Journal articles on the topic 'Grand Trunk Road (India)'

The essay calls for a re-imagining and reshaping of colonial constructs. It concisely encapsulates the history of the Grand Trunk Road (GT Road), from the 16th century when it was referred to as ‘Sadak-e-Azam’ to the late 19th century, when the road was completed under the administration of Lord William Bentinck and was renamed as ‘The Grand Trunk Road’ to contemporary times when it connects multiple cities with National Highways as part of the Golden Quadrilateral project and remains a ‘continuum’ that covers a distance of over 2,500 kilometres. While highlighting its importance in terms of its criticality as a geopolitical/strategic connect, the essay concludes on the note that there is much more to the GT Road than being a mere logistical, infrastructural tool. It serves as a political and cultural connect as well as embodies a way of life and these historic and organic connections require reinforcement. The essay underlines the symbolic value of the GT Road, while it comprises the mainstay of commerce in the subcontinent but, at the same time is significant in terms of rearranging social and political hierarchies, in other words, it constitutes an intrinsic part of the broader narrative of the south Asian space.

The geometric design of roads is the branch of highway engineering concerned with the positioning of the physical elements of the roadway according to standards and limitations with objectives to optimize efficiency and safety while minimizing cost and environmental damage. The present study aims to explore geometric design and other factors which cause of accidents in Lahore. Data is collected from TEPA (Traffic Engineering and Planning Agency), NESPAK (National Engineering Services Pakistan), CTP (City Traffic Police) and Rescue 1122 over a period of 3 years. Two phase sampling technique has been used. Data is carried out about demographic information, physical characteristics and geometric design of roads. All registered 356 traffic accidents have been used on Ferozpur Road, Multan Road, Canal Bank Road and Grand Trunk in Lahore. Poisson regression and negative binomial regression are discussed in this research. SPSS and R Language are used for analysis. The results show that most of accidents occur at office off timing and fatal due to reckless driving and over speeding. Mostly, cars and tralala hit the bikes and Pedestrians. The Poisson regression model gives good description of number of accidents depends on various explanatory variables. Number of lanes, type of locations and roadway light are statistically significant. Narrow Shoulder width (m), Median Width (m) and Lane width (m) increase accident occurrence. Three lanes and larger road structures increase accidents. Numbers of accident increase when Roadway, type of locations, roadway light and traffic control signals decrease.

China’s ‘One Belt, One Road’ (OBOR) initiative is a grand plan to connect Asia, Europe and East Africa economically. However, from India’s point of view, China’s attempt at what it describes as geo-economic expansion appears as a geopolitical threat because of China’s activity in the Indian Ocean Region (IOR). This essay assesses the extent to which OBOR is (i) realistic and achievable, (ii) a genuine threat to India and (iii) amenable to possible Indian involvement. It concludes that India should continue to be wary of its neighbour in national security terms, but has no option but to cooperate with China economically by participating in OBOR if it seriously wishes to increase economic growth and bring the ‘Make in India’ and Sagarmala projects to fruition. In particular, India should seriously consider allowing Chinese companies to develop one or more Indian ports with associated infrastructure and manufacturing.

Abstract. Since the Tang Dynasty (618–907 AD), the Tang-Tibet Road has been the only way from inland China to Qinghai and Tibet, and even to other countries such as Nepal and India. It ties and bonds various ethnic groups and regions, integrates cultural memories and cross-cultural communication achievements from ancient times to the present, and witnesses the dynamic propagation of the culture. Affected by the environment, climate, and wars, Tang-Tibet Road was often impossible to travel on or through intermittently during its progressive development in history. Routes and lines of each of its sections changed from time to time; eventually, an ancient road network was formed, consisting of one trunk road, two subsidiary roads in the north and south, several branches, and scattered auxiliary routes separated from the system, among which there were both outward-oriented international passages and inward-oriented passages. Nonetheless, research on Tang-Tibet Road is insufficient at the current stage. Regarding the problems summarized based on the review of the research situation, the present work probes deeper into the network structure of Tang-Tibet Road. How historical corridor is generated and evolved is understood from a regional perspective. In particular, strategies to design a space information system for the Tibet section of Tang-Tibet Road are explained to promote the exploration and use of cultural heritages in Tibet, in an effort to preserve these heritages while developing Tibet’s society and economy.

China’s Belt and Road Initiative (BRI) is the grand strategy that interlinks the regions in the common chain of trade and economy. The paper investigates whether China’s rise through this initiative is peaceful or whether the claims and worries of the U.S., India and Japan are true that it is rising as a hegemonic and aggressive country. The descriptive-analytical method is being used in this research work. China is growing, particularly through this global interconnectivity initiative. Its economic and political influence is greatly increasing. It connects the countries in an initiative that boosts their economic and social development and peace. It is an initiative of equal benefits and considerably growing interdependence among the states and non-state actors. It constructs a world that has common interests and common security. The new trend growing between China, U.S., India, and Japan consists of competition and friendly cooperative relations. China’s main priority is bringing peace, development, and a better standard of life to its people first. It does not want to repeat the history when its people were smashed badly during the opium war of 1840 and Japan’s occupation in World War II. It does learn the value of peace from its past.

This article challenges scholarly understanding of an 1837 mutiny in the First West India Regiment. In the Anglo-Trinidadian narrative, African-born soldiers acted out of blind rage, failing in their rebellion because they lacked skill with rifles and bayonets and did not understand either the terrain of Trinidad or its location in the Atlantic littoral. This article’s counterargument is that the rebels, led by a former slave-trader, Dâaga, who had been kidnaped by Portuguese traders at either Grand-Popo or Little Popo, was, with other African-born soldiers, well familiar with military weapons and, after time in the Caribbean, the ecosystem, society, and topography of Trinidad. Dâaga aimed at escape from eastern Trinidad for either Tobago or nearby South America, but was thwarted after English officers captured some mutineers, while the soldiers who remained on the run clashed with a mixed-race Spanish-speaking militia on the only road to an east-coast point of escape.

Princely building ventures in post 1857 colonial India included, among others, construction of religious buildings, even as their patrons enthusiastically pursued the colonial modernist agenda. This paper examines the architectural patronage of the Bhopal Begums, the women rulers of Bhopal State, who raised three grand mosques in their capital, Bhopal, in the 19th and early 20th century. As Bhopal marched on the road to progress under the Begums’ patronage, the mosques heralded the presence of Islam in the city in the post uprising scenario where both Muslims and mosques were subjected to retribution for fomenting the 1857 insurrection. Bhopal’s mosques were not only sacred sites for the devout but also impacted the public realm of the city. Their construction drew significantly on the Mughal architectural archetype, thus affording the Begums an opportunity to assert themselves, via their mosques, as legitimate inheritors of the Mughal legacy, including taking charge of the latter’s legacy of stewardship of Iam. Today, the Bhopal mosques constitute an integral part of the city’s built heritage corpus. It is worth underscoring that they are not only important symbols of the Muslim faith but also markers of their patrons’ endeavour to position themselves at the forefront in the complex political and cultural scenario of post uprising colonial India.

For the past decade, China and Japan have been competing against each other over aid market with its implicit intention to pursue their economic interests, which turned into a rivalry between two diplomatic concepts: Chinese Belt and Road Initiative (BRI) versus Japanese Free and Open Indo-Pacific (FOIP) Strategy. The severe competition over high-speed rail (HSR) by two countries, joined by French and German competitors, has been intensified for the benefit of China, taking all of its catchup benefits with its dubious sense of rule of law. This article asks if the two initiatives can coexist with the entry of India into the game and form an equilibrium of ‘grand coalition’, benefiting all its players as ‘win-win-win’ game, which turns out to be feasible and unstable. Nevertheless, Japan still seems to have a good reason to keep India as a strategic partner of FOIP as well as a ‘gateway’ for Central and Western Asia with fragile states such as Afghanistan and Pakistan, and official development assistance (ODA) could play a significant catalyst role as ‘human security’ promoted by Professor Amartya Sen and ex-UNHCR Ms. Sadako Ogata. The COVID-19 pandemic together with the exit of the isolationist US administration may provide the world with glimmering sense of hope for the year 2021 and beyond, if and only if one becomes more aware of the complementarities of comparative advantage of China, India and Japan to pursue common interests.

Twenty five years after the dissolution of the former Soviet Union and the reemergence of the Central Asian Republics as independent States, this region continues to be the scene of rivalries amongst the big powers (Russia, China, the European Union, USA, India, Japan, Turkey and Iran) competing in a sort of a “new Grand Game” to increase their geopolitical and economic influence in the region. Its geography, diverse and rich natural resources, like oil and gas, explain the ongoing hidden rivalries. Despite its common historical and cultural past, Central Asia constitutes a heterogeneous region with a multiethnic and multi-linguistic composition and a low degree of physical, economic and trade integration. The lack of mutual trust, the persisting tension over borders and the use and sharing of natural resources, as well as different levels of economic performance have so far jeopardized the development of genuine regional cooperation. Against this background, the idea behind the Chinese OBOR Initiative to develop rapid transportation, if well implemented, could theoretically, by improving interregional connectivity, develop the Central Asia regional market and foster intergovernmental cooperation and people-to-people contacts within the region. In this context, could we expect that the ambitious Chinese OBOR Initiative will boost geopolitical stability and promote shared economic and trade benefits in Central Asia? What are the conditions for that need to be fulfilled?The author analyzes in depth certain crucial political, economic and institutional requirements for the successful implementation of the OBOR Strategy and concludes, however, that three years after the launching of this crucial instrument of the Chinese “globalization without democracy” model, its implementation faces major problems and thus raises more doubts than certainties.

AbstractThe study was undertaken in South Andaman district, comprising three tehsils, viz. Port Blair, Ferrargunj and Little Andaman Tehsils, respectively. Intensive pupal infestation surveys were carried out along the National Highway (NH 223), the main passenger and trade route, referred to as Great Andaman Trunk Road. Sampling locations at every 3 km were geo-referenced with global positioning system unit. A total of 17314 water collections were examined from 29 locations across the South Andaman district, among which 1021 (5.9%) were colonized by immature stages of Aedes albopictus, Aedes aegypti and other mosquito species. Ae. aegypti were found in 12 locations, showing higher infestation in the densely built Aberdeen Bazaar. Breeding populations of Ae. albopictus were observed in 27 sampling locations. Both the species were not recorded in two Northern localities. In the areas where both the species are present, they were often found in the same developmental sites, suggesting convergent habitat selection. The most frequently encountered man-made, artificial and natural developmental sites were fixed cement tanks, plastic drums, plastic cans, metal drums, metal pots, discarded tires, coconut shells, leaf axils and tree holes. Ae. aegypti and Ae. albopictus were observed in varying proportions in Port Blair and Ferrargunj Tehsils, while the former species appeared to be absent in Little Andaman. This study elucidates the spatial distribution of Ae. aegypti and Ae. albopictus with preponderance of the latter species, pointing towards arboviral transmission and assumes public health importance in South Andaman district, endemic for dengue.

The Chinese model of development is a grand global strategy for achieving the Chinese dream through existing foreign policy. China - Sri Lanka bilateral relations have proved to be of increasingly prominent regional and global significance. The paper tries to examine the state of the China - Sri Lanka economic relationship in the context of the Chinese model of development. It is a descriptive study based on secondary data gathered from various sources of information. The Chinese model of development holds much appeal to many developing countries. However, this model is facing serious challenges and has become the subject of much debate in the recent past. The China - Sri Lanka relations have existed more than two thousand years because of the Island of Sri Lanka's strategic location on the old shipping lane. Sri Lanka is still considered a vital part of the String of Pearls concept. Hence, China has turned its attention towards Sri Lanka. From the beginning of the 21st century, Sri Lanka and China ratified several agreements on bilateral issues relating to the Belt and Road Initiative and Silk Road project. The recent developments in the China - Sri Lanka relationship have led to the creation of a so-called Chinese Colony in the South Asian region, and it has triggered China - India geopolitical interests in the Indian Ocean region. This paper concludes with the argument that the Sri Lankan Government will have to be receptive and sensitive on these issues, and pay attention to controlling contamination and maintaining diplomatic relations with all for the political stability and economic prosperity of the nation.

Abstract The post-Cold War international system, dominated by the United States, has been shaken by the relative downturn of the US economy and the simultaneous rise of China. China is rapidly emerging as a serious contender for America’s dominance of the Indo-Pacific. What is noticeable is the absence of intense balance of power politics in the form of formal military alliances among the states in the region, unlike state behaviour during the Cold War era. Countries are still hedging as their strategic responses towards each other evolve. We argue that the key factor explaining the absence of intense hard balancing is the dearth of existential threat that either China or its potential adversaries feel up till now. The presence of two related critical factors largely precludes existential threats, and thus hard balancing military coalitions formed by or against China. The first is the deepened economic interdependence China has built with the potential balancers, in particular, the United States, Japan, and India, in the globalisation era. The second is the grand strategy of China, in particular, the peaceful rise/development, and infrastructure-oriented Belt and Road Initiative. Any radical changes in these two conditions leading to existential threats by the key states could propel the emergence of hard-balancing coalitions.

China-Pakistan Economic Corridor (CPEC) was launched in 2015 when 51 agreements and MoUs (Memorandums of Understanding) worth USD 46 billion were signed between China and Pakistan. This grand-scale bilateral project is a flagship constituent of China’s Belt and Road Initiative (BRI). CPEC started with the goal of modernizing Pakistan’s road, rail, air, and energy transportation systems and enhancing connectivity between its Gwadar and Karachi ports to the Chinese province of Xinjiang and beyond. For Pakistan, CPEC not only meant an opportunity to address its existing challenges like the energy crisis but also a golden moment to emerge as a hub for economic activities in the region. This article discusses comparatives in Geo-Strategic countries, which can suggest the future course of CPEC projects. India, Russia, and Iran have agreed in 2000 to construct a route between Mumbai and St-Petersburg through Tehran and Moscow to facilitate the movement of energy resources from Central Asia. Likewise, China-Pakistan Corridor will increase economic activity between both countries and the region. This Corridor may also be beneficial to “String of Pearls” that China’s “culture” to save its sea lives of communication catering to protect oil and gas supplies, which are emerging from the Gulf of Persia, Middle East, and China. Investors will take advantage of various economic-related activities to promote the agriculture industry, tourism, construction activities, vehicle industry, information technology, etc. Corridors will fulfil the energy resource needs of many countries through the Caspian Sea and Central Asian Republics. However, this project is facing internal and external threats, Pakistan is facing terrorist attacks, and the Chinese province Xinjiang is facing threats. Serious security threats to this project are likely to emanate from Pakistan, Afghanistan, and Iran. The Uighur terrorists have a close relationship with the Taliban in FATA, which is a serious and adverse threat to Chinese interests in Pakistan. This thesis is an effort to find reasons and suggest suitable measures for the Government to sort out challenges to avoid delays in the implementation and completion of the project.

In the changing regional geo-political scenario China, Pakistan and other regional countries are set to play a vital role in the emerging regional economic corridor. China Pakistan Economic Corridor (CPEC) as part of “One Belt, One Road” grand strategy connect countries with China through economic collaboration extending from South East Asia to the Europe through maritime and land routes comprised of emerging markets of Africa and Middle East energy resources. This paper has investigated the significance of CPEC in parlance of theoretical framework of complex interdependence because the phenomenon of interdependence has tied state and non-state entities in economic and strategic dimensions. Regional connectivity results from systematic, upgraded and need-based interaction in perspective of CPEC for socio-economic, industrial, energy and trade development. CPEC is important not only for Pakistan and China but also for the other regional countries such as Afghanistan, Central Asian Republics, Iran and India. As international and regional politics follows interest’s game hence CPEC has also become matter of concerns for both regional and international players which has been amply illustrated by this research. These concerns are followed by arguments that CPEC may be seen in a broader perspective of interdependency with emphasis on strategic and economic dimensions guaranteeing regional connectivity and integration. CPEC can serve as a main trade avenue not only for China and Pakistan but for Central Asian Republics, Afghanistan, Iran and India as well. This study has argued that CPEChas the potential of becoming an energy transit corridor for growing economies. It has advantagesfor India also in form of improved infrastructure, communication and access to CARs market. Simultaneously it imparts an opportunity of flourishing and trade to the Afghanistan’s industries. Iran can also have benefit by ensuring Chabahar port outstretched to CPEC. CPEC has potential geopolitical risks as hostile environment of South Asia and the respective power rivalry and policy shift of world powers towards this region as manifested by the US pivot to Asia and the former concern over Chinese presence in the Arabian Sea in Asian region, Gulf states considering of Gwadar Port as rival port, Indian opposition of CPEC by arguing that it passes through Pakistan’s held Kashmir on which India has also claim. China and Pakistan in order to escape proxy tussle in Baluchistan need to settle Indian apprehension about the project, Confidence Building Measures (CBM) must be incorporated in order to promote closer ties with Afghanistan, Pakistan must attempt for having stable relations with Iran in order to overcome impediments to the CPEC success. By making CPEC a reality the benefits will be reaped by whole region and the very enmity of India with Pakistan and China can be turned into mutual beneficial economic relations. If India becomes a partner in the project then certainly the concerns of India will be settled down.

Environments containing significant concentration of NaCl such as salt lakes harbor extremophiles microorganisms which have a great biotechnology interest. To explore the diversity of Bacteria in Chott Tinsilt (Algeria), an isolation program was performed. Water samples were collected from the saltern during the pre-salt harvesting phase. This Chott is high in salt (22.47% (w/v). Seven halophiles Bacteria were selected for further characterization. The isolated strains were able to grow optimally in media with 10–25% (w/v) total salts. Molecular identification of the isolates was performed by sequencing the 16S rRNA gene. It showed that these cultured isolates included members belonging to the Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus genera with less than 98% of similarity with their closest phylogenetic relative. The halophilic bacterial isolates were also characterized for the production of biosurfactant and industrially important enzymes. Most isolates produced hydrolases and biosurfactants at high salt concentration. In fact, this is the first report on bacterial strains (A4 and B4) which were a good biosurfactant and coagulase producer at 20% and 25% ((w/v)) NaCl. In addition, the biosurfactant produced by the strain B4 at high salinity (25%) was also stable at high temperature (30-100°C) and high alkalinity (pH 11).Key word: Salt Lake, Bacteria, biosurfactant, Chott, halophiles, hydrolases, 16S rRNAINTRODUCTIONSaline lakes cover approximately 10% of the Earth’s surface area. The microbial populations of many hypersaline environments have already been studied in different geographical regions such as Great Salt Lake (USA), Dead Sea (Israel), Wadi Natrun Lake (Egypt), Lake Magadi (Kenya), Soda Lake (Antarctica) and Big Soda Lake and Mono Lake (California). Hypersaline regions differ from each other in terms of geographical location, salt concentration and chemical composition, which determine the nature of inhabitant microorganisms (Gupta et al., 2015). Then low taxonomic diversity is common to all these saline environments (Oren et al., 1993). Halophiles are found in nearly all major microbial clades, including prokaryotic (Bacteria and Archaea) and eukaryotic forms (DasSarma and Arora, 2001). They are classified as slight halophiles when they grow optimally at 0.2–0.85 M (2–5%) NaCl, as moderate halophiles when they grow at 0.85–3.4 M (5–20%) NaCl, and as extreme halophiles when they grow at 3.4–5.1 M (20–30%) NaCl. Hyper saline environments are inhabited by extremely halophilic and halotolerant microorganisms such as Halobacillus sp, Halobacterium sp., Haloarcula sp., Salinibacter ruber , Haloferax sp and Bacillus spp. (Solomon and Viswalingam, 2013). There is a tremendous demand for halophilic bacteria due to their biotechnological importance as sources of halophilic enzymes. Enzymes derived from halophiles are endowed with unique structural features and catalytic power to sustain the metabolic and physiological processes under high salt conditions. Some of these enzymes have been reported to be active and stable under more than one extreme condition (Karan and Khare, 2010). Applications are being considered in a range of industries such as food processing, washing, biosynthetic processes and environmental bioremediation. Halophilic proteases are widely used in the detergent and food industries (DasSarma and Arora, 2001). However, esterases and lipases have also been useful in laundry detergents for the removal of oil stains and are widely used as biocatalysts because of their ability to produce pure compounds. Likewise, amylases are used industrially in the first step of the production of high fructose corn syrup (hydrolysis of corn starch). They are also used in the textile industry in the de-sizing process and added to laundry detergents. Furthermore, for the environmental applications, the use of halophiles for bioremediation and biodegradation of various materials from industrial effluents to soil contaminants and accidental spills are being widely explored. In addition to enzymes, halophilic / halotolerants microorganisms living in saline environments, offer another potential applications in various fields of biotechnology like the production of biosurfactant. Biosurfactants are amphiphilic compounds synthesized from plants and microorganisms. They reduce surface tension and interfacial tension between individual molecules at the surface and interface respectively (Akbari et al., 2018). Comparing to the chemical surfactant, biosurfactant are promising alternative molecules due to their low toxicity, high biodegradability, environmental capability, mild production conditions, lower critical micelle concentration, higher selectivity, availability of resources and ability to function in wide ranges of pH, temperature and salinity (Rocha et al., 1992). They are used in various industries which include pharmaceuticals, petroleum, food, detergents, cosmetics, paints, paper products and water treatment (Akbari et al., 2018). The search for biosurfactants in extremophiles is particularly promising since these biomolecules can adapt and be stable in the harsh environments in which they are to be applied in biotechnology.OBJECTIVESEastern Algeria features numerous ecosystems including hypersaline environments, which are an important source of salt for food. The microbial diversity in Chott Tinsilt, a shallow Salt Lake with more than 200g/L salt concentration and a superficies of 2.154 Ha, has never yet been studied. The purpose of this research was to chemically analyse water samples collected from the Chott, isolate novel extremely or moderate halophilic Bacteria, and examine their phenotypic and phylogenetic characteristics with a view to screening for biosurfactants and enzymes of industrial interest.MATERIALS AND METHODSStudy area: The area is at 5 km of the Commune of Souk-Naâmane and 17 km in the South of the town of Aïn-Melila. This area skirts the trunk road 3 serving Constantine and Batna and the railway Constantine-Biskra. It is part the administrative jurisdiction of the Wilaya of Oum El Bouaghi. The Chott belongs to the wetlands of the High Plains of Constantine with a depth varying rather regularly without never exceeding 0.5 meter. Its length extends on 4 km with a width of 2.5 km (figure 1).Water samples and physico-chemical analysis: In February 2013, water samples were collected from various places at the Chott Tinsilt using Global Positioning System (GPS) coordinates of 35°53’14” N lat. and 06°28’44”E long. Samples were collected randomly in sterile polythene bags and transported immediately to the laboratory for isolation of halophilic microorganisms. All samples were treated within 24 h after collection. Temperature, pH and salinity were measured in situ using a multi-parameter probe (Hanna Instruments, Smithfield, RI, USA). The analytical methods used in this study to measure ions concentration (Ca2+, Mg2+, Fe2+, Na+, K+, Cl−, HCO3−, SO42−) were based on 4500-S-2 F standard methods described elsewhere (Association et al., 1920).Isolation of halophilic bacteria from water sample: The media (M1) used in the present study contain (g/L): 2.0 g of KCl, 100.0/200.0 g of NaCl, 1.0 g of MgSO4.7HO2, 3.0 g of Sodium Citrate, 0.36 g of MnCl2, 10.0 g of yeast extract and 15.0 g agar. The pH was adjusted to 8.0. Different dilutions of water samples were added to the above medium and incubated at 30°C during 2–7 days or more depending on growth. Appearance and growth of halophilic bacteria were monitored regularly. The growth was diluted 10 times and plated on complete medium agar (g/L): glucose 10.0; peptone 5.0; yeast extract 5.0; KH2PO4 5.0; agar 30.0; and NaCl 100.0/200.0. Resultant colonies were purified by repeated streaking on complete media agar. The pure cultures were preserved in 20% glycerol vials and stored at −80°C for long-term preservation.Biochemical characterisation of halophilic bacterial isolates: Bacterial isolates were studied for Gram’s reaction, cell morphology and pigmentation. Enzymatic assays (catalase, oxidase, nitrate reductase and urease), and assays for fermentation of lactose and mannitol were done as described by Smibert (1994).Optimization of growth conditions: Temperature, pH, and salt concentration were optimized for the growth of halophilic bacterial isolates. These growth parameters were studied quantitatively by growing the bacterial isolates in M1 medium with shaking at 200 rpm and measuring the cell density at 600 nm after 8 days of incubation. To study the effect of NaCl on the growth, bacterial isolates were inoculated on M1 medium supplemented with different concentration of NaCl: 1%-35% (w/v). The effect of pH on the growth of halophilic bacterial strains was studied by inoculating isolates on above described growth media containing NaCl and adjusted to acidic pH of 5 and 6 by using 1N HCl and alkaline pH of 8, 9, 10, 11 and 12 using 5N NaOH. The effect of temperature was studied by culturing the bacterial isolates in M1 medium at different temperatures of incubation (4°C–55°C).Screening of halophilic bacteria for hydrolytic enzymes: Hydrolase producing bacteria among the isolates were screened by plate assay on starch, tributyrin, gelatin and DNA agar plates respectively for amylase, lipase, protease and DNAse activities. Amylolytic activity of the cultures was screened on starch nutrient agar plates containing g/L: starch 10.0; peptone 5.0; yeast extract 3.0; agar 30.0; NaCl 100.0/250.0. The pH was 7.0. After incubation at 30 ºC for 7 days, the zone of clearance was determined by flooding the plates with iodine solution. The potential amylase producers were selected based on ratio of zone of clearance diameter to colony diameter. Lipase activity of the cultures was screened on tributyrin nutrient agar plates containing 1% (v/v) of tributyrin. Isolates that showed clear zones of tributyrin hydrolysis were identified as lipase producing bacteria. Proteolytic activity of the isolates was similarly screened on gelatin nutrient agar plates containing 10.0 g/L of gelatin. The isolates showing zones of gelatin clearance upon treatment with acidic mercuric chloride were selected and designated as protease producing bacteria. The presence of DNAse activity on plates was determined on DNAse test agar (BBL) containing 10%-25% (w/v) total salt. After incubation for 7days, the plates were flooded with 1N HCl solution. Clear halos around the colonies indicated DNAse activity (Jeffries et al., 1957).Milk clotting activity (coagulase activity) of the isolates was also determined following the procedure described (Berridge, 1952). Skim milk powder was reconstituted in 10 mM aqueous CaCl2 (pH 6.5) to a final concentration of 0.12 kg/L. Enzyme extracts were added at a rate of 0.1 mL per mL of milk. The coagulation point was determined by manual rotating of the test tube periodically, at short time intervals, and checking for visible clot formation.Screening of halophilic bacteria for biosurfactant production. Oil spread Assay: The Petridis base was filled with 50 mL of distilled water. On the water surface, 20μL of diesel and 10μl of culture were added respectively. The culture was introduced at different spots on the diesel, which is coated on the water surface. The occurrence of a clear zone was an indicator of positive result (Morikawa et al., 2000). The diameter of the oil expelling circles was measured by slide caliber (with a degree of accuracy of 0.02 mm).Surface tension and emulsification index (E24): Isolates were cultivated at 30 °C for 7 days on the enrichment medium containing 10-25% NaCl and diesel oil as the sole carbon source. The medium was centrifuged (7000 rpm for 20 min) and the surface tension of the cell-free culture broth was measured with a TS90000 surface tensiometer (Nima, Coventry, England) as a qualitative indicator of biosurfactant production. The culture broth was collected with a Pasteur pipette to remove the non-emulsified hydrocarbons. The emulsifying capacity was evaluated by an emulsification index (E24). The E24 of culture samples was determined by adding 2 mL of diesel oil to the same amount of culture, mixed for 2 min with a vortex, and allowed to stand for 24 h. E24 index is defined as the percentage of height of emulsified layer (mm) divided by the total height of the liquid column (mm).Biosurfactant stability studies : After growth on diesel oil as sole source of carbone, cultures supernatant obtained after centrifugation at 6,000 rpm for 15 min were considered as the source of crude biosurfactant. Its stability was determined by subjecting the culture supernatant to various temperature ranges (30, 40, 50, 60, 70, 80 and 100 °C) for 30 min then cooled to room temperature. Similarly, the effect of different pH (2–11) on the activity of the biosurfactant was tested. The activity of the biosurfactant was investigated by measuring the emulsification index (El-Sersy, 2012).Molecular identification of potential strains. DNA extraction and PCR amplification of 16S rDNA: Total cellular DNA was extracted from strains and purified as described by Sambrook et al. (1989). DNA was purified using Geneclean® Turbo (Q-BIO gene, Carlsbad, CA, USA) before use as a template in polymerase chain reaction (PCR) amplification. For the 16S rDNA gene sequence, the purified DNA was amplified using a universal primer set, forward primer (27f; 5′-AGA GTT TGA TCM TGG CTC AG) and a reverse primer (1492r; 5′-TAC GGY TAC CTT GTT ACG ACT T) (Lane, 1991). Agarose gel electrophoresis confirmed the amplification product as a 1400-bp DNA fragment.16S rDNA sequencing and Phylogenic analysis: Amplicons generated using primer pair 27f-1492r was sequenced using an automatic sequencer system at Macrogene Company (Seoul, Korea). The sequences were compared with those of the NCBI BLAST GenBank nucleotide sequence databases. Phylogenetic trees were constructed by the neighbor-joining method using MEGA version 5.05 software (Tamura et al., 2011). Bootstrap resembling analysis for 1,000 replicates was performed to estimate the confidence of tree topologies.Nucleotide sequence accession numbers: The nucleotide sequences reported in this work have been deposited in the EMBL Nucleotide Sequence Database. The accession numbers are represented in table 5.Statistics: All experiments were conducted in triplicates. Results were evaluated for statistical significance using ANOVA.RESULTSPhysico-chemical parameters of the collected water samples: The physicochemical properties of the collected water samples are reported in table 1. At the time of sampling, the temperature was 10.6°C and pH 7.89. The salinity of the sample, as determined in situ, was 224.70 g/L (22,47% (w/v)). Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions (table 1). SO4-2 and Mg+2 was present in much smaller amounts compared to Na +and Cl- concentration. Low levels of calcium, potassium and bicarbonate were also detected, often at less than 1 g/L.Characterization of isolates. Morphological and biochemical characteristic feature of halophilic bacterial isolates: Among 52 strains isolated from water of Chott Tinsilt, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for further characterization (table 2). The colour of the isolates varied from beige, pale yellow, yellowish and orange. The bacterial isolates A1, A2, A4, B1 and B5 were rod shaped and gram negative (except B5), whereas A3 and B4 were cocci and gram positive. All strains were oxidase and catalase positive except for B1. Nitrate reductase and urease activities were observed in all the bacterial isolates, except B4. All the bacterial isolates were negative for H2S formation. B5 was the only strain positive for mannitol fermentation (table 2).We isolated halophilic bacteria on growth medium with NaCl supplementation at pH 7 and temperature of 30°C. We studied the effect of NaCl, temperature and pH on the growth of bacterial isolates. All the isolates exhibited growth only in the presence of NaCl indicating that these strains are halophilic. The optimum growth of isolates A3 and B1 was observed in the presence of 10% NaCl, whereas it was 15% NaCl for A1, A2 and B5. A4 and B4 showed optimum growth in the presence of 20% and 25% NaCl respectively. A4, B4 and B5 strains can tolerate up to 35% NaCl.The isolate B1 showed growth in medium supplemented with 10% NaCl and pH range of 7–10. The optimum pH for the growth B1 was 9 and they did not show any detectable growth at or below pH 6 (table 2), which indicates the alkaliphilic nature of B1 isolate. The bacterial isolates A1, A2 and A4 exhibited growth in the range of pH 6–10, while A3 and B4 did not show any growth at pH greater than 8. The optimum pH for growth of all strains (except B1) was pH 7.0 (table 2). These results indicate that A1, A2, A3, A4, B4 and B5 are neutrophilic in nature. All the bacterial isolates exhibited optimal growth at 30°C and no detectable growth at 55°C. Also, detectable growth of isolates A1, A2 and A4 was observed at 4°C. However, none of the bacterial strains could grow below 4°C and above 50°C (table 2).Screening of the halophilic enzymes: To characterize the diversity of halophiles able to produce hydrolytic enzymes among the population of microorganisms inhabiting the hypersaline habitats of East Algeria (Chott Tinsilt), a screening was performed. As described in Materials and Methods, samples were plated on solid media containing 10%-25% (w/v) of total salts and different substrates for the detection of amylase, protease, lipase and DNAse activities. However, coagulase activity was determined in liquid medium using milk as substrate (figure 3). Distributions of hydrolytic activity among the isolates are summarized in table 4.From the seven bacterial isolates, four strains A1, A2, A4 and B5 showed combined hydrolytic activities. They were positive for gelatinase, lipase and coagulase. A3 strain showed gelatinase and lipase activities. DNAse activities were detected with A1, A4, B1 and B5 isolates. B4 presented lipase and coagulase activity. Surprisingly, no amylase activity was detected among all the isolates.Screening for biosurfactant producing isolates: Oil spread assay: The results showed that all the strains could produce notable (>4 cm diameter) oil expelling circles (ranging from 4.11 cm to 4.67 cm). The average diameter for strain B5 was 4.67 cm, significantly (P < 0.05) higher than for the other strains.Surface tension and emulsification index (E24): The assimilation of hydrocarbons as the sole sources of carbon by the isolate strains led to the production of biosurfactants indicated by the emulsification index and the lowering of the surface tension of cell-free supernatant. Based on rapid growth on media containing diesel oil as sole carbon source, the seven isolates were tested for biosurfactant production and emulsification activity. The obtained values of the surface tension measurements as well as the emulsification index (E24) are shown in table 3. The highest reduction of surface tension was achieved with B5 and A3 isolates with values of 25.3 mN m−1 and 28.1 mN m−1 respectively. The emulsifying capacity evaluated by the E24 emulsification index was highest in the culture of isolate B4 (78%), B5 (77%) and A3 (76%) as shown in table 3 and figure 2. These emulsions were stable even after 4 months. The bacteria with emulsification indices higher than 50 % and/or reduction in the surface tension (under 30 mN/m) have been defined as potential biosurfactant producers. Based on surface tension and the E24 index results, isolates B5, B4, A3 and A4 are the best candidates for biosurfactant production. It is important to note that, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was choosen for futher analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4.biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was chosen for further analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4. The biosurfactant produced by this strain was shown to be thermostable giving an E-24 Index value greater than 78% (figure 4A). Heating of the biosurfactant to 100 °C caused no significant effect on the biosurfactant performance. Therefore, the surface activity of the crude biosurfactant supernatant remained relatively stable to pH changes between pH 6 and 11. At pH 11, the value of E24 showed almost 76% activity, whereas below pH 6 the activity was decreased up to 40% (figure 4A). The decreases of the emulsification activity by decreasing the pH value from basic to an acidic region; may be due to partial precipitation of the biosurfactant. This result indicated that biosurfactant produced by strain B4 show higher stability at alkaline than in acidic conditions.Molecular identification and phylogenies of potential isolates: To identify halophilic bacterial isolates, the 16S rDNA gene was amplified using gene-specific primers. A PCR product of ≈ 1.3 kb was detected in all the seven isolates. The 16S rDNA amplicons of each bacterial isolate was sequenced on both strands using 27F and 1492R primers. The complete nucleotide sequence of 1336,1374, 1377,1313, 1305,1308 and 1273 bp sequences were obtained from A1, A2, A3, A4, B1, B4 and B5 isolates respectively, and subjected to BLAST analysis. The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus as shown in table 5. The halophilic isolates A2 and A4 showed 97% similarity with the Halomonas variabilis strain GSP3 (accession no. AY505527) and the Halomonas sp. M59 (accession no. AM229319), respectively. As for A1, it showed 96% similarity with the Halomonas venusta strain GSP24 (accession no. AY553074). B1 and B4 showed for their part 96% similarity with the Salinivibrio costicola subsp. alcaliphilus strain 18AG DSM4743 (accession no. NR_042255) and the Planococcus citreus (accession no. JX122551), respectively. The bacterial isolate B5 showed 98% sequence similarity with the Halobacillus trueperi (accession no. HG931926), As for A3, it showed only 95% similarity with the Staphylococcus arlettae (accession no. KR047785). The 16S rDNA nucleotide sequences of all the seven halophilic bacterial strains have been submitted to the NCBI GenBank database under the accession number presented in table 5. The phylogenetic association of the isolates is shown in figure 5.DICUSSIONThe physicochemical properties of the collected water samples indicated that this water was relatively neutral (pH 7.89) similar to the Dead Sea and the Great Salt Lake (USA) and in contrast to the more basic lakes such as Lake Wadi Natrun (Egypt) (pH 11) and El Golea Salt Lake (Algeria) (pH 9). The salinity of the sample was 224.70 g/L (22,47% (w/v). This range of salinity (20-30%) for Chott Tinsilt is comparable to a number of well characterized hypersaline ecosystems including both natural and man-made habitats, such as the Great Salt Lake (USA) and solar salterns of Puerto Rico. Thus, Chott Tinsilt is a hypersaline environment, i.e. environments with salt concentrations well above that of seawater. Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions, as in most hypersaline ecosystems (with some exceptions such as the Dead Sea). These chemical water characteristics were consistent with the previously reported data in other hypersaline ecosystems (DasSarma and Arora, 2001; Oren, 2002; Hacěne et al., 2004). Among 52 strains isolated from this Chott, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for phenotypique, genotypique and phylogenetique characterization.The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus. Genera obtained in the present study are commonly occurring in various saline habitats across the globe. Staphylococci have the ability to grow in a wide range of salt concentrations (Graham and Wilkinson, 1992; Morikawa et al., 2009; Roohi et al., 2014). For example, in Pakistan, Staphylococcus strains were isolated from various salt samples during the study conducted by Roohi et al. (2014) and these results agreed with previous reports. Halomonas, halophilic and/or halotolerant Gram-negative bacteria are typically found in saline environments (Kim et al., 2013). The presence of Planococcus and Halobacillus has been reported in studies about hypersaline lakes; like La Sal del Rey (USA) (Phillips et al., 2012) and Great Salt Lake (Spring et al., 1996), respectively. The Salinivibrio costicola was a representative model for studies on osmoregulatory and other physiological mechanisms of moderately halophilic bacteria (Oren, 2006).However, it is interesting to note that all strains shared less than 98.7% identity (the usual species cut-off proposed by Yarza et al. (2014) with their closest phylogenetic relative, suggesting that they could be considered as new species. Phenotypic, genetic and phylogenetic analyses have been suggested for the complete identification of these strains. Theses bacterial strains were tested for the production of industrially important enzymes (Amylase, protease, lipase, DNAse and coagulase). These isolates are good candidates as sources of novel enzymes with biotechnological potential as they can be used in different industrial processes at high salt concentration (up to 25% NaCl for B4). Prominent amylase, lipase, protease and DNAase activities have been reported from different hypersaline environments across the globe; e.g., Spain (Sánchez‐Porro et al., 2003), Iran (Rohban et al., 2009), Tunisia (Baati et al., 2010) and India (Gupta et al., 2016). However, to the best of our knowledge, the coagulase activity has never been detected in extreme halophilic bacteria. Isolation and characterization of crude enzymes (especially coagulase) to investigate their properties and stability are in progress.The finding of novel enzymes with optimal activities at various ranges of salt concentrations is of great importance. Besides being intrinsically stable and active at high salt concentrations, halophilic and halotolerant enzymes offer great opportunities in biotechnological applications, such as environmental bioremediation (marine, oilfiel) and food processing. The bacterial isolates were also characterized for production of biosurfactants by oil-spread assay, measurement of surface tension and emulsification index (E24). There are few reports on biosurfactant producers in hypersaline environments and in recent years, there has been a greater increase in interest and importance in halophilic bacteria for biomolecules (Donio et al., 2013; Sarafin et al., 2014). Halophiles, which have a unique lipid composition, may have an important role to play as surface-active agents. The archae bacterial ether-linked phytanyl membrane lipid of the extremely halophilic bacteria has been shown to have surfactant properties (Post and Collins, 1982). Yakimov et al. (1995) reported the production of biosurfactant by a halotolerant Bacillus licheniformis strain BAS 50 which was able to produce a lipopeptide surfactant when cultured at salinities up to 13% NaCl. From solar salt, Halomonas sp. BS4 and Kocuria marina BS-15 were found to be able to produce biosurfactant when cultured at salinities of 8% and 10% NaCl respectively (Donio et al., 2013; Sarafin et al., 2014). In the present work, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% NaCl. To our knowledge, this is the first report on biosurfactant production by bacteria under such salt concentration. Biosurfactants have a wide variety of industrial and environmental applications (Akbari et al., 2018) but their applicability depends on their stability at different environmental conditions. The strain B4 which can produce biosurfactant at 25% NaCl showed good stability in alkaline pH and at a temperature range of 30°C-100°C. Due to the enormous utilization of biosurfactant in detergent manufacture the choice of alkaline biosurfactant is researched (Elazzazy et al., 2015). On the other hand, the interesting finding was the thermostability of the produced biosurfactant even after heat treatment (100°C for 30 min) which suggests the use of this biosurfactant in industries where heating is of a paramount importance (Khopade et al., 2012). To date, more attention has been focused on biosurfactant producing bacteria under extreme conditions for industrial and commercial usefulness. In fact, the biosurfactant produce by strain B4 have promising usefulness in pharmaceutical, cosmetics and food industries and for bioremediation in marine environment and Microbial enhanced oil recovery (MEOR) where the salinity, temperature and pH are high.CONCLUSIONThis is the first study on the culturable halophilic bacteria community inhabiting Chott Tinsilt in Eastern Algeria. Different genera of halotolerant bacteria with different phylogeneticaly characteristics have been isolated from this Chott. Culturing of bacteria and their molecular analysis provides an opportunity to have a wide range of cultured microorganisms from extreme habitats like hypersaline environments. Enzymes produced by halophilic bacteria show interesting properties like their ability to remain functional in extreme conditions, such as high temperatures, wide range of pH, and high salt concentrations. 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Road infrastructure in good condition is a key requirement for efficient transportation systems which leads to economic prosperity and improved quality of life. However, road surface conditions deteriorate over time according to traffic loads and environmental factors. Poor road conditions lead to congestion, accidents, lost productivity, and driver fatigue. This work considers the relationship between road pavement condition and vehicle speed, vibration, and in-vehicle noise. A 7 km section of the Grand Trunk Road, Peshawar, Pakistan divided into 280 segments (140 for each lane), of length 50 m was observed and the Pavement Condition Index (PCI) of each segment was determined based on the recurrent distress type and density according to ASTM D6433-011 guidelines. The number of very good, satisfactory, fair, poor, and very poor conditions are 51, 52, 81, 48, and 42, respectively. The mobile app BotlnckDectr was employed to measure vehicle speed, RPM, noise, vibration, GPS location, and time. Statistical analysis was employed to determine the relationship between PCI and vehicle speed, vibration, and in-vehicle noise. The results obtained indicate that noise and vibration increase by 3.3% and more than 30%, respectively, as the pavement condition changes from good to very poor, and vehicle speed decreases by 8.8%.

This article primarily focuses on how the increasing US–China competing strategies in Asia-Pacific affect the policies of South Asian rivals India and Pakistan when, on the one hand, the US as part of its offshore balancing grand strategy has been increasing its strategic partnership with India through the transfer of emerging technologies in terms of military modernization process, and on the other hand, China and Pakistan have improved their geo-economic and geostrategic partnership as part of the Chinese grand strategy via the Belt and Road Initiative while enabling Pakistan to produce effective countermeasures against its potential adversary. The article presumes that, in doing so, such competing strategies frame a quadrangle setting comprising of US and India to deter and contain China on the one hand and China and Pakistan to produce countermeasures and try to create a balance to potentially prevent the risk of conflict in South Asia out of such competing strategies at the quadrangle order conceived here. However, in fact, neither the US nor rising China would desire such a possibility of conflict otherwise unintendedly occurring from the intense US–China competing strategies while affecting the policies of the South Asian rivals. The article concludes that the shaping of this quadrangle framework may bring both opportunities and challenges for the South Asian rivals. It also concludes that the more intense the competition between the US and China becomes, the more intense its implications could be on the South Asian rivals, while the reduced tension between China and the US, although unlikely, would have reduced pressure on India and Pakistan relations as well.

The area of Baranagore on the northern outskirts of Calcutta consists of various industries and factories interspersed with small neighbourhoods of middle-class homes. The main artery o f transport is the Barrackpore Trunk Road, a wide and straight thoroughfare originally built by the British Colonial rulers for the rapid movement of their troops from their barracks to the strategic sea-port of Calcutta. The Indian Statistical Institute occupies several city blocks along this highway. It was founded by Prasanta Chandra Mahalanobis, F.R.S., in the early 1930s as a small Statistical Laboratory at the Presidency College, Calcutta, where he was then the Professor o f Physics. At the time of Haldane’s arrival in India the institute employed 2000 workers. On 4 January 1958 I was ushered into a classroom on the second floor of the institute to await Haldane’s arrival. I had never met him before that date. I had read a great deal about his bad temper, ferocity and eccentric behaviour in the popular press. In response to my request for a research position under his direction, Haldane had earlier tested my knowledge in plant genetics with some assignments and was satisfied to the extent that he offered me a scholarship at the institute. In the mean time I was personally interviewed by Mahalanobis in New Delhi at Haldane’s request. As I was waiting for Haldane that morning I was far from certain that I had made the right decision to work with him.

Introduction The introduction of novel distal nerve transfers has changed the way in which brachial plexus surgery is being performed. Although full recovery of function after brachial plexus reconstruction still remains unachievable, the exciting developments of the past several years have considerably improved surgical outcomes, and future directions for research are promising. Aim And Ojective To review incidence, mechanics, various patterns, treatment regimes, outcome and morbidity factors of various types of Brachial plexus injury and various circumstances which led to Brachial plexus injury in our department. Material And Methods This study is prospective study and 24 patients with adult brachial plexus injury admitted during the period from 2014 to 2017 are included in this study. Patients with adult brachial plexus injury, initially treated elsewhere and referred to our department are also included in the study. Paediatric brachial plexus injury obstetric brachial plexus palsy are not included in this study Results In this study brachial plexus injury injuries are more common in male (90%) compared to female ( 20%), more in age group of 20-30 years( 54%), road trafc accident is the most common mode of injury ( 79.16%), maximum number of patients presented between 3 to 6 months of injury(50%), 75% patients had preganglionic injury and supraclavicular injury(95.8%) ,more common in right side(62.5%), upper and middle trunk mostly involved( 90% and 83.3% respectively). 79.16% patients were operated with anterior approach. Neurotization with spinal accessory nerve (SAN) to suprascapular nerve (SSN) done in 95% patients out of 24 patients through posterior approach. Neurotization with phrenic to musculocutaneous nerve (MC) and intercostal nerve (ICN) to musculocutaneous nerve through anterior approach in 79% and 21% patients respectively. Conclusion This study gives an insight into the epidemiological aspects of brachial plexus injuries in our unit which reects the situation in India and also about the few parameters of quality of life following brachial plexus injuries.

The curtain rose. The howling of desert wind filled the performance hall in the Shanghai Grand Theatre. Into the center stage, where a scenic construction of a mountain cliff and a desert landscape was dimly lit, entered the character of the Daoist priest Wang Yuanlu (1849–1931), performed by Chen Yizong. Dressed in a worn and dusty outfit of dark blue cotton, characteristic of Daoist priests, Wang began to sweep the floor. After a few moments, he discovered a hidden chambre sealed inside one of the rock sanctuaries carved into the cliff.Signaled by the quick, crystalline, stirring wave of sound from the chimes, a melodious Chinese ocarina solo joined in slowly from the background. Astonished by thousands of Buddhist sūtra scrolls, wall paintings, and sculptures he had just accidentally discovered in the caves, Priest Wang set his broom aside and began to examine these treasures. Dawn had not yet arrived, and the desert sky was pitch-black. Priest Wang held his oil lamp high, strode rhythmically in excitement, sat crossed-legged in a meditative pose, and unfolded a scroll. The sound of the ocarina became fuller and richer and the texture of the music more complex, as several other instruments joined in.Below is the opening scene of the award-winning, theatrical dance-drama Dunhuang, My Dreamland, created by China’s state-sponsored Lanzhou Song and Dance Theatre in 2000. Figure 1a: Poster Side A of Dunhuang, My Dreamland Figure 1b: Poster Side B of Dunhuang, My DreamlandThe scene locates the dance-drama in the rock sanctuaries that today are known as the Dunhuang Mogao Caves, housing Buddhist art accumulated over a period of a thousand years, one of the best well-known UNESCO heritages on the Silk Road. Historically a frontier metropolis, Dunhuang was a strategic site along the Silk Road in northwestern China, a crossroads of trade, and a locus for religious, cultural, and intellectual influences since the Han dynasty (206 B.C.E.–220 C.E.). Travellers, especially Buddhist monks from India and central Asia, passing through Dunhuang on their way to Chang’an (present day Xi’an), China’s ancient capital, would stop to meditate in the Mogao Caves and consult manuscripts in the monastery's library. At the same time, Chinese pilgrims would travel by foot from China through central Asia to Pakistan, India, Nepal, Bangladesh, and Sri Lanka, playing a key role in the exchanges between ancient China and the outside world. Travellers from China would stop to acquire provisions at Dunhuang before crossing the Gobi Desert to continue on their long journey abroad. Figure 2: Dunhuang Mogao CavesThis article approaches the idea of “abroad” by examining the present-day imagination of journeys along the Silk Road—specifically, staged performances of the various Silk Road journey-themed dance-dramas sponsored by the Chinese state for enhancing its cultural and foreign policies since the 1970s (Kuang).As ethnomusicologists have demonstrated, musicians, choreographers, and playwrights often utilise historical materials in their performances to construct connections between the past and the present (Bohlman; Herzfeld; Lam; Rees; Shelemay; Tuohy; Wade; Yung: Rawski; Watson). The ancient Silk Road, which linked the Mediterranean coast with central China and beyond, via oasis towns such as Samarkand, has long been associated with the concept of “journeying abroad.” Journeys to distant, foreign lands and encounters of unknown, mysterious cultures along the Silk Road have been documented in historical records, such as A Record of Buddhist Kingdoms (Faxian) and The Great Tang Records on the Western Regions (Xuanzang), and illustrated in classical literature, such as The Travels of Marco Polo (Polo) and the 16th century Chinese novel Journey to the West (Wu). These journeys—coming and going from multiple directions and to different destinations—have inspired contemporary staged performance for audiences around the globe.Home and Abroad: Dunhuang and the Silk RoadDunhuang, My Dreamland (2000), the contemporary dance-drama, staged the journey of a young pilgrim painter travelling from Chang’an to a land of the unfamiliar and beyond borders, in search for the arts that have inspired him. Figure 3: A scene from Dunhuang, My Dreamland showing the young pilgrim painter in the Gobi Desert on the ancient Silk RoadFar from his home, he ended his journey in Dunhuang, historically considered the northwestern periphery of China, well beyond Yangguan and Yumenguan, the bordering passes that separate China and foreign lands. Later scenes in Dunhuang, My Dreamland, portrayed through multiethnic music and dances, the dynamic interactions among merchants, cultural and religious envoys, warriors, and politicians that were making their own journey from abroad to China. The theatrical dance-drama presents a historically inspired, re-imagined vision of both “home” and “abroad” to its audiences as they watch the young painter travel along the Silk Road, across the Gobi Desert, arriving at his own ideal, artistic “homeland”, the Dunhuang Mogao Caves. Since his journey is ultimately a spiritual one, the conceptualisation of travelling “abroad” could also be perceived as “a journey home.”Staged more than four hundred times since it premiered in Beijing in April 2000, Dunhuang, My Dreamland is one of the top ten titles in China’s National Stage Project and one of the most successful theatrical dance-dramas ever produced in China. With revenue of more than thirty million renminbi (RMB), it ranks as the most profitable theatrical dance-drama ever produced in China, with a preproduction cost of six million RMB. The production team receives financial support from China’s Ministry of Culture for its “distinctive ethnic features,” and its “aim to promote traditional Chinese culture,” according to Xu Rong, an official in the Cultural Industry Department of the Ministry. Labeled an outstanding dance-drama of the Chinese nation, it aims to present domestic and international audiences with a vision of China as a historically multifaceted and cosmopolitan nation that has been in close contact with the outside world through the ancient Silk Road. Its production company has been on tour in selected cities throughout China and in countries abroad, including Austria, Spain, and France, literarily making the young pilgrim painter’s “journey along the Silk Road” a new journey abroad, off stage and in reality.Dunhuang, My Dreamland was not the first, nor is it the last, staged performances that portrays the Chinese re-imagination of “journeying abroad” along the ancient Silk Road. It was created as one of many versions of Dunhuang bihua yuewu, a genre of music, dance, and dramatic performances created in the early twentieth century and based primarily on artifacts excavated from the Mogao Caves (Kuang). “The Mogao Caves are the greatest repository of early Chinese art,” states Mimi Gates, who works to increase public awareness of the UNESCO site and raise funds toward its conservation. “Located on the Chinese end of the Silk Road, it also is the place where many cultures of the world intersected with one another, so you have Greek and Roman, Persian and Middle Eastern, Indian and Chinese cultures, all interacting. Given the nature of our world today, it is all very relevant” (Pollack). As an expressive art form, this genre has been thriving since the late 1970s contributing to the global imagination of China’s “Silk Road journeys abroad” long before Dunhuang, My Dreamland achieved its domestic and international fame. For instance, in 2004, The Thousand-Handed and Thousand-Eyed Avalokiteśvara—one of the most representative (and well-known) Dunhuang bihua yuewu programs—was staged as a part of the cultural program during the Paralympic Games in Athens, Greece. This performance, as well as other Dunhuang bihua yuewu dance programs was the perfect embodiment of a foreign religion that arrived in China from abroad and became Sinicized (Kuang). Figure 4: Mural from Dunhuang Mogao Cave No. 45A Brief History of Staging the Silk Road JourneysThe staging of the Silk Road journeys abroad began in the late 1970s. Historically, the Silk Road signifies a multiethnic, cosmopolitan frontier, which underwent incessant conflicts between Chinese sovereigns and nomadic peoples (as well as between other groups), but was strongly imbued with the customs and institutions of central China (Duan, Mair, Shi, Sima). In the twentieth century, when China was no longer an empire, but had become what the early 20th-century reformer Liang Qichao (1873–1929) called “a nation among nations,” the long history of the Silk Road and the colourful, legendary journeys abroad became instrumental in the formation of a modern Chinese nation of unified diversity rooted in an ancient cosmopolitan past. The staged Silk Road theme dance-dramas thus participate in this formation of the Chinese imagination of “nation” and “abroad,” as they aestheticise Chinese history and geography. History and geography—aspects commonly considered constituents of a nation as well as our conceptualisations of “abroad”—are “invariably aestheticized to a certain degree” (Bakhtin 208). Diverse historical and cultural elements from along the Silk Road come together in this performance genre, which can be considered the most representative of various possible stagings of the history and culture of the Silk Road journeys.In 1979, the Chinese state officials in Gansu Province commissioned the benchmark dance-drama Rain of Flowers along the Silk Road, a spectacular theatrical dance-drama praising the pure and noble friendship which existed between the peoples of China and other countries in the Tang dynasty (618-907 C.E.). While its plot also revolves around the Dunhuang Caves and the life of a painter, staged at one of the most critical turning points in modern Chinese history, the work as a whole aims to present the state’s intention of re-establishing diplomatic ties with the outside world after the Cultural Revolution. Unlike Dunhuang, My Dreamland, it presents a nation’s journey abroad and home. To accomplish this goal, Rain of Flowers along the Silk Road introduces the fictional character Yunus, a wealthy Persian merchant who provides the audiences a vision of the historical figure of Peroz III, the last Sassanian prince, who after the Arab conquest of Iran in 651 C.E., found refuge in China. By incorporating scenes of ethnic and folk dances, the drama then stages the journey of painter Zhang’s daughter Yingniang to Persia (present-day Iran) and later, Yunus’s journey abroad to the Tang dynasty imperial court as the Persian Empire’s envoy.Rain of Flowers along the Silk Road, since its debut at Beijing’s Great Hall of the People on the first of October 1979 and shortly after at the Theatre La Scala in Milan, has been staged in more than twenty countries and districts, including France, Italy, Japan, Thailand, Russia, Latvia, Hong Kong, Macao, Taiwan, and recently, in 2013, at the Lincoln Center for the Performing Arts in New York.“The Road”: Staging the Journey TodayWithin the contemporary context of global interdependencies, performing arts have been used as strategic devices for social mobilisation and as a means to represent and perform modern national histories and foreign policies (Davis, Rees, Tian, Tuohy, Wong, David Y. H. Wu). The Silk Road has been chosen as the basis for these state-sponsored, extravagantly produced, and internationally staged contemporary dance programs. In 2008, the welcoming ceremony and artistic presentation at the Olympic Games in Beijing featured twenty apsara dancers and a Dunhuang bihua yuewu dancer with long ribbons, whose body was suspended in mid-air on a rectangular LED extension held by hundreds of performers; on the giant LED screen was a depiction of the ancient Silk Road.In March 2013, Chinese president Xi Jinping introduced the initiatives “Silk Road Economic Belt” and “21st Century Maritime Silk Road” during his journeys abroad in Kazakhstan and Indonesia. These initiatives are now referred to as “One Belt, One Road.” The State Council lists in details the policies and implementation plans for this initiative on its official web page, www.gov.cn. In April 2013, the China Institute in New York launched a yearlong celebration, starting with "Dunhuang: Buddhist Art and the Gateway of the Silk Road" with a re-creation of one of the caves and a selection of artifacts from the site. In March 2015, the National Development and Reform Commission (NDRC), China’s top economic planning agency, released a new action plan outlining key details of the “One Belt, One Road” initiative. Xi Jinping has made the program a centrepiece of both his foreign and domestic economic policies. One of the central economic strategies is to promote cultural industry that could enhance trades along the Silk Road.Encouraged by the “One Belt, One Road” policies, in March 2016, The Silk Princess premiered in Xi’an and was staged at the National Centre for the Performing Arts in Beijing the following July. While Dunhuang, My Dreamland and Rain of Flowers along the Silk Road were inspired by the Buddhist art found in Dunhuang, The Silk Princess, based on a story about a princess bringing silk and silkworm-breeding skills to the western regions of China in the Tang Dynasty (618-907) has a different historical origin. The princess's story was portrayed in a woodblock from the Tang Dynasty discovered by Sir Marc Aurel Stein, a British archaeologist during his expedition to Xinjiang (now Xinjiang Uygur autonomous region) in the early 19th century, and in a temple mural discovered during a 2002 Chinese-Japanese expedition in the Dandanwulike region. Figure 5: Poster of The Silk PrincessIn January 2016, the Shannxi Provincial Song and Dance Troupe staged The Silk Road, a new theatrical dance-drama. Unlike Dunhuang, My Dreamland, the newly staged dance-drama “centers around the ‘road’ and the deepening relationship merchants and travellers developed with it as they traveled along its course,” said Director Yang Wei during an interview with the author. According to her, the show uses seven archetypes—a traveler, a guard, a messenger, and so on—to present the stories that took place along this historic route. Unbounded by specific space or time, each of these archetypes embodies the foreign-travel experience of a different group of individuals, in a manner that may well be related to the social actors of globalised culture and of transnationalism today. Figure 6: Poster of The Silk RoadConclusionAs seen in Rain of Flowers along the Silk Road and Dunhuang, My Dreamland, staging the processes of Silk Road journeys has become a way of connecting the Chinese imagination of “home” with the Chinese imagination of “abroad.” Staging a nation’s heritage abroad on contemporary stages invites a new imagination of homeland, borders, and transnationalism. Once aestheticised through staged performances, such as that of the Dunhuang bihua yuewu, the historical and topological landscape of Dunhuang becomes a performed narrative, embodying the national heritage.The staging of Silk Road journeys continues, and is being developed into various forms, from theatrical dance-drama to digital exhibitions such as the Smithsonian’s Pure Land: Inside the Mogao Grottes at Dunhuang (Stromberg) and the Getty’s Cave Temples of Dunhuang: Buddhist Art on China's Silk Road (Sivak and Hood). 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IntroductionWhat does it mean to be abroad in the modern Australian context? Australia has developed as a country where people increasingly travel both domestically and abroad. Tourism Research Australia reports that 9.6 million resident departures are forecast for 2015-16 and that this will increase to 13.2 million in 2024–25 (Tourism Forecast). This article will identify the development of the Australian culture of travel abroad, the changes that have taken place in Australian society and the conceptual shift of what it means to travel abroad in modern Australia.The traditions of abroad stem from the seventeenth and eighteenth centuries Grand Tour notion where Europeans and Britons travelled on or to the continent to expand their knowledge and experience. While travel at this time focused on history, culture and science, it was very much the domain of the upper classes (Cooper). The concept of the tourist is often credited with Thomas Cook’s first package tour in 1841, which used railways to facilitate trips for pleasure (Cooper). Other advances at the time popularised the trip abroad. Steamships, expanded rail and road networks all contributed to an age of emerging mobility which saw the development of travel to a multi-dimensional experience open to a great many more people than ever before. This article explores three main waves of influence on the Australian concept of abroad and how each has shifted the experience and meaning of what it is to travel abroad.Australians Abroad The post-war period saw significant changes to Australian society, particularly advances in transport, which shaped the way Australians travelled in the 1950s and 1960s. On the domestic front, Australia began manufacturing Holden cars with Prime Minister Ben Chifley unveiling the first Holden “FX” on 29 November 1948. Such was its success that over 500,000 Holden cars were produced by the end of the next decade (Holden). Throughout the 1950s and 1960s the government established a program to standardise railway gauges around the country, making direct travel between Melbourne and Sydney possible for the first time. Australians became more mobile and their enthusiasm for interstate travel flowed on to international transport (Lee).Also, during the 1950s, Australia experienced an influx of migrants from Southern Europe, followed by the Assisted Passage Scheme to attract Britons in the late 1950s and through the 1960s (“The Changing Face of Modern Australia”). With large numbers of new Australians arriving in Australia by ship, these ships could be filled for their return journey to Britain and Europe with Australian tourists. Travel by ship, usually to the “mother country,” took up to two months time, and communication with those “back home” was limited. By the 1960s travelling by ship started to give way to travel by air. The 1950s saw Qantas operate Royal flights for Queen Elizabeth II and the Duke of Edinburgh for their Australian tour, and in 1956 the airline fleet of 34 propeller drive aircraft carried a record number of passengers to the Melbourne Olympics. On 14 January 1958 Qantas launched the first world service from Melbourne flying the Kangaroo Route (via India) and the Southern Cross Route (via the United States) and before long, there were eight such services operating weekly (Qantas). This developing network of international air services connected Australia to the world in a way it had not been previously (Lee).Such developments in Australian aviation were significant on two fronts. Firstly, air travel was a much faster, easier, and more glamorous means of travel (Bednarek) despite the cost, comfort, safety, and capacity issues. The increase in air travel resulted in a steady decline of international travel by boat. Secondly, air travel abroad offered Australians from all walks of life the opportunity to experience other cultures, ideas, fashions, and fads from abroad. These ideas were fed into a transforming Australian society more quickly than they had been in the past.Social change during the late 1950s and into the 1960s connected Australia more closely to the world. The Royal Tour attracted the attention of the British Empire, and the Melbourne Olympics drew international attention. It was the start of television in Australia (1956) which gave Australians connectivity in a way not experienced previously. Concurrent with these advances, Australian society enjoyed rising standards of living, increased incomes, a rise in private motorcar ownership, along with greater leisure time. Three weeks paid holiday was introduced in NSW in 1958 and long service leave soon followed (Piesse). The confluence of these factors resulted in increased domestic travel and arguably altered the allure of abroad. Australians had the resources to travel in a way that they had not before.The social desire for travel abroad extended to the policy level with the Australian government’s 1975 introduction of the Working Holiday Programme (WHP). With a particular focus on young people, its aim was to foster closer ties and cultural exchange between Australia and partner countries (Department of Immigration and Boarder Protection). With cost and time commitments lessened in the 1960s and bilateral arrangements for the WHP in the 1970s, travel abroad became much more widespread and, at least in part, reduced the tyranny of distance. It is against the backdrop of increasingly connected transport networks, modernised communication, and rapid social change that the foundation for a culture of mobility among Australians was further cemented.Social Interactions AbroadDistance significantly shapes the experience of abroad. Proximity has a long association with the volume and frequency of communication exchange. Libai et al. observed that the geographic, temporal, and social distance may be much more important than individual characteristics in communication exchange. Close proximity fosters interpersonal interaction where discussion of experiences can lead to decision-making and social arrangements whilst travelling. Social interaction abroad has been grounded in similarity, social niceties, a desire to belong to a social group of particular travellers, and the need for information (Harris and Prideaux). At the same time, these interactions also contribute to the individual’s abroad experience. White and White noted, “the role of social interaction in the active construction of self as tourist and the tourist experience draws attention to how tourists self-identify social worlds in which they participate while touring” (43). Similarly, Holloway observed of social interaction that it is “a process of meaning making where individuals and groups shape understandings and attitudes through shared talk within their own communities of critique” (237).The unique combination of social interaction and place forms the experiences one has abroad. Cresswell observed that the geographical location and travellers’ sense of place combine to produce a destination in the tourism context. It is against this backdrop of material and immaterial, mobile and immobile, fixed and fluid intersections where social relations between travellers take place. These points of social meeting, connectivity and interaction are linked by way of networks within the destination or during travel (Mavric and Urry) and contribute to its production of unique experiences abroad.Communicating Abroad Communication whilst abroad, has changed significantly since the turn of the century. The merging of the corporeal and technological domains during travel has impacted the entire experience of travel. Those who travelled to faraway lands by ship in the 1950s were limited to letter writing and the use of telegrams for urgent or special communication. In the space of less than 60 years, the communication landscape could not look more different.Mobile phones, tablets, and laptops are all carried alongside the passport as the necessities of travel. Further, Wi-Fi connectivity at airports, on transport, at accommodation and in public spaces allows the traveller to continue “living” at home—at least in the technological sense—whilst physically being abroad. This is not just true of Australians. Global Internet use has grown by 826.9% from 361 million users in 2000 to 3.3 billion users in 2015. In addition, there were 7.1 billion global SIM connections and 243 million machine-to-machine connections by the end of 2014 (GSMA Intelligence). The World Bank also reported a global growth in mobile telephone subscriptions, per 100 people, from 33.9 in 2005 to 96.3 in 2014. This also means that travellers can be socially present while physically away, which changes the way we see the world.This adoption of modern communication has changed the discourse of “abroad” in a number of ways. The 24-hour nature of the Internet allows constant connectivity. Channels that are always open means that information about a travel experience can be communicated as it is occurring. Real time communication means that ideas can be expressed synchronously on a one-to-one or one-to-many basis (Litvin et al.) through hits, clicks, messages, on-line ratings, comments and the like. Facebook, Snapchat, Instagram, WhatsApp, Viber, Twitter, TripAdvisor, blogs, e-mails and a growing number of channels allow for multifaceted, real time communication during travel.Tied to this, the content of communicating the travel experience has also diversified from the traditional written word. The adage that “a picture tells a thousand words” is poignantly relevant here. The imagery contributes to the message and brings with it a degree of tone and perspective and, at the same time, adds to the volume being communicated. Beyond the written word and connected with images, modern communication allows for maps and tracking during the trip. How a traveller might be feeling can be captured with emojis, what they think of an experience can be assessed and rated and, importantly, this can be “liked” or commented on from those “at home.”Technologically-enhanced communication has changed the traveller’s experience in terms of time, interaction with place, and with people. Prior to modern communication, the traveller would reflect and reconstruct travel tales to be recounted upon their return. Stories of adventure and travels could be malleable, tailored to audience, and embellished—an individual’s recount of their individual abroad experience. However, this has shifted so that the modern traveller can capture the aspects of the experience abroad on screen, upload, share and receive immediate feedback in real time, during travel. It raises the question of whether a traveller is actually experiencing or simply recording events. This could be seen as a need for validation from those at home during travel as each interaction and experience is recorded, shared and held up for scrutiny by others. It also raises the question of motivation. Is the traveller travelling for self or for others?With maps, photos and images at each point, comments back and forth, preferences, ratings, records of social interactions with newfound friends “friended” or “tagged” on Facebook, it could be argued that the travel is simply a chronological series of events influenced from afar; shaped by those who are geographically distanced.Liquid Modernity and Abroad Cresswell considered tourist places as systems of mobile and material objects, technologies, and social relations that are produced, imagined, recalled, and anticipated. Increasingly, developments in communication and closeness of electronic proximity have closed the gap of being away. There is now an unbroken link to home during travel abroad, as there is a constant and real time exchange of events and experiences, where those who are travelling and those who are at home are overlapping rather than discrete networks. Sociologists refer to this as “mobility” and it provides a paradigm that underpins the modern concept of abroad. Mobility thinking accepts the movement of individuals and the resulting dynamism of social groups and argues that actual, virtual, and imagined mobility is critical to all aspects of modern life. Premised on “liquid modernity,” it asserts that people, objects, images, and information are all moving and that there is an interdependence between these movements. The paradigm asserts a network approach of the mobile (travellers, stories, experiences) and the fixed (infrastructure, accommodation, devices). Furthermore, it asserts that there is not a single network but complex intersections of flow, moving at different speed, scale and viscosity (Sheller and Urry). This is a useful way of viewing the modern concept of abroad as it accepts a level of maintained connectivity during travel. The technological interconnectivity within these networks, along with the mobile and material objects, contributes to overlapping experiences of home and abroad.ConclusionFrom the Australian perspective, the development of a transport network, social change and the advent of technology have all impacted the experience abroad. What once was the realm of a select few and a trip to the mother country, has expanded to a “golden age” of glamour and excitement (Bednarek). Travel abroad has become part of the norm for individuals and for businesses in an increasingly global society.Over time, the experience of “abroad” has also changed. Travel and non-travel now overlap. The modern traveller can be both at home and abroad. Modernity and mobility have influenced the practice of the overseas where the traveller’s experience can be influenced by home and vice-versa simultaneously. Revisiting the modern version of the “grand tour” could mean standing in a crowded gallery space of The Louvre with a mobile phone recording and sharing the Mona Lisa experience with friends and family at home. It could mean exploring the finest detail and intricacies of the work from home using Google Art Project (Ambroise).While the lure of the unique and different provides an impetus for travel, it is undeniable that the meaning of abroad has changed. In some respects it could be argued that abroad is only physical distance. Conversely overseas travel has now melded into Australian social life in such a way that it cannot be easily unpicked from other aspects. The traditions that have seen Australians travel and experience abroad have, in any case, provided a tradition of travel which has impacted modern, social and cultural life and will continue to do so.ReferencesAustralian Government. Austrade. Tourism Forecasts 2016. Tourism Research Australia, Canberra. 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