Relevant bibliographies by topics / Glycoconjugates – Analysis

Academic literature on the topic 'Glycoconjugates – Analysis'

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Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Glycoconjugates – Analysis"

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Pawar, Nitin J., Ulf Diederichsen, and Dilip D. Dhavale. "Multivalent presentation of carbohydrates by 314-helical peptide templates: synthesis, conformational analysis using CD spectroscopy and saccharide recognition." Organic & Biomolecular Chemistry 13, no. 46 (2015): 11278–85. http://dx.doi.org/10.1039/c5ob01673h.

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Lee, Y. C. "Analysis of Glycoconjugates." Analytical Biochemistry 283, no. 1 (July 2000): 1–2. http://dx.doi.org/10.1006/abio.2000.4642.

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Raju, T. Shantha. "Analysis of Glycoconjugates." Analytical Biochemistry 283, no. 2 (August 2000): 123–24. http://dx.doi.org/10.1006/abio.2000.4646.

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Leducq, R., and C. Gabrion. "Developmental changes of Echinococcus multilocularis metacestodes revealed by tegumental ultrastructure and lectin-binding sites." Parasitology 104, no. 1 (February 1992): 129–41. http://dx.doi.org/10.1017/s003118200006087x.

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SUMMARYUltrastructural investigations (SEM, TEM) combined with lectin-binding analysis, have revealed concurrent modifications in tegumentary structure and surface glycoconjugates during the establishment and differentiation of Echinococcus multilocularis metacestodes in jirds. The laminated layer, which is amorphous and rich in polysaccharides when initially secreted by the young cyst, takes on a different appearance and has a different glycoconjugate composition according to whether the cyst becomes fertile or sterile. The laminated layer of fertile cysts transforms into a microfibrillar matrix, the protein content of which may increase while sugar content decreases during protoscolex differentiation. Independently of this structure, brood capsules, from which arise protoscoleces, are formed by invagination of the cyst tegument. The intense secretion of glycoconjugates from the brood capsule wall during invagination may serve to interact with host factors passing through the laminated layer. The combined use of ultrastructural study and lectin labelling has allowed the demonstration of an ultrastructural and biochemical gradient of differentiation of the protoscolex. Seven stages of differentiation have been described. The possibility that the excreted–secreted tegumentary glycoconjugates, revealed by lectin labelling during protoscolex differentiation, might be the gradual biochemical expression of one or several stimuli implicated in the phenomenon of protoscolex maturation, is discussed.
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Baraniuk, J. N., P. B. Silver, J. D. Lundgren, P. Cole, M. A. Kaliner, and P. J. Barnes. "Bombesin stimulates human nasal mucous and serous cell secretion in vivo." American Journal of Physiology-Lung Cellular and Molecular Physiology 262, no. 1 (January 1, 1992): L48—L52. http://dx.doi.org/10.1152/ajplung.1992.262.1.l48.

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Bombesin, gastrin-related peptide (GRP), and related peptides sharing the common carboxyterminal sequence stimulate lactoferrin (serous cell marker) and glycoconjugate (mucous cell and goblet cell marker) release from human nasal mucosal explants in vitro. In vivo, GRP released from trigeminal sensory nerves may act upon GRP-bombesin binding sites on respiratory epithelial cells and submucosal glands. To determine whether GRP-bombesin can stimulate nasal secretion in vivo, bombesin was administered to eight normal subjects by unilateral, topical administration. Secretions from both nostrils were collected for measurement of total protein, lysozyme, hexose-containing glycoconjugates, and albumin (marker of vascular permeability). Baseline secretions contained 72.0 +/- 17.3 micrograms/ml of total protein, 14 +/- 2 micrograms/ml of lysozyme, 113 +/- 44 micrograms/ml of hexose-containing glycoconjugates, and 7.8 +/- 3.4 micrograms/ml of albumin. Hexose-containing glycoconjugate secretion was significantly increased after 1 nmol (385 +/- 63 micrograms/ml, P less than 0.001 by analysis of variance), 10, 100, and 1,000 nmol of bombesin, but the secretion was not dose dependent. Significant lysozyme (24 +/- 3 micrograms/ml, P less than 0.05) and total protein (155 +/- 23 micrograms/ml, P less than 0.01) secretion occurred after 1,000 nmol. No statistically significant changes in albumin secretion occurred at any dose. Saline had no significant effects on secretion. Therefore, bombesin stimulated secretion from submucosal glands and possibly epithelial cells in the human nose without affecting vascular permeability.
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Lakhtin, V., M. Lakhtin, A. Melikhova, I. Davydkin, V. Davydkin, and E. KIimova. "GLYCOCONJUGATES IN PROGNOSTIC ANALYSIS AGAINST INFECTIONS AND PATHOGENS: THE KEYS TO APPLICATION." ASJ 1, no. 56 (December 29, 2021): 04–11. http://dx.doi.org/10.31618/asj.2707-9864.2021.1.56.142.

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The overview focuses on our own data on the use of water-soluble glycoconjugates (www.lectinity.com) based on a linear polyacrylamide chain in relation to recombinant therapeutic human protein hormones and probiotic recognition proteins such as enzymes and lectins. The results obtained characterize the basic principles of multilevel relationships between proteins and glycoconjugates, including the assembly of complexes and nanoparticles on the solid phase. Prospects for the application of these principles and cases of interaction of proteins and glycoconjugates, including taking into account the participation of enzymes, in the study of human proteins and viruses, are noted. The presented data can help in development of the protective network communication systems as well as new combined preparations against infections and pathogens. These data can serve the keys to be applied in medical biotechnology.
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Gewaily, Mahmoud S., Mohamed Kassab, Asmaa Aboelnour, Essam A. Almadaly, and Ahmed E. Noreldin. "Comparative Cellular Localization of Sugar Residues in Bull (Bos taurus) and Donkey (Equus asinus) Testes Using Lectin Histochemistry." Microscopy and Microanalysis 27, no. 6 (October 12, 2021): 1529–38. http://dx.doi.org/10.1017/s1431927621012939.

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Lectins are glycoproteins of a non-immune origin often used as histochemical reagents to study the distribution of glycoconjugates in different types of tissues. In this study, we performed a comparative cellular localization of sugar residues in bull and donkey testes using immunofluorescent lectin histochemistry. We inspected the cellular localization of the glycoconjugates within the testes using 11 biotin-labeled lectins (LCA, ConA, PNA, WGA, DBA, SBA, ECA, BPL, PTL-II, UEA-1, and PHA-E4) classified under six groups. Although the basic testicular structure in both species was similar, the cellular components showed different lectin localization patterns. The statistical analysis revealed no significant association between the intensity of labeling and different variables, including group and type of lectin and type of cell examined, at p < 0.05. However, a stronger response tended to occur in the donkey than in the bull testes (odds ratio: 1.3). These findings may be associated with the different cellular compositions of the glycoproteins and modification changes during spermatogenesis. Moreover, glycoconjugate profiling through lectin histochemistry can characterize some cell-type selective markers that will be helpful in studying bull and donkey spermatogenesis.
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Bazhenova, Aleksandra, Fang Gao, Barbara Bolgiano, and Stephen E. Harding. "Glycoconjugate vaccines against Salmonella enterica serovars and Shigella species: existing and emerging methods for their analysis." Biophysical Reviews 13, no. 2 (April 2021): 221–46. http://dx.doi.org/10.1007/s12551-021-00791-z.

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AbstractThe global spread of enteric disease, the increasingly limited options for antimicrobial treatment and the need for effective eradication programs have resulted in an increased demand for glycoconjugate enteric vaccines, made with carbohydrate-based membrane components of the pathogen, and their precise characterisation. A set of physico-chemical and immunological tests are employed for complete vaccine characterisation and to ensure their consistency, potency, safety and stability, following the relevant World Health Organization and Pharmacopoeia guidelines. Variable requirements for analytical methods are linked to conjugate structure, carrier protein nature and size and O-acetyl content of polysaccharide. We investigated a key stability-indicating method which measures the percent free saccharide of Salmonella enterica subspecies enterica serovar Typhi capsular polysaccharide, by detergent precipitation, depolymerisation and HPAEC-PAD quantitation. Together with modern computational approaches, a more precise design of glycoconjugates is possible, allowing for improvements in solubility, structural conformation and stability, and immunogenicity of antigens, which may be applicable to a broad spectrum of vaccines. More validation experiments are required to establish the most effective and suitable methods for glycoconjugate analysis to bring uniformity to the existing protocols, although the need for product-specific approaches will apply, especially for the more complex vaccines. An overview of current and emerging analytical approaches for the characterisation of vaccines against Salmonella Typhi and Shigella species is described in this paper. This study should aid the development and licensing of new glycoconjugate vaccines aimed at the prevention of enteric diseases.
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Güther, Maria Lucia Sampaio, Kenneth Beattie, Douglas J. Lamont, John James, Alan R. Prescott, and Michael A. J. Ferguson. "Fate of Glycosylphosphatidylinositol (GPI)-Less Procyclin and Characterization of Sialylated Non-GPI-Anchored Surface Coat Molecules of Procyclic-Form Trypanosoma brucei." Eukaryotic Cell 8, no. 9 (July 24, 2009): 1407–17. http://dx.doi.org/10.1128/ec.00178-09.

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ABSTRACT A Trypanosoma brucei TbGPI12 null mutant that is unable to express cell surface procyclins and free glycosylphosphatidylinositols (GPI) revealed that these are not the only surface coat molecules of the procyclic life cycle stage. Here, we show that non-GPI-anchored procyclins are N-glycosylated, accumulate in the lysosome, and appear as proteolytic fragments in the medium. We also show, using lectin agglutination and galactose oxidase-NaB3H4 labeling, that the cell surface of the TbGPI12 null parasites contains glycoconjugates that terminate in sialic acid linked to galactose. Following desialylation, a high-apparent-molecular-weight glycoconjugate fraction was purified by ricin affinity chromatography and gel filtration and shown to contain mannose, galactose, N-acetylglucosamine, and fucose. The latter has not been previously reported in T. brucei glycoproteins. A proteomic analysis of this fraction revealed a mixture of polytopic transmembrane proteins, including P-type ATPase and vacuolar proton-translocating pyrophosphatase. Immunolocalization studies showed that both could be labeled on the surfaces of wild-type and TbGPI12 null cells. Neither galactose oxidase-NaB3H4 labeling of the non-GPI-anchored surface glycoconjugates nor immunogold labeling of the P-type ATPase was affected by the presence of procyclins in the wild-type cells, suggesting that the procyclins do not, by themselves, form a macromolecular barrier.
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Maass, Kai, René Ranzinger, Hildegard Geyer, Claus-Wilhelm von der Lieth, and Rudolf Geyer. "“Glyco-peakfinder” - de novo composition analysis of glycoconjugates." PROTEOMICS 7, no. 24 (December 2007): 4435–44. http://dx.doi.org/10.1002/pmic.200700253.

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Dissertations / Theses on the topic "Glycoconjugates – Analysis"

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Ismail, Mohd Nazri. "Glycomic analysis of biomedically important O-glycoconjugates." Thesis, Imperial College London, 2011. http://hdl.handle.net/10044/1/6217.

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It has been established that all cells carry an array of glycans attached to proteins and lipids that are crucial in the interaction between cells and the surrounding matrix. Proteins are mainly glycosylated on asparagines (N-glycosylation) and serine or threonine residues (O-glycosylation). Compared to N-glycans, O-glycans offer a higher degree of structural ambiguity due to the existence of several types and cores. This is believed to contribute to the relative lack of knowledge on these molecules. Therefore, improvement to the current methodologies of structural studies is a prerequisite to complement the immense functional findings of O-glycoconjugates in biological systems. This thesis discusses the structural characterisation, regulation and biological roles of O-glycans. The overall aim was to optimise O-glycomic mass spectrometric analysis to help illuminate the phenotypic findings from our collaborators in three separate but related projects. The methodologies utilised involving MALDI-TOF/TOF-MS, GC-EI-MS, ESI-QTOF-MS and MALDI-QIT-TOF-MS. The first project investigated the effects of core 2 GlcNAc transferase (C2GnT) deficiency in mice. This enzyme exists in three isoforms which are expressed differently in different tissues. Analysis of the single knockout of each of these isoenzymes as well as the triple knockouts has allowed the investigation of their unique and overlapping functions. The outcomes of this study include characterisation of alterations of not just mucin-type O-glycans but also O-mannose glycan, which could be associated with several organ lesions and system failures. The second project focused on the gastric mucosa of mice with deficiency in α1,2-fucosyltranferase (FuT2). This enzyme plays an important role in decorating the mucosal mucins with ABH-blood group and Lewis antigens that are known to interact with various gut flora including the pathogen Helicobacter pylori. It has been shown that the binding of H. pylori via BabA adhesins was significantly impaired with the loss of H antigens and Lewis y on O-glycans. The third project investigated the regulation of mucin-type O-glycosylation. The protein Src has been recognised to play an essential role in the localisation of ppGalNAc transferases, the initiating enzyme of O-glycosylation, in the endoplasmic reticulum and Golgi apparatus. Therefore, it could be inferred that Src influences the regulation of protein O-glycosylation. The NIH3T3 and NBT-II cell lines with different levels of Src or different localisation of ppGalNAcT-2 have been analysed in order to identify the changes on the structures of O-glycans and the relative abundances of cores 1 and 2. Valuable information has been gathered which could lead to further investigative work to better understand the role of Src in the regulation of protein O-glycosylation.
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Klockow-Beck, Antje. "Analysis of carbohydrates in food and glycoconjugates with capillary electrophoresis /." [S.l.] : [s.n.], 1995. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=11241.

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Faenzi, Elisa <1981&gt. "Analysis of the memory B cell response against glycoconjugate vaccines." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4472/.

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The development of vaccines directed against polysaccharide capsules of S. pneumoniae, H. influenzae and N. meningitidis have been of great importance in preventing potentially fatal infections. Bacterial capsular polysaccharides are T-cell-independent antigens that induce specific antibody response characterized by IgM immunoglobulins, with a very low IgG class switched response and lack of capability of inducing a booster response. The inability of pure polysaccharides to induce sustained immune responses has required the development of vaccines containing polysaccharides conjugated to a carrier protein, with the aim to generate T cell help. It is clear that the immunogenicity of glycoconjugate vaccines can vary depending on different factors, e.g. chemical nature of the linked polysaccharide, carrier protein, age of the target population, adjuvant used. The present study analyzes the memory B cell (MBC) response to the polysaccharide and to the carrier protein following vaccination with a glycoconjugate vaccine for the prevention of Group B streptococcus (GBS) infection. Not much is known about the role of adjuvants in the development of immunological memory raised against GBS polysaccharides, as well as about the influence of having a pre-existing immunity against the carrier protein on the B cell response raised against the polysaccharide component of the vaccine. We demonstrate in the mouse model that adjuvants can increase the antibody and memory B cell response to the carrier protein and to the conjugated polysaccharide. We also demonstrate that a pre-existing immunity to the carrier protein favors the development of the antibody and memory B cell response to subsequent vaccinations with a glycoconjugate, even in absence of adjuvants. These data provide a useful insight for a better understanding of the mechanism of action of this class of vaccines and for designing the best vaccine that could result in a productive and long lasting memory response.
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Mogemark, Mickael. "Solid-phase glycoconjugate synthesis : on-resin analysis with gel-phase ¹9F NMR spectroscopy." Doctoral thesis, Umeå University, Chemistry, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-438.

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An efficient and versatile non-destructive method to analyze the progress of solid-phase glycoconjugate synthesis with gel-phase 19F NMR spectroscopy is described. The method relies on use of fluorinated linkers and building blocks carrying fluorinated protective groups. Commercially available fluorinated reagents have been utilized to attach the protective groups.

The influence of resin structures for seven commercial resins upon resolution of gel-phase 19F NMR spectra was investigated. Two different linkers for oligosaccharide synthesis were also developed and successfully employed in preparation of α-Gal trisaccharides and a n-pentenyl glycoside. Finally, reaction conditions for solid-phase peptide glycosylations were established.

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Anderson, Taigh Byrne. "Towards the Synthesis of the Unusual Monosaccharides Found in the Shigella sonnei O-Antigen and Analysis of Shigella flexneri 2a Glycoconjugate Vaccine Samples." Thesis, Faculty of Science, 2021. http://hdl.handle.net/11427/33192.

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Shigellosis (bacillary dysentery) is a severe inflammatory diarrhoeal disease in humans caused by the Gram-negative bacteria belonging to the Shigella species. Despite over 60 years of vaccine research, no licensed vaccine to prevent shigellosis is commercially available. Bioconjugate vaccines based on the O-antigen against various Shigella serotypes are under development. Shigella sonnei and Shigella flexneri 2a are the most prevalent serotypes in industrialised and developed countries respectively and is the subject of this study. This project involves the design and evaluation of alternative synthetic routes to derivatives of 2-acetamido-4-amino-2,4,6-trideoxy-β-D-galactopyranose (FucNAc4N/AAT) and 2-acetamido2-deoxy-α-L-altruronic acid (AltNAcA), the two unusual monosaccharides found in the repeating unit of the Shigella sonnei O-antigen. Since these sugars are not commercially available, synthetic derivatives are required as authentic standards for the analysis of the bioconjugate. Various routes to the FucNAc4N derivative were investigated and evaluated. Routes proceeding either through 1,6-anhydro-D-glucose or cyclohexyl-2-acetamido-1-thioglucoside were shown to have potential, but ultimately both were rejected on the basis of inefficient conversions in the early stages of the synthetic sequence. However, important insights were gained into the crucial challenge of differentiating O-3 and O-4, common to any approach involving starting materials with the D-gluco configuration. This led to preparation in good yield of phenyl 2-amino-2-N,3-Ocarbonyl-2-deoxy-1-thio-β-D-glucopyranoside as a key oxazolidinone-protected intermediate, which allowed for successful preparation of a FucNAc4N derivative in the form of a 4-azido-βthioglycoside. This was achieved in 10 steps from the commercially available 2-acetamido1,3,4,6-tetra-O-acetyl-β-D-glucopyranose in an overall yield of 17%. Synthesis of an AltNAcA derivative was initially investigated via a sequence starting from a glucofuranurono-3,6-lactone. This involved initial inversion at C-5 followed by opening of the lactone and migration of the substituent at O-5 to O-3, to form an idofuranuronate which, however, could not be readily converted to the required pyranose form. A more successful route utilized a 6-iodo-2,3-oxazolidinone derivative of D-glucose, prepared as a key intermediate in the synthesis of FucNAc4N. The crucial epimerization at C-5 was attempted through initial formation of the 5-ene, followed by a hydroboration/oxidation, but this led exclusively to the Drather than the L-sugar. Computer modelling and literature precedent suggested that the anomeric configuration strongly influenced the face selectivity of the hydroboration step. An αanalogue of the 6-iodo-2,3-oxazolidinone derivative was therefore prepared via an efficient Lewis acid catalysed anomerization of a β-thioglucoside. However attempts to carry out a base- iii mediated elimination to the corresponding 5-enopyranoside were not successful, giving rise instead to a product in which the oxazolidinone had been cleaved followed by intramolecular substitution of the 6-iodide to form a 3,6-anhydro derivative. On the basis of these results and observations, an alternative synthetic route to AltNAcA has been proposed, which incorporates early formation of an α-glycoside and removal of the useful 2,3-oxazolidinone protecting group, thus setting the substrate up for effective elimination followed by selective hydroboration from the less hindered β-face to give the L-sugar. This study also incorporates a spectroscopic analysis of Shigella flexneri 2a glycoconjugate and glycopeptide samples. A full set of nuclear magnetic resonance (NMR) spectra were recorded and analysed, resulting in the unambiguous determination of the structure and integrity of the O-antigen saccharide component.
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van, der Hulst Lieke. "The analysis of grapevine response to smoke exposure." Thesis, 2018. http://hdl.handle.net/2440/113378.

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Smoke taint is a fault found in wines made from grapes exposed to bushfire smoke. It is characterised by objectionable smoky and ashy aromas and flavours, which have been attributed to the presence of smoke derived volatile phenols, in free and glycoconjugate forms. Chapter 1 comprises a summary of the impact of bushfires on the wine industry and a review of previous smoke taint research, which includes many investigations into the composition of wine produced from smoke-affected fruit. Gaps of knowledge are identified in Chapter 1, and the issues addressed in this thesis are identified and summarised in the research aims. Chapter 2 describes a field trial that investigated the accumulation of smoke taint precursors in three Vitis vinifera cultivars, Sauvignon Blanc, Chardonnay and Merlot, at different time points, following grapevine exposure to smoke under experimental conditions. Varietal differences in volatile phenol glycoconjugate profiles were observed; interestingly, these profiles also differed between samples harvested 1 day after smoke exposure and samples harvested at maturity. An evaluation of the effect of an agrichemical applied to grapevine fruit and foliage as a physical barrier to prevent the uptake of smoke is also reported; together with the results of an investigation into the potential for reflectance spectroscopy, measured using a handheld spectrometer, to detect smoke-affected fruit. A subsequent field trial sought to further verify the use of a second agrichemical to mitigate the impacts of grapevine exposure to smoke; and reflectance spectroscopy to evaluate smoke exposure in the vineyard and is also included in Chapter 2. Whereas the glycosylation of smoke derived volatile phenols in grapevine fruit and leaves following exposure to smoke is reasonably well understood, the biochemical and molecular consequences of grapevine smoke exposure have received comparatively little consideration. The research described in Chapter 3 endeavours to address this knowledge gap through investigations into the expression of grapevine glycosyltransferases (GTs) following smoke exposure. Higher expression profiles of certain sets of genes (including heat shock proteins and putative GTs) were identified through RNA sequencing of two grape cultivars grown as potted grapevines in a growth room. Selected GT candidates were analysed in a subsequent field trial, in which Q-PCR expression analysis showed higher expression of two GT1 family genes at specific time points; with differential expression found to be highest in skin, rather than pulp, fractions following smoke exposure. To date, the occurrence of smoke taint has not been reported in crops other than grapes, despite the proximity of bushfires in regions comprising broader agricultural production. The final chapter of experimental work in this thesis, Chapter 4, describes analysis of a field trial involving the application of smoke to apple trees, to investigate whether or not apples can be similarly affected by smoke. Chapter 5 reflects on the experimental work described in this thesis, including a discussion towards challenges and future directions in the research of smoke taint.
Thesis (Ph.D.) (Research by Publication) -- University of Adelaide, School of Agriculture, Food and Wine, 2018
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Shih, Han. "Step-growth thiol-ene photopolymerization to form degradable, cytocompatible and multi-structural hydrogels." Thesis, 2014. http://hdl.handle.net/1805/3842.

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Indiana University-Purdue University Indianapolis (IUPUI)
Hydrogels prepared from photopolymerization have been used for a variety of tissue engineering and controlled release applications. Polymeric biomaterials with high cytocompatibility, versatile degradation behaviors, and diverse material properties are particularly useful in studying cell fate processes. In recent years, step-growth thiol-ene photochemistry has been utilized to form cytocompatible hydrogels for tissue engineering applications. This radical-mediated gelation scheme utilizes norbornene functionalized multi-arm poly(ethylene glycol) (PEGNB) as the macromer and di-thiol containing molecules as the crosslinkers to form chemically crosslinked hydrogels. While the gelation mechanism was well-described in the literature, the network properties and degradation behaviors of these hydrogels have not been fully characterized. In addition, existing thiol-ene photopolymerizations often used type I photoinitiators in conjunction with an ultraviolet (UV) light source to initiate gelation. The use of cleavage type initiators and UV light often raises biosafety concerns. The first objective of this thesis was to understand the gelation and degradation properties of thiol-ene hydrogels. In this regard, two types of step-growth hydrogels were compared, namely thiol-ene hydrogels and Michael-type addition hydrogels. Between these two step-growth gel systems, it was found that thiol-ene click reactions formed hydrogels with higher crosslinking efficiency. However, thiol-ene hydrogels still contained significant network non-ideality, demonstrated by a high dependency of hydrogel swelling on macromer contents. In addition, the presence of ester bonds within the PEGNB macromer rendered thiol-ene hydrogels hydrolytically degradable. Through validating model predictions with experimental results, it was found that the hydrolytic degradation of thiol-ene hydrogels was not only governed by ester bond hydrolysis, but also affected by the degree of network crosslinking. In an attempt to manipulate network crosslinking and degradation rate of thiol-ene hydrogels, different macromer contents and peptide crosslinkers with different amino acid sequences were used. A chymotrypsin-sensitive peptide was also used as part of the hydrogel crosslinkers to render thiol-ene hydrogels enzymatically degradable. The second objective of this thesis was to develop a visible light-mediated thiol-ene hydrogelation scheme using a type II photoinitiator, eosin-Y, as the only photoinitiator. This approach eliminates the incorporation of potentially cytotoxic co-initiator and co-monomer that are typically used with a type II initiator. In addition to investigating the gelation kinetics and properties of thiol-ene hydrogels formed by this new gelation scheme, it was found that the visible light-mediated thiol-ene hydrogels were highly cytocompatible for human mesenchymal stem cells (hMSCs) and pancreatic MIN6 beta-cells. It was also found that eosin-Y could be repeatedly excited for preparing step-growth hydrogels with multilayer structures. This new gelation chemistry may have great utilities in controlled release of multiple sensitive growth factors and encapsulation of multiple cell types for tissue regeneration.
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Books on the topic "Glycoconjugates – Analysis"

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J, Jiménez-Barbero, and Peters T, eds. NMR spectroscopy of glycoconjugates. Weinheim: Wiley-VCH, 2003.

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1944-, Jackson P., and Gallagher J. T, eds. A laboratory guide to glycoconjugate analysis. Basel: Birkhäuser Verlag, 1997.

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L, Burlingame A., ed. Mass spectrometry: Modified proteins and glycoconjugates. Amsterdam: Elsevier Academic Press, 2005.

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F, Hounsell Elizabeth, ed. Glycoanalysis protocols. 2nd ed. Totowa, N.J: Humana Press, 1998.

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Jackson, Peter, and John T. Gallagher, eds. A Laboratory Guide to Glycoconjugate Analysis. Basel: Birkhäuser Basel, 1995. http://dx.doi.org/10.1007/978-3-0348-7388-8.

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Diagnostic assays for colon cancer. Boca Raton: CRC Press, 1991.

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1951-, Townsend R. Reid, and Hotchkiss Arland T, eds. Techniques in glycobiology. New York: M. Dekker, 1997.

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Ziad, El Rassi, ed. Carbohydrate analysis: High performance liquid chromatography and capillary electrophoresis. Amsterdam: Elsevier, 1995.

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Jackson, P., and J. T. Gallagher. Laboratory Guide to Glycoconjugate Analysis. Birkhauser, 2014.

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Jackson, P., and J. T. Gallagher. Laboratory Guide to Glycoconjugate Analysis. Birkhauser Verlag, 2012.

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Book chapters on the topic "Glycoconjugates – Analysis"

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Yamaguchi, Yoshiki, Takumi Yamaguchi, and Koichi Kato. "Structural Analysis of Oligosaccharides and Glycoconjugates Using NMR." In Advances in Neurobiology, 165–83. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1154-7_8.

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Gray, Christopher, and Sabine L. Flitsch. "Methods for the High Resolution Analysis of Glycoconjugates." In Coupling and Decoupling of Diverse Molecular Units in Glycosciences, 225–67. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-65587-1_11.

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Yamaguchi, Yoshiki, Takumi Yamaguchi, and Koichi Kato. "Structural Analysis of Oligosaccharides and Glycoconjugates Using NMR." In Advances in Neurobiology, 163–84. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-12390-0_6.

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Costello, Catherine E., Hélène Perreault, and Lambert C. Ngoka. "Mass Spectrometric and Tandem Mass Spectrometric Approaches to the Analysis of Glycoconjugates." In Mass Spectrometry in the Biological Sciences, 365–84. Totowa, NJ: Humana Press, 1996. http://dx.doi.org/10.1007/978-1-4612-0229-5_19.

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Ravenscroft, Neil, Paolo Costantino, Philippe Talaga, Roberto Rodriguez, and William Egan. "Glycoconjugate Vaccines." In Vaccine Analysis: Strategies, Principles, and Control, 301–81. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-662-45024-6_8.

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Klockow-Beck, Antje, and Aran Paulus. "Carbohydrate Analysis with Capillary Electrophoresis." In A Laboratory Guide to Glycoconjugate Analysis, 141–60. Basel: Birkhäuser Basel, 1997. http://dx.doi.org/10.1007/978-3-0348-7388-8_8.

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Neue, Uwe D., and Charles H. Phoebe. "High-Performance Liquid Chromatography of Derivatized and Non-Derivatized Oligosaccharides: A Review." In A Laboratory Guide to Glycoconjugate Analysis, 1–22. Basel: Birkhäuser Basel, 1997. http://dx.doi.org/10.1007/978-3-0348-7388-8_1.

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Linhardt, Robert J., Toshihiko Toida, April E. Smith, and Ronald E. Hileman. "Analysis of the Structure of Heparin and Heparan Sulfate by High-Resolution Separation of Oligosaccharides." In A Laboratory Guide to Glycoconjugate Analysis, 183–97. Basel: Birkhäuser Basel, 1997. http://dx.doi.org/10.1007/978-3-0348-7388-8_10.

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Guile, Geoffrey R., Pauline M. Rudd, David R. Wing, and Raymond A. Dwek. "HPLC Strategies for Profiling and Sequencing Oligosaccharides." In A Laboratory Guide to Glycoconjugate Analysis, 199–234. Basel: Birkhäuser Basel, 1997. http://dx.doi.org/10.1007/978-3-0348-7388-8_11.

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Prime, Sally B., Nigel F. Shipston, and Tony H. Merry. "Enzymatic Sequence Analysis of Glycoprotein Glycans." In A Laboratory Guide to Glycoconjugate Analysis, 235–60. Basel: Birkhäuser Basel, 1997. http://dx.doi.org/10.1007/978-3-0348-7388-8_12.

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Conference papers on the topic "Glycoconjugates – Analysis"

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Saheb, Entsar, Olga Tarasenko, and Olga Tarasenko. "COMPARATIVE ANALYSIS OF IMMUNE CELLS ACTIVATION AND CYTOTOXICITY UPON EXPOSURE PATHOGEN AND GLYCOCONJUGATES." In BIOLOGY, NANOTECHNOLOGY, TOXICOLOGY AND APPLICATIONS: 4th BioNanoTox (Biology, Nanotechnology, Toxicology) and Applications. AIP, 2010. http://dx.doi.org/10.1063/1.3419693.

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Xuan, Trinh Anh, Phan Nghia Trung, Bui Long Dinh, Takumi Yamaguchi, and Koichi Kato. "Preparation of water-soluble glycoconjugated poly(acrylamide) for NMR analyses of carbohydrate-carbohydrate interactions." In PROCEEDINGS OF PPS-29: The 29th International Conference of the Polymer Processing Society - Conference Papers. American Institute of Physics, 2014. http://dx.doi.org/10.1063/1.4873797.

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You might also be interested in the extended bibliographies on the topic 'Glycoconjugates – Analysis' for particular source types:
Journal articles
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