Relevant bibliographies by topics / Hairy root cultivation

Academic literature on the topic 'Hairy root cultivation'

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Published: 6 September 2023

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Journal articles on the topic "Hairy root cultivation"

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Phan, Hai Trung, Phuong Ngo Diem Quach, and Nhut Nhu Nguyen. "Hairy induction from Impatiens balsamina L. using fourteen Agrobacterium rhizogenes strains." Science and Technology Development Journal 19, no. 2 (June 30, 2016): 38–47. http://dx.doi.org/10.32508/stdj.v19i2.801.

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Impatiens balsamina L. is a plant commonly grown in Vietnam. It has long been used as traditional medicine. All of the secondary metabolites produced by Impatiens balsamina L. root possessed biological activities. The main advantage of using hairy root cultures is their ability of growing fast in defined basal media without supplementation of phytohormones. The aim of this research is to invest some factors that could affect on the hairy root production such as A. rhizogenes strain, type of tissue, OD concentration, immersion time and co-cultivation time. The results showed that 3 strain A. rhizogenes C02, C18 and C26 isolated from nature in Vietnam could induce the best hairy root formation at the 0.5–1.0 OD concentration bacteria with 5’ of immersion time and 72 hours of co-cultivating. Among them, C02 is the strain that could offer the best result in B5 medium
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Bányai, P., I. Bálványos, L. Kursinszki, and E. Szöke. "CULTIVATION OF LOBELIA INFLATA L. HAIRY ROOT CULTURE IN BIOREACTOR." Acta Horticulturae, no. 597 (January 2003): 253–56. http://dx.doi.org/10.17660/actahortic.2003.597.36.

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Ambros, Elena Valerievna, Ol'ga Viktorovna Kotsupiy, Tat'yana Abdulkhailovna Kukushkina, Tat'yana Vital'yevna Zheleznichenko, and Tat'yana Ivanovna Novikova. "SYNTHESIS OF BIOLOGICALLY ACTIVE COMPOUNDS IN HAIRY ROOTS OF ASTRAGALUS PENDULIFLORUS LAM." chemistry of plant raw material, no. 2 (June 10, 2020): 209–21. http://dx.doi.org/10.14258/jcprm.2020026284.

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Agrobacterium rhizogenes – mediated genetic transformation of medicinal plant A. penduliflorus Lam. using A4-RT, R-1601, 15834 SWISS strains was performed. The competences for transformation of three types of explants: hypocotyls, cotyledons, and primary shoots were tested. The virulent strain (15834 SWISS) and types of explants for transformation (primary shoots and cotyledons) with high growth index (I) were determined. The frequency of transformation of cotyledons by strain 15834 SWISS after 4 weeks of cultivation was 15.4% (I = 59.6), hypocotyls – 9.1% (I = 7.3) and primary shoots – 37.5% (I = 21.0). After 8 weeks of cultivation I increased 4.5 times for primary shoots (I = 94.5 ± 0.20) and cotyledons (I = 265.8 ± 0.35), for cultures from hypocotyls – 5.97 times (I = 43.6 ± 0.30). The roots’ transgenic status and the absence of agrobacterium contamination were confirmed by PCR analysis using rolB-, virC-specific primers. The lines of hairy roots characterized by active increases of biomass with high content of biologically active metabolites were selected, moreover, the content of metabolites in hairy root cultures exceeded their content in the roots of introduced plants. The maximum accumulation of compounds was found in hairy roots obtained from primary shoots (pectins – up 7.8%, protopectins – up 15.3%) and cotyledons (tannins – up 16.1%, triterpenic saponins – up 30.5%) after 8 weeks of cultivation. High perfor-mance liquid chromatography (HPLC) analysis demonstrated that hydrolysates of extracts of hairy roots from primary shoots contained 2 flavonol aglycones – quercetin and isorhamnetin whereas ethanol extracts were characterized by presence of quercetin and 4 flavonoid components. In hairy roots from cotyledons the maximum of phenolic compounds (PСs) content did not differ significantly at 8 and 12 weeks of cultivation (1.38 ± 0.01 and 1.49 ± 0.06% of dry weight, respectively). The content of PCs in hairy roots from primary shoots increased two-fold from 4 to 12 weeks of cultivation (up 1.24 ± 0.18%). To the best of our knowledge, this is the first efficient protocol reported for the establishment of hairy root cultures in A. penduliflorus using A. rhizogenes.
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Pavlov, Atanas, Petia Kovatcheva, Vasil Georgiev, Irina Koleva, and Mladenka Ilieva. "Biosynthesis and Radical Scavenging Activity of Betalains during the Cultivation of Red Beet (Beta vulgaris) Hairy Root Cultures." Zeitschrift für Naturforschung C 57, no. 7-8 (August 1, 2002): 640–44. http://dx.doi.org/10.1515/znc-2002-7-816.

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Betalains biosynthesis and antiradical scavenging activity were investigated during cultivation of four hairy root cultures of Beta vulgaris, obtained from different cultivars (Bordo, Egyptian, Detroit 2 and Detroit Dark Red). The best producer of betalains was a hairy root culture from Beta vulgaris cv. Detroit Dark Red (13.27 mg/g dry weight total pigment production). The ethanol extract, derived from roots of the same culture grown for 15 days under submerged conditions, showed a high antiradical activity (83% of inhibition of the stable DPPH·).
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Kabirnataj, Sara, Ghorbanali Nematzadeh, Jafar Zolala, and Ahmad Farhad Talebi. "High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb." Acta agriculturae Slovenica 107, no. 2 (October 26, 2016): 321. http://dx.doi.org/10.14720/aas.2016.107.2.06.

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<p>Plant roots can be manipulated by <em>Agrobacterium rhizogenes</em> to stimulate the production of heterologous proteins for pharmaceutical applications as green cell-factories. During the present study, four bacterial strains (A4, ATCC15834, ATCC11325 and A13) in combination with three co-cultivation media (MS, B5, LS) were examined to establish an efficient and reliable transformation system for chicory (<em>Cichorium intybus</em> L.) using <em>A. rhizogenes</em>. The maximum chicory hairy roots induction was achieved using A13 strain. The observation confirmed that MS medium was more effective on hairy root growth. Dried biomass accumulation of hairy roots infected by A13 strain was 1.10 g l<sup>-1</sup> in MS medium which was significantly higher than those grown in LS and B5 medium (0.88 and 0.72 g l<sup>-1</sup>, respectively). Beta-glucuronidase (GUS) gene was introduced by A13 strain carrying the pCAMBIA1304 binary vector. The results showed that the highest frequency of transformation (63.15 %) was achieved using A13 strain and MS cultivation medium. Detection of GUS and <em>hpt</em>II genes by PCR and GUS histochemical localization confirmed the integrative transformation in hairy roots. In conclusion, the whole process was successfully optimized as a pre-step to manipulate the chicory hairy root cells to improve the unique potential of secondary metabolite production.</p>
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Matvieieva, N. A., O. A. Havryliuk, and L. S. Yastremska. "Effect of short-term cold stress on flavonoid accumulation in Artemisia vulgaris and Artemisia annua “hairy” root cultures." Faktori eksperimental'noi evolucii organizmiv 23 (September 9, 2018): 302–7. http://dx.doi.org/10.7124/feeo.v23.1032.

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Aim. The aim of the work was to investigate the effect of short-term cold stress (+10°С) on the growth, flavonoid synthesis and antioxidant activity in Artemisia vulgaris L. and A. annua L. “hairy“ root cultures. Methods. Transgenic roots were cultivated during the first 1, 2 and 5 days at +10°C on Murashige and Skoog basal medium with twice reduced macrosalt content. The total flavonoids content in Rutin equivalent was determined using alcohol extract reaction with alumunium chloride. Antioxidant activity was studied using the DPPH method. Results. Short-term cold stress resulted in a reduction of mass increment by 12–76 %. The total flavonoid content in «hairy» roots ranged from 32.0±3.13 to 187.0±21.04 mg RE/g dry weight. Decrease of temperature has led to decrease of the flavonoids content in No. 1, No. 2 A. vulgaris root lines and No. 5 A. annua line by 18–33 %. The reaction of No. 3 A. vulgaris and No. 4 A. annua root lines was expressed in stimulation flavonoid synthesis by 62 % and 56.5 %. Cultivation of «hairy» roots under short-term cold stress has led to decrease of the antioxidant activity in all roots lines by 4–40 %. Conclusions. Сold stress had negative effect the “hairy“ roots growth, stimulated flavonoids accumulation only in two “hairy” root lines and reduced the level of antioxidant activity. Keywords: «hairy» roots culture, Artemisia spp., cold stress, flavonoids, antioxidant activity.
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Эрст (Erst), Анна (Anna) Алексеевна (Аlekseevna), Лариса (Larisa) Николаевна (Nikolayevna) Зибарева (Zibareva), Елена (Elena) Сергеевна (Sergeyevna) Филоненко (Filonenko), and Татьяна (Tat'yana) Витальевна (Vital'yevna) Железниченко (Zheleznichenko). "INFLUENCE METHYL JASMONATE ON PRODUCTION OF ECDYSTEROIDS FROM HAIRY ROOTS OF SILENE LINICOLA C.C.GMELIN." chemistry of plant raw material, no. 4 (December 11, 2018): 159–67. http://dx.doi.org/10.14258/jcprm.2018043807.

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Hairy root cultures are being considered as promising system for producing valuable second metabolites. These genetically transformed root cultures are characterized by high growth rate, genetic stability and growth in hormone free media. Using A. rhizogenes-mediated transformation method (strain А-4), we have obtained hairy root cultures of the ecdysteroid-containing species of Silene linicola. HPLC analysis of the sample studied revealed that 20-hydroxyecdysone (20E), turkesterone and polypodin B were synthesized under the specified experimental conditions. Application of methyl jasmonate at a concentration of 100 μM resulted in stimulation of 20E biosynthesis (up 74%) after 3 days of cultivation and turkesterone up to 35% at 6 days. It was noted that total ecdysteroid content in sample tested varied: turkesterone from 25 to 60%, and 20E from 8 to 30%. At the same time the level of 20E biosynthesis decreased from 0.023 to 0.014% in the samples without methyl jasmonate treatment. Hairy root lines of S. linicola with different responses to the presence of elicitors in the culture medium can be used to study the pathways of ecdysteroid biosynthesis.
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Khlebova, L. P., E. S. Brovko, O. V. Bychkova, and N. V. Pavlova. "Optimization of conditions for the induction of Tagetes patula L. hairy roots." Ukrainian Journal of Ecology 9, no. 3 (October 25, 2019): 415–20. http://dx.doi.org/10.15421/2019_119.

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The hairy root cultures are promising sources of secondary metabolites of plants, including rare and endangered species. They possess genetic and biochemical stability, unlimited growth rate in free-hormone medium, short doubling times, high biosynthetic activity and ecological purity of plant raw materials. The hairy root cultures of Tagetes patula L. can be used to produce biologically active substances with biocidal activity. The study aimed to determine the virulent strain of Agrobacterium rhizogenes and the most effective period of co-cultivation of T. patula leaf explants with an agrobacterium to induce actively growing hairy root cultures. We used 3 strains (A-4b, 8196RT and 15834). The time of infection ranged from 3 to 33 hours in increments of 3 hours. We found that 24 h is the best time of infection to induce hairy roots with the highest transformation efficiency (92%). The wild strain A. rhizogenes 15834 turned out to be the most virulent when infected leaf explants of spreading marigold. This strain provided the maximum transformation effect, reaching 85.4%. We have identified 5 actively growing clones of hairy roots with intensive branching, the growth indices of which were 64-75. In the future, they will be transferred to a liquid medium for biomass accumulation and scaling.
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Freitas, Isaac Alves da Silva, Francisco Bezerra Neto, Jailma Suerda Silva de Lima, Jéssica Paloma Pinheiro da Silva, Rayanna Campos Ferreira, and Natan Medeiros Guerra. "Agronomic characteristics and optimized sweet potato root production in monoculture under green manuring." Bioscience Journal 39 (June 9, 2023): e39081. http://dx.doi.org/10.14393/bj-v39n0a2023-66960.

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One of the challenges of the scientific research on sweet potatoes in semi-arid environments is to increase biomass amounts of spontaneous species from the Caatinga biome, such as hairy woodrose (Merremia aegyptia L.) and roostertree (Calotropis procera Ait.), for use as green fertilizers during cultivation. Therefore, this study aimed to agronomically and economically optimize the agronomic characteristics of sweet potato root production in a monoculture, fertilized with equal amounts of biomass mixture of these spontaneous species, over two years of cultivation. The experimental design was complete randomized blocks with five treatments and five replications. The treatments consisted of equal amounts of hairy woodrose and roostertree biomass at 16, 29, 42, 55, and 68 t ha-1 on a dry basis. An additional sweet potato treatment was planted in each experiment, one without fertilizers (control) and another with mineral fertilizer, to compare with the treatment of maximum physical or economic efficiency. Sweet potato fertilization obtained the maximum optimized productive efficiency by incorporating 46.97 t ha-1 of dry biomass of M. aegyptia and C. procera into the soil. The maximum optimized agroeconomic efficiency (based on net income) of sweet potato cultivation occurred by adding 41.55 t ha-1 of dry biomass of M. aegyptia and C. procera to the soil. Using biomass from the green fertilizers M. aegyptia and C. procera is a viable technology for producers who practice sweet potato monocropping in semi-arid environments.
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Urbańska, Natalia, Joanna Giebułtowicz, Olga Olszowska, and Wojciech J. Szypuła. "The growth and saponin production of Platycodon grandiflorum (Jacq.) A. DC. (Chinese bellflower) hairy roots cultures maintained in shake flasks and mist bioreactor." Acta Societatis Botanicorum Poloniae 83, no. 3 (2014): 229–37. http://dx.doi.org/10.5586/asbp.2014.017.

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The growth and saponin accumulation were measured in two lines of transgenic hairy roots of <em>Platycodon grandiflorum</em>, Pl 6 and Pl 17, cultured for 8 weeks in 250-ml shake flasks containing 50 ml of hormone-free woody plant medium supplemented with 40 g/l sucrose and in the Pl 17 line cultured for 12 weeks in a 5-l mist bioreactor containing 1.5 l of the same medium. With both methods, the growth of transgenic hairy roots was assessed as both fresh and dry weight and the biomass growth was correlated with the conductivity and sucrose uptake. The accumulation of saponins was measured and compared with that in roots derived from the field cultivation. The saponin concentrations were significantly higher in the two hairy root lines cultured in shake flasks [6.92 g/100 g d.w. (g%) and 5.82 g% in Pl 6 and Pl 17, respectively] and the line cultured in the bioreactor (5.93 g%) than in the roots derived from the field cultivation (4.02 g%). The results suggest that cultures of <em>P. grandiflorum</em> hairy roots may be a valuable source for obtaining saponins.
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Dissertations / Theses on the topic "Hairy root cultivation"

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Liu, Yi-Sheng, and 劉宜勝. "The Establishment and Cultivation of the Hairy Root of Rehmannia glutinosa." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/60341150863764722729.

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碩士
國立臺灣大學
微生物與生化學研究所
93
Chinese foxglove (Rehmannia glutinosa) is a valuable herb medicine which has been used in traditional Chinese medicine. Hairy root cultures were used as a culture system in vitro for the production of medicinally important compounds to avoid many of the problems that affect the traditional production from field-grown species. We successfully established hairy root cultures of Chinese foxglove by infection with A. rhizogenes. The induction and growth of hairy root were differently affected by basal culture media, the age of plant, plant growth regulators and bacteria. The best induction and growth of hairy roots were obtained on White’s medium with 3% sucrose and 2,4-D (2mg/L). The efficiencies of hairy roots’ induction by A. rhizogenes A4 or ATCC 15834 strain were not different.
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Bhadra, Rajiv. "Establishment, cultivation and optimization of hairy roots of Catharanthus roseus for the synthesis of indole alkaloids." Thesis, 1995. http://hdl.handle.net/1911/19096.

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"Hairy" roots hold the potential for economically feasible biotechnological routes to the controlled biosynthesis of complex, plant-derived, 'natural' molecules. A novel transgenic root system of the tropical plant Catharanthus roseus was established and analyzed for the synthesis of indole alkaloids, including the valuable anti-cancer drugs vinblastine and vincristine. A structured approach to developing the biosynthetic potential of hairy roots is presented: transformation, screening, selection, optimization of culture protocols, and product enhancement. Five hairy root clones with unoptimized doubling times of 3-4 days and vindoline output of 0.005-0.07% dry weight, were screened from 150 transformants. Hairy root morphology likely under the control of rol genes transferred from the Agrobacterium plasmid, was identified as a key determinant of fitness in liquid culture and a target for transgenic design for large-scale bioreactor environments. The hairy root inoculum was optimized and standardized to facilitate the assessment of culture performance under diverse environmental treatments and in process scale-up. The length of the root tip has a dominant effect on growth, uninfluenced by clonal variability. The optimum inoculum is comprised of 5 root tips, each 35-40 mm long, in 50 mL media. Long-term dose-response and transient studies examined heterotrophic and photoheterotrophic carbon regimes. These studies are unique in the metabolic adaptation of cultures, and examined the putatively antagonistic kinetics of nutrient utilisation and secondary metabolite accumulation. The activities of the cathenamine and bisindole alkaloid pathways responded, respectively to high and moderate sucrose concentrations. The cultures were nitrogen limited with 2-4% sucrose in B5/2 salt. Organic acids were excreted in the presence of excess sugars. The ordered assimilation of macronutrients--ammonium, phosphate and nitrate--corroborated by changes of extracellular pH, have important implications on fed-batch strategies. Tabersonine accumulation was growth-associated, while serpentine accumulated in a non-growth manner. Ajmalicine, catharanthine, vindoline, and compounds tentatively identified as vinblastine and vincristine, accumulated optimally in the late-exponential or early-stationary phase. Photoheterotrophic conditions incremented peak biomass by 60-300%, doubling times by 60%, and vindoline levels by an order of magnitude, likely due to the anapleurotic activity of PEPCase and the light induction of nitrate reductase and vindoline synthesis.
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Book chapters on the topic "Hairy root cultivation"

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Srikantan, Chitra, and Smita Srivastava. "Bioreactor Design and Analysis for Large-Scale Plant Cell and Hairy Root Cultivation." In Hairy Roots, 147–82. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2562-5_7.

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Patra, Nivedita, and Ashok K. Srivastava. "Mass Production of Artemisinin Using Hairy Root Cultivation of Artemisia annua in Bioreactor." In Reference Series in Phytochemistry, 343–59. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-54600-1_20.

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Patra, Nivedita, and Ashok K. Srivastava. "Mass Production of Artemisinin Using Hairy Root Cultivation of Artemisia annua in Bioreactor." In Reference Series in Phytochemistry, 1–17. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-32004-5_20-1.

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Thakore, Dhara, and Ashok Kumar Srivastava. "Mass Scale Hairy Root Cultivation of Catharanthus roseus in Bioreactor for Indole Alkaloid Production." In Reference Series in Phytochemistry, 487–502. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-30185-9_21.

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Thakore, Dhara, and Ashok Kumar Srivastava. "Mass Scale Hairy Root Cultivation of Catharanthus roseus in Bioreactor for Indole Alkaloid Production." In Reference Series in Phytochemistry, 1–16. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-11253-0_21-1.

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Mehrotra, Shakti, Sonal Mishra, and Vikas Srivastava. "Bioreactor Technology for Hairy Roots Cultivation." In Reference Series in Phytochemistry, 483–506. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-54600-1_10.

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Mehrotra, Shakti, Sonal Mishra, and Vikas Srivastava. "Bioreactor Technology for Hairy Roots Cultivation." In Bioprocessing of Plant In Vitro Systems, 1–25. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-32004-5_10-1.

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Georgiev, Vasil G., Thomas Bley, and Atanas I. Pavlov. "Bioreactors for the Cultivation of Red Beet Hairy Roots." In Red Beet Biotechnology, 251–81. Boston, MA: Springer US, 2012. http://dx.doi.org/10.1007/978-1-4614-3458-0_11.

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Wilson, Peter D. G. "The Pilot-Scale Cultivation of Transformed Roots." In Hairy Roots, 179–90. CRC Press, 2020. http://dx.doi.org/10.1201/9780367810610-16.

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Conference papers on the topic "Hairy root cultivation"

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VAN, TRAN. "Ri-gene transformation into leaf tissues of Artemisia annua L. and cultivation of hairy-root for artemisinin accumulation." In Seventh International Conference on Advances in Applied Science and Environmental Technology - ASET 2017. Institute of Research Engineers and Doctors, 2017. http://dx.doi.org/10.15224/978-1-63248-136-8-26.

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