Relevant bibliographies by topics / Genetic markers; DNA

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Genetic markers; DNA'

Author: Grafiati
Published: 4 June 2021
Last updated: 3 February 2022

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Journal articles on the topic "Genetic markers; DNA"

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Chesnokov, Yu V. "GENETIC MARKERS: COMPARATIVE CLASSIFICATION OF MOLECULAR MARKERS." Vegetable crops of Russia, no. 3 (July 25, 2018): 11–15. http://dx.doi.org/10.18619/2072-9146-2018-3-11-15.

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With the creation of the molecular markers allowing to carry out analysis of genotypes on the level initial genetic information – DNA, onset one of the most multifarious and one of the most large in number class of markers at the present day. It is concerned with that each separate nucleic acid sequence is unique on its structure. Set of molecular and genetic methods, named as DNA-fingerprinting, most wide used in modern investigations for solving different problems in different biological areas. In this connection, necessity in comparative classification of modern molecular and genetic markers is actual. Based on published literature material it shown data on different classifications of molecular markers. Determined definition of term “marker” in genetics and breeding. Gave the characters and distinctive features of genetic markers. It given the definition what is “good” genetic marker as well as kinds, categories, variations and types on heredity of molecular markers. Manifested by means of molecular markers polymorphisms can classified on polymorphism of sequence itself (including nucleotide substitution and insertion-deletion) and polymorphism the number of tandem repeat sequences in repeated regions. Moreover, molecular markers can classify on two variations: anonymous, for which nucleotide acid sequence unknown and for manifestation of the molecular marker its detection not necessary (for example, RAPD, AFLP, RFLP), and announce (or determined), for which nucleic acid sequence is known or can be detect during analysis (for example, SNP, CAPS, STS). However, in independence on using of molecular markers the choice of method of investigation will be depend on investigated plant species as well. The next influence of molecular and genetic methods on genetics and practical breeding of plants will be depend on results, which will be obtain, in particular, on revealing the possibility or not possibility of genotyping of individual on single genetic marker as wel as on economic price of obtain informative data.
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Trent, Ronald J. "DNA markers in genetic disease." Medical Journal of Australia 152, no. 6 (March 1990): 281–82. http://dx.doi.org/10.5694/j.1326-5377.1990.tb120942.x.

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Nam, Vu Tuan, Pham Le Bich Hang, Nguyen Nhat Linh, Luu Han Ly, Huynh Thi Thu Hue, Nguyen Hai Ha, Ha Hong Hanh, and Le Thi Thu Hien. "Molecular markers for analysis of plant genetic diversity." Vietnam Journal of Biotechnology 18, no. 4 (May 24, 2021): 589–608. http://dx.doi.org/10.15625/1811-4989/18/4/15326.

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Genetic diversity plays an important role in diversity conservation at multiple levels and supports to monitor and assess genetic variation. In plants, genetic diversity provides the ability to adapt and respond to environmental conditions that helps plants to survive through changing environments. Genetic diversity analyses based on molecular genetic markers are effective tools for conservation and reintroduction of rare and endangered species. In recent years, the development of various chemical and molecular techniques for studying genetic diversity has received great attention. While biochemical markers are primarily used in the diagnosis of pathogens, DNA markers have been developed and widely applied for identification of species and population based on the genotype of an organism that is more stable and not easily affected by the environmental factors. PCR-based molecular marker tools, such as restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNA (RAPD), simple sequence repeats (SSRs) are used for analysing the difference in the targeted DNA sequences. With the rapid and robust development of genomic sequencing technology it is now possible to obtain and analyse DNA sequences of the whole genome of studied organisms. However, each type of DNA markers has different principles, as well as the pros and cons of specificity. In this article, we review methods and point out DNA markers, which are considered as reliable and widely used tools for the detection of genetic variation. In addition, we present the application of DNA marker in analysing genetic diversity of wild, domestic and medicinal plants, as well as some perspectives on the future of DNA marker’s application in the analysis of genetic diversity.
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Hallerman, Eric M., and Jacques S. Beckmann. "DNA-Level Polymorphism as a Tool in Fisheries Science." Canadian Journal of Fisheries and Aquatic Sciences 45, no. 6 (June 1, 1988): 1075–87. http://dx.doi.org/10.1139/f88-131.

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Several methods for the visualization of genetic polymorphisms at the nucleic acid level have been developed. Such polymorphisms promise to be exceedingly numerous, and may form the basis for a number of scientific and practical applications in fisheries science. An expanded number of genetic markers should increase the statistical power of marker-based studies in population genetics, for example, improving the sensitivity of biological stock assessments and of studies assessing the impact of stocking programs upon natural populations. Utilization of such genomic markers could contribute to the rapid elaboration of piscine genomic maps and to development of markers for health- and production-related traits in fishes.
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Hanáček, P., T. Vyhnánek, M. Rohrer, J. Cieslarová, and H. Stavělíková. "DNA polymorphism in genetic resources of red pepper using microsatellite markers." Horticultural Science 36, No. 4 (November 20, 2009): 127–32. http://dx.doi.org/10.17221/7/2009-hortsci.

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Genetic variability among 41 accessions of red pepper (<I>Capsicum annuum</I> L.) was assessed using eight microsatellite markers. Three of the microsatellite markers (<I>Hpms 1-1, Hpms 1-168, and Hpms 1-274</I>) had uniform spectra in all the analyzed plants. Two to eight alleles were detected for the remaining loci. In total, 28 alleles were detected, i.e. 3.5 alleles per one microsatellite locus on average. The highest number of different alleles was detected with <I>Hpms 1-5</I> (8 alleles) and <I>Hpms 2-21</I> primers (7 alleles). Molecular data were complemented with morphological measurements according to the descriptor list for the genus <I>Capsicum</I>. A dendrogram based on our genetic analysis suggests a high level of similarity between some of the accessions presumed to be distant and, at the same time, genetic variability between accessions of the same or similar name. These results show the possibility of duplicities in the current Czech collection of red pepper genetic resources.
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Teneva, A., and M. P. Petrovic. "Application of molecular markers in livestock improvement." Biotehnologija u stocarstvu 26, no. 3-4 (2010): 135–54. http://dx.doi.org/10.2298/bah1004135t.

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With recent developments in DNA technologies, a large number of genetic polymorphisms at DNA sequence level has been introduced over the last decades as named DNA-based markers. The discovery of new class of DNA profiling markers has facilitated the development of marker-based gene tags, mapbased cloning of livestock important genes, variability studies, phylogenetic analysis, synteny mapping, marker-assisted selection of favourable genotypes, etc. The most commonly used DNA-based markers have advantages over the traditional phenotypic and biochemical markers since they provide data that can be analyzed objectively. In this article the main applications of molecular markers in present-day breeding strategies for livestock improvement - parentage determination, genetic distance estimation, genetic diversity, gene mapping and marker-assisted selection have been reviewed.
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Stone, W. H., J. J. Ely, G. S. Manis, and J. L. VandeBerg. "Classical genetic markers and DNA markers: A commensal marriage." Primates 34, no. 3 (July 1993): 365–76. http://dx.doi.org/10.1007/bf02382632.

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Thompson, Paul G., Liang L. Hong, Kittipat Ukoskit, and Zhiqiang Zhu. "Genetic Linkage of Randomly Amplified Polymorphic DNA (RAPD) Markers in Sweetpotato." Journal of the American Society for Horticultural Science 122, no. 1 (January 1997): 79–82. http://dx.doi.org/10.21273/jashs.122.1.79.

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RAPD marker analyses were completed on parents and progeny of two sweetpotato [Ipomoea batatas (L.) Lam.] crosses to determine the feasibility of genetic linkage map construction. A total of 100 primers was tested and 96 produced amplified genomic DNA fragments. The average number of polymorphisms per primer was 0.69. A total of 134 polyphorphic markers was observed and 74 (60%) segregated 1 band present : 1 band absent as needed for use in genetic linkage mapping of polyploids. The 60% of RAPD markers that segregated 1:1 shows that genetic linkage mapping of the hexaploid sweetpotato by RAPD marker analysis is feasible. Linkage was determined for all markers that segregated 1:1 and five pairs of linked markers were found. These were the first linked molecular markers found in sweetpotato and they show that construction of a genetic linkage map is feasible. A genetic linkage map will be a valuable tool to assist in genetic improvements.
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Teneva, A., K. Dimitrov, Caro Petrovic, M. P. Petrovic, I. Dimitrova, N. Tyufekchiev, and N. Petrov. "Molecular genetics and SSR markers as a new practice in farm animal genomic analysis for breeding and control of disease disorders." Biotehnologija u stocarstvu 29, no. 3 (2013): 405–29. http://dx.doi.org/10.2298/bah1303405t.

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Molecular genetics investigates the genetic makeup of individuals at the DNA level. That includes the identification and mapping of molecular genetic markers and genetic polymorphisms. Molecular genetic markers (DNA markers) are one of the most powerful means for the genomic analysis and allow the connection of hereditary traits with genomic variation. Molecular marker technology has developed rapidly over the last decade and two shapes of specific DNA based marker, Simple Sequence Repeats (SSRs), also known as microsatellites, and Single Nucleotide Polymorphisms (SNPs) prevail applications in modern genetic analysis. Genomic simple sequence repeats (SSRs, microsatellites) have been used for a variety of purposes, including gene tagging, physical mapping, genome mapping, estimation of genetic diversity, phylogenetic and conservation genetic purposes in farm animal breeding. SSR analyses are applied successfully in parentage verification and pedigree analysis, as disease markers and to locate the mutation in genetic disorders in livestock animals. The ultimate use of SSRs markers is for mapping quantitative trait loci (QTL), marker assisted selection (MAS) in order to practice genomic selection and improve the farm animal health. Developments in ?omics? technologies, such as genomic selection, may help overcome several of the limitations of traditional breeding programmes and will be especially beneficial in breeding for lowly heritable disease traits that only manifest themselves following exposure to pathogens or environmental stressors in adulthood. The current paper provides a brief overview of the present - day application of microsatellites markers in animal breeding and make significant contribution to the overall farm animal health and resistance to disease.
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Toomey, Lola, Dirk Welsford, Sharon A. Appleyard, Andrea Polanowski, Cassandra Faux, Bruce E. Deagle, Mark Belchier, James Marthick, and Simon Jarman. "Genetic structure of Patagonian toothfish populations from otolith DNA." Antarctic Science 28, no. 5 (May 19, 2016): 347–60. http://dx.doi.org/10.1017/s0954102016000183.

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AbstractThe Patagonian toothfish, Dissostichus eleginoides, is a valuable fishery species and has a discontinuous distribution across the Southern Ocean. Identification of the genetic stock structure of toothfish would allow evaluation of the suitability of the spatial scale at which fisheries management operates. Genetic subdivision seems likely given the species distribution. Population genetics studies of this species have been performed; however, they have been limited by sample size, spatial coverage and/or the type of markers investigated. As a potential solution, we developed methods for extracting toothfish DNA from otoliths that are available in large numbers from collections held at several research institutes. Genetic differentiation between the three oceanic sectors was investigated. Four mitochondrial and four nuclear markers with multiple single nucleotide polymorphisms were sequenced by high throughput sequencing for samples from six locations. Genetic differentiation was found between three sectors with nuclear markers. However, only the Pacific sector was differentiated from other sectors with mitochondrial markers. This study demonstrates the usefulness of otolith DNA as a means of increasing sample sizes for population genetics research of fish. Additionally, the combination of nuclear and mitochondrial markers may allow insight into how the observed differences in movements between male and female toothfish impact population structure.
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Dissertations / Theses on the topic "Genetic markers; DNA"

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Carter, Deidre Anne. "DNA polymorphisms as genetic markers in Phytophthora infestans." Thesis, Imperial College London, 1991. http://hdl.handle.net/10044/1/46699.

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Laughlin, Thomas Fain. "Hypervariable DNA markers and population structure in three fish species." Diss., This resource online, 1993. http://scholar.lib.vt.edu/theses/available/etd-06062008-171854/.

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Valdman, Alexander. "Molecular genetic markers of prostate cancer development /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-618-9/.

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Silva, E. P. da. "Population genetic studies of the mussel Mytilus using nuclear DNA markers." Thesis, Swansea University, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.639035.

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The genetics of the mussel Mytilus is one of the most extensively studied among the marine invertebrates. This work is concerned with the use of nuclear DNA polymorphisms to study aspects of Mytilus population genetics. Sixteen populations of three species, M. edulis, M. galloprovincialis and M. trossulus were studied for 6 nDNA markers. SSCP analysis was used to enhance the variability in the N118 anonymous scnDNA region, a PCR fragment which previously showed little RFLP variation, and in a second PCR product from a nrDNA internal transcribed spacer region. In the first case, the number of alleles increased from 4 to 7 and the FST estimate from 0.0072 to 0.0096. In the second case, four alleles were resolved and a statistically significant FST value of 0.08 was revealed. Also, primers for an intron of myosin heavy chain was developed and fifteen alleles were resolved. Three of the alleles are M. trossulus specific, but the others show significant frequency differences among different species and even among populations in the same species. Analysis of the nuclear DNA population structure in M. edulis showed statistical significant levels of geographic variation, and when compared with allozymes revealed significant differences between the two sets of markers. This result is consistent with the operation of balancing selection on allozymes. Moreover, the use of the Ewens-Watterson homozygosity test revealed for the nDNA polymorphism a departure from a strictly neutral model, indicating that assumptions about the neutrality of a DNA polymorphisms could also be under suspicion. Evolutionary relationships between the three mussel species using nDNA markers are in line with morphological and allozymes studies, which show M. edulis as more closely related to M. galloprovincialis than to M. trossulus. However, a much higher level of differentiation than previously reported was found between the three taxa.
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Syaifudin, Mochamad. "Species-specific DNA markers for improving the genetic management of tilapia." Thesis, University of Stirling, 2015. http://hdl.handle.net/1893/22624.

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The tilapias are a group of African and Middle Eastern cichlid fish that are widely cultured in developed and developing countries. With many different species and sub-species, and extensive use of interspecies hybrids, identification of tilapia species is of importance in aquaculture and in wild populations where introductions occur. This research set out to distinguish between tilapia species and sub-species by retrieving species-specific nuclear DNA markers (SNPs) using two approaches: (i) sequencing of the coding regions of the ADA gene; and (ii) next-generation sequencing, both standard RADseq and double-digest RADseq (ddRADseq). The mitochondrial DNA (mtDNA) marker cytochrome c oxidase subunit I (COI) was used to verify tilapia species status. ADA gene sequence analysis was partially successful, generating SNP markers that distinguished some species pairs. Most species could also be discriminated using the COI sequence. Reference based analysis (RBA: using only markers found in the O. niloticus genome sequence) of standard RADseq data identified 1,613 SNPs in 1,002 shared RAD loci among seven species. De novo based analysis (DBA: based on the entire data set) identified 1,358 SNPs in 825 loci and RBA detected 938 SNPs in 571 shared RAD loci from ddRADseq among 10 species. Phylogenetic trees based on shared SNP markers indicated similar patterns to most prior phylogenies based on other characteristics. The standard RADseq detected 677 species-specific SNP markers from the entire data set (seven species), while the ddRADseq retrieved 38 (among ten species). Furthermore, 37 such SNP markers were identified from ddRADseq data from a subset of four economically important species which are often involved in hybridization in aquaculture, and larger numbers of SNP markers distinguished between species pairs in this group. In summary, these SNPs are a valuable resource in further investigating hybridization and introgression in a range of captive and wild stocks of tilapias.
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Bishop, Alexander James Roy. "The dynamics of minisatellite changes during meiosis in the yeast Saccharomyces cerevisiae." Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339351.

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Prodöhl, Paulo A. "Multilocus and single locus minisatellite DNA polymorphism in brown trout (Salmo trutta L.) populations." Thesis, Queen's University Belfast, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296801.

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Bentley, Elizabeth. "Genetic analysis of the myelencephalic blebs mutation on mouse chromosome." Thesis, University College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265754.

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Honing, Jennifer. "Evaluation and implementation of DNA-based diagnostic methodology to distinguish wheat genotypes." Thesis, Link to the online version, 2007. http://hdl.handle.net/10019/638.

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Kremer, Kristin. "Genetic markers for Mycobacterium tuberculosis characterization and spread of the Beijing genotype /." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2005. http://dare.uva.nl/document/78818.

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Books on the topic "Genetic markers; DNA"

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NATO Advanced Research Workshop on DNA Polymorphisms as Disease Markers (1990 London, England). DNA polymorphisms as disease markers. New York: Plenum Press, 1991.

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Elles, Robert George. Recombinant DNA probes as markers for genetic disease. Manchester: University ofManchester, 1994.

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Nelson, Alondra. Genetics and the unsettled past: The collision of DNA, race, and history. New Brunswick, NJ: Rutgers University Press, 2012.

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Butler, John M. Improved analysis of DNA short tandem repeats with time-of-flight mass spectrometry. Washington, D.C: U.S. Dept. of Justice, Office of Justice Programs, National Institute of Justice, 2001.

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Butler, John M. Improved analysis of DNA short tandem repeats with time-of-flight mass spectrometry: Science and technology research report. Washington, DC: U.S. Dept. of Justice, Office of Justice Programs, National Institute of Justice, 2001.

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E, Lindsten Jan, Pettersson Ulf, Nobelstiftelsen, and Alfred Nobel's Björkborn Foundation, eds. Etiology of human disease at the DNA level. New York: Raven Press, 1991.

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Klimenko, Irina, Nikolay Kozlov, Sergey Kostenko, Anastasia Shamustakimova, and Yulian Mavlyutov. Identification and certification of forage grasses (meadow clover, alfalfa, sowing and hop) based on DNA markers. ru: Federal Williams Research Center of Forage Production and Agroecology, 2020. http://dx.doi.org/10.33814/978-5-6043194-9-9.

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A technology has been developed for DNA identification and certification of varieties of meadow clover (Trifolium pratense L.), alfalfa (Medicago varia Mart.), Sowing (M. sativa L.) and hop (M. lupuli-na L.) based on molecular analysis with using SSR and SRAP markers. The recommendations contain a description of the sequence of experiments and protocols for DNA typing procedures. The presented methods were developed by the authors on the basis of their own experimental research and using the data available in the literature. A characteristic of informative primers for each marking system is given, a set of DNA identification markers is proposed, and unique molecular genetic formulas of varieties are drawn up as the basis for a reference genetic passport. Methodological recommendations were prepared with the aim of mastering the technology of DNA certification of forage grasses in practice. Designed for managers and specialists of research and control laboratories, can serve as a textbook for students and postgraduates in specialized specialties.
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Single nucleotide polymorphisms: Methods and protocols. 2nd ed. New York: Humana, 2009.

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DNA barcodes: Methods and protocols. New York: Humana Press, 2012.

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Crop breeding: Methods and protocols. New York: Humana Press, 2014.

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Book chapters on the topic "Genetic markers; DNA"

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Gusella, J. F. "DNA Markers in Huntington’s Disease." In Genetic Engineering: Principles and Methods, 333–47. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4973-0_15.

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Motulsky, Arno G. "Genetic Approaches to Common Diseases." In DNA Polymorphisms as Disease Markers, 1–4. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3690-1_1.

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Kearney, Phillip, and Jonathan Wolfe. "Centromeric Alphoid DNA on the Y Chromosome." In Genetic Markers of Sex Differentiation, 139–48. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4899-1965-6_12.

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Chamberlain, J. C., and D. J. Galton. "Atherosclerosis: The Genetic Analysis of a Multi-Factorial Disease." In DNA Polymorphisms as Disease Markers, 123–33. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3690-1_12.

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Havekes, Louis M., Arn M. J. M. van den Maagdenberg, Peter de Knijff, Monique Mulder, and Rune R. Frants. "Apolipoprotein E Polymorphisms and the Genetic Heterogeneity of Familial Dysbetalipoproteinemia." In DNA Polymorphisms as Disease Markers, 71–77. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3690-1_7.

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Kim, Se-Kwon. "Genetic Diversity and DNA Markers in Fish." In Essentials of Marine Biotechnology, 109–44. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-20944-5_5.

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Madhusudhana, R. "Application of DNA Markers for Genetic Improvement." In Sorghum Molecular Breeding, 71–99. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2422-8_4.

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Permutt, M. Alan, Laszlo Koranyi, and Akira Matsutani. "Molecular Genetic Approach to Polygenic Disease: Non-Insulin Dependent Diabetes Mellitus (NIDDM)." In DNA Polymorphisms as Disease Markers, 43–59. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3690-1_5.

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Mallet, J., A. Malafosse, M. Leboyer, T. d’Amato, S. Amadéo, M. Abbar, M. C. Babron, et al. "Family Studies of Affective Disorders with 11p15.5 DNA Markers." In Genetic Research in Psychiatry, 164–72. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-46762-2_14.

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Ott, Jurg. "Estimating Crossover Frequencies and Testing for Numerical Interference with Highly Polymorphic Markers." In Genetic Mapping and DNA Sequencing, 49–63. New York, NY: Springer New York, 1996. http://dx.doi.org/10.1007/978-1-4612-0751-1_4.

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Conference papers on the topic "Genetic markers; DNA"

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"Breeding of potato resistant to late blight using genetic resources and DNA markers." In Current Challenges in Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk State University, 2019. http://dx.doi.org/10.18699/icg-plantgen2019-69.

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Liu, Haiying, Guie Wang, Xiangying Meng, and Xiuli Wang. "Genetic Variation in Six Oratosquilla oratoria Populations Revealed by Random Amplified Polymorphic DNA (RAPD) Markers." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5516354.

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Gumilar, Gun Gun, Yunita Purnamasari, and Rahmat Setiadi. "Mitochondrial DNA variant at HVI region as a candidate of genetic markers of type 2 diabetes." In PROCEEDINGS OF INTERNATIONAL SEMINAR ON MATHEMATICS, SCIENCE, AND COMPUTER SCIENCE EDUCATION (MSCEIS 2015). AIP Publishing LLC, 2016. http://dx.doi.org/10.1063/1.4941154.

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"Postgenomic technologies in practical forestry: development of DNA markers and population genetic databases for timber origin identification, genetic monitoring, breeding and other applications." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-093.

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Golovatskaya, A. V., and S. Z. Guchetl. "THE CERTIFICATION OF SUNFLOWER LINES FROM THE COLLECTION OF THE DON EXPERIMENTAL STATION OF V.S. PUSTOVOIT ALL-RUSSIAN RESEARCH INSTITUTE OF OIL CROPS BY USING DNA MARKERS." In 11-я Всероссийская конференция молодых учёных и специалистов «Актуальные вопросы биологии, селекции, технологии возделывания и переработки сельскохозяйственных культур». V.S. Pustovoit All-Russian Research Institute of Oil Crops, 2021. http://dx.doi.org/10.25230/conf11-2021-39-43.

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The aim of this research was to develop molecular genetic passports of sunflower lines from the collection of the Don experimental station of V.S. Pustovoit All-Russian Research Institute of Oil Crops based on polymorphic fractions of microsatellite DNA. We used 17 lines as a research material. We used 12 pairs of primers for genotyping. We found that the ORS 559 locus was monomorphic for these samples. The rest of the loci had from 2 to 4 alleles. The average number of alleles per locus was 2.75, PIC – 0.49, the effective number of alleles – 2.16. The analysis of the DNA profiles of the lines showed the individuality of the allelic composition of each of them. The analysis of the genetic relations between the lines showed that the studied lines were divided into two groups, with a genetic distance between them of 5.9.
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Jain, Surbhi, Jamin D. Steffen, Yih-Ping Su, Jeremy Wang, Ting-Tsung Chang, Chi-Tan Hu, Wei Song, and Ying-Hsiu Su. "Abstract 5699: Detection of genetic and epigenetic DNA markers in urine for the early detection of liver cancer." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-5699.

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Engler, David, Sumeet Gupta, Whitfield Growdan, Ronny Drapkin, Mai Nitta, Petra Sergent, Serena Allred, et al. "Abstract 5093: Genome wide DNA copy number analysis of serous type ovarian carcinomas identifies genetic markers predictive of clinical outcome." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-5093.

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Jain, Surbhi, Hie-Won Hann, Sitong Chen, Selena Y. Lin, Ting-Tsung Chang, Chi-Tan Hu, Wei Song, and Ying-Hsiu Su. "Abstract 3128: Detection of genetic and epigenetic DNA markers in urine for the early detection of primary and recurrent HCC." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-3128.

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Savichenko, V. G., and S. A. Ramazanova. "THE IDENTIFICATION OF SOYBEAN VARIETIES OF THE BREEDING OF V.S. PUSTOVOIT ALL-RUSSIAN RESEARCH INSTITUTE OF OIL CROPS BY MICROSATELLITE ANALYSIS." In 11-я Всероссийская конференция молодых учёных и специалистов «Актуальные вопросы биологии, селекции, технологии возделывания и переработки сельскохозяйственных культур». V.S. Pustovoit All-Russian Research Institute of Oil Crops, 2021. http://dx.doi.org/10.25230/conf11-2021-97-101.

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The identification of breeding material and certification of varieties is of great importance for the protection of the copyright of breeders. Microsatellite DNA loci are effectively used for these purposes. The aim of the research was to identify the soybean varieties of the breeding of V.S. Pustovoit All-Russian Research Institute of Oil Crops using the previously tested 12 microsatellite markers. As a result of research, we obtained the unique sets of alleles for eight varieties; two varieties had identical alleles. We divided all soybean genotypes into two large clusters. We observed the closest genetic relation between the varieties Duar and Kora (the Armavir experimental station of V.S. Pustovoit All-Russian Research Institute of Oil Crops. The resulting sets of alleles can be used to develop molecular genetic passports.
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Erickson, David, Xuezhu Liu, Roberto Venditti, Ulrich Krull, and Dongqing Li. "A DNA Hybridization Chip With Electrokinetically-Based Single Nucleotide Polymorphism (SNP) Discrimination." In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-59320.

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Biosensors and more specifically biochips exploit the interactions between a target analyte and an immobilized biological recognition element to produce a measurable signal. Systems based on surface phase nucleic acid hybridization, such as modern microarrays, are particularly attractive due to the high degree of selectivity in the binding interactions. In this work an electrokinetically controlled poly(dimethylsiloxane) based DNA hybridization microfluidic chip is presented. The electrokinetic delivery technique provides the ability to dispense controlled sample sizes to the hybridization array for quantitative analysis while serving to increase the mass transfer rate and therefore reduce the overall analysis time. An automatic, electrokinetically based, single-nucleotide polymorphism (SNP) discrimination technique (that takes advantage of the combined effects of joule heating, applied potential field and the shear gradients within the double layer field on the thermodynamic stability of the target: probe complex) will also be described for the first time. The clinical utility of the technique will be demonstrated through the detection of genetic markers associated with spinal muscular atrophy, specifically the common C←T mutation in the SMN1 gene.
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Reports on the topic "Genetic markers; DNA"

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Hoon, Dave S. Serum DNA Microsatellites as Surrogate Genetic Markers of Breast Cancer Progression. Fort Belvoir, VA: Defense Technical Information Center, April 2005. http://dx.doi.org/10.21236/ada442454.

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Hoon, Dave S. Serum DNA Microsatellites as Surrogate Genetic Markers of Breast Cancer Progression. Fort Belvoir, VA: Defense Technical Information Center, October 2002. http://dx.doi.org/10.21236/ada412196.

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Hoon, Dave S. Serum DNA Microsatellites as Surrogate Genetic Markers of Breast Cancer Progression. Fort Belvoir, VA: Defense Technical Information Center, October 2003. http://dx.doi.org/10.21236/ada424571.

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Amzeri, Achmad, Kaswan Badami, and Gita Pawana. Inheritance of resistance to downy mildew (Peronosclerospora maydis) in crossing of Madura Maize Plant (Zea mays L.). Innovative Scientific Information & Services Network, May 2019. http://dx.doi.org/10.21107/amzeri.2019.1.

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Hybridization of Back cross is one method to get varieties that are resistant to downy mildew. The purpose of this study was to obtain information on inheritance characteristics of downy mildew resistance. This research was conducted at the experiment center of Agro-Technology Study Program of Agriculture Faculty, University of Trunojoyo Madura. Research of Assessment of resistance to Downy Mildew used a randomized block design with 18 treatments (P1, P2, F1, F2, BC1P1 and BC1P2 in three sets of crosses, namely LGL x Mdr-3, T12 x Mdr-1 and E02 x Mdr-2) and three replications so there were 54 experimental units. Identification of polymorphic RAPD markers for endurance to downy mildew through Bulk Segregant Analysis (BSA) was done by amplifying the DNA in the resistant pool and susceptible pool. The random primers used were 120 primers from 6 operon groups, namely OPA, OPB, OPC, OPD, OPF and OPG. The results showed that the inheritance pattern of maize genetic resistance to downy mildew followed a segregation pattern of 3:1 with a degree of dominance between -1 and 0, and was controlled by incomplete partially negative dominant gene. OPC-07 was a marker that was linkage close to the resistance to downy mildew with a genetic distance of 1.9 cM.
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