Relevant bibliographies by topics / Halophilic bacterium

Academic literature on the topic 'Halophilic bacterium'

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Published: 6 September 2023

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Journal articles on the topic "Halophilic bacterium"

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Ameen, Ayesha, Hira Mubeen, and Shahid Raza. "Halobacterium sp. NRC-1: A Review." International Journal of Advances in Scientific Research 3, no. 3 (March 31, 2017): 30. http://dx.doi.org/10.7439/ijasr.v3i3.4048.

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Halobacteriumsp. NRC-1 is the halophilic bacterium. This halophile has a great potential to survive on high salinity conditions. The genome of this bacterium has sequenced. The studies have done at metagenomics level, which include the Identification and Molecular Characterization. Identification of this extreme halophile was done by 16S ribotyping and PCR amplification using universal primers FD1 and rP1. The sequences than compared with already sequenced genomes of halophiles. The post genomic tools and microarrays were applied for sequencing. Halobacteriumsp. NRC-1 transcriptional profiling showed that it is highly responsive to the environment and give specific responses at gene expression level. There is a need for extensive research at metaproteomic level. To study the expression and function of proteins, whole protein profiling of this specie need to be studied.
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Sorokin, D. Yu, T. P. Tourova, E. A. Galinski, C. Belloch, and B. J. Tindall. "Extremely halophilic denitrifying bacteria from hypersaline inland lakes, Halovibrio denitrificans sp. nov. and Halospina denitrificans gen. nov., sp. nov., and evidence that the genus name Halovibrio Fendrich 1989 with the type species Halovibrio variabilis should be associated with DSM 3050." International Journal of Systematic and Evolutionary Microbiology 56, no. 2 (February 1, 2006): 379–88. http://dx.doi.org/10.1099/ijs.0.63964-0.

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Anaerobic enrichments with acetate as electron donor and nitrate as electron acceptor at 4 M NaCl from inland, hypersaline lake sediments from Central Asia resulted in the isolation of several extremely halophilic bacteria that comprised two subgroups, most with vibrio-shaped cells and a single strain with rod-shaped cells. Members of both subgroups were extremely halophilic, with growth occurring in 2–5 M NaCl with an optimum at 2–3 M. 16S rRNA gene sequence analysis showed a close affiliation of the new isolates with Pseudomonas halophila DSM 3050 in the Gammaproteobacteria. However, phenotypic comparison of the denitrifying halophiles with the original description of P. halophila demonstrated that they were more similar to another bacterium isolated from the same source at the same time, the extremely halophilic Halovibrio variabilis, which has since been reclassified as Halomonas variabilis (DSM 3051). Direct cross-comparison showed that the characteristics of these two halophilic bacteria do not correspond with the original descriptions associated with these names and DSM numbers. While it is desirable that this problem be solved, in connection with the present investigations, this is a matter that can only be solved by a Request for an Opinion. On the basis of the phenotypic and genetic comparison of these isolates, it is proposed that the new denitrifying vibrio-shaped isolates represent a novel species, Halovibrio denitrificans sp. nov. (type strain HGD 3T=DSM 15503T=UNIQEM U232T) and that the rod-shaped isolate represents a novel genus and species, Halospina denitrificans gen. nov., sp. nov. (type strain HGD 1-3T=DSM 15505T=UNIQEM U233T).
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Goel, Usha, Tiiu Kauri, Donn J. Kushner, and Hans-W. Ackermann. "A moderately halophilic Vibrio from a Spanish saltern and its lytic bacteriophage." Canadian Journal of Microbiology 42, no. 10 (October 1, 1996): 1015–23. http://dx.doi.org/10.1139/m96-130.

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A number of bacteria and their phages were isolated from a saltern near Alicante, Spain. One isolate, Vibrio B1, a moderate halophile that is probably a strain of Vibrio costicola, was host to a lytic phage, UTAK. Studies of the host bacterium included the effects of salt concentrations on the action of a number of inhibitory agents. Phage UTAK has a head, a tail, and a baseplate. It contains 80 kbp of double-stranded DNA with no unusual bases. It was stable for long periods in the absence of high salt concentrations and even in distilled water. Salt concentrations had little effect on adsorption of UTAK to its host but resulted in considerable changes in burst size. It appears that phages of halophilic and salt-tolerant eubacteria, and also of some marine bacteria, have much lower salt requirements for stability than the phages of halophilic archaebacteria. Our results suggest that ionic controls of phage replication in these eubacteria may differ from those of growth.Key words: halophiles, Vibrio sp., bacteriophage, salt responses.
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Echigo, Akinobu, Hiroaki Minegishi, Yasuhiro Shimane, Masahiro Kamekura, and Ron Usami. "Natribacillus halophilus gen. nov., sp. nov., a moderately halophilic and alkalitolerant bacterium isolated from soil." International Journal of Systematic and Evolutionary Microbiology 62, no. 2 (February 1, 2012): 289–94. http://dx.doi.org/10.1099/ijs.0.028506-0.

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A moderately halophilic and alkalitolerant bacterium, designated strain HN30T, was isolated from garden soil in Japan. Cells of strain HN30T were motile, endospore-forming, aerobic, rod-shaped and Gram-positive, and contained A1γ meso-diaminopimelic acid-type murein. Growth occurred in 7–23 % (w/v) NaCl (optimum, 10–15 %, w/v), at pH 6.5–10.0 (optimum, pH 8.0–8.5) and at 20–40 °C (optimum, 30 °C). The isoprenoid quinone was menaquinone-7. The polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16 : 0. The DNA G+C content of strain HN30T was 47 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HN30T was most closely related to Geomicrobium halophilum BH1T (93 % sequence similarity). 16S rRNA gene sequence similarities with other recognized species were less than 89 %. Phylogenetic and phenotypic characteristics indicated that strain HN30T represents a novel species in a new genus, for which the name Natribacillus halophilus gen. nov., sp. nov. is proposed; the type strain is HN30T ( = JCM 15649T = DSM 21771T).
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Liu, Wenyan, Linlin Jiang, Chunjing Guo, and Su Sheng Yang. "Terribacillus aidingensis sp. nov., a moderately halophilic bacterium." International Journal of Systematic and Evolutionary Microbiology 60, no. 12 (December 1, 2010): 2940–45. http://dx.doi.org/10.1099/ijs.0.017228-0.

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Three Gram-positive, moderately halophilic bacteria, designated YI7-61T, IA7 and DB2, were isolated from sediments of Aiding salt lake in the Xinjiang region of China. Cells of the strains were rod-shaped, motile by means of peritrichous flagella and produced ellipsoidal spores. Colonies were pale yellow in colour. The strains grew optimally at 30–37 °C, pH 6–7 and 3–7 % (w/v) NaCl. The diamino acid in the murein was meso-diaminopimelic acid and the major quinone system was MK-7. The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content was 44.6–45.0 mol%. 16S rRNA gene sequence analysis revealed that strains YI7-61T, IA7 and DB2 were closely related to members of the genus Terribacillus and showed 96.8–97.6, 96.4–97.2 and 95.4–95.5 % 16S rRNA gene sequence similarity with Terribacillus halophilus 002-051T, Terribacillus saccharophilus RB589 and Terribacillus goriensis CL-GR16T, respectively. DNA–DNA relatedness among the isolates was 88–92 % and strain YI7-61T shared 24, 18 and 18 % DNA–DNA relatedness with T. halophilus JCM 21760T, T. saccharophilus JCM 21759T and T. goriensis DSM 18252T, respectively. On the basis of phenotypic and phylogenetic distinctiveness, the three isolates should be placed in the genus Terribacillus as representatives of a novel species, for which the name Terribacillus aidingensis sp. nov. is proposed. The type strain is YI7-61T (=CGMCC 1.8913T =NBRC 105790T).
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Kim, Minji, Ki-Eun Lee, In-Tae Cha, and Soo-Je Park. "Draconibacterium halophilum sp. nov., A Halophilic Bacterium Isolated from Marine Sediment." Current Microbiology 78, no. 6 (April 27, 2021): 2440–46. http://dx.doi.org/10.1007/s00284-021-02496-8.

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Oh, Young Joon, Joon Yong Kim, Hyo Kyeong Park, Ja-Young Jang, Seul Ki Lim, Min-Sung Kwon, and Hak-Jong Choi. "Salicibibacter halophilus sp. nov., a moderately halophilic bacterium isolated from kimchi." Journal of Microbiology 57, no. 11 (October 28, 2019): 997–1002. http://dx.doi.org/10.1007/s12275-019-9421-z.

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Caumette, Pierre, Renée Baulaigue, and Robert Matheron. "Thiocapsa halophila sp. nov., a new halophilic phototrophic purple sulfur bacterium." Archives of Microbiology 155, no. 2 (January 1991): 170–76. http://dx.doi.org/10.1007/bf00248613.

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Roeβler, Markus, and Volker Müller. "Chloride, a New Environmental Signal Molecule Involved in Gene Regulation in a Moderately Halophilic Bacterium, Halobacillus halophilus." Journal of Bacteriology 184, no. 22 (November 15, 2002): 6207–15. http://dx.doi.org/10.1128/jb.184.22.6207-6215.2002.

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ABSTRACT The gram-positive, aerobic, moderately halophilic bacterium Halobacillus halophilus is challenged in its environment by frequently changing salt (NaCl) concentrations. Recently, H. halophilus was shown to be the first prokaryote that is dependent on Cl− for growth. In a search for the biological function of Cl− in this prokaryote, we identified different Cl−-dependent processes, which suggests a more general role for Cl− in the metabolism of H. halophilus. To analyze the effect of Cl− in more detail, we concentrated on one model system, the Cl−-dependent production of flagella, and aimed to identify the molecular basis for the Cl− dependence of flagellum production. Here, we report that synthesis of the major subunit of the flagellum, FliC, is dependent on the Cl− concentration of the medium, as determined by Western blot analyses. The gene encoding FliC was cloned and sequenced, and Northern blot as well as reverse transcriptase PCR analyses revealed that expression of fliC is Cl− dependent. FliC is the first protein of known function demonstrated to be synthesized in a Cl−-dependent manner in a prokaryote. Two-dimensional gel electrophoresis of cells grown under different conditions revealed five more Cl−-induced proteins; these were identified by N-terminal sequencing and database searches to be orthologs of proteins involved in stress response in Bacillus subtilis. The data indicate that Cl− is an important environmental signal in this moderate halophile and regulates protein synthesis and gene expression. Furthermore, the data may suggest that Cl− plays a role in the signal transduction involved in salt perception by this bacterium.
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ISHIBASHI, Matsujiro, Sayaka YAMASHITA, and Masao TOKUNAGA. "Characterization of Halophilic Alkaline Phosphatase fromHalomonassp. 593, a Moderately Halophilic Bacterium." Bioscience, Biotechnology, and Biochemistry 69, no. 6 (January 2005): 1213–16. http://dx.doi.org/10.1271/bbb.69.1213.

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Dissertations / Theses on the topic "Halophilic bacterium"

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Choquet, Christian. "Physiological studies of a moderately halophilic bacterium, Vibrio costicola." Thesis, University of Ottawa (Canada), 1990. http://hdl.handle.net/10393/5591.

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In vitro protein synthesis in Vibrio costicola (polyuridilic acid (poly(U)) -directed incorporation of phenylalanine) was studied. As shown previously (Kamekura and Kushner, 1984), Cl$\sp-$ ions inhibited protein synthesis. The inhibitory effects were partly reversed by glutamate and betaine, both of which are concentrated within cells of V. costicola. Washing ribosomes with inhibitory NaCl concentrations did not interfere with their ability to carry out protein synthesis later in optimal (low) salt concentrations. The main site of action of Cl$\sp-$ in this system is on the binding of ribosomes to the messenger RNA. I studied the protein synthesizing machinery of V. costicola using cell-free protein synthesis systems directed by natural mRNAs. One system, the endogenous system, was directed by a mixture of unidentified messengers present in V. costicola, the mRNAs endogenous to these cells. In another system, I have shown that the viral mRNA, that of the coliphage R17, directs the incorporation of ($\sp $C) valine with cellular extracts of V. costicola. The ionic requirements of both systems were similar; both were most active at NH$\sb4\sp+$ (as ammonium glutamate) and Mg$\sp{++}$ concentrations of 250-300 mM and 8 mM, respectively. As was the case with the poly(U) system, Cl$\sp-$ also inhibited protein synthesis in these systems. Added sodium or potassium glutamate inhibited the R17 RNA system but stimulated the endogenous system. It is believed that Cl$\sp-$ is more deleterious to the initiation than to the elongation of protein synthesis, and that glutamate stimulates elongation. Betaine stimulated the activity of both systems, but did not show the same protective effects against Cl$\sp-$ as those observed in the poly(U) system. I have successfully isolated a crude preparation of initiation factors of V. costicola. These factors were released from the ribosomes at a NH$\sb4$Cl concentration much higher than that required for the isolation of the initiation factors of E. coli (1.0 M). Cl$\sp-$ was more inhibitory to the retention of ($\sp $C) fmet-tRNA on nitrocellulose filters, than to the retention of ($\sp3$H) R17 RNA; the retention of these substances was presumably due to the action of IF$\sb2$ and IF$\sb3$, respectively. The enzymes choline dehydrogenase and betaine aldehyde dehydrogenase, which are involved in the synthesis of betaine from choline, were identified in V. costicola. Both enzymes are probably inducibly formed in the presence of choline and seem to be osmoregulated. V. costicola accumulates betaine in response to increasing external NaCl; the intracellular betaine concentration in cells grown in media containing 1.0 and 3.0 M NaCl is 0.25 and 1.2 M, respectively. This accumulation of betaine is also affected by the external concentration of choline. At the lowest choline concentration (0.002%), there is no difference in betaine concentration between cells grown in media containing 0.5, 1.0 and 2.0 M NaCl, while cells grown in the presence of 3.0 M NaCl have only slightly more (0.4 M). These results suggest that betaine may not be the only compatible solute in V. costicola and/or that it may be synthesized through an alternative pathway, and that these cells may also employ other means to maintain turgor pressure. (Abstract shortened by UMI.)
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Aston, John. "Response to osmotic stress by the haloalkaliphilic bacterium Halomonas campisalis." Online access for everyone, 2006. http://www.dissertations.wsu.edu/Thesis/Spring2006/j%5Faston%5F031406.pdf.

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Albaugh, Catherine Elizabeth. "Degradation of monoaromatic compounds by an aerobic halotolerant alkaliphilic bacterium." Online access for everyone, 2005. http://www.dissertations.wsu.edu/Thesis/Summer2005/c%5Falbaugh%5F081905.pdf.

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Thomas, Tatiana. "Étude du potentiel biotechnologique de Halomonas sp. SF2003 : application à la production de polyhydroxyalcanoates (PHA) Complete genome sequence of the halophilic PHA-producing bacterium Halomonas sp. SF2003: insights into its biotechnological potential PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003." Thesis, Lorient, 2019. http://www.theses.fr/2019LORIS542.

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L’amenuisement des ressources pétrochimiques couplé à la pollution engendrée par l’exploitation des plastiques posent de nombreuses questions et conduisent à un besoin urgent de solutions alternatives. Les polyhydroxyalcanoates (PHA) sont des polymères qui ont su se démarquer et naturellement s’imposer comme matériaux de remplacement, de par leurs caractères à la fois biosourcé et biodégradable. Leur synthèse par un grand nombre d’organismes procaryotes et eucaryotes, à partir d’une large gamme de substrats carbonés, rend leur production quasi illimitée et conduit à l’obtention de polymères aux propriétés allant de celles d’un thermoplastique rigide à un élastomère. Malgré cela, les rendements et les coûts de production représentent toujours les principaux verrous à leur développement. Parmi les solutions envisagées, l’exploitation de ressources marines, telles que les bactéries halophiles, suscite un fort intérêt tant les capacités d’adaptation de ces souches sont étendues et avantageuses. Halomonas sp. SF2003 est une souche marine naturellement productrice de PHA possédant une grande capacité d’adaptation face à de nombreux substrats et conditions environnementales. L’étude et l’optimisation de sa production de PHA font l’objet de ce travail. Le séquençage de son génome, couplé à des tests phénotypiques, ont permis l’étude de différents gènes et voies métaboliques confirmant son caractère adaptatif. Dans le même temps, l’influence de différents paramètres sur la production de PHA a été étudiée. Ces travaux, faisant appel à des techniques de biologie moléculaire et des bioprocédés, contribueront au développement du potentiel biotechnologique de la souche Halomonas sp. SF2003
Depletion of oil ressources coupled to pollution caused by over-exploitation of plastics generate a plentiful of issues and lead to an urgent need for alternatives. PolyHydroxyAlkanoates (PHA) are biopolymers which have distinguished and naturally imposed themselves due to their biosourcing and biodegradability features. Their synthesis by a wide variety of eukaryotic and prokaryotic organisms, from various carbon substrates, makes their production almost unlimited and allows obtaining polymers exposing rigid thermoplastic to elastomeric properties. Despite that, the production yield and the cost are still the main locks to their development. Among possibilities studied, exploitation of marine resources, like halophilic bacteria, arouse a strong interest since adaptability of these strains is extensive and attractive. Halomonas sp. SF2003 is a PHA producing marine bacterium which naturally exposes a considerable versatility in front of carbon substrates and environmental conditions. Characterization and optimization of its PHA production are the main subjects of the presented study. Genome sequencing and annotation, in addition to phenotypic tests, allowed characterization of various genes and metabolic pathways attesting of the adaptative strain character. In the same time, impact of various parameters on Halomonas sp. SF2003 PHA production has been investigated. This work, employing molecular biology and bioprocess tools, will contribute to future development of biotechnological potential of Halomonas sp. SF2003
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Kadam, Priya. "Physiology of halophilic, methylotrophic methanogens /." Full text open access at:, 1996. http://content.ohsu.edu/u?/etd,652.

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Richards, Abigail Marie. "Identification and structural characterization of siderophores produced by halophilic and alkaliphilic bacteria." Online access for everyone, 2007. http://www.dissertations.wsu.edu/Dissertations/Summer2007/A_Richards_072707.pdf.

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Cruzeiro, Sofia Maria de Albuquerque. "Isolation and characterization of halophilic bacteria from salt pans of Ria de Aveiro." Master's thesis, Universidade de Aveiro, 2018. http://hdl.handle.net/10773/22004.

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Mestrado em Microbiologia
Halophilic microorganisms and the metabolites involved in their adaptive strategies offer a wide variety of potential biotechnological applications and like other extreme environments, hypersaline habitats represent reservoirs of new bioactive compounds. The main objective of this work was to assess the biotechnological potential of halophilic bacteria from traditional salt pans of Ria de Aveiro in terms of biosurfactant production, plant growth promoting traits, quorum-quenching effect and extracellular enzymatic activity. Water from an active salt pan of Ria de Aveiro was collected in early autumn when the concentration of salt was at the saturation level. Using a culture-dependent approach, 14 bacterial strains were isolated and identified by sequencing of 16S rRNA gene fragments as species of Bacillus, Halobacillus, Idiomarina and Marinobacter. In order to assess the biotechnological potential of these halophiles, they were tested for biosurfactant production, plant growth promoting traits (motility, biocontrol effect and siderophore production), quorum-quenching effect and, activity of extracellular enzymes (phosphatase and esterase). None of the isolates demonstrated significant tensioactive or emulsification effects that could be interpreted as indicative of biosurfactant production. However, all isolates were motile even in presence of 10% NaCl and produced siderophores. All Idiomarina and Marinobacter strains caused growth inhibition of the phytopathogenic fungus Alternaria. Quorum sensing inhibition was also detected in isolates of the 4 genera although with differences between species and, in some cases, depending on the salinity of the culture medium. Esterase and phosphatase activity was detected in all isolates and maximum hydrolysis rates of the model substrates were the highest in Halobacillus species. Although biosurfactant production could not be demonstrated, the halophilic isolates displayed an interesting set of features in terms of plant-growth promoting traits with potential application on the emerging field of saline agriculture.
Os microrganismos halofílicos e os metabolitos envolvidos nas suas estratégias adaptativas apresentam uma ampla gama de potenciais aplicações biotecnológicas e, tal como outros ambientes extremos, os habitats hipersalinos são vistos como um reservatório de novos compostos bioativos. O objetivo deste trabalho foi avaliar o potencial biotecnológico de bactérias halofílicas de salinas da Ria de Aveiro em termos de produção de biossurfactantes, características de promoção do crescimento de plantas, efeito de quorum-quenching e atividade enzimática extracelular. No início do outono de 2016, quando a concentração de sal atinge os valores máximos colheu-se água do tanque de cristalização de uma salina ativa da Ria de Aveiro (Marinha de Santiago da Fonte). Utilizando uma abordagem dependente de cultivo, foram isoladas 14 estirpes bacterianas que foram posteriormente identificadas por sequenciação de fragmentos de genes de rRNA 16S como espécies de Bacillus, Halobacillus, Idiomarina e Marinobacter. Para avaliar o potencial biotecnológico destas halófilas, testou-se a produção de biossurfactantes, características de promoção do crescimento de plantas (mobilidade, efeito de biocontrolo e produção de sideróforos), efeito de quorum-quenching e atividade enzimática extracelular (fosfatase e esterase). Nenhum dos isolados demonstrou efeitos tensioativos ou de emulsificação significativos que pudessem ser interpretados como indicativos da produção de biossurfactantes. No entanto, todos os isolados exibiram mobilidade, mesmo na presença de 10% de NaCl e produziram sideróforos. Todas as estirpes de Idiomarina e Marinobacter causaram inibição do crescimento do fungo fitopatogénico Alternaria. A inibição de quorum sensing também foi detetada em isolados dos 4 géneros, embora com diferenças entre espécies e, em alguns casos, dependendo da salinidade do meio de cultura. A atividade de esterases e da fosfatases foi detetada em todos os isolados e as mais altas taxas de hidrólise dos substratos modelo foram observadas nas espécies de Halobacillus. Embora a produção de biossurfactantes não tenha sido demonstrada, os isolados halofílicos exibem um conjunto de características interessantes em termos de promoção do crescimento de plantas, com potencial aplicação no campo emergente da agricultura salina.
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Tregoning, George Seibert. "Isolation and Characterization of Halophilic Heterotrophic Bacteria from Lake Vanda, McMurdo Dry Valleys, Antarctica." OpenSIUC, 2010. https://opensiuc.lib.siu.edu/theses/395.

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Lake Vanda is a meromictic, permanently ice-covered lake in the McMurdo Dry Valleys. The McMurdo Dry Valleys are a polar desert in Southern Victorialand Antarctica. This area experiences very little rainfall and very cold average temperatures, around –20°C. Lake Vanda has an unusual limnological profile, with a sharp physical and chemical gradient at about 60 m where the water transitions from cold, oxic, and fresh, to warm, hypersaline, and sulfidic; CaCl2 rather than NaCl is the dominant salt. Aerobic enrichment techniques were used to isolate what turned out to be several strains of a species of Chromohalobacter, a genus of the Gammaproteobacteria, from Lake Vanda deep waters, while targeted enrichments for anaerobic and spore-forming bacteria were negative. The isolates were characterized for their temperature and pH optima, carbon and nitrogen nutrition, and salt tolerance and requirements. The results showed the organisms to be obligately aerobic with a broad pH range (optima pH 7). The isolates used some sugars and organic acids but not alcohols or fatty acids for energy and cell carbon, and showed a moderate requirement for NaCl but no requirement for CaCl2, even though CaCl2 is the predominant salt in their environment.
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Paul, Varun. "Electricity generation and ethanol production using iron-reducing, haloalkaliphilic bacteria." Diss., Rolla, Mo. : Missouri University of Science and Technology, 2009. http://scholarsmine.mst.edu/thesis/pdf/Paul_09007dcc8069dfe5.pdf.

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Thesis (M.S.)--Missouri University of Science and Technology, 2009.
Vita. The entire thesis text is included in file. Title from title screen of thesis/dissertation PDF file (viewed August 10, 2009) Includes bibliographical references (p. 58-64).
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Abosmaha, Mohammed Abdolkader. "Molecular identification and physiological characterization of halophilic and alkaliphilic bacteria belonging to the genus Halomonas." Thesis, University of Sheffield, 2013. http://etheses.whiterose.ac.uk/4647/.

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Alkaline saline lakes are unusual extreme environments formed in closed drainage basins. Qabar - oun and Um - Alma lakes are alkaline saline lakes in the Libyan Sahara. There were only a few reports (Ajali et al., 1984) on their microbial diversity before the current work was undertaken. Five Gram-negative bacterial strains, belonging to the family of Halomonadaceae, were isolated from the lakes by subjecting the isolates to high salinity medium, and identified using 16S rRNA gene sequencing as Halomonas pacifica, Halomonas sp, Halomonas salifodinae, Halomonas elongata and Halomonas campisalis. Two of the Halomonas species isolated (H. pacifica and H. campisalis) were chosen for further study on the basis of novelty (H. pacifica) and on dual stress tolerance (high pH and high salinity) shown by H. campisalis. Both species showed optimum growth at 0.5 M NaCl, but H. campisalis alone was able to grow in the absence of NaCl. H. pacifica grew better than H. campisalis at high salinities in excess of 1 M NaCl and was clearly a moderately halophile. H. pacifica showed optimum growth at pH 7 to 8, but in contrast H. campisalis could grow well at pH values up to 10. 13C - NMR spectroscopy was used to determine and identify the compatible solutes accumulated by H. pacifica and H. campisalis grown in rich and minimal media at different concentrations of NaCl. H. pacifica and H. campisalis accumulated betaine in rich (LB) medium with ectoine only appearing at the highest salinity tested (2.5 M NaCl). In contrast, in M9 minimal medium, no betaine was detected and ectoine and hydroxyectoine were accumulated at high salinities. H. campisalis was able to grow well with urea or nitrate as the sole source of nitrogen and was shown to be capable of efficiently removing nitrate from the medium under aerobic assimilatory conditions, where it is incorporated into biomass.
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Books on the topic "Halophilic bacterium"

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H, Vreeland Russell, and Hochstein Lawrence I, eds. The Biology of halophilic bacteria. Boca Raton, Fla: CRC Press, 1992.

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1952-, Oren Aharon, Ma Yanhe, and SpringerLink (Online service), eds. Halophiles and Hypersaline Environments: Current Research and Future Trends. Berlin, Heidelberg: Springer-Verlag Berlin Heidelberg, 2011.

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United States. National Aeronautics and Space Administration., ed. The evolution of energy-transducing systems: Studies with an extremely halophilic archaebacterium : final report, February 1989 - August 1992, NASA cooperative agreement number, NCC 2-578. Mountain View, CA: SETI Institute, 1992.

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United States. National Aeronautics and Space Administration., ed. The evolution of energy-transducing systems: Studies with an extremely halophilic archaebacterium : final report, February 1989 - August 1997, NASA cooperative agreement number: NCC 2-578. [Washington, DC: National Aeronautics and Space Administration, 1997.

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United States. National Aeronautics and Space Administration., ed. The evolution of energy-transducing systems: Studies with an extremely halophilic archaebacterium : final report, February 1989 - August 1992, NASA cooperative agreement number, NCC 2-578. Mountain View, CA: SETI Institute, 1992.

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6

United States. National Aeronautics and Space Administration., ed. The evolution of energy-transducing systems: Studies with an extremely halophilic archaebacterium : final report, February 1989 - August 1997, NASA cooperative agreement number: NCC 2-578. [Washington, DC: National Aeronautics and Space Administration, 1997.

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United States. National Aeronautics and Space Administration., ed. The evolution of energy-transducing systems: Studies with an extremely halophilic archaebacterium : final report, February 1989 - August 1997, NASA cooperative agreement number: NCC 2-578. [Washington, DC: National Aeronautics and Space Administration, 1997.

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8

United States. National Aeronautics and Space Administration., ed. The evolution of energy-transducing systems: Studies with an extremely halophilic archaebacterium : semiannual progress report, February 1991-July 1991, NASA cooperative agreement number, NCC 2-578. Mountain View, CA: SETI Institute, 1991.

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9

United States. National Aeronautics and Space Administration., ed. The evolution of energy-transducing systems: Studies with an extremely halophilic archaebacterium : semiannual progress report, February 1991-July 1991, NASA cooperative agreement number, NCC 2-578. Mountain View, CA: SETI Institute, 1991.

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Francisco, Rodriguez-Valera, ed. Halophilic bacteria. Boca Raton, Fla: CRC Press, 1988.

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Book chapters on the topic "Halophilic bacterium"

1

Oren, Aharon, Francisco Rodríguez-Valera, Josefa Antón, Susana Benlloch, Ramon Rosselló-Mora, Rudolf Amann, Julie Coleman, and Nicholas J. Russell. "Red, Extremely Halophilic, but not Archaeal: The Physiology and Ecology of Salinibacter ruber, a Bacterium Isolated from Saltern Crystallizer Ponds." In Halophilic Microorganisms, 63–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-662-07656-9_4.

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Köcher, Saskia, and Volker Müller. "The Nature and Function of Carotenoids in the Moderately Halophilic Bacterium Halobacillus halophilus." In Halophiles and Hypersaline Environments, 303–17. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-662-45796-2_16.

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Müller, Volker, and Saskia Köcher. "Adapting to Changing Salinities: Biochemistry, Genetics, and Regulation in the Moderately Halophilic Bacterium Halobacillus halophilus." In Extremophiles Handbook, 383–400. Tokyo: Springer Japan, 2011. http://dx.doi.org/10.1007/978-4-431-53898-1_18.

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Xu, Gaochao, Yuangao Deng, Donghui Song, and Liying Sui. "Isolation and Characterization of a New Moderately Halophilic Bacterium Strain SM. 200-5 from Solar Saltern Ponds." In Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), 1023–32. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37922-2_109.

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Quesada, Emilia, Victoria Béjar, M. Rita Ferrer, Concepción Calvo, Inmaculada Llamas, Fernando Martínez-Checa, Soledad Arias, et al. "Moderately Halophilic, Exopolysaccharide-Producing Bacteria." In Halophilic Microorganisms, 297–314. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-662-07656-9_22.

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Kunte, Hans Jörg. "Osmoregulated Solute Transport in Halophilic Bacteria." In Halophilic Microorganisms, 155–64. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-662-07656-9_10.

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Zavarzin, George A., Tatjana N. Zhilina, and Margarete A. Pusheva. "Halophilic Acetogenic Bacteria." In Acetogenesis, 432–44. Boston, MA: Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-1777-1_16.

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Pfeifer, Felicitas. "Gas Vesicle Genes in Halophilic Archaea and Bacteria." In Halophilic Microorganisms, 229–41. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-662-07656-9_16.

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Mellado, E., C. Sánchez-Porro, S. Martín, and A. Ventosa. "Extracellular Hydrolytic Enzymes Produced by Moderately Halophilic Bacteria." In Halophilic Microorganisms, 285–95. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-662-07656-9_21.

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Peyton, Brent M., Melanie R. Mormile, Victor Alva, Celso Oie, Francisco Roberto, William A. Apel, and Aharon Oren. "Biotransformation of Toxic Organic and Inorganic Contaminants by Halophilic Bacteria." In Halophilic Microorganisms, 315–31. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-662-07656-9_23.

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Conference papers on the topic "Halophilic bacterium"

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Fedonenko, Yu P., I. M. Ibrahim, E. N. Sigida, V. I. Safronova, M. S. Kokoulin, A. Yu Muratova, and S. A. Konnova. "Bioremediation potential of a halophilic bacterium Chromohalobacter salexigens EG1QL3: exopolysaccharide production, crude oil degradation, and heavy metal tolerance." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.070.

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Based on biochemical and phylogenetic analyses, isolated from a salt sample from Lake Qarun (Egypt) a halophilic strain EG1QL3 was identified as Chromohalobacter salexigens. The abilities of EG1QL3 to produce an extracellular polysaccharide, degrade oil, and resist to heavy metals were revealed.
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DAOUD, Lobna, Adel HADJ BRAHIM, Houda HMANI, Asmahen AKREMI, Mouna JLIDI, Manel BEN ALI, Samir BEJAR, Naser ALIYE FETO, and Mamdouh BEN ALI. "Salicola sp. strain SBJ9: a novel extremely halophilic bacterium with an interesting protease activity." In MOL2NET 2018, International Conference on Multidisciplinary Sciences, 4th edition. Basel, Switzerland: MDPI, 2019. http://dx.doi.org/10.3390/mol2net-04-06115.

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Aleev, Vladislav S., and Elena G. Plotnikova. "GENETIC AND ECOLOGICAL-PHYSIOLOGICAL CHARACTERISTICS OF BACTERIA OF THE GENUS SALINISPHAERA (CLASS GAMMAPROTEOBACTERIA) ISOLATED FROM THE VERKHNEKAMSKY SALT MINER." In Фундаментальные и прикладные исследования в биологии и экологии. Пермский государственный национальный исследовательский университет, 2021. http://dx.doi.org/10.17072/fpibe-2021-4-7.

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5 strains of halophilic bacteria were isolated from clay deposits of brine-diverting workings and brine tanks of the mine of the Verkhnekamskoye salt deposit (Solikamsk, Perm Territory). Because of phylogenetic analysis based on a comparison of the 16S rRNA gene sequences, it was found that the isolated cultures are members of the Salinisphaeraceae family. Three halophilic strains SHV2, RV14, and SWV1 had a similarity with the closest type strain of the Salinisphaera hydrothermalis species at the level of 95.94-96.62% (16S rRNA gene), which indicates that these strains belong to a new taxon. All isolated bacteria are halophiles: they grow at high salinity (up to 270-300 g / l NaCl).
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Drochioiu, Gabi. "THE ROLE OF BACTERIORHODOPSIN IN LIGHT HARVESTING AND ATP PRODUCTION BY HALOBACTERIUM SALINARUM CELLS." In 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022/6.1/s25.17.

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Halobacterium salinarum is an extremely halophilic marine Gram-negative obligate aerobic archaeon. Despite its name, this is not a bacterium, but rather a member of the domain Archaea, which lives in hypersaline lakes. Bacteriorhodopsin (BRh) is the red retinal-containing protein found in the cell membranes of H. salinarum and is considered a light-activated proton pump that transports protons across the plasma membrane. Bacteriorhodopsin photointermediates have been defined in kinetic and spectroscopic terms as BR568, K590, L550, M412, N560, and O640. We have previously shown, using the Forster cycle for BRh that its acidity increases greatly on illumination. Therefore, protons released upon illumination of the L550 intermediate with 412 nm light may not play an essential role in ATP production. Instead, the light-induced excitation energy, which represents the energy difference between the L550 and M412 states, can be used to extract an ATP molecule attached to ATP synthase. Thus, we have shown that this amount of energy corresponds to a near-infrared vibration, which is sufficient for ATP production and provides the most feasible molecular mechanism for this phenomenon. Here, we provide new evidence that protons are released due to BRh excitation, unrelated to ATP synthesis, being only a secondary phenomenon. In addition, once released from H. salinarum cells, protons should return back into the cells via ATP-synthase molecules to produce ATP. This is not possible at pH > 7.0, such as pH 9.5. However, the stability of M intermediates and ATP formation appear to be increased at higher pH values. Indeed, a spectral shift of 138 nm may be associated with an energy amount of about 17 kcal mol-1, which is enough energy to release a mole of ATP from ATP-synthase. In general, light excitation of fluorescent molecules is a phenomenon that induces a strong increase in their acidity. Recent data suggest that the chemiosmotic hypothesis put forward by Peter Mitchell to explain ATP formation in living cells is not correct, at least in terms of explaining light-induced ATP production in H. salinarum cells.
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Maki, Teruya, Shinzi Susuki, Fumihisa Kobayashi, Makiko Kakikawa, Maromu Yamada, Tomomi Higashi, Chunsang Hong, et al. "Composition of halophilic bacteria survived in bioaerosol." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0040.

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Samsonova, E. A., I. M. Ibrahim, Yu P. Fedonenko, and S. A. Konnova. "In vitro evaluation of some halophilic bacterial isolates as biofertilizers." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.215.

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Salam, A. N., F. N. Yuliati, N. Nahariah, R. Malaka, W. Hatta, and K. I. Prahesti. "Halophilic bacteria number and morphology in commercial salted eggs in Makassar." In PROCEEDINGS OF THE 4TH INTERNATIONAL CONFERENCE OF ANIMAL SCIENCE AND TECHNOLOGY (ICAST 2021). AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0144266.

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Khambhaty and Mody. "Enzymatic degradation of carrageenan by halophilic bacteria from South-West coast in India." In Oceans 2003. Celebrating the Past ... Teaming Toward the Future. IEEE, 2003. http://dx.doi.org/10.1109/oceans.2003.178592.

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Klueglein, Nicole, Felix Kögler, Irini J. Adaktylou, Marina L. Wuestner, Eva Mahler, Justus Scholz, Andrea Herold, and Hakan Alkan. "Understanding Selective Plugging and Biofilm Formation of a Halophilic Bacterial Community for MEOR Application." In SPE Improved Oil Recovery Conference. Society of Petroleum Engineers, 2016. http://dx.doi.org/10.2118/179620-ms.

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Li, Xue-Yao, Guang-Hui Ding, Te Wang, and Ling-Hua Zhang. "Resistance to Assistance Characterization to Petroleum Degradation of Moderately Halophilic Bacteria Halomonas sp. B01." In International Conference on New Energy and Sustainable Development (NESD 2016). WORLD SCIENTIFIC, 2016. http://dx.doi.org/10.1142/9789813142589_0071.

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Reports on the topic "Halophilic bacterium"

1

Gillow, J. B., A. J. Francis, C. J. Dodge, R. Harris, T. J. Beveridge, P. V. Brady, and H. W. Papenguth. Actinide biocolloid formation in brine by halophilic bacteria. Office of Scientific and Technical Information (OSTI), December 1998. http://dx.doi.org/10.2172/307836.

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