Academic literature on the topic 'Ghost proteins'
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Journal articles on the topic "Ghost proteins":
1
Ali, Randa H., Mohamed E. Ali, and Reham Samir. "Production and Characterization of Bacterial Ghost Vaccine against Neisseria meningitidis." Vaccines 11, no. 1 (December 23, 2022): 37. http://dx.doi.org/10.3390/vaccines11010037.
Abstract:
Bacterial ghosts (BGS) are empty non-living envelopes produced either genetically or chemically. This study investigated a novel chemical protocol for the production of Neisseria meningitidis ghost vaccine using tween 80 followed by a pH reduction with lactic acid. For our vaccine candidate, both safety and immunogenicity aspects were evaluated. The ghost pellets showed no sign of growth upon cultivation. BGS were visualized by scanning electron microscopy, illustrating the formation of trans-membrane tunnels with maintained cell morphology. Gel electrophoresis showed no distinctive bands of the cytoplasmic proteins and DNA, assuring the formation of ghost cells. In animal model, humoral immune response significantly increased when compared to commercial vaccine (p < 0.01). Moreover, serum bactericidal assay (SBA) recorded 94.67% inhibition compared to 64% only for the commercial vaccine after three vaccination doses. In conclusion, this is the first N. meningitidis ghost vaccine candidate, proven to be effective, economic, and with significant humoral response and efficient SBA values; however, clinical studies should be performed.
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Golan, D. E., C. S. Brown, C. M. Cianci, S. T. Furlong, and J. P. Caulfield. "Schistosomula of Schistosoma mansoni use lysophosphatidylcholine to lyse adherent human red blood cells and immobilize red cell membrane components." Journal of Cell Biology 103, no. 3 (September 1, 1986): 819–28. http://dx.doi.org/10.1083/jcb.103.3.819.
Abstract:
Human red blood cells (RBCs) adhere to and are lysed by schistosomula of Schistosoma mansoni. We have investigated the mechanism of RBC lysis by comparing the dynamic properties of transmembrane protein and lipid probes in adherent ghost membranes with those in control RBCs and in RBCs treated with various membrane perturbants. Fluorescence photobleaching recovery was used to measure the lateral mobility of two integral membrane proteins, glycophorin and band 3, and two lipid analogues, fluorescein phosphatidylethanolamine (Fl-PE) and carbocyanine dyes, in RBCs and ghosts adherent to schistosomula. Adherent ghosts manifested 95-100% immobilization of both membrane proteins and 45-55% immobilization of both lipid probes. In separate experiments, diamide-induced cross-linking of RBC cytoskeletal proteins slowed transmembrane protein diffusion by 30-40%, without affecting either transmembrane protein fractional mobility or lipid probe lateral mobility. Wheat germ agglutinin- and polylysine-induced cross-linking of glycophorin at the extracellular surface caused 80-95% immobilization of the transmembrane proteins, without affecting the fractional mobility of the lipid probe. Egg lysophosphatidylcholine (lysoPC) induced both lysis of RBCs and a concentration-dependent decrease in the lateral mobility of glycophorin, band 3, and Fl-PE in ghost membranes. At a concentration of 8.4 micrograms/ml, lysoPC caused a pattern of protein and lipid immobilization in RBC ghosts identical to that in ghosts adherent to schistosomula. Schistosomula incubated with labeled palmitate released lysoPC into the culture medium at a rate of 1.5 fmol/h per 10(3) organisms. These data suggest that lysoPC is transferred from schistosomula to adherent RBCs, causing their lysis.
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Jawale, Chetan V., Nithiphonh Somsanith, Seong Kug Eo, Sang-Youel Park, and John Hwa Lee. "Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid." Acta Veterinaria Hungarica 63, no. 4 (December 2015): 401–12. http://dx.doi.org/10.1556/004.2015.038.
Abstract:
Escherichia coli heat-labile enterotoxin B subunit (LTB) protein is a potent adjuvant. Salmonella Gallinarum ghosts carrying LTB (S. Gallinarum-LTB ghosts) were genetically constructed using a plasmid, pJHL187-LTB, designed for the co-expression of the LTB and E lysis proteins. This study evaluates the immunopotentiating effects of Montanide™ ISA 70 VG on S. Gallinarum-LTB ghost vaccination against fowl typhoid. Five-week-old layer chickens were injected intramuscularly with sterile PBS (non-immunised control, Group A), S. Gallinarum-LTB ghost (Group B) or S. Gallinarum-LTB ghost emulsified with Montanide™ ISA 70 VG adjuvant (Group C). Chickens from both Groups B and C showed significant induction of antigen-specific systemic IgG response compared to controls; in addition, Group C showed enhanced induction of systemic IgG response compared to Group B. We observed significant induction of antigen-specific lymphocyte proliferative response and increased mRNA levels of Th1 cytokines (IFN-γ and IL2) in both Groups B and C. Furthermore, in the challenge experiment with a virulent strain of S. Gallinarum, Group C showed higher survival rates compared with other groups. These results indicate that vaccination with the S. Gallinarum-LTB ghost in combination with Montanide™ ISA 70 VG may enhance the protective immunity against fowl typhoid.
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Wooden, Jason M., Greg Finney, Michael MacCoss, and Diana M. Gilligan. "Comprehensive Analysis of Murine and Human RBC Ghost Proteomes." Blood 108, no. 11 (November 16, 2006): 1569. http://dx.doi.org/10.1182/blood.v108.11.1569.1569.
Abstract:
Abstract Inherited hemolytic anemia (spherocytosis or elliptocytosis) is one of the most common inherited diseases with an incidence of 1:2500 to 1:5000 in populations of Northern European descent. Mild to severe inherited hemolytic anemias can arise from defects in the red blood cell (RBC) membrane skeleton. Genetic knock-out of various components of this apparatus has led to the creation of mouse models which have contributed significantly to our understanding of these disorders in humans. However, the mouse and human RBC protein complements have not been comprehensively compared. Using newly developed proteomic methodology, we conducted a peptide level ‘bottom-up’ analysis of the normal mouse and human RBC ghost (i.e., RBC membrane skeleton and associated proteins). RBCs were purified using cellulose acetate chromatography from whole blood taken from three genetically identical mice and two hematologically normal yet genetically diverse humans. The isolated RBCs were lysed to generate RBC ghosts whose protein complements were digested with trypsin and analyzed by shotgun proteomics using microcapillary liquid chromatography coupled with tandem mass spectrometry. In total, 400 and 491 unique proteins were identified in human samples A and B respectively while 469 proteins were found in common across the three mouse samples. All previously identified membrane skeleton proteins were found in the human and mouse samples. Likewise, well-known RBC membrane proteins were represented. Of interest, a surprising number of proteins were found associated with the RBC ghost involved in processes such as protein repair (15–20), protein degradation (30–43), oxidative stress response (4–6), Ras oncogene biology (28–30), and glycolysis (3–6). Collectively, the two human samples represented 544 unique proteins. These results affirm the usefulness of inherited anemia mouse models given the observed conservation of membrane skeleton components and the inherent challenges with doing normal versus diseased RBC analysis in humans due to genetic variation.
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Nunes-Correia, Isabel, João Ramalho-Santos, and Maria C. Pedroso de Lima. "Sendai Virus Fusion Activity as Modulated by Target Membrane Components." Bioscience Reports 18, no. 2 (April 1, 1998): 59–68. http://dx.doi.org/10.1023/a:1020180109275.
Abstract:
We have studied the differences between erythrocytes and erythrocyte ghosts as target membranes for the study of Sendai virus fusion activity. Fusion was monitored continuously by fluorescence dequenching of R18-labeled virus. Experiments were carried out either with or without virus/target membrane prebinding. When Sendai virus was added directly to a erythrocyte/erythrocyte ghost suspension, fusion was always lower than that obtained when experiments were carried out with virus already bound to the erythrocyte/erythrocyte ghost in the cold, since with virus prebinding fusion can be triggered more rapidly. Although virus binding to both erythrocytes and erythrocyte ghosts was similar, fusion activity was much more pronounced when erythrocyte ghosts were used as target membranes. These observations indicate that intact erythrocytes and erythrocyte ghosts are not equivalent as target membranes for the study of Sendai virus fusion activity. Fusion of Sendai virus with both target membranes was inhibited when erythrocytes or erythrocyte ghosts were pretreated with proteinase K, suggesting a role of target membrane proteins in this process. Treatment of both target membranes with neuraminidase, which removes sialic acid residues (the biological receptors for Sendai virus) greatly reduced viral binding. Interestingly, this treatment had no significant effect on the fusion reaction itself.
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Hopgood, M. F., S. E. Knowles, and F. J. Ballard. "Proteolysis of N-ethylmaleimide-modified aldolase loaded into erythrocyte ghosts: prevention by inhibitors of calpain." Biochemical Journal 259, no. 1 (April 1, 1989): 237–42. http://dx.doi.org/10.1042/bj2590237.
Abstract:
1. When rabbit muscle aldolase labelled with tritium and inactivated by N-ethylmaleimide (NEM) was loaded into erythrocyte ghosts, significant proteolysis of the loaded protein occurred. The major product of this proteolysis, separated by electrophoresis under dissociating conditions, was found to be approx. 2 kDa smaller than the parent protein. 2. Proteolysis was detectable during erythrocyte ghost loading at 0 degrees C, reaching a plateau after approx. 12 min. Subsequent incubation at 37 degrees C to allow resealing of the ghosts resulted in additional proteolysis, and up to 20% of the loaded protein was converted to the smaller 38 kDa derivative. 3. EDTA, EGTA, leupeptin and chymostatin, each inhibitors of calcium-activated neutral proteinases (calpains), were the most effective inhibitors of the proteolysis of NEM-inactivated aldolase in ghosts. Other proteinase inhibitors were ineffective, while phenylmethanesulphonyl fluoride was only partially effective. 4. Inhibition of the proteolysis by EGTA was prevented by CaCl2, supporting the involvement of erythrocyte calpain. 5. Pretreatment of ghosts with EGTA prior to loading of NEM-modified aldolase followed by microinjection of the protein into HeLa cells did not result in a different rate of its overall breakdown to acid-soluble products. EGTA is suggested as a useful agent for the erythrocyte ghost-mediated microinjection of calpain-sensitive proteins.
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Yang, Chinglai, Qingyuan Yang, and Richard W. Compans. "Coreceptor-Dependent Inhibition of the Cell Fusion Activity of Simian Immunodeficiency Virus Env Proteins." Journal of Virology 74, no. 13 (July 1, 2000): 6217–22. http://dx.doi.org/10.1128/jvi.74.13.6217-6222.2000.
Abstract:
ABSTRACT The cytoplasmic tail (R peptide) sequence is able to regulate the fusion activity of the murine leukemia virus (MuLV) envelope (Env) protein. We have previously shown that this sequence exerts a profound inhibitory effect on the fusion activity of simian immunodeficiency virus (SIV)-MuLV chimeric Env proteins which contain the extracellular and transmembrane domains of the SIV Env protein. Recent studies have shown that SIV can utilize several alternative cellular coreceptors for its fusion and entry into the cell. We have investigated the fusion activity of SIV and SIV-MuLV chimeric Env proteins using cells that express different coreceptors. HeLa cells were transfected with plasmid constructs that carry the SIV or SIV-MuLV chimeric Env protein genes and were overlaid with either CEMx174 cells or Ghost Gpr15 cells, which express the Gpr15 coreceptor for SIV, or Ghost CCR5 cells, which express CCR5, an alternate coreceptor for SIV. The R-peptide sequence in the SIV-MuLV chimeric proteins was found to inhibit the fusion with CEMx174 cells or Ghost Gpr15 cells. However, a significant level of fusion was still observed when HeLa cells expressing the chimeric Env proteins were cocultivated with Ghost CCR5 cells. These results show that the R-peptide sequence exerts differential effects on the fusion activity of SIV Env proteins using target cells that express alternative coreceptors.
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Turrini, F., A. Naitana, L. Mannuzzu, G. Pescarmona, and P. Arese. "Increased red cell calcium, decreased calcium adenosine triphosphatase, and altered membrane proteins during fava bean hemolysis in glucose-6- phosphate dehydrogenase-deficient (Mediterranean variant) individuals." Blood 66, no. 2 (August 1, 1985): 302–5. http://dx.doi.org/10.1182/blood.v66.2.302.302.
Abstract:
Abstract RBCs from four glucose-6-phosphate dehydrogenase (G6PD)-deficient (Mediterranean variant) subjects were studied during fava bean hemolysis. In the density-fractionated RBC calcium level, Ca2+-ATPase activity, reduced glutathione level, and ghost protein pattern were studied. In the bottom fraction, containing most heavily damaged RBCs, calcium level ranged from 143 to 244 mumol/L RBCs (healthy G6PD- deficient controls: 17 +/- 5 mumol/L RBCs). The Ca2+-ATPase activity ranged from 0.87 to 1.84 mumol ATP consumed/g Hb/min (healthy G6PD- deficient controls: 2.27 +/- 0.4). Sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) of ghosts showed: (1) the presence of high mol wt aggregates (in three cases they were reduced by dithioerythritol; in one case, only partial reduction was possible); (2) the presence of multiple, scattered new bands; and (3) the reduction of band 3. Oxidant-mediated damage to active calcium extrusion, hypothetically associated with increased calcium permeability, may explain the large increase in calcium levels. They, in turn, could activate calcium-dependent protease activity, giving rise to the profound changes in the ghost protein pattern.
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Turrini, F., A. Naitana, L. Mannuzzu, G. Pescarmona, and P. Arese. "Increased red cell calcium, decreased calcium adenosine triphosphatase, and altered membrane proteins during fava bean hemolysis in glucose-6- phosphate dehydrogenase-deficient (Mediterranean variant) individuals." Blood 66, no. 2 (August 1, 1985): 302–5. http://dx.doi.org/10.1182/blood.v66.2.302.bloodjournal662302.
Abstract:
RBCs from four glucose-6-phosphate dehydrogenase (G6PD)-deficient (Mediterranean variant) subjects were studied during fava bean hemolysis. In the density-fractionated RBC calcium level, Ca2+-ATPase activity, reduced glutathione level, and ghost protein pattern were studied. In the bottom fraction, containing most heavily damaged RBCs, calcium level ranged from 143 to 244 mumol/L RBCs (healthy G6PD- deficient controls: 17 +/- 5 mumol/L RBCs). The Ca2+-ATPase activity ranged from 0.87 to 1.84 mumol ATP consumed/g Hb/min (healthy G6PD- deficient controls: 2.27 +/- 0.4). Sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) of ghosts showed: (1) the presence of high mol wt aggregates (in three cases they were reduced by dithioerythritol; in one case, only partial reduction was possible); (2) the presence of multiple, scattered new bands; and (3) the reduction of band 3. Oxidant-mediated damage to active calcium extrusion, hypothetically associated with increased calcium permeability, may explain the large increase in calcium levels. They, in turn, could activate calcium-dependent protease activity, giving rise to the profound changes in the ghost protein pattern.
10
Cardon, Tristan, Isabelle Fournier, and Michel Salzet. "SARS-Cov-2 Interactome with Human Ghost Proteome: A Neglected World Encompassing a Wealth of Biological Data." Microorganisms 8, no. 12 (December 19, 2020): 2036. http://dx.doi.org/10.3390/microorganisms8122036.
Abstract:
Conventionally, eukaryotic mRNAs were thought to be monocistronic, leading to the translation of a single protein. However, large-scale proteomics have led to a massive identification of proteins translated from mRNAs of alternative ORF (AltORFs), in addition to the predicted proteins issued from the reference ORF or from ncRNAs. These alternative proteins (AltProts) are not represented in the conventional protein databases and this “ghost proteome” was not considered until recently. Some of these proteins are functional and there is growing evidence that they are involved in central functions in physiological and physiopathological context. Based on our experience with AltProts, we were interested in finding out their interaction with the viral protein coming from the SARS-CoV-2 virus, responsible for the 2020 COVID-19 outbreak. Thus, we have scrutinized the recently published data by Krogan and coworkers (2020) on the SARS-CoV-2 interactome with host cells by affinity purification in co-immunoprecipitation (co-IP) in the perspective of drug repurposing. The initial work revealed the interaction between 332 human cellular reference proteins (RefProts) with the 27 viral proteins. Re-interrogation of this data using 23 viral targets and including AltProts, followed by enrichment of the interaction networks, leads to identify 218 RefProts (in common to initial study), plus 56 AltProts involved in 93 interactions. This demonstrates the necessity to take into account the ghost proteome for discovering new therapeutic targets, and establish new therapeutic strategies. Missing the ghost proteome in the drug metabolism and pharmacokinetic (DMPK) drug development pipeline will certainly be a major limitation to the establishment of efficient therapies.
Dissertations / Theses on the topic "Ghost proteins":
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Garcia, del Rio Diego Fernando. "Studying protein complexes for assessing the function of ghost proteins (Ghost in the Cell)." Electronic Thesis or Diss., Université de Lille (2022-....), 2023. https://pepite-depot.univ-lille.fr/ToutIDP/EDBSL/2023/2023ULILS115.pdf.
Abstract:
Le cancer de l'ovaire (OvCa) est le cancer le plus mortel parmi les cancers féminins. Il est souvent diagnostiqué tardivement ou mal diagnostiqué, ce qui le rend difficile à traiter. Les options de traitement incluent la chirurgie ou la chimiothérapie, toutefois la résistance à la chimiothérapie est un problème majeur. Il est donc urgent de trouver de nouvelles cibles et de développer de nouvelles stratégies pour surmonter cette résistance.Dans ce contexte le protéome fantôme est une source potentiellement riche de biomarqueurs. Le protéome fantôme, ou protéome alternatif, est composé de protéines traduites à partir de cadres de lecture ouverts alternatifs (AltORFs). Ces AltORFs proviennent de différents codons START issus de différente région de l'ARNm, tels qu'un décalage de cadre de lecture (+1, +2) dans la séquence codante de l'ADN (CDS), dans le 5'-UTR, 3'-UTR et éventuellement de la traduction d'ARN non codants (ncRNA).Les études sur les protéines alternatives (AltProts) sont souvent complexes et nécessite des études biomoléculaires coûteuses. Cependant, leurs fonctions peuvent être déduites en identifiant leurs partenaires d'interaction, la détection des interactions protéine-protéine (PPI) entre AltProts et protéines de référence (RefProts) peut aider à identifier leur fonction. La stratégie de pontage chimique (crosslink) combiné à la spectrométrie de masse (XL-MS) est un outil approprié à cet objectif. De plus, les outils bioinformatiques qui relient les informations fonctionnelles des RefProt et les analyses d'ontologie génique (GO) permettent la visualisation des voies de signalisation et le regroupement des RefProts en fonction de leur processus biologique, de leur fonction moléculaire ou de leur localisation cellulaire, et ainsi y placer certaine AltProt.Dans ce travail, nous avons développé une méthodologie combinant XL-MS et le fractionnement subcellulaire. L'étape de fractionnement subcellulaire nous a permis de réduire la complexité des échantillons analysés par chromatographie liquide et spectrométrie de masse (LC-HRMS/MS). Pour évaluer la validité des interactions, nous avons réalisé une modélisation moléculaire des structures 3D des AltProts, suivie d'une prédiction informatique de l'interaction et de mesure des distances de pontages identifiés expérimentalement. L'analyse a révélé des rôles d'AltProts dans les fonctions et les processus biologiques tel que la réparation de l'ADN ou encore la présentation d'antigène.La protéogénomique a été utilisée pour générer des bases de données protéiques personnalisées à partir des données de séquençage ARN afin d'étudier les protéomes de deux lignées cellulaires de cancer de l'ovaire (PEO-4 et SKOV-3) en comparaison avec une lignée cellulaire ovarienne normale (T1074). L'expression différentielle de plusieurs protéines a ainsi été identifiée entre les lignées cellulaires cancéreuses et normales, avec une association aux voies de signalisation connues pour le cancer. Des PPI ont également été identifiées dans les lignées cellulaires cancéreuses en utilisant la méthodologie XL-MS.Ce travail met en évidence le potentiel de l'approche protéogénomique pour découvrir de nouveaux aspects de la biologie du cancer de l'ovaire. Il nous permet d'identifier des protéines et des variants auparavant inconnus qui peuvent avoir une signification fonctionnelle. L'utilisation de bases de données protéiques personnalisées et de l'approche de réticulation a mis en lumière le "protéome fantôme", une vision du protéome restée inexplorée jusqu'à présentOvarian cancer (OvCa) has the highest mortality rate among female reproductive cancers worldwide. OvCa is often referred to as a stealth killer because it is commonly diagnosed late or misdiagnosed. Once diagnosed, OvCa treatment options include surgery or chemotherapy. However, chemotherapy resistance is a significant obstacle. Therefore, there is an urgent need to identify new targets and develop novel therapeutic strategies to overcome therapy resistance.In this context the ghost proteome is a potentially rich source of biomarkers. The ghost proteome, also known as the alternative proteome, consists of proteins translated from alternative open reading frames (AltORFs). These AltORFs originate from different start codons within mRNA molecules, such as the coding DNA sequence (CDS) in frameshifts (+1, +2), the 5'-UTR, 3'-UTR, and possible translation products from non-coding RNAs (ncRNA).Studies on alternative proteins (AltProts) are often limited due to their case-by-case occurrence and complexity. Obtaining functional protein information for AltProts requires complex and costly biomolecular studies. However, their functions can be inferred by profiling their interaction partners, known as "guilty by association" approaches. Indeed, assessing AltProts' protein-protein interactions (PPIs) with reference proteins (RefProts) can help identify their function and set them as research targets. Since there is a lack of antibodies against AltProts, crosslinking mass spectrometry (XL-MS) is an appropriate tool for this task. Additionally, bioinformatic tools that link protein functional information through networks and gene ontology (GO) analysis are also powerful. These tools enable the visualization of signaling pathways and the grouping of RefProts based on their biological process, molecular function, or cellular localization, thus enhancing our understanding of cellular mechanisms.In this work, we developed a methodology that combines XL-MS and subcellular fractionation. The key step of subcellular fractionation allowed us to reduce the complexity of the samples analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). To assess the validity of crosslinked interactions, we performed molecular modeling of the 3D structures of the AltProts, followed by docking studies and measurement of the corresponding crosslink distances. Network analysis indicated potential roles for AltProts in biological functions and processes. The advantages of this workflow include non-targeted AltProt identification and subcellular identification.Additionally, a proteogenomic analysis was performed to investigate the proteomes of two ovarian cancer cell lines (PEO-4 and SKOV-3 cells) in comparison to a normal ovarian epithelial cell line (T1074 cell). Using RNA-seq data, customized protein databases for each cell line were generated. Differential expression of several proteins, including AltProts, was identified between the cancer and normal cell lines. The expression of some RefProts and their transcripts were associated with cancer-related pathways. Moreover, the XL-MS methodology described above was used to identify PPIs in the cancerous cell lines.This work highlights the significant potential of proteogenomics in uncovering new aspects of ovarian cancer biology. It enables us to identify previously unknown proteins and variants that may have functional significance. The use of customized protein databases and the crosslinking approach have shed light on the "ghost proteome," an area that has remained unexplored until now
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Lee, Sang 1972. "Molecular analysis of the DlgPSD-95 family of membrane-associated guanylate kinases in the weakly electric fish, Apteronotus leptorhynchus." Thesis, McGill University, 1999. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=29906.
Abstract:
Members of the Dlg/PSD-95 protein family have a modular organization with multiple protein interaction domains and are thought to be important in the organization of synapses. A degenerate primer PCR strategy was used to screen for members of this family in the central nervous system of Apteronotus leptorhynchus, which possesses an electrosensory system that is well-suited for the study of synaptic organization and the subcellular localization of proteins. A. leptorhynchus was found to express at least four Dlg/PSD-95 family genes as in mammals, and the full coding sequences of the homologues of mammalian PSD-95 and SAP102 were determined. In situ hybridization experiments performed for the A. leptorhynchus Dig/PSD-95 family members in brain showed that they have a differential pattern of expression.
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Ghosh, Subhajit [Verfasser], and Markus [Akademischer Betreuer] Hecker. "Role of the focal adhesion protein zyxin in hypertension-induced cardiovascular remodelling / Subhajit Ghosh ; Betreuer: Markus Hecker." Heidelberg : Universitätsbibliothek Heidelberg, 2015. http://d-nb.info/1180301854/34.
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Ghosh, Sanchita [Verfasser], Andreas Gutachter] Müller, and Triantafyllos [Gutachter] [Chavakis. "The regulation of endoplasmic reticulum stress by activated protein C in diabetic nephropathy / Sanchita Ghosh ; Gutachter: Andreas Müller, Triantafyllos Chavakis." Magdeburg : Universitätsbibliothek Otto-von-Guericke-Universität, 2019. http://d-nb.info/1219966347/34.
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Ghosh, Sanchita [Verfasser], Andreas [Gutachter] Müller, and Triantafyllos [Gutachter] Chavakis. "The regulation of endoplasmic reticulum stress by activated protein C in diabetic nephropathy / Sanchita Ghosh ; Gutachter: Andreas Müller, Triantafyllos Chavakis." Magdeburg : Universitätsbibliothek Otto-von-Guericke-Universität, 2019. http://d-nb.info/1219966347/34.
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Ghosh, Madhumita [Verfasser]. "Structural and biochemical characterization of proteins involved in cancer / Madhumita Ghosh." 2005. http://d-nb.info/974284823/34.
Books on the topic "Ghost proteins":
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Graber, Jennifer. 1893 to 1903. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780190279615.003.0008.
Abstract:
With allotment legislation passed, American Protestants vigorously campaigned for the dissolution of reservations and tribal governments. They also amplified their efforts to “civilize” Native people. They sent numerous missionaries to Indian Country. They argued for increased funding for Indian education, especially off-reservation boarding schools that separated Native children from their families. Kiowas struggled to hold on to their lands and maintain communal ties in an increasingly difficult environment. No longer practicing one of their central communal rites, Kiowas turned to other sources of sacred power for healing and protection. Some participated in peyote rites, others affiliated with churches, and still others reinvigorated the Ghost Dance. Even as they tried numerous ritual options, Kiowas worked to delay allotment. Some took their protests all the way to the Supreme Court, which heard one of the most important Indian land cases in American history, Lonewolf v. Hitchcock, in 1903.
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Comics, Gwandanaland. Complete Ghost Rider : Volume 1 : Gwandanaland Comics #114 -- the Original Golden Age Ghost Rider! He Protects the West from Outlaws, Living and Unliving! This Book: From Issues #1-14. Independently Published, 2019.
Book chapters on the topic "Ghost proteins":
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Bojesen, Inge N., and Eigil Bojesen. "Fatty acid-binding to erythrocyte ghost membranes and transmembrane movement." In Cellular Fatty Acid-binding Proteins, 209–15. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4615-3936-0_26.
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Boulanger, Pascale. "Purification of Bacteriophages and SDS-PAGE Analysis of Phage Structural Proteins from Ghost Particles." In Methods in Molecular Biology, 227–38. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-565-1_13.
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Delvecchio, Vito G., Tim Alefantis, Rodolfo A. Ugalde, Diego Comerci, Maria Ines Marchesini, Akbar Khan, Werner Lubitz, and Cesar V. Mujer. "Identification of Protein Candidates for Developing Bacterial Ghost Vaccines againstBrucella." In Methods of Biochemical Analysis, 363–77. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2005. http://dx.doi.org/10.1002/0471973165.ch19.
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Mordden, Ethan. "Dancing With a Ghost." In Pick a Pocket Or Two, 73–89. Oxford University Press, 2021. http://dx.doi.org/10.1093/oso/9780190877958.003.0006.
Abstract:
This chapter evaluates British musicals in the 1930s and 1940s. This period, the chapter argues, was characterized by a lack of ambition. One problem was the paucity of Jewish writers in the UK. This ethnic group is notable for imaginative expertise in musical theatre and this is one of many reasons why the American brand is so protean. The most disappointing element could be argued to be passéiste choreography. In the meantime, the American musical was letting dance evolve most originally than in the UK. It is worth noting that Charles B. Cochran hired American choreographers for his most aspiring shows of the early 1930s, Ever Green (1930) and Nymph Errant (1933). Ever Green is a spectacular book musical that introduced Britain to the recent German invention of the revolving stage. The comedy musical then became less popular. However, Me and My Girl (1937) was an outstanding comedy musical. Me and My Girl was the work of composer Noel Gay, with L. Arthur Rose and Douglas Furber involved. Though as producer-director Lupino Lane could be called the show's auteur.
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Ng, Jenna. "Holograms/Holographic Projections : Ghosts Amongst the Living; Ghosts of the Living." In The Post-Screen Through Virtual Reality, Holograms and Light Projections. Nieuwe Prinsengracht 89 1018 VR Amsterdam Nederland: Amsterdam University Press, 2021. http://dx.doi.org/10.5117/9789463723541_ch04.
Abstract:
This chapter explicates holographic projections as the second instantiation of post-screen media. Often mistaken as holograms, these projections of images ranging from Tupac to Julian Assange to holographic protests redraw the boundaries between life and death, and enable a re-imagination of ghosts, deadness, aliveness and afterlife. The chapter argues for four different moments in a history of ghosts in the media: resurrection; necrophilia; necromancy; and interactivity. The last facilitates spectral life in the post-screen through considering holographic projections of both dead and living figures. In relation to the dead, the post-screen becomes a space in limbo between deadness and aliveness; in relation to the living, the realness of the holographic body stretches in a tetravalence across dual axes of actual/virtual and here/elsewhere, and enlivened in what I call vivification. In these 3D displays on the post-screen of resurrected and vivified bodies, different kinds of life, afterlife and after-death emerge.
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Koch, Christof. "Diffusion, Buffering, and Binding." In Biophysics of Computation. Oxford University Press, 1998. http://dx.doi.org/10.1093/oso/9780195104912.003.0017.
Abstract:
In Chap. 9 we introduced calcium ions and alluded to their crucial role in regulating the day-to-day life of neurons. The dynamics of the free intracellular calcium is controlled by a number of physical and chemical processes, foremost among them diffusion and binding to a host of different proteins, which serve as calcium buffers and as calcium sensors or triggers. Whereas buffers simply bind Ca2+ above some critical concentration, releasing it back into the cytoplasm when [Ca2+]i has been reduced below this level, certain proteins— such as calmodulin—change their conformation when they bind with Ca2+ ions, thereby activating or modulating enzymes, ionic channels, or other proteins. The calcium concentration inside the cell not only determines the degree of activation of calcium-dependent potassium currents but—much more importantly—is relevant for determining the changes in structure expressed in synaptic plasticity. As discussed in Chap. 13, it is these changes that are thought to underlie learning. Given the relevance of second messenger molecules, such as Ca2+, IP3, cyclic AMP and others, for the processes underlying growth, sensory adaptation, and the establishment and maintenance of synaptic plasticity, it is crucial that we have some understanding of the role that diffusion and chemical kinetics play in governing the behavior of these substances. Today, we have unprecedented access to the spatio-temporal dynamics of intracellular calcium in individual neurons using fluorescent calcium dyes, such as fura-2 or fluo-3, in combination with confocal or two-photon microscopy in the visible or in the infrared spectrum (Tsien, 1988; Tank et al., 1988; Hernández-Cruz, Sala, and Adams, 1990; Ghosh and Greenberg, 1995).
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Haughton, Hugh. "Tradition and Phantasmagoria." In The Oxford Handbook of W.B. Yeats, 167—C12N74. Oxford University Press, 2023. http://dx.doi.org/10.1093/oxfordhb/9780198834670.013.18.
Abstract:
Abstract In his essay on ‘Dante’, T. S. Eliot claimed magisterially that ‘Dante and Shakespeare divide the modern world between them. There is no third.’ On this reading, if we discount the classical poets, Dante and Shakespeare are the most charged avatars of poetic tradition. This was equally true for W. B. Yeats, a poet with a comparable sense of spiritual belatedness and whose Irish background put him at a fraught angle to European tradition. Though Yeats generally undervalues Shakespearean comedy, the plausibility of the ‘gay’ knowledge he ascribes to Hamlet and Lear partly depends on Shakespeare’s protean commitment to ‘antic’ wit. Dante is a less palpable linguistic presence than Shakespeare in late Yeats, despite the Irish poet’s comparable commitment to a Commedia-like poetry and drama of the afterlife. Nevertheless, the ghost of Dantean verse haunts late poems like ‘Cuchulain Comforted’, written a couple of weeks before the poet’s death.
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Gallo, Giuseppe. "Reducing Compounds Roles in Oxidative Stress Relieving of Human Red Blood Cells." In Reactive Oxygen Species [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.99977.
Abstract:
Oxidative stress is the consequence of an imbalance between pro-oxidant and antioxidant processes. Antioxidants that counteract reactive oxygen species do not all work the same way. Both resveratrol and the more powerful 4-hydroxytyrosol are excellent reducing agents. Polyphenol treatment (red wine polyphenols, resveratrol and catechin) is associated with a significant increase in anion permeability for chloride compared with control and 2.2′-azobis-2 amidinopropan dihydrochloride affected cells. Treatment with polyphenols was associated with a significant reduction in mean ± standard error of the mean membrane lipid peroxidation compared with control and 2.2′-azobis-2 amidinopropan dihydrochloride treatment. Hemolysis data are also obtained in the previously described conditions. 4-hydroxytyrosol is shown to significantly protect red blood cells from oxidative damage by 4-hydroxynonenal. But there are paradoxical effects like uric acid and creatinine. The obtained data evidence that both creatinine and uric acid levels have influence on the ratio of both malondialdehyde/protein and 4-hydroxynonenal/protein content on red blood cell ghosts, demonstrating their possible protective role against oxidative stress at low concentrations in blood and oxidizing power at higher concentrations. Finally, polyunsaturated fatty acids do not have all this reducing power.
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Spoo, Robert. "Introduction." In James Joyce and the Language of History, 3–13. Oxford University PressNew York, NY, 1994. http://dx.doi.org/10.1093/oso/9780195087499.003.0001.
Abstract:
Abstract The word “history” reverberates throughout Ulysses like the laugh ofa ghost. Fiercely contested and continually appropriated, it can probably lay claim to more transformations than the protean dog Stephen Dedalus watches on Sandymount Strand. The Englishman Haines observes with imperial serenity that “it seems history is to blame” for his nation’s treatment of Ireland (1.649), while Stephen complains that “history ... is a nightmare from which I am trying to awake” (2.377). Mr. Deasy, the prating, imperturbable Orangeman, affirms that history moves “towards one great goal, the manifestation of God” (2.381), but the outsider Leopold Bloom, bearding the superpatriotic Citizen in his gloryhole, offers a less sanguine opinion: “Persecution ... all the history of the world is full ofit Force, hatred, history, all that. That’s not life for men and women, insult and hatred” (12.1417, 1481-82). A provocatively complex word whenever Joyce uses it, “history” carries an especially heavy, shifting freight of meaning in Ulysses that makes it the verbal counterpart of Stephen, who personally labors under the burden of the past.
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Spoo, Robert. "Fabricated Ghosts: A Metahistorical Reading of A Portrait of the Artist as a Young Man." In James Joyce and the Language of History, 38–65. Oxford University PressNew York, NY, 1994. http://dx.doi.org/10.1093/oso/9780195087499.003.0003.
Abstract:
Abstract Joyce had the inspiration for a short story called “Ulysses” in Rome in 1906 but was unable to get “any forrader than the title,” as he explained to his brother (Letters 11209). After returning to Trieste, he finished “The Dead,” another story he had conceived in Rome, in September 1907, and quickly set about rewriting the sprawling manuscript of Stephen Hero in the condensed five-chapter format he was to call A Portrait of the Artist asa Young Man. After many frustrations and interruptions he finished the novel in 1913-14.1 “Ulysses,” and the experiences in Trieste and Rome that had prepared Joyce for writing it, had to be set aside while the long-gestating bildungsroman struggled toward completion, a work whose content, though not its form, had taken conceptual shape by 1904. While writing Ulysses, Joyce told his friend Frank Budgen that he was losing interest in Stephen Dedalus because the young man, in contrast to the protean Leopold Bloom and the expanding text of Ulysses itself, had acquired a shape that could not be changed.2 But Stephen may have begun to be a burden to Joyce almost as early as 1904, when he embarked on his autobiographical novel; ousting all other creative and historical possibilities, Stephen became part ofJ oyce ‘s personal nightmare of history, a juvenile alter ego persisting as a negative afterimage, a goad to writing that was also a clog on experimental impulse.
Conference papers on the topic "Ghost proteins":
1
FACON, T., J. GOUPEMAND, C. CARON, M. ZANDECKI, M. H. ESTIENNE, and A. COSSON. "GRAY PLATELET SYNDROME AND IDIOPATHIC PULMONARY FIBROSIS OCCURRING IN THE SAME PATIENT : A FORTUITOUS ASSOCIATION?" In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644559.
Abstract:
A 46 yr old Caucasian woman has been diagnosed as having a congenital deficiency of platelet a-granules (gray platelet syndrome - GPS) associated with an extensive idiopathic pulmonary fibrosis (IPF). The patient had a life long history of bleeding tendency including dental bleedings in childhood, intraperitoneal bleeding, metrorrhagias which led to hysterectomy, and post-operative hemorrhages. When aged 16, splenectomy was performed because of a mild thrombocytopenia but did not result in a subsequent improvement of the platelet count. The spleen was enlarged and showed an excess of fibrous tissue.Evaluation of hemostasis (July 1986) revealed a moderate thrombocytopenia of 120 × 109/1 constrasting with a markedly prolonged Simplate bleeding time (>30 min.). When examined on stained blood films, the platelets presented a "ghost-like" gray appearance. The mean platelet volume (coulter S + IV) was increased to 14 μ3 (N : 6.5-9.5 μ3). Ultrastructural studies confirmed the lack of a-granules and showed normal presence of dense-bodies, mitochondria and peroxisomes. Platelet aggregation was decreased when induced by thrombin, ADP and collagen but normal in response to arachidonic acid and ristocetin. A severely decreased content of platelet proteins such as fibrinogen, vWF:Ag, BTG and PF4. was further demonstrated. A bone marrow biopsy performed on March 1986 gave no evidence of myelofibrosis (occasionally recorded in GPS) but the patient developed for these last 6 years a severe IPF requiring a permanent oxygen-therapy. Although the association GPS-IPF might be only considered as a fortuitous one, we hypothesize that these two events might be related to each other, possibly through the presence of megakaryocytes in pulmonary capillaries, in the same way as bone marrow fibrosis has been suggested as a possible consequence of the lack of a-granules in GPS (DR0UET et al. - Nouv. Rev. Fr. Hematol., 1981, 23 : 95).