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Journal articles on the topic 'Grapevines'
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1
Kiss, Tomáš, Mária Kocanová, Aleš Vavřiník, Dorota Tekielska, Jakub Pečenka, Eliška Hakalová, Milan Špetík, et al. "Incidence of GLMD-Like Symptoms on Grapevines Naturally Infected by Grapevine Pinot gris virus, Boron Content and Gene Expression Analysis of Boron Metabolism Genes." Agronomy 11, no. 6 (May 21, 2021): 1020. http://dx.doi.org/10.3390/agronomy11061020.
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Grapevine Pinot gris virus (GPGV) is considered to be a causal agent of Grapevine Leaf Mottling and Deformation (GLMD) disease that has been reported worldwide through the grapevine-growing regions. Seven grapevines that were collected from a vineyard in the Czech Republic were tested for the presence of GPGV in leaf and phloem tissues. Each of the seven grapevines was infected by GPGV, from which sic symptoms were mostly shown without a typical mottling. The phylogeny based on RNA-dependent RNA polymerase and movement/coat protein sequences indicated the same origin of the GPGV isolates. The GPGV titer was the highest in the grapevines with the highest GLMD-like symptoms; however, some of the grapevines with milder GLMD-like symptoms had a lower GPGV titer than the asymptomatic grapevine. Soil analysis showed uneven boron content in the direct vicinity of the grapevines, while the boron content in the grapevines was more, even showing no boron deficiency. The quantitative analysis of selected gene expressions associated with boron efflux and transport only partially explained the boron content in the soil and grapevines and only in the grapevines growing in soils with the highest or lowest boron contents. The VvBor2 and VvNIP5 genes had a higher expression and VvNIP6 had a lower expression in the grapevine growing in the soil with the lowest boron content, while a low expression of VvBor1 and VvBor2 was observed in the grapevine that was grown in the soil with the highest boron content.
2
Cholet, Céline, Émilie Bruez, Pascal Lecomte, Audrey Barsacq, Tommaso Martignon, Massimo Giudici, Marco Simonit, Denis Dubourdieu, and Laurence Gény. "Plant resilience and physiological modifications induced by curettage of Esca-diseased grapevines." OENO One 55, no. 1 (February 12, 2021): 153–69. http://dx.doi.org/10.20870/oeno-one.2021.55.1.4478.
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The re-emergence of Grapevine Trunk Diseases (GTDs), mainly Esca, has been observed in most of the world’s vineyards during the last two decades. Development of necrosis in grapevine wood, especially white-rot, is typically associated with Esca-diseased plants. One of the different methods being used in attempts to eradicate GTDs is curettage. This old cultural practice, which consists in surgically removing the necrotic wood, specifically white-rot, retaining only the non-necrotic tissue of Esca-diseased grapevine, is used in some European vineyards (Spain, France, Italy, Portugal), and is being increasingly reintroduced since 10 years ago in France. We, therefore, wanted to study the effect of curettage on vigour, fertility and berry quality, and year after year plant recovery. Our study synthetizes a 3-year experiment on Esca-diseased cv. Sauvignon blanc grapevines curetted in a commercial plot in the Bordeaux region. Asymptomatic control grapevines were compared to Esca-diseased grapevines without curettage (with typical foliar symptoms), and with curetted Esca-diseased grapevines (without foliar symptoms). Even if the curetted grapevines recovered lower vigour and fertility than the control plants, their grape berry quality was comparable, unlike for Esca-diseased grapevines. This cultural practice proved particularly effective in helping Esca-symptomatic grapevines to recover asymptomatic after treatment. Over time, curettage induces the resilience of grapevines, allowing them to recuperate their full physiological functioning, thereby compensating for Esca’s detrimental impact on berry quality.
3
Eichmeier, Aleš, Eliška Peňázová, Jana Čechová, and Akila Berraf-Tebbal. "Survey and Diversity of Grapevine Pinot gris virus in Algeria and Comprehensive High-Throughput Small RNA Sequencing Analysis of Two Isolates from Vitis vinifera cv. Sabel Revealing High Viral Diversity." Genes 11, no. 9 (September 22, 2020): 1110. http://dx.doi.org/10.3390/genes11091110.
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Grapevine Pinot gris virus (GPGV) is a putative causal agent of grapevine leaf mottling and deformation disease that has been reported worldwide throughout the grapevine-growing regions. Fifty-four grapevines collected from five Algerian grapevine-growing regions were tested for the presence of GPGV in phloem tissues. Eight of the tested grapevines were infected by GPGV. Viromes of two selected Vitis vinifera cv. Sabel grapevines infected by GPGV and showing virus-like symptoms were analyzed by small RNA sequencing. Phylogenetic analyses of the partial coding sequence (cds) of the RNA-dependent RNA polymerase (RdRp) domain showed that all Algerian GPGV isolates were grouped with some already-described asymptomatic isolates. This study provides the first survey of the occurrence of GPGV in Algeria. Moreover, Grapevine fleck virus, Grapevine rupestris stem pitting-associated virus, Grapevine virus B, Grapevine rupestris vein feathering virus, Hop stunt viroid and Grapevine yellow speckle viroid 1 were detected in Algeria for the first time.
4
Sosnowski, M. R., R. Lardner, T. J. Wicks, and E. S. Scott. "The Influence of Grapevine Cultivar and Isolate of Eutypa lata on Wood and Foliar Symptoms." Plant Disease 91, no. 8 (August 2007): 924–31. http://dx.doi.org/10.1094/pdis-91-8-0924.
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Grapevine cultivar (Vitis vinifera) and isolate of Eutypa lata influence wood and foliar symptoms of Eutypa dieback. Foliar symptoms of Eutypa dieback developed within 8 months of inoculating young grapevines (cvs. Grenache, Cabernet Sauvignon, and Merlot) in a shadehouse. Isolates of E. lata from various wine regions in southern Australia varied in their ability to colonize inoculated grapevines and induce wood and foliar symptoms. Grapevine cultivars varied for wood and foliar symptom expression but not for mycelial colonization. However, the severity of foliar symptoms was not related to the rate of spread of the fungus in the grapevine. Furthermore, the staining of wood typically attributed to E. lata did not reflect the presence of the fungus because the fungus was detected up to 80 mm beyond the stain. A field trial with mature grapevines revealed significant differences in the rate of spread of wood staining due to E. lata among eight cultivars, with up to 50 mm/year detected in Cabernet Sauvignon and Shiraz grapevines. In the shadehouse, the maximum growth rate of E. lata was recorded to be 115 mm/year for Grenache rootlings. Information from this study may help to optimize management strategies for maintaining productivity of grapevines with Eutypa dieback, thus reducing the economic impact of the disease.
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Zhang, Yu, Kashmir Singh, Ravneet Kaur, and Wenping Qiu. "Association of a Novel DNA Virus with the Grapevine Vein-Clearing and Vine Decline Syndrome." Phytopathology® 101, no. 9 (September 2011): 1081–90. http://dx.doi.org/10.1094/phyto-02-11-0034.
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A severe vein-clearing and vine decline syndrome has emerged on grapevines (Vitis vinifera) and hybrid grape cultivars in the Midwest region of the United States. The typical symptoms are translucent vein-clearing on young leaves, short internodes and decline of vine vigor. Known viral pathogens of grapevines were not closely associated with the syndrome. To obtain a comprehensive profile of viruses in a diseased grapevine, small RNAs were enriched and two cDNA libraries were constructed from a symptomatic grapevine and a symptomless grapevine, respectively. Deep sequencing of the two cDNA libraries showed that the most abundant viral small RNAs align with the genomes of viruses in the genus Badnavirus, the family Caulimoviridae. Amplification of the viral DNA by polymerase chain reaction allowed the assembly of the whole genome sequence of a grapevine DNA virus, which shared the highest homology with the Badnavirus sequences. This is the first report of a DNA virus in grapevines. The new DNA virus is closely associated with the vein-clearing symptom, and thus has been given a provisional name Grapevine vein clearing virus (GVCV). GVCV was detected in six grapevine cultivars showing vein-clearing and vine decline syndrome in Missouri, Illinois, and Indiana, suggesting its wide distribution in the Midwest region of the United States. Discovery of DNA viruses in grapevines merits further studies on their epidemics and economic impact on grape production worldwide.
6
Brannen, Phillip M., C. Michael Deom, O. J. Alabi, and Rayapati A. Naidu. "Prevalence of Viruses in Commercial Wine Grape Vineyards in Georgia." Plant Health Progress 19, no. 4 (January 1, 2018): 342–46. http://dx.doi.org/10.1094/php-07-18-0040-s.
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Virus diseases are major constraints to Vitis vinifera wine grape production worldwide. Grapevine leafroll-associated virus 3 (GLRaV-3) was first confirmed in Georgia in 2008. The negative impacts of GLRaV-3, such as decreased fruit yield and quality, were confirmed from samples taken in 2009 and 2010. In light of these findings, studies were initiated to determine the prevalence and types of grapevine viruses present in vineyards in Georgia. Five vineyard blocks were visited during August and September of 2011 and 2012. Leaf samples were collected from a total of 50 grapevines showing symptoms of and suspected as being infected by grapevine leafroll disease (GLD). Samples from individual grapevines were extracted and tested separately for 20 grapevine viruses listed in standard virus indexing programs. Reverse transcription polymerase chain reaction (RT-PCR) or PCR, depending on the nature of the virus genome, was used to detect these viruses with species-specific primers. The results showed the presence of GLRaV-1, -2, -3, and -4 (strains 4, 5, and 6), grapevine virus B, grapevine rupestris stem pitting-associated virus, and grapevine red blotch virus. Many vineyards had multiple viruses detected from individual grapevines showing typical GLD or GLD-like symptoms.
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Baumgartner, Kendra. "Root Collar Excavation for Postinfection Control of Armillaria Root Disease of Grapevine." Plant Disease 88, no. 11 (November 2004): 1235–40. http://dx.doi.org/10.1094/pdis.2004.88.11.1235.
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Root collar excavation for control of Armillaria root disease of grapevine was investigated in two California vineyards (vineyard K1 and vineyard N1) from 2002 to 2004. The hypothesis tested was that root collar excavation, when timed in early stages of root collar infection, may cause mycelial fans of the pathogen Armillaria mellea to recede from the root collar before severe disease results from vascular tissue decay. In vineyard N1, excavation significantly increased yield and cluster weight of symptomatic grapevines; symptomatic-excavated grapevines had the same high mean cluster weight as healthy grapevines, and there were no significant effects of excavation on yield or pruning weight of healthy grapevines. In vineyard K1, where excavated root collars frequently refilled with soil, excavation had no significant effects on yield or pruning weight of symptomatic grapevines, and significantly reduced pruning weight and shoot weight of healthy grapevines. Reexamination in March 2004 revealed that mycelial fans had receded from root collars of symptomatic-excavated grapevines, but remained on root collars of symptomatic-nonexcavated grapevines. Root collar excavation appears to be a promising cultural approach for control of Armillaria root disease, as long as excavated root collars are kept clear of soil.
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Guta, Ionela-Catalina, and Elena-Cocuta Buciumeanu. "Grapevine Pinot gris virus infecting grapevines in Romania - Short Communicaiton." Horticultural Science 48, No. 1 (March 31, 2021): 47–50. http://dx.doi.org/10.17221/65/2020-hortsci.
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Grapevine Pinot gris virus (GPGV) has been identified in many grape growing countries of the world since 2012. The aim of this work was to investigate the presence of GPGV on some accessions collected from a germplasm collection, in addition to the propagation material and clonal selection samples. During 2019–2020, a total of 199 samples have been analysed by a double antibody sandwich – enzyme-linked immunosorbent assay (DAS-ELISA) for the presence of GPGV, Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus-1+3 (GLRaV-1+3) and Grapevine fleck virus (GFkV). Among them, 107 samples (53.76%) showed a GPGV-infection, associated with or without symptoms on the leaves (deformations, chlorosis, mosaic, wrinkles) or stunting plants. The distribution of infected varieties showed a high rate of infection in old varieties (37.38%), followed by clones (32.71%), rootstocks (11.21%), clonal selections (9.35%) and new varieties (9.35%). The tests revealed the association of GPGV with GFkV (5 cases) and GLRaV-1+3 (2 cases). GPGV should be included in the rules of grapevine certification schemes for the production of virus-free mother plants.
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Ježić, Marin, Jasminka Karoglan Kontić, Darko Preiner, Edi Maletić, and Mirna Ćurković-Perica. "Grapevine yellows affecting the Croatian indigenous grapevine cultivar Grk." Acta Botanica Croatica 72, no. 2 (October 1, 2013): 287–94. http://dx.doi.org/10.2478/botcro-2013-0007.
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Abstract - The grapevine cultivar Grk, a close relative of Crljenak kaštelanski/Zinfandel, is grown exclusively in southern Croatia. Grapevine yellows-like symptoms were observed on vines in the vineyards in Lumbarda (southern Croatia) and in propagated grapevines near Zadar and Zagreb. The majority of the detected phytoplasma isolates belonged to the 16SrI group. However, RFLP pattern and R16F2n/R2 fragment sequence assigned one isolate to the 16SrIII group. Thus far, on cv. Grk, phytoplasmas belonging to three different groups have been detected: 16SrI, 16SrIII, and 16SrXII, which was confirmed previously. Aside from the 16SrI, 16SrV and 16SrXII phytoplasma groups previously found on grapevines in Croatia, the finding of 16SrIII group, which is not common on grapevines in Europe, adds to the diversity of phytoplasmas in a very small geographic region.
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Alcorta, Marisa, Matthew W. Fidelibus, Kerri L. Steenwerth, and Anil Shrestha. "Competitive Effects of Glyphosate-Resistant and Glyphosate-Susceptible Horseweed (Conyza canadensis) on Young Grapevines (Vitis vinifera)." Weed Science 59, no. 4 (December 2011): 489–94. http://dx.doi.org/10.1614/ws-d-10-00186.1.
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Horseweed is a common pest in vineyards of the San Joaquin Valley (SJV) of California. Interest in controlling this weed has increased with the recent discovery of a glyphosate-resistant (GR) biotype that has been observed to be more vigorous than a glyphosate-susceptible (GS) biotype in the SJV. However, the impact that either biotype may have on grapevine growth has not been assessed. Therefore, two glasshouse experiments were conducted to characterize the competitiveness of GR and GS horseweed biotypes from the SJV with young grapevines. ‘Syrah’ grapevines grafted to Freedom rootstocks were planted in 8-L plastic pots, alone, or with a single GR or GS horseweed. Additional GR and GS horseweeds were also planted separately in individual pots, and all plants were grown for 14 and 16 wk in 2006 and 2007, respectively. Grapevines grown with either biotype of the weed produced fewer leaves and amassed approximately 20% less dry mass (DM) than vines grown alone. The GR biotype reduced grapevine stem DM and length by 30%, but the GS biotype did not. The GR biotype accumulated more than twice the DM as the GS biotype, whether in competition with grapevine or not. Grapevines reduced the total leaf number of both horseweed biotypes by almost 50% and aboveground DM of GR and GS biotypes by 50 and 75%, respectively. These preliminary findings indicate that competition from horseweed can substantially reduce the growth of young grapevines and that the GR biotype may be more competitive than the GS biotype.
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West, E. R., E. J. Cother, C. C. Steel, and G. J. Ash. "The characterization and diversity of bacterial endophytes of grapevine." Canadian Journal of Microbiology 56, no. 3 (March 2010): 209–16. http://dx.doi.org/10.1139/w10-004.
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The diversity of culturable and nonculturable bacterial endophytes of grapevine ( Vitis vinifera L.) was examined using a combination of cultivation and molecular methods. Entire grapevines were sampled to characterize bacterial diversity from different locations throughout the vine. Gas chromatography of fatty acid methyl esters (FAMEs) was used to identify culturable isolates prior to subsequent further microbiological characterization, whilst denaturing gradient gel electrophoresis (DGGE) was used to profile the ribosomal DNA of the bacterial endophyte community extracted from grapevines. Gas chromatography of FAMEs identified 75% of culturable bacterial endophytes to genus level (similarity index >0.3). Many isolates were identified as Bacillus spp., Pseudomonas spp., and Curtobacterium spp. Additionally, actinomycetes are reported for the first time as endophytes of grapevines, with a number of isolates identified as Streptomyces spp. DGGE was successfully used to identify major bands present in samples and indicated a degree of homogeneity of bacterial endophyte community profiles within the grapevines sampled. The major bacterial bands were sequenced and used in identification. Comparison with bacterial markers produced from cultured bacterial endophytes suggested that bacteria in the DGGE profiles were not the species most commonly cultured. Additional research demonstrated similarities between epiphytic and endophytic populations and examined potential entry vectors. Endophyte entry was demonstrated in both field-grown and potted grapevines (‘Chardonnay’) using a rifampicin-resistant Bacillus cereus mutant. The possibility of grapevine epiphytes becoming endophytes, if the opportunity arises, was supported by comparison of gas chromatography of FAMEs from epiphytic and endophytic populations. This research adds grapevine bacterial endophyte communities to those that have been characterized by a multifaceted approach.
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Fan, Xudong, Zunping Zhang, Fang Ren, Guojun Hu, Chen Li, Baodong Zhang, and Yafeng Dong. "Development of a Full-Length Infectious cDNA Clone of the Grapevine Berry Inner Necrosis Virus." Plants 9, no. 10 (October 11, 2020): 1340. http://dx.doi.org/10.3390/plants9101340.
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Grapevine berry inner necrosis virus (GINV) belongs to the genus Trichovirus in the family Betaflexiviridae. The GINV isolate LN_BETA_RS was obtained from a “Beta” grapevine (Vitis riparia × Vitis labrusca) exhibiting chlorotic mottling and ring spot in Xingcheng, Liaoning Province, China. To verify the correlation between GINV and grapevine chlorotic mottling and ring spot disease, we constructed an infectious cDNA clone of GINV isolate LN_BETA_RS using the seamless assembly approach. Applied treatments of agroinfiltration infectious cDNA confirmed systemic GINV infection of the Nicotianaoccidentalis 37B by reverse transcription polymerase chain reaction (RT-PCR) and transmission electron microscopy, exhibiting chlorotic mottling symptoms on leaves. Infectious cDNA was also transmitted to new healthy N. occidentalis plants through rub-inoculation. Moreover, the cDNA clone was agroinfiltrated into “Beta” and “Thompson Seedless” grapevine plantlets, and the inoculated grapevines exhibited leaf chlorotic mottling and ringspot during the two years of observation. GINV-inoculated “Beta” grapevines had serious leaf chlorotic mottling and ringspot symptoms on the whole plant, while relatively few symptoms were observed on the leaves of agroinoculated “Thompson Seedless” grapevines in early spring and only weak ring spot gradually appeared later in the top young leaves. Our experiments fulfilled Koch’s postulates and revealed the causative role of GINV in grapevine chlorotic mottling and ring spot disease.
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Gkikas, Fedon-Ioannis, Alexandros Tako, Danai Gkizi, Christina Lagogianni, Emmanouil A. Markakis, and Sotirios E. Tjamos. "Paenibacillus alvei K165 and Fusarium oxysporum F2: Potential Biocontrol Agents against Phaeomoniella chlamydospora in Grapevines." Plants 10, no. 2 (January 22, 2021): 207. http://dx.doi.org/10.3390/plants10020207.
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In the last two decades grapevine trunk diseases (GTDs) have emerged as the most significant threat for grapevine sustainability worldwide. The tracheomycotic fungus Phaeomoniella chlamydospora (Pch) is the predominant GTD-associated species and cannot be controlled with available chemicals. In the present study, we evaluated the effectiveness of two microbial strains (Paenibacillus alvei K165 and Fusarium oxysporum F2) against Pch in grapevine. In vitro bioassays, performed in a growth culture medium simulating the xylem environment, indicated that F2 decreased Pch growth and sporulation, whereas K165 did not have any effect on Pch growth. In planta experiments revealed that root-drench and stem-puncture application of K165 and F2 reduced the endophytic relative DNA amount of Pch by 90% and 82%, respectively, compared to controls. However, wood discoloration, the typical symptom of Pch infection, was not reduced in the F2 treated grapevines. Nevertheless, the F2 treated grapevines harbored higher lignin levels compared to mocks, as it was also done by K165. Therefore, F2 and K165 have the potential to be used as biocontrol agents against Pch in grapevines.
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Carlucci, Antonia, Francesca Cibelli, Francesco Lops, and Maria Luisa Raimondo. "Characterization of Botryosphaeriaceae Species as Causal Agents of Trunk Diseases on Grapevines." Plant Disease 99, no. 12 (December 2015): 1678–88. http://dx.doi.org/10.1094/pdis-03-15-0286-re.
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Botryosphaeriaceae spp. have a cosmopolitan distribution and a wide range of plant hosts. Over the last 15 years, worldwide, 21 species of this family have been associated with grapevine trunk diseases that cause cankers and dieback on grapevines. Here, we surveyed vineyards of Vitis vinifera ‘Lambrusco’, ‘Sangiovese’, and ‘Montepulciano’ in three areas of the Foggia province (Cerignola, Foggia, and San Severo) in southern Italy. Wood samples from grapevines showing general decline, dieback, cankers, and wood and foliar discoloration yielded 344 fungal isolates identified as Botryosphaeriaceae spp. A phylogenetic study combining internal transcribed spacer and translation elongation factor 1-α sequences of 60 representative isolates identified nine botryosphaeriaceous species: Botryosphaeria dothidea, Diplodia corticola, D. mutila, D. seriata, Dothiorella iberica, Do. sarmentorum, Lasiodiplodia citricola, L. theobromae, and Neofusicoccum parvum. Pathogenicity tests confirmed that all nine species cause canker and dieback of grapevines. However, this is the first report of L. citricola as causal agent of wood cankers and dieback of grapevine. To date, including L. citricola, there are 25 botryosphaeriaceous species associated with V. vinifera worldwide, of which 12 have been reported for grapevines in Italy.
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Romanazzi, Gianfranco, Sergio Murolo, and Erica Feliziani. "Effects of an Innovative Strategy to Contain Grapevine Bois Noir: Field Treatment with Resistance Inducers." Phytopathology® 103, no. 8 (August 2013): 785–91. http://dx.doi.org/10.1094/phyto-01-13-0031-r.
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Grapevine Bois noir (BN) is a phytoplasma disease that is widespread in most viticultural regions of the world, and it can result in heavy reductions to yields and grape juice quality. At present, there is no effective strategy to reduce the incidence of BN-infected grapevines. However, phytoplasma-infected plants can recover through spontaneous or induced symptom remission. Five elicitors (chitosan, two glutathione-plus-oligosaccharine formulations, benzothiadiazole, and phosetyl-Al) were applied weekly to the canopy of BN-infected ‘Chardonnay’ grapevines from early May to late July. The best and most constant recovery inductions were obtained with benzothiadiazole and the two glutathione-plus-oligosaccharine formulations. The plants that recovered naturally or following the elicitors showed qualitative and quantitative parameters of production no different from healthy plants. In another vineyard, diseased plants showed reduced shoot length and production compared with healthy plants, and there were no negative effects on these parameters for grapevines sprayed with a glutathione-plus-oligosaccharine formulation. The application of resistance inducers promoted the recovery of BN-infected grapevines with no adverse effects on the plants. Therefore, grapevine can be used as a model species to test this innovative strategy to contain phytoplasma diseases.
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Štrukelj, Melita, Jaka RAZINGER, Barbara GRUBAR, Uroš ŽIBRAT, Irena MAVRIČ PLEŠKO, Dominik Vodnik, and Gregor UREK. "Fiziološki odziv žlahtne vinske trte Vitis vinifera L. na okužbo z zvijanjem listov vinske trte povezanih virusov (GLRaV-1 in GLRaV-1 + GLRaV-3)." Acta agriculturae Slovenica 107, no. 2 (October 26, 2016): 519. http://dx.doi.org/10.14720/aas.2016.107.2.22.
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Grapevine leafroll disease is one of the most severe viral diseases of grapevine caused by Grapevine leafroll-associated viruses (GLRaVs). Physiological processes were monitored on grapevines with single (GLRaV-1) and mixed (GLRaV-1 and GLRaV -3) viral infection under greenhouse conditions from June to September, in vegetation period 2014. In the mid of the season (July) negative effects of the virus infections on physiological processes were more severe in mixed than in single infection. The net-photosynthesis (Pn) of the leaves infected with GLRaV-1 and GLRaV-3 reached only a half of the Pn in GLRaV-1 infected grapevines. Similar reduction was found for stomatal conductance, transpiration and parameters related to photochemical efficiency (electron transport rate).
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Celik, H., B. Kose, and R. Cangi. "Determination of Fox grape genotypes (Vitis labrusca L.) grown in Northeastern Anatolia." Horticultural Science 35, No. 4 (December 15, 2008): 162–70. http://dx.doi.org/10.17221/655-hortsci.
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Northeastern part of Anatolia is a gene center of grapevine and a wide diversity of Fox grapes grow here. Viticulture and some grapevines in the Black Sea Region (in the north of Turkey) are on the verge of extinction; several types and varieties grown in this region have never been described and did not survive till present. For the past 10 years vineyards and grapevines in the Black Sea Region have been studied. In the present study, eighteen grapevines of foxy taste grown in the Artvin and Rize provinces were described. An ampelographic description of young shoot, mature shoot, young leaf, mature leaf, berry, bunch and seed were carried out using the OIV (Office International de la Vigne et du Vin) list for grapevine cultivars and <I>Vitis</I> species. However, several other parameters of the cultivars were measured for evaluation.
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Bessis, Roger. "Evolution of the grapevine (Vitis vinifera L.) imprinted by natural and human factorsThis review is one of a selection of papers presented at the symposium on Vitis at the XVII International Botanical Congress held in Vienna, Austria, in 2005." Canadian Journal of Botany 85, no. 8 (August 2007): 679–90. http://dx.doi.org/10.1139/b07-060.
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This paper is concerned with vegetative reproduction, which is the only mode of propagation in cultivated grapevines. After a brief summary of the systematics of the Vitaceae the question of the nomenclature of cultivated grapevines is discussed. Intra-varietal variability is discussed using morphological and molecular characteristics. The origins of variation are presented: mutations, foreign nucleic acids, and memory of previous environmental conditions. Grapevines provide characteristic products, leading viticulturalists to make selections among the diversity observed in the vineyard, thus decreasing diversity. Grapevines interact with various environmental agents. One example, the natural defence provided by resveratrol, is analysed, showing its direct effects against parasitic fungi and its indirect effects, both metabolic and genetic, on other organisms. Thus, the grapevine, viticulturalists, and their environment form a web of biological interactions.
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Monis, Judit, and Richard K. Bestwick. "Serological Detection of Grapevine Associated Closteroviruses in Infected Grapevine Cultivars." Plant Disease 81, no. 7 (July 1997): 802–8. http://dx.doi.org/10.1094/pdis.1997.81.7.802.
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Western blot immunoassay and enzyme-linked immunosorbent assay using different monoclonal antibodies (MAb) and polyclonal antisera (PA) revealed mixed infections of serologically related and unrelated grapevine leafroll associated viruses (GLRaVs) and grapevine corky bark associated virus (GCBaV) in symptomatic grapevines. A PA designated rootstock-scion incompatibility (RSI)-24 kDa, grapevine corky bark PA, and GLRaV-2b MAb reacted to polypeptides of approximately 24 kDa isolated from grapevines exhibiting rootstock-scion incompatibility, leafroll, and corky bark disease symptoms, suggesting that these isolates are infected with closely related viruses. A PA designated GLRaV-2 US detected virus specific polypeptides of 38, 37, 36, and 24 kDa, while a polyclonal antiserum designated GLRaV-2 FR detected a single virus-specific polypeptide of approximately 24 kDa. The reactivity of GLRaV-2 US to various polypeptides suggests that the immunogen used to produce this antiserum was a mixture of viruses. Apical meristems were excised and cultured to eliminate the infection of viruses in the grapevines showing RSI symptoms and in the cultivar French Colombard infected with GLRaV-1. The elimination of these viruses was confirmed by Western blot assay. These studies show that the Western blot assay can be used to detect and differentiate grapevine disease-associated closteroviruses.
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Petrovic, Natasa, Baozhong Meng, Maja Ravnikar, Irena Mavric, and Dennis Gonsalves. "First Detection of Rupestris stem pitting associated virus Particles by Antibody to a Recombinant Coat Protein." Plant Disease 87, no. 5 (May 2003): 510–14. http://dx.doi.org/10.1094/pdis.2003.87.5.510.
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Rupestris stem pitting associated virus (RSPaV), a member of the genus Foveavirus, is associated with the Rupestris stem pitting component of the Rugose wood (RW) disease complex of grapevines. Heretofore, particles of RSPaV have not been visualized. In this work, flexuous rod particles approximately 723 nm in length were detected in the sap of infected grapevines by immunosorbent electron microscopy (ISEM), using a polyclonal antiserum produced to a recombinant coat protein of RSPaV. Particles of RSPaV were detected in tissue culture-, greenhouse-, and field-grown grapevines infected with RSPaV, but not in healthy control plants. Detection of virus particles by ISEM corresponded with detection of RSPaV by Western blot, enzyme-linked immunosorbent assay, and reverse transcription-polymerase chain reaction. Virus particles were decorated with the antibodies specific to RSPaV but not with antibodies to Grapevine virus A or Grapevine virus B, two other viruses believed to be associated with RW. This definitive identification of RSPaV particles will help define the etiology of RW.
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Lopes, M. S., D. Mendonça, M. Rodrigues dos Santos, J. E. Eiras-Dias, and A. da Câmara Machado. "New insights on the genetic basis of Portuguese grapevine and on grapevine domestication." Genome 52, no. 9 (September 2009): 790–800. http://dx.doi.org/10.1139/g09-048.
Full textAbstract:
As the ancestor of cultivated grape, Vitis vinifera subsp. sylvestris represents a unique, invaluable genetic resource for the improvement of cultivated grapevines. Recently, five populations of wild grapevines were identified in Portugal. Sixty vines were characterized with 11 nuclear SSR markers and further compared with 70 genotypes of Portuguese Vitis vinifera subsp. sativa. The obtained data demonstrate moderate genetic differentiation between wild grapevine populations and moderate to high genetic differentiation between wild and cultivated grapevines. However, the identification of high degrees of similarity between wild and cultivated grapes (up to 87%) and a putative parent–progeny relationship between wild and cultivated grapes with 17 additional SSR markers is indicative of gene flow between local wild grapevine populations and Portuguese domesticated vines. Also, the ancestry of some Azorean cultivars was ascertained. The obtained data further support the hypothesis of several domestication centres, with Portugal, Spain, and Italy playing a particular role after the last glaciation, giving rise to many of the Western European cultivars.
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Dermastia, Marina, Blaž Škrlj, Rebeka Strah, Barbara Anžič, Špela Tomaž, Maja Križnik, Christina Schönhuber, et al. "Differential Response of Grapevine to Infection with ‘Candidatus Phytoplasma solani’ in Early and Late Growing Season through Complex Regulation of mRNA and Small RNA Transcriptomes." International Journal of Molecular Sciences 22, no. 7 (March 29, 2021): 3531. http://dx.doi.org/10.3390/ijms22073531.
Full textAbstract:
Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘Candidatus Phytoplasma solani’, but molecular interactions between the causal pathogen and its host plant are not well understood. In this work, we combined the analysis of high-throughput RNA-Seq and sRNA-Seq data with interaction network analysis for finding new cross-talks among pathways involved in infection of grapevine cv. Zweigelt with ‘Ca. P. solani’ in early and late growing seasons. While the early growing season was very dynamic at the transcriptional level in asymptomatic grapevines, the regulation at the level of small RNAs was more pronounced later in the season when symptoms developed in infected grapevines. Most differentially expressed small RNAs were associated with biotic stress. Our study also exposes the less-studied role of hormones in disease development and shows that hormonal balance was already perturbed before symptoms development in infected grapevines. Analysis at the level of communities of genes and mRNA-microRNA interaction networks revealed several new genes (e.g., expansins and cryptdin) that have not been associated with phytoplasma pathogenicity previously. These novel actors may present a new reference framework for research and diagnostics of phytoplasma diseases of grapevine.
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Moukarzel, Romy, Hayley J. Ridgway, Alexis Guerin-Laguette, and E. Eirian Jones. "An improved clearing and staining protocol for evaluation of arbuscular mycorrhizal colonisation in darkly pigmented woody roots." New Zealand Plant Protection 73 (November 15, 2020): 33–39. http://dx.doi.org/10.30843/nzpp.2020.73.11711.
Full textAbstract:
Arbuscular mycorrhizal fungi (AMF) establish symbiotic interactions with the roots of vascular plants, including grapevines. Verifying AMF colonisation routinely requires establishing the presence of hyphae, arbuscules and vesicles. Clearing roots with potassium hydroxide (KOH) followed by staining with trypan blue has been used previously to visualise fungal structures, however visualisation is difficult with darkly pigmented roots, such as those of grapevines so additional steps are required to ensure clear visualisation. Three fixing and clearing processes were evaluated prior to staining with trypan blue: 1) fixing grapevine roots in 70% v/v ethanol overnight; 2) clearing by heating the roots in either 2% or 10% w/v KOH; and 3) clearing the roots in 3% v/v hydrogen peroxide for 10 min. Roots were examined under a compound light microscope for the presence of AMF. A combination of fixing grapevine roots in 70% ethanol overnight and clearing by autoclaving in 10% KOH produced the greatest enhancement in subsequent staining of grapevine roots with trypan blue overnight. The best method tested enabled the discrimination of arbuscular mycorrhizal structures in fresh roots of grapevines without the use of toxic chemical fixatives.
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Alaniz, Sandra, Josep Armengol, José García-Jiménez, Paloma Abad-Campos, and Maela León. "A Multiplex PCR System for the Specific Detection of Cylindrocarpon liriodendri, C. macrodidymum, and C. pauciseptatum from Grapevine." Plant Disease 93, no. 8 (August 2009): 821–25. http://dx.doi.org/10.1094/pdis-93-8-0821.
Full textAbstract:
Cylindrocarpon liriodendri and C. macrodidymum are the causal agents of grapevine black foot disease. Recently, a third species, C. pauciseptatum, has been isolated from roots of grapevine showing decline symptoms. Currently, reliable identification of isolates of these species through phenotypical characteristics has not been possible. The polymerase chain reaction (PCR)-based method developed in this study allows a quick and easy detection of Cylindrocarpon spp. associated with grapevine. Three primer pairs annealing to variable, partly species-specific sites of the internal transcribed spacer regions amplified species-specific PCR fragments of different sizes in C. liriodendri, C. macrodidymum, and C. pauciseptatum in a multiplex assay with DNA obtained with both quick and traditional extraction methods. They did not generate any PCR product in other fungal trunk pathogens or contaminants commonly associated with grapevines. When universal fungal ITS primers were used in a nested multiplex PCR, the three primer pairs also detected C. liriodendri, C. macrodidymum, and C. pauciseptatum in total DNA extracted from roots of inoculated grapevines. The designed methods can be used for the diagnosis of these fungi from pure culture or infected grapevines.
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Mirchenari, Seyed Mehdi, Amir Massah, and Leila Zirak. "‘Bois noir’: new phytoplasma disease of grapevine in Iran." Journal of Plant Protection Research 55, no. 1 (January 1, 2015): 88–93. http://dx.doi.org/10.1515/jppr-2015-0012.
Full textAbstract:
Abstract Recently, grapevines showing symptoms suggesting the ‘bois noir’ phytoplasma disease were observed in vineyards located in several central provinces of Iran. Polymerase chain reaction assays using phytoplasma universal primer pair P1A/P7A followed by primer pair R16F2n/R16R2 in nested PCR, confirmed the association of phytoplasmas with symptomatic grapevines. The results of RFLP analyses using HpaII, HinfI, MseI, RsaI, and TaqI restriction enzymes, indicated that grapevine phytoplasma isolates in these regions could be related to the 16SrXII group. Sequence analyses of the partial 16S rRNA gene confirmed that Iranian grapevine phytoplasmas are associated with ‘Candidatus Phytoplasma solani’. This is the first report of the ‘bois noir’ disease outbreak in Iran
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Jež-Krebelj, Anastazija, Maja Rupnik-Cigoj, Marija Stele, Marko Chersicola, Maruša Pompe-Novak, and Paolo Sivilotti. "The Physiological Impact of GFLV Virus Infection on Grapevine Water Status: First Observations." Plants 11, no. 2 (January 7, 2022): 161. http://dx.doi.org/10.3390/plants11020161.
Full textAbstract:
In a vineyard, grapevines are simultaneously exposed to combinations of several abiotic (drought, extreme temperatures, salinity) and biotic stresses (phytoplasmas, viruses, bacteria). With climate change, the incidences of drought in vine growing regions are increased and the host range of pathogens with increased chances of virulent strain development has expanded. Therefore, we studied the impact of the combination of abiotic (drought) and biotic (Grapevine fanleaf virus (GFLV) infection) stress on physiological and molecular responses on the grapevine of cv. Schioppettino by studying the influence of drought and GFLV infection on plant water status of grapevines, on grapevine xylem vessel occlusion, and on expression patterns of 9-cis-epoxycarotenoid dioxygenase 1 (NCED1), 9-cis-epoxycarotenoid dioxygenase 2 (NCED2), WRKY encoding transcription factor (WRKY54) and RD22-like protein (RD22) genes in grapevines. A complex response of grapevine to the combination of drought and GFLV infection was shown, including priming in the case of grapevine water status, net effect in the case of area of occluded vessels in xylem, and different types of interaction of both stresses in the case of expression of four abscisic acid-related genes. Our results showed that mild (but not severe) water stress can be better sustained by GFLV infection rather than by healthy vines. GFLV proved to improve the resilience of the plants to water stress, which is an important outcome to cope with the challenges of global warming.
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Soltani, Nourolah, Rongbin Hu, Darrell D. Hensley, David L. Lockwood, Keith L. Perry, and M. R. Hajimorad. "A Survey for Nine Major Viruses of Grapevines in Tennessee Vineyards." Plant Health Progress 21, no. 3 (January 1, 2020): 157–61. http://dx.doi.org/10.1094/php-03-20-0018-rs.
Full textAbstract:
Despite the significance of grape production to the fruit industry in Tennessee (TN), no published information has been available on viruses affecting grapevines in the state. Hence, a survey was conducted during the 2016 and 2017 growing seasons to determine the status of nine major viruses of grapevines in TN vineyards by taking advantage of classical serological assays and confirmatory nucleic acid-based diagnostic approaches. A total of 349 samples from 23 grapevine cultivars mostly displaying viral-like symptoms were collected from 23 commercial vineyards. All samples were assayed by DAS-ELISA for arabis mosaic virus (ArMV), grapevine leafroll-associated virus (GLRaV)-1, GLRaV-2, GLRaV-3, GLRaV-4, grapevine fanleaf virus (GFLV), tobacco ringspot virus (TRSV), and tomato ringspot virus (ToRSV). Selected serologically positive samples were also tested by RT-PCR, followed by Sanger sequencing of the generated amplicons. Additionally, 19 grapevines displaying symptoms characteristic of grapevine red blotch virus (GRBV) were also assayed by PCR followed by confirmatory sequencing-based methods. Collectively, these assays verifiably detected GLRaV-1, GLRaV-2, GLRaV-3, ToRSV, and GRBV in TN vineyards. This is the first record of the presence of these viruses in TN vineyards. ArMV, GLRaV-4, GFLV, and TRSV were not detected. The majority of samples tested positive for a single virus, whereas mixed infections with more than one virus were detected in 37% of samples.
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Rozane, Danilo Eduardo, Betania Vahl de Paula, George Wellington Bastos de Melo, Eduardo Maciel Haitzmann dos Santos, Edicarla Trentin, Carina Marchezan, Lincon Oliveira Stefanello da Silva, et al. "Compositional Nutrient Diagnosis (CND) Applied to Grapevines Grown in Subtropical Climate Region." Horticulturae 6, no. 3 (September 4, 2020): 56. http://dx.doi.org/10.3390/horticulturae6030056.
Full textAbstract:
Soil analysis is used to estimate nutrient availability, but nutrient concentrations are not always related to yield in most fruit plants, including grapevines. Thus, additional multivariate mathematical models, such as the compositional nutrient diagnosis (CND), which takes into account leaves nutrient concentration, and yield, can contribute to estimating critical levels or sufficiency bands of elements, as well as to detect deficiency and/or excess of nutrients. The aim of the present study was to establish CND standards, and the critical level and sufficiency band of nutrients, in the grapevine Vitis vinifera L., grown in a subtropical climate region. Leaves were collected in 81 vineyards in the Campanha Gaúcha do Rio Grande do Sul region, Southern Brazil, and analyzed for macro- and micro-nutrient concentration. The yield of each vineyard was assessed. Grapevine nutritional status was calculated through the CND method. CND-r2 indices were effective in establishing the nutritional status of grapevines for macro- and micro-nutrients as sub-optimal, excessive, or balanced. The CND methodology established the critical level and sufficiency bands of nutrients more accurately than the current recommendations for grapevines. Multi-nutrient associations were more effective than the single nutrient determination in defining the threshold of a given nutrient that can reduce grapevine yield.
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Dinis, Lia-Tânia, Cláudia Jesus, Joana Amaral, Aurelio Gómez-Cadenas, Barbara Correia, Artur Alves, and Glória Pinto. "Water Deficit Timing Differentially Affects Physiological Responses of Grapevines Infected with Lasiodiplodia theobromae." Plants 11, no. 15 (July 28, 2022): 1961. http://dx.doi.org/10.3390/plants11151961.
Full textAbstract:
Diseases and climate change are major factors limiting grape productivity and fruit marketability. Lasiodiplodia theobromae is a fungus of the family Botryosphaeriaceae that causes Botryosphaeria dieback of grapevine worldwide. Abiotic stress may change host vitality and impact susceptibility to the pathogen and/or change the pathogen’s life cycle. However, the interaction between both stress drivers is poorly understood for woody plants. We addressed the hypothesis that distinct morpho-physiological and biochemical responses are induced in grapevine (Vitis vinifera)–L. theobromae interactions depending on when water deficits are imposed. Grapevines were submitted to water deficit either before or after fungus inoculation. Water deficit led to the reduction of the net photosynthetic rate, stomatal conductance, and transpiration rate, and increased the abscisic acid concentration regardless of fungal inoculation. L. theobromae inoculation before water deficit reduced plant survival by 50% and resulted in the accumulation of jasmonic acid and reductions in malondialdehyde levels. Conversely, grapevines inoculated after water deficit showed an increase in proline and malondialdehyde content and all plants survived. Overall, grapevines responded differently to the primary stress encountered, with consequences in their physiological responses. This study reinforces the importance of exploring the complex water deficit timing × disease interaction and the underlying physiological responses involved in grapevine performance.
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Garcia, Shahinez, Jean-Michel Hily, Véronique Komar, Claude Gertz, Gérard Demangeat, Olivier Lemaire, and Emmanuelle Vigne. "Detection of Multiple Variants of Grapevine Fanleaf Virus in Single Xiphinema index Nematodes." Viruses 11, no. 12 (December 10, 2019): 1139. http://dx.doi.org/10.3390/v11121139.
Full textAbstract:
Grapevine fanleaf virus (GFLV) is responsible for a widespread disease in vineyards worldwide. Its genome is composed of two single-stranded positive-sense RNAs, which both show a high genetic diversity. The virus is transmitted from grapevine to grapevine by the ectoparasitic nematode Xiphinema index. Grapevines in diseased vineyards are often infected by multiple genetic variants of GFLV but no information is available on the molecular composition of virus variants retained in X. index following nematodes feeding on roots. In this work, aviruliferous X. index were fed on three naturally GFLV-infected grapevines for which the virome was characterized by RNAseq. Six RNA-1 and four RNA-2 molecules were assembled segregating into four and three distinct phylogenetic clades of RNA-1 and RNA-2, respectively. After 19 months of rearing, single and pools of 30 X. index tested positive for GFLV. Additionally, either pooled or single X. index carried multiple variants of the two GFLV genomic RNAs. However, the full viral genetic diversity found in the leaves of infected grapevines was not detected in viruliferous nematodes, indicating a genetic bottleneck. Our results provide new insights into the complexity of GFLV populations and the putative role of X. index as reservoirs of virus diversity.
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Costa, H. S., E. Raetz, T. R. Pinckard, C. Gispert, R. Hernandez-Martinez, C. K. Dumenyo, and D. A. Cooksey. "Plant Hosts of Xylella fastidiosa In and Near Southern California Vineyards." Plant Disease 88, no. 11 (November 2004): 1255–61. http://dx.doi.org/10.1094/pdis.2004.88.11.1255.
Full textAbstract:
Xylella fastidiosa is a xylem-limited bacterium that causes Pierce's disease (PD) of grapevines. A variety of plant species found near a severe outbreak of PD in vineyards in the Temecula Valley of California were tested using enzyme-linked immunosorbent assay, culture on media, and polymerase chain reaction to identify potential inoculum sources in the area. Species that consistently tested positive for X. fastidiosa were the known hosts, grape, almond, and oleander, and two new hosts, Spanish broom (Spartium junceum) and wild mustard (Brassica spp). Sequence analysis of the 16S-23S rRNA spacer region found that strains isolated from grapevine, Spanish broom, wild mustard, and almond clustered with previously sequenced PD strains. Thus, these species could serve as sources of inoculum for infection of grapevines and should be removed or monitored for signs of infection. Sequences from oleander isolates from Temecula formed another cluster with a previously published oleander strain sequence. Oleander strains do not infect grapevines and thus do not appear to cause a direct threat to grapevines. Two additional isolates from almond were determined to be genetically different from PD strains, and the ability of these strains to infect grapevine is not known. Greenhouse transmission studies indicate that the glassy-winged sharpshooter was able to transmit a PD strain of X. fastidiosa to Spanish broom, black mustard, and other hosts.
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Bahder, Brian W., Frank G. Zalom, Maya Jayanth, and Mysore R. Sudarshana. "Phylogeny of Geminivirus Coat Protein Sequences and Digital PCR Aid in Identifying Spissistilus festinus as a Vector of Grapevine red blotch-associated virus." Phytopathology® 106, no. 10 (October 2016): 1223–30. http://dx.doi.org/10.1094/phyto-03-16-0125-fi.
Full textAbstract:
Grapevine red blotch-associated virus (GRBaV) is a single-stranded DNA virus, proposed to be a member of the family Geminiviridae, and is associated with grapevines showing red blotch symptoms in North America. The existence of the virus was reported in 2012, and subsequently detected in grapevines in major grape production regions. We investigated if a vector exists that can transmit GRBaV in vineyards. Phylogenetic analysis of the predicted amino acid sequence of coat protein (CP) of GRBaV with the CP of 23 geminiviruses representing all seven genera of the family Geminiviridae revealed that GRBaV-CP was most similar to that of Tomato pseudo-curly top virus, a geminivirus known to be transmitted by a treehopper (Membracidae), a family that is closely related to leafhoppers (Cicadellidae). To identify vectors of GRBaV, hemipteran species within and nearby wine grape vineyards where virus spread was suspected were collected and transmission assays were conducted. Among the species tested, the three-cornered alfalfa hopper Spissistilus festinus (Hemiptera: Membracidae) was able to both acquire the virus from a grapevine infected with GRBaV and transmit the virus to healthy grapevines in the laboratory. In commercial vineyards, lateral shoots of grapevines girdled due to feeding injury by the adult three-cornered alfalfa hopper also tested positive for the virus using digital PCR. These findings represent an important step in understanding the biology of GRBaV and develop management guidelines.
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Ye, Qiuhong, Hua Wang, and Hua Li. "Arbuscular Mycorrhizal Fungi Improve Growth, Photosynthetic Activity, and Chlorophyll Fluorescence of Vitis vinifera L. cv. Ecolly under Drought Stress." Agronomy 12, no. 7 (June 29, 2022): 1563. http://dx.doi.org/10.3390/agronomy12071563.
Full textAbstract:
Drought stress has become a limiting factor for viticulture with climate change. The influence of arbuscular mycorrhizal fungi (AMF) on grapevine Vitis vinifera L. cv. Ecolly’s leaf water content, chlorophyll concentration, photosynthesis activity, and chlorophyll fluorescence under drought stress was studied in the greenhouse. The experiment was designed as a randomized complete block with four treatments: AMF colonization, well-watered; non-AMF colonization, well-watered; AMF colonization with drought stress; and non-AMF colonization with drought stress. The grapevines inoculated with mycorrhiza had a higher water content in the leaves and higher chlorophyll concentration under drought stress than those without mycorrhiza inoculation. AMF colonization increased the dry biomass of shoots and roots, photosynthetic rate, stomatal conductance, and transpiration rate and decreased intercellular CO2 concentration. Mycorrhizal grapevines had higher non-photochemistry efficiency, higher photochemistry efficiency, and higher actual quantum yield than non-mycorrhizal grapevines. The results show that AMF alleviated the negative effects of drought stress on grapevines. The alleviation improved leaf water status, chlorophyll concentration, and photosynthetic capacity. Altogether, the results of our study indicate that AMF inoculation has the potential to protect grapevines under drought stress.
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Moyo, Providence, Lizel Mostert, Christoffel F. J. Spies, Ulrike Damm, and Francois Halleen. "Diversity of Diatrypaceae Species Associated with Dieback of Grapevines in South Africa, with the Description of Eutypa cremea sp. nov." Plant Disease 102, no. 1 (January 2018): 220–30. http://dx.doi.org/10.1094/pdis-05-17-0738-re.
Full textAbstract:
Recent studies in grape-growing areas including Australia, California, and Spain have revealed an extensive diversity of Diatrypaceae species on grapevines showing dieback symptoms and cankers. However, in South Africa, little is known regarding the diversity of these species in vineyards. The aim of this study was, therefore, to identify and characterize Diatrypaceae species associated with dieback symptoms of grapevine in South Africa. Isolates were collected from dying spurs of grapevines aged 4 to 8 years old, grapevine wood showing wedge-shaped necrosis when cut in cross section as well as from perithecia on dead grapevine wood. The collected isolates were identified based on morphological characters and phylogenetic analyses of the internal transcribed spacer region (ITS) and β-tubulin gene. Seven Diatrypaceae species were identified on grapevine, namely Cryptovalsa ampelina, C. rabenhorstii, Eutypa consobrina, E. lata, E. cremea sp. nov., Eutypella citricola, and E. microtheca. The dying spurs yielded the highest diversity of species when compared with the wedge-shaped necrosis and/or perithecia. C. ampelina was the dominant species in the dying spurs, followed by E. citricola, whereas E. lata was the dominant species isolated from the wedge-shaped necroses and perithecia. These results confirm E. lata as an important grapevine canker pathogen in South Africa, but the frequent association of C. ampelina with spur dieback suggests that this pathogen plays a more prominent role in dieback than previously assumed. In some cases, more than one species were isolated from a single symptom, which suggests that interactions may be occurring leading to decline of grapevines. C. rabenhorstii, E. consobrina, E. citricola, E. microtheca, and E. cremea are reported for the first time on grapevine in South Africa.
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Rwahnih, Maher Al, Ashita Dave, Michael M. Anderson, Adib Rowhani, Jerry K. Uyemoto, and Mysore R. Sudarshana. "Association of a DNA Virus with Grapevines Affected by Red Blotch Disease in California." Phytopathology® 103, no. 10 (October 2013): 1069–76. http://dx.doi.org/10.1094/phyto-10-12-0253-r.
Full textAbstract:
In the Napa Valley of California, vineyards of ‘Cabernet Franc’ (CF) clone 214, ‘Cabernet Sauvignon’ clone 337, and ‘Zinfandel’ clone 1A (Z1A) with grapevines exhibiting foliar symptoms of red blotches, marginal reddening, and red veins that were accompanied by reduced sugar accumulation in fruit at harvest were initially suspected to be infected with leafroll-associated viruses. However, reverse-transcription polymerase chain reaction (PCR) tests were negative for all known leafroll-associated viruses, with the exception of Grapevine leafroll-associated virus 2 in Z1A. Metagenomic analysis of cDNA libraries obtained from double-stranded RNA enriched nucleic acid (NA) preparations from bark scrapings of dormant canes on an Illumina platform revealed sequences having a distant relationship with members of the family Geminiviridae. Sequencing of products obtained by PCR assays using overlapping primers and rolling circle amplification (RCA) confirmed the presence of a single circular genome of 3,206 nucleotides which was nearly identical to the genome of a recently reported Grapevine cabernet franc-associated virus found in declining grapevines in New York. We propose to call this virus “Grapevine red blotch-associated virus” (GRBaV) to describe its association with grapevine red blotch disease. Primers specific to GRBaV amplified a product of expected size (557 bp) from NA preparations obtained from petioles of several diseased source vines. Chip bud inoculations successfully transmitted GRBaV to test plants of CF, as confirmed by PCR analysis. This is the first report of a DNA virus associated with red blotch disease of grapevines in California.
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Abeywickrama, Pranami, Wei Zhang, Xinghong Li, Ruvishika Jayawardena, Kevin Hyde, and Jiye Yan. "Campylocarpon fasciculare (Nectriaceae, Sordariomycetes); Novel Emergence of Black-Foot Causing Pathogen on Young Grapevines in China." Pathogens 10, no. 12 (November 29, 2021): 1555. http://dx.doi.org/10.3390/pathogens10121555.
Full textAbstract:
The black-foot disease of grapevine is one of the most destructive diseases in viticulture and it is caused by a complex of soil-borne fungi. This study aimed to identify the species associated with black-foot disease in young grapevines in vineyards of China. Fungal isolates were identified as Campylocarpon fasciculare, based on both morphological and multi-locus phylogenetic analysis of ITS, tef1–α and ß-tubulin sequence data. For the first time in China, we report Campylocarpon fasciculare associated with symptomatic young grapevines. Koch’s postulates were performed on Vitis vinifera cv. Summer Black (SB) in a greenhouse and to confirm the pathogenicity on grapevines. This work improves the knowledge of black-foot disease in Chinese vineyards and will be helpful to growers in their decisions regarding vinicultural practices, planting and disease management.
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Gutiérrez-Gamboa, Gastón, Teresa Garde-Cerdán, Bianca Souza-Da Costa, and Yerko Moreno-Simunovic. "Strategies for the improvement of fruit set in Vitis vinifera L. cv. ‘Carménère’ through different foliar biostimulants in two different locations." Ciência e Técnica Vitivinícola 33, no. 2 (2018): 177–83. http://dx.doi.org/10.1051/ctv/20183302177.
Full textAbstract:
‘Carménère’ is an important grapevine variety cultivated in Chile, which exhibits a high tendency to fruitlet abscission and “millerandage”, reproductive disorders that seriously affect plant productivity. However, despite the existence of a lot of information about the effects of micronutrients on millerandage, few studies exist about the impacts of seaweed biostimulation on reproductive disorders in grapevines, which currently has been classified as elicitor. For these reasons, the aim of this work was to study the effect of different biostimulant applications to ‘Carménère’ grapevines on fruit set and cluster characteristics during 2011-2012 season. Viticultural parameters such as number and weight of berries, cluster weight, berry size and its distribution along the cluster were measured. In addition, physicochemical parameters in musts from the obtained grapevines were analyzed. The results showed that the treatments based on boron ethanolamine plus zinc oxide and brown algae (Ascophyllum nodosum) improved fruit set in ‘Carménère’ grapevines in both climate locations, mainly in the colder climate site. Different parameters such as weight and number of berries per bunch, among others, were improved after the application of this treatment. Therefore, the results suggest that it is possible to improve fruit set in ‘Carménère’ grapevines using a treatment based on certain microelements, such as boron and zinc, and an extract produced from seaweed algae.
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Karácsony, Zoltán, Dániel G. Knapp, Szabina Lengyel, Gábor M. Kovács, and Kálmán Zoltán Váczy. "The fungus Kalmusia longispora is able to cause vascular necrosis on Vitis vinifera." PLOS ONE 16, no. 10 (October 15, 2021): e0258043. http://dx.doi.org/10.1371/journal.pone.0258043.
Full textAbstract:
Fungal diseases in agronomically important plants such as grapevines result in significantly reduced production, pecuniary losses, and increased use of environmentally damaging chemicals. Beside the well-known diseases, there is an increased interest in wood-colonizing fungal pathogens that infect the woody tissues of grapevines. In 2015, a traditional isolation method was performed on grapevine trunks showing symptoms of trunk diseases in Hungary. One isolate (T15142) was identified as Kalmusia longispora (formerly Dendrothyrium longisporum) according to morphological and phylogenetic analyses. To evaluate the pathogenicity of this fungus on grapevines, artificial infections were carried out under greenhouse and field conditions, including the CBS 824.84 and ex-type CBS 582.83 strains. All isolates could be re-isolated from inoculated plants; however, varying virulence was observed among them in terms of the vascular necrosis caused. The incidence and severity of this symptom seemed to be congruent with the laccase-producing capabilities of the isolates. This is the first report on the ability of Kalmusia longispora to cause symptoms on grapevines, and on its possible dependence on laccase secretion.
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Pádua, Luís, Telmo Adão, António Sousa, Emanuel Peres, and Joaquim J. Sousa. "Individual Grapevine Analysis in a Multi-Temporal Context Using UAV-Based Multi-Sensor Imagery." Remote Sensing 12, no. 1 (January 1, 2020): 139. http://dx.doi.org/10.3390/rs12010139.
Full textAbstract:
The use of unmanned aerial vehicles (UAVs) for remote sensing applications in precision viticulture significantly increased in the last years. UAVs’ capability to acquire high spatiotemporal resolution and georeferenced imagery from different sensors make them a powerful tool for a better understanding of vineyard spatial and multitemporal heterogeneity, allowing the estimation of parameters directly impacting plants’ health status. In this way, the decision support process in precision viticulture can be greatly improved. However, despite the proliferation of these innovative technologies in viticulture, most of the published studies rely only on data from a single sensor in order to achieve a specific goal and/or in a single/small period of the vineyard development. In order to address these limitations and fully exploit the advantages offered by the use of UAVs, this study explores the multi-temporal analysis of vineyard plots at a grapevine scale using different imagery sensors. Individual grapevine detection enables the estimation of biophysical and geometrical parameters, as well as missing grapevine plants. A validation procedure was carried out in six vineyard plots focusing on the detected number of grapevines and missing grapevines. A high overall agreement was obtained concerning the number of grapevines present in each row (99.8%), as well as in the individual grapevine identification (mean overall accuracy of 97.5%). Aerial surveys were conducted in two vineyard plots at different growth stages, being acquired for RGB, multispectral and thermal imagery. Moreover, the extracted individual grapevine parameters enabled us to assess the vineyard variability in a given epoch and to monitor its multi-temporal evolution. This type of analysis is critical for precision viticulture, constituting as a tool to significantly support the decision-making process.
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Probst, Chantal M., Hayley J. Ridgway, Marlene V. Jaspers, and E. Eirian Jones. "Pathogenicity of Ilyonectria pseudodestructans propagules to grapevine rootstocks." New Zealand Plant Protection 72 (July 26, 2019): 1–9. http://dx.doi.org/10.30843/nzpp.2019.72.314.
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Black foot disease of grapevines is a major economic issue for the viticulture industry, with several Dactylonectria and Ilyonectria species identified as causal agents worldwide. This study aimed to confirm the pathogenicity of an Ilyonectria pseudodestructans isolate recovered from a symptomatic grapevine in a nationwide survey. An initial pot experiment inoculated callused and root-wounded grapevine propagation material of varieties ‘101- 14’ and ‘5C’ with I. pseudodestructans conidia. The second pot experiment compared the pathogenicity of I. pseudodestructans conidial, chlamydospore and mycelial inocula. Disease incidence, severity and root and shoot dry weights were determined after 4–5 months of growth. Ilyonectria pseudodestructans was recovered from inoculated plants resulting in higher disease incidence and severity compared with the uninoculated control. Disease severity and incidence was higher for callused compared to rooted propagation material, but did not differ between grapevine varieties. Conidial inoculum caused greater disease incidence and severity compared with chlamydospore and mycelial inocula. Ilyonectria pseudodestructans propagules infected grapevine plant material via the callused basal ends or wounded roots, indicating this species is a potentially important pathogen of grapevines both in nurseries and vineyards.
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Purcell, Alexander H., and Stuart R. Saunders. "Glassy-Winged Sharpshooters Expected to Increase Plant Disease." Plant Health Progress 1, no. 1 (January 2000): 3. http://dx.doi.org/10.1094/php-2000-0627-02-rs.
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As it moves through California, the new pest known as glassy-winged sharpshooter (GWSS) may significantly increase the spread of plant diseases caused by the bacterium Xylella fastidiosa. The bacterium causes Pierce's disease of grapevines, almond leaf scorch and the newly recognized oleander leaf scorch disease. Currently, almond leaf scorch is a minor problem for California's almonds, but if GWSS becomes established in significant numbers in or near almond orchards, it might increase the incidence of almond leaf scorch. Our studies show that GWSS and two other sharpshooter species can transmit X. fastidiosa from diseased oleanders to healthy oleanders. GWSS also can transmit Pierce's disease strains of the bacterium from grapevine to grapevine and to almond trees. The oleander strain of the bacterium did not infect grapevines, but the ability of GWSS to transmit Pierce's disease strains may increase the spread of this lethal grapevine disease in vineyards bordering citrus orchards or other habitats where invading GWSS may establish permanent populations. Posted 27 December 2000.
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Stewart, Elwin L., Xinshun Qu, Barrie E. Overton, Fred E. Gildow, Nancy G. Wenner, and Deborah S. Grove. "Development of a Real-Time RT-PCR SYBR Green Assay for Tomato ring spot virus in Grape." Plant Disease 91, no. 9 (September 2007): 1083–88. http://dx.doi.org/10.1094/pdis-91-9-1083.
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Grapevines infected with Tomato ring spot virus (ToRSV) pose an economic risk for growers in the northeastern United States. This study describes a one-step real-time reverse-transcription polymerase chain reaction (RT-PCR) SYBR Green assay for detecting ToRSV in grapevines. Two newly designed primer pairs based on the ToRSV coat protein gene sequence were evaluated for specificity and optimized for a SYBR Green assay. The primer pair ToRSV1f/1r yielded a 130-bp product with strong primer-dimer products, whereas the primer pair ToRSV2f/2r yielded a 330-bp product with weak primer dimer products. Real-time RT-PCR detected ToRSV in more naturally infected grapevines maintained in the greenhouse than did enzyme-linked immunosorbent assay. The nucleotide sequences of the fragments amplified from grapevine growing in Pennsylvania using real-time PCR were divergent from previously published sequences.
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Sisterson, Mark S., Lindsey P. Burbank, Rodrigo Krugner, David Haviland, and Drake C. Stenger. "Xylella fastidiosa and Glassy-Winged Sharpshooter Population Dynamics in the Southern San Joaquin Valley of California." Plant Disease 104, no. 11 (November 2020): 2994–3001. http://dx.doi.org/10.1094/pdis-01-20-0066-re.
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Xylella fastidiosa is a vector-transmitted bacterial plant pathogen that affects a wide array of perennial crops, including grapevines (Pierce’s disease). In the southern San Joaquin Valley of California, epidemics of Pierce’s disease of grapevine were associated with the glassy-winged sharpshooter, Homalodisca vitripennis. During the growing season, rates of X. fastidiosa spread in vineyards are affected by changes in pathogen distribution within chronically infected grapevines and by vector population dynamics. Grapevines chronically infected with X. fastidiosa rarely tested positive for the pathogen prior to July, suggesting vector acquisition of X. fastidiosa from grapevines increases as the season progresses. This hypothesis was supported by an increase in number of X. fastidiosa-positive glassy-winged sharpshooters collected from vineyards during July through September. Analysis of insecticide records indicated that vineyards in the study area were typically treated with a systemic neonicotinoid in spring of each year. As a result, abundance of glassy-winged sharpshooters was typically low in late spring and early summer, with abundance of glassy-winged sharpshooter adults increasing in late June and early July of each year. Collectively, the results suggest that late summer is a crucial time for X. fastidiosa secondary spread in vineyards in the southern San Joaquin Valley, because glassy-winged sharpshooter abundance, number of glassy-winged sharpshooters testing positive for X. fastidiosa, and grapevines with detectable pathogen populations were all greatest during this period.
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Nebish, Anna, Javier Tello, Yolanda Ferradás, Rouben Aroutiounian, José Miguel Martínez-Zapater, and Javier Ibáñez. "SSR and SNP genetic profiling of Armenian grape cultivars gives insights into their identity and pedigree relationships." OENO One 55, no. 4 (November 10, 2021): 101–14. http://dx.doi.org/10.20870/oeno-one.2021.55.4.4815.
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The South Caucasus is recognised as the primary Vitis vinifera L. (grapevine) domestication centre and has a high diversity of wild and cultivated grapevines. Archaeological findings indicate that winemaking activities have existed in Armenia for more than 6,000 years, viticulture being one of the most important activities of the modern Armenian agricultural sector. Despite this relevance, some grapevines in local collections have not yet been properly identified, thus hindering the efficient conservation, characterisation and eventual use of autochthonous genetic resources. In the present study, a combined SNP and SSR profiling strategy was used for the genetic identification of a series of grapevine accessions from the Grape Collection of the International Academy of Viticulture and Winemaking in Nalbandyan, presumed to be autochthonous Armenian varieties. The results provided useful information for the correct identification of these genetic resources, revealing multiple cases of synonyms, homonyms and misnames. The genetic data made it possible to confirm the pedigree proposed for some of the cultivars identified in this study and to clarify the origin of others. In addition, we propose, for the first time, a series of new trios and duos involving autochthonous Armenian grapevines. The singularity of this genetic pool compared to other Western and Central European varieties, as well as the potential novel sources of variability in traits of interest (e.g., seedlessness) that were found, highlight the importance of improving knowledge of the Armenian grapevine genetic pool.
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M'hirsi, S., S. Fattouch, H. Acheche, M. Marrakchi, and N. Marzouki. "Detection of Grapevine A vitivirus in Tunisian grapevines." EPPO Bulletin 31, no. 4 (December 2001): 509–13. http://dx.doi.org/10.1111/j.1365-2338.2001.tb01035.x.
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Barceló, Alfonso Ros, Federico Pomar, Matías López-Serrano, and Maria Angeles Pedreño. "Peroxidase: a multifunctional enzyme in grapevines." Functional Plant Biology 30, no. 6 (2003): 577. http://dx.doi.org/10.1071/fp02096.
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Peroxidases are heme-containing enzymes that catalyse the one-electron oxidation of several substrates at the expense of H2O2. They are probably encoded by a large multigene family in grapevines, and therefore show a high degree of polymorphism. Grapevine peroxidases are glycoproteins of high thermal stability, whose molecular weight usually ranges from 35 to 45 kDa. Their visible spectrum shows absorption bands characteristic of high-spin class III peroxidases. Grapevine peroxidases are capable of accepting a wide range of natural compounds as substrates, such as the cell wall protein extensin, plant growth regulators such as IAA, and phenolics such as benzoic acids, stilbenes, flavonols, cinnamyl alcohols and anthocyanins. They are located in cell walls and vacuoles. These locations are in accordance with their key role in determining the final cell wall architecture, especially regarding lignin deposition and extensin insolubilization, and the turnover of vacuolar phenolic metabolites, a task that also forms part of the molecular program of disease resistance. Although peroxidase is a constitutive enzyme in grapevines, its levels are strongly modulated during plant cell development and in response to both biotic and abiotic environmental factors. To gain an insight into the metabolic regulation of peroxidase, several authors have studied how grapevine peroxidase and H2O2 levels change in response to a changing environment. Nevertheless, the results obtained are not always easy to interpret. Despite such difficulties, the response of the peroxidase–H2O2 system to both UV-C radiation and Trichoderma viride elicitors is worthy of study. Both UV-C and T. viride elicitors induce specific changes in peroxidase isoenzyme / H2O2 levels, which result in specific changes in grapevine physiology and metabolism. In the case of T. viride-elicited grapevine cells, they show a particular mechanism for H2O2 production, in which NADPH oxidase-like activities are apparently not involved. However, they offer a unique system whereby the metabolic regulation of peroxidase by H2O2, with all its cross-talks and downstream signals, may be elegantly dissected.
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Morales, R. Z., and J. Monis. "First Detection of Grapevine leafroll associated virus-7 in California Vineyards." Plant Disease 91, no. 4 (April 2007): 465. http://dx.doi.org/10.1094/pdis-91-4-0465b.
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Grapevine leafroll is one of the most important viral diseases in grapevine and occurs wherever grapevines are grown. At least nine different Grapevine leafroll associated viruses (GLRaVs) belonging to different genera within the Closteroviridae family have been reported. Typical leafroll symptoms include downward rolling and interveinal reddening or yellowing of leaves, reduced fruit size, sugar content, and yield. Our laboratory has detected the presence of GLRaV-7 in many grapevine cuttings collected in California vineyards. During the 2005-2006 fall/winter testing season, symptomatic and asymptomatic grapevine varieties collected from different vineyards in California were tested for the detection of economically important viruses and processed for reverse transcription (RT)-PCR and ELISA. The RT-PCR assays using primers specifically designed for the detection of GLRaV-7 (C. Turturo et al., 13th ICVG [Abstr.], 2000) amplified a single band of approximately 200 bp in samples from selections of Chardonnay, Merlot, Pinot Noir, and Sauvignon Blanc cultivars. The same samples showed low or no reactivity to GLRaV-7 specific antibodies (BIOREBA AG, Reinach, Switzerland). The variability of detection by ELISA can be due to the lower sensitivity of ELISA as compared with RT-PCR and the uneven distribution of GLRaVs in grapevines (1). To confirm that the amplified DNA was GLRaV-7 specific, different isolates were sequenced and compared with the nucleotide and derived amino acid sequences obtained by Turturo et al. (13th ICVG [Abstr.], 2000). The California isolates showed 81 to 98% similarity with the Italian isolates. To our knowledge, this is the first report of GLRaV-7 in California and the United States. Our initial testing data indicates that GLRaV-7 is wide spread in California vineyards. Further studies will allow us to determine the potential effect of this virus in grapevines grown in California. Reference: (1) J. Monis, and R. K. Bestwick. Am. J. Enol. Vitic. 47:199, 1996.
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Margaria, P., C. Rosa, C. Marzachì, M. Turina, and S. Palmano. "Detection of Flavescence Dorée Phytoplasma in Grapevine by Reverse-Transcription PCR." Plant Disease 91, no. 11 (November 2007): 1496–501. http://dx.doi.org/10.1094/pdis-91-11-1496.
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Flavescence dorée (FD) is the most serious phytoplasma disease of grapevine. This report describes a novel method of detecting FD phytoplasma based on reverse-transcription polymerase chain reaction (RT-PCR) on 16S ribosomal RNA (16SrRNA) which will greatly improve mass screening of infected grapevines. A rapid protocol for extracting sap from whole leaves or midveins and successive one-tube amplification by RT-PCR was applied to grapevine samples with or without symptoms collected from different areas of Piedmont (northwestern Italy). Results were compared with those obtained using one of the current diagnostic methods that utilizes nested PCR on phytoplasma DNA-enriched preparations. A Cohen's kappa index of 0.76 indicated a substantial agreement between the two sets of results. The RT-PCR method has the advantage of being a rapid, reliable, and sensitive assay for large-scale screening of grapevines.
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Campayo, Ana, Kortes Serrano de la Hoz, M. Mercedes García-Martínez, M. Rosario Salinas, and Gonzalo L. Alonso. "Spraying Ozonated Water on Bobal Grapevines: Effect on Wine Quality." Biomolecules 10, no. 2 (February 1, 2020): 213. http://dx.doi.org/10.3390/biom10020213.
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Ozonated water is being introduced as an alternative phytosanitary treatment to control grapevine diseases in a context in which the reduction of chemical pesticides has become an urgent necessity. In this study, we evaluated the effect of spraying grapevines with ozonated water on the enological, phenolic, and aromatic qualities of Bobal wines during two consecutive growing seasons. In the first season, ozonated water was applied once during the ripening period on grapevines trained on the traditional gobelet system (S1). In the second season, three applications were performed between fruit set and harvest on grapevines grown on a vertical trellis system (S2). The S1 treatment led to a wine with an increased alcoholic degree and a remarkably higher phenolic content, which resulted in preferable chromatic characteristics. The S2 treatment maintained the total phenolic content but significantly enhanced stilbenes and flavanols and also reduced anthocyanins, which negatively affected the wine colour. Regarding aroma, both treatments reduced the content of glycosylated precursors and had different effects on free volatiles, both varietal and fermentative. Thus, the metabolic response of grapevines to the ozonated water stress, and therefore the quality of wines, depended on the ozone dose received by the plants.
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Jagunić, Martin, Alfredo Diaz-Lara, Lóránt Szőke, Maher Al Rwahnih, Kristian Stevens, Goran Zdunić, and Darko Vončina. "Incidence and Genetic Diversity of Grapevine Virus G in Croatian Vineyards." Plants 11, no. 18 (September 7, 2022): 2341. http://dx.doi.org/10.3390/plants11182341.
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Grapevine virus G (GVG) is a recently discovered vitivirus infecting grapevines. Historically, viruses in the genus Vitivirus have been associated with the grapevine rugose wood disease. Based on new and previously reported GVG isolates, primers and probes were developed for real-time RT-PCR. The developed assay successfully detected the virus in infected plants during dormancy and the growing season. A field study of 4327 grapevines from Croatian continental and coastal wine-growing regions confirmed the presence of GVG in 456 (~10.5%) grapevines from three collection plantations and 77 commercial vineyards, with infection rates ranging from 2% to 100%. Interestingly, the virus was confirmed only in vines considered to be Croatian autochthonous cultivars, but not in introduced cultivars. A 564-nucleotide long portion of the coat protein gene from previously known and newly characterized GVG isolates had nucleotide and amino acid identities ranging from 89% to 100% and from 96.8% to 100%, respectively. Phylogenetic analysis revealed five distinct groups, with isolates originating from the same site being close to each other, indicating possible local infection. The information presented in this manuscript sets the stage for future studies to better understand the ecology and epidemiology of GVG and the possible need for inclusion in certification schemes.