Academic literature on the topic 'Genotypic'

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Journal articles on the topic "Genotypic"

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Sawada, Leila, Andréia Cristina Costa Pinheiro, Daiane Locks, Adriana do Socorro Coelho Pimenta, Priscila Rocha Rezende, Deborah Maia Crespo, José Ângelo Barletta Crescente, José Alexandre Rodrigues de Lemos, and Aldemir Branco de Oliveira Filho. "Distribution of hepatitis C virus genotypes among different exposure categories in the State of Pará, Brazilian Amazon." Revista da Sociedade Brasileira de Medicina Tropical 44, no. 1 (February 2011): 8–12. http://dx.doi.org/10.1590/s0037-86822011000100003.

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INTRODUCTION: Epidemiological studies concerning HCV genotypic distribution in the Brazilian Amazon are scarce. Thus, this study determined the patterns of distribution of HCV genotypes among different exposure categories in the State of Pará, Brazilian Amazon. METHODS: A cross-sectional study was conducted on 312 HCV-infected individuals belonging to different categories of exposure, who were attended at the HEMOPA, CENPREN and a private hemodialysis clinic in Belém. They were tested for HCV antibodies using an immunoenzymatic test, RNA-HCV, using real-time PCR and HCV genotyping through phylogenetic analysis of the 5' UTR. The population groups were epidemiologically characterized according to data collected in a brief interview or medical consultation. RESULTS: Genotype 1 predominated in all the different categories of HCV exposure. HCV genotypic distribution among blood donors comprised genotypes 1 (94%) and 3 (6%). All patients with chronic hematologic diseases had HCV genotype 1. The genotypic distribution in illicit-drug users comprised genotypes 1 (59.6%) and 3 (40.4%). In patients under hemodialysis, genotypes 1 (90.1%), 2 (3.3%), and 3 (6.6%) were detected. Finally, the frequency of genotypes 1 and 3 was significantly different between the groups: BD and DU, PUH and DU, PUH and PCHD and PCHD and DU. CONCLUSIONS: The genotypic frequency and distribution of HCV in different categories of exposure in the State of Pará showed a predominance of genotype 1, regardless of the possible risk of infection.
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Filho, João de Andrade Dutra, Tercilio Calsa Júnior, Djalma Euzébio Simões Neto, Lauter Silva Souto, Anielson dos Santos Souza, Rômulo Gil de Luna, Frank Gomes-Silva, et al. "Genetic divergence for adaptability and stability in sugarcane: Proposal for a more accurate evaluation." PLOS ONE 16, no. 7 (July 15, 2021): e0254413. http://dx.doi.org/10.1371/journal.pone.0254413.

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The best agro-industrial performance presented by a crop genotype in one environment may not be reproduced in another owing to complex edaphoclimatic variations. Therefore, breeding programs are constantly attempting to obtain, through artificial hybridization, novel genotypes with high adaptability and stability potential. The objective of this study was to analyze genetic divergence in sugarcane based on the genotypic values of adaptability and stability. A total of 11 sugarcane genotypes were analyzed for eight agro-industrial traits. The genotypic values of the traits were determined using mixed model methodology, and the genetic divergence based on phenotypic and genotypic values was measured using the Mahalanobis distance. The distance matrices were correlated using the Mantel test, and the genotypes were grouped using the Tocher method. Genetic divergence is more accurate when based on genotypic values free of genotype–environment interactions and will differ from genetic divergence based on phenotypic data, changing the genotype allocations in the groups. The above methodology can be applied to assess genetic divergence to obtain novel sugarcane genotypes with higher productivity that are adapted to intensive agricultural systems using diverse technologies. This methodology can also be tested in other crops to increase accuracy in selecting the parents to be crossed.
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Utsumi, Shunsuke, Yoshino Ando, Timothy P. Craig, and Takayuki Ohgushi. "Plant genotypic diversity increases population size of a herbivorous insect." Proceedings of the Royal Society B: Biological Sciences 278, no. 1721 (March 4, 2011): 3108–15. http://dx.doi.org/10.1098/rspb.2011.0239.

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It is critical to incorporate the process of population dynamics into community genetics studies to identify the mechanisms of the linkage between host plant genetics and associated communities. We studied the effects of plant genotypic diversity of tall goldenrod Solidago altissima on the population dynamics of the aphid Uroleucon nigrotuberculatum . We found genotypic variation in plant resistance to the aphid in our experiments. To determine the impact of plant genotypic diversity on aphid population dynamics, we compared aphid densities under conditions of three treatments: single-genotype plots, mixed-genotype plots and mixed-genotype-with-cages plots. In the latter treatment plants were individually caged to prevent natural enemy attack and aphid movement among plants. The synergistic effects of genotypes on population size were demonstrated by the greater aphid population size in the mixed-genotype treatment than expected from additive effects alone. Two non-exclusive hypotheses are proposed to explain this pattern. First, there is a source–sink relationship among plant genotypes: aphids move from plant genotypes where their reproduction is high to genotypes where their reproduction is low. Second, natural enemy mortality is reduced in mixed plots in a matrix of diverse plant genotypes.
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Rezene, Yayis. "GGE-Biplot Analysis of Multi-Environment Yield Trials of Common Bean (Phaseolus vulgaris L.) in the southern Ethiopia." Journal of Plant Studies 8, no. 1 (February 12, 2019): 35. http://dx.doi.org/10.5539/jps.v8n1p35.

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The present study was conducted on thirty-six common beans (Phaseolus vulgaris L.) Genotypes across six contrasting environments defined for its different soil fertility status and located at the southern Ethiopia. The genotypes were arranged in 6 x 6 triple lattice design and executed for two successive main cropping seasons with the objectives to evaluate yield performance of common bean genotypes and identification of mega environments. GGE (i.e., G = genotype and GE = genotype by environment, interaction) bi-plot methodology was used for graphical presentation of yield data after subjecting the genotypic means of each environment to GGE Bi-plot software. The first two principal components (AXIS 1 and AXIS2) were used to display a two-dimensional GGE bi-plot. Thus, genotypic AXIS1 scores >0 classified the high yielding genotypes while AXIS2 scores <0 identified low yielding genotypes. Unlike genotypic AXIS1, genotypic AXIS2, scores near zero showed stable genotypes whereas large AXIS2 scores classified the unstable ones. The environmental AXIS1 were related to crossover nature of GEI while AXIS2 scores were associated with non-cross over GEI. The six test environments in the southern region were divided in to two distinct mega environments (Mega-1 and 2). Mega-1 constituted GOHF13, ARMF12 and ARLF13 while genotype 14 (SCR10) being the best winner, on the other hand, Mega-2 contained GOHF12 and while common bean genotype 20(SCR17) being the best winner. The results of this study indicated that breeding for specific adaptation should be taken as a breeding strategy in southern region to exploit positive GEI to increase production and productivity of common bean.
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Damé-Teixeira, Nailê, Rodrigo Alex Arthur, Clarissa Cavalcanti Fatturi Parolo, and Marisa Maltz. "Genotypic Diversity and Virulence Traits ofStreptococcus mutansIsolated from Carious Dentin after Partial Caries Removal and Sealing." Scientific World Journal 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/165201.

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The aim of this study was to compare the genotypic diversity and virulence traits ofStreptococcus mutansisolated from carious dentin before and after partial dentin caries removal (PDR) and sealing. Carious dentin samples were obtained three months before and after the PDR and cavity sealing. Up to seven isolates of each morphological type ofS. mutanswere selected and strain identity was confirmed using gtfB primer. Genotyping was performed by arbitrary primer-PCR (AP-PCR). Acidogenesis and acidurance of the genotypes were evaluated as virulence traits. A pairedt-test and a Wilcoxon test were used to compare the virulence of genotypes. A total of 48 representativeS. mutansisolates were genotyped (31 before and 17 after the sealing). At least one of the genotypes found before the sealing was also found on dentin after the sealing. The number of genotypes found before the sealing ranged from 2 to 3 and after the sealing from 1 to 2 genotypes. No difference was observed in the acidogenesis and acidurance between genotypes isolated before and after the sealing. In conclusion, genotypic diversity ofS. mutansdecreased after the PDR and sealing, but the virulence traits ofS. mutansremained unchangeable.
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Stenger, Drake C., and Carrie L. McMahon. "Genotypic Diversity of Beet Curly Top Virus Populations in the Western United States." Phytopathology® 87, no. 7 (July 1997): 737–44. http://dx.doi.org/10.1094/phyto.1997.87.7.737.

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The genotypic diversity of beet curly top virus (BCTV) present in the western United States has been examined by the analysis of 58 field isolates and eight laboratory or nursery isolates of the virus. Full-length clones for each isolate have been characterized for genotype by restriction endonuclease mapping. The results indicate that most of the genotypes examined may be classified as variants of the CFH, Worland, or Cal/Logan strains of BCTV. Two genotypes were recovered that appear to share certain genotypic markers of both Worland and CFH strains. Genotypic variants of the CFH and Worland strains and the two genotypes sharing markers of both strains were recovered from field isolates collected during 1994 and 1995. In contrast, the Cal/Logan strain was recovered only from isolates maintained in laboratories or nurseries. Comparisons of restriction endonuclease maps of cloned BCTV genomes revealed considerable variability both within and between strains. Although a total of 43 distinct genotypes of BCTV were identified, only 36 (84%) were recovered from field isolates. Of 37 field isolates for which more than a single clone was recovered, 16 (43%) contained more than a single genotype of one strain, whereas 4 (11%) harbored mixed infections of the CFH and Worland strains. A phylogenetic analysis using 43 characters derived from restriction endonuclease mapping data supported the grouping of 41 genotypes into three taxa consistent with the three currently recognized strains of BCTV. The relationships of the two genotypes sharing genotypic markers of both the Worland and CFH strains to other BCTV genotypes was unresolved in the phylogenetic analysis. Based on the mild symptom phenotype of the isolates from which these two genotypes were recovered and the presence of Worland genotypic markers in portions of the genome containing both cis- and trans-acting elements determining replication specificity, these two genotypes were tentatively considered as variants of the Worland strain.
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Sabaghnia, Naser, Mohtasham Mohammadi, and Rahmatollah Karimizadeh. "Clustering Durum Wheat Genotypes in Multi-Environmental Trials of Rain-Fed Conditions." Plant Breeding and Seed Science 66, no. 1 (August 8, 2014): 119–37. http://dx.doi.org/10.2478/v10129-011-0063-5.

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AbstractFor durum wheat genotypes evaluation in multi-environmental trials (MET), measured seed yield is the combined result of effects of genotype (G), environment (E) and genotype by environment GE interaction. The GE interaction structure can be identified if the data are stratified into homogeneous subsets through cluster analysis. A combined analysis to assess GE interactions of 20 durum wheat genotypes across 14 environments was undertaken. The combined analysis of variance for E, G and GE interaction was significant, suggesting differential responses of the genotypes in various environments. Four cluster methods, which differ in the dissimilarity indices depending on the regression model or ANOVA model, were used. According to dendograms of regression methods there were 10 different genotypic groups based on G (intercept) and GE (line slope) sources and 3 different genotypic groups based on GE (line slope) sources. Also, the dendograms of ANOVA methods indicated 11 different genotypic groups based on G and GE sources and 13 different genotypic groups based on GE sources. The above mentioned genotypic groups were determined via F-test as an empirical stopping criterion for clustering. Due to the high values of regression’s determination coefficient which ranged from 92.6 to 99.4, using of the linear regression-based clustering was more practical. The genotypes clustering based on similarity of linear regression parameters or ANOVA model indicated that there were considerable variations among durum wheat genotypes and there are different with each other in response to environmental changes. Such an outcome could be regularly applied in the future to clattering durum wheat genotypes and other crops based on regression or ANOVA models in the Middle East and other areas of the world.
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Ul-Rahman, Aziz. "Genotypic and Sub-genotypic Diversity of Avian Paramyxoviruses 2, 4 and 6." Pakistan Veterinary Journal 41, no. 01 (March 1, 2021): 156–59. http://dx.doi.org/10.29261/pakvetj/2020.088.

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Avian paramyxoviruses (APMVs) are contagious viruses infecting multiple avian species around the globe. Subsequent to evolution, the emergence of new strains is considered to cause outbreaks worldwide. Using standard classification criteria, genotypic and sub-genotypic distribution of strains within APMV-1 is much elucidated across the globe. Nevertheless, despite a growing number of genome sequencing data for APMVs excluding those that are prototypes, there is an absolute paucity of an updated unified phylogenetic classification scheme for APMV-2, 4, and 6. Utilizing a well-recognized genetic marker (complete fusion gene), genotyping and sub-genotyping of under-studied of APMVs strains was proposed by the implementation of different reliable tools and criteria. The analysis categorized the strains of each APMV-2 and 6 into two distinct genotypes (I and II), whereas APMV-4 strains categorized into three genotypes (I, II, and III). Additionally, a total of four sub-genotypes within APMV-6 (I.1, I.2, II.1, and II.2) and five sub-genotypes (I.1, I.2, II.1, II.2, and II.3) within APMV-4 were also proposed in the current study. Though it may require a revision and update in the future with the abundance of genome sequences and subsequent analysis from wide geography, herein the outcomes are relevant to better elucidate the classification and molecular epidemiology of study-included APMVs to depict an evolution and epidemiological link among outbreaks caused by field circulatory strains.
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Arellano-Galindo, José, Dina Villanueva-García, José Luis Cruz-Ramirez, Juan Pablo Yalaupari-Mejìa, Gabriel Uribe-Gutiérrez, Norma Velazquez-Guadarrama, Margarita Nava-Frias, Onofre Munoz-Hernández, and Juan Manuel Mejía-Arangure. "Detection and gB genotyping of CMV in Mexican preterm infants in the context of maternal seropositivity." Journal of Infection in Developing Countries 8, no. 06 (June 11, 2014): 758–67. http://dx.doi.org/10.3855/jidc.3501.

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Introduction: Congenital (CI) and perinatal cytomegalovirus (CMV) infections (PI) can be linked to maternal CMV seropositivity, with fatal consequences in preterm newborns. GB genotyping has been used to analyze genotypic similarity in mothers and infants. The frequency of CMV infection in the context of maternal seropositivity and the viral gB genotypes as well as the genotypic similarity in mothers and preterm infants were investigated. Methodology: Saliva samples and dry blood spots (DBS) were taken weekly from preterm newborns from birth until the first month of life, and breast milk samples were taken from their mothers weekly during the first month of lactation. CMV IgG seroprevalence of the mothers and CI or PI in the infants were established. The gB status and genotypic similarities were established retrospectively in DBS and in the breast milk samples. Results: In total, 387 neonates and 375 mothers were enrolled. The maternal CMV-positive IgG serology was 97.3% (365/375). Neonatal CMV was found in 5.1% (20/387) of newborns, and one infant presented with CMV-compatible symptoms. CI was 2.5% and PI in the first month after birth was 11.8%. GB2 was the most prevalent genotype and was also the genotype preferentially transmitted to newborns by mothers with mixed infections. Conclusions: CMV PI and CI in preterm infants from highly seropositive mothers was high, but the rate of symptomatic infection was low. The prevalent genotype was gB2, and this genotype was preferentially transmitted to newborns by mothers with mixed infections.
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Dirac, M. Ashworth, Kris M. Weigel, Mitchell A. Yakrus, Annie L. Becker, Hui-Ling Chen, Gina Fridley, Arthur Sikora, et al. "Shared Mycobacterium avium Genotypes Observed among Unlinked Clinical and Environmental Isolates." Applied and Environmental Microbiology 79, no. 18 (July 12, 2013): 5601–7. http://dx.doi.org/10.1128/aem.01443-13.

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ABSTRACTOur understanding of the sources ofMycobacterium aviuminfection is partially based on genotypic matching of pathogen isolates from cases and environmental sources. These approaches assume that genotypic identity is rare in isolates from unlinked cases or sources. To test this assumption, a high-resolution PCR-based genotyping approach, large-sequence polymorphism (LSP)-mycobacterial interspersed repetitive unit–variable-number tandem repeat (MIRU-VNTR), was selected and used to analyze clinical and environmental isolates ofM. aviumfrom geographically diverse sources. Among 127 clinical isolates from seven locations in North America, South America, and Europe, 42 genotypes were observed. Among 12 of these genotypes, matches were seen in isolates from apparently unlinked patients in two or more geographic locations. Six of the 12 were also observed in environmental isolates. A subset of these isolates was further analyzed by alternative strain genotyping methods, pulsed-field gel electrophoresis and MIRU-VNTR, which confirmed the existence of geographically dispersed strain genotypes. These results suggest that caution should be exercised in interpreting high-resolution genotypic matches as evidence for an acquisition event.
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Dissertations / Theses on the topic "Genotypic"

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Alghhamdi, Saad Saeed. "Genotypic analysis of 'mycobacterium tuberculosis'." Thesis, London School of Hygiene and Tropical Medicine (University of London), 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.536847.

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Edwards, Andrew M. "Genotypic and phenotypic diversity in Treponema." Thesis, University of Bristol, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.392906.

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Marcar, Nico Emile. "Genotypic variation for manganese efficiency in cereals /." Title page, abstract and contents only, 1986. http://web4.library.adelaide.edu.au/theses/09PH/09phm313.pdf.

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Natale, Alessandra Pia. "Genotypic and phenotypic flexibility of microbial communities." Thesis, University of Warwick, 2010. http://wrap.warwick.ac.uk/35530/.

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Paracoccus denitrificans is a facultative anaerobic Gram-negative α- proteobacterium able to shift to denitrification under anaerobic conditions (John & Whatley, 1978; Zumft, 1997). Because of its metabolic versatility, P. denitrificans has been used in this study as a model organism to investigate the role of environmental heterogeneity in maintaining metabolic flexibility in bacterial communities. The hypotheses underlying this study are: - Metabolic flexibility is maintained in situ by environmental heterogeneity and, specifically: - Under constant environmental conditions the metabolic flexibility of the generalist P. denitrificans will be lost by accumulation of mutations in unused genes. Chemostat cultures under constant aerobic conditions revealed how after ~150 generations genetic loci not in use under aerobic conditions (in particular nirS and nosZ) are subjected to a lower selective pressure that leads to a higher genetic polymorphism in the population. The phenotypic analysis of the population resulting from the same chemostat culture showed a lower specific growth rate and a higher yield compared to the ancestor population, suggesting a deactivation of concomitant denitrification and aerobic respiration (Robertson et al., 1988). Furthermore, the resulting population shows a down-regulation of expression of all three denitrification genes tested and a lower production of nitrous oxide (N2O). When cultured in batch cultures for a long period of time under aerobic conditions, P. denitrificans shows a similar adaptative response. Four parallel populations, originated from a common ancestor and propagated aerobically for more than 500 generations undertook some important communal genotypic and phenotypic changes that suggested that P. denitrificans repetitively adapts to constant environmental conditions by losing its characteristic metabolic flexibility. By following the first steps of loss of metabolic flexibility as an adaptative response to novel environmental conditions in a generalist model as P. denitrificans we could empirically witness the important role of the environment on bacterial evolution and speciation.
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Hoek, Kim Gilberte Pauline. "Investigation into genotypic diagnostics for mycobacterium tuberculosis." Thesis, Stellenbosch : Stellenbosch University, 2010. http://hdl.handle.net/10019.1/5479.

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Thesis (PhD )--University of Stellenbosch, 2010.
ENGLISH ABSTRACT: Diagnostic delay is regarded as a major contributor to the continuous rise in tuberculosis (TB) cases and the emergence and transmission of multidrug-resistant tuberculosis (MDR-TB) and extensively drug resistant tuberculosis (XDR-TB). It is therefore essential that more rapid diagnostic methods are developed. Molecular-based assays have the potential for the rapid species-specific diagnosis of TB and associated drug-resistances directly from clinical specimens. We investigated whether high resolution melting analysis (HRM) could enable the rapid diagnosis of TB and associated drug resistance, since the HRM apparatus and reagents are relatively inexpensive and the methodology can easily be implemented in high incidence, low income regions. Application of this methodology allowed for the rapid identification of mycobacterial lymphadenitis from fine-needle aspiration biopsy (FNAB) samples in 2 studies. This was done by targeting the region of deletion 9 (RD9), present in M. tuberculosis and M. canettii, but absent from all other members of the complex. However, the sensitivity of the method was low (51.9% and 46.3%, respectively) when compared to the reference standard (positive cytology and/or positive culture). Despite this limitation our method was able to provide a rapid diagnosis in more than half of the infected patients with a relatively high specificity (94.0% and 83.3%, respectively). We therefore proposed a diagnostic algorithm allowing the early treatment of patients with both HRM and cytology results indicative of mycobacterial disease. We developed the Fluorometric Assay for Susceptibility Testing of Rifampicin (FAST-Rif) which allowed the rapid diagnosis of MDR-TB by detecting rifampicin (RIF) resistance mutations in the rpoB gene with a sensitivity and specificity of 98% and 100%, respectively. The FAST-Rif method was easily adapted to detect ethambutol (EMB) resistance due to mutations in the embB gene with a sensitivity and specificity of 94.4% and 98.4% respectively, as compared to DNA sequencing. The FAST-EMB method was a significant improvement over the inaccurate culture based method. We identified a strong association between EMB resistance (and pyrazinamide resistance) and MDR-TB and subsequently advised modifications to the current (2008) South African National TB Control Programme draft policy guidelines. Due to the potential for amplicon release, we adapted the FAST-Rif and FAST-EMB methods to a closed-tube one-step method using the detection of inhA promoter mutations conferring isoniazid (INH) resistance as a model. The method (FASTest-inhA) was able to identify inhA promoter mutations with a sensitivity and specificity of 100% and 83.3%. These mutations are of particular interest as they confer low level INH resistance and cross-resistance to ethionamide (Eto). Since inhA promoter mutations are strongly associated with XDR-TB in the Western and Eastern Cape Provinces of South Africa, data generated by the recently implemented GenoType® MDRTBPlus assay may allow individualised treatment regimens to be designed for a patient depending on their INH mutation profile. Our proposed treatment algorithm may be particularly useful in XDR-TB cases, for which only few active drugs remain available. Since current diagnostic methods all carry advantages and disadvantages, a combination of phenotypic and genotypic-based methodologies may be the best scenario while awaiting superior methods.
AFRIKAANSE OPSOMMING: Die onvermoë om tuberkulose (TB), multi-weerstandige tuberkulose (MDR-TB) en uiters weerstandige tuberkulose (XDR-TB) vinnig te diagnoseer, is ‘n belangrike oorsaak vir die volgehoue toename en verspreiding daarvan. Dit is noodsaaklik dat diagnostiese toetse wat vinniger resultate oplewer, ontwikkel word. Molukulêre toetsing het die potensiaal om vinnig spesie-spesifieke diagnoses van TB en die weerstandigheid teen TB-medikasie te lewer. Hierdie studie wil vasstel of hoë-resolusie smeltingsanalise (HRS) ‘n vinnige diagnose van TB en die weerstandigheid teen TB-medikasie kan oplewer aangesien die relatiewe lae koste van reagense en apparaat, asook die minimale infrastruktuur en vaardighede wat vir dié toets benodig word, dit uiters geskik maak vir pasiënte in gebiede met ‘n hoë TB-insidensie en lae inkomste. Die toepassing van die HRS-metode op fynnaald-aspiraatbiopsies in twee afsonderlike studies, het gelei tot die vinnige identifisering van mikrobakteriële-limfadenitis. Dit is bemiddel deur die gebied van delesie 9 (RD9) teenwoordig in Mycobacterium tuberculosis en M. canettii, maar afwesig in al die ander lede van die kompleks, te teiken. Die sensitiwiteit van die metode was (51.9% en 46.3%, vir die twee studies onderskeidelik) in vergelyking met die verwysingstandaard (positiewe sitologie en/of positiewe kultuur). Ten spyte van dié beperking was ‘n vinnige diagnose in meer as die helfte van geïnfekteerde pasiënte met ‘n redelike hoë spesifisiteit (94.0% en 83.3%, onderskeidelik) moontlik. ‘n Diagnostiese algoritme wat gebaseer is op die resultate van die HRS en sitologie-toetse, is voorgestel om pasiënte vroeër te behandel. ‘n Fluorometriese toets (FAST-Rif) is ontwikkel vir die vinnige diagnose van MDR-TB deur mutasies in die rpoB-geen op te spoor met ‘n hoë sensitiwiteit en spesifisiteit (98% en 100%, onderskeidelik). Hierdie mutasies is verantwoordelik vir weerstandigheid teen die antibiotikum rifampicin (FAST-Rif) en word beskou as ‘n vinnige diagnose vir MDR-TB. Die FAST-Rif metode kon maklik aangepas word om mutasies in die embB-gene, verantwoordelik vir weerstandigheid teen die antibiotikum ethambutol (EMB), op te spoor. Die FAST-EMB-metode het ‘n sensitiwiteit en spesifisiteit van 94.4% en 98.4% onderskeidelik getoon in vergelyking met DNS volgordebepaling. Die FAST-EMB-metode was ‘n betekenisvolle verbetering op die onakkurate kultuurgebaseerde metodes. ‘n Sterk korrelasie tussen EMB-weerstandigheid (en weerstandigheid teen pyrazinamide) en MDR-TB is geïdentifiseer. Vervolgens is veranderinge aan die Suid-Afrikaanse Nasionale TB-beheerprogram se Konsepbeleidsgids (2008) voorgestel. Om die potensiële vrylating van amplikone te verhoed, is die FAST-Rif en FAST-EMB aangepas tot ‘n enkelstap geslote buissisteem deur gebruik te maak van die opsporing van inhA promotormutasies wat weerstandigheid teen isoniazid (INH) veroorsaak. Die metode het ‘n sensitiwiteit en spesifisiteit van 100% en 83.3% onderskeidelik, getoon. Hierdie mutasies veroorsaak laevlak weerstandigheid teen INH, maar ook kruisweerstandigheid teen ethionamide (Eto). Aangesien daar ‘n sterk verbintenis tussen inhA-promotormutasies en XDR-TB in die Oos en Wes-Kaapprovinsies van Suid-Afrika is, kan data van die GenoType® MDRTBPlus-toets moontlik gebruik word om ‘n meer geïndividualiseerde behandeling te ontwerp afhangende van die pasiënt se INH-mutasieprofiel. Ons behandelingsalgoritme is veral geskik vir XDR-TB pasiënte vir wie daar weinig aktiewe antibiotika beskikbaar is. Huidige diagnostiese metodes het almal voor- en nadele, dus bied ‘n kombinasie van fenotipiese en genotipiese metodes moontlik die beste oplossing totdat beter metodes ontwikkel word.
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Rayment, Sarah Jayne. "Phenotypic and genotypic analysis of intestinal spirochaetes." Thesis, Aston University, 1998. http://publications.aston.ac.uk/10969/.

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Pulsed field gel electrophoresis of 82 intestinal spirochaete isolates showed specific differentiation of Serpulina pilosicoli and Serpulina hyodysenteriae although considerable heterogeneity was observed, especially amongst S. pilosicoli isolates. In several cases genotypically similar isolates originated from different animals suggesting that cross-species transmission may have occurred. The Caco-2 and Caco-21HT29 cell models have been proposed as potentially realistic models of intestinal infection. Quantitation of adhesion to the cells showed isolate 3 82/91 (from a bacteraemia) to adhere at significantly greater numbers than any other isolate tested. This isolate produced a PFGE profile which differed from other S. pilosicoli isolates and so would be of interest for further study. Comparison of bacteraemic and other S. pilosicoli isolates suggested that bacteraemic isolates were not more specifically adapted for adhesion to, or invasion of the epithelial cell layer than other S. pilosicoli isolates. Genotypically similar isolates from differing animal origins adhered to the Caco-2 model at similar levels. Generation of a random genomic library of S. pilosicoli and screening with species specific monoclonal antibody has enabled the identification of a gene sequence encoding a protein which showed significant homology with an ancestral form of the enzyme pyruvate oxidoreductase. Immunoscreening with polyclonal serum identified the sequences of two gene clusters and a probable arylsulphatase. One gene cluster represented a ribosomal gene cluster which has a similar molecular arrangement to Borrelia burgdorjeri, Treponema pallidum and Thermatoga maritima. The other gene cluster contained an ABC transporter protein, sorbitol dehydrogenase and phosphomannose isomerase. An ELISA type assay was used to demonstrate that isolates of S. pilosicoli could adhere to components of the extracellular matrix such as collagen (type 1), fibronectin, laminin, and porcine gastric mucin.
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Green, Lauren M. "Phenotypic and genotypic characterisation of Clostrum Difficile." Thesis, Aston University, 2010. http://publications.aston.ac.uk/9584/.

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Clostridium difficile is at present one of the most common nosocomial infections in the developed world. Hypervirulent strains (PCR ribotype 027) of C. difficile which produce enhanced levels of toxins have also been associated with other characteristics such as a greater rate of sporulation and resistance to fluoroquinolones. Infection due to C. difficile PCR ribotype 027 has also been associated with greater rates of morbidity and mortality. The aim of this thesis was to investigate both the phenotypic and genotypic characteristics of two populations of toxigenic clinical isolates of C. difficile which were recovered from two separate hospital trusts within the UK. Phenotypic characterisation of the isolates was undertaken using analytical profile indexes (APIs), minimum inhibitory concentrations(MICs) and S-layer protein typing. In addition to this, isolates were also investigated for the production of a range of extracellular enzymes as potential virulence factors. Genotypic characterisation was performed using a random amplification of polymorphic DNA(RAPD) PCR protocol which was fully optimised in this study, and the gold standard method, PCR ribotyping. The discriminatory power of both methods was compared and the similarity between the different isolates also analysed. Associations between the phenotypic and genotypic characteristics and the recovery location of the isolate were then investigated. Extracellular enzyme production and API testing revealed little variation between the isolates; with S-layer typing demonstrating low discrimination. Minimum inhibitory concentrations did not identify any resistance towards either vancomycin or metronidazole; there were however significant differences in the distribution of antibiogram profiles of isolates recovered from the two different trusts. The RAPD PCR protocol was successfully optimised and alongside PCR ribotyping, effectively typed all of the clinical isolates and also identified differences in the number of types defined between the two locations. Both PCR ribotyping and RAPD demonstrated similar discriminatory power; however, the two genotyping methods did not generate amplicons that mapped directly onto each other and therefore clearly characterised isolates based on different genomic markers. The RAPD protocol also identified different subtypes within PCR ribotypes, therefore demonstrating that all isolates defined as a particular PCR ribotype were not the same strain. No associations could be demonstrated between the phenotypic and genotypic characteristics observed; however, the location from which an isolate was recovered did appear to influence antibiotic resistance and genotypic characteristics. The phenotypic and genotypic characteristics observed amongst the C. difficile isolates in this study, may provide a basis for the identification of further targets which may be potentially incorporated into future methods for the characterisation of C. difficile isolates.
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Gorman, Gráinne S. "Clinical and genotypic aspects of mitochondrial disease." Thesis, University of Newcastle upon Tyne, 2015. http://hdl.handle.net/10443/3034.

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Mitochondrial myopathies are a clinically multifarious group of genetic disorders that affect the central nervous system and skeletal muscles and other organs heavily dependent on aerobic metabolism. They are typically characterised by multi-system involvement and have extensive phenotypic and disease burden variability. These diseases are often relentlessly progressive with high morbidity and mortality. The biochemical and molecular basis of many of the common mitochondrial myopathies has been elucidated over the last decade, yet the association between mitochondrial gene mutations and clinical symptoms, requires further elucidation. I propose to clearly define the clinico-pathological and molecular features of adults with mitochondrial disease and evaluate if there is a clear correlation between clinical phenotype and the underlying genetic defect. Identifying clear clinical features should help guide genetic diagnosis and enable tailored counselling regarding potential disease progression. Unfortunately, to date, there are few effective treatments and no known cure for patients with mitochondrial myopathies. Exercise has been shown to hold significant positive effects upon skeletal muscle function and perceived health- related quality of life in patients with mitochondrial myopathies. The molecular basis of many of the common mitochondrial disorders has been elucidated over the last decade and although there is a vast spectrum of phenotypic expression throughout different genotypes, common symptoms are reported. Perceived fatigue is often a prominent symptom in patients with mitochondrial disease but to date, its prevalence, severity and aetiology is poorly understood. I wish to determine the prevalence and nature of perceived fatigue in a large, genetically heterogeneous group of patients with mitochondrial disease and systematically assess potential covariates of fatigue compared to healthy controls and patients with Myalgic Encephalopathy /Chronic Fatigue Syndrome. Health-related quality of life is important for understanding the impact and progression of chronic disease and is increasingly recognised as a fundamental patient-based outcome measure in both clinical intervention and research. Generic outcome measures have been extensively validated to assess health-related quality of life across populations and different disease states. However, due to their inclusive construct, it is acknowledged that not all relevant aspects of a specific illness may be captured. Hence there is a need to develop a disease-specific health-related quality of life measures that centre on symptoms characteristic of a specific disease or condition and their impact. SF-36 and its abbreviated version SF-12 are currently the only tools used routinely for measuring patient-reported outcomes in our patients with mitochondrial myopathies. I wish to explore the conceptualisation, development and preliminary psychometric evaluation (validity and reliability) of a mitochondrial disease -specific health-related quality of life measure, which may be used both in research and clinical settings. Indeed, in a condition where the natural history of the disease is poorly understood and therapeutic options are limited, long-term preservation of health-related quality of life in patients with mitochondrial disease poses a real challenge.
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Gilchrist, Tamara Louise. "Genotypic and phenotypic characterisation of Streptococcus uberis." Thesis, University of Glasgow, 2011. http://theses.gla.ac.uk/2938/.

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Streptococcus uberis is an important bovine mastitis pathogen, which places a significant financial burden upon the dairy industry. Determining the genetic diversity of a collection of field isolates and the mechanisms by which S. uberis colonises the host were the general aims of this project, in particular the determination of the basis for bacterial persistence despite antibacterial therapy. Multi-locus sequence typing identified high levels of recombination within the population, but also a single dominant clonal complex which comprised nearly all sequence types which were isolated from more than one animal. The dominant clonal complex also comprised isolates, derived, however, from both persistent and non-persistent infections, but RAPD typing demonstrated that these isolates can differ in genetic composition elsewhere in the genome. Whole genome sequencing of additional S. uberis isolates confirmed that despite significant homology between much of these genomes, novel genetic material was commonly obtained by phage insertion and horizontal gene transfer. Isolates with identical housekeeping sequences are thus highly likely to differ in their virulence gene repertoires. In this study, the potential for differentiating S. uberis isolates based instead upon protein profiles derived from mass spectrometry of disrupted whole cells was therefore also explored. Differentiation between small numbers of isolates was achieved after optimisation of this protocol, however, discriminatory ability and reproducibility were somewhat compromised when the technique was scaled up to analyse 50 Italian isolates. During the period of study, profile differences between persistent and non-persistent isolates could not be explored. Basic methods were thus also utilised in an attempt to identify factors which promoted bacterial survival in vitro; and a defined medium, representative of the udder environment, was optimised for this purpose. The use of this medium permitted the demonstration that S. uberis was reliant upon magnesium and manganese for proliferation and that, interestingly, the absence of iron did not inhibit bacterial growth. It was also shown that S. uberis had the ability to directly utilise casein, identifying a potential alternative pathway for the acquisition of essential nutrients from nutritionally-limited environments. It was also observed that to a limited extent S. uberis seemed to produce a siderophore. Although this remains to be confirmed, it may correlate with the observation that iron, although not essential for proliferation, improved the growth rate of the bacterium. It was also notable that most novel genes, identified from S. uberis genome sequences, exhibited functions for nutrient metabolism, demonstrating that flexibility in nutrient acquisition is central to the ability of the bacteria to adapt, permitting survival in vastly different environments. The use of the defined medium also demonstrated that S. uberis was able to form biofilms; this ability being variable depending on the growth conditions used and the isolate studied. Most significantly, under conditions representative of the mammary gland, there was an apparent trend for high levels of biofilm formation to correlate with isolates from persistent infections. Biofilm formation by Staphylococcus aureus is considered to be pivotal to the development of chronic mastitis, thus, biofilms may similarly play a role in S. uberis persistence. In an attempt to identify the molecular basis for S. uberis biofilm formation, genes with homology to those of the intercellular adhesion (ica) operon, well described for their involvement in Staphylococcus epidermidis and S. aureus biofilm formation, were identified in the genome sequence of S. uberis 0140J. A targeted mutagenesis protocol was optimised to ‘knock out’ these genes and observe the subsequent effects of these mutations on biofilm formation. During the course of this study, two of these potential biofilm genes (hasA and SUB 0809) were deleted from the S. uberis 0140J chromosome. Surprisingly, deletion of these genes did not retard subsequent biofilm formation, but instead biofilm formation was dramatically improved in the mutant strains. Characterisation of mastitis-causing S. uberis strains and a detailed understanding of the pathogenicity of the organism are required to further the development of a successful vaccine. The research presented in this thesis has increased the knowledge of these important research objectives and optimised techniques which will allow further advancement of knowledge in this field.
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Bhatnagar, Sandeep. "Phenotypic and genotypic characterization of high lysine maize." Texas A&M University, 2004. http://hdl.handle.net/1969.1/3154.

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Quality Protein Maize (QPM) with the mutant gene opaque-2 (o2), has higher lysine and tryptophan content and hard endosperm which is less susceptible to mechanical and biological damage. Three experiments were conducted to characterize the phenotypic and genotypic characteristics of high lysine maize. In the first experiment two separate diallels including 7 white and 9 yellow QPM inbreds were evaluated in five southern USA environments to estimate the general (GCA) and specific combining abilities (SCA) for grain yield and to identify potential heterotic relationships among them. QPM hybrids yielded less than commercial checks. GCA effects across environments were non-significant for grain yield but highly significant for secondary traits. Best yielding hybrids resulted from crosses among inbreds from different programs (CIMMYT, Mexico; University of Natal, South Africa and TAMU, USA). In the second experiment testcrosses between QPM inbreds and Tx804, were evaluated for agronomic performance, aflatoxin resistance and quality. QPM inbreds in testcrosses have similar flowering dates, plant height, ear height and test weights but lower grain yield than normal checks. Population 69 inbreds and their testcrosses were least susceptible to aflatoxin. Aflatoxin in testcrosses was positively correlated with endosperm texture (0.67) and kernel integrity (0.60) but negatively correlated with grain yield (-0.30) and silking date (-0.50). Tryptophan content was negatively correlated with endosperm modification. Amino acid levels of inbred lines were significantly correlated with those of hybrids, but with low predictive value. In the third experiment 92 high lysine maize inbreds with different origins [Stiff Stalk, Non Stiff Stalk, Pop 69, temperate (Tx802, Tx804, Tx806, B97, B104) and exotic subtropical lines (CML161, Do940y and Ko326y)] were haplotyped on a cM scale utilizing 43 mapped SSR markers to characterize genetic diversity on chromosome 7, estimate linkage disequilibrium around opaque-2 locus and determine the parental contribution in some inbreds. Dendrograms of genetic similarity showed clusters in agreement with the different origin of inbreds. A total of 200 alleles were detected with an average of 4.7 alleles/locus. Significant linkage disequilibrium was detected around opaque-2 locus. Parental contributions of haplotypes showed segments of chromosome 7 exclusively contributed by one or the other parent.
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Books on the topic "Genotypic"

1

Whyte, Thomas. A study of Yersinia enterocolitica using phenotypic and genotypic methods. [S.l: The Author], 1994.

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Henry, R. J., ed. Plant diversity and evolution: genotypic and phenotypic variation in higher plants. Wallingford: CABI, 2005. http://dx.doi.org/10.1079/9780851999043.0000.

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Kerr, Gifford Read Douglas. Phenotypic and genotypic studies of Haemophilus species from an isolated Antarctic community. Manchester: University of Manchester, 1995.

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Phillips, Lesley. The distribution of phenotypic and genotypic characters within streptomycetes and their relationship to antibioticproduction. [s.l.]: typescript, 1992.

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Turner, Dorothea. The migratory behaviour of lotic macroinvertebrates, and the implications of genotypic and phenotypic variation in studies of their dispersal and response to environmental change. Ottawa: National Library of Canada, 1996.

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White, Stefan J., and Stuart Cantsilieris, eds. Genotyping. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6442-0.

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Batley, Jacqueline, ed. Plant Genotyping. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-1966-6.

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Single-pollen genotyping. Tokyo: Springer, 2011.

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Frommlet, Florian, Małgorzata Bogdan, and David Ramsey. Phenotypes and Genotypes. London: Springer London, 2016. http://dx.doi.org/10.1007/978-1-4471-5310-8.

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Isagi, Yuji, and Yoshihisa Suyama, eds. Single-Pollen Genotyping. Tokyo: Springer Japan, 2011. http://dx.doi.org/10.1007/978-4-431-53901-8.

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Book chapters on the topic "Genotypic"

1

Doolittle, Donald P. "Genotypic Drift." In Advanced Series in Agricultural Sciences, 104–6. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-71734-5_22.

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van den Berg, H. Marijke, and Kathelijn Fischer. "Phenotypic-Genotypic Relationship." In Textbook of Hemophilia, 33–37. Oxford, UK: Wiley-Blackwell, 2010. http://dx.doi.org/10.1002/9781444318555.ch4.

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Huletsky, A., and M. G. Bergeron. "Genotypic Drug Resistance Assays." In Antimicrobial Drug Resistance, 1227–48. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-595-8_39.

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Aguilar, Fernando, and Peter Cerutti. "Genotypic Mutation Assay (RFLP/PCR)." In Technologies for Detection of DNA Damage and Mutations, 431–38. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-0301-3_31.

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Huletsky, A., and Michel G. Bergeron. "Bacterial Genotypic Drug Resistance Assays." In Antimicrobial Drug Resistance, 1465–99. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-47266-9_37.

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Hebert, Paul D. N. "Genotypic characteristics of the Cladocera." In Cladocera, 183–93. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4039-0_21.

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van der Beek, Martha T., and Eric C. J. Claas. "Phenotypic and Genotypic Antiviral Susceptibility Testing." In Clinical Virology Manual, 201–27. Washington, DC, USA: ASM Press, 2016. http://dx.doi.org/10.1128/9781555819156.ch16.

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Laksono, N. D., U. Setiawati, F. Nur, M. Rahmaningsih, Y. Anwar, H. Rusfiandi, B. P. Forster, E. H. Sembiring, A. S. Subbarao, and H. Zahara. "Pre-field genotypic screening and selection." In Nursery practices in oil palm: a manual, 86–91. Wallingford: CABI, 2019. http://dx.doi.org/10.1079/9781789242140.0086.

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Rudenko, I. S., and I. I. Rudenko. "Genotypic variation in apple × quince progenies." In Developments in Plant Breeding, 229–33. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0467-8_47.

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Singleton, Paul. "DNA-Based (‘Genotypic’) Antibiotic Susceptibility Testing." In DNA Methods in Clinical Microbiology, 203–28. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-1286-6_8.

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Conference papers on the topic "Genotypic"

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Malyuzhenets, Nikolay, Nikolay Kozlov, Evgenia Malyuzhenets, and Tatyana Kozlova. "Variability of fodder value of collection samples bentgrass." In Multifunctional adaptive fodder production. ru: Federal Williams Research Center of Forage Production and Agroecology, 2021. http://dx.doi.org/10.33814/mak-2021-25-73-59-66.

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The peculiarities of the manifestation of variability and interrelationships of feed quality indicators in 27 genotypes belonging to three types of bentgrass were studied. A significant level of genotypic variability in the content of crude fat, crude protein, ash, phosphorus and potassium was revealed. Taking into account the low level of variability depending on the conditions of the growing year, it is recommended to pay attention to the crude fat content, crude fiber content and plant height when selecting. It is recommended to determine the genotypic divergence of genotypes when selecting parent pairs for hybridization using cluster analysis methods.
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Al-Husain, Luluah, and Alaaeldin M. Hafez. "Dimensionality reduction approach for genotypic data." In 2015 IEEE Conference on Computational Intelligence in Bioinformatics and Computational Biology (CIBCB). IEEE, 2015. http://dx.doi.org/10.1109/cibcb.2015.7300305.

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Henderson, Charles M. "MORPHOLOGIC VARIABILITY OF SWEETOGNATHIDS: PHENOTYPIC OR GENOTYPIC?" In GSA Annual Meeting in Phoenix, Arizona, USA - 2019. Geological Society of America, 2019. http://dx.doi.org/10.1130/abs/2019am-341190.

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Araujo, Lourdes, Juan Julian Merelo, Antonio Mora, and Carlos Cotta. "Genotypic differences and migration policies in an island model." In the 11th Annual conference. New York, New York, USA: ACM Press, 2009. http://dx.doi.org/10.1145/1569901.1570080.

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Grigoryan, M. A., A. A. Starchikov, O. V. Tkachenko, G. L. Burygin, and N. V. Evseeva. "Varietal features of potato responses to Azospirillum and exogenous indole-3-acetic acid." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.094.

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The effect of A. brasilense Sp245 and indoleacetic acid (IAA) on microplants of 10 potato cultivars in in vitro culture was studied. Significant genotypic differences in the growth-stimulating effect of bacteria and IAA on plants were established.
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Wang, Mingzhao, Xiaoli Wang, Yuping Wang, and Zhen Wei. "An Adaptive Co-evolutionary Algorithm Based on Genotypic Diversity Measure." In 2014 Tenth International Conference on Computational Intelligence and Security (CIS). IEEE, 2014. http://dx.doi.org/10.1109/cis.2014.172.

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Maia, Luciana Furlaneto, Mayara Baptistucci Ogaki, and Márcia Cristina Furlaneto. "Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources." In XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-003.

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Puppo, Gianfranco, Francesco Pistelli, Stefano Fogli, Marzia Del Re, Marco Lazzeretti, Ferruccio Aquilini, Romano Danesi, Laura Carrozzi, and Antonio Palla. "PLASMA/SALIVA AND GENOTYPIC/PHENOTYPIC DIFFERENCES OF NICOTINE METABOLITE RATIO." In ERS International Congress 2018 abstracts. European Respiratory Society, 2018. http://dx.doi.org/10.1183/13993003.congress-2018.oa3810.

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Hilliam, Y. K., A. Perry, A. J. Hall, J. Davison, K. E. Walton, J. Fothergill, A. De Soyza, and C. Winstanley. "Genotypic studies ofpseudomonas aeruginosaisolates from adult non-cystic fibrosis bronchiectasis patients." In Annual Congress 2015. European Respiratory Society, 2015. http://dx.doi.org/10.1183/13993003.congress-2015.pa5039.

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Gebreyes, Wondwossen A., Siddhartha Thakur, and Julie A. Funk. "Molecular Epidemiology of Salmonella enterica and Subtyping Using Phenotypic and Genotypic Approaches." In Second International Symposium on Epidemiology and Control of Salmonella in Pork. Iowa State University, Digital Press, 2003. http://dx.doi.org/10.31274/safepork-180809-447.

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Reports on the topic "Genotypic"

1

Garrity, George, and Charles Parker. The NamesforLife Semantic Index of Phenotypic and Genotypic Data. NamesforLife, LLC, May 2012. http://dx.doi.org/10.1601/report.sc0006191p1.

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Kukurina, Borislava, Milena Georgieva, and George Miloshev. Genotypic Profile Changes in Taraxacum officinale Populations as a Response to Environmental Pollution with Heavy Metals. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, July 2019. http://dx.doi.org/10.7546/crabs.2019.07.08.

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Shaw, George M. Genetic Variation of HIV: Viral Load and Genotypic Diversity in Relation to Viral Pathogenesis and Treatment. Fort Belvoir, VA: Defense Technical Information Center, January 1992. http://dx.doi.org/10.21236/ada246409.

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Harms, Nathan, Judy Shearer, James Cronin, and John Gaskin. Geographic and genetic variation in susceptibility of Butomus umbellatus to foliar fungal pathogens. Engineer Research and Development Center (U.S.), August 2021. http://dx.doi.org/10.21079/11681/41662.

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Large-scale patterns of plant invasions may reflect regional heterogeneity in biotic and abiotic factors and genetic variation within and between invading populations. Having information on how effects of biotic resistance vary spatially can be especially important when implementing biological control because introduced agents may have different Impacts through interactions with host-plant genotype, local environment, or other novel enemies. We conducted a series of field surveys and laboratory studies to determine whether there was evidence of biotic resistance, as foliar fungal pathogens, in two introduced genotypes (triploid G1, diploid G4) of the Eurasian wetland weed, Butomus umbellatus L. in the USA. We tested whether genotypes differed in disease attack and whether spatial patterns in disease incidence were related to geographic location or climate for either genotype. After accounting for location (latitude, climate), G1 plants had lower disease incidence than G4 plants in the field (38% vs. 70%) but similar pathogen richness. In contrast, bioassays revealed G1 plants consistently received a higher damage score and had larger leaf lesions regardless of pathogen. These results demonstrate that two widespread B. umbellatus genotypes exhibit different susceptibility to pathogens and effectiveness of pathogen biological controls may depend on local conditions.
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Amzeri, Achmad, B. S. DARYONO, and M. SYAFII. GENOTYPE BY ENVIRONMENT AND STABILITY ANALYSES OF DRYLAND MAIZE HYBRIDS. SABRAO Journal of Breeding and Genetics, September 2020. http://dx.doi.org/10.21107/amzeri.2020.2.

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The phenotypic analysis of new candidate varieties at multiple locations could provide information on the stability of their genotypes. We evaluated the stability of 11 maize hybrid candidates in five districts in East Java Province, Indonesia. Maize hybrids with high yield potential and early maturity traits derived from a diallel cross were planted in a randomized complete block design with two checks (Srikandi Kuning and BISI-2) as a single factor with four replicates. The observed traits were grain yield per hectare and harvest age. The effects of environment, genotype, and genotype × environment interaction on yield were highly significant (P < 0.01). KTM-1, KTM-2, KTM-4, KTM-5, and KTM-6 showed higher average grain yield per hectare than the checks (Srikandi Kuning = 8.49 ton ha−1 and BISI-2 = 7.32 ton ha−1) at five different locations. The average harvest age of 11 candidates was less than 100 days. KTM-4 and KTM-5 had production yields that were higher than the average yield of all genotypes in all environments (Yi > 7.78 tons ha−1) and were considered stable on the basis of three stability parameters, i.e., Finlay–Wilkinson, Eberhart–Russell, and additive main effect multiplicative interaction (AMMI). KTM-2 had the highest yield among all tested genotypes (9.33 ton ha−1) and was considered as stable on the basis of AMMI but not on the basis of Finlay–Wilkinson and Eberhart–Russell. KTM-1 performed well only in Pamekasan, whereas KTM-6 performed well only in Sampang. Thus, these two genotypes could be targeted for these specific locations.
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Velsko, S. Resolution in forensic microbial genotyping. Office of Scientific and Technical Information (OSTI), August 2005. http://dx.doi.org/10.2172/883607.

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Hinckley, Aubree Christine. Genotyping and Bioforensics of Ricinus communis. Office of Scientific and Technical Information (OSTI), January 2006. http://dx.doi.org/10.2172/900882.

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Fernando, Rohan L., Jack C. M. Dekkers, and Dorian J. Garrick. Bayesian Methods for Genomic Prediction and Genome-Wide Association Studies combining Information on Genotyped and Non-Genotyped Individuals. Ames (Iowa): Iowa State University, January 2014. http://dx.doi.org/10.31274/ans_air-180814-1245.

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Gao, David. A new sieving matrix for DNA sequencing, genotyping and mutation detection and high-throughput genotyping with a 96-capillary array system. Office of Scientific and Technical Information (OSTI), November 1999. http://dx.doi.org/10.2172/754779.

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Hultman, Keith, and Eve Mellgren. Fetching SNPs: A Dog Genotyping Laboratory for Undergraduate Biology. Genetics Society of America Peer-Reviewed Education Portal, September 2014. http://dx.doi.org/10.1534/gsaprep.2014.001.

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