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1

Affan, Muhammad. "Effect of Salt Content (NaCl) on the Antagonistic Power of Gliocladium sp. in Inhibiting the growth of Fusarium oxysporum f. sp. Cubense Causes Fusarium wilt in Banana Plants." Contributions of Central Research Institute for Agriculture 17, no. 1 (January 30, 2023): 14–19. http://dx.doi.org/10.35335/cceria.v17i1.39.

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This study aims to determine the effect of salt/salinity (NaCl) levels on the growth of Gliocladium sp. (Colony area, dry weight and number of spores) and antagonistic activity of Gliocladium sp. in inhibiting Foc. The research was conducted at the Laboratory of Plant Diseases, Department of Plant Pests and Diseases, Faculty of Agriculture, University of Brawijaya. The stages in this study included counting colony area, sporulation and dry weight of Gliocladium sp. with single culture method, antagonism test between Gliocladium sp. and Foc with dual culture method. In the research stage to determine the effect of salt levels used NaCl, with concentrations of 0 gr/l, 4 gr/l, 8 gr/l, 12 gr/l and 16 gr/l. The design method used in this study was a completely randomized design (CRD). Banana plants can grow in tropical climates with a pH of 4.5-7. In banana cultivation there are several obstacles that can reduce banana production, the main obstacle is caused by the attack of the pathogen Fusarium oxysporum f. sp cubense (Foc) which causes banana plants to wilt. Efforts to control the attack of pathogenic Foc, can be done by utilizing the antagonistic fungus Gliocladium sp. The results showed that the colony area, sporulation and dry weight of Gliocladium sp. showed that overall growth increased with increasing salt content used, the results obtained were colony area, sporulation and dry weight of Gliocladium sp. The highest yield was found in the treatment with a concentration of salt content of 16 gr/l, with values of 4.184 cm2, 7.933 spores/ml and 0.132 gr respectively, while the antagonism test between Gliocladium sp. and Foc, the highest inhibition percentage of Gliocladium sp. occurred at a salt concentration of 16 gr/l of 45.556%. The research shows that the addition of NaCl to the media has a positive effect on Gliocladium sp.
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2

Emilda, Deni, and Mizu Istianto. "Pengaruh Minyak Cengkeh terhadap Pertumbuhan Koloni dan Sifat Antagonis Cendawan Gliocladium sp. terhadap Fusarium oxysporum f. sp. cubense." Jurnal Hortikultura 21, no. 1 (October 13, 2016): 33. http://dx.doi.org/10.21082/jhort.v21n1.2011.p33-39.

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Minyak cengkeh dan cendawan Gliocladium sp. diketahui memiliki potensi sebagai agens pengendali cendawan Fusarium oxysporum f. sp. cubense (Foc), penyebab penyakit layu pada tanaman pisang. Pengujian kompatibilitas antara minyak cengkeh dengan cendawan Gliocladium sp. perlu dievaluasi dalam rangka menyusun paket pengendalian terpadu terhadap penyakit layu Fusarium. Tujuan penelitian ialah mengevaluasi pengaruh minyak cengkeh terhadap pertumbuhan koloni cendawan Gliocladium sp. dan daya hambatnya terhadap cendawan Foc ras 4. Penelitian dilaksanakan di Laboratorium Proteksi Tanaman Balai Penelitian Tanaman Buah Tropika pada bulan Mei sampai Juli 2008. Perlakuan terdiri atas minyak cengkeh volume 3, 9, dan 18 µl disusun dalam rancangan acak lengkap dengan lima ulangan. Hasil penelitian menunjukkan bahwa miselium cendawan Gliocladium sp. yang mendapat perlakuan minyak cengkeh masih mampu tumbuh hingga memenuhi ruang cawan petri. Jumlah konidia cendawan Gliocladium sp. yang diperlakukan dengan minyak cengkeh terbukti lebih sedikit dibanding cendawan yang tidak diperlakukan dengan minyak cengkeh. Cendawan Gliocladium sp. yang telah mendapat perlakuan minyak cengkeh masih memiliki sifat antagonistik yang efektif terhadap cendawan Foc. Efektivitas antagonisme cendawan tersebut tidak berbeda nyata dengan efektivitas antagonisme yang tidak diperlakukan dengan minyak cengkeh. Hasil ini memberikan harapan karena minyak cengkeh tidak memberikan efek negatif terhadap aktivitas Gliocladium sp., sehingga dapat dijadikan sebagai salah satu komponen dalam program pengendalian secara terpadu penyakit layu Fusarium pada pisang. Namun masih diperlukan penelitian lebih lanjut mengenai pengaruh minyak cengkeh terhadap pertumbuhan tanaman.<br /><br /><br />Clove essential oil and Gliocladium sp. are known to have the potency for controlling Fusarium oxysporum f.sp cubense (Foc) the causal agent of wilt banana disease. The compatibility of clove oil and Gliocladium sp. has to be evaluated to establish an integrated pest management against Fusarium disease. The objective of this experiment was to evaluate the effect of clove oil on the growth of Gliocladium sp. colony and the inhibition value of this fungus to Foc race 4. The experiment was conducted in the Plant Protection Laboratory of Indonesian Tropical Fruit Research Institute from May to July 2008. The treatments were volumes of clove oil i.e. 3, 9, and 18 µl that were arranged in a completely randomized design with five replications. The results showed that Gliocladium sp. mycelia treated with clove oil could still grow throughout the available space within petridish. However, Gliocladium sp. treated with this oil had lower number of conidia than that it was untreated. Gliocladium sp. treated with clove oil had still effective antagonism trait to Foc. This effectiveness was not significantly different from Gliocladium sp. that was untreated with clove oil. This result indicated that clove oil had good potency as a component in integrated control program against wilt disease on banana, because it did not have negative effect to Gliocladium sp. However, further research is still needed to evaluate the effect of clove oil to plant growth. <br /><br />
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3

Hidayat, Taufiq, Ahmad Syauqi, and Tintrim Rahayu. "Uji Antagonis Jamur Gliocladium sp dalam Menghambat Pertumbuhan Jamur Fusarium sp Penyebab Penyakit Layu Pada Tanaman Pisang (Musa paradisiaca L.)." BIOSAINTROPIS (BIOSCIENCE-TROPIC) 5, no. 2 (January 9, 2020): 59–65. http://dx.doi.org/10.33474/e-jbst.v5i2.257.

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The fungus Gliocladium sp. is the microbial soil that can be isolated from the root zone of banana plant (Musa Paradica L.) Those fungi are included in the group of potentially antagonistic microbes in the growth inhibiting of Fusarium sp fungus which infects the plants and it cause disease on banana crops withered. This research aim was to know the difference of growth inhibiting percentage of Gliocladium sp, to the Fusarium sp. fungus at pH 5.5 and pH 7. This research uses experiment methods. The results of the growth inhibiting percentage in the last day was higher at pH 5.5 of 35.2% whereas at pH 7 of 14%. This indicates that the growth of the fungus is more suitable in pH acidic approach because of its nature which is able to adjust to the fungal metabolism. The inhibition mechanism of the Gliocladium sp. on fungus inhibiting of Fusarium sp. has the stages of the competition, lysis and mycoparasit. Keywords: Gliocladium sp., Fusarium sp, banana plants, pH ABSTRAK Jamur Gliocladium sp. merupakan mikroba tanah yang dapat diisolasi dari daerah perakaran tanaman pisang (Musa Paradica L.) Jamur tersebut termasuk dalam kelompok mikroba antagonis yang berpotensi dalam menghambat pertumbuhan jamur Fusarium sp yang menginfeksi tanaman yang menyebabkan penyakit layu pada tanaman pisang. Penelitian ini bertujuan untuk mengetahui perbedaan persentase daya hambat jamur Gliocladium sp. dalam menghambat pertumbuhan jamur Fusarium sp. pada pH 5,5 dan pH 7. Penelitian ini menggunakan metode deskriptif dan eksperimen. Hasil dari persentase daya hambat hari terakhir lebih tinggi pada pH 5,5 sebesar 35,2% sedangkan pada pH 7 sebesar 14%. Hal ini menandakan bahwa pertumbuhan jamur lebih cocok di pH mendekati asam karena sifatnya yang mampu menyesuaikan dengan metabolisme jamur. Mekanisme Penghambatan jamur Gliocladium sp. kepada jamur Fusarium sp. mempunyai tahap kompetisi, lisis dan mikoparasit. Kata kunci: Gliocladium sp., Fusarium sp., tanaman pisang, pH
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4

PARDEDE, MEI NOVITA BR, GUSTI NGURAH ALIT SUSANTA WIRYA, and KHAMDAN KALIMI. "Efektivitas Trichoderma sp. dan Gliocladium sp. untuk Pengendalian Penyakit Busuk Batang (Fusarium Oxysporum Sp.) pada Tanaman Vanili (Vanilla Planifolia)." Agrotrop : Journal on Agriculture Science 12, no. 1 (May 31, 2022): 63. http://dx.doi.org/10.24843/ajoas.2022.v12.i01.p06.

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Effectiveness of Trichoderma sp. and Gliocladium sp. For Controlling Stem Rot (Fusarium Oxysporum sp.) in Vanilla (Vanilla planifolia). Vanilla (Vanilla planifolia Andrews) is one of the industrial plants that have very high economic value as an export commodity. One of the main obstacles to increasing vanilla production is stem rot disease. Stem rot disease caused by Fusarium oxysporum f. sp. vanillae became one of the main obstacles faced. This research on the effectiveness of antagonistic microbes aims to obtain the best antagonist microbes in suppressing stem rot disease in vanilla plants. The experiment was carried out in vitro at the Laboratory of Plant Diseases at Udayana University and in vivo at the Experimental Garden Greenhouse. The experiment used a randomized block design with six replications and four treatments, namely Control (without antagonistic microbes), G (Gliocladium sp. + compost), T (Trichoderma sp. + compost), and GT (Gliocladium sp. and Trichoderma sp. + compost). The results showed that T (Trichoderma sp. + compost) and GT (Gliocladium sp. and Trichoderma sp. + compost) able inhibit stem rot disease with the best percentage of 10%.
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5

Malik, Akhmad Faisal, Romauli Siagian, and Farriza Diyasti. "Penghambatan Gliocladium sp. Cordo. terhadap Phytophthora capsici Leonian. Penyebab Penyakit Busuk Pangkal Batang Lada Serta Bentuk Formulasinya." AGROSCRIPT: Journal of Applied Agricultural Sciences 4, no. 1 (June 25, 2022): 39–47. http://dx.doi.org/10.36423/agroscript.v4i1.972.

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Penyakit busuk pangkal batang (BPB) pada tanaman lada yang disebabkan cendawan Phytophthora capsici merupakan penyakit penting yang dapat menyebabkan kehilangan hasil hingga 15% per tahun. Pemanfaatan agens hayati merupakan alternatif pengendalian penyakit tanaman yang ramah lingkungan. Penelitian ini bertujuan untuk menganalisis kemampuan penghambatan Gliocladium sp. terhadap P. capsici secara in vitro serta mengetahui kemampuannya sebagai dekomposer. Metode penelitian menggunakan metode dual culture antara Gliocladium sp. dengan P. capsici. Sementara itu, biokompos dibuat dengan bahan dasar serbuk gergaji-dedak dengan perbandingan 3:2 untuk mengetahui kemampuan Gliocladium sp. sebagai dekomposer. Hasil penelitian menunjukkan bahwa Gliocladium sp. mampu menghambat pertumbuhan P. capsici dengan persentase penghambatan tertinggi sebesar 54,89% setelah lima hari inokulasi. Di sisi lain, Gliocladium juga mampu mendekomposisi dan tumbuh baik pada bahan organik berupa campuran serbuk gergaji-dedak
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6

Sopialena. "Test Of The Effectiveness Of Antagonic Fungi In The Control Of Colletotrichum Mushrooms In Tomato Plants (Lycopersicum esculentum L.). In Vitro." Agrifarm : Jurnal Ilmu Pertanian 9, no. 2 (December 7, 2020): 10–14. http://dx.doi.org/10.24903/ajip.v9i2.980.

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This study was conducted to test the effectiveness of several antagonistic fungi (Trichoderma sp., Penicillium sp., Gliocladium sp., And Rhizopus sp) in controlling the pathogenic Colletotrichumsp. fungi from tomato plants (Lycopersicon esculentum L.). in vitro. The research was carried out for 2 (two) months at the Laboratory of Pest and Plant Disease Laboratory, Faculty of Agriculture, Mulawarman University. This research was compiled using a Completely Randomized Design (CRD) consisting of 4 (four) treatments, namely Trichoderma sp., Penicillium sp., Gliocladium sp., And Rhizopus sp. Each treatment was repeated 10 times. Data analysis using Analysis of variance and if the results are obtained significantly different then further tested using the Least Significant Difference. The results showed that the most effective antagonistic fungus suppressed the growth of the pathogen Colletotrichum sp. is Gliocladium sp. and Trichoderma sp., meanwhile the antagonist fungus Rhizopus sp., which is less effective in suppressing the growth of the fungus Colletotrichum sp., and the fungus Penicillium sp., are the most ineffective fungi in suppressing the growth of the pathogenic Colletotrichum sp. Key words: Trichoderma sp., Penicillium sp., Gliocladium sp., And Rhizopus sp., Colletotrichum sp., Tomatoes (Lycopersicon esculentum L.) Keywords :Pyricularia oryza Cav., Endophyte fungi, Antagonistic mechanim
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7

Kalimutu, Putu Krisnawan, Ida Bagus Komang Mahardika, and Putri Risa Adriani Anak Agung Sagung. "Antagonism Test of Trichoderma atroviride and Gliocladium sp. Bali Local Isolates As a Disease Control of Blendok Disease (Botryodiplodia theobromae) in Grapefruit (Citrus grandis L. Osbeck)." SEAS (Sustainable Environment Agricultural Science) 4, no. 2 (November 2, 2020): 102–10. http://dx.doi.org/10.22225/seas.4.2.2311.102-110.

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This study aims to determine the ability of Trichoderma atroviride and Gliocladium sp. in inhibiting the fungus that causes Gumosis disease Botryodiplodia theobromae. This study used a completely randomized design with two single treatments and was repeated eight times. In the in-vitro antagonism test Trichoderma atroviride and Gliolcadium sp. can inhibit the fungus Botryodiplodia theobromae as well. The percentage of inhibition was 94.58% in the Trichoderma atroviride antagonism test and 81.67% in the Gliocladium sp. antagonism test. Whereas the Botryodiplodia theobromae Colony Area had no significant effect on both treatments. Each Botryodiplodia theobromae Colony area is 18.34 cm2 with T. atroviride treatments and 22.25 cm2 with Gliocladium sp. treatment. The growth rate of Botryodiplodia Theobroma fungi was superior to that of Trichoderma atroviride and Gliocladium sp.
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8

Octriana, Liza. "Potensi Agen Hayati dalam Menghambat Pertumbuhan Phytium sp. secara In Vitro." Buletin Plasma Nutfah 17, no. 2 (October 11, 2016): 138. http://dx.doi.org/10.21082/blpn.v17n2.2011.p138-142.

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<p>The Potential of Biological Agents to Inhibit Growth of Phytium sp. In Vitro. The study aimed at testing the potential of some antagonistic fungi isolated from durian seedlings media to inhibit growth of Phytium sp. Research was done at the Central Laboratory of Tropical Fruit Research Solok in July-September 2009 by using a complete randomized design with 5 treatments and 4 replications. Tests was conducted by dual culture method on PDA. The results showed that Gliocladium sp., Trichoderma sp.a, Trichoderma sp.b, Aspergilus sp., and Penicillium sp. can inhibit growth of Phytium sp., with growth inhibition of 50, 49.5, 47, 48, and 38.3% respectively. Inhibition mecanism of Gliocladium sp., and Trichoderma sp. were competition, antibiosis, lisis, and parasitism, while Penicillium sp. was antibiosis. Gliocladium sp., Trichoderma sp.a, Trichoderma sp.b, Aspergilus sp., and Penicillium sp. can be used as biological agents to control pathogenic fungi Phytium sp.</p><p> </p><p><strong>Abstrak</strong></p><p>Penelitian bertujuan untuk menguji potensi beberapa cendawan antagonis hasil isolasi dari media pembibitan durian dalam menghambat pertumbuhan Phytium sp. Penelitian dilakukan di Laboratorium Balai Penelitian Tanaman Buah Tropika Solok pada bulan Juli-September 2010. Penelitian disusun dalam rancangan acak lengkap dengan 5 perlakuan dan 4 ulangan. Pengujian daya antagonis cendawan dilakukan dengan metode dual culture yang diinokulasikan pada media PDA. Hasil penelitian menunjukkan bahwa Gliocladium sp., Trichoderma sp.a, Trichoderma sp.b., Aspergilus sp., dan Penicillium sp. dapat menghambat pertumbuhan Phytium sp. secara in vitro, dengan daya hambat masing-masing 50; 49,5; 47; 48; dan 38,3% secara berurutan. Mekanisme antagonis Gliocladium sp. dan Trichoderma sp. adalah kompetisi, antibiosis, lisis, dan parasitisme, sedangkan Penicillium sp. hanya bersifat antibiosis. Gliocladium sp., Trichoderma sp., Aspergilus sp., dan Penicillium sp. dapat digunakan sebagai agen hayati untuk mengendalikan cendawan patogen Phytium sp.</p>
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Krisnadi, Krisnadi, R. Wahyono Widodo, and Agus Surya Mulya. "Pengaruh Dosis Bioaktivator Gliocladium Sp Terhadap Pertumbuhan Benih Kopi Varietas Sigarar Utang Dan Lini S 795 Di Perbenihan." Paspalum: Jurnal Ilmiah Pertanian 8, no. 1 (April 28, 2020): 57. http://dx.doi.org/10.35138/paspalum.v8i1.178.

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The important thing in coffee plantation ventures is when preparing coffee seeds such as Sigarar debt and line S 795. Planting Media plays an important role to get good seeds. Bioaktivator Gliocladium sp which is a fungus that can accelerate the process of decomposition of organic matter in nature. The method of research used is experimental design. Research conducted in Plantation PPPPTK Agriculture Cianjur Ministry of Education and Culture. The research draft using a separate tile experiment (Split Plot design) consists of two treatments. The first treatment (main plot) consists of 2 of the Varitas Sigarar debt and line S 795. The second treatment (plot) consists of 5 levels. There are 10 treatment combinations and each of them is 3 repeats so there are 30 units of trial. The number of seeds in research 1500 plants. The free variable i.e. the main tile (v) consists of 2 levels namely V1 = Debt Sigarar Varieties v2 = varieties of line S 795. Child Tile (d) consists of 5 levels ie D0 = 0 gr/kg media cropping (control), D1 = 1 gr/kg of media cropping, D2 = 2 G gr/kg of media planting, D3 = 3 gr/kg of media planting, D4 = 4 gr/kg of planting media. The use of Sigararutang varieties and lines 795 S and the bioactivator dose of Gliocladiun there is no interaction. The results of the self-test varieties all the parameters do not differ real only on the high parameter plant variety line S 795 gives a different plant height than the Sigararutang varieties. Self-test result dose of bioaktiovator Gliocladium SP dose 4 gr/kg of medium gives high results on all parameters. On the parameters of wet-weights plant dose Bioaktiovator Gliocladium sp not differ in real. Optimum dose of Bioaktiovator Gliocladium sp which produces maximum dry weight respectively on each coffee varieties at a dose of 4 gr/kg of media on the Sigararutang varieties provide a dry weight of 2.42 grams, higher than in line S 795 of 2.24 grams.
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Indaryaningsih, Novalia, Antok Wahyu Sektiono, and Ika Rochdjatun Sastrahidayat. "IDENTIFIKASI PENYAKIT HAWAR DAUN PADA DRASENA (Dracaena sp.). SERTA UJI PENGHAMBATANNYA MENGGUNAKAN JAMUR ANTAGONIS SECARA IN VITRO." Jurnal Hama dan Penyakit Tumbuhan 9, no. 2 (June 30, 2021): 65–71. http://dx.doi.org/10.21776/ub.jurnalhpt.2021.009.2.5.

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Hawar daun adalah salah satu penyakit yang ditemukan menyerang tanaman drasena. Alternatif pengendalian penyakit yang ramah lingkungan ini adalah memanfaatkan mikroba antagonis terhadap jamur patogen. Penelitian ini bertujuan untuk mengidentifikasi jamur patogen penyebab hawar daun pada tanaman drasena (Dracaena sp.) dan mengetahui perbedaan daya hambat jamur antagonis yang terdiri atas Gliocladium sp., Aspergillus sp., dan Fusarium sp. terhadap jamur patogen. Percobaan dilakukan menggunakan metode rancangan acak lengkap (RAL) yang terdiri dari 4 perlakuan dengan tiga kali ulangan. Pengujian dilakukan dengan cara menumbuhkan potongan biakan murni jamur patogen dan jamur antagonis pada cawan Petri berdiameter 9 cm. Hasil penelitian menunjukkan bahwa jamur penyebab penyakit hawar daun yang menyerang tanaman hias drasena (Dracaena sp.) adalah jamur Gloeosporium sp. Di saat yang sama, hasil uji antagonis menunjukkan bahwa jamur, Gliocladium sp., Aspergillus sp., dan Fusarium sp. dapat menghambat pertumbuhan jamur Gloeosporium sp. Gliocladium sp. memiliki daya hambat tertinggi yaitu sebesar 64,33%.
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Ruliyanti, Winda, and Abdul Majid. "Pengaruh Pemberian Vermikompos pada Media Tanam Terhadap Efektivitas Gliocladium sp. dalam Mengendalikan Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Semangka (Citrulus vulgaris, Schard)." Jurnal Pengendalian Hayati 3, no. 1 (March 18, 2020): 14. http://dx.doi.org/10.19184/jph.v3i1.17147.

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Watermelon is one of the plants that have good prospects and has high economic value, but its production is currently experiencing a decline. Decreased production caused by the pathogen Fusarium oxysporum which causes fusarium wilt disease in plants. losses caused by fusarium wilt attacks reach 20-30%. Utilization of Gliocladium sp. and vermicompost is an alternative step in controlling fusarium wilt. The results showed that the use of Gliocladium sp. and vermicompost can suppress the development of fusarium wilt disease. The use of Gliocladium sp. and vermicompost 250 grams/polybag can suppress the attack of fusarium wilt disease with a percentage of suppression of the incidence of disease by 70% and suppress the severity of the disease by 38.5% compared to controls. and has a control effectiveness value of 73.68% which is categorized very well. The use of Gliocladium sp. and vermicompost also affect plant growth and production.
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Dailah, Siti, Mofit Eko Poerwanto, and Supono Budi Sutoto. "EFFECTIVENESS OF ANTAGONISTIC FUNGI Gliocladium sp. AND Trichoderma harzianum TO CONTROL Colletotrichum spp. on chili (Capsicum annuum L.)." Agrivet 26, no. 1 (January 9, 2021): 17. http://dx.doi.org/10.31315/agrivet.v26i1.4306.

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The productivity of chili in terms of both quality and quantity is suppressed by the attacks of anthracnose disease caused by Colletotrichum spp. Pesticides is commonly used to control the disease. Researched was conducted to determine the antagonistic fungi and the effective dose in inhibiting the growth of the Colletotrichum spp. Antagonistic tests were performed in vivo using the direct method with three replicates. Inhibition test was arranged in a Complete Randomized Block Design (RCBD) with 7 treatments: Control, Gliocladium sp (150, 250, and 350 mL/plant) and T. harzianum (150, 250, and 350 mL/plant) with four replicates and three samples out of 20 plants per treatment. The results showed that the percentage of inhibition of T. harzianum against Colletotrichum sp. is 55% while Gliocladium sp. is 70.33% in antagonistic test. The mean of disease intensity at 11 up to 32 DAI on control is 5.61 ± 0.71% up to 20.30 ± 1.35 % respectively. It is significantly higher than Gliocladium sp. 350 mL/Plant (1.94 ± 0.45 % up to 11.15 ± 0.87 %), T. harzianum 350 mL/Plant (1.10 ± 0.58 % up to 8.68 ± 0.91 %), and T. harzianum 250 mL/Plant (2.28 ± 0.43 % up to 8.75 ± 0.79 %). No significant different is observed on mean of fresh weight of red chili fruit yield per plant. T. harzianum and Gliocladium sp. fungi are effective in controlling the attack of anthracnose disease in chili. The most efficient dose to control anthracnose disease is the application of T. Harzianum or Gliocladium sp at the dose of 250 mL/plant.Key Words: Chilli, Anthracnose, Gliocladium, Trichoderma, Colletotrichum.
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Sopialena, Sopialena, Suyadi Suyadi, Sofian Sofian, Devi Tantiani, and Aziz Nur Fauzi. "EFEKTIVITAS CENDAWAN ENDOFIT SEBAGAI PENGENDALI PENYAKIT BLAST PADA TANAMAN PADI (Oryza sativa)." AGRIFOR 19, no. 2 (September 30, 2020): 355. http://dx.doi.org/10.31293/af.v19i2.4813.

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Penelitian dilakukan untuk menentukan: cendawan endofit yang terdapat pada tanaman padi (Oryza sativa); persentase daya hambat cendawan endofit terhadap cendawan Pyricularia oryzae Cav.; dan mekanisme antagonis antara masing-masing cendawan endofit terhadap cendawan Pyricularia oryzae Cav.. Penelitian dilakukan di laboratorium terpadu Fakultas Pertanian Universitas Mulawarman, dan sampel tanaman yang terinfeksi serta tanaman sehat dikumpulkan dari Kelurahan Sungai Kapih, Kecamatan Sambutan, Kota Samarinda. Hasil penelitian disusun dalam Rancangan Acak Lengkap (RAL), yang terdiri dari empat sampel dan setiap sampel diulang sebanyak sepuluh kali. Hasil penelitian menunjukkan bahwa cendawan endofit yang terisolasi dari tanaman padi (Oryza sativa) adalah Trichoderma sp., Rhizopus sp., Gliocladium sp. dan Penicillium sp. Kompetisiterbukti secara in vitro sebagai mekanisme antagonis Rhizopus sp., sedangkan antibiosis terbukti secara in vitro sebagai mekanisme Penicillium sp.. Trichoderma sp dan Gliocladium sp terbukti memiliki dua mekanisme antagonis yaitu kompetisi dan parasitisme. Kemampuan cendawan endofit sebagai agen biologis untuk bersaing dengan cendawan P. oryzae itu menghasikan perbedaan daya antagonisme, tingkat tertinggi penghambatan terjadi padaP.oryzae vs Gliocladium sp. (78,96%).
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Georgieva, Olga, Natalia Karadzhova, and Slavka Kalapchieva. "Application of biological products at cultivation of garden pea Pisum sativum l." Agricultural Sciences 13, no. 29 (June 7, 2021): 118–25. http://dx.doi.org/10.22620/agrisci.2021.29.014.

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The possibilities of using the biological products “Trichodermine” (Trichoderma viride Pers ex Fr.), “Gliocladine” (Gliocladium virens Miller et al.) and the mycorrhizal activator “Rhizo VAM Basic” (Glomus intraradices Schenck&Smith) for control of fungal diseases in garden pea and and their impact on the biometric indicators of young plants were studied. Biological preparations based on antagonistic fungi Trichoderma viride and Gliocladium virens improve the phytosanitary condition of the plants, the mycorrhizal fungus Glomus intraradices stimulates the formation of the root system and tuber formation, but has a weak fungicidal effect against Fusarium wilt in this culture. The experiment was performed on an artificial infectious background by Fusarium oxysporum Schlecht. f. sp. pisi (Hal.) Raillo, isolated from diseased plants. The effect of application of the biological products“Trichodermine” and “Gliocladine” is 34-68%, from “Rhizo VAM Basic” - 8% compared to the infected control. The combined application of biological preparations with fungicidal and mycorrhizal action significantly protects the crop from infection with the causative agent of Fusarium wilt of garden pea.
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Castillo, Humberto, Randall Rojas, and Manuel Villalta. "Actividad antagonista de Gliocladium sp. contra Sclerotium cepivorum." Revista Tecnología en Marcha 29, no. 7 (September 16, 2016): 57. http://dx.doi.org/10.18845/tm.v29i7.2706.

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Algunos géneros fúngicos han sido utilizados para el control de agentes causantes de enfermedades en plantas, por lo que su actividad representa una alternativa para la disminución e incluso la sustitución de químicos sintéticos. Existen hongos antagonistas ampliamente estudiados, como Trichoderma sp., que concurre con otros hongos que poseen un alto potencial como agentes biocontroladores, entre ellos se destaca Gliocladium sp. En este estudio se pretende establecer la capacidad antagonista que tiene Gliocladium contra el patógeno causante de la pudrición blanca de la cebolla, Sclerotium cepivorum. Se logró aislar Gliocladium sp. a partir de una muestra de suelo dedicada al cultivo de cebolla (Allium cepa L.), se subcultivó hasta obtener un cultivo axénico y se realizaron cultivos duales en placas con el hongo y el patógeno. Se determinó que el porcentaje de inhibición del crecimiento radial (PICR) promedio obtenido a los ocho días de exposición (53,66%) no presentaba diferencias significativas (p=0,19) con el obtenido a los 15 días (54,20%) y, dadas las características de inhibición, se presume que el mecanismo de control presentado por la cepa de Gliocladium sp. utilizada responde a la producción de enzimas, metabolitos y posiblemente una mezcla de compuestos orgánicos volátiles capaces de inhibir el crecimiento y desarrollo de S. cepivorum aun sin que sus hifas entren en contacto.
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Afriani, Astri, Maria Heviyanti, and Fitra syawal Harahap. "Effectiveness of gliocladium virens in controlling Fusarium oxysporum F. sp. capsici disease on chilli plant." Jurnal Pertanian Tropik 6, no. 3 (December 1, 2019): 403–11. http://dx.doi.org/10.32734/jpt.v6i3.3187.

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Wilt disease which is caused by Fusarium oxysporum f. sp. capsici is one of the importantdiseases in chili plants that can reduce growth, fruits yield, quality, and chili production. Thisfungus comes inside the vascular bundle through the root tissue and quickly colonizes in xylemvessels and causing typical wilt symptoms on plants. In line with the development of organicfarming systems, biological control by using biological agents is the prospective method incontrolling diseases on the plant. Gliocladium virens are one of the biological agents that obstruct thespreading of disease. The aim of this study was to determine the effectiveness of Gliocladiumvirens in controlling Fusarium oxysporum f. Sp. Capsici on chili plant. This research usedrandomized block design with two factors: 1 The Time application of Gliocladium virens (W)factor; 2. Dosages of Gliocladium virens (D). The results show that on the treatment time ofapplication of Gliocladium virens W1 of 0.06, W2 of 0.05, W3 of 0.09 and W4 of 0.08 were notsignificantly different. The treatment given Gliocladium virens disease intensity at week 3 waslower that is equal to 0.125% compared to without treatment of G. virens (D0) the intensity ofthe disease at week 3 was higher at 1%.
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Castillo, Humberto, Randall Rojas, and Manuel Villalta. "Gliocladium sp., agente biocontrolador con aplicaciones prometedoras." Revista Tecnología en Marcha 29, no. 7 (September 16, 2016): 65. http://dx.doi.org/10.18845/tm.v29i7.2707.

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La mayoría de estudios relacionados con Gliocladium se han enfocado en su potente capacidad como agente biocontrolador, principalmente de hongos fitopatógenos. Sin embargo, las aplicaciones potenciales de este género de hongos abarcan numerosos productos obtenidos a partir de su metabolismo, capaz de generar una gran variedad de compuestos químicos. Cabe destacar también que es un organismo endófito que, además, de proveer beneficios a un amplio rango de plantas hospederas, puede imitar con éxito su comportamiento químico. Entre las aplicaciones más novedosas destaca su potencial para la producción de compuestos asociados con biocombustibles y su capacidad bioabsorbente de metales pesados. Esta investigación describe y ejemplifica estas aplicaciones y muestra a este género de hongos como una prometedora herramienta biotecnológica.
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Huang, Y. F., L. Tian, H. M. Hua, and Y. H. Pei. "Two diketopiperazines from marine fungus Gliocladium sp. YUP08." Journal of Asian Natural Products Research 9, no. 3 (May 1, 2007): 197–201. http://dx.doi.org/10.1080/10286020500531878.

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Usman, N., H. I. Atta, and M. B. Tijjani. "Biodegradation Studies of Benzene, Toluene, Ethylbenzene and Xylene (BTEX) Compounds by Gliocladium sp. and Aspergillus terreus." Journal of Applied Sciences and Environmental Management 24, no. 6 (July 17, 2020): 1063–69. http://dx.doi.org/10.4314/jasem.v24i6.19.

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Benzene, toluene, ethylbenzene and xylene (BTEX) are monoaromatic hydrocarbons found frequently in petroleum and its derivatives; and they are among the most important pollutants of soil and groundwater. This study focused on harnessing the enzymatic capabilities of filamentous fungi Gliocladium sp. and Aspergillus terreus, dwelling in a petroleum-contaminated soil to degrade benzene, toluene, ethylbenzene and xylene (BTEX) compounds. The biodegradation experiment was carried out using the fungi individually and in consortium in a batch culture containing mineral salts medium supplemented with 1% v/v BTEX. The experiments were carried out in triplicates at room temperature on a rotary shaker (180rpm) for twenty five days and aliquots were taken on a five day interval to determine the hydrocarbon utilizing fungal (HUF) count and residual BTEX in order to monitor the rate of biodegradation. The hydrocarbon utilizing fungal counts were determined by direct counting using a Neubauer Haemocytometer while, the residual BTEX was determined using absorbance values measured using a spectrophotometer and the corresponding concentrations determined from a standard curve. The highest percentage degradation of BTEX was observed with Aspergillus terreus (89.1%) while, the least was observed with Gliocladium sp. (84.4%). The growth peak was attained on the 15th day in all treatments after which the HUF counts declined. Statistical analysis showed no significant difference (P>0.05) in the mean amounts of BTEX degraded and hydrocarbon-utilizing fungal counts between the treatments. The strains of Gliocladium sp. and Aspergillus terreus used in this study showed high ability for BTEX degradation thus, they are potential candidates for bioremediation of soils contaminated with monoaromatic hydrocarbons. Keywords: Biodegradation, BTEX, Gliocladium sp., Aspergillus terreus, Monoaromatic hydrocarbons
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Soenartiningsih, Soenartiningsih, Nurasiah Djaenuddin, and M. Sujak Saenong. "Efektivitas Trichoderma sp. dan Gliocladium sp. sebagai Agen Biokontrol Hayati Penyakit Busuk Pelepah Daun pada Jagung." Jurnal Penelitian Pertanian Tanaman Pangan 33, no. 2 (August 28, 2014): 129. http://dx.doi.org/10.21082/jpptp.v33n2.2014.p129-135.

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Sheath blight is an important disease in corn. The disease could cause significant yield loss when infection occurs on susceptible varieties. Disease control using the microorganism antagonist is an alternative for disease management. Research was carried out in a laboratory, greenhouse and field from 2010 to 2012. The research objective was to compare several biological agents for controlling sheath blight disease on corn. In vitro laboratory tests identified that out of sixteen isolates of microorganisms, only 3 isolates which had the potency to suppress the pathogen of sheath blight over 50%, namely TT1; TM; and GM. Conidia development among the three isolates of microorganism the highest was by TT1. In the greenhouse, three isolates of microorganisms showed potential of decreasing sheath blight disease up to 70%. The Gliocladium isolates decreases the disease by 53%. Research results from the field indicated the antagonist had decreased sheath blight disease by 67%. Isolates of Trichoderma and Gliocladium fungus could reduce the yield loss by 23% by suppressing the infection of sheath blight disease.
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Hadiwiyono, S. H. Poromarto, Supyani, S. Widono, and D. N. Septariani. "Disease incidence of fusarium wilt in organic garlic cultivation with compost plus Gliocladium on endemic land." IOP Conference Series: Earth and Environmental Science 1114, no. 1 (December 1, 2022): 012004. http://dx.doi.org/10.1088/1755-1315/1114/1/012004.

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Abstract Fusarium wilt (Fusarium oxysporum f.sp. cepae) is the most important soil-borne disease in garlic. This pathogen is a weak parasite so that it can be controlled through healthy plant cultivation approaches such as the use of organic compost fertilizer and soil microbes as biological control agents. Gliocladium sp. is an antagonist fungus that can be used as a soil borne disease control agent. This study aims to evaluate the effectiveness of compost plus Gliocladium to control garlic root rot in endemic land. The experiment was carried out in Tawangmangu Karanganyar at 1000 meters asl. The experiment consisted of 5 treatments arranged by completely randomized design with three replications. The main variables are disease incidence of fusarium wilt and control efficacy. The other variables observed were the fresh and dry weight of the biomass and the yield in the form of the weight of garlic bulbs per plant. Data processing carried out was analysis of variance and DMRT at 5% level. The results showed that compost plus Gliocladium produced higher effectiveness than compost alone or Gliocladium alone. The effectiveness of compost plus Gliocladium was more effective in suppressing fusarium wilt compared to farmers using fungicides and artificial chemical fertilizers.
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Nuryani, Wakiah, Evi Silfia Yusuf, Hanudin, Ika Djatnika, and Budi Marwoto. "Pengendalian Layu Fusarium Menggunakan Mikrobe Antagonis dan Tanaman Resisten pada Lili." Jurnal Hortikultura 21, no. 4 (December 2, 2011): 338. http://dx.doi.org/10.21082/jhort.v21n4.2011.p338-343.

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Lili merupakan tanaman hias penting dan bernilai ekonomi tinggi. Budidaya lili di Indonesia menghadapi kendala utama yaitu penyakit layu Fusarium. Aplikasi bahan kimia sintetik untuk mengendalikan penyakit ini berdampak negatif terhadap kerusakan lingkungan. Aplikasi organisme antagonis dan kultivar resisten merupakan alternatif pengendalian penyakit ramah lingkungan, berdampak positif terhadap kelestariannya, dan meningkatkan produktivitas lili. Penelitian bertujuan untuk mendapatkan informasi mikrobe antagonis, mikrobe nonpatogenik, dan klon lili yang dapat menekan penyakit layu Fusarium. Penelitian dilakukan di Laboratorium Biokontrol dan Rumah Sere, Balai Penelitian Tanaman Hias pada bulan Januari sampai dengan Desember 2007. Tata letak percobaan disusun berdasarkan rancangan acak kelompok pola faktorial denga tiga ulangan. Faktor pertama ialah klon lili yang terdiri dari klon No.1, No 2, No.3 (tahan), dan No.4 (rentan). Faktor kedua ialah perlakuan mikrobe antagonis (1) Gliocladium sp. 107 spora/ml, (2). Trichoderma sp. 107 sel/ml, (3) Fusarium a virulen 107 spora/ml dan, (4) kontrol (tanpa mikrobe antagonis). Hasil percobaan menunjukkan bahwa penggunaan mikrobe antagonis Gliocladium sp. dan klon resisten paling efektif mengendalikan penyakit busuk umbi Fusarium pada lili. Hal ini dibuktikan dari persentase tanaman layu pada perlakuan aplikasi mikrobe dan penggunaan tanaman resistant dibandingkan dengan tanpa perlakuan. Penanaman tanaman resisten diikuti dengan aplikasi Gliocladium paling efektif menekan layu Fusarium dibanding perlakuan lainnya.<br /><br /><br /><br />Lilium is one of the most important and the heigest economic value of cut flower in Indonesia. Cultivation of Lilium faces major constrain, that is wilt disease caused by Fusarium. Application of synthetic chemicals to control the diseases is not recommended, because its impact is dangerous for environment and human life. Therefore other control measures that are move environmentally friendly and more effective/efficient have to applied. The use of antagonistic microbes and resistance varieties are the most promising control measures to be recommended in the field. The study was aimed to find out of antagonist and nonpathogenic microbes and plant resistant that were effective to control Fusarium bulb rot on lili. The experiment was conducted at Laboratory and Glasshouse of Indonesian Ornamental Crops Research Institute (1,100 m asl.) on January to December 2007. Factorial experiment was arranged in a randomized block design with three replications. The first factor was Lilium clones i.e. No.1, No. 2, No. 3 (resistant), and No. 4 (susceptible clone as control). The second factor was antagonism microbes i.e. (1) Gliocladium sp. 107 spora/ml, (2). 107 Trichoderma sp. cel/ml, (3) Fusarium nonpathogenic 107 spora/ml, and (4) control (tap water without antagonist microbe). The results indicated that the use of Gliocladium sp. and resistant clone of Lilium i.e. clone No.3 was effective to control Fusarium bulb rot of Lilium. This proven from lower percentage of disease occurance on the treatment of antagonistics microbes and resistance varieties compared both to those of without treatment (control). The use of resistance plant followed by application of Gliocladium was known to be most effective to control fusarial wilt disease compared to the other treatment combinations. <br /><br />
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Hartal, Hartal, Misnawaty Misnawaty, and Indah Budi. "EFEKTIVITAS Trichoderma sp. DAN Gliocladium sp. DALAM PENGENDALIAN LAYU FUSARIUM PADA TANAMAN KRISAN." Jurnal Ilmu-Ilmu Pertanian Indonesia 12, no. 1 (November 16, 2017): 7–12. http://dx.doi.org/10.31186/jipi.12.1.7-12.

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Carmo, Ana Lídia Moura do, Edson José Mazaratto, Bárbara Eckstein, and Álvaro Figueredo dos Santos. "Associação de Fungos com Sementes de Espécies Florestais Nativas." Summa Phytopathologica 43, no. 3 (September 2017): 246–47. http://dx.doi.org/10.1590/0100-5405/2211.

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RESUMO No Brasil, ainda há poucas pesquisas sobre os microrganismos associados às sementes florestais. O objetivo desse trabalho foi detectar fungos associados às sementes de seis espécies florestais nativas: açoita-cavalo (Luehea divaricata), araçá amarelo (Psidium cattleyanum), corticeira (Erythrina falcata), rabo-de-bugio (Lonchocarpus sp.), vassoura-vermelha (Dodonea viscosa) e pau-jacaré (Piptadenia gonoacantha). Foram identificados oito gêneros de fungos potencialmente patogênicos: Cladosporyum sp., Fusarium sp., Pestalotiopsis sp., Phomopsis sp., Coletotrichum sp., Alternaria sp., Phoma sp. e Botrytis sp. e fungos saprófitas: Aspergillus sp., Penicillium sp., Rhizopus sp., Epicoccum sp., Torula sp., Trichoderma sp., Gliocladium sp., Chaetomium sp. e Trichothecium sp. Os resultados evidenciam a diversidade de fungos associados às sementes florestais nativas, incluindo vários potencialmente fitopatogênicos.
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Nelson, Carol L., John D. Castello, and Paul D. Manion. "Preliminary investigation of the use of indirect immunofluorescence to detect conidia of Brunchorstiapinea." Canadian Journal of Forest Research 19, no. 3 (March 1, 1989): 389–92. http://dx.doi.org/10.1139/x89-060.

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An indirect immunofluorescence staining procedure was developed for detection of Brunchorstiapinea conidia, using antiserum to conidia and a commercially prepared fluorescein isothiocyanate protein A conjugate. The procedure detected B. pinea conidia from culture, pycnidia, and spore-trap collections. Although cross reactivity occurred with spores of Fusarium spp., Sirococcus sp., Phialophora sp., Gliocladium sp., Verticillium sp., and Gelatinosporium sp., these spores were easily distinguished from those of B. pinea by size, shape, septation, and degree of fluorescence. Fluorescent B. pinea like conidia were collected in spore traps located within and outside the New York State quarantine region. However, the identity of B. pinea like conidia could not be corroborated by other methods.
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Shiomi, Kazuro, Noriko Arai, Yuzuru Iwai, Andreas Turberg, Heinz Kölbl, and Satoshi Ōmura. "Structure of argifin, a new chitinase inhibitor produced by Gliocladium sp." Tetrahedron Letters 41, no. 13 (March 2000): 2141–43. http://dx.doi.org/10.1016/s0040-4039(00)00099-x.

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Rusli, Jumriani, Hafsan Hafsan, and Eka Sukmawaty. "Efek antagonis jamur rhizosfer terhadap jamur patogen tanaman kentang." Filogeni: Jurnal Mahasiswa Biologi 1, no. 1 (April 8, 2021): 1–6. http://dx.doi.org/10.24252/filogeni.v1i1.20546.

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Kentang merupakan tanaman pertanian penting karena merupakan sumber karbohidrat yang menjadi bahan makanan pokok di Indonesia. Namun produktivitasnya terancam oleh cendawan patogen oleh karena itu diperlukan usaha pengendalian hayati menggunakan cendawan antagonis. Penelitian ini bertujuan untuk mengetahui aktivitas antagonis cendawan Rhizosfer terhadap cendawan patogen pada tanaman kentang. Cendawan Rhizosfer kentang diisolasi dan diuji daya antagonisnya menggunakan biakan ganda. Sebanyak 22 isolat cendawan berhasil diisolasi dari Rhizosfer tanaman kentang, dan 3 isolat berpotensi menghambat pertumbuhan F. oxysporum dan 5 isolat berpotensi menghambat pertumbuhan Culvularia sp. Aktivitas penghambatan ditunjukkan oleh 8 isolat cendawan yaitu Aspergillus sp. (3 isolat), Nigrospora sp. (1 isolat), Gliocladium sp. (1 isolat) Penicillum (1 isolat) dan Rizopus sp. (3 isolat) menunjukkan kemampuan kompetisi.
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Wicaksono, Danar, and Maftuh Kafiya. "Penghambatan berbagai isolat Trichoderma sp. terhadap perkecambahan spora Colletotrichum sp." Agro Wiralodra 5, no. 1 (January 11, 2022): 20–27. http://dx.doi.org/10.31943/agrowiralodra.v5i1.74.

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Penyakit antraknosa pada buah cabai menyebabkan kualitas hasil menurun dan kehilangan hasil yang tinggi. Penyakit ini disebabkan oleh jamur Colletotrichum sp. Pada umumnya, antraknosa pada cabai dikendalikan dengan aplikasi fungisida kimia sintetis pada seluruh permukaan buah. Hal ini sangat berbahaya bila dilakukan menjelang panen karena buah cabai juga dikonsumsi segar. Agens pengendali hayati merupakan alternatif pengendalian Colletotrichum sp. pada buah cabai. Penelitian bertujuan untuk memperoleh isolat agens pengendali hayati yang dapat menghambat perkecambahan spora Colletotrichum sp. penyebab penyakit antraknosa pada buah cabai besar keriting. Penelitian diawali dengan isolasi Colletotrichum sp. dari cabai rawit, besar, dan merah keriting. Isolasi menghasilkan dua buah isolat dari cabai merah besar, 5 isolat diisolasi dari cabai merah keriting, dan 7 isolat dari cabai rawit. Uji virulensi dilakukan untuk memperoleh isolat yang paling virulen terhadap cabai merah keriting. Isolat BA asal cabai besar diketahui memiliki virulensi paling tinggi dibandingkan yang lainnya. Tujuh Trichoderma, 1 Gliocladium, dan 2 APH yang belum diidentifikasi digunakan biakan dalam media cair. Suspensi APH digunakan untuk mengendalikan penyakit antraknosa pada buah cabai merah keriting. Penambahan suspensi LPT2 menyebabkan persentase perkecambahan paling sedikit.
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Usami, Yoshihide, Junko Yamaguchi, and Atsushi Numata. "Gliocladins A - C and Glioperazine ; Cytotoxic Dioxo- or Trioxopiperazine Metabolites from a Gliocladium Sp. Separated from a Sea Hare." HETEROCYCLES 63, no. 5 (2004): 1123. http://dx.doi.org/10.3987/com-04-10037.

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Rosales Macias, Diana, José Alfredo Samaniego- Gaxiola, Selenne Yuridia Márquez Guerrero, Arturo Gaytán Mascorro, Yasmín Chew Madinaveitia, and Zaida Crispín del Rio. "Micobiota y Metarhizium asilados de larvas del gusano barrenador del ruezno (Cydia caryana)." Revista Mexicana de Ciencias Agrícolas 8, no. 8 (December 17, 2017): 1785. http://dx.doi.org/10.29312/remexca.v8i8.702.

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La necesidad de contar con organismos nativos con potencial para el control biológico de plagas para el nogal pecanero, motivaron el registro de la micobiota y búsqueda de entomopatógenos en larvas del gusano barrenador del ruezno (GBR) Cydia caryana. Para ello, en el año 2013, en una huerta de nogal en La Laguna México, se recolectaron y procesaron 5 000 rueznos para extraer larvas de GBR, que fueron incubadas en cámara húmeda o suelo. De larvas obtenidas antes del 12 de septiembre y colocadas en suelo se recobró 9% de Fusarium spp. + Gliocladium spp., en contraste, ambos hongos aparecieron 40% en larvas obtenidas después de esa fecha. En larvas obtenidas en diciembre y se colocaron en cámara húmeda, Fusarium + Gliocladium spp. aparecieron 80%. Únicamente fue evidentemente a Metarhizium anisopliae como entomopatógenos de 5% de larvas del GBR colocadas en suelo después del 12 de septiembre, éste hongo también fue entomopatógenos del ácaro rojo gigante Trombidium sp., pulgón negro del nogal Melanocallis caryaefoliae, Garrapata Rhipicephalus sanguineus, y hormiga roja Solenopsis sp. Larvas de GBR pueden usarse como trampa para recobrar Metarhizium y probablemente otros hongos entomopatógenos en suelo de huertas de nogal pecanero.
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Anggraeni, Illa, and Ari Wibowo. "PENGENDALIAN Cylindrocladium sp. PENYEBAB PENYAKIT LODOH PADA BIBIT Acacia mangium Wild. DENGAN FUNGI ANTAGONIS Trichoderma sp. DAN Gliocladium sp." Jurnal Penelitian Hutan Tanaman 6, no. 4 (September 2009): 241–49. http://dx.doi.org/10.20886/jpht.2009.6.4.241-249.

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Stinson, Merritt, David Ezra, Wilford M. Hess, Joe Sears, and Gary Strobel. "An endophytic Gliocladium sp. of Eucryphia cordifolia producing selective volatile antimicrobial compounds." Plant Science 165, no. 4 (October 2003): 913–22. http://dx.doi.org/10.1016/s0168-9452(03)00299-1.

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ISSHIKI, KENGO, MAKI NISHIO, NAOKI SAKURAI, TOMOFUMI UCHIDA, TORU OKUDA, and SABURO KOMATSUBARA. "TMC-52A to D, Novel Cysteine Proteinase Inhibitors, Produced by Gliocladium sp." Journal of Antibiotics 51, no. 7 (1998): 629–34. http://dx.doi.org/10.7164/antibiotics.51.629.

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Yao, Yao, Li Tian, Juan Li, Jiaqing Cao, and Yuehu Pei. "ChemInform Abstract: Cytotoxic Piperazine-2,5-dione Derivatives from Marine Fungus Gliocladium sp." ChemInform 41, no. 1 (January 5, 2010): no. http://dx.doi.org/10.1002/chin.201001218.

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Lestari, Shyntiya Ayu, Umi Kalsum, and Evan Purnama Ramdan. "Efikasi Beberapa Agens Hayati Terhadap Penekanan Pertumbuhan Pyricularia grisea Secara In Vitro." Agrosains : Jurnal Penelitian Agronomi 23, no. 1 (April 1, 2021): 31. http://dx.doi.org/10.20961/agsjpa.v23i1.48174.

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<p>The demand for rice as an important food crop in Indonesia is still constrained by the attack of blast disease caused by <em>Pyricularia grisea</em>. Controlling using synthetic chemical pesticides has a negative impact on the environment so that the use of biological agents is an alternative option. This study aims to determine the effectiveness of several biological agents against the pathogen <em>P. grisea</em> that causes blast disease in rice plants in vitro. The study used a completely randomized design (CRD) with 6 levels of treatment and was repeated 3 times using the dooble culture method. The results of the study showed that the inhibition of <em>P. grisea</em> was the highest in the treatment of <em>P. grisea</em> × <em>Trichoderma</em> sp. and <em>P. grisea</em> × <em>Gliocladium</em> sp. namely 67.04% and 51.85% compared to other treatments. The <em>P. polymyxa</em> and <em>P. fluorescence</em> treatments showed low inhibition, namely 23.70% and 28.89%. Biological agents <em>Trichoderma</em> sp. and <em>Gliocladium</em> sp. able to inhibit the growth of the fungus <em>P. grisea</em>. Each biological agent has a different percentage of inhibition in that caused by the inhibitory mechanism of the biological agent.</p>
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Amalia, Rezeka, Elis Nina Herliyana, and Illa Anggraeni. "POTENSI Trichoderma sp. dan Gliocladium sp. SEBAGAI JAMUR ANTAGONIS TERHADAP Cylindrocladium sp. PENYEBAB PENYAKIT LODOH PADA PERSEMAIAN SECARA IN-VITRO." Jurnal Penelitian Hutan Tanaman 5, no. 1 (May 2008): 63–75. http://dx.doi.org/10.20886/jpht.2008.5.1.63-75.

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Rosa, Lidiya Praktika, Dwi Wahyuni, and Siti Murdiyah. "Isolasi dan Identifikasi Fungi Endofit Tanaman Suruhan (Peperomia pellucida L. Kunth)." Bioma : Berkala Ilmiah Biologi 22, no. 1 (June 27, 2020): 26–45. http://dx.doi.org/10.14710/bioma.22.1.26-45.

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Plants Suruhan (Paperomia pellucida L. Kunth) has traditionally been used as a drug abscesses, boils acne, skin diseases, headaches, relieve pain in arthritic.Various properties owned by the plant Suruhan make this plant as a major commodity medicinal plants are cultivated by the people. Suruhan pharmacological function can not be separated because their secondary metabolites that allegedly resulted from their symbiosis with endophytic microbes such as fungi. Endophytic fungi are microscopic fungi that live intracellularly in the plant tissue without damaging the host. This study aimed to isolate and identify endophytic fungi on plant fruit organs errand. Isolation of endophytic fungi on errands obtained 15 isolates comprising of Fusarium sambucinum, Cladosporium cladosporoides, Cladophialophora sp. Myrothecium sp., Gliocladium sp., Penicillium expansum, Aspergillus sp., Aspergilus niger, Lichtheimia sphaerocystis dan Absidia corymbifera.
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38

Li, Xue-Zheng, Gang Chen, Hai-Feng Wang, Hui-Ming Hua, and Yue-Hu Pei. "Synthesis and bioactivity of diketopiperazine PJ147 and its derivatives from Gliocladium sp. YUP08." Journal of Asian Natural Products Research 16, no. 7 (May 14, 2014): 764–69. http://dx.doi.org/10.1080/10286020.2014.916283.

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39

Shiomi, Kazuro, Noriko Arai, Yuzuru Iwai, Andreas Turberg, Heinz Koelbl, and Satoshi Omura. "ChemInform Abstract: Structure of Argifin, a New Chitinase Inhibitor Produced by Gliocladium sp." ChemInform 31, no. 24 (June 8, 2010): no. http://dx.doi.org/10.1002/chin.200024184.

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40

Zhang, P. G., J. C. Sutton, and A. A. Hopkin. "Evaluation of microorganisms for biocontrol of Botrytiscinerea in container-grown black spruce seedlings." Canadian Journal of Forest Research 24, no. 7 (July 1, 1994): 1312–16. http://dx.doi.org/10.1139/x94-171.

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Microbial isolates from conifer foliage and strawberry were evaluated for biocontrol of Botrytiscinerea Pers.:Fr. in container-grown seedlings of black spruce (Piceamariana (Mill.) B.S.P.). In growth room assays, seedlings were environmentally predisposed to the pathogen and coinoculated with the isolates and B. cinerea. Sporulation incidence of the pathogen was later estimated on needle segments that were cut from the seedlings and incubated on paraquat–chloramphenicol agar medium. The isolates suppressed sporulation by 0–100%. Those of Alternaria, Cladosporium, Epicoccum, yeasts, and bacteria were of low to moderate effectiveness, but those of Gliocladium, Myrothecium, Trichoderma, and Trichothecium were moderately to highly suppressive. In two greenhouse tests, spore suspensions of Gliocladium roseum Link:Bainier and of Myrotheciumverrucaria (Alb. & Schw.) Ditm. ex Stendel. (applied three times) at 2- to 3-week intervals suppressed incidence of B. cinerea by 50–69% and 42–60%, respectively, and were at least as effective as recommended fungicide treatments. Two or three additional applications of the antagonists did not further suppress the pathogen. Isolates of Fusarium sp. and Penicillium sp. were generally ineffective in the greenhouse tests. The four fungi ranked similarly in biocontrol effectiveness in the growth room and greenhouse. It is concluded that the growth room assay provided a strong indication of biocontrol performance in the greenhouse and that G. roseum and M. verrucaria have potential for managing B. cinerea in black spruce in greenhouses.
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Suryaminarsih, Penta, and Tri Mujoko. "Competition of biological agents of Streptomyces sp, Gliocladium sp, and Trichoderma harzianum to Fusarium oxysporum in Tomato Rhizophere." CROPSAVER - Journal of Plant Protection 3, no. 1 (June 30, 2020): 17. http://dx.doi.org/10.24198/cropsaver.v3i1.24173.

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42

Liouane, Kaouthar, Dhouha Saïdana, Hayet Edziri, Samia Ammar, Jihane Chriaa, Mohamed Ali Mahjoub, Khaled Said, and Zine Mighri. "Chemical composition and antimicrobial activity of extracts from Gliocladium sp. growing wild in Tunisia." Medicinal Chemistry Research 19, no. 8 (August 23, 2009): 743–56. http://dx.doi.org/10.1007/s00044-009-9227-3.

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43

ISSHIKI, K., M. NISHIO, N. SAKURAI, T. UCHIDA, T. OKUDA, and S. KOMATSUBARA. "ChemInform Abstract: TMC-52A to D, Novel Cysteine Proteinase Inhibitors, Produced by Gliocladium sp." ChemInform 29, no. 50 (June 18, 2010): no. http://dx.doi.org/10.1002/chin.199850225.

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44

PEIL, GREICE H. S., ANELISE V. KUSS, ANDRÉS F. G. RAVE, JOSÉ P. V. VILLARREAL, YOHANA M. L. HERNANDES, and PATRÍCIA S. NASCENTE. "Bioprospecting of lipolytic microorganisms obtained from industrial effluents." Anais da Academia Brasileira de Ciências 88, no. 3 suppl (August 18, 2016): 1769–79. http://dx.doi.org/10.1590/0001-3765201620150550.

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ABSTRACT The lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. The aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in Pelotas, RS/Brazil. Bacteria were identified using Gram stain and biochemical tests (Vitek 2(r)). Fungi were identified according to macro and micromorphology characteristics. The extracellular lipase production was evaluated using the Rhodamine B test and the enzymatic activity by titration. Twenty-one bacteria were isolated and identified as Klebsiella pneumoniae ssp. pneumoniae, Serratia marcescens, Enterobacter aerogenes, Raoultella ornithinolytica and Raoultella planticola. Were characterized isolated filamentous fungi by the following genera: Alternaria sp., Fusarium sp., Geotrichum sp., Gliocladium sp., Mucor sp., Paecilomyces sp. and Trichoderma sp. Extracellular lipase production was observed in 71.43% of the bacteria and 57.14% of the fungi. The bacterium that presented better promising enzymatic activity was E. aerogenes (1.54 U/ml) however between fungi there was not significant difference between the four isolates. This study indicated that microorganisms lipase producers are present in the industrial effluents, as well as these enzymes have potential of biodegradation of lipid compounds.
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Rybczyńska-Tkaczyk, Kamila, and Teresa Korniłłowicz-Kowalska. "Activities of Versatile Peroxidase in Cultures of Clonostachys rosea f. catenulata and Clonostachys rosea f. rosea during Biotransformation of Alkali Lignin." Journal of AOAC INTERNATIONAL 101, no. 5 (September 1, 2018): 1415–21. http://dx.doi.org/10.5740/jaoacint.18-0058.

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Abstract The aim of this study was the evaluation of activities of versatile peroxidase (VP) in cultures of Clonostachys sp. (Gliocladium sp.) strains during biotransformation of 0.2% alkali lignin (AL). The principal component analysis (PCA) method was applied to determine the main factors and correlation between biotransformation of 0.2% AL, activity of VPs, pH value, and Clonostachys sp. strains. The biotransformation of 0.2% AL in cultures of microscopic fungi included decolorization (medium lightening) and colorization (darkening of the medium). The versatile peroxidase synthesized by the microscopic fungi tested showed activity for oxidation of Mn(II) and guaiacol, but the activity for oxidation of guaiacol in the presence of Mn(II) was significantly higher. The PCA analysis indicated a strong correlation between biotransformation of 0.2% AL and pH and between oxidation of Mn(II), guaiacol without and in the presence of Mn(II) ions, strains, and pH.
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Ela, Nurlela, Lukman Hakim, and Abduh Ulim. "Efektivitas Beberapa Agen Antagonis dan Cara Aplikasinya Untuk Menekan Pertumbuhan Sclerotium rolfsii pada Tanaman Kedelai (Glycine max L. Merrill)." Jurnal Ilmiah Mahasiswa Pertanian 1, no. 1 (November 1, 2016): 155–67. http://dx.doi.org/10.17969/jimfp.v1i1.1012.

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Penelitian ini bertujuan untuk mengetahui efektivitas beberapa agen antagonis serta cara aplikasinya dalam menekan patogen jamur S. rolfsii pada tanaman kedelai. Penelitian menggunakan Rancangan Acak Lengkap (RAL) Faktorial yang terdiri atas 2 faktor. Faktor pertama adalah jenis agen antagonis dan faktor kedua adalah cara aplikasi. Agen antagonis yang dicobakan yaitu Pseudomonas flourescens, Trichoderma sp., Gliocladium sp. dan Coynebacterium sp. diaplikasikan dengan perendaman kemudian diaplikasikan ke dalam tanah. Hasil penelitian menunjukkan bahwa jenis agen antagonis berpengaruh sangat nyata terhadap masa inkubasi post emergence damping-off, jenis agen antagonis berpengaruh nyata terhadap persentase perkecambahan benih kedelai, persentase benih terserang sebelum muncul ke permukaan tanah (pre emergence damping-off), dan persentase benih terserang setelah muncul ke permukaan tanah (post emergence damping-off) namun agen antagonis tidak berpengaruh nyata terhadap tinggi tanaman dan jumlah daun umur 30 hari kemudian cara aplikasi yang paling efektif adalah langsung ke dalam tanah dan tidak terdapat interaksi antara jenis agen antagonis dengan cara aplikasinya.
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47

Sreekanth, D., G. K. Sushim, A. Syed, B. M. Khan, and A. Ahmad. "Molecular and Morphological Characterization of a Taxol-Producing Endophytic Fungus, Gliocladium sp., from Taxus baccata." Mycobiology 39, no. 3 (September 2011): 151–57. http://dx.doi.org/10.5941/myco.2011.39.3.151.

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48

Yulia, Endah, Aldi Rahayu, and Tarkus Suganda. "Antagonisme jamur rizosfer tanaman karet terhadap Rigidoporus microporus secara in vitro dan in planta." Jurnal Agro 9, no. 1 (July 31, 2022): 64–79. http://dx.doi.org/10.15575/17824.

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Penyakit jamur akar putih (JAP) yang disebabkan oleh Rigidoporus microporus merupakan penyakit penting pada tanaman karet. Pengendalian penyakit JAP umumnya menggunakan fungisida sintetik yang berdampak buruk terhadap lingkungan dan berbiaya mahal. Salah satu cara pengendalian penyakit tular tanah yang lebih murah dan efisien adalah pemanfaatan mikroorganisme antagonis. Penelitian ini bertujuan untuk menguji antagonisme jamur rizosfer tanaman karet (JRK) terhadap R. microporus. Penelitian dilaksanakan dari November 2021 hingga Februari 2022 menggunakan metode survei di Perkebunan Karet Rakyat (PKR) Sakambangan, Kabupaten Garut, Jawa Barat serta metode eksperimental di Laboratorium Fitopatologi, Fakultas Pertanian, Universitas Padjadjaran. Rancangan Acak Lengkap digunakan untuk dua uji antagonisme yaitu dual culture (in vitro) berupa perlakuan 17 isolat JRK dan kontrol R. microporus serta uji potongan akar (in planta) berupa perlakuan 8 isolat JRK dan dua kontrol dengan tiga kali ulangan. Dari hasil penelitian diperoleh 17 isolat jamur termasuk genus Trichoderma, Aspergillus, Penicillium, Gliocladium, Paecilomyces, Acremonium dan Cladosporium, serta empat isolat tidak teridentifikasi. Semua isolat menghambat pertumbuhan R. microporus pada uji in vitro dan kolonisasi pada uji in planta dengan penghambatan tertinggi masing-masing 86,07% dan 85,33%. Trichoderma sp., Aspergillus sp. dan Penicillium sp. merupakan jamur antagonis potensial untuk mengendalikan R. microporus asal PKR Sakambangan.ABSTRACTWhite root rot disease (WRRD) incited by Rigidoporus microporus is an important disease in rubber plants. WRRD is commonly controlled using synthetic fungicide, nevertheless it is expensive and harmful to environment. One way to control soil-borne diseases that is considered cheaper, efficient and safer is by using antagonistic microorganisms. This study aimed to examine the antagonism of rubber plant rhizosphere fungi (RRF) against R. microporus. The research was carried out from November 2021 to February 2022. Research used survey method at a rubber plantation in Sakambangan, Garut Regency, West Java, and experimental method at the Phytopathology Laboratory, Faculty of Agriculture, Universitas Padjadjaran. A Completely Randomized Design was used for the two antagonism tests, namely dual culture (in vitro) of 17 RRF isolates and R. microporus as control treatment while a rubber root piece test (in planta) was used for testing 8 RRF isolates and two control treatments with three replications. The results derived 17 fungal isolates in the genera of Trichoderma, Aspergillus, Penicillium, Gliocladium, Paecilomyces, Acremonium, Cladosporium, and four unidentified. All isolates inhibited the growth (86.07%) and colonization (85.33%) of R. microporus. Trichoderma sp., Aspergillus sp. and Penicillium sp. are potential antagonists against R. microporus of Sakambangan rubber plantation origin.
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Davydenko, Kateryna. "Evaluation of fungal endophytes to biological control of Dothistroma needle blight on Pinus nigra subsp. pallasiana (Crimean pine)." Наукові праці Лісівничої академії наук України, no. 19 (December 26, 2019): 22–32. http://dx.doi.org/10.15421/411924.

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Dothistroma needle blight (DNB), caused by Dothistroma septosporum and Dothistroma pini, is the most important forest disease of pine in many countries. This disease has recently emerged in Ukraine as a major threat to mostly Pinus nigra subsp. pallasiana and less to Scots pine. There is increasing evidence that some fungal and bacterial isolates can reduce the growth and pathogenicity of fungal plant pathogens. In this research, infected needles were collected from 30-year-old Crimean pine (P. nigra subsp. pallasiana) in four locations in Southern Ukraine. In total, 244 of endophytic fungi were recovered from needles of Crimean pine during summer sampling of the host’s microbiome in Ukraine in 2012-2014. Dothistroma spp. were detected using fungal isolation and species-specific priming PCR techniques. Among all endophytes, eight fungal species were selected based on the commonness of their occurrence in the foliage of the host and their antagonistic activity. All selected species were tested for their antifungal activity against Dothistroma needle blight. Good antifungal activity against Dothistroma pini was achieved with the Trichoderma sp. and Gliocladium rosea, indicating their good potential possibility in preventing the Dothistroma needle blight on young pines. Moreover, the significant reduction in numbers of conidia and spore germination was found on needles treated with Trichoderma sp. and Gliocladium rosea, compared to conidia numbers following treatment with other fungi. Thus, the use of an effective biological control agent against Dothistroma could be of value in forest nurseries, where it is essential to reduce losses to D. pini infection prior to transferring pines to field sites for planting out.
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Rose, S., M. Parker, and Z. K. Punja. "Efficacy of Biological and Chemical Treatments for Control of Fusarium Root and Stem Rot on Greenhouse Cucumber." Plant Disease 87, no. 12 (December 2003): 1462–70. http://dx.doi.org/10.1094/pdis.2003.87.12.1462.

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Potential disease control methods were evaluated against root and stem rot of cucumber (Cucumis sativus) caused by Fusarium oxysporum f. sp. radicis-cucumerinum. Crab/shrimp shell chitin; three composted media; the biological control agents Pseudomonas chlororaphis strain 63-28, Trichoderma harzianum (RootShield Drench), Streptomyces griseoviridis (Mycostop), Gliocladium catenulatum (Prestop WP, Prestop Mix), and Trichoderma (Gliocladium) virens (SoilGard); and the fungicides thiram or benomyl were added at seeding time followed by inoculation with the pathogen. The addition of chitin (4%, vol/vol) to a peat-based medium significantly (P ≤ 0.05) enhanced seedling growth, increased soil pH, and reduced F. oxysporum f. sp. radicis-cucumerinum populations, but the severity of disease was increased. The addition of composted media (greenhouse compost, windrow composted dairy solids, and vermi-composted dairy solids) to the seeding cavity in a rock wool block medium, followed 48 h later by inoculation with F. oxysporum f. sp. radicis-cucumerinum, reduced seedling mortality when measured after 37 days. Greenhouse compost was significantly (P ≤ 0.05) more suppressive than the other two composts, and the suppression was partially eliminated by sterilization of the compost. The biological control agent G. catenulatum (formulated as Prestop WP and Prestop Mix) significantly reduced seedling mortality when it was applied at seeding 24 h prior to inoculation with the pathogen in the rock wool block medium. None of the other biological control agents reduced disease incidence when compared with control plants under these experimental conditions. Pseudomonas chlororaphis and the fungicide thiram both significantly reduced plant mortality at 17 and 24°C when pathogen-infested seed was treated, or when bacteria-treated and fungicide-treated seed were planted into pathogen-infested peat medium at 24°C. Under semicommercial propagation conditions, treatments consisting of Prestop WP, RootShield Drench, My-costop, and windrow composted dairy solids reduced the severity of disease caused by F. oxysporum f. sp. radicis-cucumerinum in two out of three trials. The efficacy of the biological control agents was affected by seasonal differences in growing conditions, which affected the incidence and severity of the disease. The results from this study indicate that several different approaches can be used at seeding to control Fusarium root and stem rot on greenhouse cucumber.
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