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Dissertations / Theses on the topic 'Genomic techniques'

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1

Haghighi, Maryam. "Application of Combinatorial Optimization Techniques in Genomic Median Problems." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/20484.

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Constructing the genomic median of several given genomes is crucial in developing evolutionary trees, since the genomic median provides an estimate for the ordering of the genes in a common ancestor of the given genomes. This is due to the fact that the content of DNA molecules is often similar, but the difference is mainly in the order in which the genes appear in various genomes. The mutations that affect this ordering are called genome rearrangements, and many structural differences between genomes can be studied using genome rearrangements. In this thesis our main focus is on applying com
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Khanam, Taslima. "Sex determination and genetic management in Nile tilapia using genomic techniques." Thesis, University of Stirling, 2017. http://hdl.handle.net/1893/25285.

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The PhD research studied two aspects in tilapia, firstly the analysis of sex determination in Nile tilapia (evidence of complex sex-determining systems) and secondly the genetic management of the tilapia species, using different genomic analysis approaches. This research started with the development of two techniques: minimally invasive DNA sampling from fish mucus, which was found to be suitable for standard genotyping and double-digest restriction-site associated DNA sequencing – ddRADseq; and pre-extraction pooling of tissue samples for ddRADseq (BSA-ddRADseq), which was found to be suitabl
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Raiford, Douglas W. III. "Algorithmic Techniques Employed in the Isolation of Codon Usage Biases in Prokaryotic Genomes." Wright State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=wright1211902424.

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Brown, Margaret M. "Application of genomic techniques to development of biomarkers for the aquatic environment." Thesis, Glasgow Caledonian University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.443169.

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Sharma, Jason P. (Jason Poonam) 1979. "Classification performance of support vector machines on genomic data utilizing feature space selection techniques." Thesis, Massachusetts Institute of Technology, 2002. http://hdl.handle.net/1721.1/87830.

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Renard, Meseguer Joan. "Identification of genes related to seed longevity in Arabidopsis thaliana using genomic molecular techniques." Doctoral thesis, Universitat Politècnica de València, 2021. http://hdl.handle.net/10251/170554.

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Tesis por compendio<br>[ES] La longevidad de las semillas, o el tiempo durante el cual permanecen las semillas viables, es de gran importancia para la conservación de la biodiversidad, la agricultura y la economía. Además, el estudio de este parámetro puede contribuir a conocer mejor los mecanismos moleculares comunes a todos los organismos para prevenir el envejecimiento. Una de las principales estrategias de las semillas para ralentizar su envejecimiento consiste en detener su metabolismo, a través de su deshidratación. Otros mecanismos moleculares para evitar daños son el aislamiento frente
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Hinojosa, Galisteo Joan Carles 1993. "Exploring the butterfly speciation continuum : A study on butterfly speciation in the transition to genomic techniques." Doctoral thesis, Universitat Pompeu Fabra, 2021. http://hdl.handle.net/10803/672348.

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Butterflies are among the best studied animals, but despite the research efforts carried out during centuries, our knowledge on their diversity and on the mechanisms generating it is still incomplete. In order to understand how butterflies diversify, the speciation continuum of six study cases was examined using morphometrics and several genetic techniques (from sequencing specific markers to genomics). The analysis of phenotypic and genetic variation combined with gene flow evidence allowed to identify the states of the speciation continuum, i.e. to study the relationships between popul
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Padmanabhan, Babu roshan. "Taxano-genomics, a strategy incorporating genomic data into the taxonomic description of human bacteria." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM5056.

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Mon projet de doctorat était de créer un pipeline pour taxono-génomique pour la comparaison de plusieurs génomes bactériens. Deuxièmement, je automatisé le processus d'assemblage (NGS) et annotation à l'aide de divers logiciels open source ainsi que la création de scripts de maison pour le laboratoire. Enfin, nous avons intégré le pipeline dans la description de plusieurs espèces bactériennes de laboratoire sur. Cette thèse est divisée principalement en Taxono- génomique et Microbiogenomics. Les avis de la section taxono-génomique, décrit sur les avancées technologiques en génomique et métagén
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Tanov, Emil Pavlov. "The identification of biologically important secondary structures in disease-causing RNA viruses." University of the Western Cape, 2012. http://hdl.handle.net/11394/4562.

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Magister Scientiae - MSc<br>Viral genomes consist of either deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). The viral RNA molecules are responsible for two functions, firstly, their sequences contain the genetic code, which encodes the viral proteins, and secondly, they may form structural elements important in the regulation of the viral life-cycle. Using a host of computational and bioinformatics techniques we investigated how predicted secondary structure may influence the evolutionary dynamics of a group of single-stranded RNA viruses from the Picornaviridae family. We detected sign
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Visser, Johan Christiaan. "A study of genomic variation in and the development of detection techniques for potato virus Y in South Africa." Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/21878.

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Thesis (MSc)--University of Stellenbosch, 2008.<br>ENGLISH ABSTRACT: Potato virus Y (PVY) is responsible for considerable yield losses in the South African potato industry. The incidence of this virus has greatly increased over the past few years. Even more worrying is the variation of symptoms observed during PVY infection and the recent appearance of the more virulent PVYNTN strain in local fields. This project aimed to investigate the possible genetic variation within the viral genome and to establish the origin of strains. The project also aimed to establish a dependable, area specifi
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Palanca-Wessels, Maria Corinna. "In vitro analysis of cultured Barrett's esophagus cells : insights into mechanisms of genomic instability and possible therapeutic strategies /." Thesis, Connect to this title online; UW restricted, 1999. http://hdl.handle.net/1773/4995.

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SANTO, DEBORA. "The application of reduced-representation sequencing techniques for studying the structure of plant populations: A case study in common bean (Phaseolus vulgaris L.)." Doctoral thesis, Università Politecnica delle Marche, 2018. http://hdl.handle.net/11566/256205.

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The dissertation is comprised of two chapters. The first chapter is a literature review on the application of the two of the most frequently used reduced-representation sequencing techniques: RADseq (RAD sequencing; Restriction-site Associated DNA-sequencing) and GBS (Genotyping-by-Sequencing). Both techniques are based on the use of restriction enzymes and were developed primarily for genome-wide discovery of single-nucleotide polymorphism (SNP) markers. These methods have already been used in hundreds of studies to investigate the genetic diversity of large numbers of individuals under lower
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MONTEMURRO, MARILISA. "Algorithms for cancer genome data analysis - Learning techniques for ITH modeling and gene fusion classification." Doctoral thesis, Politecnico di Torino, 2022. http://hdl.handle.net/11583/2970978.

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Mlaga, Kodjovi Dodji. "Real-time genomics to decipher atypical bacteria in clinical microbiology." Thesis, Aix-Marseille, 2017. http://www.theses.fr/2017AIXM0594/document.

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L'objectif de notre thèse est d'appliquer la génomique en temps réel pour déchiffrer les caractéristiques génomiques bactériennes et les événements de recombinaison du génome des bactéries atypiques ainsi que leur impact sur les maladies infectieuses. Au cours de ma thèse, nous avons effectué une revue sur les outils bioinformatiques les plus courants utilisés en microbiologie clinique et mis en évidence l’impact de la recombinaison sur le comportement des bacteries. Le deuxième projet de notre thèse est de déchiffrer une epidémis de Staphylococcus saprophyticus causant des infections urinaire
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Soulier, Alexandra. "Défis techniques, problèmes éthiques : repenser l'éthique de la recherche en génomique humaine à l'ère des infrastructures de recherche." Thesis, Toulouse 3, 2017. http://www.theses.fr/2017TOU30177/document.

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Dans le champ de la recherche en génomique, comme dans d'autres domaines très informatisés, les bases de données et les biobanques sont organisées en infrastructures. Ce nouveau modèle organisationnel doit permettre de soutenir l'effort technique et collaboratif requis pour traiter des Big Data, c'est-à-dire des jeux de données trop volumineux et complexes pour être traités en utilisant les méthodes classiques. L'établissement de ces nouveaux environnements constitue un véritable défi technique et philosophique. Il requiert, pour être opérationnel, des cadres réglementaires adaptés, ouverts à
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Carvalho, Rafael Arrabaça de. "Avaliação do método de sequenciamento de nova geração no diagnóstico genético de neoplasia endócrina múltipla tipo 1." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-10012017-111320/.

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A neoplasia endócrina múltipla tipo 1 (NEM1) é uma doença genética, de herança autossômica dominante, caracterizada pelo desenvolvimento de tumores endócrinos acometendo, principalmente, hipófise, paratireoide e pâncreas/duodeno endócrinos. É causada, principalmente, por mutação germinativa no gene supressor tumoral MEN1 (11q13). A tumorigênese segue o modelo de Knudson (1971). O diagnóstico genético de famílias com NEM1 reconhece os portadores assintomáticos de mutação MEN1, permite o diagnóstico e tratamento precoce de tumores, promove a redução da morbimortalidade relacionada à NEM1 e exclu
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Smalley, Joshua Luke. "Refining and implementing the genomic analysis technique of connectivity mapping." Thesis, University of Leicester, 2013. http://hdl.handle.net/2381/27919.

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Microarray analysis measures the expression of thousands of mRNAs simultaneously, thereby creating genome-wide transcriptional snapshots of biological states. The novel technique of connectivity mapping is a tool that identifies functional chemical similarities by identifying similarities in the transcriptional signatures they induce. As this is a relatively new technique, the aims of this work were two-fold: Firstly, to optimise the method of connectivity mapping, and secondly to establish its potential uses, by applying the technique in several model systems. Connectivity mapping currently r
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Kruczkiewicz, Peter. "A comparative genomic framework for the in silico design and assessment of molecular typing methods using whole-genome sequence data with application to Listeria monocytogenes." Thesis, Lethbridge, Alta. : University of Lethbridge, Dept. of Biological Sciences, c2013, 2013. http://hdl.handle.net/10133/3391.

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Although increased genome sequencing e orts have increased our understanding of genomic variability within many bacterial species, there has been limited application of this knowledge towards assessing current molecular typing methods and developing novel molecular typing methods. This thesis reports a novel in silico comparative genomic framework where the performance of typing methods is assessed on the basis of the discriminatory power of the method as well as the concordance of the method with a whole-genome phylogeny. Using this framework, we designed a comparative genomic ngerprinting (
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Glaab, Enrico. "Analysing functional genomics data using novel ensemble, consensus and data fusion techniques." Thesis, University of Nottingham, 2011. http://eprints.nottingham.ac.uk/12727/.

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Motivation: A rapid technological development in the biosciences and in computer science in the last decade has enabled the analysis of high-dimensional biological datasets on standard desktop computers. However, in spite of these technical advances, common properties of the new high-throughput experimental data, like small sample sizes in relation to the number of features, high noise levels and outliers, also pose novel challenges. Ensemble and consensus machine learning techniques and data integration methods can alleviate these issues, but often provide overly complex models which lack gen
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Sheridan, Paul O. "Genetic analysis and manipulation techniques for dominant butyrate-producing bacteria of the human intestinal microbiota." Thesis, University of Aberdeen, 2014. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=223921.

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Genome sequencing of a large number Firmicute species has recently been completed, including some of the highly oxygen-sensitive butyrate-producing bacteria, belonging to the Lachnospiraceae and Ruminococcaceae families, which have been isolated at the Rowett Institute of Nutrition and Health. However, detailed knowledge of the biochemistry and physiology of these bacteria has been limited by a lack of detailed genomic annotation and pathway analysis, and lack of genetic manipulation techniques. Therefore, the aim of this work was the genomic analysis of the carbohydrate-utilisation and motili
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Fortune, Mary Doris. "Statistical techniques to fine map the related genetic aetiology of autoimmune diseases." Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/264764.

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Genome Wide Association Studies (GWAS) have uncovered many genetic regions which are associated with autoimmune disease risk. In this thesis, I present methods which I have developed to build upon these studies and enable the analysis of the causal variants of these diseases. Colocalization methods disentangle whether potential causal variants are shared or distinct in related diseases, and enable the discovery of novel associations below the single-trait significance threshold. However, existing approaches require independent datasets to accomplish this. I extended two methods to allow for th
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Elmouelhi, Ahmed (Ahmed M. ). 1979. "Genome scanning : an AFM-based DNA sequencing technique." Thesis, Massachusetts Institute of Technology, 2003. http://hdl.handle.net/1721.1/34149.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2003.<br>Includes bibliographical references (p. 157-160).<br>Genome Scanning is a powerful new technique for DNA sequencing. The method presented in this thesis uses an atomic force microscope with a functionalized cantilever tip to sequence single stranded DNA immobilized to a mica surface. The functionalized cantilever tip hybridizes with only one base type (A, C, T, or G) and results in distinct peaks in the AFM-produced image. Genome Scanning has been successful at identifying 40 base strands of synthes
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McKie, Arthur Bennett. "Alu-polymerase chain reaction genomic fingerprinting : a technique to identify novel lesions in pancreatic cancer." Thesis, Imperial College London, 1999. http://hdl.handle.net/10044/1/8702.

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Dickinson, Eleanor. "Characterising disordered proteins of the cancer genome using biophysical techniques." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/characterising-disordered-proteins-of-the-cancer-genome-using-biophysical-techniques(e7b59c5c-293c-4d49-b1cf-cb4c0bf2ccd3).html.

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Protein function and dysfunction, and their intimate ties to protein structure, has been a core focus of research for several decades. More recently, research into the lack of structure in proteins has reached fever pitch. Intrinsically disordered proteins (IDPs) are proteins (or protein regions) that exist as collapsed or extended, dynamically mobile conformational ensembles, either at secondary or tertiary level, whilst remaining biologically active. The properties of IDPs can impede their study; they are often inherently unstable, are vastly wide-ranging in molecular weight and often diffic
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Burns, Paul D. "Gene finding in eukaryotic genomes using external information and machine learning techniques." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/49023.

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Gene finding in eukaryotic genomes is an essential part of a comprehensive approach to modern systems biology. Most methods developed in the past rely on a combination of computational prediction and external information about gene structures from transcript sequences and comparative genomics. In the past, external sequence information consisted of a combination of full-length cDNA and expressed sequence tag (EST) sequences. Much improvement in prediction of genes and gene isoforms is promised by availability of RNA-seq data. However, productive use of RNA-seq for gene prediction has been diff
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Piper, Michael Bruce. "The Cryptosporidium parvum genome project and new techniques in HAPPY mapping." Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624351.

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Copin, Marie-Pierre. "Contribution au developpement de techniques pour l'etude du genome de frankia." Toulouse 3, 1986. http://www.theses.fr/1986TOU30152.

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Sánchez, Castro Marta. "Génétique des malformations cardiaques congénitales : apport de la technique de puces à ADN génomique (Comparative Genomic Hybridisation ; aCGH)." Nantes, 2014. https://archive.bu.univ-nantes.fr/pollux/show/show?id=05592c2e-47c9-4945-b913-3119094e970d.

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Notre projet a consisté à analyser par aCGH 316 patients présentant une malformation cardiaque congénitale (MCC)à type de transposition des gros vaisseaux, tétralogie de Fallot ou coarctation de l'aorte avec pour objectif la détection de microdélétions et microduplications génomiques et ainsi l'identification de nouveaux gènes contribuant à ces MCC. L'identification de nouveaux gènes permet une meilleure compréhension des mécanismes moléculaires et embryologiques à l'origine de ces malformations et un meilleur conseil génétique. Notre étude a conduit à l'identification de 21 microdélétions et
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Widén, Frederik. "Porcine cytomegalovirus : studies on the viral genome and development of novel diagnostic techniques /." Uppsala : Dept. of Veterinary Microbiology, Swedish Univ. of Agricultural Sciences ([Institutionen för veterinärmedicinsk mikrobiologi], Sveriges lantbruksuniv, 2002. http://epsilon.slu.se/avh/2002/91-576-6388-2.pdf.

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Johnson, Matthew David. "Understanding the regulation of acid resistance in E. coli using whole genome techniques." Thesis, University of Birmingham, 2011. http://etheses.bham.ac.uk//id/eprint/3006/.

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The ability of bacteria to thrive in a variety of host environments depends on their capacity to sense and respond to a wide array of stressors. E. coli encounters many stresses during transit through the gastro-intestinal tract, including acid stress. Acid stress response in E. coli is regulated by a complex network called AR2. The AR2 network comprises several local regulators that collate signals from multiple two-component systems (TCS) including RcsBD, EvgAS and PhoPQ. We combined lab-based evolution and whole genome re-sequencing to generate and identify mutations that confer increased a
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Parida, Mrutyunjaya. "Exploring and analyzing omics using bioinformatics tools and techniques." Diss., University of Iowa, 2018. https://ir.uiowa.edu/etd/6244.

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During the Human Genome Project the first hundred billion bases were sequenced in four years, however, the second hundred billion bases were sequenced in four months (NHGRI, 2013). As efforts were made to improve every aspect of sequencing in this project, cost became inversely proportional to the speed (NHGRI, 2013). Human Genome Project ended in April 2003 but research in faster and cheaper ways to sequence the DNA is active to date (NHGRI, 2013). On the one hand, these advancements have allowed the convenient and unbiased generation and interrogation of a variety of omics datasets; on the o
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Aleksandra, Patić. "Značaj molekularne dijagnostike u dokazivanju virusnog gastrointestinalnog sindroma u Vojvodini." Phd thesis, Univerzitet u Novom Sadu, Medicinski fakultet u Novom Sadu, 2018. https://www.cris.uns.ac.rs/record.jsf?recordId=106859&source=NDLTD&language=en.

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Uvod: Virusni gastrointestinalni sindrom je aktuelni zdravstveni problem u celom svetu. To važi kako u razvijenim zemljama, tako i u zemljama u razvoju, a posebno u nerazvijenim zemljama, gde je drugi po redu uzrok mortaliteta. Nagli početak bolesti, praćen pojavom velikog broja tečnih stolica, mukom, povraćanjem, bolovima u stomaku, temperaturom, malaksalo&scaron;ću, ima za posledicu dehidrataciju. U svim starosnim grupama obolelih, a naročito kod sasvim male dece, starih i imunodeficitarnih osoba može da dođe do smrtnog ishoda, ukoliko se brzo ne postavi tačna etiolo&scaron;ka dijagnoza bole
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Jung, Young-Sang. "Rapid determination of protein structures in solution using NMR dipolar couplings." Doctoral thesis, [S.l.] : [s.n.], 2005. http://webdoc.sub.gwdg.de/diss/2005/jung/jung.pdf.

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Nyman, Joel. "Övning och kreativ process genom Paradise League." Thesis, Kungl. Musikhögskolan, Institutionen för jazz, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kmh:diva-3988.

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In this essay I present my creative input and development as a musician since fall 2020 and the compositions that has come out of it. Furthermore, I reflect on preparing these songs for my exam concert with my band Paradise League, as well as adding a trumpet synth called EVI (Electric Valve Instrument) to the instrumentation. Having experienced embouchure fatigue and a lack of stamina to follow through with longer sets, I am also pursuing a more effective technique. Hence, I analyze my practice methods and discuss my trumpet teachers, their tools and the effects thereof. Through the combinati
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Evans, Daniel T. "A SNP Microarray Analysis Pipeline Using Machine Learning Techniques." Ohio University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1289950347.

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Hjort, Amanda. "Könsroller och Härskartekniker i Twilight : (re)produktion av patriarkalgenusstrukturer genom smäktande kärlekshistoria?" Thesis, Södertörns högskola, Lärarutbildningen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-16516.

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This essay aims to describe and problematize gender roles and master suppression techniques in Stephenie Meyer’s Twilight Saga. This is done in order to enable me, in my future profession as a teacher, to start an emancipatory discussion in class where pupils can become conscious of different ways of reading the love story. I will use the following two research questions to fulfil the purpose: 1) investigate which gender roles that appears in the book’s main characters Bella and Edward and 2) which master suppression techniques that colours their relationship. To answer the questions gender th
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Ulenius, Martina, and Ruiz Ulrika Tranemyr. ""Jag påverkade henne och hon påverkade mig" : en studie av personliga erfarenheter av att genomgå en Tapas Acupressure Technique- behandling." Thesis, Ersta Sköndal University College, Department of Social Work, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:esh:diva-357.

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Frisell, Malin, and Rama Malki. "Att härska genom internkommunikation : En observationsstudie om kvinnliga ledare." Thesis, Högskolan i Gävle, Företagsekonomi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-32956.

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Titel: Att härska genom internkommunikation – En observationsstudie om kvinnliga ledare Nivå: Examensarbete på grundnivå i ämnet företagsekonomi Författare: Rama Malki och Malin Frisell Handledare: Monika Wallmon och Svante Brunåker Datum: 2020 - juni Syfte: Syftet med denna studie är att öka förståelsen för hur kvinnliga ledare använder och utsätts för härskartekniker, med fokus på internkommunikation. Metod: Denna studie är kvalitativ och utgår från en deduktiv ansats. Den empiriska insamlingen av data har genomförts med hjälp av fem ostrukturerade observationer som sedan har tolkats och ana
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Hu, Jinnan. "Exploring Genome Structure and Gene Regulation Related to Virulence in Fungal Phytopathogens Using Next Generation Sequencing Techniques." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1366213390.

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Löllgen, Ruth Mari Caroline. "Genome wide screening of loss of heterozygosity in human midgut carcinoid tumors with fluorescent technique." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972556702.

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Löllgen, Ruth Mari Caroline. "Genome-wide screening of loss of heterozygosity in human midgut carcinoid tumors with fluorescent technique." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2004. http://dx.doi.org/10.18452/15047.

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Hintergrund: Karzinoid-Tumoren des embryonalen Mitteldarms sind seltene intestinale neuroendokrine Tumoren, bei denen zum Zeitpunkt der Diagnose häufig Metastasen vorliegen. Im Gegensatz zu Karzinoiden des Vorderdarms und Respirationstraktes sind sie nicht mit der Multiplen Endokrinen Neoplasie Typ 1 (MEN1) vergesellschaftet. Die Mechanismen ihrer Tumorigenesis sind weitgehend unbekannt. Methoden: Tumorgewebe acht sporadischer, maligner Dünndarm-Karzinoide war Objekt dieser Studie über Verlust der Heterozygotie ("Loss Of Heterozygosity" (LOH)) mit 131 fluoreszierenden Mikrosatelliten. DNA Se
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Alveteg, Ellen. "Dra stråken till sin spets : En undersökning av extended techniques för viola genom instudering av Viola Spaces av Garth Knox." Thesis, Luleå tekniska universitet, Institutionen för konst, kommunikation och lärande, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-69373.

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Efter att ha gått en kurs i fri improvisation där jag fick utforska min violas möjligheter till att producera ljud, väcktes en nyfikenhet hos mig kring utökade speltekniker som kan förekomma i nutida konstmusik, så kallade extended techniques. Jag fick tips om etydsamlingen Viola Spaces av Garth Knox där olika extended techniques presenteras och bestämde mig för att studera in fyra av dem. De etyderna jag valde behandlar olika stråktekniker, nämligen sul ponticello, sul tasto, tremolo och olika sätt att dra stråken på. Under arbetets gång redogör jag för de svårigheter jag stötte på under min
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Greco, Claudia <1976&gt. "Genomic characterization of the italian wolf (Canis lupus): the genes involved in black coat colour determination and application of microarray technique for snps detection." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2009. http://amsdottorato.unibo.it/1729/1/GRECO__CLAUDIA_TESI.pdf.

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This study provides a comprehensive genetic overview on the endangered Italian wolf population. In particular, it focuses on two research lines. On one hand, we focalised on melanism in wolf in order to isolate a mutation related with black coat colour in canids. With several reported black individuals (an exception at European level), the Italian wolf population constituted a challenging research field posing many unanswered questions. As found in North American wolf, we reported that melanism in the Italian population is caused by a different melanocortin pathway component, the K locu
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Greco, Claudia <1976&gt. "Genomic characterization of the italian wolf (Canis lupus): the genes involved in black coat colour determination and application of microarray technique for snps detection." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2009. http://amsdottorato.unibo.it/1729/.

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Abstract:
This study provides a comprehensive genetic overview on the endangered Italian wolf population. In particular, it focuses on two research lines. On one hand, we focalised on melanism in wolf in order to isolate a mutation related with black coat colour in canids. With several reported black individuals (an exception at European level), the Italian wolf population constituted a challenging research field posing many unanswered questions. As found in North American wolf, we reported that melanism in the Italian population is caused by a different melanocortin pathway component, the K locu
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45

Monlun, Eric. "Diagnostic virologique des infections a papillomavirus de type 16 et 18 : mise au point d'une technique d'amplification genomique (pcr : [polymerase chain reaction])." Université Louis Pasteur (Strasbourg) (1971-2008), 1990. http://www.theses.fr/1990STR1M093.

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46

Zhu, Zeyu. "Multi-Omics Stress Responses and Adaptive Evolution in Pathogenic Bacteria: From Characterization Towards Diagnostic Prediction." Thesis, Boston College, 2020. http://hdl.handle.net/2345/bc-ir:108912.

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Thesis advisor: Tim van Opijnen<br>Thesis advisor: Welkin Johnson<br>Pathogenic bacteria can experience various stress factors during an infection including antibiotics and the host immune system. Whether a pathogen will establish an infection largely depends on its survival-success while enduring these stress factors. We reasoned that the ability to predict whether a pathogen will survive under and/or adapt to a stressful condition will provide great diagnostic and prognostic value. However, it is unknown what information is needed to enable such predictions. We hypothesized that under a stre
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47

Hooghvorst, Isidre. "Development of doubled haploids, chromosome doubling and CRISPR/Cas9 techniques in melon for the next generation of breeding." Doctoral thesis, Universitat de Barcelona, 2021. http://hdl.handle.net/10803/673451.

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The general aim of this PhD thesis was to study, develop and apply different breeding techniques in melon species in order to produce new commercial cultivars. The three specific objectives of this thesis are: 1. To study and evaluate the commercial value and the parthenogenetic capacity of seven genotypes of C. melo var. Inodorus “Piel de Sapo” type to obtain DH lines which might be further used as parental lines for commercial hybrid F1 seed production. The parthenogenetic generation of DHs from the seven genotypes was evaluated and optimized through the analysis and description of the di
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Pham, Hoang Son. "Nouvelles techniques d'extraction de motif pour l'étude d'association à l'échelle du génome." Thesis, Rennes 1, 2017. http://www.theses.fr/2017REN1S074/document.

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Les études d'association sur un génome complet (GWAS) sont conçues pour découvrir les combinaisons de points de polymorphisme (SNP) associées à des maladies. La découverte de ces associations permet d'élaborer de meilleures stratégies pour détecter, traiter ou prévenir les maladies. Récemment, l'utilisation de techniques d'extraction de patterns discriminatif a été investiguée dans le cadre de problématiques GWAS. Toutefois, la découverte de combinaisons de SNP dans de grands jeux de données GWAS est encore difficile à cause de la complexité des algorithmes utilisés. La thèse se propose donc d
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Kadner, Maxime. "Développements méthodologiques et applications en biologie translationnelle de techniques d'édition du génome chez le blé tendre." Electronic Thesis or Diss., Université Clermont Auvergne (2021-...), 2023. http://www.theses.fr/2023UCFA0029.

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Un défi majeur pour l'agriculture sera sa capacité à nourrir le monde du 21ème siècle face au changement climatique et à l'accroissement de la population, tout en répondant à la nécessité de la transition agroécologique. Le blé tendre (Triticum aestivum) en sera un enjeu clé, et les biotechnologies un outil clé à mobiliser. Trois axes de recherche mettant en jeu l'outil CRISPR-Cas ont été abordés dans cette thèse. Tout d'abord nous avons évalué l'utilisation d'enzymes alternatives à Cas9, en particulier pour leur capacité à réaliser des mutations multiples. Nous avons testé l'enzyme Cas12a, re
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St, John Oliver Tudor Lockhart. "Genome engineering and gene drive in the mosquito aedes aegypti." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:1251080e-cf7b-4bdd-b01e-d01748ead2d2.

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Genetic control strategies are a novel method for reducing populations of pest insects such as the yellow fever mosquito Aedes aegypti, a major vector of several important arboviral diseases. This thesis describes efforts to develop new tools to engineer the Ae. aegypti genome and to better understand existing tools, and furthermore to use these to engineer a gene drive system in Ae. aegypti. The piggyBac transposon was found to be extremely stable in the germline of Ae. aegypti, and transposons engineered into the germline could not be remobilized with either an endogenous or exogenous source
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