0186.

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This thesis contends that technologically-derived resurrections of human bodies and bodily fragments can be viewed as indicative of a 'post-biological' ontology. Drawing from examples in which human bodies are resurrected, both figuratively and actually, this thesis puts forward the term 'post-biological subject' as an ideological framework for conceptualising the reconfiguration of human ontology that results from various medical technologies that 'resurrect' the human body. In this instance, the term 'postbiological', borrowed from Hans Moravec who uses it denote a future in which human being is radically disembodied and resurrected within a digital realm, is used somewhat ironically: where Moravec imagines an afterlife in which the body is discarded as so much 'meat', the post-biological afterlife of the body in this thesis centres around a form of corporeal resurrection. Corpses, living organs and excreta may all be resurrected, some of them in digital format, yet this kind of resurrection departs radically from the disembodied spiritual bliss imagined in many conceptualisations of resurrection. The post-biological subject resists ontological delineation and problematises boundaries defining self and other, living and dead, and human and nonhuman and is fraught with a number of cultural anxieties about its unique ontological status. These concerns are analysed in the context of a number of phenomena, including melancholy, horror, monstrosity and the uncanny, all of which similarly indicate an anxious fixation with human ontology. The purpose of discussing post-biological bodies in relation to phenomena such as melancholy or the uncanny is not to reinstate as ideological frameworks the psychoanalytic models from which these concepts are derived, but rather to use them as starting points for more complex analyses of postbiological ontology. The first and second chapters of this thesis discuss instances in which the human body is posthumously modified, drawing on Gunther von Hagens's Body Worlds exhibition and the Visible Human Project. The Body Worlds plastinates are situated in a liminal and ambiguous ontological space between life and death, and it is argued that their extraordinary ontological status evokes a form of imagined melancholy, wherein the longed-for and lost melancholic object is a complete process of death. In the case of the Visible Human Project, it is argued that the gruesome and highly technologised process of creating the Visible Male, wherein the corpse is effectively dehumanised and iv rendered geometric, evokes the trope of horror, while at the same time being fraught with a nostalgic longing for a pre-technological, anatomically 'authentic' body. The third and fourth chapters of this thesis discuss instances in which the living human body is reconfigured, focusing on immortal cell lines and organ transplantation, and on medical imaging technologies such as computed tomography and magnetic resonance imaging. In the third chapter it is argued that organ transplantation and the creation of immortal cell lines give rise to profound anxieties about ontological contamination through their capacity to render permeable the imagined boundaries defining self, and in this way invoke the monstrous. The fourth chapter interrogates the representation of medical imaging in Don DeLillo?s novel White Noise, arguing that the medical representation of the body functions as a form of double, a digital doppelganger that elicits an uncanny anxiety through its capacity to presage death.
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To, Wing Shu. "Effect of cellular redox and energy states on benzo[a]pyrene induced modes of death in the hepa and the HepG2 cell lines." HKBU Institutional Repository, 2010. http://repository.hkbu.edu.hk/etd_ra/1173.

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Zhang, Jianqiang. "PERMISSIVENESS OF SELECTED CELL LINES TO EQUINE ARTERITIS VIRUS: ESTABLISHMENT, CHARACTERIZATION, AND SIGNIFICANCE OF PERSISTENT INFECTION IN HELA CELLS." Lexington, Ky. : [University of Kentucky Libraries], 2005. http://lib.uky.edu/ETD/ukyvesc2005d00339/dissertation.pdf.

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Thesis (Ph. D.)--University of Kentucky, 2005.
Title from document title page (viewed on November 18, 2005). Document formatted into pages; contains ix, 222 p. : ill. Includes abstract and vita. Includes bibliographical references (p. 200-220).
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Maurer, Barry James. "Dihydrofolate reductase gene amplification in human cell lines VA2-B and hela BU25." Diss., Pasadena, Calif. : California Institute of Technology, 1995. http://resolver.caltech.edu/CaltechETD:etd-10232007-082738.

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Rinehart, Janet Emilea. "Development of HeLa cell lines that differentiate human rhinoviruses using the major cellular receptor /." The Ohio State University, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=osu148784410597496.

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Robeson, Kalen Z. "Development of a Fast and Accurate Mutation Assay in Human Cell Lines." Ohio University Honors Tutorial College / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors149270391894463.

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Klaus, Stephen J. "Analysis of B Cell Immediate Early Gene Expression in Response to Contact Dependent T Cell Help and Anti-immunoglobulins: a Thesis." eScholarship@UMMS, 1991. http://escholarship.umassmed.edu/gsbs_diss/220.

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B cells get help in the antibody response by presenting processed antigen to helper T cells. We asked whether the antigen presenting B cell must induce T helper functions before receiving help, or whether B cell activation is a direct consequence of T cell antigen recognition on the B cell surface. Although antigen-dependent increases in B cell c-myc expression occur as early as two hours after conjugation, the B cell response depends on induction of a contact-dependent helper function in the T cell, which is inhibitable by cyclosporin A. Induction but not delivery of contact help is blocked by anti-class II MHC antibody, indicating that the delivery of T cell help is not Ag dependent or MHC restricted. Also, contact with activated helper T cells induces a different pattern of immediate early gene expression from signals transduced through the B cell antigen receptor. Egr-1 is rapidly upregulated in response to mitogenic signals induced by receptor crosslinking on murine B lymphocytes, and its expression closely correlates with B cell proliferation in several models of B cell activation and tolerance. We compared egr-1 expression during B cell stimulation with Fab'2 and IgG anti-Ig, since it is known that Fab'2 anti-Ig is mitogenic while IgG is not, due to a dominant inhibitory effect of crosslinking the B cell FcγRII to membrane Ig. While mitogenic doses of Fab'2 anti-Ig induce large and rapid increases in egr-1 expression, intact anti-Ig results in only small increases in egr-1 mRNA, comparable to that seen with submitogenic concentrations of Fab'2 anti-Ig. However, when IL-4 is added as a comitogen to induce B cell proliferation with submitogenic concentrations of Fab'2 anti-Ig or IgG anti-Ig, no concomitant increases in egr-1 are observed. The regulation of egr-1 therefore, is similar to that of c-myc in this system, since neither correlates with IL-4 induced DNA synthesis.
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Books on the topic "HeLa cell line"

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Sleator, William. Hell phone. New York: Amulet Books, 2006.

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European Society of Animal Cell Technology., International Association of Biological Standardization., Fondazione iniziative zooprofilattiche (Brescia, Italy), and Fondazione internazionale Menarini, eds. Proceedings of the Joint ESACT/IABS Meeting on the Production and Exploitation of Existing and New Animal Cell Substrates, held at Villa Alba, Gardone Riviera, Italy, 21-25 May 1984. Basel: S. Karger, 1985.

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European Society of Animal Cell Technology., ed. Proceedings of the 7th General Meeting [of ESACT] on Advances in Animal Cell Technology: Cell Engineering, Evaluation and Exploitation, held ... in Baden near Vienna, Austria, on September 30th to October 4th, 1985. Basel: S. Karger, 1987.

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Wilson-Friesen, Heather Louise. Biological characterization of fastidious enteric adenoviruses types 40 and 41 in 293 and HeLa cell lines. Ottawa: National Library of Canada, 1995.

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Sleator, William. Hell phone. New York: Amulet Books, 2006.

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Skloot, Rebecca. The immortal life of Henrietta Lacks. Detroit: Large Print Press/Gale Cengage Learning, 2011.

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Skloot, Rebecca. The immortal life of Henrietta Lacks. New York: Broadway Paperbacks, 2011.

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Skloot, Rebecca. The immortal life of Henrietta Lacks. New York: Broadway Paperbacks, 2011.

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Skloot, Rebecca. The immortal life of Henriette Lacks. Waterville, Me: Thorndike Press, 2010.

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The Immortal Life of Henrietta Lacks. New York: Crown Publishing Group, 2010.

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Book chapters on the topic "HeLa cell line"

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Degouys, V., C. Gatot, S. Sonderhoff, F. Trampler, and A. O. A. Miller. "2D PAGE examination of the proteins extracted from high density perfused HeLa cell cultures controlled by an on-line capacitance-based biomass probe." In Animal Cell Technology, 243–49. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5404-8_38.

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Venkatachalam, P., T. Bhuvaneswari, and N. Geetha. "Green Engineering of Silver Nanoparticles Using Leucas aspera Extract: Cytotoxic Efficacy in HeLa Cell Line." In Nanotechnology in the Life Sciences, 333–46. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-65792-5_13.

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SenthilKannan, K., G. Flora, S. Gunasekaran, H. Abdul Jaffar Ali, M. Vimalan, and S. Balasubramanian. "Extraction of Silver Nanoparticles (Ag-NPs) by Green Synthesis from Aqueous Extract of Seaweeds and Their Consequences on HeLa Cell Line and Their Utility on Soil by Spectroscopic Tools." In Nanotechnology for Advances in Medical Microbiology, 119–38. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-9916-3_5.

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Glass, Bertram, and Marie José Kersten. "Diffuse Large B Cell Lymphoma and Primary Mediastinal Lymphoma." In The EBMT/EHA CAR-T Cell Handbook, 67–74. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-94353-0_12.

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AbstractThe outcome of patients with large B cell lymphoma (LBCL) who did not respond to a classical immunochemotherapy regimen at any time or relapsed within 1 year following chemoimmunotherapy is poor. The Scholar-One-Study showed long-term event-free survival for less than 20% of these patients (Crump et al. 2017). The introduction of chimeric antigen receptor T cell therapy (CAR-T) is a substantial advancement for these patients, offering long-term remission and a curative prospect for 30 to 40% of patients (summarized in Table 12.1), (Abramson et al. 2020; Neelapu et al. 2017; Schuster et al. 2019b). To date, in Europe, two products (axicabtagene ciloleucel and tisagenlecleucel) have been licenced by the European Medical Agency, and a third product (lisocabtagene maraleucel) will become available in 2021. All these products are licenced for patients who have failed at least two prior lines of systemic therapy. This initially defines, however broad, a range of possible situations in which the application of CART is indicated. The following considerations may help to further define the patient population that should be offered CAR-T cells as the next line of treatment. Studies addressing the potential benefit of CAR-T cells compared to high-dose chemotherapy and autologous stem cell transplantation for second-line treatment of LBCL have been fully recruited, but the results are still pending.
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Lu, Xiyuan, Cuihong Dai, Aiju Hou, Jie Cui, Dayou Cheng, and Dechang Xu. "Dysregulated microRNA Profile in HeLa Cell Lines Induced by Lupeol." In Bioinformatics Research and Applications, 71–80. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-08171-7_7.

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Tsukita, Sachiko, Tomoki Yano, and Elisa Herawati. "Apical Cytoskeletons Help Define the Barrier Functions of Epithelial Cell Sheets in Biological Systems." In Make Life Visible, 31–38. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-7908-6_4.

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Shaik, Ilahi, A. Shameem, and P. Sasi Bhushana Rao. "Anti-cancer Activity of Selected Seaweeds Against HeLa, K-562 and MDA-MB Cell Lines." In SpringerBriefs in Applied Sciences and Technology, 35–42. Singapore: Springer Singapore, 2014. http://dx.doi.org/10.1007/978-981-287-050-6_4.

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Kanai, Yoshiyuki, and Sei-Ichi Tanuma. "Immunofluorescent Staining of Poly(ADP-Ribose) in Situ in Acetone-Fixed HeLa Cells: Its Usefulness for Clinical and Basic Sciences." In Proceedings in Life Sciences, 496–501. Berlin, Heidelberg: Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-70589-2_70.

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Kamala Priya, M. R., and Priya R. Iyer. "Apoptotic Activity in Cervical Cancer HeLa Cell Lines Treated with Chitosan Nanoconjugated Drug Doxorubicin – as Nanocarrier for Drug Delivery." In Proceedings of the International Conference on Nanomedicine (ICON-2019), 42–53. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-25135-2_5.

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Zwart, Hub. "Philosophy of Technoscience: From Cis-Continental to Trans-Continental." In Philosophy of Engineering and Technology, 229–45. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-84570-4_8.

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AbstractThe previous chapters explored how four (interacting and overlapping) continental approaches (dialectics, dialectical materialism, psychoanalysis and phenomenology) offer hints and guidance for coming to terms with the revolutionary dynamics and disruptive impact of contemporary technoscience. Hegelian dialectics provides a conceptual scaffold for developing a comprehensive view of the terrestrial system and even for addressing the Cambrian explosion currently unfolding in laboratories around the globe, as a result of technoscientific developments such as synthetic biology and CRISP-Cas9. Dialectical materialism likewise offers a conceptual framework for addressing the rapidly aggravating disruption of the metabolism between nature and global civilisation, and the ongoing convergence of biosphere and technosphere, exemplified by the synthetic cell. Francophone psychoanalysis, closely aligned with dialectical thinking, adds to our understanding of the specificity of technoscience, both as a practice and as a discourse, where technoscientific research emerges as a questionable vocation driven by a desire to control, but at the same time ostensibly out of control. The dialectical methodology of psychoanalysis was exemplified with the help of case histories, moreover, involving Majorana particles, gene drives, malaria mosquitoes and nude mice. The latter represent technoscientific commodities, exemplifying the assembly-line production of human-made organisms (the commodification of life as such). Subsequently, we demonstrated how Heideggerian phenomenology entails important methodological hints for understanding technoscientific artefacts against the backdrop of technoscience as a mobilising force and as a global enterprise. And finally, we outlined how Teilhard’s views on the genesis of consciousness, self-consciousness and hyperconsciousness retrieve the historical (dialectical) dimension of phenomenology, thus allowing us to assess the present as a global unfolding of the noosphere.
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Conference papers on the topic "HeLa cell line"

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Zhao, Zhikun, Jing Tu, Zuhong Lu, and Shiping Liu. "Dominant Isoform in Alternative Splicing in HeLa S3 Cell Line Revealed by Single-cell RNA-seq." In CSBio '16: 7th International Conference on Computational Systems-Biology and Bioinformatics. New York, NY, USA: ACM, 2016. http://dx.doi.org/10.1145/3029375.3029376.

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Thant, Tin Myo, Nanik Siti Aminah, Alfinda Novi Kristanti, Rico Ramadhan, Hnin Thanda Aung, and Yoshiaki Takaya. "Cytotoxic Carbazole Alkaloid from the Root of Clausena cxcavata on Hela Cell Line." In International Conference on Chemical Science and Technology Innovation. SCITEPRESS - Science and Technology Publications, 2019. http://dx.doi.org/10.5220/0008858101410144.

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Juárez, Alfonso Antonio Sequeda, Elizabeth Maldonado Alvarado, and Eva Ramón Gallegos. "Cell death induced by photodynamic therapy with the conjugate of gold nanoparticles-PpIX in HeLa cell line." In 1ST INTERNATIONAL CONFERENCE ON BIOINFORMATICS, BIOTECHNOLOGY, AND BIOMEDICAL ENGINEERING (BIOMIC 2018). Author(s), 2019. http://dx.doi.org/10.1063/1.5095911.

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Nikolic, Ivana, Jovan Lukovic, Marina Mitrovic, Zoran Ratković, Jovana Muškinja, Marija Anđelković, Marina Živić, and Tanja Zečević-Luković. "Vanillin-based chalcone analogue induces cell death in HeLa and HCT-116 cell line through mitochondrial apoptotic pathway." In 6th International Electronic Conference on Medicinal Chemistry. Basel, Switzerland: MDPI, 2020. http://dx.doi.org/10.3390/ecmc2020-07429.

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Ramon Gallegos, Eva, Salomon Hernandez Guitierrez, Leticia Garduno Siciliano, Jose L. Jiminez Perez, Aura J. Perez Zapata, and Alfredo Cruz Orea. "Photodynamic therapy in a cervico-uterine cancer cell line (HeLa) implanted in nu/nu mice." In EOS/SPIE European Biomedical Optics Week, edited by Patrick Brouwer. SPIE, 2001. http://dx.doi.org/10.1117/12.413721.

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"Evaluation of Caspase 9 Activity Level in Hela Cell Line Exposed to Cytotoxic Dose of Aspirin." In AEBMS-2017, ICCET-2017, BBMPS-17, UPACEE-17, LHESS-17, TBFIS-2017, IC4E-2017, AMLIS-2017 & BEFM-2017. Higher Education and Innovation Group (HEAIG), 2018. http://dx.doi.org/10.15242/heaig.c1217231.

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"The Effect of Cytotoxic Dose of Diclofenac on APC Gene Expression in Cervical Cancer (Hela) Cell Line." In AEBMS-2017, ICCET-2017, BBMPS-17, UPACEE-17, LHESS-17, TBFIS-2017, IC4E-2017, AMLIS-2017 & BEFM-2017. Higher Education and Innovation Group (HEAIG), 2018. http://dx.doi.org/10.15242/heaig.c1217227.

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Kumar, Sunil, Amita Daverey, and Dhirendra Bhahdur. "Abstract A120: Morphological assessment of apoptosis in HeLa cervical cancer cell line by Dox-loaded magnetic nanocluster." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics--Nov 12-16, 2011; San Francisco, CA. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1535-7163.targ-11-a120.

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Huang, Yiling, Liming Huang, Chengcheng You, and HuoJun Hu. "Endoplasmic Reticulum Stress Signaling Pathways Is Involved in Trichosanthin-Induced Apoptosis in Human Cervical Cancer Cell Line Hela." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5517650.

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Majeed, Shahnaz. "Plant mediated synthesis of silver nanoparticles and study of its bactericidal and anticancer activity against Hela cell line." In Proceedings of the International Conference on Nanotechnology for Better Living. Singapore: Research Publishing Services, 2016. http://dx.doi.org/10.3850/978-981-09-7519-7nbl16-rps-275.

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Reports on the topic "HeLa cell line"

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Carpita, Nicholas C., Ruth Ben-Arie, and Amnon Lers. Pectin Cross-Linking Dynamics and Wall Softening during Fruit Ripening. United States Department of Agriculture, July 2002. http://dx.doi.org/10.32747/2002.7585197.bard.

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Our study was designed to elucidate the chemical determinants of pectin cross-linking in developing fruits of apple and peach and to evaluate the role of breakage cross-linkages in swelling, softening, and cell separation during the ripening. Peaches cell walls soften and swell considerably during the ripening, whereas apples fruit cells maintain wall firmness but cells separate during late stages of ripening. We used a "double-reduction" technique to show that levels of non-methyl esters of polyuronic acid molecules were constant during the development and ripening and decreased only in overripe fruit. In peach, methyl and non-methyl esters increased during the development and decreased markedly during the ripening. Non-methyl ester linkages in both fruit decreased accompanied fruit softening. The identity of the second component of the linkage and its definitive role in the fruit softening remain elusive. In preliminary examination of isolated apples cell walls, we found that phenolic compounds accumulate early in wall development but decrease markedly during ripening. Quantitative texture analysis was used to correlate with changes to wall chemistry from the fresh-picked ripe stage to the stage during storage when the cell separation occurs. Cell wall composition is similar in all cultivars, with arabinose as the principal neutral sugar. Extensive de-branching of these highly branched arabinans pre-stages softening and cell-cell separation during over-ripening of apple. The longer 5-arabinans remain attached to the major pectic polymer rhamnogalacturonan I (RG I) backbone. The degree of RG I branching, as judged from the ratios of 2-Rha:2,4-Rha, also decreases, specially after an extensive arabinan de-branching. Loss of the 4-Rham linkages correlated strongly with the softening of the fruit. Loss of the monomer or polymer linked to the RG I produce directly or indirectly the softening of the fruit. This result will help to understand the fruit softening and to have better control of the textural changes in fruit during the ripening and especially during the storage. 'Wooliness', an undesirable mealy texture that is induced during chilling of some peach cultivars, greatly reduces the fruit storage possibilities. In order to examine the hypothesis that the basis for this disorder is related to abnormality in the cell wall softening process we have carried out a comparative analysis using the resistant cultivar, Sunsnow, and a sensitive one, Hermosa. We investigated the activity of several pectin- and glycan-modifying enzymes and the expression of their genes during ripening, chilling, and subsequent shelf-life. The changes in carbohydrate status and in methyl vs. non-methyl uronate ester levels in the walls of these cultivars were examined as well to provide a basis for comparison of the relevant gene expression that may impact appearance of the wooly character. The activities of the specific polygalacturonase (PGase) and a CMC-cellulase activities are significantly elevated in walls of peaches that have become wooly. Cellulase activities correlated well with increased level of the transcript, but differential expression of PGase did not correspond with the observed pattern of mRNA accumulation. When expression of ethylene biosynthesis related genes was followed no significant differences in ACC synthase gene expression was observed in the wooly fruit while the normal activation of the ACC oxidase was partially repressed in the Hermosa wooly fruits. Normal ripening-related loss of the uronic acid-rich polymers was stalled in the wooly Hermosa inconsistent with the observed elevation in a specific PGase activity but consistent with PG gene expression. In general, analysis of the level of total esterification, degree of methyl esterification and level of non-methyl esters did not reveal any major alterations between the different fruit varieties or between normal and abnormal ripening. Some decrease in the level of uronic acids methyl esterification was observed for both Hermosa and Sunsnow undergoing ripening following storage at low temperature but not in fruits ripening after harvest. Our results support a role for imbalanced cell wall degradation as a basis for the chilling disorder. While these results do not support a role for the imbalance between PG and pectin methyl esterase (PME) activities as the basis for the disorder they suggest a possible role for imbalance between cellulose and other cell wall polymer degradation during the softening process.
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Catalano, Jennifer G., James J. Valdes, Darrel Menking, Kyle Hubbard, and Averell L. Gnatt. Standardization of the 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium, Inner Salt (MTS) Assay for the SK-N-SH, KYSE-30, MCF-7, and HeLa Cell Lines. Fort Belvoir, VA: Defense Technical Information Center, July 2006. http://dx.doi.org/10.21236/ada452182.

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Crisosto, Carlos, Susan Lurie, Haya Friedman, Ebenezer Ogundiwin, Cameron Peace, and George Manganaris. Biological Systems Approach to Developing Mealiness-free Peach and Nectarine Fruit. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7592650.bard.

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Peach and nectarine production worldwide is increasing; however consumption is flat or declining because of the inconsistent eating quality experienced by consumers. The main factor for this inconsistent quality is mealiness or woolliness, a form of chilling injury that develops following shipping periods in the global fruit market today. Our research groups have devised various postharvest methods to prolong storage life, including controlled atmosphere and delayed storage; however, these treatments only delay mealiness. Mealiness texture results from disruption of the normal ripening process involving disassembly of cell wall material, and creates a soft fruit texture that is dry and grainy instead of juicy and smooth. Solving this problem is a prerequisite for increasing the demand for fresh peach and nectarine. Two approaches were used to reveal genes and their associated biochemical processes that can confer resistance to mealiness or wooliness. At the Volcani Center, Israel, a nectarine cultivar and the peach cultivar (isogenetic materials) from which the nectarine cultivar spontaneously arose, and at the Kearney Agricultural Center of UC Davis, USA, a peach population that segregates for quantitative resistance to mealiness was used for dissecting the genetic components of mealiness development. During our project we have conducted research integrating the information from phenotypic, biochemical and gene expression studies, proposed possible candidate genes and SNPs-QTLs mapping that are involved in reducing peach mealiness susceptibility. Numerous genes related to ethylene biosynthesis and its signal transduction, cell wall structure and metabolism, stress response, different transcription factor families were detected as being differentially accumulated in the cold-treated samples of these sensitive and less sensitive genotypes. The ability to produce ethylene and keep active genes involved in ethylene signaling, GTP-binding protein, EIN-3 binding protein and an ethylene receptor and activation of ethyleneresponsive fruit ripening genes during cold storage provided greater resistance to CI. Interestingly, in the functional category of genes differentially expressed at harvest, less chilling sensitive cultivar had more genes in categories related to antioxidant and heat sock proteins/chaperones that may help fruit to adapt to low temperature stress. The specific objectives of the proposed research were to: characterize the phenotypes and cell wall components of the two resistant systems in response to mealiness- inducing conditions; identify commonalities and specific differences in cell wall proteins and the transcriptome that are associated with low mealiness incidence; integrate the information from phenotypic, biochemical, and gene expression studies to identify candidate genes that are involved in reducing mealiness susceptibility; locate these genes in the Prunus genome; and associate the genes with genomic regions conferring quantitative genetic variation for mealiness resistance. By doing this we will locate genetic markers for mealiness development, essential tools for selection of mealiness resistant peach lines with improved fruit storability and quality. In our research, QTLs have been located in our peach SNPs map, and proposed candidate genes obtained from the integrated result of phenotypic, biochemical and gene expression analysis are being identified in our QTLs as an approach searching for consistent assistant markers for peach breeding programs.
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Spiegel, Yitzhak, Michael McClure, Itzhak Kahane, and B. M. Zuckerman. Characterization of the Phytophagous Nematode Surface Coat to Provide New Strategies for Biocontrol. United States Department of Agriculture, November 1995. http://dx.doi.org/10.32747/1995.7613015.bard.

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Chemical composition and biological role of the surface coat (SC) of the root-knot nematodes, Meloidogyne spp. are described. SC proteins of M. incognita race 3 infective juveniles (J2) were characterized by electrophoresis and western blotting of extracts from radioiodine and biotin-labelled nematodes. J2 labelled with radioiodine and biotin released 125I and biotin-labelled molecules into water after 20 hours incubation, indicating that SC proteins may be loosely attached to the nematode. Antiserum to the principal protein reacted with the surface of live J2 and with surface proteins previously separated by electrophoresis. Human red blood cells (HRBC) adhered to J2 of several tylenchid nematodes over the entire nematode body. HRBC adhered also to nylon fibers coated with SC extracted from M. javanica J2; binding was Ca++/Mg++ dependent, and decreased when the nylon fibers were coated with bovine serum albumin, or pre-incubated with fucose and mannose. These experiments support a working hypothesis that RBC adhesion involves carbohydrate moieties of HRBC and carbohydrate-recognition domain(s) (CRD) distributed on the nematode surface. To our knowledge, this is the first report of a surface CRD i the phylum Nematoda. Gold-conjugated lectins and neoglycoproteins combined with silver enhancement have been used for the detection of carbohydrates and CRD, respectively, on the SC of M. javanica J2. Biotin reagents were used to trace surface proteins, specifically, on live J2. The labile and transitory nature of the SC was demonstrated by the dynamics of HRBC adherence to detergent-treated J2, J2 at different ages or fresh-hatched J2 held at various temperatures. SC recovery was demonstrated also by a SDS-PAGE profile. Monoclonal antibodies developed to a cuticular protein of M. incognita J2 gave a slight, but significant reduction in attachment of Pasteuria penetrans spores. Spore attachment as affected by several enzymes was inconsistent: alcian blue, which specifically blocks sulfyl groups, had no afffect on spore attachment. Treatment with cationized ferritin alone or catonized ferritin following monoclonal antibody caused significant decreases in spore attachment. Those results suggest a role in attachment by negatively charged groups.
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Fahima, Tzion, and Jorge Dubcovsky. Map-based cloning of the novel stripe rust resistance gene YrG303 and its use to engineer 1B chromosome with multiple beneficial traits. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7598147.bard.

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Research problem: Bread wheat (Triticumaestivum) provides approximately 20% of the calories and proteins consumed by humankind. As the world population continues to increase, it is necessary to improve wheat yields, increase grain quality, and minimize the losses produced by biotic and abiotic stresses. Stripe rust, caused by Pucciniastriiformisf. sp. tritici(Pst), is one of the most destructive diseases of wheat. The new pathogen races are more virulent and aggressive than previous ones and have produced large economic losses. A rich source for stripe-rust resistance genes (Yr) was found in wild emmer wheat populations from Israel. Original Project goals: Our long term goal is to identify, map, clone, characterize and deploy in breeding, novel wild emmer Yr genes, and combine them with multiple beneficial traits. The current study was aiming to map and clone YrG303 and Yr15, located on chromosome 1BS and combine them with drought resistance and grain quality genes. Positional cloning of YrG303/Yr15: Fine mapping of these genes revealed that YrG303 is actually allelic to Yr15. Fine genetic mapping using large segregating populations resulted in reduction of the genetic interval spanning Yr15 to less than 0.1 cM. Physical mapping of the YrG303/Yr15 locus was based on the complete chromosome 1BS physical map of wheat constructed by our group. Screening of 1BS BAC library with Yr15 markers revealed a long BAC scaffold covering the target region. The screening of T. dicoccoidesaccession-specific BAC library with Yr15 markers resulted in direct landing on the target site. Sequencing of T. dicoccoidesBAC clones that cover the YrG303/Yr15 locus revealed a single candidate gene (CG) with conserved domains that may indicate a role in disease resistance response. Validation of the CG was carried out using EMS mutagenesis (loss-of- function approach). Sequencing of the CG in susceptible yr15/yrG303 plants revealed three independent mutants that harbour non-functional yr15/yrG303 alleles within the CG conserved domains, and therefore validated its function as a Pstresistance gene. Evaluation of marker-assisted-selection (MAS) for Yr15. Introgressions of Yr15 into cultivated wheat are widely used now. Recently, we have shown that DNA markers linked to Yr15 can be used as efficient tools for introgression of Yr15 into cultivated wheat via MAS. The developed markers were consistent and polymorphic in all 34 tested introgressions and are the most recommended markers for the introgression of Yr15. These markers will facilitate simultaneous selection for multiple Yr genes and help to avoid escapees during the selection process. Engineering of improved chromosome 1BS that harbors multiple beneficial traits. We have implemented the knowledge and genetic resources accumulated in this project for the engineering of 1B "super-chromosome" that harbors multiple beneficial traits. We completed the generation of a chromosome including the rye 1RS distal segment associated with improved drought tolerance with the Yr gene, Yr15, and the strong gluten allele 7Bx-over-expressor (7Bxᴼᴱ). We have completed the introgression of this improved chromosome into our recently released variety Patwin-515HP and our rain fed variety Kern, as well as to our top breeding lines UC1767 and UC1745. Elucidating the mechanism of resistance exhibited by Yr36 (WKS1). The WHEAT KINASE START1 (WKS1) resistance gene (Yr36) confers partial resistance to Pst. We have shown that wheat plants transformed with WKS1 transcript are resistant to Pst. WKS1 is targeted to the chloroplast where it phosphorylates the thylakoid-associatedascorbateperoxidase (tAPX) and reduces its ability to detoxify peroxides. Based on these results, we propose that the phosphorylation of tAPX by WKS1 reduces the ability of the cells to detoxify ROS and contributes to cell death. Distribution and diversity of WKS in wild emmer populations. We have shown that WKS1 is present only in the southern distribution range of wild emmer in the Fertile Crescent. Sequence analysis revealed a high level of WKS1 conservation among wild emmer populations, in contrast to the high level of diversity observed in NB-LRR genes. This phenomenon shed some light on the evolution of genes that confer partial resistance to Pst. Three new WKS1 haplotypes displayed a resistance response, suggesting that they can be useful to improve wheat resistance to Pst. In summary, we have improved our understanding of cereals’ resistance mechanisms to rusts and we have used that knowledge to develop improved wheat varieties.
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Alfano, James, Isaac Barash, Thomas Clemente, Paul E. Staswick, Guido Sessa, and Shulamit Manulis. Elucidating the Functions of Type III Effectors from Necrogenic and Tumorigenic Bacterial Pathogens. United States Department of Agriculture, January 2010. http://dx.doi.org/10.32747/2010.7592638.bard.

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Many phytopathogenic bacteria use a type III protein secretion system (T3SS) to inject type III effectors into plant cells. In the experiments supported by this one-year feasibility study we investigated type III effector function in plants by using two contrasting bacterial pathogens: Pseudomonas syringae pv. tomato, a necrotrophic pathogen and Pantoea agglomerans, a tumorigenic pathogen. The objectives are listed below along with our major conclusions, achievements, and implications for science and agriculture. Objective 1: Compare Pseudomonas syringae and Pantoea agglomerans type III effectors in established assays to test the extent that they can suppress innate immunity and incite tumorigenesis. We tested P. agglomerans type III effectors in several innate immunity suppression assays and in several instances these effectors were capable of suppressing plant immunity, outputs that are suppressed by P. syringae effectors. Interestingly, several P. syringae effectors were able to complement gall production to a P. agglomerans pthGmutant. These results suggest that even though the disease symptoms of these pathogens are dramatically different, their type III effectors may function similarly. Objective 2: Construct P. syringae mutants in different combinations of type III-related DNA clusters to reduce type III effector redundancy. To determine their involvement in pathogenicity we constructed mutants that lack individual and multiple type III-related DNA clusters using a Flprecombinase-mediated mutagenesis strategy. The majority of single effector mutants in DC3000 have weak pathogenicity phenotypes most likely due to functional redundancy of effectors. Supporting this idea, Poly-DNAcluster deletion mutants were more significantly reduced in their ability to cause disease. Because these mutants have less functional redundancy of type III effectors, they should help identify P. syringae and P. agglomerans effectors that contribute more significantly to virulence. Objective 3: Determine the extent that P. syringae and P. agglomerans type III effectors alter hormone levels in plants. Inhibition of auxin polar transport by 2,3,5-triiodobenzoic acid (TIBA) completely prevented gall formation by P. agglomerans pv. gypsophilae in gypsophila cuttings. This result supported the hypothesis that auxin and presumably cytokinins of plant origin, rather than the IAA and cytokinins secreted by the pathogen, are mandatory for gall formation. Transgenic tobacco with pthGshowed various phenotypic traits that suggest manipulation of auxin metabolism. Moreover, the auxin levels in pthGtransgenic tobacco lines was 2-4 times higher than the control plants. External addition of auxin or cytokinins could modify the gall size in gypsophila cuttings inoculated with pthGmutant (PagMx27), but not with other type III effectors. We are currently determining hormone levels in transgenic plants expressing different type III effectors. Objective 4: Determine whether the P. agglomerans effectors HsvG/B act as transcriptional activators in plants. The P. agglomerans type III effectors HsvG and HsvB localize to the nucleus of host and nonhost plants and act as transcription activators in yeast. Three sites of adjacent arginine and lysine in HsvG and HsvB were suspected to act as Nuclear localization signals (NLS) domains. A nuclear import assay indicated two of the three putative NLS domains were functional NLSs in yeast. These were shown to be active in plants by fusing HsvG and HsvB to YFP. localization to the nucleus was dependent on these NLS domains. These achievements indicate that our research plan is feasible and suggest that type III effectors suppress innate immunity and modulate plant hormones. This information has the potential to be exploited to improve disease resistance in agricultural crops.
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