Journal articles on the topic 'GRNA'
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Hardiyani, Wulan Arum, Ali Wafa, Wahyu Indra Duwi Fanata, and Hardian Susilo Addy. "Design and construction of single guide RNA for CRISPR/Cas9 system based on the xa13 resistance gene in some varieties of rice (Oryza sativa)." Jurnal Hama dan Penyakit Tumbuhan Tropika 23, no. 1 (January 18, 2023): 47–55. http://dx.doi.org/10.23960/jhptt.12347-55.
Full textRead, L. K., H. U. Göringer, and K. Stuart. "Assembly of mitochondrial ribonucleoprotein complexes involves specific guide RNA (gRNA)-binding proteins and gRNA domains but does not require preedited mRNA." Molecular and Cellular Biology 14, no. 4 (April 1994): 2629–39. http://dx.doi.org/10.1128/mcb.14.4.2629-2639.1994.
Full textRead, L. K., H. U. Göringer, and K. Stuart. "Assembly of mitochondrial ribonucleoprotein complexes involves specific guide RNA (gRNA)-binding proteins and gRNA domains but does not require preedited mRNA." Molecular and Cellular Biology 14, no. 4 (April 1994): 2629–39. http://dx.doi.org/10.1128/mcb.14.4.2629.
Full textCruz-Reyes, Jorge, Alevtina Zhelonkina, Laura Rusche, and Barbara Sollner-Webb. "Trypanosome RNA Editing: Simple Guide RNA Features Enhance U Deletion 100-Fold." Molecular and Cellular Biology 21, no. 3 (February 1, 2001): 884–92. http://dx.doi.org/10.1128/mcb.21.3.884-892.2001.
Full textCollins, Scott P., William Rostain, Chunyu Liao, and Chase L. Beisel. "Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR–Cas12a gRNA switch." Nucleic Acids Research 49, no. 5 (February 22, 2021): 2985–99. http://dx.doi.org/10.1093/nar/gkab100.
Full textMoniruzzaman, M., Yun Zhong, Zhifeng Huang, and Guangyan Zhong. "Having a Same Type IIS Enzyme’s Restriction Site on Guide RNA Sequence Does Not Affect Golden Gate (GG) Cloning and Subsequent CRISPR/Cas Mutagenesis." International Journal of Molecular Sciences 23, no. 9 (April 28, 2022): 4889. http://dx.doi.org/10.3390/ijms23094889.
Full textForeman, Hui-Chen Chang, Varvara Kirillov, Gabrielle Paniccia, Demetra Catalano, Trevor Andrunik, Swati Gupta, Laurie T. Krug, and Yue Zhang. "RNA-guided gene editing of the murine gammaherpesvirus 68 genome reduces infectious virus production." PLOS ONE 16, no. 6 (June 4, 2021): e0252313. http://dx.doi.org/10.1371/journal.pone.0252313.
Full textClement, Sandra L., Melissa K. Mingler, and Donna J. Koslowsky. "An Intragenic Guide RNA Location Suggests a Complex Mechanism for Mitochondrial Gene Expression in Trypanosoma brucei." Eukaryotic Cell 3, no. 4 (August 2004): 862–69. http://dx.doi.org/10.1128/ec.3.4.862-869.2004.
Full textGoulah, Christopher C., and Laurie K. Read. "Differential Effects of Arginine Methylation on RBP16 mRNA Binding, Guide RNA (gRNA) Binding, and gRNA-containing Ribonucleoprotein Complex (gRNP) Formation." Journal of Biological Chemistry 282, no. 10 (January 17, 2007): 7181–90. http://dx.doi.org/10.1074/jbc.m609485200.
Full textTylec, Brianna L., Rachel M. Simpson, Laura E. Kirby, Runpu Chen, Yijun Sun, Donna J. Koslowsky, and Laurie K. Read. "Intrinsic and regulated properties of minimally edited trypanosome mRNAs." Nucleic Acids Research 47, no. 7 (January 30, 2019): 3640–57. http://dx.doi.org/10.1093/nar/gkz012.
Full textVichera, G., D. Viale, R. Olivera, V. Arnold, A. Grundnig, J. Baston, S. Miriuka, and L. Moro. "20 Generation of myostatin knockout horse embryos using clustered regularly interspaced short palindromic repeats/CRISPR-associated gene 9 and somatic cell nuclear transfer." Reproduction, Fertility and Development 31, no. 1 (2019): 136. http://dx.doi.org/10.1071/rdv31n1ab20.
Full textMekler, Vladimir, Konstantin Kuznedelov, and Konstantin Severinov. "Quantification of the affinities of CRISPR–Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences." Journal of Biological Chemistry 295, no. 19 (April 1, 2020): 6509–17. http://dx.doi.org/10.1074/jbc.ra119.012239.
Full textEspin Palazon, Raquel, Xiaoyi Cheng, Clyde A. Campbell, Liangdao Li, Bettina Schmid, and David Traver. "Zebra "Fishing" the Role of Granulin in Hematopoiesis." Blood 134, Supplement_1 (November 13, 2019): 1194. http://dx.doi.org/10.1182/blood-2019-130781.
Full textLapinaite, Audrone, Jennifer A. Doudna, and Jamie H. D. Cate. "Programmable RNA recognition using a CRISPR-associated Argonaute." Proceedings of the National Academy of Sciences 115, no. 13 (March 12, 2018): 3368–73. http://dx.doi.org/10.1073/pnas.1717725115.
Full textIgo, Robert P., Sobomabo D. Lawson, and Kenneth Stuart. "RNA Sequence and Base Pairing Effects on Insertion Editing in Trypanosoma brucei." Molecular and Cellular Biology 22, no. 5 (March 1, 2002): 1567–76. http://dx.doi.org/10.1128/mcb.22.5.1567-1576.2002.
Full textKirillov, Bogdan, Ekaterina Savitskaya, Maxim Panov, Aleksey Y. Ogurtsov, Svetlana A. Shabalina, Eugene V. Koonin, and Konstantin V. Severinov. "Uncertainty-aware and interpretable evaluation of Cas9–gRNA and Cas12a–gRNA specificity for fully matched and partially mismatched targets with Deep Kernel Learning." Nucleic Acids Research 50, no. 2 (November 17, 2021): e11-e11. http://dx.doi.org/10.1093/nar/gkab1065.
Full textBringaud, F., R. Stripecke, G. C. Frech, S. Freedland, C. Turck, E. M. Byrne, and L. Simpson. "Mitochondrial glutamate dehydrogenase from Leishmania tarentolae is a guide RNA-binding protein." Molecular and Cellular Biology 17, no. 7 (July 1997): 3915–23. http://dx.doi.org/10.1128/mcb.17.7.3915.
Full textJung, Soo Bin, Chae young Lee, Kwang-Ho Lee, Kyu Heo, and Si Ho Choi. "A cleavage-based surrogate reporter for the evaluation of CRISPR–Cas9 cleavage efficiency." Nucleic Acids Research 49, no. 15 (June 4, 2021): e85-e85. http://dx.doi.org/10.1093/nar/gkab467.
Full textDarcis, Gilles, Caroline Binda, Bep Klaver, Elena Herrera-Carrillo, Ben Berkhout, and Atze Das. "The Impact of HIV-1 Genetic Diversity on CRISPR-Cas9 Antiviral Activity and Viral Escape." Viruses 11, no. 3 (March 13, 2019): 255. http://dx.doi.org/10.3390/v11030255.
Full textEasmin, Farhana, Naim Hassan, Yu Sasano, Keisuke Ekino, Hisataka Taguchi, and Satoshi Harashima. "gRNA-transient expression system for simplified gRNA delivery in CRISPR/Cas9 genome editing." Journal of Bioscience and Bioengineering 128, no. 3 (September 2019): 373–78. http://dx.doi.org/10.1016/j.jbiosc.2019.02.009.
Full textYuan, Guoliang, Stanton Martin, Md Mahmudul Hassan, Gerald A. Tuskan, and Xiaohan Yang. "PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies." Cells 11, no. 16 (August 9, 2022): 2467. http://dx.doi.org/10.3390/cells11162467.
Full textKoreman, Gabriel T., Yineng Xu, Qinan Hu, Zijing Zhang, Sarah E. Allen, Mariana F. Wolfner, Bei Wang, and Chun Han. "Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis inDrosophila." Proceedings of the National Academy of Sciences 118, no. 14 (March 29, 2021): e2014255118. http://dx.doi.org/10.1073/pnas.2014255118.
Full textIgo, Robert P., Setareh S. Palazzo, Moffett L. K. Burgess, Aswini K. Panigrahi, and Kenneth Stuart. "Uridylate Addition and RNA Ligation Contribute to the Specificity of Kinetoplastid Insertion RNA Editing." Molecular and Cellular Biology 20, no. 22 (November 15, 2000): 8447–57. http://dx.doi.org/10.1128/mcb.20.22.8447-8457.2000.
Full textIgo, Robert P., David S. Weston, Nancy Lewis Ernst, Aswini K. Panigrahi, Reza Salavati, and Kenneth Stuart. "Role of Uridylate-Specific Exoribonuclease Activity in Trypanosoma brucei RNA Editing." Eukaryotic Cell 1, no. 1 (February 2002): 112–18. http://dx.doi.org/10.1128/ec.1.1.112-118.2002.
Full textPrajapati, Archana. "Towards the diagnosis of dengue virus and its serotypes using designed CRISPR/Cas13 gRNAs." Bioinformation 18, no. 8 (August 31, 2022): 661–68. http://dx.doi.org/10.6026/97320630018661.
Full textKhanal, Sushant, Dechao Cao, Jinyu Zhang, Yi Zhang, Madison Schank, Xindi Dang, Lam Ngoc Thao Nguyen, et al. "Synthetic gRNA/Cas9 Ribonucleoprotein Inhibits HIV Reactivation and Replication." Viruses 14, no. 9 (August 28, 2022): 1902. http://dx.doi.org/10.3390/v14091902.
Full textZimmer, Sara L., Sarah M. McEvoy, Jun Li, Jun Qu, and Laurie K. Read. "A Novel Member of the RNase D Exoribonuclease Family Functions in Mitochondrial Guide RNA Metabolism in Trypanosoma brucei." Journal of Biological Chemistry 286, no. 12 (January 20, 2011): 10329–40. http://dx.doi.org/10.1074/jbc.m110.152439.
Full textChamper, Samuel E., Suh Yeon Oh, Chen Liu, Zhaoxin Wen, Andrew G. Clark, Philipp W. Messer, and Jackson Champer. "Computational and experimental performance of CRISPR homing gene drive strategies with multiplexed gRNAs." Science Advances 6, no. 10 (March 2020): eaaz0525. http://dx.doi.org/10.1126/sciadv.aaz0525.
Full textXie, Kabin, Bastian Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system." Proceedings of the National Academy of Sciences 112, no. 11 (March 2, 2015): 3570–75. http://dx.doi.org/10.1073/pnas.1420294112.
Full textCooper, Sinclair, Elizabeth S. Wadsworth, Torsten Ochsenreiter, Alasdair Ivens, Nicholas J. Savill, and Achim Schnaufer. "Assembly and annotation of the mitochondrial minicircle genome of a differentiation-competent strain of Trypanosoma brucei." Nucleic Acids Research 47, no. 21 (October 30, 2019): 11304–25. http://dx.doi.org/10.1093/nar/gkz928.
Full textMcManus, Michael T., Brian K. Adler, Victoria W. Pollard, and Stephen L. Hajduk. "Trypanosoma brucei Guide RNA Poly(U) Tail Formation Is Stabilized by Cognate mRNA." Molecular and Cellular Biology 20, no. 3 (February 1, 2000): 883–91. http://dx.doi.org/10.1128/mcb.20.3.883-891.2000.
Full textChameettachal, Akhil, Valérie Vivet-Boudou, Fathima Nuzra Nagoor Pitchai, Vineeta N. Pillai, Lizna Mohamed Ali, Anjana Krishnan, Serena Bernacchi, Farah Mustafa, Roland Marquet, and Tahir A. Rizvi. "A purine loop and the primer binding site are critical for the selective encapsidation of mouse mammary tumor virus genomic RNA by Pr77Gag." Nucleic Acids Research 49, no. 8 (April 9, 2021): 4668–88. http://dx.doi.org/10.1093/nar/gkab223.
Full textXu, Jianyong, Wenlei Li, MD Munnaf Hossen, Yuning Jia, Lingyun Li, and Zhong Huang. "Optimized Plasmid Construction Strategy for Cas9." Cellular Physiology and Biochemistry 48, no. 1 (2018): 131–37. http://dx.doi.org/10.1159/000491669.
Full textSimon, Ian D., Nico van Rooijen, and John K. Rose. "Vesicular Stomatitis Virus Genomic RNA Persists In Vivo in the Absence of Viral Replication." Journal of Virology 84, no. 7 (December 23, 2009): 3280–86. http://dx.doi.org/10.1128/jvi.02052-09.
Full textKe, Yuqing, Behafarid Ghalandari, Shiyi Huang, Sijie Li, Chengjie Huang, Xiao Zhi, Daxiang Cui, and Xianting Ding. "2′-O-Methyl modified guide RNA promotes the single nucleotide polymorphism (SNP) discrimination ability of CRISPR–Cas12a systems." Chemical Science 13, no. 7 (2022): 2050–61. http://dx.doi.org/10.1039/d1sc06832f.
Full textJameel, Mohd Rizwan. "From design to validation of CRISPR/gRNA primers towards genome editing." Bioinformation 18, no. 5 (May 31, 2022): 471–77. http://dx.doi.org/10.6026/97320630018471.
Full textRoberson, Elisha D. "Identification of high-efficiency 3′GG gRNA motifs in indexed FASTA files with ngg2." PeerJ Computer Science 1 (November 18, 2015): e33. http://dx.doi.org/10.7717/peerj-cs.33.
Full textKawashima, Nozomu, Yusuke Okuno, Yuko Sekiya, Xinan Wang, Yinyan Xu, Atsushi Narita, Sayoko Doisaki, et al. "Generation of Cell Lines Harboring SETBP1 Mutations By the Crispr/Cas9 System." Blood 124, no. 21 (December 6, 2014): 4622. http://dx.doi.org/10.1182/blood.v124.21.4622.4622.
Full textMullally, Grace, Kara van Aelst, Mohsin M. Naqvi, Fiona M. Diffin, Tautvydas Karvelis, Giedrius Gasiunas, Virginijus Siksnys, and Mark D. Szczelkun. "5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage." Nucleic Acids Research 48, no. 12 (June 4, 2020): 6811–23. http://dx.doi.org/10.1093/nar/gkaa477.
Full textBhagwat, Aditya M., Johannes Graumann, Rene Wiegandt, Mette Bentsen, Jordan Welker, Carsten Kuenne, Jens Preussner, Thomas Braun, and Mario Looso. "multicrispr: gRNA design for prime editing and parallel targeting of thousands of targets." Life Science Alliance 3, no. 11 (September 9, 2020): e202000757. http://dx.doi.org/10.26508/lsa.202000757.
Full textBinda, Caroline S., Bep Klaver, Ben Berkhout, and Atze T. Das. "CRISPR-Cas9 Dual-gRNA Attack Causes Mutation, Excision and Inversion of the HIV-1 Proviral DNA." Viruses 12, no. 3 (March 18, 2020): 330. http://dx.doi.org/10.3390/v12030330.
Full textMenees, Thomas M. "RNA Lariat Debranching Enzyme as a Retroviral and Long-Terminal-Repeat Retrotransposon Host Factor." Annual Review of Virology 7, no. 1 (September 29, 2020): 189–202. http://dx.doi.org/10.1146/annurev-virology-012720-094902.
Full textBreunig, Christopher T., Tamara Durovic, Andrea M. Neuner, Valentin Baumann, Maximilian F. Wiesbeck, Anna Köferle, Magdalena Götz, Jovica Ninkovic, and Stefan H. Stricker. "One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR)." PLOS ONE 13, no. 4 (April 27, 2018): e0196015. http://dx.doi.org/10.1371/journal.pone.0196015.
Full textArakawa, Hiroshi. "A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism." Science Advances 2, no. 8 (August 2016): e1600699. http://dx.doi.org/10.1126/sciadv.1600699.
Full textRice, Breanna L., Timothy L. Lochmann, and Leslie J. Parent. "RNA-Binding Domains of Heterologous Viral Proteins Substituted for Basic Residues in the RSV Gag NC Domain Restore Specific Packaging of Genomic RNA." Viruses 12, no. 4 (March 27, 2020): 370. http://dx.doi.org/10.3390/v12040370.
Full textGarbitt-Hirst, Rachel, Scott P. Kenney, and Leslie J. Parent. "Genetic Evidence for a Connection between Rous Sarcoma Virus Gag Nuclear Trafficking and Genomic RNA Packaging." Journal of Virology 83, no. 13 (April 15, 2009): 6790–97. http://dx.doi.org/10.1128/jvi.00101-09.
Full textSeraj, Zeba I., and Sabrina Hque. "Remodelling of a bacterial immune system as the simple gene editing tool, Crispr-Cas, for food security and human health." Journal of Bangladesh Academy of Sciences 45, no. 2 (January 27, 2022): 131–45. http://dx.doi.org/10.3329/jbas.v45i2.57208.
Full textMayes, Cathryn Michelle, and Joshua Santarpia. "Evaluating the Impact of gRNA SNPs in CasRx Activity for Reducing Viral RNA in HCoV-OC43." Cells 11, no. 12 (June 7, 2022): 1859. http://dx.doi.org/10.3390/cells11121859.
Full textCifuentes-Rojas, Catherine, Paula Pavia, Alfredo Hernandez, Daniel Osterwisch, Concepcion Puerta, and Jorge Cruz-Reyes. "Substrate Determinants for RNA Editing and Editing Complex Interactions at a Site for Full-round U Insertion." Journal of Biological Chemistry 282, no. 7 (December 7, 2006): 4265–76. http://dx.doi.org/10.1074/jbc.m605554200.
Full textSarni, Samantha, Banhi Biswas, Shuohui Liu, Erik D. Olson, Jonathan P. Kitzrow, Alan Rein, Vicki H. Wysocki, and Karin Musier-Forsyth. "HIV-1 Gag protein with or without p6 specifically dimerizes on the viral RNA packaging signal." Journal of Biological Chemistry 295, no. 42 (August 13, 2020): 14391–401. http://dx.doi.org/10.1074/jbc.ra120.014835.
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