Dissertations / Theses on the topic 'Generative classifiers'
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Xue, Jinghao. "Aspects of generative and discriminative classifiers." Thesis, Connect to e-thesis, 2008. http://theses.gla.ac.uk/272/.
Full textPh.D. thesis submitted to the Department of Statistics, Faculty of Information and Mathematical Sciences, University of Glasgow, 2008. Includes bibliographical references. Print version also available.
ROGER-YUN, Soyoung. "Les expressions nominales à classificateurs et les propositions à cas multiples du coréen : recherches sur leur syntaxe interne et mise en évidence de quelques convergences structurales." Phd thesis, Université de la Sorbonne nouvelle - Paris III, 2002. http://tel.archives-ouvertes.fr/tel-00002834.
Full textMcClintick, Kyle W. "Training Data Generation Framework For Machine-Learning Based Classifiers." Digital WPI, 2018. https://digitalcommons.wpi.edu/etd-theses/1276.
Full textGuo, Hong Yu. "Multiple classifier combination through ensembles and data generation." Thesis, University of Ottawa (Canada), 2004. http://hdl.handle.net/10393/26648.
Full textKang, Dae-Ki. "Abstraction, aggregation and recursion for generating accurate and simple classifiers." [Ames, Iowa : Iowa State University], 2006.
Find full textKimura, Takayuki. "RNA-protein structure classifiers incorporated into second-generation statistical potentials." Thesis, San Jose State University, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10241445.
Full textComputational modeling of RNA-protein interactions remains an important endeavor. However, exclusively all-atom approaches that model RNA-protein interactions via molecular dynamics are often problematic in their application. One possible alternative is the implementation of hierarchical approaches, first efficiently exploring configurational space with a coarse-grained representation of the RNA and protein. Subsequently, the lowest energy set of such coarse-grained models can be used as scaffolds for all-atom placements, a standard method in modeling protein 3D-structure. However, the coarse-grained modeling likely will require improved ribonucleotide-amino acid potentials as applied to coarse-grained structures. As a first step we downloaded 1,345 PDB files and clustered them with PISCES to obtain a non-redundant complex data set. The contacts were divided into nine types with DSSR according to the 3D structure of RNA and then 9 sets of potentials were calculated. The potentials were applied to score fifty thousand poses generated by FTDock for twenty-one standard RNA-protein complexes. The results compare favorably to existing RNA-protein potentials. Future research will optimize and test such combined potentials.
Alani, Shayma. "Design of intelligent ensembled classifiers combination methods." Thesis, Brunel University, 2015. http://bura.brunel.ac.uk/handle/2438/12793.
Full textDING, ZEJIN. "Diversified Ensemble Classifiers for Highly Imbalanced Data Learning and their Application in Bioinformatics." Digital Archive @ GSU, 2011. http://digitalarchive.gsu.edu/cs_diss/60.
Full textSvénsen, Johan F. M. "GTM: the generative topographic mapping." Thesis, Aston University, 1998. http://publications.aston.ac.uk/1245/.
Full textPyon, Yoon Soo. "Variant Detection Using Next Generation Sequencing Data." Case Western Reserve University School of Graduate Studies / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=case1347053645.
Full textBrody, Samuel. "Closing the gap in WSD : supervised results with unsupervised methods." Thesis, University of Edinburgh, 2009. http://hdl.handle.net/1842/3981.
Full textLee, Chang Hee. "Synaesthesia materialisation : approaches to applying synaesthesia as a provocation for generating creative ideas within the context of design." Thesis, Royal College of Art, 2019. http://researchonline.rca.ac.uk/3756/.
Full textLibosvár, Jakub. "Generování modelů domů pro Open Street Mapy." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2013. http://www.nusl.cz/ntk/nusl-236184.
Full textAlam, Sameer Information Technology & Electrical Engineering Australian Defence Force Academy UNSW. "Evolving complexity towards risk : a massive scenario generation approach for evaluating advanced air traffic management concepts." Awarded by:University of New South Wales - Australian Defence Force Academy, 2008. http://handle.unsw.edu.au/1959.4/38966.
Full textMugtussids, Iossif B. "Flight Data Processing Techniques to Identify Unusual Events." Diss., Virginia Tech, 2000. http://hdl.handle.net/10919/28095.
Full textPh. D.
Thanjavur, Bhaaskar Kiran Vishal. "Automatic generation of hardware Tree Classifiers." Thesis, 2017. https://hdl.handle.net/2144/23688.
Full textCorreia, João Nuno Gonçalves Costa Cavaleiro. "Evolutionary Computation for Classifier Assessment and Improvement." Doctoral thesis, 2018. http://hdl.handle.net/10316/81287.
Full textTypical Machine Learning (ML) approaches rely on a dataset and a model to solve problems. For most problems, optimisation of ML approaches is crucial to attain competitive performances. Most of the effort goes towards optimising the model by exploring new algorithms and tuning the parameters. Nevertheless, the dataset is also a key part for ML performance. Gathering, constructing and optimising a representative dataset is a hard task and a time-consuming endeavour, with no well-established guidelines to follow. In this thesis, we attest the use of Evolutionary Computation (EC) to assess and improve classifiers via synthesis of new instances. An analysis of the state of the art on dataset construction is performed. The quality of the dataset is tied to the availability of data, which in most cases is hard to control. A throughout analysis is made about Instance Selection and Instance Generation, which sheds light on relevant points for the development of our framework. The Evolutionary Framework for Classifier Assessment and Improvement (EFECTIVE) is introduced and explored. The key parts of the framework are identified: the Classifier System (CS) module, which holds the ML model that is going to be assessed and improved; the EC module responsible for generating the new instances using the CS module for fitness assignment; and the Supervisor, a module responsible for managing the instances that are generated. The approach comes together in an iterative process of automatic assessment and improvement of classifiers. In a first phase, EFECTIVE is tested as a generator, creating instances of a particular class. Without loss of generality, we apply the framework in the domain of image generation. The problem that motivated the approach is presented first: frontal face generation. In this case, the framework relies on the combination of an EC engine and a CS module, i. e., a frontal face detector, to generate images of frontal faces. The results were revealing in two different ways. On the one hand, the approach was able to generate images that from a subjective standpoint resemble faces and are classified as such by the classifier. On the other hand, most of the images did not resemble faces, although they were classified as such by the classifier module. Based on the results, we extended the approach to generate other types of object, attaining similar results. We also combined several classifiers to study the evolution of ambiguous images, i. e. images that induce multistable perception. Overall, the results suggest that the framework is viable as a generator of instances and also that these instances are often misclassified by the CS module. Building on these results, in a second phase, a study of EFECTIVE for improving the performance of classifiers is performed. The core idea is to use the evolved instances that are generated by the EC engine to augment the training dataset. In this phase, the framework uses the Supervisor module to select and filter the instances that will be added to the dataset. The retraining of the classifier with these instances completes an iteration of the framework. We tested this pipeline in a face detection problem evolving instances to: (i) expand the negative dataset; (ii) expand the positive dataset; and (iii) expand both datasets in the same iteration. Overall, the results show that: expanding the negative dataset, by adding misclassified instances, reduces the number of false alarms; expanding the positive dataset increases the number of hits; expanding positive and negative datasets allows the simultaneous reduction of false alarms and increase of hits. After demonstrating the adequacy of EFECTIVE in face detection, we tested the framework in a Computational Creativity context to create an image generation system that promotes style change, obtaining results that further demonstrate the potential of the framework.
As abordagens típicas de Aprendizagem de Máquina (AM) dependem de um conjunto de instâncias e de um modelo para resolver problemas. Para a maioria dos problemas, a otimização das abordagens AM é crucial para obter desempenhos competitivos. A maior parte do esforço vai no sentido de otimizar o modelo através da exploração de novos algoritmos e do ajuste de parâmetros. No entanto, o conjunto de instâncias é também parte fundamental no desempenho de abordagens de AM. Reunir, construir e otimizar um conjunto de instâncias representativo é uma tarefa difícil e morosa, sem diretrizes bem estabelecidas. Nesta tese, atestamos o uso de Computação Evolucionária (CE) para avaliação e aperfeiçoamento de classificadores através da síntese de novas instâncias. Efetuou-se uma análise do estado da arte sobre construção de conjunto de instâncias. A qualidade do conjunto de instâncias está ligada à disponibilidade de dados que, na maioria dos casos, é difícil de controlar. Uma análise completa é feita sobre a seleção e geração de instâncias, o que esclarece pontos relevantes para o desenvolvimento do nosso sistema. O EFECTIVE (Sistema Evolucionário para a Avaliação e Melhoria de Classificadores) é introduzido e explorado. Os componentes principais do sistema são: o módulo sistema de classificação SC, que contém o modelo de AM que será avaliado e melhorado; por o módulo de CE responsável por gerar as novas instâncias usando o módulo SC para atribuição da aptidão; e o Supervisor, um módulo responsável por gerir as instâncias geradas. A abordagem consiste num processo iterativo de avaliação automática e aprimoramento de classificadores. Numa primeira fase, o EFECTIVE é testado como gerador, criando instâncias de uma classe em particular. Sem perda de generalidade, aplicamos o sistema no domínio da geração de imagens. O problema que motivou a abordagem é apresentado em primeiro lugar: geração de imagens de faces frontais. Neste caso, o sistema depende da combinação de um motor de CE e de um módulo SC, i. e., um detector de faces frontais, para gerar imagens de faces frontais. Os resultados foram reveladores de duas maneiras distintas. Por um lado, a abordagem foi capaz de gerar imagens que, de um ponto de vista subjectivo, se assemelham a faces e são classificadas como tal pelo classificador. Por outro lado, a maior parte das imagens não se parecem com faces, muito embora tenham sido classificadas como tal por parte do classificador. Com base nos resultados estendemos a abordagem para gerar outro tipo de objectos, obtendo resultados similares. Também se combinou vários classificadores num estudo sobre evolução de imagens ambíguas, i. e., imagens que induzem perceção multiestável. De um modo geral, os resultados sugerem que o sistema é viável como um gerador de instâncias e que essas instâncias são muitas vezes mal classificadas pelo SC. Com base nos resultados obtidos, numa segunda fase, efectuámos o estudo sobre o EFECTIVE para aprimoramento da performance de classificadores. A ideia central é utilizar as instâncias geradas pelo motor de CE para aumentar o conjunto de dados de treino do classificador. Nesta fase, o sistema usa o módulo Supervisor para selecionar e filtrar as instâncias que serão adicionadas ao conjunto de treino. O re-treinar do classificador com essas instâncias completa uma iteração do sistema. Testou-se este processo num problema de deteção de faces, evoluindo instâncias para: (i) expandir o conjunto dos negativos; (ii) expandir o conjunto dos positivos; e (iii) expandir ambos os conjuntos na mesma iteração. De um modo geral, os resultados mostram que: expandindo o conjunto dos negativos, adicionando instâncias mal classificadas, reduz o número de falsos alarmes; expandindo o conjunto dos positivos aumenta o número de caras bem detetadas; expandindo o conjunto dos positivos e dos negativos ao mesmo tempo resulta na redução de falsos alarmes e no aumento de caras bem detetadas. Após demonstrar a adequação do EFECTIVE na deteção de faces, testamos o sistema num contexto de Criatividade Computacional para criar um sistema de geração de imagens que promove mudança de estilo, obtendo resultados que demonstram o potencial do sistema.
Cumbo, Chiara, Pasquale Rullo, and Annamaria Canino. "A techinique for automatic generation of rule-based text classifiers exploiting negative information." Thesis, 2014. http://hdl.handle.net/10955/499.
Full textWang, Chi-Cheng, and 王啟誠. "A generation of fuzzy classifier directly from numerical data based on hypercube region." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/84843856344367061011.
Full text國立臺灣科技大學
電機工程系
90
This thesis proposed a new method for extracting fuzzy rules directly from numerical data for pattern classification. First, we represent the existence region of data for a class by activation hypercube and define the overlapping region of each activation hypercube by inhibition hypercube to inhibit the existence of data for that class. Then, we generate dynamic cluster for the data that exist in the inhibition hypercube. Our fuzzy classifier composed of fuzzy rules that are described by these hypercubes. Finally, some examples are given to demonstrate the performance and the validity of this algorithm.
Ding, Zejin. "Diversified Ensemble Classifiers for Highly Imbalanced Data Learning and their Application in Bioinformatics." 2011. http://scholarworks.gsu.edu/cs_diss/60.
Full textSilva, Gabriel Augusto Santos. "Data augmentation and deep classification with generative adversarial networks." Master's thesis, 2021. http://hdl.handle.net/10773/32283.
Full textAprendizagem automática tem visto bastantes melhorias em anos recentes. Um tipo de modelo que tem evoluído bastante são as Generative Adversarial Networks (GANs). Estes modelos têm a capacidade de criar dados falsos que se assemelham aos dados em que foram treinados. O interesse por estes modelos tem vindo a crescer desde a sua criação, em 2014. Tem-se vindo a provar que a possibilidade de criar dados falsos pode ser bastante útil, especialmente, em áreas com pouca abundância de dados, como é o caso da imagem médica. As GANs têm sido usadas, com bastante sucesso, nesse tipo de áreas para aumentar o tamanho dos datasets existentes de modo a melhorar a qualidade dos classificadores usados. Esta dissertação faz um estudo com um tipo específico de GAN, a Auxiliary Classification GAN (AC-GAN), para perceber se existem novas formas para as GANs melhorarem a qualidade de classificadores. Para isso, uma experiência de três partes foi desenhada, sendo cada parte designada como um Cenário. No Cenário 1 um classificador isolado foi treinado, no Cenário 2 foi treinado um classificador igual após uma GAN ter sido usada para fazer aumento de dados e, finalmente, no Cenário 3 usou-se uma AC-GAN em vez de um classificador. Foram considerados dois problemas distintos. O primeiro foi o CIFAR-10, que é um problema bastante conhecido e bem estruturado, usado com muita frequência em problemas relacionados com GANs. O segundo problema usado foi um de lesões de pele. Isto serviu dois propósitos: aumentar significativamente a dificuldade do problema em mão e aproximar o trabalho feito aqui com um dos maiores usos práticos das GANs, que tem sido o uso de GANs para fazer o aumento de datasets em problemas de imagem médica. Os modelos desenvolvidos foram baseados na AC-GAN original e na BigGAN, que, quando foi apresentada, era a melhor GAN conhecida e era capaz de produzir imagens de alta qualidade com resoluções de até 512x512. Adaptar a BigGAN para uma AC-GAN resultou na melhor AC-GAN conhecida treinada no dataset CIFAR-10. O estudo feito nesta dissertação pode servir como uma base sólida para que mais estudos sejam feitos neste âmbito, visto que os resultados obtidos aqui sugerem firmemente que o uso de AC-GANs pode ser uma forma efetiva de atingir classificadores melhores.
Mestrado em Engenharia de Computadores e Telemática
Dattagupta, Samrat Jayanta. "A performance comparison of oversampling methods for data generation in imbalanced learning tasks." Master's thesis, 2018. http://hdl.handle.net/10362/31307.
Full textClass Imbalance problem is one of the most fundamental challenges faced by the machine learning community. The imbalance refers to number of instances in the class of interest being relatively low, as compared to the rest of the data. Sampling is a common technique for dealing with this problem. A number of over - sampling approaches have been applied in an attempt to balance the classes. This study provides an overview of the issue of class imbalance and attempts to examine some common oversampling approaches for dealing with this problem. In order to illustrate the differences, an experiment is conducted using multiple simulated data sets for comparing the performance of these oversampling methods on different classifiers based on various evaluation criteria. In addition, the effect of different parameters, such as number of features and imbalance ratio, on the classifier performance is also evaluated.
(11167785), Nicolae Christophe Iovanac. "GENERATIVE, PREDICTIVE, AND REACTIVE MODELS FOR DATA SCARCE PROBLEMS IN CHEMICAL ENGINEERING." Thesis, 2021.
Find full text(6997520), Bo Zhang. "A DESIGN PARADIGM FOR DC GENERATION SYSTEM." Thesis, 2020.
Find full text(6890684), Nicole E. Eikmeier. "Spectral Properties and Generation of Realistic Networks." Thesis, 2019.
Find full text(7027607), Zhengtian Song. "Second Harmonic Generation Microscopy and Raman Microscopy of Pharmaceutical Materials." Thesis, 2019.
Find full textSecond harmonic generation (SHG) microscopy and Raman microscopy were used for qualitative and quantitative analysis of pharmaceutical materials. Prototype instruments and algorithms for sampling strategies and data analyses were developed to achieve pharmaceutical materials analysis with low limits of detection and short measurement times
Manufacturing an amorphous solid dispersion (ASD), in which an amorphous active pharmaceutical ingredient (API) within polymer matrix, is an effective approach to improve the solubility and bioavailability of a drug. However, since ASDs are generally metastable materials, they can often transform to produce crystalline API with higher thermodynamic stability. Analytical methods with low limits of detection for crystalline APIs were used to assess the stability of ASDs. With high selectivity to noncentrosymmetric crystals, SHG microscopy was demonstrated as an analytical tool, which exhibited a limit of detection of 10 ppm for ritonavir Form II crystals. SHG microscopy was employed for accelerated stability testing of ASDs, which provided a four-decade dynamic range of crystallinity for kinetic modeling. An established model was validated by investigating nucleation and crystal growth based on SHG images. To achieve in situ accelerated stability testing, controlled environment for in situstability testing (CEiST) was designed and built to provide elevated temperature and humidity, which is compatible with a commercial SHG microscope based on our research prototype. The combination of CEiST and SHG microscopy enabled assessment of individual crystal growth rates by single-particle tracking and nucleation rates for individual fields of view with low Poisson noise. In addition, SHG microscopy coupled with CEiST enabled the study of heterogeneity of crystallization kinetics within pharmaceutical materials.
Polymorphism of APIs plays an important role in drug formulation development. Different polymorphs of identical APIs may exhibit different physiochemical properties, e.g., solubility, stability, and bioavailability, due to their crystal structures. Moreover, polymorph transitions may take place during the manufacturing process and storage. Therefore, analytical methods with high speed for polymorph characterization, which can provide real-time feedback for the polymorphic transition, have broad applications in pharmaceutical materials characterization. Raman spectroscopy is able to determine the API polymorphism, but is hampered by the long measurement times. In this study, two analytical methods with high speed were developed to characterize API polymorphs. One is SHGmicroscopy-guided Raman spectroscopy, which achieved the speed of 10 ms/particle for clopidogrel bisulfate. Initial classification of two different polymorphs was based on SHG images, followed acquisition of Raman spectroscopyat the selected positions to determine the API crystal form. Another approach is implementing of dynamic sampling into confocal Raman microscopy to accelerate Raman image acquisition for 6-folds. Instead of raster scanning, dynamic sampling algorithm enabled acquiring Raman spectra at the most informative locations. The reconstructed Raman image of pharmaceutical materials has <0.5% loss of image quality with 15.8% sampling rate.
(8772923), Chinyi Chen. "Quantum phenomena for next generation computing." Thesis, 2020.
Find full text(11142147), Zachary Craig Schreiber. "Investigation of Transparent Photovoltaic Vehicle Integration." Thesis, 2021.
Find full text(6997700), Wooram Kang. "HYDROGEN GENERATION FROM HYDROUS HYDRAZINE DECOMPOSITION OVER SOLUTION COMBUSTION SYNTHESIZED NICKEL-BASED CATALYSTS." Thesis, 2019.
Find full text(9780926), Muhammad Bhuiya. "An experimental study of 2nd generation biodiesel as an alternative fuel for diesel engine." Thesis, 2017. https://figshare.com/articles/thesis/An_experimental_study_of_2nd_generation_biodiesel_as_an_alternative_fuel_for_diesel_engine/13449476.
Full text(6887678), Oscar E. Sandoval. "Electro-Optic Phase Modulation, Frequency Comb Generation, Nonlinear Spectral Broadening, and Applications." Thesis, 2019.
Find full textElectro-optic phase modulation can be used to generate high repetition rate optical frequency combs. The optical frequency comb (OFC) has garnered much attention upon its inception, acting as a crucial component in applications ranging from metrology and spectroscopy, to optical communications. Electro-optic frequency combs (EO combs) can be generated by concatenating an intensity modulator and phase modulator together. The first part of this work focuses on broadening the modest bandwidth inherent to the EO combs. This is achieved by propagation in a nonlinear medium, specifically propagation in a nonlinear optical loop mirror (NOLM). This allows for broadening the EO frequency comb spectrum to a bandwidth of 40 nm with a spectral power variation of < 10 dB. This spectrally broadened EO comb is then used in dual comb interferometry measurements to characterize the single soliton generated in an anomalous dispersion silicone-nitride microresonator. This measurement allows for rapid characterization with low average power. Finally, electro-optic phase modulation is used in a technique to prove frequency-bin entanglement. A quantum network based on optical fiber will require the ability to perform phase modulation independent of photon polarization due to propagation in optical fiber scrambling the polarization of input light. Commercially available phase modulators are inherently dependent on the polarization state of input light making them unsuited to be used in such a depolarized environment. This limitation is overcome by implementing a polarization diversity scheme to measure frequency-bin entanglement for arbitrary orientations of co- and cross- polarized frequency-bin entangled photon pairs.
(8788166), Scott R. Griffin. "Optical Techniques for Analysis of Pharmaceutical Formulations." Thesis, 2020.
Find full textThe symmetry requirements of both second harmonic generation (SHG) and triboluminescence (TL) provide outstanding selectivity to noncentrosymmetric crystals, leading to high signal to noise measurements of crystal growth and nucleation of active pharmaceutical ingredients (API) within amorphous solid dispersions (ASD) during accelerated stability testing. ASD formulations are becoming increasingly popular in the pharmaceutical industry due to their ability to address challenges associated with APIs that suffer from poor dissolution kinetics and low bioavailability as a result of low aqueous solubility. ASDs kinetically trap APIs into an amorphous state by dispersing the API molecules within a polymer matrix. The amorphous state of the API leads to an increase in apparent solubility, faster dissolution kinetics, and an increase in bioavailability. Both SHG and TL are used to quantitatively and qualitatively detect the crystal growth and nucleation within ASD formulations at the parts per million (ppm) regime. TL is the emission of light upon mechanical disruption of a piezoelectrically active crystal. Instrumentation was developed to rapidly determine the qualitative presence of crystals within nominally amorphous pharmaceutical materials in both powders and slurries. SHG was coupled with a controlled environment for in situ stability testing (CEiST) to enable in situ accelerated stability testing of ASDs. Single particle tracking enabled by the CEiST measurements provided insights into crystal growth rate distributions present due to local differences within the material. Accelerated stability testing monitored by in situ measurements increased the signal to noise in recovered nucleation and crystal growth rates by suppressing the Poisson noise normally present within conventional accelerated stability tests. The disparities between crystal growth and nucleation kinetics on the surface versus within bulk material were also investigated by single particle tracking and in situ measurements. Crystals were found to grow faster in the bulk compared to single crystals growing on the surface while total crystallinity was found to be higher on the surface due to radial growth habits of crystals on the surface compared to columnar growth within the bulk. To increase the throughput of the in situ measurements, a temperature and relative humidity array (TRHA) was developed. The TRHA utilizes a temperature gradient and many individual liquid wells to enable the use of a multitude of different conditions at the same time which can reduce time required to inform formulations design of stability information.
(9167615), Orthi Sikder. "Influence of Size and Interface Effects of Silicon Nanowire and Nanosheet for Ultra-Scaled Next Generation Transistors." Thesis, 2020.
Find full text(8966861), Dina Verdin. "Enacting Agency: Understanding How First-Generation College Students’ Personal Agency Supports Disciplinary Role Identities and Engineering Agency Beliefs." Thesis, 2020.
Find full textThis dissertation is a three study format. In this dissertation, I used an explanatory sequential mixed method design. Study 1 develops a measurement scale to capture first-generation college students’ agency using the constructs of intentionality, forethought, self-reactiveness, and self-reflectiveness. Study 2 used structural equation modeling to establish a relationship between personal agency, disciplinary role identities, and students’ desire to enact engineering agency. Study 3 was a narrative analysis of how Kitatoi, a Latina, first-generation college student, authored her identity as an engineer. Data for study 1 and 2 came from a survey administered in the Fall of 2017 of 3,711 first-year engineering students across 32 ABET universities.
(9127556), Hilary M. Florian. "IMPROVING THE PROTEIN PIPELINE THROUGH NONLINEAR OPTICAL METHODS." Thesis, 2020.
Find full textUnderstanding the function and structure of a protein is crucial for informing on rational drug design and for developing successful drug candidates. However, this understanding is often limited by the protein pipeline, i.e. the necessary steps to go from developing protein constructs to generating high-resolution structures of macromolecules. Because each step of the protein pipeline requires successful completion of the prior step, bottlenecks are often created and therefore this process can take up to several years to complete. Addressing current limitations in the protein pipeline can help to reduce the time required to successfully solve the structure of a protein.
The field of nonlinear optical (NLO) microscopy provides a potential solution to many issues surrounding the detection and characterization of protein crystals. Techniques such as second harmonic generation (SHG) and two-photon excited UV fluorescence (TPE-UVF) have already been shown to be effective methods for the detection of proteins with high selectivity and sensitivity. Efforts to improve high throughput capabilities of SHG microscopy for crystallization trials resulted in development of a custom microretarder array (μRA) for depth of field (DoF) extension, therefore eliminating the need for z-scanning and reducing the overall data acquisition time. Further work was done with a commercially available μRA to allow for polarization dependent TPE-UVF. By placing the μRA in the rear conjugate plane of the beam path, the patterned polarization was mapped onto the field of view and polarization information was extracted from images by Fourier analysis to aid in discrimination between crystalline and aggregate protein.
Additionally, improvements to X-ray diffraction (XRD), the current gold standard for macromolecular structure elucidation, can result in improved resolution for structure determination. X-ray induced damage to protein crystals is one of the greatest sources of loss in resolution. Previous work has been done to implement a multimodal nonlinear optical (NLO) microscope into the beamline at Argonne National Lab. This instrument aids in crystal positioning for XRD experiments by eliminating the need for X-ray rastering and reduces the overall X-ray dosage to the sample. Modifications to the system to continuously improve the capabilities of the instrument were done, focusing on redesign of the beam path to allow for epi detection of TPE-UVF and building a custom objective for improved throughput of 1064 nm light. Furthermore, a computational method using non-negative matrix factorization (NMF) was employed for isolation of unperturbed diffraction peaks and provided insight into the mechanism by which X-ray damage occurs. This work has the potential to improve the resolution of diffraction data and can be applied to other techniques where X-ray damage is of concern, such as electron microscopy.
(11022585), Bhavya Rathna Kota. "Investigation of GenerationZs' perception of Green Homes and Green Home Features." Thesis, 2021.
Find full text(6331859), Changqin Ding. "Polarization-enabled Multidimensional Optical Microscopy." Thesis, 2019.
Find full text(6397766), Shaobo Fang. "SINGLE VIEW RECONSTRUCTION FOR FOOD PORTION ESTIMATION." Thesis, 2019.
Find full text3D scene reconstruction based on single-view images is an ill-posed problem since most 3D information has been lost during the projection process from the 3D world coordinates to the 2D pixel coordinates. To estimate the portion of an object from a single-view requires either the use of priori information such as the geometric shape of the object, or training based techniques that learn from existing portion sizes distribution. In this thesis, we present a single-view based technique for food portion size estimation.
Dietary assessment, the process of determining what someone eats during the course of a day, provides valuable insights for mounting intervention programs for prevention of many chronic diseases such as cancer, diabetes and heart diseases. Measuring accurate dietary intake is considered to be an open research problem in the nutrition and health fields. We have developed a mobile dietary assessment system, the Technology Assisted Dietary AssessmentTM (TADATM) system to automatically determine the food types and energy consumed by a user using image analysis techniques.
In this thesis we focus on the use of a single image for food portion size estimation to reduce a user’s burden from having to take multiple images of their meal. We define portion size estimation as the process of determining how much food (or food energy/nutrient) is present in the food image. In addition to estimating food energy/nutrient, food portion estimation could also be estimating food volumes (in cm3) or weights (in grams), as they are directly related to food energy/nutrient. Food portion estimation is a challenging problem as food preparation and consumption process can pose large variations in food shapes and appearances.
As single-view based 3D reconstruction is in general an ill-posed problem, we investigate the use of geometric models such as the shape of a container that can help to partially recover 3D parameters of food items in the scene. We compare the performance of portion estimation technique based on 3D geometric models to techniques using depth maps. We have shown that more accurate estimation can be obtained by using geometric models for objects whose 3D shape are well defined. To further improve the food estimation accuracy we investigate the use of food portions co-occurrence patterns. The food portion co-occurrence patterns can be estimated from food image dataset we collected from dietary studies using the mobile Food RecordTM (mFRTM) system we developed. Co-occurrence patterns is used as prior knowledge to refine portion estimation results. We have been shown that the portion estimation accuracy has been improved when incorporating the co-occurrence patterns as contextual information.
In addition to food portion estimation techniques that are based on geometric models, we also investigate the use deep learning approach. In the geometric model based approach, we have focused on estimation food volumes. However, food volumes are not the final results that directly show food energy/nutrient consumed. Therefore, instead of developing food portion estimation techniques that lead to an intermediate results (food volumes), we present a food portion estimation method to directly estimate food energy (kilocalories) from food images using Generative Adversarial Networks (GANs). We introduce the concept of an “energy distribution” for each food image. To train the GAN, we design a food image dataset based on ground truth food labels and segmentation masks for each food image as well as energy information associated with the food image. Our goal is to learn the mapping of the food image to the food energy. We then estimate food energy based on the estimated energy distribution image. Based on the estimated energy distribution image, we use a Convolutional Neural Networks (CNN) to estimate the numeric values of food energy presented in the eating scene.
(9735716), Phyllis Antwiwaa Agyapong. "Examining the Relationship Between Parental Sex Education, Religiosity And Sex Positivity In First- And Second-Generation African Immigrants." Thesis, 2020.
Find full textThis quantitative study examined the relationship between parental comprehensive sexual and reproductive health communication (SRH), religiosity and sex positivity in first- and second-generation African immigrants. Comprehensive SRH communication was measured by frequency through the Sexual Communication Scale (SCS), religiosity was measured through the Faith Activities in the Home Scale (FAITHS) and sex positivity was measured through the Sex Positivity Scale (SPS). It was hypothesized that there would be a negative relationship between religiosity and sex positivity and a positive relationship between religiosity and sex positivity in first-and second-generation African immigrants. Results indicated that higher levels of religiosity in the participant’s upbringing was significantly associated with higher sex positivity. Additional findings revealed higher instances of SRH communication correlated with higher sex positivity in men and lower sex positivity in women. This study aimed to set a foundation for future studies on first- and second-generation African immigrants as it relates to sexual health.
(9780230), Sharmina Begum. "Assessment of alternative waste technologies for energy recovery from solid waste in Australia." Thesis, 2016. https://figshare.com/articles/thesis/Assessment_of_alternative_waste_technologies_for_energy_recovery_from_solid_waste_in_Australia/13436876.
Full text(9838253), Roshani Subedi. "Assessing the viability of growing Agave Tequilana for biofuel production in Australia." Thesis, 2013. https://figshare.com/articles/thesis/Assessing_the_viability_of_growing_Agave_Tequilana_for_biofuel_production_in_Australia/20459547.
Full textGovernments around the world have been introducing policies to support the use of biofuels since the 1990s due to its positive influence in climate change mitigation, air quality, fuel supply security and poverty reduction through rural and regional iindustry growth. In Australia, liquid fuel is in high demand and this demand is increasing every year. To meet the current fuel demand and to address climate change impacts, it is important for Australia to invest in green and clean energy. Biofuels are one of the options for clean and green energy that could help to reduce the demand for fossil fuels. Not only developed countries but also developing countries are interested in reducing dependence on imported fossil fuel and promoting economic development, poverty reductions and improving access to commercial energy through biofuel policies. However, the major challenge for the biofuel industry is to find the right feedstock that does not compete with human feedstock and can grow in marginal land. One of such feedstock that is studied in this research is Agave tequilana.
Overcoming many of the constraints to establish Agave tequilana as a potential feedstock in Australia requires an understanding of the complex technical, economical and systemic challenges associated with farming, processing and extracting ethanol. The aim of this research is to study the viability of growing Agave tequilana as a potential biofuel feedstock in Australia. The study also explores and highlights the economics of growing this crop, with the idea of comparing the costs and benefits of growing Agave tequilana with that of sugarcane. Agave tequilana has been selected for this study because of the existence of a trial site at Ayr, Queensland and because of a similar climate and rainfall pattern to that of the western central highlands of Mexico where Agave is traditionally grown for the production of tequila. In this study, the viability of growing Agave tequilana for producing ethanol in Ayr, Queensland has been assessed using a case study approach and financial cost and Green House Gas (GHG) saving have been estimated using life cycle cost analysis. Likewise, Agave tequilana and sugarcane agronomic practices have been compared and ibofuel policies have been highlighted using secondary sources to support the establishment of non-food crops such as Agave tequilana in Australia and elsewhere.
Ayr, Queensland is predominantly a sugarcane growing area where sugarcane farmers occupy 88% of the total agricultural land available. The remaining 12% has been set aside for other crops and cattle grazing or alternatively, some land may remain unused. In this study, farmers expressed that there is very limited land in Ayr available for Agave tequilana to be commercially viable until the sugarcane growing land or cattle grazing land is converted into Agave fields. However, it appears that both farmers and stakeholders are ready to accept Agave tequilana as a potential biofuel crop, if it is to be established on marginal lands in the sugarcane belt of Queensland, rather than in the Burdekin region which is predominately a sugarcane growing area.
The study also found that only 33% respondents were acquainted with this crop, and that a smaller group were aware of the potential of the crop to produce biofuel. Farmers indicated they would wait until the first trial outcomes are finalised and more research and development is undertaken on this crop before deciding to invest. Since this crop takes at least five years to provide a financial return compared to existing crops in the region, most of the respondents expect higher returns of 20-25% at the end of harvesting time and would prefer interim payment. Farmers may also require initial assistance from the government such as subsidised farm machinery, subsidised fuel and interest free loans before deciding to invest. Life cycle stages of Agave tequilana have been derived taking sugarcane as a base crop. At the first trial site, more than 65% of the cost of farming Agave tequilana in Australia occurred in the first year of plantation, and allowed the conclusion that existing tools and machineries are able to be modified and used in farming Agave tequilana in Australia. The tequila
industry provides a model for biofuel production from Agave tequilana. In Australia, the cost of producing ethanol from Agave tequilana is estimated to be around A$0.52 per litre, excluding government subsidies. The total cost of constructing ethanol pl nt capacity of 90
ML/Year in Australia at present is estimated at A$113.5 million.
The level of support provided to the biofuel industry by the Australian government is relatively less significant compared to other advanced countries such as USA and EU. However, the support provided by both the federal and state level programs has provided significant amounts of support to the biofuel industry in Australia. In future, if Agave tequilana is to be selected as a potential non-food crop biofuel feedstock, the government and the private sector need to explore the financial opportunities in marginal and semi marginal regions of Australia for supplementing the viability of producing ethanol with new technology. It is also necessary to explore the business case to modify the existing sugar processing mills to produce ethanol from Agave tequilana from its juice and bagasse.
(5929979), Yun-Jou Lin. "Point Cloud-Based Analysis and Modelling of Urban Environments and Transportation Corridors." Thesis, 2019.
Find full text(10725756), Duncan N. Houpt. "Synthesis of High-Performance Supercapacitor Electrodes using a CNT-ZIF-8-MoS2 Framework." Thesis, 2021.
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