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Journal articles on the topic 'GSTO1-1'

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Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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1

Radic, Tanja, Vesna Coric, Zoran Bukumiric, Marija Pljesa-Ercegovac, Tatjana Djukic, Natasa Avramovic, Marija Matic, et al. "GSTO1*CC Genotype (rs4925) Predicts Shorter Survival in Clear Cell Renal Cell Carcinoma Male Patients." Cancers 11, no. 12 (December 17, 2019): 2038. http://dx.doi.org/10.3390/cancers11122038.

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Omega class glutathione transferases, GSTO1-1 and GSTO2-2, exhibit different activities involved in regulation of inflammation, apoptosis and redox homeostasis. We investigated the the prognostic significance of GSTO1 (rs4925) and GSTO2 (rs156697 and rs2297235) polymorphisms in clear cell renal cell carcinoma (ccRCC) patients. GSTO1-1 and GSTO2-2 expression and phosphorylation status of phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/ /mammalian target of rapamycin (mTOR) and Raf/MEK/extracellular signal-regulated kinase (ERK) signaling pathways in non-tumor and tumor ccRCC tissue, as well as possible association of GSTO1-1 with signaling molecules were also assessed. GSTO genotyping was performed by quantitative PCR in 228 ccRCC patients, while expression and immunoprecipitation were analyzed by Western blot in 30 tissue specimens. Shorter survival in male carriers of GSTO1*C/C wild-type genotype compared to the carriers of at least one variant allele was demonstrated (p = 0.049). GSTO1*C/C genotype independently predicted higher risk of overall mortality among male ccRCC patients (p = 0.037). Increased expression of GSTO1-1 and GSTO2-2 was demonstrated in tumor compared to corresponding non-tumor tissue (p = 0.002, p = 0.007, respectively), while GSTO1 expression was correlated with interleukin-1β (IL-1β)/pro-interleukin-1β (pro-IL-1β) ratio (r = 0.260, p = 0.350). Interaction of GSTO1 with downstream effectors of investigated pathways was shown in ccRCC tumor tissue. This study demonstrated significant prognostic role of GSTO1 polymorphism in ccRCC. Up-regulated GSTO1-1 and GSTO2-2 in tumor tissue might contribute to aberrant ccRCC redox homeostasis.
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2

Yin, Zhan-Li, Jane E. Dahlstrom, David G. Le Couteur, and Philip G. Board. "Immunohistochemistry of Omega Class Glutathione S-Transferase in Human Tissues." Journal of Histochemistry & Cytochemistry 49, no. 8 (August 2001): 983–87. http://dx.doi.org/10.1177/002215540104900806.

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Omega class glutathione transferase (GSTO) has been recently described in a number of mammalian species. We used immunohistochemistry to determine the cellular and tissue distribution of GSTO1–1 in humans. Expression of GSTO1–1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells. We also found nuclear staining in several types of cells, including glial cells, myoepithelial cells of the breast, neuroendocrine cells of colon, fetal myocytes, hepatocytes, biliary epithelium, ductal epithelium of the pancreas, Hoffbauer cells of the placenta, and follicular and C-cells of the thyroid. These observations and the known activity of GSTO1–1 suggest biological functions that are not shared with other GSTs. (J Histochem Cytochem 49:983–987, 2001)
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3

Tummala, Padmaja, Melissa Rooke, Jane E. Dahlstrom, Shuhei Takahashi, Marco G. Casarotto, Nilisha Fernando, Mark M. Hughes, Luke A. J. O’Neill, and Philip G. Board. "Glutathione transferase Omega 1 confers protection against azoxymethane-induced colorectal tumour formation." Carcinogenesis 42, no. 6 (February 10, 2021): 853–63. http://dx.doi.org/10.1093/carcin/bgab008.

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Abstract Inflammatory bowel disease (IBD) is characterized by multiple alterations in cytokine expression and is a risk factor for colon cancer. The Omega class glutathione transferase GSTO1-1 regulates the release of the pro-inflammatory cytokines interleukin 1β (IL-1β) and interleukin 18 (IL-18) by deglutathionylating NEK7 in the NLRP3 inflammasome. When treated with azoxymethane and dextran sodium sulphate (AOM/DSS) as a model of IBD, Gsto1−/− mice were highly sensitive to colitis and showed a significant increase in the size and number of colon tumours compared with wild-type (WT) mice. Gsto1−/− mice treated with AOM/DSS had significantly lower serum IL-1β and IL-18 levels as well as significantly decreased interferon (IFN)-γ, decreased pSTAT1 and increased pSTAT3 levels in the distal colon compared with similarly treated WT mice. Histologically, AOM/DSS treated Gsto1−/− mice showed increased active chronic inflammation with macrophage infiltration, epithelial dysplasia and invasive adenocarcinoma compared with AOM/DSS treated WT mice. Thus, this study shows that GSTO1-1 regulates IL-1β and IL-18 activation and protects against colorectal cancer formation in the AOM/DSS model of IBD. The data suggest that while GSTO1-1 is a new target for the regulation of the NLRP3 inflammasome-associated cytokines IL-1β and IL-18 by small molecule inhibitors, there is a possibility that anti-inflammatory drugs targeting these cytokines may potentiate colon cancer in some situations.
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4

Ada, Tugba Guzide, Ahmet Oguz Ada, Semih Celalettin Kunak, Sibel Alpar, Meral Gulhan, and Mumtaz Iscan. "Association Between Glutathione S-Transferase Omega 1 A140D Polymorphism in the Turkish Population and Susceptibility to Non-Small Cell Lung Cancer." Archives of Industrial Hygiene and Toxicology 64, no. 2 (June 1, 2013): 247–53. http://dx.doi.org/10.2478/10004-1254-64-2013-2302.

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Recent years have seen a growing evidence of ethnic differences in the frequency of glutathione S-transferase omega 1 (GSTO1) A140D gene polymorphism, which is associated with various cancers such as breast and liver. Until now however, no association has been investigated between the GSTO1 A140D polymorphism and lung cancer. The aim of our study was to see if there was one in the Turkish population. To do that, we identified GSTO1 A140D polymorphism in 214 unrelated healthy individuals and 172 patients with non-small cell lung cancer (NSCLC) using the polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method. The frequencies of A/A (wild type), A/D (heterozygous mutant), and D/D (homozygous mutant) GSTO1 A140D genotypes in healthy subjects were 48 %, 41 %, and 11 %, respectively. In NSCLC patients they were 48 %, 45 %, and 7 %, respectively. We found no significant association between the GSTO1 A140D gene polymorphism and NSCLC or its histological subtypes, namely squamous cell carcinoma or adenocarcinoma. Furthermore, this polymorphism did not correlate with smoking. Our study is the first to show that the frequency of GSTO1 A140D gene polymorphism in the Turkish population is similar to other Caucasian populations and that this polymorphism is not associated with susceptibility to NSCLC.
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5

Tạ Thị Bình, Tạ Thị Bình Tạ Thị Bình, Trần Phương Thảo, Nguyễn Khắc Hải, and Nguyễn Huy Hoàng. "The association between gsto1 polymorphisms and arsenic methylation of prenatal arsenic exposed infants." Vietnam Journal of Biotechnology 15, no. 2 (April 20, 2018): 223–30. http://dx.doi.org/10.15625/1811-4989/15/2/12337.

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The trace element arsenic naturally presents in the environment. Arsenic is the essential factor to the human body at low level, however it causes environmental pollution and have negative effects to health at high level. Recently, arsenic contamination as well as its effects on public health, especially infants and children is increasingly becoming important and serious issues in worldwide. Glutathione S-transferase omega-1 (GSTO1) is a phase II enzymatic detoxification of xenobiotics in variety of animals including humans; to catalyze the arsenic methylation. The difference of urinary arsenic component in each individual may relate to the genetic polymorphism. To evaluate the variations of single nucleotide polymorphisms of GSTO1, PCR-RFLP technology was ultilized. Single nucleotide polymorphisms (SNPs) genotype of 150 cohort blood samples at GSTO1 Thr->Asn (rs15032), GSTO1 Ala->Val (rs11509439) and GSTO1 Ala->Asp (rs4925) were detected. The association between GSTO1 polymorphisms and prenatal arsenic exposure was evaluated by statistical analysis such as SPSS software version 20, t-test and oneway ANOVA. The results showed that GSTO1 Ala->Asp (rs4925) was statistically associated with MMA/iAs (p = 0.041). Differences between ratio of MMA/iAs and genotypes were checked by Tukey-Kramer method, along with oneway ANOVA showed that Individuals taking the AA genotype had higher MMA/ iAs ratio than individuals carrying the CC genotype, with a statistically significant association (p = 0.044), also clearly higher than the individuals carrying AC genotype, significant at p = 0.046. Therefore, it is possible that individuals carrying the AA genotype in the polymorphism have higher arsenic excretion than individuals with CC and AC genotypes
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6

Menon, Deepthi, and Philip G. Board. "A Role for Glutathione Transferase Omega 1 (GSTO1-1) in the Glutathionylation Cycle." Journal of Biological Chemistry 288, no. 36 (July 25, 2013): 25769–79. http://dx.doi.org/10.1074/jbc.m113.487785.

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7

Fernando, Nilisha, Yvette Wooff, Riemke Aggio-Bruce, Joshua A. Chu-Tan, Haihan Jiao, Catherine Dietrich, Matt Rutar, et al. "Photoreceptor Survival Is Regulated by GSTO1-1 in the Degenerating Retina." Investigative Opthalmology & Visual Science 59, no. 11 (September 4, 2018): 4362. http://dx.doi.org/10.1167/iovs.18-24627.

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8

Menon, D., R. Coll, L. A. J. O'Neill, and P. G. Board. "GSTO1-1 modulates metabolism in macrophages activated through the LPS and TLR4 pathway." Journal of Cell Science 128, no. 10 (April 23, 2015): 1982–90. http://dx.doi.org/10.1242/jcs.167858.

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9

Piaggi, S., C. Raggi, A. Corti, E. Pitzalis, M. C. Mascherpa, M. Saviozzi, A. Pompella, and A. F. Casini. "Glutathione transferase omega 1-1 (GSTO1-1) plays an anti-apoptotic role in cell resistance to cisplatin toxicity." Carcinogenesis 31, no. 5 (January 27, 2010): 804–11. http://dx.doi.org/10.1093/carcin/bgq031.

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10

Oakley, Aaron J. "Proposed mechanism for monomethylarsonate reductase activity of human omega-class glutathione transferase GSTO1-1." Biochemical and Biophysical Research Communications 590 (January 2022): 7–13. http://dx.doi.org/10.1016/j.bbrc.2021.12.072.

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11

Saisawang, Chonticha, Jantana Wongsantichon, Robert C. Robinson, and Albert J. Ketterman. "Glutathione transferase Omega 1‐1 (GSTO1‐1) modulates Akt and MEK1/2 signaling in human neuroblastoma cell SH‐SY5Y." Proteins: Structure, Function, and Bioinformatics 87, no. 7 (March 25, 2019): 588–95. http://dx.doi.org/10.1002/prot.25683.

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12

Sokulsky, Leon A., Bridie Goggins, Simonne Sherwin, Fiona Eyers, Gerard E. Kaiko, Philip G. Board, Simon Keely, Ming Yang, and Paul S. Foster. "GSTO1‐1 is an upstream suppressor of M2 macrophage skewing and HIF‐1α‐induced eosinophilic airway inflammation." Clinical & Experimental Allergy 50, no. 5 (March 2, 2020): 609–24. http://dx.doi.org/10.1111/cea.13582.

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13

Paul, Souren, Rekha Jakhar, Monika Bhardwaj, and Sun Chul Kang. "Glutathione-S-transferase omega 1 (GSTO1-1) acts as mediator of signaling pathways involved in aflatoxin B1-induced apoptosis–autophagy crosstalk in macrophages." Free Radical Biology and Medicine 89 (December 2015): 1218–30. http://dx.doi.org/10.1016/j.freeradbiomed.2015.11.006.

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14

Sato, Risa, Kotaro Ohmori, Mina Umetsu, Masaki Takao, Mitsutoshi Tano, Gerald Grant, Brenda Porter, Anthony Bet, Tetsuya Terasaki, and Yasuo Uchida. "An Atlas of the Quantitative Protein Expression of Anti-Epileptic-Drug Transporters, Metabolizing Enzymes and Tight Junctions at the Blood–Brain Barrier in Epileptic Patients." Pharmaceutics 13, no. 12 (December 9, 2021): 2122. http://dx.doi.org/10.3390/pharmaceutics13122122.

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The purpose of the present study was to quantitatively elucidate the levels of protein expression of anti-epileptic-drug (AED) transporters, metabolizing enzymes and tight junction molecules at the blood–brain barrier (BBB) in the focal site of epilepsy patients using accurate SWATH (sequential window acquisition of all theoretical fragment ion spectra) proteomics. Brain capillaries were isolated from focal sites in six epilepsy patients and five normal brains; tryptic digests were produced and subjected to SWATH analysis. MDR1 and BCRP were significantly downregulated in the epilepsy group compared to the normal group. Out of 16 AED-metabolizing enzymes detected, the protein expression levels of GSTP1, GSTO1, CYP2E1, ALDH1A1, ALDH6A1, ALDH7A1, ALDH9A1 and ADH5 were significantly 2.13-, 6.23-, 2.16-, 2.80-, 1.73-, 1.67-, 2.47- and 2.23-fold greater in the brain capillaries of epileptic patients than those of normal brains, respectively. The protein expression levels of Claudin-5, ZO-1, Catenin alpha-1, beta-1 and delta-1 were significantly lower, 1.97-, 2.51-, 2.44-, 1.90- and 1.63-fold, in the brain capillaries of epileptic patients compared to those of normal brains, respectively. Consistent with these observations, leakage of blood proteins was also observed. These results provide for a better understanding of the therapeutic effect of AEDs and molecular mechanisms of AED resistance in epileptic patients.
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15

Li, Yang, Qi Zhang, Bo Peng, Qing Shao, Wei Qian, and Jian-Ying Zhang. "Identification of glutathione S-transferase omega 1 (GSTO1) protein as a novel tumor-associated antigen and its autoantibody in human esophageal squamous cell carcinoma." Tumor Biology 35, no. 11 (August 2, 2014): 10871–77. http://dx.doi.org/10.1007/s13277-014-2394-y.

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16

Lee, Jae-Min, Joo-Hee Lee, Min-Kyung Song, and Youn-Jung Kim. "NXP031 Improves Cognitive Impairment in a Chronic Cerebral Hypoperfusion-Induced Vascular Dementia Rat Model through Nrf2 Signaling." International Journal of Molecular Sciences 22, no. 12 (June 11, 2021): 6285. http://dx.doi.org/10.3390/ijms22126285.

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Vascular dementia (VaD) is a progressive cognitive impairment caused by a reduced blood supply to the brain. Chronic cerebral hypoperfusion (CCH) is one cause of VaD; it induces oxidative stress, neuroinflammation, and blood-brain barrier (BBB) disruption, damaging several brain regions. Vitamin C plays a vital role in preventing oxidative stress-related diseases induced by reactive oxygen species, but it is easily oxidized and loses its antioxidant activity. To overcome this weakness, we have developed a vitamin C/DNA aptamer complex (NXP031) that increases vitamin C’s antioxidant efficacy. Aptamers are short single-stranded nucleic acid polymers (DNA or RNA) that can interact with their corresponding target with high affinity. We established an animal model of VaD by permanent bilateral common carotid artery occlusion (BCCAO) in 12 week old Wistar rats. Twelve weeks after BCCAO, we injected NXP031 into the rats intraperitoneally for two weeks at moderate (200 mg/4 mg/kg) and high concentrations (200 mg/20 mg/kg). NXP031 administration alleviates cognitive impairment, microglial activity, and oxidative stress after CCH. NXP031 increased the expression of basal lamina (laminin), endothelial cell (RECA-1, PECAM-1), and pericyte (PDGFRβ); these markers maintain the BBB integrity. We found that NXP031 administration activated the Nrf2-ARE pathway and increased the expression of SOD-1 and GSTO1/2. These results suggest that this new aptamer complex, NXP031, could be a therapeutic intervention in CCH-induced VaD.
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17

Sun, Desheng, Rong Lin, and Yao Ouyang. "The Role of CD40, CD86, and Glutathione S-Transferase Omega 1 in the Pathogenesis of Chronic Obstructive Pulmonary Disease." Canadian Respiratory Journal 2022 (August 23, 2022): 1–7. http://dx.doi.org/10.1155/2022/6810745.

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Objective. The aim of the study was to explore the relevance of CD40, CD86, and GSTO1 with the pathogenesis of COPD. Methods. Patients with acute exacerbation of COPD were contrasted with the healthy and nonsmoking ones and smoking but without COPD ones. The changes of CD40, CD86, and GSTO1 in the peripheral blood, collected from different groups, were detected by flow cytometry and western blotting, respectively. Results. Compared with the nonsmoking group and smoking but without the COPD group, the expression of CD40 and CD86 of the patients with COPD increased significantly, but the expression of GSTO1 decreased. CD40 and CD86 were negatively correlated with FEV1%, while GSTO1 was positively correlated with FEV1% and negatively correlated with CD40 and CD86. Conclusion. CD40, CD86, and GSTO1 may play a role in the pathogenesis of COPD, and they are related to the severity of COPD and the degree of changes in the lung function.
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Lee, Jae-Min, Joo Hee Lee, Min Kyung Song, and Youn-Jung Kim. "NXP032 Ameliorates Aging-Induced Oxidative Stress and Cognitive Impairment in Mice through Activation of Nrf2 Signaling." Antioxidants 11, no. 1 (January 7, 2022): 130. http://dx.doi.org/10.3390/antiox11010130.

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Aging is a neurodegenerative disease that leads to cognitive impairment, and an increase in oxidative stress as a major cause is an important factor. It has been reported that aging-related cognitive impairment is associated with increased oxidative damage in several brain regions during aging. As a powerful antioxidant, vitamin C plays an important role in preventing oxidative stress, but due to its unstable chemical properties, it is easily oxidized and thus the activity of antioxidants is reduced. In order to overcome this easily oxidized vulnerability, we developed NXP032 (vitamin C/DNA aptamer complex) that can enhance the antioxidant efficacy of vitamin C using an aptamer. We developed NXP032 (vitamin C/DNA Aptamin C320 complex) that can enhance the antioxidant efficacy of vitamin C using an aptamer. In the present study, we evaluated the neuroprotective effects of NXP032 on aging-induced cognitive decline, oxidative stress, and neuronal damage in 17-month-old female mice. NXP032 was orally administered at 200 mg/kg of ascorbic acid and 4 mg/kg of DNA aptamer daily for eight weeks. Before the sacrifice, a cognitive behavioral test was performed. Administration of NXP032 alleviated cognitive impairment, neuronal damage, microglia activity, and oxidative stress due to aging. We found that although aging decreases the Nrf2-ARE pathway, NXP032 administration activates the Nrf2-ARE pathway to increase the expression of SOD-1 and GSTO1/2. The results suggest that the new aptamer complex NXP032 may be a therapeutic intervention to alleviate aging-induced cognitive impairment and oxidative stress.
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19

Seo, Mi Hyun, Dae Won Kim, Yeon Sook Kim, and Suk Keun Lee. "Pentoxifylline-induced protein expression change in RAW 264.7 cells as determined by immunoprecipitation-based high performance liquid chromatography." PLOS ONE 17, no. 3 (March 25, 2022): e0261797. http://dx.doi.org/10.1371/journal.pone.0261797.

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Although pentoxifylline (PTX) was identified as a competitive non-selective phosphodiesterase inhibitor, its pharmacological effect has not been clearly elucidated. The present study explored the effect of low dose 10 μg/mL PTX (therapeutic dose) compared to high dose 300 μg/mL PTX (experimental dose) in RAW 264.7 cells through immunoprecipitation-based high performance liquid chromatography (IP-HPLC), immunohistochemistry, and western blot. 10 μg/mL PTX increased the expression of proliferation (Ki-67, PCNA, cyclin D2, cdc25A), epigenetic modification (KDM4D, PCAF, HMGB1), protein translation (DOHH, DHPS, eIF5A1), RAS signaling (KRAS, pAKT1/2/3, PI3K), NFkB signaling (NFkB, GADD45, p38), protection (HSP70, SOD1, GSTO1/2), survival (pAKT1/2/3, SP1, sirtuin 6), neuromuscular differentiation (NSEγ, myosin-1a, desmin), osteoblastic differentiation (BMP2, RUNX2, osterix), acute inflammation (TNFα, IL-1, CXCR4), innate immunity (β-defensin 1, lactoferrin, TLR-3, -4), cell-mediated immunity (CD4, CD8, CD80), while decreased the expression of ER stress (eIF2α, eIF2AK3, ATF6α), fibrosis (FGF2, CTGF, collagen 3A1), and chronic inflammation (CD68, MMP-2, -3, COX2) versus the untreated controls. The activation of proliferation by 10 μg/mL PTX was also supported by the increase of cMyc-MAX heterodimer and β-catenin-TCF1 complex in double IP-HPLC. 10 μg/mL PTX enhanced FAS-mediated apoptosis but diminished p53-mediated apoptosis, and downregulated many angiogenesis proteins (angiogenin, VEGF-A, and FLT4), but upregulated HIF1α, VEGFR2, and CMG2 reactively. Whereas, 300 μg/mL PTX consistently decreased proliferation, epigenetic modification, RAS and NFkB signaling, neuromuscular and osteoblastic differentiation, but increased apoptosis, ER stress, and fibrosis compared to 10 μg/mL PTX. These data suggest PTX has different biological effect on RWA 264.7 cells depending on the concentration of 10 μg/mL and 300 μg/mL PTX. The low dose 10 μg/mL PTX enhanced RAS/NFkB signaling, proliferation, differentiation, and inflammation, particularly, it stimulated neuromuscular and osteoblastic differentiation, innate immunity, and cell-mediated immunity, but attenuated ER stress, fibrosis, angiogenesis, and chronic inflammation, while the high dose 300 μg/mL PTX was found to alleviate the 10 μg/mL PTX-induced biological effects, resulted in the suppression of RAS/NFkB signaling, proliferation, neuromuscular and osteoblastic differentiation, and inflammation.
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20

Sumiya, Ryusuke, Masayoshi Terayama, Teruki Hagiwara, Kazuaki Nakata, Satoshi Nagasaka, Kazuhiko Yamada, Norihiro Kokudo, Hiromu Suzuki, and Kawamura I. Yuki. "Abstract 3783: GSTO2, a novel tumor suppressor gene of lung squamous cell carcinoma, regulates mitochondria function via the p38/β-catenin signaling pathway." Cancer Research 82, no. 12_Supplement (June 15, 2022): 3783. http://dx.doi.org/10.1158/1538-7445.am2022-3783.

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Abstract Glutathione S-transferase omega 2 (GSTO2) is one of regulators of GSH/GSSG balance, and its polymorphism showed strong associations with lung functions as well as the risk of chronic obstructive pulmonary disease. Recently, we found that GSTO2 was exclusively expressed in airway basal cells, Clara cells and type II alveolar cells, which have self-renewal capacity in the lungs; however, its expression was lost in lung squamous cell carcinoma (LSCC). In the present study, we restored GSTO2 expression in LSCC cell lines (LK-2 and H520) to clarify the significance of GSTO2 loss in LSCC. In both LSCC cell lines used, GSTO2 overexpression significantly inhibited cell growth and colony formation in vitro. In a subcutaneous xenograft model, GSTO2-transfected LK-2 cells formed smaller tumors in nude mice than mock-transfected cells. Upon intravenous injection into nude mice, the incidence of liver metastasis was lower in mice injected with GSTO2-transfected LK-2 cells than in those injected with mock-transfected cells. Metabolomic analyses using the XF96 extracellular flux analyzer revealed that GSTO2 overexpression suppressed mitochondrial oxidative phosphorylation but did not affect glycolysis. Upon JC-1 dye staining, GSTO2-transfected cells showed decreased mitochondrial membrane potential compared with mock-transfected cells. Since β-catenin has been reported as a novel regulator of the OXPHOS in hepatocytes, we next examined the effect of GSTO2 expression on β-catenin expression in LSCC and found that GSTO2 overexpression suppressed the expression of β-catenin. Because p38 phosphorylation was accelerated in both GSTO2-transfected cells, we examined the involvement of the p38 signaling pathway in the GSTO2-mediated downregulation of β-catenin as well as mitochondrial membrane potential in LSCC.When GSTO2-transfected cells were treated with SB203580, a specific inhibitor of p38 MAPK, β-catenin expression and mitochondrial membrane potentialwere restored. Finally, we examine whether DNA methylation of the GSTO2 could explain the loss of GSTO2 expression in LSCC. When human LSCC cell lines were treated with 5-aza-2′-deoxycytidine, a DNA-methyltransferase inhibitor, GSTO2 transcription was induced. Bisulfite sequencing showed that the promoter region of the GSTO2 was frequently methylated in LSCC tissues than that of normal tissue. Our study indicated that the loss of GSTO2 via DNA hypermethylation contributes to the cell growth and progression of LSCC, probably by modulating oxidative phosphorylation in mitochondria via the p38/β-catenin signaling pathway. Citation Format: Ryusuke Sumiya, Masayoshi Terayama, Teruki Hagiwara, Kazuaki Nakata, Satoshi Nagasaka, Kazuhiko Yamada, Norihiro Kokudo, Hiromu Suzuki, Kawamura I. Yuki. GSTO2, a novel tumor suppressor gene of lung squamous cell carcinoma, regulates mitochondria function via the p38/β-catenin signaling pathway [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 3783.
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21

Szymańska-Chabowska, A., T. Matys, Ł. Łaczmański, K. Czerwińska, A. Janus, B. Smyk, G. Mazur, R. Poręba, and P. Gać. "The relationship between PNP, GSTO-1, AS3MT and ADRB3 gene polymorphisms and urinary arsenic concentration among copper smelter and refinery employers." Human & Experimental Toxicology 39, no. 11 (May 26, 2020): 1443–53. http://dx.doi.org/10.1177/0960327120925891.

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Introduction: The aim of this study was to assess the relationship between polymorphisms of genes encoding enzymes involved in arsenic metabolism and urinary arsenic concentration in people occupationally exposed to arsenic. Materials and Methods: The data from 113 employers directly exposed to lead, cadmium, and arsenic in copper smelter in Legnica and Glogow were collected. Urinary arsenic concentration was measured. In addition, blood level of cadmium, lead, and zinc protoporphyrins was assayed. Genetic analyses included polymorphism of PNP (rs 1130650), GSTO-1 (rs 4925), AS3MT (rs 11191439), and ADRB3 (rs4994) genes. Results: Individuals occupationally exposed to arsenic compounds, who have allele T in homozygous constellation in locus rs 1130650 of PNP gene, are predisposed to lower urinary arsenic concentration, while AA homozygosity in locus rs 4925 of GSTO-1 gene may result in statistically significant higher urinary arsenic concentration. Polymorphisms of AS3MT and ADRB3 genes showed no statistically significant correlation with urinary arsenic, however, there was a tendency to higher arsenic concentration in allele A carriers in locus rs4994 of ADRB3 gene and in allele T carriers in rs 11191439 of AS3MT gene. Conclusion: This study indicates that arsenic absorption and metabolism depend on polymorphisms of genes encoding PNP and GSTO-1. Individuals with disadvantageous constellation of polymorphisms are more susceptible to harmful effects of arsenic exposure.
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Tanaka-Kagawa, Toshiko, Hideto Jinno, Tatsuya Hasegawa, Yuko Makino, Yoshiyuki Seko, Nobumitsu Hanioka, and Masanori Ando. "Functional characterization of two variant human GSTO 1-1s (Ala140Asp and Thr217Asn)." Biochemical and Biophysical Research Communications 301, no. 2 (February 2003): 516–20. http://dx.doi.org/10.1016/s0006-291x(02)03066-8.

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23

Burmeister, Cora, Kai LÜersen, Alexander Heinick, Ayman Hussein, Marzena Domagalski, Rolf D. Walter, and Eva Liebau. "Oxidative stress in Caenorhabditis elegans : protective effects of the Omega class glutathione transferase ( GSTO‐1 )." FASEB Journal 22, no. 2 (September 27, 2007): 343–54. http://dx.doi.org/10.1096/fj.06-7426com.

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24

Zhang, Junnan, Jiajing Chen, Jing Yang, Sijia Gong, Jiangxia Zheng, and Guiyun Xu. "Effects of Lard and Vegetable Oils Supplementation Quality and Concentration on Laying Performance, Egg Quality and Liver Antioxidant Genes Expression in Hy-Line Brown." Animals 11, no. 3 (March 10, 2021): 769. http://dx.doi.org/10.3390/ani11030769.

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This study examined the effects of various types, quality, and levels of dietary oils on laying performance and the expression patterns of antioxidant-related genes in Hy-line brown laying hens. A total of 720 40-week-old Hy-line brown laying hens were fed the same corn-soybean basal meals but containing 0.5 or 1.5% normal or oxidized soybean oil or lard, a total of 8 treatments. The results showed that laying rate (LR) and fatty acids of raw yolk were significantly correlated dietary type of oil (p < 0.05). With the increasing concentration of normal oil, it significantly increased LR and decreased feed conversion ratio (FCR, feed/egg) and albumen height of laying hens. The oxidized oil significant decreased the production performance of laying hens; and adding 1.5% of oxidized lard into feeds could destroy the integrity of yolk spheres of cooked yolk. mRNA expression of liver antioxidant-related genes increased when dietary oxidized oils were added into feeds. By comparing different qualities oil effect on antioxidant-related genes, the expression of Glutathione S-Transferase Theta 1 (GSTT1), Glutathione S-Transferase Alpha 3 (GSTA3), Glutathione S-Transferase Omega 2 (GSTO2), and Superoxide Dismutase 2 (SOD2) were increased when dietary oils were oxidized, in which change of the GSTO2 expression was the most with 1.5% of oxidized soybean oil. In conclusion, the ideal type of oil for Hy-line brown layer hens is soybean comparing with lard in a corn-soybean diet, avoiding using of oxidized oil.
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Успанова, А. С., and С. А. Алиев. "APPLICATION OF BIM TECHNOLOGIES IN THE RECONSTRUCTION OF THE 1-st FLOOR HALL OF THE GSTOU BUILDING No. 2." Вестник ГГНТУ. Технические науки, no. 4(26) (December 28, 2021): 73–79. http://dx.doi.org/10.34708/gstou.2021.63.20.010.

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Современное проектирование невозможно представить без программных комплексов, призванных повысить эффективность строительного производства. Технологии ВIM в строительной отрасли привлекают все больше пользователей ввиду их универсальности, возможности адаптации к любым видам проектных работ в строительстве, больших возможностей интеграции с другими участниками проекта. ГГГНТУ им. акад. М. Д. Миллионщикова ведет активную работу по внедрению в учебный процесс программных продуктов Archicad, Autodesk 3ds Max, Revit для подготовки специалистов, обладающих навыками работы в данных программах. Применение современных ВIM не только в образовательном процессе, но и на практике дает возможность более тесного ознакомления инструментами данных программ. В данной статье рассмотрены возможности применения ВIM в ходе реконструкции холла первого этажа второго учебного корпуса Университета, позволившие выявить наиболее оптимальные методы проектирования в целях повышения качества производства работ. Modern design cannot be imagined without modern application programs designed for the efficiency of construction production. BIM technologies in the construction industry are attracting more and more users due to their versatility, the ability to adapt to any type of design work in construction, and great opportunities for integration with other project participants.GSTOU them. acad. M. D. Millionshchikova is actively working on introducing Archicad, Autodesk 3ds Max, Revit software products into the educational process to train specialists with the skills to work in these programs. The use of modern VIM not only in the educational process, but also in practice makes it possible to better familiarize with the tools of these programs. This article discusses the possibilities of using BIM during the reconstruction of the hall of the first floor of the second academic building of the University, which made it possible to identify the most optimal design methods in order to improve the quality of work.
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Liu, Jing, Zhiying Sun, Zhi Wang, and Yuande Peng. "A Comparative Transcriptomics Approach to Analyzing the Differences in Cold Resistance in Pomacea canaliculata between Guangdong and Hunan." Journal of Immunology Research 2020 (August 3, 2020): 1–9. http://dx.doi.org/10.1155/2020/8025140.

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Pomacea canaliculata, known as an invasive freshwater snail, is also called a golden apple snail; its survival and expansion are greatly affected by temperature. In this study, high-throughput sequencing (RNA-seq) was used to perform comparative transcriptome analysis on the muscular tissue (G_M) of snails in Guangdong and Hunan. Differential gene screening was performed with FDR <0.05 and |log2FoldChange| >1 as the threshold, and a total of 1,368 differential genes were obtained (671 genes showed upregulation in snails from Guangdong, and 697 genes displayed upregulation in snails from Hunan). Fifteen genes were identified as candidate genes for the cold hardiness of Pomacea canaliculata. Among them, three genes were involved in energy metabolism (glycogen synthase, 1; DGK, 1; G6PD, 1); seven genes were involved in homeostasis regulation (HSP70, 2; BIP, 1; GPX, 1; GSTO 1, G6PD, 1; caspase-9, 1); two genes were involved in amino acid metabolism (glutamine synthetase, 1; PDK, 1); and four genes were involved in membrane metabolism (inositol-3-phosphate synthase, 1; Na+/K+-ATPase, 1; calcium-binding protein, 2). This study presents the molecular mechanisms for the cold hardiness of Pomacea canaliculata, which could provide a scientific basis for the forecast and prevention of harm from Pomacea canaliculata.
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Laroche-Joubert, Nicolas, Sophie Marsy, Stéphanie Luriau, Martine Imbert-Teboul, and Alain Doucet. "Mechanism of activation of ERK and H-K-ATPase by isoproterenol in rat cortical collecting duct." American Journal of Physiology-Renal Physiology 284, no. 5 (May 1, 2003): F948—F954. http://dx.doi.org/10.1152/ajprenal.00394.2002.

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Isoproterenol stimulates H-K-ATPase activity in rat cortical collecting duct β-intercalated cells through a PKA-dependent pathway. This study aimed at determining the signaling pathway underlying this effect. H-K-ATPase activity was determined in microdissected collecting ducts preincubated with or without specific inhibitors or antibodies against intracellular signaling proteins. Transient cell membrane permeabilization with streptolysin-O allowed intracellular access to antibodies. Isoproterenol increased phosphorylation of ERK in a PKA-dependent manner, and inhibition of the ERK phosphorylation prevented the stimulation of H-K-ATPase. Antibodies against the monomeric G protein Ras or the kinase Raf-1 curtailed the stimulation of H-K-ATPase by isoproterenol, whereas antibodies against the related proteins Rap-1 and B-Raf had no effect. Pertussis toxin and inhibition of tyrosine kinases with genistein also curtailed isoproterenol-induced stimulation of H-K-ATPase. It is proposed that activation of PKA by isoproterenol induces the phosphorylation of β-adrenergic receptors and the switch from Gsto Gicoupling. In turn, βγ-subunits released from Giwould activate a tyrosine kinase-Ras-Raf-1 pathway, leading to the activation of ERK1/2 and of H-K-ATPase.
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Garcerá, Ana, Lina Barreto, Lidia Piedrafita, Jordi Tamarit, and Enrique Herrero. "Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases." Biochemical Journal 398, no. 2 (August 15, 2006): 187–96. http://dx.doi.org/10.1042/bj20060034.

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The Saccharomyces cerevisiae genome encodes three proteins that display similarities with human GSTOs (Omega class glutathione S-transferases) hGSTO1-1 and hGSTO2-2. The three yeast proteins have been named Gto1, Gto2 and Gto3, and their purified recombinant forms are active as thiol transferases (glutaredoxins) against HED (β-hydroxyethyl disulphide), as dehydroascorbate reductases and as dimethylarsinic acid reductases, while they are not active against the standard GST substrate CDNB (1-chloro-2,4-dinitrobenzene). Their glutaredoxin activity is also detectable in yeast cell extracts. The enzyme activity characteristics of the Gto proteins contrast with those of another yeast GST, Gtt1. The latter is active against CDNB and also displays glutathione peroxidase activity against organic hydroperoxides such as cumene hydroperoxide, but is not active as a thiol transferase. Analysis of point mutants derived from wild-type Gto2 indicates that, among the three cysteine residues of the molecule, only the residue at position 46 is required for the glutaredoxin activity. This indicates that the thiol transferase acts through a monothiol mechanism. Replacing the active site of the yeast monothiol glutaredoxin Grx5 with the proposed Gto2 active site containing Cys46 allows Grx5 to retain some activity against HED. Therefore the residues adjacent to the respective active cysteine residues in Gto2 and Grx5 are important determinants for the thiol transferase activity against small disulphide-containing molecules.
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Azizian-Farsani, Fatemeh, Gholamreza Rafiei, and Mostafa Saadat. "Impact of Sodium Arsenite on Chromosomal Aberrations With Respect to Polymorphisms of Detoxification and DNA Repair Genes." International Journal of Toxicology 33, no. 6 (November 2014): 518–22. http://dx.doi.org/10.1177/1091581814557953.

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Arsenic compounds can increase production of reactive oxygen species. Reactive oxygen species can induce double-strand breaks in DNA, which is a cause of chromosome aberrations (CAs). This study was conducted to determine the association between arsenic exposure and polymorphisms of genes involved in detoxification (glutathione S-transferase T1 [ GSTT1], glutathione S-transferase M1 [ GSTM1], glutathione S-transferase O2 [ GSTO2], catalase [ CAT], and NAD(P)H quinone oxidoreductase1 [ NQO1]) as well as nonhomologous end joining DNA repair genes ( XRCC4, XRCC5, and XRCC6) with induction of chromosomal aberrations. The participants consisted of 123 healthy males who were genotyped using polymerase chain reaction-based methods. Primary cultures of whole blood were treated with sodium arsenite (NaAsO2; iAs(III); at final concentration 1 µmol/L), mitomycin C (at final concentration 60 ηg/mL; as positive control), or untreated. For each culture, mitotic index (MI), chromatid breaks (CBs), CAs, and total percentage of aberrant cells were determined. The levels of CB and percentage of aberrant cells were significantly higher in the TT genotype of CAT (C-262T polymorphism) than the CC genotype. The CB value in samples with GSTM1 active genotype was significantly higher than the null genotype. The MI in samples with TT genotype of NQO1 (C609T polymorphism) was significantly higher than MI in samples having CC and CT genotypes. There was no association between MI, CB, CA, and percentage of aberrant cells and polymorphisms of XRCC4, XRCC5, and XRCC6.
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Wu, Tao. "PSVIII-18 Dietary supplementation with trihexanoin enhances intestinal function of weaned piglets." Journal of Animal Science 97, Supplement_3 (December 2019): 302–3. http://dx.doi.org/10.1093/jas/skz258.611.

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Abstract Trihexanoin is a short-chain triglyceride (SCT). Many studies have reported that SCTs play important roles in the maintenance of intestinal epithelial structure and function. The present work was to investigate the effects of trihexanoin on growth performance, carbohydrate and fat metabolism, as well as intestinal morphology and function in weaned piglets. Twenty weaned piglets (21 ± 2 d) were randomly allocated to one of two treatment groups: the control group (basal diet supplemented with 0.5% soya oil); the TH group (basal diet supplemented with 0.5% trihexanoin). Dietary trihexanoin supplementation significantly reduced diarrhea rate (P &lt; 0.05); increased the concentrations of LDL, HDL and total protein, decreased cholesterol concentrations (CHOL) and glutamyl transpeptidase (GGT) activity in plasma (P &lt; 0.05); increased villus height, surface area, and the ratio of villus height to crypt depth (P &lt; 0.05); altered the mRNA levels and abundances of proteins related to glycogen and fat metabolism (gene LIPE, LPL, PPARG, ACACA, FASN, SLC27A2, INSR, PCK1 and ASS1), mucosal barrier function (protein claudin-1, and occludin), antioxidant capacity (protein HSP70 and gene Nrf2, NOX2 and GSTO2) and water transport capacity (protein AQP3 and gene AQP8 and AQP10) (P &lt; 0.05); altered the gene abundance of intestinal bacteria (Enterobacteriaceae, Enterococcus, Clostridium, Lactobacillus and Bifidobacterium) (P &lt; 0.05). In conclusion, dietary supplementation of trihexanoin improved the intestinal function and health of weaned piglets by regulating nutrient metabolism, improving intestinal function of mucosal barrier, transport, absorption and antioxidant, and altering the community of microbiota.
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Shi, Yong, Lei Zhong, Yanli Liu, Junzhi Zhang, Zhao Lv, Yao Li, and Yi Hu. "Effects of Dietary Andrographolide Levels on Growth Performance, Antioxidant Capacity, Intestinal Immune Function and Microbioma of Rice Field Eel (Monopterus Albus)." Animals 10, no. 10 (September 25, 2020): 1744. http://dx.doi.org/10.3390/ani10101744.

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An eight-week feeding trial was conducted to investigate the effects of dietary andrographolide on the growth performance, antioxidant capacity in the liver, intestinal inflammatory response and microbiota of Monopterus albus. A total of 900 health fish (25.00 ± 0.15 g) were randomly divided into five groups: AD1 (the basal diet) as the control, and AD2, AD3, AD4 and AD5 groups, which were fed the basal diet supplemented with 75, 150, 225 and 300 mg/kg andrographolide, respectively. The results showed that compared with the control group, dietary andrographolide supplementation (1) significantly increased trypsin and lipase activities in the intestine, and increased the weight gain rate but not significantly; (2) significantly increased the levels of glutathione reductase (GR), glutathione (GSH) and glutathione peroxidase (GPx) and the content of in the liver; significantly decreased the contents of reactive oxygen species (ROS) and malondialdehyde (MDA); remarkably upregulated the Nrf2, SOD1, GSTK and GSTO mRNA levels in the liver; downregulated the Keap1 mRNA level; (3) significantly increased the villi length and goblet cell numbers in the intestine, remarkably upregulated the Occludin mRNA level in the intestine, downregulated the Claudin-15 mRNA level; (4) remarkably upregulated the IL-10, TGF-β1 and TGF-β3 mRNA levels in the intestine; downregulated the IL-12β and TLR-3 mRNA levels; (5) significantly decreased the richness and diversity of the intestinal microbioma, increased the percentages of Fusobacteria and Firmicutes and significantly decreased the percentages of Cyanobacteria and Proteobacteria. In conclusion, these results showed that dietary low-dose andrographolide (75 and 150 mg/kg) promoted growth and antioxidant capacity, regulated the intestinal microbioma, enhanced intestinal physical and immune barrier function in rice field eel.
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Corredor, Zuray, Miguel Inácio da Silva Filho, Lara Rodríguez-Ribera, Antonia Velázquez, Alba Hernández, Calogerina Catalano, Kari Hemminki, et al. "Genetic Variants Associated with Chronic Kidney Disease in a Spanish Population." Scientific Reports 10, no. 1 (January 10, 2020). http://dx.doi.org/10.1038/s41598-019-56695-2.

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AbstractChronic kidney disease (CKD) patients have many affected physiological pathways. Variations in the genes regulating these pathways might affect the incidence and predisposition to this disease. A total of 722 Spanish adults, including 548 patients and 174 controls, were genotyped to better understand the effects of genetic risk loci on the susceptibility to CKD. We analyzed 38 single nucleotide polymorphisms (SNPs) in candidate genes associated with the inflammatory response (interleukins IL-1A, IL-4, IL-6, IL-10, TNF-α, ICAM-1), fibrogenesis (TGFB1), homocysteine synthesis (MTHFR), DNA repair (OGG1, MUTYH, XRCC1, ERCC2, ERCC4), renin-angiotensin-aldosterone system (CYP11B2, AGT), phase-II metabolism (GSTP1, GSTO1, GSTO2), antioxidant capacity (SOD1, SOD2, CAT, GPX1, GPX3, GPX4), and some other genes previously reported to be associated with CKD (GLO1, SLC7A9, SHROOM3, UMOD, VEGFA, MGP, KL). The results showed associations of GPX1, GSTO1, GSTO2, UMOD, and MGP with CKD. Additionally, associations with CKD related pathologies, such as hypertension (GPX4, CYP11B2, ERCC4), cardiovascular disease, diabetes and cancer predisposition (ERCC2) were also observed. Different genes showed association with biochemical parameters characteristic for CKD, such as creatinine (GPX1, GSTO1, GSTO2, KL, MGP), glomerular filtration rate (GPX1, GSTO1, KL, ICAM-1, MGP), hemoglobin (ERCC2, SHROOM3), resistance index erythropoietin (SOD2, VEGFA, MTHFR, KL), albumin (SOD1, GSTO2, ERCC2, SOD2), phosphorus (IL-4, ERCC4 SOD1, GPX4, GPX1), parathyroid hormone (IL-1A, IL-6, SHROOM3, UMOD, ICAM-1), C-reactive protein (SOD2, TGFB1,GSTP1, XRCC1), and ferritin (SOD2, GSTP1, SLC7A9, GPX4). To our knowledge, this is the second comprehensive study carried out in Spanish patients linking genetic polymorphisms and CKD.
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Piaggi, Simona, Evelina Lorenzini, Federico Pratesi, Paola Migliorini, Alfonso Pompella, Fabrizio Bruschi, and Alessandro Corti. "Anti-glutathione S-transferase omega 1-1 (GSTO1-1) antibodies are increased during acute and chronic inflammation in humans." Clinical and Experimental Immunology, June 22, 2022. http://dx.doi.org/10.1093/cei/uxac060.

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Abstract Glutathione S-transferase omega-1 (GSTO1-1) is a cytosolic enzyme involved in the modulation of critical inflammatory pathways as well as in cancer progression. Auto-antibodies against GSTO1-1 were detected in serum of patients with esophageal squamous cell carcinoma and were proposed as potential biomarkers in the early detection of the disease. Our data demonstrate that anti-GSTO1-1 antibodies can be found also in different types of inflammatory diseases, such as autoimmune rheumatoid arthritis and infectious SARS-CoV-2 and trichinellosis. Our data strongly suggest that anti-GSTO1-1 antibodies could represent a marker of tissue damage/inflammation rather than a type of specific tumor-associated biomarker only.
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Hancock, John T., David Veal, Tim J. Craig, and Ros C. Rouse. "Do SNPs in Glutathione S-Transferase-Omega Allow Predictions of the Susceptibility of Vertebrates to SARS-CoV-2?" Reactive Oxygen Species 12 (June 8, 2022). http://dx.doi.org/10.20455/ros.2022.c805.

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Infection with the SARS-Cov-2 virus causes COVID-19 in humans and is the cause of the pandemic around the world in 2020 and on. However, some animals have been found to be susceptible to the virus too. This has included the non-human primates, dogs, cats, and mustelids. Mink, and very recently hamsters and deer, have been shown to be able to contract the virus and pass it back to humans. However, which animals are susceptible to the virus has been very hard to predict. Many groups have looked at the sequence homology of the angiotensin converting enzyme 2 (ACE2), a receptor for the SARS-Cov-2 virus, across species, but this has had limited success. Similar work on other proteins such as transmembrane serine protease 2 (TMPRSS2), neuropilin-1, and furin have also been unfruitful. Recently, it has been suggested that single nucleotide polymorphisms (SNPs) in the glutathione S-transferase-omega (GSTO) genes of humans could alter viral susceptibility. Therefore, here, the presence of related sequences in vertebrates has been investigated. The SNPs in the GST-omega-1 (GSTO1) gene reported to increase COVID-19 in humans do not appear in the vertebrate species. However, the GST-omega-2 (GSTO2) SNP is represented in several vertebrate species known to have contracted the SAR-CoV-2 virus. Of course, animals may contain unknown SNPs at disruptive points in these genes too. In summary, GSTO1 genes are unlikely, at least at the moment, to be of value in predicting the susceptibility of an animal to the SARS-CoV-2 virus or disease progression, but a further study of the GST-omega-2 genes would be worthwhile. Therefore, more work on SARS-CoV-2 infections on vertebrates is recommended. (First Online: June 8, 2022)
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35

Ma, Dan, Ruiqing Yang, Yunlong Chen, Zhengyi Huang, Yuxin Shen, Chengqi He, and Lixing Zhao. "Identification of noninvasive diagnostic biomarkers for ectopic pregnancy using data-independent acquisition (DIA)proteomics: a pilot study." Scientific Reports 12, no. 1 (November 21, 2022). http://dx.doi.org/10.1038/s41598-022-23374-8.

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AbstractAt present, the diagnosis of ectopic pregnancy mainly depends on transvaginal ultrasound and β-hCG. However, these methods may delay diagnosis and treatment time. Therefore, we aimed to screen for serological molecular markers for the early diagnosis of ectopic pregnancy (EP).Using data-independent acquisition (DIA)proteomics, the differential proteins in serum were selected between the intrauterine pregnancy (IP) and EP groups. Then, the expression levels of these differential proteins were measured by enzyme-linked immunosorbent assay. The diagnostic value of the serum biomarkers was evaluated by receiver operating characteristic curve analysis.GSTO1, ECM-1 and β-hCG showed significant differences between the EP and IP groups (P < 0.05). The combination of GSTO1/ECM-1/β-hCG had an area under the curve of 0.93 (95% CI 0.88–0.99), a sensitivity of 88.89% (95% CI 73.94–96.89) and a specificity of 86.11% (95% CI 70.50–95.33) with a likelihood ratio of 6.40.The combination of GSTO1/ECM-1/β-hCG may be developed into a possible approach for the early diagnosis of EP.
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Walters, Elizabeth Anne, Gabriella Hubbard, Paula Sáez Ráez, Thomas Schmieta, and James William Walters. "Transcriptome Response of genes mitigating Reactive Oxygen Species in Zebrafish (Danio rerio) Larvae during dietary lipid absorption." Proceedings of the West Virginia Academy of Science 93, no. 1 (April 1, 2021). http://dx.doi.org/10.55632/pwvas.v93i1.455.

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The Mediterranean diet is associated with higher levels of polyunsaturated fatty acids (PUFAs) as well as lower incidences of cardiac disease and colon cancer (1). Due the presence of multiple double bonds, PUFAs such as alpha-linoleic acid (ALA), are thought to generate fewer harmful metabolites, specifically the Reactive Oxidative Species (ROS). Conversely, monounsaturated fatty acids (MUFAs) may lead to relatively increased ROS. In this study we compare two high-fat diets: the MUFA diet containing oleic acid (1 mM C18:1 plus 1 mM cholesterol) and the PUFA diet containing ALA (1 mM C18:3 plus 1 mM cholesterol). We compared gene expression from the dissected intestines of six days post-fertilization zebrafish (Danio rerio) larvae after feeding the PUFA and MUFA diets for 3 hours. We measured ROS response indirectly via the increased expression of ROS response genes (GSTO1, GSR, and cyp1a). Our preliminary results show upregulation of the ROS response genes GSTO1, GSR, and cyp1a in the presence of MUFA and await further replicates to become significant. . These data support the hypothesis that dietary fatty acid saturation plays a role in oxidative stress within enterocytes. In the future we plan to measure ROS responsiveness directly via respirometry. This work was supported by NIH Grant P20GM103434 to the West Virginia IDeA Network for Biomedical Research Excellence and NIH Grant P20GM103434 awarded to Bluefield State College and the McNair Scholars Program.
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Menon, Deepthi, Ashlee Innes, Aaron J. Oakley, Jane E. Dahlstrom, Lora M. Jensen, Anne Brüstle, Padmaja Tummala, et al. "GSTO1-1 plays a pro-inflammatory role in models of inflammation, colitis and obesity." Scientific Reports 7, no. 1 (December 2017). http://dx.doi.org/10.1038/s41598-017-17861-6.

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38

Zhu, Ying, Mirko Scheibinger, Daniel Christian Ellwanger, Jocelyn F. Krey, Dongseok Choi, Ryan T. Kelly, Stefan Heller, and Peter G. Barr-Gillespie. "Single-cell proteomics reveals changes in expression during hair-cell development." eLife 8 (November 4, 2019). http://dx.doi.org/10.7554/elife.50777.

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Hearing and balance rely on small sensory hair cells that reside in the inner ear. To explore dynamic changes in the abundant proteins present in differentiating hair cells, we used nanoliter-scale shotgun mass spectrometry of single cells, each ~1 picoliter, from utricles of embryonic day 15 chickens. We identified unique constellations of proteins or protein groups from presumptive hair cells and from progenitor cells. The single-cell proteomes enabled the de novo reconstruction of a developmental trajectory using protein expression levels, revealing proteins that greatly increased in expression during differentiation of hair cells (e.g., OCM, CRABP1, GPX2, AK1, GSTO1) and those that decreased during differentiation (e.g., TMSB4X, AGR3). Complementary single-cell transcriptome profiling showed corresponding changes in mRNA during maturation of hair cells. Single-cell proteomics data thus can be mined to reveal features of cellular development that may be missed with transcriptomics.
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Yu, Jie, Jie Fu, Xiaoyun Zhang, Xiaodong Cui, and Min Cheng. "The Integration of Metabolomic and Proteomic Analyses Revealed Alterations in Inflammatory-Related Protein Metabolites in Endothelial Progenitor Cells Subjected to Oscillatory Shear Stress." Frontiers in Physiology 13 (February 16, 2022). http://dx.doi.org/10.3389/fphys.2022.825966.

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BackgroundEndothelial progenitor cells (EPCs) play essential roles in vascular repair. Our previous study suggests OSS would lead EPCs transdifferention into the mesenchymal cell that aggravates pathological vascular remodeling. The primary purpose of this study was to apply OSS in vitro in EPCs and then explore proteins, metabolites, and the protein-metabolite network of EPCs.MethodsEndothelial progenitor cells were kept in static or treated with OSS. For OSS treatment, the Flexcell STR-4000 parallel plate flow system was used to simulate OSS for 12 h. Subsequently, an untargeted metabolomic LC/MS analysis and a TMT-labeled quantitative proteomic analysis were performed.ResultsA total of 4,699 differentially expressed proteins (DEPs) were identified, among which 73 differentially expressed proteins were potentially meaningful (P &lt; 0.05), with 66 upregulated and 7 downregulated expressions. There were 5,664 differential metabolites (DEMs), of which 401 DEMs with biologically potential marker significance (VIP &gt; 1, P &lt; 0.05), of which 137 were upregulated and 264 were downregulated. The Prison correlation analysis of DEPs and DEMs was performed, and the combined DEPs–DEMs pathway analyses of the KGLM database show 39 pathways. Among the DEPs, including the Phosphoserine phosphatase (PSPH), Prostaglandin E synthase 3 (PTGES3), Glutamate–cysteine ligase regulatory subunit (GCLM), Transaldolase (TALDO1), Isocitrate dehydrogenase 1 (IDH1) and Glutathione S-transferase omega-1 (GSTO1), which are significantly enriched in the citric acid cycle (TCA cycle) and fatty acid metabolic pathways, promoting glycolysis and upregulation of fatty acid synthesis. Moreover, we screened the 6 DEPs with the highest correlation with DEMs for predicting the onset of early AS and performed qPCR to validate them.ConclusionThe comprehensive analysis reveals the following main changes in EPCs after the OSS treatment: dysregulation of glutamate and glycine metabolism and their transport/catabolic related proteins. Disorders of fatty acid and glycerophospholipid metabolism accompanied by alterations in the corresponding metabolic enzymes. Elevated expression of glucose metabolism.
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