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Walker, Lisa J. "HAP1 : a human DNA repair enzyme." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386682.
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Barzilay, Gil. "Characterisation of human AP endonuclease I (HAP1)." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318791.
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Wu, Linyan, and wu0071@flinders edu au. "BRAIN DERIVED NEUROTROPHIC FACTOR TRANSPORT AND PHYSIOLOGICAL SIGNIFICANCE." Flinders University. Medicine, 2007. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20071204.113001.
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Neurotrophins are important signaling molecules in neuronal survival and differentiation. The precursor forms of neurotrophins (proneurotrophins) are the dominant form of gene products in animals, which are cleaved to generate prodomain and mature neurotrophins, and are sorted to constitutive or regulated secretory pathway and released. Brain-derived neurotrophic factor (BDNF) plays a pivotal role in the brain development and in the pathogenesis of neurological diseases. In Huntingtons disease, the defective transport of BDNF in cortical and striatal neurons and the highly expressed polyQ mutant huntingtin (Htt) result in the degeneration of striatal neurons. The underlying mechanism of BDNF transport and release is remains to be investigated. Current studies were conducted to identify the mechanisms of how BDNF is transported in axons post Golgi trafficking. By using affinity purification and 2D-DIGE assay, we show Huntingtin-associated protein 1 (HAP1) interacts with the prodomain and mature BDNF. The GST pull-down assays have addressed that HAP1 directly binds to the prodomain but not to mature BDNF and this binding is decreased by PolyQ Htt. HAP1 immunoprecipitation shows that less proBDNF is associated with HAP1 in the brain homogenate of Huntingtons disease compared to the control. Co-transfections of HAP1 and BDNF plasmids in PC12 cells show HAP1 is colocalized with proBDNF and the prodomain, but not mature BDNF. ProBDNF was accumulated in the proximal and distal segments of crushed sciatic nerve in wild type mice but not in HAP1-/- mice. The activity-dependent release of the prodomain of BDNF is abolished in HAP1-/- mice. We conclude that HAP1 is the cargo-carrying molecule for proBDNF-containing vesicles and plays an essential role in the transport and release of BDNF in neuronal cells. 20-30% of people have a valine to methionine mutation at codon 66 (Val66Met) in the prodomain BDNF, which results in the retardation of transport and release of BDNF, but the mechanism is not known. Here, GST-pull down assays demonstrate that HAP1 binds Val66Met prodomain with less efficiency than the wild type and PolyQ Htt further reduced the binding, but the PC12 cells colocalization rate is almost the same between wt prodomain/HAP1 and Val66Met prodomain/HAP1, suggesting that the mutation in the prodomain may reduce the release by impairing the cargo-carrying efficiency of HAP1, but the mutation does not disrupt the sorting process. Recent studies have shown that proneurotrophins bind p75NTR and sortilin with high affinity, and trigger apoptosis of neurons in vitro. Here, we show that proBDNF plays a role in the death of axotomized sensory neurons. ProBDNF, p75NTR and sortilin are highly expressed in DRG neurons. The recombinant proBDNF induces the dose-dependent death of PC12 cells and the death activity is completely abolished in the presence of antibodies against the prodomain of BDNF. The exogenous proBDNF enhances the death of axotomized sensory neurons and the antibodies to the prodomain or exogenous sortilin-extracellular domain-Fc fusion molecule reduces the death of axotomized sensory neurons. We conclude that proBDNF induces the death of sensory neurons in neonatal rats and the suppression of endogenous proBDNF rescued the death of axotomized sensory neurons.
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Macêdo, Cláudia Souza Macedo. "Caracterização fisiológica de mutantes Kluyveromyces lactis ∆hap1 e ∆rox1 sob aerobiose e hipoxia." Universidade Federal de Viçosa, 2005. http://www.locus.ufv.br/handle/123456789/10694.
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Na busca de novos resultados para elucidar o papel de HAP1 e ROX1, que codificam um ativador do metabolismo oxidativo e um repressor do metabolismo oxidorredutivo, respectivamente, na levedura Crabtree negativa Kluyveromyces lactis cuja versatilidade metabólica pode ser explorada em vários campos da biotecnologia, inicialmente, foi identificado um gene ortólogo ROX1 à S. cerevisiae na seqüência genômica de K.lactis. O gene KlROX1 possui 40% de identidade daquele presente em S. cerevisiae e um motif característico do domínio HMG (High Mobility Group). Com base nessa seqüência uma linhagem mutante com deleção de ROX1 foi construída e confirmada. O fenótipo URA + e rox1 - dos transformantes obtidos, K.lactis ∆rox1::URA3, foram 100% estáveis sob condição seletiva. O gene putativo ROX1 de K.lactis teve a sua função em resposta ao oxigênio confirmada em culturas de K. lactis sob regime contínuo e desrepressão por glicose, pois, a deleção de ROX1 induziu a um aumento no nível do transcrito do gene hipóxico HEM13. A análise dos produtos do metabolismo permitiu inferir que a deleção do gene ROX1 em K. lactis aumentou a capacidade fermentativa dessa levedura sob aerobiose e de desrepressão catabólica. A investigação em culturas K. lactis ∆hap1 submetidas ao cultivo contínuo aeróbico sob desrepressão por glicose revelou um fenótipo relacionado ao metabolismo oxidorredutivo, ou seja, K. lactis ∆hap1 é mais fermentativa levando a diversidade de metabólitos em torno do piruvato. A proposta da via de regulação parcial negativa controlando a expressão de HEM13 foi confirmada nas culturas K. lactis.
The objective of this study was to search for new results in order to elucidate the role of HAP1 and ROX1, which codify an oxidative metabolism activator and an oxidoreductive metabolism repressor respectively, in the Crabtree-negative yeast Kluyveromyces lactis, whose metabolic versatility can be exploited in several biotechnology fields. Initially, a ROX1 gene orthologous to S. cerevisiae was identified in the genomic sequence of K. lactis. The KlROX1 gene has 40% identity with the one present in S. cerevisiae and a characteristic motif of the HMG (High Mobility Group) domain. Based on this sequence, a mutant line with ROX1 deletion was built and confirmed. The obtained transformant URA + and rox1 - phenotypes, K. lactis ∆rox1::URA3, were 100% stable under selective condition. The putative K. lactis ROX1 gene had its function in response to oxygen confirmed in cultures of K. lactis under continuous regime and glucose derepression, since ROX1 deletion induced an increase in the level of the HEM13 hypoxic gene transcript. The analysis of metabolism products allowed inferring that the deletion of gene ROX1 in K. lactis increased the yeast fermentative capacity under aerobic and catabolic derepression. The investigation in K. lactis ∆hap1 cultures under continuous aerobic cultivation and glucose derepressiom revealed a phenotype related to oxidoreductive metabolism, in other words, K. lactis ∆hap1 is more fermentative, leading to metabolite diversity around the piruvate. The proposal of the partial negative regulation pathway controlling the HEM13 expression was confirmed in K. lactis cultures.
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DEFRANOUX, NADINE. "Analyse moleculaire du gene cyp1 (hap1) codant pour un regulateur transcriptionnel multifonctionnel de saccharomyces cerevisiae." Paris 7, 1991. http://www.theses.fr/1991PA077157.
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Chez saccharomyces cerevisiae, le regulateur transcriptionnel cyp1 (ha1) module en fonction de l'etat d'oxydo-reduction de la cellule, l'expression de genes codant pour des apohemoproteines ou des enzymes liees a l'utilisation de l'oxygene. Suivant le gene cible et la presence ou l'absence d'heme ou d'oxygene, il se comporte comme un activateur ou comme un represseur. En presence d'heme et d'oxygene, cyp1 se fixe sur des sequences nucleotidiques qui ne presentent pas de consensus bien defini. Ce phenotype complexe pose la question de savoir s'il s'agit d'une proteine a zinc cluster d'autant qu'un residu histidine occupe la position invariable du sixieme residu cysteine indispensable a la formation de la structure. Des souches mutees au locus cyp1 presentant un double phenotype ont ete isolees au laboratoire. Les proteines mutantes discriminent deux sequences cibles peu similaires: l'une de ces cibles n'est plus reconnue alors que pour l'autre non seulement la fixation est preservee mais elle s'accompagne d'une superactivation du gene. Nous avons montre que ce phenotype pleiotrope resulte d'une substitution serine vers arginine (en position 63) situee a la base de la structure a zinc. La fonction de l'acide amine present a cette position a ete extensivement etudie. Nous avons introduit par mutagenese dirigee des mutations faux-sens soit a la position 63, soit au niveau des ligands possibles du zinc. Nos resultats montrent que: 1) cyp1 appartient a la famille des regulateurs a zinc cluster. Un residu cysteine bien que localise a une position inhabituelle par rapport a la sequence consensus, est le sixieme ligand du zinc; 2) la structure zinc cluster est impliquee tant dans la regulation negative que positive, que les cellules soient carencees en heme ou pas; 3) l'acide amine precedant la premiere cysteine du motif appartient a un element qui intervient dans la discrimination des cibles. De plus, la nature de cet acide amine affecte l'efficacite de l'activite regulatrice de cyp1
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Vieira, Nichelle Antunes. "Caracterização de células humanas Hap1 nocaute para FBXO25: via de sinalização da ERK quinase e proliferação celular." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/17/17131/tde-25042018-143544/.
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A proteína FBXO25 é uma E3-ligase do tipo SCF, responsável pela seletividade da ligação da Ub à proteína substrato e pelo direcionamento da proteína marcada para o barril proteassomal 26s. Sabe-se que FBXO25 é capaz de interagir e ubiquitinar a proteína Elk-1 em células HEK293T e, assim, inibir a expressão de genes importantes na regulação da proliferação celular, como C-FOS e EGR-1, após estímulo com o mitógeno PMA. Aqui mostramos que FBXO25 atua em um outro ponto da via das MAPKs, modulando os níveis de fosforilação de ERK1/2. Por meio da utilização de células nocaute para FBXO25 (FBXO25KO) foi possível observar que o tratamento com PMA promoveu aumento dos níveis de fosforilação de ERK1/2 nestas células quando comparadas com sua linhagem parental. Observouse também que o estímulo com os mitógenos PMA ou ATP levou a um aumento da proliferação celular não relacionada à modulação direta do ciclo celular nas células nocautes, sendo que estas apresentaram uma redução significativa dos seus níveis de apoptose. Tomando esses resultados em conjunto, mostramos que FBXO25 atua sobre a sinalização de MAPK por meio de redução da ativação ERK1/2 e, dessa forma, promove uma resposta secundária sobre o fenótipo de proliferação celularThe FBXO25 protein is an SCF-type E3-ligase responsible for the selectivity of Ub binding to the protein and the targeting of the labeled protein to the 26s proteasome barrel. FBXO25 has been long known to be able to interact and ubiquitinate the Elk-1 protein in HEK293T cells, thereby inducing a decrease in the expression of important genes in the regulation of cell proliferation such as CFOS and EGR-1 after stimulation with the mitogen PMA. Here we show that FBXO25 acts at another point in the MAPK pathway by modulating the ERK1/2 phosphorylation levels. We observed that the treatment with PMA rised the phosphorylated levels of ERK1/2 in knockout cells for FBXO25 (FBXO25KO) when compared to its parental lineage. Stimulation with the mitogens PMA or ATP also led to an increase in cell proliferation unrelated to a direct modulation of the cell cycle in knockout cells, with a significant weight of apoptosis levels being observed. Taking these results together, we show that FBXO25 acts on MAPK signaling by reducing ERK1/2 activation and thus promotes a secondary response on the cell proliferation phenotype.
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Capps, Denise. "The Regulation of NAP4 in Saccharomyces cerevisiae." ScholarWorks@UNO, 2011. http://scholarworks.uno.edu/td/116.
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The CCAAT binding-factor (CBF) is a transcriptional activator conserved in eukaryotes. The CBF in Saccharomyces cerevisiae is a multimeric heteromer termed the Hap2/3/4/5 complex. Hap4, which contains the activation domain of the complex, is also the regulatory subunit and is known to be transcriptionally controlled by carbon sources. However, little is known about Hap4 regulation. In this report, I identify mechanisms by which Hap4 is regulated, including: (1) transcriptional regulation via two short upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of Hap4 cellular localization which depends on (1) carbon sources, (2) abundance of Hap4 protein, and (3) presence or absence of mitochondrial DNA (mtDNA).
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Mejia, Luis Antonio. "Interaction Proteomics of Autism Spectrum Disorder- and Intellectual Disability-Associated Proteins Identifies a Novel Hap1-Tsc1 Signaling Link that Controls Neuronal mTORC1 Signaling and Pyramidal Neuron Morphogenesis." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:11190.
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Autism spectrum disorder (ASD) and intellectual disability (ID) are neurodevelopmental disorders of cognition that remain incompletely understood. Here, using a computation-assisted interaction proteomics approach in neural cells including primary neurons, we isolate high-confidence binding partners of proteins linked to ASD and ID. As part of these studies, we uncover the brain-enriched, coiled-coil domain protein huntingtin-associated protein 1 (Hap1) as a novel functional binding partner of the tuberous sclerosis complex (TSC) protein Tsc1. We validate and map the Hap1-Tsc1 interaction, and find that Hap1 and Tsc1 form a complex endogenously in the brain. Hap1 knockdown in primary hippocampal neurons triggers the specification of supernumerary axons, and in utero knockdown of Hap1 in mice profoundly impairs the positioning of pyramidal neurons in the hippocampus in vivo. Importantly, the Hap1 knockdown-induced phenotypes in primary neurons and in vivo recapitulate the phenotypes induced by Tsc1 knockdown. We also define a mechanism by which Hap1 regulates Tsc1 function. We observed that exogenous Hap1 promotes the abundance of soluble, stable Tsc1 expressed in cells. Hap1 knockdown in neurons reduces Tsc1 abundance and accordingly stimulates the activity of mTORC1, as reflected by phosphorylation of the ribosomal protein S6. Importantly, inhibition of mTORC1 signaling suppresses the Hap1 knockdown-induced axon phenotype in hippocampal neurons. Collectively, these findings define a novel relationship between Hap1 and Tsc1 that regulates neuronal Tsc1 abundance, pyramidal neuron development, and neuronal mTORC1 signaling, with important mechanistic implications for our understanding of neurodevelopmental disorders of cognition.
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Hunter, Arielle Ruth. "Characterization of Ubiquitin/Proteasome-Dependent Regulation of Hap2/3/4/5 Complex In Saccharomyces cerevisiae." ScholarWorks@UNO, 2012. http://scholarworks.uno.edu/honors_theses/13.
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The Hap2/3/4/5 complex is a heme-activated, CCAATT binding, global transcriptional activator of genes involved in respiration and mitochondrial biogenesis in the yeast species Saccharomyces cerevisiae. Hap4 is the regulatory subunit of the complex and its levelsdetermine the activity of the complex. Hap4 is known to play a signaling role in response toenvironmental conditions; however, little is known about the regulation of Hap4 levels or how it responses to a cell’s functional state. The activity of the Hap2-5 complex is known to be reduced in respiratory-deficient cells. In Liu Lab, it has previously been found that a link between Hap4 stability, mediated through 26S proteasome-dependent degradation, and dependence on mitochondrial functional state plays a regulatory role on downstream targets of the Hap complex. However, the mechanism behind this regulation is still largely unknown. In normally functioning yeast cells, Hap4 is a highly unstable protein with a half-life of ~10 min. We have observed that loss of mitochondrial DNA in respiratory deficient rho 0 cells has a role in the further destabilization of Hap4 to a half-life of ~4 min through the ubiquitin-proteasome pathway. Through the screening of a collection of mutants defective in E2 ubiquitin-conjugating enzymes, we show that Hap4 is greatly stabilized in ubc1Δubc4Δ double mutant cells. We also show that Hap4 stabilization in the ubc1Δubc4Δ mutant leads to increased activity of the Hap2-5 complex, indicating that mitochondrial biogenesis in yeast is regulated by the functional state of mitochondria through ubiquitin/proteasome-dependent degradation of Hap4. Furthermore, studies on Hap4 mutants involving two highly conserved cysteine residues led to a proposed mechanism behind the regulation of Ubc4 activity towards Hap4 in response to changes in the cellular redox state.
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Higgins, Robert Francis 1962. "Hercules attitude processor (HAP)." Diss., Virginia Tech, 1992. http://hdl.handle.net/10919/38349.
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The design and analysis of a microprocessor-based gyro attitude data processing system used to geolocate natural phenomena from space was performed. Operational software was written and a HERCULES Attitude Processor (HAP) unit was built and tested.
Strict adherence to worst-case timing design criterion was a prime hardware design consideration. Weight, volume, and power requirements were also addressed. Redundancy was included for critical time maintenance functions. Hardware performance and accuracy was calculated and measured.
Operational software was written to control the functions of the HAP unit. Algorithms were written to accurately process the high speed gyro attitude data. Data communication between subsystems in the HERCULES system was controlled by the software. Subsystem configuration, operating modes, self-testing, and resource management was performed by the operational software.
Testing was performed on the HAP unit and operational software. Hardware and software performance was analyzed and is presented.Ph. D.
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Happ, Peter [Verfasser]. "Heterometallacrowns as high-spin molecules / Peter Happ." Mainz : Universitätsbibliothek Mainz, 2016. http://d-nb.info/1111056099/34.
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Happ, Carolin [Verfasser]. "Internationaler Rechtsschutz gegen fehlerhafte Ratings / Carolin Happ." Frankfurt : Peter Lang GmbH, Internationaler Verlag der Wissenschaften, 2015. http://d-nb.info/1080460713/34.
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Le, Guillou Ian. "Studies of the human histone acetyltransferase HAT1." Thesis, University of Cambridge, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648729.
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Appaswamy, Giridharan [Verfasser]. "The role of HAX1 in neutrophil homeostasis / Giridharan Appaswamy." Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2011. http://d-nb.info/1013473183/34.
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Cisbani, Giulia. "PARL et HAX1 dans la régulation de l'activité mitochondriale." Thesis, Université Laval, 2010. http://www.theses.ulaval.ca/2010/26847/26847.pdf.
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Williams, Daniel Benjamin. "Hap: Uncertainty and the English Novel." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:17467238.
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This dissertation explores how nineteenth-century novelists envisioned thinking, judging, and acting in conditions of imperfect knowledge. I place novels against historical developments in mathematics, philosophy, psychology, and jurisprudence to argue that William Thackeray, George Eliot, Wilkie Collins, and Thomas Hardy generated distinctive aesthetic and affective responses to uncertainty. I anchor these novelists in nineteenth-century intellectual contexts with which they were familiar, including the transition from associationism to an embodied picture of psychology and motivation; the rise of statistical thinking and calculative rationality; the renewal of inductive methods in the sciences; and approaches to probability as a concept whose various senses converge. I spotlight how novels interact with cultural domains of uncertain knowledge, from gambling to weather forecasting to legal decision. Articulating a phenomenology of uncertainty that is shaped by, yet often resistant to, the nascent sciences of prediction and calculation in the period, novels attend to the felt effects, aesthetic repercussions, and emotional tonality of judging and acting without certain knowledge. I argue that they refract their environing contexts with striking consequences for narrative form, aesthetic theory, and generic commitment. And I claim that they deepen their approaches to scientific knowledge and social concern with a focus on what uncertainty looks and feels like as a subjective experience: on speculations that run against the grain of fact (Thackeray); hesitations that almost entirely usurp action (Eliot); legal judgments and verdicts that lack finality and proof (Collins); and forms of repetition and aggregation that we use in everyday inference (Hardy). Affective dimensions of uncertainty mediate between the scales of concept and experience: Thackeray’s counterfactual imaginary probes the emotional tone of speculations about alternative realities; Eliot’s interest in theories of decision meets with hesitation as a practical attitude and bodily experience; Collins’ exploration of legal uncertainty is shadowed by the psychology of suspicion; and Hardy’s deployment of logical and statistical models consorts with sensation and intuition. Throughout I draw connections between these styles of uncertain thinking and literary reading, offering updated accounts of inference, evidence, and especially probability—as numerical concept, epistemic conundrum, legal tool, and rhetorical protocol.English
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Rabodonirina, Rondro Suzanah. "Contaminants organiques (HAP, Me-HAP, PCB) en environnement : étude de milieu naturel et de faisabilité de bioremédiation." Thesis, Lille 1, 2015. http://www.theses.fr/2015LIL10170/document.
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La révolution industrielle, l’évolution technologique, la croissance démographique et l’urbanisation ont affecté non seulement l’économie ou la politique dans le monde mais également la santé environnementale. Divers types de polluants sont générés et introduits, de manière permanente, dans l’environnement via les effluents industriels, agricoles et/ou municipaux. Parmi ces polluants figurent les polluants organiques persistants ou POP qui sont susceptibles de développer une toxicité non négligeable pour la santé humaine et le bon fonctionnement de l’écosystème. En plus, ils sont détectés dans tous les composants de l’écosystème global, y compris l’atmosphère, les ressources en eaux, le sol, les sédiments et les biotes. Depuis la découverte des insecticides DDT dans les tissus humains, le sort et le comportement de ces polluants dangereux ont retenu l’attention de la recherche scientifique. Dans le présent travail, on s’intéresse essentiellement aux POP du type hydrocarbures aromatiques polycycliques (HAP) et ses dérivés méthylés (Me-HAP) ainsi que les polychlorobiphényles (PCB). La première partie de l’étude est consacré à l’évaluation de leur niveau de contamination dans différents compartiments de l’environnement. Leur occurrence et distribution ont été évaluées dans les phases dissoutes, particulaires et sédimentaires du système d’eaux douces du bassin versant de l'Escaut dans la zone transfrontalière France-Belgique dans le cadre du projet FRB-RégionNPC "BIOFOZI" suivi d’une étude écotoxicologique. La même investigation est réalisée sur des sols originaires du Nord-Pas-de Calais (France) et de Madagascar. La seconde partie contribue à une étude de faisabilité de traitement biologique de sols contaminés par les HAPThe industrial revolution, technological change, population growth and urbanization have affected not only the economy and politics all around the world but also the environment. Various types of pollutants are generated and introduced permanently into the environment through industrial, agricultural and/or municipal discharges. These pollutants include the persistent organic pollutants or POPs which can develop dangerous effects to human health and the ecosystem. In addition, they are reported detected in all the environmental components including the atmosphere, water resources, soils, sediments and biota. Since the discovery of the insecticides DDT in human tissue, the fate and behavior of these type of hazardous pollutants have caught the interest of researchers. In this work, our target contaminants are polycyclic aromatic hydrocarbons (PAHs) and their methylated derivatives (Me-PAHs) and polychlorinated biphenyls (PCBs). The first part of our study investigated the contamination level of theses POPs in the different compartments of the environment (water, suspended solid matters, sediment, soil). Their occurrence and distribution were evaluated in the dissolved, particulate and sedimentary phases of the freshwater system of the Scheldt basin in the border area between France and Belgium in FRB-RegionNPC "BIOFOZI" program. An ecotoxicological risk assessment was also carried out. The same investigations were also conducted in soil samples originating from northern France and from Madagascar. The second part of the present work concern a feasibility study of PAHs-contaminated soil bio-treatment (bioremediation)
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Bulagonda, Eswarappa Pradeep [Verfasser]. "Identification and characterization of HAX1 interacting proteins / Eswarappa Pradeep Bulagonda." Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2013. http://d-nb.info/1034052918/34.
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Lares, Monica Rae Jung. "Insights into the function of a novel RNA gene : HAR1 /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2009. http://uclibs.org/PID/11984.
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Bürger, Thomas. "HAP Grieshabers Andrucke zum Basler/Dresdner Totentanz." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2007. http://nbn-resolving.de/urn:nbn:de:swb:14-1192536265358-50176.
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Mit den von HAP Grieshaber geliebten Carmina Burana und einer Multimediapräsentation, gestaltet von Martin Kaufmann, begann am 19. Juli die Eröffnung der Ausstellung von Andrucken HAP Grieshabers zu seiner Dresdner Ausgabe des Basler Totentanzes (20. Juli bis 29. September 2007).
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Kopuletá, Ema. "Struktura a vlastnosti nanokompozitních sítí kolagen/HAP." Doctoral thesis, Vysoké učení technické v Brně. Fakulta chemická, 2014. http://www.nusl.cz/ntk/nusl-233390.
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Polymerní biomateriály jsou jedním ze současných populárních témat vzhledem k možnosti potenciální aplikace v tkáňovém inženýrství a řízeného dávkování léčiv v organismech. Kolagen je jako jeden z nejčastěji se vyskytujících proteinů zvláště zajímavý díky svým rozmanitým vlastnostem bez imunoreakce organismu příjemce. Tato práce je zaměřena na samouspořádávací procesy, kinetiku, obecné zákonitosti řídící proces samouspořádání a mechanické vlastnosti kolagenních roztoků. Dále je zkoumán efekt hydroxyapatitových nanočástic na samouspořádávání kolagenu a mechanické vlastnosti výsledných nanokompozitních hydrogelů. Jsou objasněny možné mechanismy interakcí mezi kolagenem I a hydroxyapatitem spolu s popisem vývoje struktury a vlastností na různých úrovních struktury. Byly měřeny a molekulárně interpretovány závislosti viskoelastických veličin na smykové rychlosti spolu s viskoelastickým chováním. Dále byla studována struktura kolagenních scaffoldů a určen vliv HAP a síťování. Závěrem byly diskutovány výsledky v souvislosti s jejich aplikovatelností v tkáňovém inženýrství chrupavek tvrdých tkání a v regenerativní medicíně.
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Valois, Fabricio Martins [UNIFESP]. "Contrastes entre as respostas fisiológicas ao exercício de pacientes com hipertensão arterial pulmonar (HAP) associada à esquistossomose e HAP idiopática." Universidade Federal de São Paulo (UNIFESP), 2012. http://repositorio.unifesp.br/handle/11600/22224.
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Previous issue date: 2012Introdução: A esquistossomose e potencialmente a causa mais comum de hipertensao arterial pulmonar (HAP) no mundo. Apesar de semelhancas histopatologicas com a HAP idiopatica (HAPI), a HAP associada a esquistossomose (HAP-Esq) apresenta um curso clinico mais benigno, desconhecendo-se, no entanto, os mecanismos que justificam esse disparate. Como a HAP e uma doenca que manifesta-se prioritariamente aos esforcos, a hipotese central deste trabalho foi a de que pacientes com HAP-Esq apresentem respostas fisiologicas ao exercicio incremental, avaliadas atraves de teste de exercicio cardiopulmonar (TECP) em cicloergometro, menos comprometidas que pacientes com HAPI. Objetivos: Primariamente, objetivou-se contrastar as diferencas nos ajustes fisiologicos durante o exercicio dinamico entre pacientes com HAPI e HAP-Esq; secundariamente, objetivou-se correlacionar as variaveis hemodinamicas de repouso com os parametros do TECP e, ainda, avaliar a capacidade do teste de caminhada de seis minutos (TC6M) em identificar limitacoes no desempenho aerobico dos pacientes com HAPI e HAP-Esq. Metodos: Foram analisados TECP incremental em cicloergometro e TC6M de pacientes com HAPI e HAP-Esq, realizados ate 1 mes apos a avaliacao hemodinamica diagnostica, e sem que tratamento especifico para HAP tivesse sido iniciado. Houve duas fases distintas no delineamento do estudo: i) analise retrospectiva de pacientes que realizaram TECP como parte da rotina ambulatorial ou de protocolos de pesquisa; ii) fase prospectiva, na qual individuos com diagnostico recente de HAPI ou HAP-Esq foram submetidos a TECP incremental. Resultados: Foram avaliados 8 pacientes com HAP-Esq e 9 com HAPI. O indice cardiaco (IC), a resistencia vascular pulmonar e a pressao media de arteria pulmonar foram similares entre os grupos. Nao houve diferencas na estimativa da classe funcional da New York Heart Association e na distancia caminhada no TC6M entre os grupos. No entanto, no TECP, o O2pico foi maior em pacientes com HAP-Esq (75,5 ± 21,4 vs 54,1 ± 16,1 %previsto; p=0,016), assim como a inclinacao da relacao O2-W (8,2 ± 1,0 vs 6,8 ± 1,8 mL/min/W; p=0,03). Os individuos com HAP-Esq apresentaram maiores valores de pulso de oxigenio comparativamente a HAPI, alem de menor resposta taquicardica conforme a demanda metabolica (80,1 ± 20,6 vs 123,0 ± 39,2 batimentos/L/min; p=0,02). A inclinacao da relacao E- CO2 foi menor na HAPEsq (40,3 ± 3,9 vs 55,6 ± 19,8; p= 0,04). Houve correlacao entre o IC de repouso e O2pico, O2/W e E/ CO2 apenas nos pacientes com HAPI. Nao houve correlacao do O2pico com a distancia caminhada no TC6M. Conclusoes: Pacientes com HAPEsq apresentaram respostas fisiologicas ao exercicio incremental menos comprometidas que os pacientes com HAPI, mesmo com padrao hemodinamico similar ao repouso. Houve correlacao entre variaveis do TECP e hemodinamicas de repouso apenas nos pacientes com HAPI. O TC6M nao apresentou capacidade de detectar as disparidades funcionais entre os grupos. Os achados ao TECP sugerem que os pacientes com HAP-Esq possuam melhor capacidade em ajustar o debito cardiaco conforme a demanda metabolica durante o esforco, o que pode explicar o melhor curso clinico observado comparativamente a HAPI
BV UNIFESP: Teses e dissertações
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Mazeas, Olivier. "Evaluation de l'exposition des organismes aux hydrocarbures aromatiques polycycliques (HAP) dans le milieu marin par le dosage des métabolites de HAP." Bordeaux 1, 2004. http://www.theses.fr/2004BOR12945.
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Afin d'étudier la santé d'un écosystème marin et le potentiel toxique d'une contamination telle que celle liée à la présence d'hydrocarbures aromatiques polycycliques (HAP), il est nécessaire, outre de connaître les niveaux de contamination du milieu, de pouvoir accéder à la fraction à laquelle les organismes aquatiques ont été exposés et de connaître les effets toxiques des contaminants incriminés (effets toxiques qui peuvent être reliés aux phénomènes de biotransformation des contaminants). L'exposition et la contamination des organismes aquatiques aux HAP ont généralement été évaluées par le dosage des HAP bioaccumulés dans les tissus. Or, cette approche est critiquable si l'on tient compte des capacités de biotransformation des organismes, notamment des vertébrés, et des propriétés toxiques des produits de transformation formés. Dans ce contexte, l'objectif de cette thèse est d'évaluer le potentiel des métabolites de HAP dans le but d'étudier l'exposition et la contamination des organismes aux HAP dans l'environnement marin. Une méthode de dosage des métabolites de HAP dans les matrices biologiques a tout d'abord été mise au point. Ensuite, cet outil analytique a été appliqué lors d'expositions de poissons à des HAP individuels et à différents produits pétroliers en milieu contrôlé qui ont permis de mieux appréhender les phénomènes de biotransformation des HAP. Enfin, des études de terrain ont été réalisées, notamment dans le cadre du suivi de la marée noire Erika et de l'étude de la contamination de la baie de Seine, montrant l'applicabilité du dosage des métabolites de HAP afin d'évaluer l'exposition et la contamination des organismes aux HAP en milieu naturel dans des contextes de pollution de nature différenteIn order to study the health of a marine ecosystem and the toxic potential of a contamination such as the one related to the presence of polycyclic aromatic hydrocarbons (PAHs), it is necessary, in addition to the determination of environmental contamination levels, to have access to the fraction aquatic organisms have been exposed to and to identify the toxic effects of the contaminants (toxic effects which can be related to biotransformation phenomena of contaminants). The exposure and contamination of aquatic organisms to PAHs have generally been evaluated by the quantification of bioaccumulated PAHs in tissues. However, this approach is open to criticism when taking into account the biotransformation capabilities of organisms such as vertebrates and the toxic properties of biotransformation products. In this way, the aim of this study is to evaluate the potential of PAH metabolites in order to study the exposure and contamination of organisms to PAHs in the marine environment. An analytical technique to quantify PAH metabolites in biological matrices has first been set up. Then, this analytical tool has been applied to the study of fish exposures to individual PAHs and to different petroleum products in controlled conditions which has allowed to get a better understanding of PAH biotransformation phenomena. Finally, field studies have been led, notably to study the Erika oil spill and the contamination of the Seine bay, demonstrating the applicability of the quantification of PAH metabolites to evaluate the exposure and the contamination of organisms to PAHs in natural environment and for pollution events of different nature
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Happ, Christof J. [Verfasser]. "Adaptive and Non-Adaptive Estimation of Discrete Quantum States / Christof J. Happ." München : Verlag Dr. Hut, 2013. http://d-nb.info/104287820X/34.
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Happ, Christian [Verfasser], and Ulrich [Akademischer Betreuer] Wagner. "Empathy in Video Games and Other Media / Christian Happ. Betreuer: Ulrich Wagner." Marburg : Philipps-Universität Marburg, 2013. http://d-nb.info/1042276080/34.
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Goutebroze, Laurence. "Définition des bases moléculaires du pouvoir oncogène du polyomavirus de hamster (HaPV)." Paris 11, 1992. http://www.theses.fr/1992PA11T019.
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Verrhiest, Ghislaine. "Toxicité de sédiments d'eau douce contaminés par des hydrocarbures aromatiques polycycliques (HAPs)." Chambéry, 2001. http://www.theses.fr/2001CHAMS018.
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Del, Aguila López Carlos Isaid. "Hidrocarburos aromáticos polinucleares (HAPs) en material particulado de la ciudad de Lima." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2018. https://hdl.handle.net/20.500.12672/10136.
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Determina un grupo particular de compuestos, los hidrocarburos aromáticos policíclicos conocidos tambien como hidrocarburos aromáticos polinucleares (HAPs o PAHs) en el aire ambiente de Lima. Se sabe que el origen de tales compuestos está relacionado con los procesos de combustión de la materia orgánica. Debido al escaso desarrollo industrial del país, se puede afirmar que, en el caso de Lima, tales compuestos provienen principalmente de las emisiones de los automóviles, esto es, el uso de combustibles fósiles. Las muestras, colectadas inicialmente para determinar partículas menores a 10um (PM10) tomadas en varios distritos de la ciudad, fueron luego extraídas con solvente, concentradas, purificadas y analizadas por cromatografía de gases con espectrometría de masas. A pesar de las conocidas limitaciones de determinar HAPs en filtros PM10, debido al que el filtro no puede retener los HAPs más livianos (los cuales pueden dar cuenta de más del 50% del total), se detectaron los HAPs más pesados, que se adsorben casi completamente al material particulado, entre ellos el más riesgoso, el Benz(a)pireno, un compuesto cancerígeno, cuya concentración llegó hasta 1.4 ng/m3 , encontrándose además, Criseno Benzo(b)fluoranteno, Benzo(k)fluoranteno, Indeno(1,2,3-cd)pireno y Benzo(g,h,i)perileno. Adicionalmente se calculó los cocientes de diagnóstico para algunos HAPs, hallándose que el cociente Indeno(1,2,3-cd)pireno / [Indeno(1,2,3-cd)pireno+Benzo(g,h,i)perileno] se encontraba entre 0.39 y 0.5 para la mayoría de los puntos de muestreo lo cual encaja bastante bien con el valor del cociente para diésel de 0.38 a 0.64 reportado en la bibliografia, así mismo, el cociente Benzo(a)pireno / [Benzo(a)pireno+Criseno] que osciló entre 0.57 y 0.73 es coherente con el cociente para diésel de 0.5 y para gasolina 0.73, estos hallazgos refuerzan la hipótesis que estos compuestos han sido generados por el parque automotor de la ciudad. Resulta claro que las investigaciones sobre estos compuestos deberían ser ampliadas para cubrir más distritos de la ciudad, tanto en invierno como en verano para el estudio del comportamiento anual, y además de ello, colectar el material particulado y la fase gaseosa de modo que se conozca la magnitud real del problema en el contexto de la contaminación del aire de Lima. En ese sentido, la presente investigación contribuye al conocimiento público y revela la presencia de este grupo particular de compuestos nocivos para la salud, a los cuales estamos expuestos constantemente en las zonas con alto tránsito vehicular.Tesis
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Cassiani, Pamela Jean. "Investigating High Copy Suppressors of hat1∆ and rad52∆ Mutations in Fission Yeast." Thesis, Boston College, 2014. http://hdl.handle.net/2345/bc-ir:104173.
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Thesis advisor: Anthony T. AnnunziatoThe histone acetyltransferase Hat1 is an enzyme that specifically acetylates newly synthesized histone H4 at positions K5 and K12 (or their homologous positions) in all eukaryotes. In Schizosaccharomyces pombe, the deletion of hat1 presents a mutant phenotype. The telomeres in a hat1-del strain become permissive for transcription, as analyzed by a telomeric ura4 marker gene. In this study, we evaluate the efficacy of high copy suppression of this hat1 deletion. Due to high-frequency recombination events in the telomere, it became necessary to create a hat1-rad52 double deletion strain that also contains a telomeric ura4 reporter. High copy suppressor screens for recovery of telomeric silencing yielded several promising transformants. Multiple rounds of testing were performed to assess the recovery of transcriptional repression at the telomere. It was found that despite the anti-recombination effect of deleting rad52, the ura4 reporter was still lost from the telomere through recombination. Additional observation of the hat1-del rad52-del ura4-tel strain revealed a significant synthetic slow-growth phenotype. The double mutant displays a greatly decreased growth rate compared to hat1-del, as well as increased cellular length. Further study showed unique phenotypes on various media, and gene expression studies showed unique patterns of regulation in this double mutant when compared to both a wild- type and its single mutant counterparts (hat1-del, rad52-del). In summary, the telomeric ura4 marker in a hat1-del strain of S. pombe is not stable and is lost by recombination at a high frequency. This has led to the discovery of a double mutant (hat1-del rad52-del) that displays a severe synthetically sick phenotype
Thesis (MS) — Boston College, 2014
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Biology
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Tong, Kevin. "Characterization of the Schizosaccharomyces Pombe Hat1 Complex: the Role of Histone H4 Acetylation in Telomeric Silencing." Thesis, Boston College, 2009. http://hdl.handle.net/2345/2222.
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Thesis advisor: Anthony T. AnnunziatoThesis advisor: Charles Hoffman
The Hat1 complex was characterized in S. pombe. Through tandem affinity purification and mass spectrometry, it was determined that Hat1 is associated with Mis16 (an orthologue of HAT2). Unlike HAT2 in S. cerevisiae, we confirm mis16 to be an essential gene in S. pombe. As expected, the S. pombe Hat1 complex was found to acetylate lysines 5 and 12 of histone H4. In contrast to budding yeast, deletion of hat1 alone resulted in the loss of telomeric silencing without concomitant mutations of the H3 N-terminal domain. Deletion of hat1 caused an increase of H4 acetylation at telomeres. Additionally, the hyperacetylation of histones also results in the loss of telomeric silencing. Loss of Hat1 did not affect silencing at the inner most repeat (imr) or outer repeat (otr) regions of the centromere, but did appear to increase silencing at the central core region (cnt) of the centromere. The experiments described herein demonstrate Hat1 to be essential for the establishment of proper telomeric silencing in fission yeast, and suggest that the timely acetylation of H4 during chromatin assembly is a unique factor in generating the correct epigenetic state at telomeres in S. pombe. Additionally, Hat1 and its acetylation of new H4 may have entirely different roles during telomeric silencing than during silencing at the centromeric central core. Our studies in HeLa cells demonstrated that transcription is involved in the exchange of H2A/H2B in acetylated chromatin regions. The finding that cytosolic H2A can be acetylated at lysine 5 is the first demonstration that cytosolic H2A can be specifically modified in vivo. Our results support a model in which H2A/H2B exchange during transcription is mediated by the NAP1 chaperone
Thesis (PhD) — Boston College, 2009
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Biology
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Parish, Amy L. "Farmer willingness to adopt High Available Phosphorus (HAP) corn." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 145 p, 2008. http://proquest.umi.com/pqdweb?did=1459910291&sid=2&Fmt=2&clientId=8331&RQT=309&VName=PQD.
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Rutz, Kathrin Stoller Thomas. "Der dipl. Psych. FH/HAP zwischen Ausbildung und Marktanforderungen /." Zürich : Hochschule für Angewandte Psychologie, 2003. http://www.hapzh.ch/pdf/2s/2s0757.pdf.
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Cabral, Cynthia Hatsue Kitayama. "Determina??o de hapl?tipos do gene ?s em portadores de anemia falciforme." Universidade Federal do Rio Grande do Norte, 2010. http://repositorio.ufrn.br:8080/jspui/handle/123456789/18543.
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Previous issue date: 2010-05-11Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico
Haplotypes linked to the ?S gene represent patterns of DNA polymorphisms along chromosome 11 of individuals bearing the ?S gene. Analysis of haplotypes, in addition to serving as an important source for anthropological studies about the ethnic origin of a population, contributes to a better understanding of the variations in clinical severity of sickle cell anemia. The aim of the present study was to determine ?S gene haplotypes in a group of patients with sickle cell anemia treated at the Dalton Barbosa Cunha Hematology Center (Hemonorte) in Natal, Brazil and the Oncology and Hematology Center in Mossor?, Brazil. Blood samples were obtained from 53 non-related patients (27 males and 26 females), aged between 3 months and 61 years (mean age: 16.9 ? 12.1 years). Laboratory analyses consisted of the following: erythrogram, reticulocyte count, hemoglobin electrophoresis at alkaline pH, measurement of hemoglobin A2 and Fetal hemoglobin, solubility test and molecular analysis to determine ?S gene haplotypes. DNA samples were extracted by illustra blood genomicPrep Mini Spin kit and ?S gene haplotypes were determined by PCR-RFLP, using Xmn I, Hind III, Hinc II and Hinf I restriction enzymes for analysis of six polymorphic restriction sites in the beta cluster. Of 106 ?S chromosomes studied, 75.5% were Central African Republic (CAR) haplotype, 11.3% Benin (BEN) and 6.6% Cameroon (CAM). The atypical haplotypes had a frequency of 6.6%. More than half the patients (58.5%) were identified as CAR/CAR genotype carriers, 16.9% heterozygous CAR/BEN, 13.2% CAR/CAM and 1.9% BEN/BEN. Patients with atypical haplotype in one or two chromosomes accounted for 9.5% (CAR/Atp, BEN/Atp and Atp/Atp). The genotype groups showed no statistically significant difference (p < 0.05) in their laboratory parameters. This is the first study related to ?S haplotypes conducted in state of Rio Grande do Norte and the higher frequency of Cameroon halotype found, compared to other Brazilian states, suggests the existence of a peculiarity of African origin
Os hapl?tipos do gene ?s representam padr?es de polimorfismos do DNA ao longo do cromossomo 11 de indiv?duos portadores do gene ?s. A an?lise dos hapl?tipos, al?m de servir como uma fonte importante para estudos antropol?gicos acerca da origem ?tnica de uma popula??o, colabora para uma melhor compreens?o da varia??o de gravidade cl?nica da anemia falciforme. O presente estudo teve como objetivo determinar os hapl?tipos do gene ?s em um grupo de pacientes portadores de anemia falciforme atendidos no Ambulat?rio de Hematologia do Hemocentro Dalton Barbosa Cunha (Hemonorte) - Natal/RN e no Centro de Oncologia e Hematologia de Mossor?/RN. Foram obtidas amostras sang??neas de 53 pacientes n?o aparentados (27 do sexo masculino e 26 do sexo feminino), com idade variando entre 3 meses e 61 anos (m?dia de idade: 16,9 ? 12,1 anos). As an?lises laboratoriais consistiram em: eritrograma, contagem de reticul?citos, eletroforese de hemoglobina em pH alcalino, dosagem de hemoglobinas A2 e Fetal, teste de solubilidade e an?lise molecular para determin??o dos hapl?tipos ?s. O DNA foi extra?do pelo kit illustra blood genomicPrep Mini Spin e a determina??o dos hapl?tipos do gene S foi realizada por PCR-RFLP, utilizando as endonucleases de restri??o Xmn I, Hind III, Hinc II e Hinf I para an?lise de 6 s?tios polim?rficos do cluster ?. Os resultados observados mostraram, em rela??o ao n?mero de cromossomos, o predom?nio do hapl?tipo CAR (75,5%), frente aos hapl?tipos Benin (11,3%), e Camar?es (6,6%). Os hapl?tipos at?picos tiveram freq??ncia de 6,6%. Com rela??o aos gen?tipos, mais da metade dos pacientes (58,5%) foram identificados como portadores do gen?tipo CAR/CAR. O segundo mais freq?ente foi o CAR/BEN (16,9%), seguido de CAR/CAM (13,2%) e BEN/BEN (1,9%). Os pacientes com hapl?tipo at?pico em um ou nos dois cromossomos representaram 9,5% (CAR/Atp, BEN/Atp e Atp/Atp). N?o houve diferen?a estatisticamente significativa das m?dias dos par?metros laboratoriais entre os diferentes grupos de gen?tipos comparados. O presente trabalho ? o primeiro estudo relacionado aos hapl?tipos ?s realizado no estado do Rio Grande do Norte e o encontro de uma maior freq??ncia do hapl?tipo Camar?es, em rela??o a outros estados do Brasil, sugere a exist?ncia de uma peculiaridade da origem africana no estado
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Pech, Catherine. "Montage et qualification de l'expérience PIRENEA, photophysique des HAPs interstellaires : simulations et expériences." Toulouse 3, 2001. http://www.theses.fr/2001TOU30181.
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Collins, Morgan. "Negative Regulation of Haa1 by Casein Kinase I protein Hrr25 in Saccharomyces cerevisiae." ScholarWorks@UNO, 2017. http://scholarworks.uno.edu/td/2307.
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Haa1 is a transcription factor that adapts Saccharomyces cerevisiae cells to weak organic acid stresses by activating the expression of various genes. How Haa1 is activated by weak acids is not clear. This study proposes that Hrr25 is an important regulator of cellular adaptation to weak acid stress by inhibiting Haa1 through phosphorylation. YRO2, one of the targets of Haa1, shows increase in expression during stationary phase. This increase is due to basal activity of Haa1 and another, unknown, transcription factor. This study proposes that Gsm1 is another transcription factor that regulates YRO2 expression in the stationary phase. Finally, the mechanism of regulation of YRO2 by Haa1 is largely unknown. This study identifies two possible Haa1-medated cis-acting elements in the YRO2 promoter.
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Arcangioli, Benoit. "Etude de la regulation de la transcription chez la levure saccharomyces cerevisiae : exemple du gene de l'iso-1-cytochrome c." Paris 7, 1988. http://www.theses.fr/1988PA077004.
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Temelkuran, Baris 1980. "Hap-Shu : a language for locating information in HTML documents." Thesis, Massachusetts Institute of Technology, 2003. http://hdl.handle.net/1721.1/87882.
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Garon, David. "Dégradation fongique du fluorène (HAP) : optimisation et application aux sols." Université Joseph Fourier (Grenoble), 2002. http://www.theses.fr/2002GRE18002.
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L'utilisation de micromycètes dans la dépollution de sols contaminés par des hydrocarbures aromatiques polycycliques (HAP) constitue une alternative aux méthodes classiques, ces dernières présentant des inconvénients de faisabilité technique, de coût et d'efficacité. La bioremédiation paraît donc prometteuse même si la toxicité et le manque de biodisponibilité des polluants vis à vis des microorganismes capables de les dégrader sont deux obstacles à surmonter. Ce travail, utilisant le fluorène comme HAP modèle, s'est orienté vers la recherche de souches fongiques tolérantes et efficaces dans la dégradation du fluorène puis vers l'amélioration de la biodisponibilité du fluorène en utilisant des surfactifs et des cyclodextrines. Ce travail de sélection en milieu liquide a été suivi d'applications en suspension de sol. Au cours des essais de dégradation du fluorène (5 mg/l), treize souches parmi les trente et une testées se sont révélées avoir un potentiel de dégradation supérieur ou égal à 60 % (après 48 h de contact). Dix neuf souches, à potentiel inférieur ou égal à 60 %, ont fait l'objet d'essais d'amélioration impliquant des surfactifs et des cyclodextrines. La démarche consiste à sélectionner des couples micromycète - surfactif ou cyclodextrine selon des critères de bonne tolérance et d'absence de consommation par les micromycètes, avant de procéder à des essais de dégradation du fluorène. L'application aux sols a permis d'identifier des micromycètes efficaces à partir d'un sol pollué et de réaliser des essais de dégradation en suspension de sol amendé en fluorène (100 æg/g). L'importance de la microflore native dans la dégradation du fluorène et l'effet positif de la bioaugmentation ont ainsi pu être mis en évidence. L'utilisation de maltosyl cyclodextrine et de Tween 80 a également un impact positif sur la biodépollution.
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Lépine, Guylaine. "Étude des gènes de la famille HAP chez Physarum polycephalum." Master's thesis, Université Laval, 1989. http://hdl.handle.net/20.500.11794/57512.
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Mazur, Stéphane. "Caractéristiques moléculaires du génome du polyomavirus de hamster (HaPV) associées à son pouvoir lymphomatogène." Paris 11, 1995. http://www.theses.fr/1995PA11T001.
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Sadiku, Pranvera. "The regulation of myeloid cell survival : emerging roles for the pro-survival protein HAX1." Thesis, University of Sheffield, 2014. http://etheses.whiterose.ac.uk/8004/.
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Svensson, Elin. "Påverkas utövandet av fysisk aktivitet av psykologiska faktorer? : En kvantitativ undersökning av HAPA-modellen." Thesis, Linnéuniversitetet, Institutionen för psykologi (PSY), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-71801.
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Lukic, Borislava. "Compostage de déchets organiques avec des sols contaminés par des hydrocarbures aromatiques polycycliques (HAP) : impact de l'origine des déchets sur les rendements de biodégradation des HAP." Thesis, Paris Est, 2016. http://www.theses.fr/2016PESC1134/document.
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Les hydrocarbures aromatiques polycycliques (HAP) sont des polluants organiques largement répandus dans l’environnement. Ils sont très fréquemment détectés dans les sols et sont principalement le produit d’une combustion incomplète de la matière organique. Etant toxiques et cancérigènes, de nombreuses études portant sur leur élimination des sols ont été massivement effectuées au cours des dernières années. Parmi tous les traitements disponibles pour traiter des sols contaminés par les HAP, les approches biologiques sont prometteuses car elles ont un impact limité, voire nul sur l’environnement. Cependant, leur efficacité est étroitement dépendante de nombreux facteurs difficiles à contrôler. L’objectif de cette thèse a été d’obtenir une connaissance plus précise concernant la dépollution biologique de sols contaminés par des HAP, en définissant à travers une série d‘expériences, les conditions les plus appropriées pour leur élimination principalement en termes de caractéristiques physiques et chimiques du sol, de la structure des HAP et leurs concentrations, la densité et la composition microbienne, le pH et l’humidité du sol et la disponibilité des nutriments. Les expériences réalisées dans cette thèse, sont basées sur le compostage de déchets organiques avec des sols contaminés par des HAP. L’ajout de matière organique vise à promouvoir la dégradation biologique simultanée des HAP et des déchets organiques frais en conditions contrôlées. Les résultats attendus de cette approche sont la dégradation des polluants en composés moins nocifs, en raison de la stimulation de l’activité des micro-organismes présents dans le sol ainsi que dans les déchets organiques apportés. Dans le but de comprendre l’influence des facteurs précédemment mentionnés, les expériences ont été menées sur un sol synthétique, artificiellement contaminé, ainsi que sur un sol contaminé provenant d’un site industriel identifié comme pollué par des HAP. Quatre types de déchets organiques frais ont été sélectionnés pour être ajoutés au sol artificiellement contaminé par des HAP. Les résultats ont montré que les boues activées étaient l’amendement organique le plus efficace par rapport au fumier de bufflonnes, aux déchets de cuisine et aux déchets organiques à base de légumes. Un taux d’élimination des HAP totaux supérieur à 60% a été atteint avec les boues activées. En outre, cette série d’expériences a prouvé que les conditions mésophiles étaient plus favorables que les conditions thermophiles, mais également que la teneur en azote, l’importance de la fraction soluble et les teneurs en protéines sont très importantes pour l’élimination des HAP. Sur la base de ces résultats, une série d’expériences a été menée sur un sol historiquement contaminé en apportant des quantités différentes de boues activées (ratio massique sol contaminé : boues activées variant de 1:2, 1:1 , 1:0,5 à 1:0). Contrairement aux résultats obtenus avec le sol artificiel, pour un sol naturellement contaminé, les amendements à base de boues activées n’ont pas stimulé l’élimination de HAP. Les meilleurs résultats ont été obtenu pour le sol non amendé par les boues activées (un rendement d’élimination des HAP totaux de 32% a été atteint en l’absence de boues activées, tandis qu'en présence d’un amendement à base de boues activées les meilleurs résultats ne dépassent pas 14% d’élimination des HAP totaux), cela prouve que la stimulation de micro-organismes pouvant dégrader les HAP est un facteur clé pour le succès du processus de dépollution biologique dans des conditions environnementales favorablesPolycyclic Aromatic Hydrocarbons (PAHs) are organic pollutants widely distributed in the environment and very frequently detected in soils as they are mainly produced from an incomplete combustion of organic matter at high temperature. They are toxic and even carcinogenic, therefore their removal from soils has been massively studied in the past years. Among all treatments suitable for treating soils contaminated by PAHs, biological ones are promising and challenging as they have a low or even no impact on the environment as well as their efficiency is tightly dependent on many factors not easy to control. The objective of this thesis has been to get more accurate knowledge on bioremediation of PAHs contaminated soils, by defining, through a series of experiments, the most suitable conditions for their biological remediation mainly in terms of physical and chemical soil characteristics, PAHs type and concentration, microbial density and composition, pH value, moisture content and availability of nutrients. The bioremediation experiments carried out in this thesis are based on landfarming as well as composting soil treatments and aimed at promoting the simultaneous biodegradation of PAHs and fresh organic wastes under controlled conditions. The expected result of this approach has been the conversion of organic pollutants into less harmful compounds, due to the activity of the microorganisms present in soil as well as in the organic waste added to soil. With the aim of deeply understanding the influence of the aforementioned factors on the bioremediation of PAHs contaminated soil, experiments were conducted on a synthetic soil, artificially contaminated, as well as on a real contaminated soil. In details, four different types of fresh organic waste were selected to be added to PAHs artificially contaminated synthetic soil and the results showed that centrifuged activated sewage sludge (SS) was the most effective organic amendment compared to buffalo manure (BM), food and kitchen waste (FKW) and fruit and vegetable waste (FVW). The removal efficiency of total PAHs reached with SS was actually a little higher than 60%. Furthermore, this set of experiments proved that mesophilic conditions were more performing than thermophilic conditions, as well as the content of nitrogen, soluble fraction and proteins played an important role in the PAHs removal. Based on the previous results, the next set of experiments was conducted on a real contaminated soil amended with different amounts of centrifuged activated SS (e.g. contaminated soil to SS mass ratios were 1:2, 1:1, 1:0.5 and 1:0 as wet weight basis). In contrast to results obtained with synthetic soil, in a real contaminated soil, SS amendment resulted in being not beneficial to PAHs removal. The best results were actually achieved where no SS was added (total PAHs removal efficiency of 32%, whereas with SS the best results showed a value of 14%), proving that the adaptation of microorganisms to PAHs is the key factor for the success of the bioremediation process coupled with setting favorable environmental conditions
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Tarantini, Adeline. "Modulation de la génotoxicité des hydrocarbures aromatiques polycycliques (HAP) en mélanges." Phd thesis, Grenoble 1, 2009. http://tel.archives-ouvertes.fr/tel-00456384.
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Les Hydrocarbures Aromatiques Polycycliques (HAP) sont une famille de polluants atmosphériques issus de la combustion incomplète de la matière organique. Le chauffage domestique, la fumée de tabac ainsi que certaines activités industrielles sont les principales sources d'émission environnementale. Certains HAP sont connus ou suspectés d'être cancérigènes pour l'homme, comme le benzo[a]pyrene (BaP), à travers la formation de dommages à l'ADN. La majorité des études se sont focalisées sur l'étude d'un HAP pur alors que l'exposition est toujours en lien avec des mélanges. Ce travail vise à évaluer in vitro sur un modèle d'hépatocytes humains (lignée HepG2) la modulation de la génotoxicité des HAP en mélanges à travers l'utilisation de deux biomarqueurs : le test des comètes mesurant les cassures de l'ADN et le dosage par HPLC/MS/MS des adduits à l'ADN du BPDE, métabolite principal du BaP. Dans un premier temps, les dommages induits par le BaP pur ou en mélange binaire avec d'autres HAP particulaires sont évalués. L'étude est ensuite étendue d'une part à des mélanges atmosphériques réels prélevés dans l'environnement ou en milieu industriel, et d'autre part à la fraction d'HAP particulaires reconstituée correspondante. Dans une dernière partie, l'intérêt de ces tests in vitro pour le suivi de l'exposition des populations à des agents génotoxiques associés à divers types d'environnements a été évaluée. Ce travail met en avant la nécessité d'approfondir les connaissances sur les mécanismes à l'origine des interactions entre les composés ainsi que l'intérêt du recours à la modélisation comme outil d'évaluation des dangers liés à l'exposition des populations aux HAP en mélanges.
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Aviat, Florence. "Étude d'une protéine de leptospires : Hemolysis Associated Protein 1 : Hap 1." Nantes, 2006. http://www.theses.fr/2006NANT2012.
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La leptospirose est une zoonose de représentation mondiale concernant la plupart des Mammifères dont l'homme. Hap1 a été purifiée dans une fraction protéique de 32 kDa extraite de leptospires, bactéries responsables de la leptospirose. La protéine Hap1 s'est révélée être associée à une protection hétérologue contre la leptospirose chez des gerbilles. Le gène hap1 a été exprimé par E. Coli afin de produire la protéine recombinante rHap1. Ce travail confirme et prolonge les travaux antérieurs : Hap1 est secrétée par les seuls leptospires pathogènes lors de leur multiplication. Très immunogène et protectrice dans les conditions naturelles, cette protéine sous sa forme recombinante ne permet pas de reproduire les activités protectrices dans les conditions naturelles. L'objectif de ce travail était donc de purifier la ou les formes naturelles de la protéine Hap1 afin d'en définir les présentations structurales expliquant cette contradictionLeptospirosis is a zoonosis with a worldwide distribution concerning most Mammalians among which humans. Hap1 was purified from an antigenic zone with an apparent molecular mass of 32 kDa extracted from leptospires, bacteria responsible for leptospirosis. Immunization with Hap1 expressed by adenovirus or plasmid induced in gerbils a protection against leptospirosis. Then hap1 gene was expressed by E. Coli to product the recombinant protein rHap. This present work confirms these previous data: Hap1 is secreted during the multiplication of only pathogenic leptospires. Very immunogenic and protective in the natural conditions, this protein in the recombinant form doesn't permit to reproduce protective activity in the natural conditions. So, the purpose of this work was to purify one or many Hap1 naturally forms in order to determine the structural difference to understand this contradiction
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Lai, Tram B. "Enhancements to the Microbial Source Tracking Process through the Utilization of Clustering and k-Nearest Clusters Algorithm." DigitalCommons@CalPoly, 2018. https://digitalcommons.calpoly.edu/theses/1813.
Full textAbstract:
Bacterial contamination in water sources is a serious health risk and the sources of the bacterial strains must be identified to keep people safe. This project is the result of a collaboration effort at Cal Poly to develop a new library-dependent Microbial Source Tracking method for determining sources of fecal contamination in the environment. The library used in this study is called Cal Poly Library of Pyroprints (CPLOP). The process of building CPLOP requires students to collect fecal samples from a multitude of sources in the San Luis Obispo area. A novel method developed by the biologists at Cal Poly called pyroprinting is then applied on the two intergenic regions of the E. coli isolates from these samples to obtain their fingerprints. These fingerprints are stored in the CPLOP database. In our study, we consider any E. coli samples whose fingerprints match above a certain threshold to be in the same group of bacterial strain. However, there has not yet been a final MST method that produces an acceptable level of accuracy. In this thesis, we propose a two-step MST classifier that combines two previous works: pyro-DBSCAN and k-RAP. These algorithms were developed specifically for CPLOP. We call our classifier HAP - Hybrid Algorithm for Pyroprints. The classifier works as follows. Given an unknown isolate, the first step requires performing clustering on the known isolates in the library and comparing the unknown isolate against the resulting clusters. If the isolate falls into a cluster, its classification will be returned as the dominant species of that cluster. Otherwise, we apply the k-Nearest Clusters Algorithm on this isolate to determine its final classification. Ultimately, HAP provides us a set of 16 decision strategies that identify the host species of an unknown sample with high accuracy.
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Happ, Sebastian [Verfasser], and Erwin [Akademischer Betreuer] Strasser. "Messung von thrombozytären Mikropartikeln und der Mikropartikel-Aktivität bei Thrombozytapheresen / Sebastian Happ. Betreuer: Erwin Strasser." Erlangen : Universitätsbibliothek der Universität Erlangen-Nürnberg, 2012. http://d-nb.info/1029374392/34.
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Happ, Sonja Verfasser], Antonello [Akademischer Betreuer] [Monti, and Andrea [Akademischer Betreuer] Benigni. "A scalable simulation method for cyber-physical power systems / Sonja Happ ; Antonello Monti, Andrea Benigni." Aachen : Universitätsbibliothek der RWTH Aachen, 2020. http://d-nb.info/1226160034/34.
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Raoux, Christophe. "Modélisation du mécanisme de contamination par les hydrocarbures aromatiques polycycliques HAP des sédiments marins côtiers de Méditerranée : conséquences sur la biodisponibilité des HAP dans le milieu marin." Bordeaux 1, 1991. http://www.theses.fr/1991BOR10529.
Full textAbstract:
Onze hydrocarbures aromatiques polycycliques (hap) ont ete recherches dans differents environnements marins cotiers de mediterranee definissant un gradient de contamination. L'origine principalement pyrolytique des hap contenus dans les sediments est mise en evidence pour l'ensemble des sites, et des pollutions petrogeniques ponctuelles ont ete identifiees dans les sites peu contamines. L'hypothese de l'introduction des hap dans le milieu marin sous forme essentiellement particulaire permet de modeliser les mecanismes de contamination des sediments a l'aide des processus hydrosedimentaires. Les distributions des hap dans les tissus de moules et les niveaux de l'activite enzymatique benzo(a)pyrene monooxygenase indiquent que le mode de contamination des moules par les hap sous forme dissoute est preponderant. Enfin le potentiel eleve de toxicite des sediments fins des mytylicultures est mis en evidence
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Brassey, Noelle. "From the Golden Gate to the Green Mountains: A Hapa Educational Autobiography and Meta-Critical Reflection." ScholarWorks @ UVM, 2012. http://scholarworks.uvm.edu/graddis/29.
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As a former UC Berkeley undergraduate and a University of Vermont graduate student, this is an educational autobiography of a self-identified Hapa, or mixed-race Asian American, through the lens of race and identity. Exploring what it means to be “white” and “privileged,” and realizing that these concepts--like identity--are fluid, this thesis adopts a dual methodology that includes personal narrative, as well as a meta-critical reflection. This thesis focuses on three memoirs: Bone Black and Wounds of Passion by bell hooks, and Hunger of Memory by Richard Rodriguez, each of which explore themes of reclaiming voice and reconstructing identity with regards to race, class, and culture.