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Journal articles on the topic 'Gene PARL'

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Published: 10 March 2023

Last updated: 11 March 2023

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1

Merhi, Rawan, Michael Kalyn, Amanda Zhu-Pawlowsky, and Marc Ekker. "Loss of parla Function Results in Inactivity, Olfactory Impairment, and Dopamine Neuron Loss in Zebrafish." Biomedicines 9, no. 2 (February 18, 2021): 205. http://dx.doi.org/10.3390/biomedicines9020205.

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The presenilin-associated rhomboid-like (PARL) gene was found to contribute to mitochondrial morphology and function and was linked to familial Parkinson’s disease (PD). The PARL gene product is a mitochondrial intramembrane cleaving protease that acts on a number of mitochondrial proteins involved in mitochondrial morphology, apoptosis, and mitophagy. To date, functional and genetic studies of PARL have been mainly performed in mammals. However, little is known about PARL function and its role in dopaminergic (DA) neuron development in vertebrates. The zebrafish genome comprises two PARL paralogs: parla and parlb. Here, we established a loss-of-function mutation in parla via CRISPR/Cas9-mediated mutagenesis. We examined DA neuron numbers in the adult brain and expression of genes associated with DA neuron function in larvae and adults. We show that loss of parla function results in loss of DA neurons, mainly in the olfactory bulb. Changes in the levels of tyrosine hydroxylase transcripts supported this neuronal loss. Expression of fis1, a gene involved in mitochondrial fission, was increased in parla mutants. Finally, we showed that loss of parla function translates into impaired olfaction and altered locomotion parameters. These results suggest a role for parla in the development and/or maintenance of DA neuron function in zebrafish.

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Liu, J. F., S. L. Zhang, H. L. Tang, L. Z. Wu, L. J. Dong, L. D. Liu, and W. L. Che. "Overexpression of an Aeluropus littoralis Parl. potassium transporter gene, AlHAK1, in cotton enhances potassium uptake and salt tolerance." Euphytica 203, no. 1 (November 26, 2014): 197–209. http://dx.doi.org/10.1007/s10681-014-1310-2.

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3

Istikharah, Rochmy, Aung Win Tun, Supannee Kaewsutthi, Pratibha Aryal, Bussaraporn Kunhapan, Wanphen Katanyoo, Wanicha Chuenkongkaew, and Patcharee Lertrit. "Identification of the variants in PARL, the nuclear modifier gene, responsible for the expression of LHON patients in Thailand." Experimental Eye Research 116 (November 2013): 55–57. http://dx.doi.org/10.1016/j.exer.2013.08.007.

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4

Zhang, A.-Mei, Xiaoyun Jia, Qingjiong Zhang, and Yong-Gang Yao. "No association between the SNPs (rs3749446 and rs1402000) in the PARL gene and LHON in Chinese patients with m.11778G>A." Human Genetics 128, no. 4 (August 14, 2010): 465–68. http://dx.doi.org/10.1007/s00439-010-0875-7.

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5

Ko, Kenton, Jeremy Guenther, Nicholas Ostan, and Joshua Powles. "Analysis of the Arabidopsis organellar rhomboid At1g74140 transcript population uncovered splicing patterns different from its close relative At1g74130." F1000Research 8 (November 18, 2019): 1925. http://dx.doi.org/10.12688/f1000research.21219.1.

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Background: Four distinct rhomboid genes appear to function in Arabidopsis plastids, two “active” types from the secretases and presenilin-like associated rhomboid-like (PARL) categories (At1g25290 and At5g25752) and two “inactive” rhomboid forms (At1g74130 and At1g74140). The number of working rhomboids is further increased by alternative splicing, two reported for At1g25290 and three for At1g74130. Since At1g25290 and At1g74130 exist as alternative splice variants, it would be necessary to assess the splicing patterns of the other two plastid rhomboid genes, At5g25752 and At1g74140, before studying the Arabidopsis plastid rhomboid system as a whole. Methods: This study thus specifically focused on an analysis of the At1g74140 transcript population using various RT-PCR strategies. Results: The exon mapping results indicate splicing patterns different from the close relative At1g74130, despite similarity between the exonic sequences. The splicing patterns indicate a high level of sequence “discontinuity” in the At1g74140 transcript population with a significant portion of the discontinuity being generated by two regions of the gene. Conclusion: The overall discontinuous splicing pattern of At1g74140 may be reflective of its mode of involvement in activities like controlling gene expression.

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Powles, Joshua, Katharine Sedivy-Haley, Eric Chapman, and Kenton Ko. "Characterization of three alternative splice variants associated with the Arabidopsis rhomboid protein gene At1g74130." Botany 91, no. 12 (December 2013): 840–49. http://dx.doi.org/10.1139/cjb-2013-0173.

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Rhomboid serine proteases are grouped into three main types — secretases, presenilin-like associated rhomboid-like (PARL) proteases, and “inactive” rhomboid proteins. Although the three rhomboid groups are distinct, the different types are likely to operate within the same cell or compartment, such as observed in the plastids of Arabidopsis. There are four distinct plastid rhomboid genes at play in Arabidopsis plastids, two for active types (At1g25290 and At5g25752) and two for inactive forms (At1g74130 and At1g74140). The number of working plastid rhomboids is further increased by alternative splicing, as reported for At1g25290. To understand how the plastid rhomboid system works, it is necessary to identify all rhomboid forms in play. To this end, this study was designed to examine the alternative splicing activities of At1g74130, one of the two genes encoding proteolytically “inactive” plastid rhomboids. The exon mapping and DNA sequencing results obtained here indicate the presence of three prominent alternative splice variants in the At1g74130 transcript population. The dominant splice variant, L, encodes the full-length protein. The other two splice variants, M and S, produce proteins lacking sections from the carboxyl transmembrane domain region. The splice variants M and S appear to be at levels with functional potential and appear to adjust relative to each other during development and in response to changes in the level of Tic40, a component of the plastid translocon. The splice variant proteins themselves exhibit different characteristics with respect to rhomboid protein–substrate interactions. These differences were observed in bacterial co-expression pull-down assays and in yeast mitochondrial studies. When considered together, the data suggest that the alternative splicing of At1g74130 bears functional significance in Arabidopsis and is likely to be part of a mechanism for diversifying plastid rhomboid function.

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7

Flores, Andrés, Javier López-Upton, Cristobal D. Rullán-Silva, Adriana E. Olthoff, Ricardo Alía, Cuauhtémoc Sáenz-Romero, and José M. Garcia del Barrio. "Priorities for Conservation and Sustainable Use of Forest Genetic Resources in Four Mexican Pines." Forests 10, no. 8 (August 9, 2019): 675. http://dx.doi.org/10.3390/f10080675.

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The strategies for the conservation and sustainable use of forest genetic resources, which are essential for the future adaptation of forest species to changing environments, are also a source of valuable genetic resources for breeding and restoration activities. The first step to define and implement cost-effective strategies is to identify specific priority populations. Mexico, in spite of being characterized by high levels of tree species diversity, mostly lacks a combined strategy for the genetic conservation and use of forest genetic resources. The aims of this work are: (i) to identify areas for gene conservation, and (ii) to propose measures for the conservation and sustainable use of forest genetic resources of four pine species: Pinus greggii Engelm. ex Parl., Pinus oocarpa Schiede ex Schltdl., Pinus patula Schiede ex Schltdl. & Cham. and Pinus pseudostrobus Lindl. To do that, we use the existing information on the distribution, genetic variation and conservation and breeding efforts in Mexico. Overall, 51 areas for establishing genetic conservation units were prioritized and 6 genetic zones for the use of forest genetic resources in breeding and selection of forest reproductive material were identified. The current conservation efforts for the four priority Mexican pines should be improved to satisfy the needs of a national breeding and conservation network.

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8

Powles, Joshua, and Kenton Ko. "Alternative splice variants of rhomboid proteins: In silico analysis of database entries for select model organisms and validation of functional potential." F1000Research 7 (February 1, 2018): 139. http://dx.doi.org/10.12688/f1000research.13383.1.

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Background: Rhomboid serine proteases are present in many species with sequenced genomes, and are often encoded in each species by more than one predicted gene. Based on protein sequence comparisons, rhomboids can be differentiated into groups - secretases, presenilin-like associated rhomboid-like (PARL) proteases, iRhoms, and “inactive” rhomboid proteins. Although these rhomboid groups are distinct, the different types can operate simultaneously. Studies in Arabidopsis showed that the number of rhomboid proteins working simultaneously can be further diversified by alternative splicing. This phenomenon was confirmed for the Arabidopsis plastid rhomboid proteins At1g25290 and At1g74130. Although alternative splicing was determined to be a significant mechanism for diversifying these two Arabidopsis plastid rhomboids, there has yet to be an assessment as to whether this mechanism extends to other rhomboids and to other species. Methods: We thus conducted a multi-year analysis of databases to determine if the alternative splicing mechanism observed for the two Arabidopsis plastid rhomboids was utilized in other species to expand the repertoire of rhomboid proteins. To help verify the in silico findings, select splice variants from different groups were tested for activity using transgenic- and additive-based assays. These assays aimed to uncover evidence that the selected splice variants display capacities to influence processes like antimicrobial sensitivity. Results: The multi-year in silico assessment for six model experimental species (human, mouse, Arabidopsis, Drosophila, nematode, and yeast) revealed robust usage of alternative splicing to diversify rhomboid protein structure across the various motifs or regions, especially in human, mouse and Arabidopsis. Subsequent validation studies uncover evidence that the splice variants selected for testing displayed functionality in the different activity assays. Conclusions: The combined results support the hypothesis that alternative splicing is likely used to diversify and expand rhomboid protein functionality, and this potentially occurred across the various motifs or regions of the protein.

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Powles, Joshua, and Kenton Ko. "Alternative splice variants of rhomboid proteins: Comparative analysis of database entries for select model organisms and validation of functional potential." F1000Research 7 (May 31, 2018): 139. http://dx.doi.org/10.12688/f1000research.13383.2.

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Background: Rhomboid serine proteases are present across many species and are often encoded in each species by more than one predicted gene. Based on protein sequence comparisons, rhomboids can be differentiated into groups - secretases, presenilin-like associated rhomboid-like (PARL) proteases, iRhoms, and “inactive” rhomboid proteins. Although these rhomboid groups are distinct, the different types can operate simultaneously. Studies in Arabidopsis showed that the number of rhomboid proteins working simultaneously can be further diversified by alternative splicing. This phenomenon was confirmed for the Arabidopsis plastid rhomboid proteins At1g25290 and At1g74130. Although alternative splicing was determined to be a significant mechanism for diversifying these two Arabidopsis plastid rhomboids, there has yet to be an assessment as to whether this mechanism extends to other rhomboids and to other species. Methods: We thus conducted a comparative analysis of select databases to determine if the alternative splicing mechanism observed for the two Arabidopsis plastid rhomboids was utilized in other species to expand the repertoire of rhomboid proteins. To help verify the in silico observations, select splice variants from different groups were tested for activity using transgenic- and additive-based assays. These assays aimed to uncover evidence that the selected splice variants display capacities to influence processes like antimicrobial sensitivity. Results: A comparison of database entries of six widely used eukaryotic experimental models (human, mouse, Arabidopsis, Drosophila, nematode, and yeast) revealed robust usage of alternative splicing to diversify rhomboid protein structure across the various motifs or regions, especially in human, mouse and Arabidopsis. Subsequent validation studies uncover evidence that the splice variants selected for testing displayed functionality in the different activity assays. Conclusions: The combined results support the hypothesis that alternative splicing is likely used to diversify and expand rhomboid protein functionality, and this potentially occurred across the various motifs or regions of the protein.

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10

Sawhney, Shikhar, Sandeep Bansal, Madhur Kalyan, Indu Verma, Ramandeep Singh Virk, and Ashok Kumar Gupta. "Analysis of differential expression of protease-activated receptors in patients with allergic fungal rhinosinusitis." Allergy & Rhinology 9 (January 2018): 215265671876419. http://dx.doi.org/10.1177/2152656718764199.

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Background Ever since its characterization in the 1970s, allergic fungal rhinosinusitis (AFRS) has been the subject of much controversy, especially regarding its pathogenesis. In this study, we analyzed the differential expression of genes that encode protease-activated receptors (PAR) in patients with AFRS and patients with chronic rhinosinusitis, and tried to understand the pathogenic basis of this disease. Objective To analyze the differential expression of PAR genes in patients with AFRS and in patients with chronic rhinosinusitis. Methods Mucosa from ethmoid sinuses of 51 patients (tests and controls) was biopsied and evaluated for messenger RNA expression of PAR genes by using reverse transcriptase–polymerase chain reaction. Each of the four PAR genes, i.e., par1, par2, par3 and par4 was amplified, the final gene products were run on 1.8% agarose gel and analyzed by densitometry to calculate differential expression. The significance level was determined as p ≤ 0.05. Results It was observed that the expressions of all four par genes were higher in the test samples compared with the controls, but statistical significance was achieved only for par1 (p=0.004) and par2 (p=0.05). Comparative expression of the four PAR genes was also performed within the test and control groups, and a statistically significant difference was seen between par1 and par2 (p=0.007), par1 and par3 (p=0.029), par1 and par4 (p=0.0001), par2 and par4 (p=0.002), and par3 and par4 (p=0.009) in the test group. In the control group as well, par1, par2, and par3 exhibited a higher expression compared with par4 but the difference was significant between par3 and par4 genes only. Conclusion Patients with AFRS expressed increased levels of PAR genes in their nasal mucosa, and, of the four PAR genes, a higher expression of par1, par2, and par3 was observed in both the groups compared with par4. This information contributes toward our understanding of pathogenesis and possibly treatment of AFRS.

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11

Eickhoff, Hajo. "Auf Leben und Tod. Töten als Attraktion." Paragrana 20, no. 1 (August 2011): 51–63. http://dx.doi.org/10.1524/para.2011.0004.

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ZusammenfassungTöten wird unter der Perspektive der Attraktion betrachtet. Angreifer stellen eine kritische Nähe zu Lebewesen her, überschreiten deren Grenze und trennen von ihnen ab, was sie lebendig sein lässt. Dieses Töten als Trennen von Verbundenem eint die Seinsbereiche Physik, Chemie, Biologie und Kultur. Untersucht werden die verschiedenen Attraktionen, zu töten. Tiere töten, um sich zu ernähren, können aber artgleiche Tiere töten, wenn sie ein Areal oder eigene Gene sichern müssen. Menschen entwickeln kulturelle Attraktionen des Tötens wie Völkermord, Töten aus Hass, Rache oder Machtgewinn. Die Abwehr von Attraktionen des Tötens im Pazifismus und des Tötens von Tieren, sogar von Pflanzen, sind Anregungen für Wege in eine Globalgesellschaft.

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12

Kim, Min Jung, Jung Wan Kang, Ji Hyun Lee, Kyung Won Kim, Myung Hyun Sohn, Min Goo Lee, Myung-Joon Kim, and Kyu-Earn Kim. "A case Report of a Classic Cystic fibrosis Pediatric Patient in Korea Carrying Very Rare CFTR Gene Mutations (D993Y and Q220X)." Pediatric Allergy and Respiratory Disease 21, no. 1 (2011): 61. http://dx.doi.org/10.7581/pard.2011.21.1.61.

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13

Lerner, Terry J. "Scotland Funds Biotechnology Research Park." Genetic Engineering & Biotechnology News 31, no. 16 (September 15, 2011): 14. http://dx.doi.org/10.1089/gen.31.16.04.

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14

Sadhukhan, Susanta, Silpita Paul, Biswabandhu Bankura, Dinesh Munian, Sudakshina Ghosh, and Madhusudan Das. "Genetic analysis of MTR and MTRR gene polymorphisms in healthy mothers from Eastern part of India." International Journal of Research and Development in Pharmacy & Life Sciences 7, no. 1 (January 25, 2018): 2896–900. http://dx.doi.org/10.21276/ijrdpl.2278-0238.2018.7(1).2896-2900.

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15

Mead, Ian, James Apted, and Saphina Sharif. "Delivering London 2012: Contaminated soil treatment at the Olympic Park." Proceedings of the Institution of Civil Engineers - Geotechnical Engineering 166, no. 1 (February 2013): 8–17. http://dx.doi.org/10.1680/geng.11.00109.

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16

Marchard, S. P. "Discussion: Measurement of temporary prop loads at Mayfair car park." Proceedings of the Institution of Civil Engineers - Geotechnical Engineering 149, no. 4 (October 2001): 269. http://dx.doi.org/10.1680/geng.2001.149.4.269.

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17

Quisel, John D., and Alan D. Grossman. "Control of Sporulation Gene Expression in Bacillus subtilis by the Chromosome Partitioning Proteins Soj (ParA) and Spo0J (ParB)." Journal of Bacteriology 182, no. 12 (June 15, 2000): 3446–51. http://dx.doi.org/10.1128/jb.182.12.3446-3451.2000.

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ABSTRACT Two chromosome partitioning proteins, Soj (ParA) and Spo0J (ParB), regulate the initiation of sporulation in Bacillus subtilis. In a spo0J null mutant, sporulation is inhibited by the action of Soj. Soj negatively regulates expression of several sporulation genes by binding to the promoter regions and inhibiting transcription. All of the genes known to be inhibited by Soj are also activated by the phosphorylated form of the transcription factor Spo0A (Spo0A∼P). We found that, in a spo0J null mutant, Soj affected sporulation, in part, by decreasing the level of Spo0A protein. Soj negatively regulated transcription ofspo0A and associated with the spo0A promoter region in vivo. Expression of spo0A from a heterologous promoter in a spo0J null mutant restored Spo0A levels and partly bypassed the sporulation and gene expression defects. Soj did not appear to significantly affect phosphorylation of Spo0A. Thus, in the absence of Spo0J, Soj inhibits sporulation and sporulation gene expression by inhibiting accumulation of the activator protein Spo0A and by acting downstream of Spo0A to inhibit gene expression directly.

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18

Anjos, Taís Ramalho dos, Maria Júlia Sudária, Vinícius Silva Castro, Eduardo Eustáquio de Souza Figueiredo, and Ricardo César Tavares Carvalho. "The Rv2807 target gene: a determining factor to directly detect Mycobacterium bovis from suspected bovine tuberculosis lesions." Acta Veterinaria Brasilica 16, no. 4 (December 9, 2022): 309–12. http://dx.doi.org/10.21708/avb.2022.16.4.10988.

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Bovine tuberculosis (bTB) is a zoonosis caused by Mycobacterium bovis, a species belonging to the Mycobacterium tuberculosis complex (MTC) group. Direct bTB diagnosis from suggestive lesions can be performed by nested q-PCR targeting the Rv2807 gene present in the MTC group, as well as the TbD1 gene, present in M. bovis. In this context, the aim of the present study was to assess the importance of considering positive MTC results for the Rv2807 target gene obtained through the nested real time polymerase chain reaction (nested q-PCR) applied to samples obtained directly from suspected bTB lesions. A total of 174 samples of suggestive bTB caseous lesions were obtained during cattle slaughter in slaughterhouses in the state of Mato Grosso, Brazil. DNA was extracted from the lesions and nested q-PCR was performed to detect both MTC and M. bovis. Both samples positive for the Rv2807 (41/174) and TbD1 (29/174) were submitted to bacterial culturing (23/41), and the DNA of the isolates (23) was extracted and submitted again to nested q-PCR. The Rv2807 gene (MTC) was previously amplified by nested q-PCR directly from the lesions, although the TbD1 gene specific for M. bovis was not amplified previously in four of the successfully isolated samples (4/23), only following isolation, and only the Rv2807 gene was amplified before and after isolation. In conclusion, the target gene Rv2807(MTC) exhibited higher positivity in the analyzed samples compared to the TbD1 gene (M. bovis).

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UCHIUMI, FUMIAKI, TAKESHI WATANABE, RYO OHTA, HIDEAKI ABE, and SEI-ICHI TANUMA. "PARP1 gene expression is downregulated by knockdown of PARG gene." Oncology Reports 29, no. 5 (March 4, 2013): 1683–88. http://dx.doi.org/10.3892/or.2013.2321.

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20

MIYAMOTO, Hitoshi, and Mitsuharu MURASE. "Mutations of gyrA gene and parC gene in Streptococcus pneumoniae." Journal of the Japanese Association for Infectious Diseases 77, no. 3 (2003): 133–37. http://dx.doi.org/10.11150/kansenshogakuzasshi1970.77.133.

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21

Jeong, Kyongyeon, and Byungseol Byun. "A Study of Ecotourism Activated Gede Pangrango National Park in Indonesia." Journal of Wetlands Research 15, no. 1 (February 28, 2013): 139–47. http://dx.doi.org/10.17663/jwr.2013.15.1.139.

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22

Philippidis, Alex. "DANAHER DELIVERS: Cytiva, Pall Plan $1.5B Manufacturing Expansion." Genetic Engineering & Biotechnology News 41, no. 10 (October 1, 2021): 24–25. http://dx.doi.org/10.1089/gen.41.10.08.

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23

Wu, Kuan, Lei Xu, and Ling Cheng. "PAR2 Promoter Hypomethylation Regulates PAR2 Gene Expression and Promotes Lung Adenocarcinoma Cell Progression." Computational and Mathematical Methods in Medicine 2021 (April 15, 2021): 1–10. http://dx.doi.org/10.1155/2021/5542485.

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Objective. Protease-activated receptor-2 (PAR2) also known as F2RL1 is a G protein-coupled receptor that intimately correlates with cancer occurrence. DNA methylation turns out a vital mechanism regulating gene expression, while PAR2 promoter methylation is proven to be involved in cancer development. Hence, this study attempted to clarify the molecular mechanism by which PAR2 mediates lung adenocarcinoma (LUAD) progression, via identifying the effect of PAR2 promoter methylation on LUAD cell progression. Methods. Associations of PAR2 promoter methylation with PAR2 gene expression and prognosis of LUAD patients were analyzed via bioinformatics analysis. PAR2 promoter methylation and gene expression at the cellular level were measured using methylation-specific PCR, qRT-PCR, and Western blot assays. DNA methyltransferase inhibitor 5-AzadC was used to treat cells to assess PAR2 gene expression alteration. Cell biological behaviors upon PAR2 overexpression were characterized via MTT, wound healing assay, and Transwell assay. Results. Bioinformatics analysis revealed that PAR2 promoter methylation was negatively related to PAR2 gene expression, while PAR2 promoter hypermethylation and low gene expression indicated favorable LUAD prognosis. Besides, it turned out that PAR2 presented upregulated expression and hypomethylated promoter in LUAD cells. Moreover, PAR2 gene expression was elevated in cells treated with 5-AzadC, and the proliferative, migratory, and invasive capabilities of cells with 5-AzadC or high PAR2 gene expression were all enhanced. Conclusion. In sum, PAR2 promoter hypomethylation potentiates LUAD cell progression, in turn affecting the prognosis of LUAD patients.

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Lockman, B., S. Mascheretti, S. Schechter, and M. Garbelotto. "A First Generation Heterobasidion Hybrid Discovered in Larix lyalli in Montana." Plant Disease 98, no. 7 (July 2014): 1003. http://dx.doi.org/10.1094/pdis-12-13-1211-pdn.

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On September 25, 2010, a wood sample was collected from an entirely decayed root ball of an alpine larch (Larix lyallii Parl.), 10 cm in diameter at breast height, recently downed, but still green. No attempts were made to determine whether the decay progressed into the stem. The discovery occurred in a stand in the Bitterroot Mountains, south of Darby, Montana (elev. 2,530 m; 45.893528° N, 114.278322° W). Several adjacent alpine larches were either dead or displayed thin crowns, and an old Heterobasidion basidiocarp was found on the decayed root ball of a neighboring dead tree, suggesting the presence of a root disease pocket. The stand is mature and composed of alpine larch, whitebark pine (Pinus albicaulis Engelm.), and a few subalpine firs (Abies lasiocarpa (Hooker) Nuttall), but only larches were symptomatic. No stumps were visible, and the site is in a designated wilderness area characterized by minimal forest management. Wood chips displaying a white rot with bleached speckles were plated on 2% malt agar, and cultures displaying the typical Heterobasidion anamorph (Spiniger meineckellus) were visible after 7 days. DNA was extracted from two distinct cultures, and the sequences of three nuclear loci, namely the internal transcribed spacer, the elongation factor 1-alpha, and the glyceraldehyde 3-phosphate dehydrogenase, were analyzed. The sequence of the mitochondrial ATPase was also sequenced. All loci were amplified using the primers indicated in Linzer et al. (2). Sequences of all three nuclear loci (GenBank Accession Nos. KF811480 to 82) unequivocally indicated both isolates to be first generation hybrids between H. irregulare (Underw.) Garbel. & Otrosina and H. occidentale Otrosina & Garbel. Cumulatively, sequences were heterozygous at over 40 positions in all three loci, and for the presence of two indels (one in ITS, one in EF 1-alpha). Polymorphisms and indels indicated alleles from both species were present in these heterokaryotic (ploidy n+n) isolates. The mitochondrial ATPase (KF811483 to 84) indicated instead the cytoplasm belonged to H. occidentale, suggesting that species was the first to be established in the infected tree and was either dikaryotized by a basidiopsore of the other species, or subject to nuclear re-assortment through di-mon mating with a genotype of H. irregulare. This is the first report of a Heterobasidion sp. in L. lyalli, and it is the second report of a natural Heterobasidion hybrid in North America (1). This finding indicates Alpine larch may be a host for both Heterobasidion species, as described for pine stumps in California (1). Thus, this conifer may have provided a substrate for the hybridization and interspecific gene introgression documented to have occurred before stumps were generated in high frequency by modern forestry practices (2). References: (1) M. Garbelotto et al. Phytopathology 86:543, 1996. (2) R. Linzer et al. Mol. Phylogenet. Evol. 46:844, 2008.

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Tasaki, Yoko, Sachiyo Fukuda, Masayoshi Iio, Retsu Miura, Toshio Imai, Sumio Sugano, Osamu Yoshie, Austin L. Hughes, and Hisayuki Nomiyama. "Chemokine PARC Gene (SCYA18) Generated by Fusion of Two MIP-1α/LD78α-like Genes." Genomics 55, no. 3 (February 1999): 353–57. http://dx.doi.org/10.1006/geno.1998.5670.

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26

Wahbi, Hassan. "Unzählbare Schönheit. Für eine Poetik des Interkulturellen." Paragrana 20, no. 2 (December 2011): 189–95. http://dx.doi.org/10.1524/para.2011.0044.

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ZusammenfassungIn diesem Beitrag möchte ich zurückkommen auf die Frage nach der Erweiterung des Ich im Laufe eines Lebens, welches mehrfältig ist oder wird; es ist dies eine Variante dessen, was ich schon in früheren Texten vorschlug (L’art dialogique, Alexandria 2006; L’apprentissage négatif, Tunis 2008; Hybride toi-même, Casablanca 2009) bezüglich der notwendigen Labilität des Ich, d. h., dass der mehrfältige Mensch nicht mehrfältig ist in seiner Dispersion, sondern im Erarbeiten seiner Redefinition. Alles neu Erworbene bedingt, was er ist und stellt ihn vor etwas Weiteres. Eine Art der Vervollkommnungsmöglichkeit auf persönlichem Niveau, nicht moralisch, sondern eher als Vision der Dinge und des Selbst. Dieses Mal gehe ich von einer weniger verdeckten Subjektivität aus, falls ich das je zu tun versäumte, denn Erfahrung speist sich ja ununterbrochen aus einer interkulturellen Wunde, die sich als Sollbruchstelle träumt.

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27

Franqui-Rivera, Harry. "National Mythologies: U.S. Citizenship for the People of Puerto Rico and Military Service." Memorias 21 (May 12, 2022): 5–21. http://dx.doi.org/10.14482/memor.21.564.122.

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That Puerto Ricans became American citizens in 1917 have been attributed by many to the need for soldiers as the U.S. entered the First World War. Such belief has been enshrined in Puerto Rican popular national mythology. While there is a rich body of literature surrounding the decision to extend U.S. citizenship to Puerto Rico and its effect on the Puerto Ricans, few, if any, challenge the assumption that the need for manpower for the armies of the metropolis influenced that decision. Reducing the issue of citizenship to a need for manpower for the military o nly o b s c ures c o mp lex imp erial-colonial relations based upon racial structures of power. In this essay I hope to demonstrate that the need for soldiers was unrelated to the granting of citizenship in 1917. As the U.S. prepared for war, domestic politics and geopolitics were mostly responsible for accelerating the passing of the Jones Act.

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Rozak, Andes Hamuraby, Sri Astutik, Zaenal Mutaqien, Didik Widyatmoko, and Endah Sulistyawati. "KEKAYAAN JENIS POHON DI HUTAN TAMAN NASIONAL GUNUNG GEDE PANGRANGO, JAWA BARAT." Jurnal Penelitian Hutan dan Konservasi Alam 13, no. 1 (2016): 1–14. http://dx.doi.org/10.20886/jphka.2016.13.1.1-14.

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Tonelli, Fernanda M. P., and Rodrigo R. Resende. "PROFESSOR XAVIER: Usando O Poder Da Mente Para Controlar A Expressão De Genes." Nanocell News 2, no. 8 (February 24, 2015): n/a. http://dx.doi.org/10.15729/nanocellnews.2015.02.24.005.

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Gao, Lin, Ian G. Macara, and Gérard Joberty. "Multiple splice variants of Par3 and of a novel related gene, Par3L , produce proteins with different binding properties." Gene 294, no. 1-2 (July 2002): 99–107. http://dx.doi.org/10.1016/s0378-1119(02)00681-9.

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Satoh, Yasuharu, Masahiro Kuratsu, Daiki Kobayashi, and Tohru Dairi. "New gene responsible for para-aminobenzoate biosynthesis." Journal of Bioscience and Bioengineering 117, no. 2 (February 2014): 178–83. http://dx.doi.org/10.1016/j.jbiosc.2013.07.013.

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Meyer, Judith L., and Yolonda Youngs. "Historical Landscape Change in Yellowstone National Park: Demonstrating the Value of Intensive Field Observation and Repeat Photography." Geographical Review 108, no. 3 (July 1, 2018): 387–409. http://dx.doi.org/10.1111/gere.12255.

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Guan, Ping, Arthur H. M. Burghes, Anne Cunningham, Paul Lira, William H. Brissette, Kuldeep Neote, and Shaun R. McColl. "Genomic Organization and Biological Characterization of the Novel Human CC Chemokine DC-CK-1/PARC/MIP-4/SCYA18." Genomics 56, no. 3 (March 1999): 296–302. http://dx.doi.org/10.1006/geno.1998.5635.

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Vásquez-Marín, Belkys, Saúl Salazar-Sequea, Oscar De La Rosa, Omar Verde, Alexis Marques-Urdaneta, and Lourdes Tibisay Vilanova-Fernández. "Asociación de polimorfismos del gen Leptina con calidad seminal en toros raza Carora." Revista Científica de la Facultad de Ciencias Veterinarias XXXI, no. 4 (September 22, 2021): 147–56. http://dx.doi.org/10.52973/rcfcv-luz314.art4.

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La presencia de toros con características seminales deficientes ocasiona fallas considerables en la eficiencia reproductiva de las unidades de producción, debido a la disminución del número de vacas preñadas, siendo más marcado en sistemas bajo programas de inseminación artificial (IA). Los rasgos de calidad seminal (CS) pueden constituir un criterio importante para la selección de machos reproductores utilizados en IA. La identificación de marcadores moleculares asociados con CS en el toro, podría facilitar la selección para estos rasgos. Este estudio tuvo como objetivo evaluar la asociación de los polimorfismos del gen Leptina, sobre la CS de toros de la raza Carora. Se evaluaron las variables volumen de eyaculado (VE), motilidad masal (MM), motilidad individual (MI) y concentración espermática (CE) de 43 toros reproductores Carora, organizados en 8 grupos de edad. Los polimorfismos nucleotídicos simples (SNP) del gen Leptina evaluados fueron: rs29004487 (SNP1), rs29004488 (SNP2), rs29004501 (SNP3) y rs29004508 (SNP4). Se utilizó un análisis de varianza mediante un modelo lineal generalizado (GLM). El factor genotipo contó con 10 niveles y el factor edad, 8 niveles. No se observó efecto del SNP1 sobre ninguna de las variables evaluadas, mientras que el SNP3 tuvo un efecto significativo sobre la CE. Los SNP2 y SNP4 presentaron un efecto altamente significativo sobre la MI y CE. Finalmente, las variables VE y MM no fueron afectadas por ninguno de los SNP estudiados. Los resultados del presente estudio sugieren marcadores potenciales para la valoración y selección genética de bovinos reproductores.

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Urdiana-Arteaga, Estefania del Carmen, and Edgar García-López. "Análisis bioinformático de la ruta de biosíntesis de fenilpropanoides en Gomphrena globosa para el estudio de los niveles de expresión génica." Mexican Journal of Biotechnology 3, no. 3 (July 1, 2018): 68–76. http://dx.doi.org/10.29267/mxjb.2018.3.3.68.

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Gomphrena globosa es una planta que produce diferentes metabolitos secundarios que son de interés debido a la actividad biológica que presentan, por ejemplo: actividad antiinflamatoria, citotóxica, antioxidante y antimicrobiana. Los principales metabolitos que se han encontrado en cultivos in vitro son fenoles simples y flavonoides. La ruta biosintética de estos compuestos es bien conocida, siendo útil como herramienta para manipulación genética al existir amplia información sobre sus genes estructurales. Sin embargo, no hay suficiente información sobre su regulación, lo que abre campo para investigar la correlación entre los niveles de expresión de los genes correspondientes y la regulación de la ruta en G. globosa, con aplicaciones en la ingeniería metabólica. Por lo tanto, se realizó un análisis bioinformático para identificar posibles enzimas candidatas que ejerzan regulación o que fueran puntos de ramificación en la ruta. Las secuencias de nucleótidos se obtuvieron de enzimas altamente relacionadas, debido a que no hay secuencias reportadas de enzimas de G. globosa al momento, sin embargo la información disponible para estas moléculas relacionadas permitió seleccionar un grupo de seis enzimas como nodos de regulación en la vía biosintética de fenilpropanoides en G. globosa. Los oligonucleótidos diseñados por homología de secuencia reunieron las consideraciones de diseño para ser utilizados en subsecuentes análisis de expresión génica y son un importante aporte a la investigación genética y de regulación metabólica en G. globosa.

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Balsalobre, Luz, María José Ferrándiz, Josefina Liñares, Fe Tubau, and Adela G. de la Campa. "Viridans Group Streptococci Are Donors in Horizontal Transfer of Topoisomerase IV Genes to Streptococcus pneumoniae." Antimicrobial Agents and Chemotherapy 47, no. 7 (July 2003): 2072–81. http://dx.doi.org/10.1128/aac.47.7.2072-2081.2003.

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ABSTRACT A total of 46 ciprofloxacin-resistant (Cipr) Streptococcus pneumoniae strains were isolated from 1991 to 2001 at the Hospital of Bellvitge. Five of these strains showed unexpectedly high rates of nucleotide variations in the quinolone resistance-determining regions (QRDRs) of their parC, parE, and gyrA genes. The nucleotide sequence of the full-length parC, parE, and gyrA genes of one of these isolates revealed a mosaic structure compatible with an interspecific recombination origin. Southern blot analysis and nucleotide sequence determinations showed the presence of an ant-like gene in the intergenic parE-parC regions of the S. pneumoniae Cipr isolates with high rates of variations in their parE and parC QRDRs. The ant-like gene was absent from typical S. pneumoniae strains, whereas it was present in the intergenic parE-parC regions of the viridans group streptococci (Streptococcus mitis and Streptococcus oralis). These results suggest that the viridans group streptococci are acting as donors in the horizontal transfer of fluoroquinolone resistance genes to S. pneumoniae.

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Sawitzke, James A., Yongfang Li, Kirill Sergueev, Brenda Youngren, Therese Brendler, Kristine Jones, and Stuart Austin. "Transcriptional Interference by a Complex Formed at the Centromere-Like Partition Site of Plasmid P1." Journal of Bacteriology 184, no. 9 (May 1, 2002): 2447–54. http://dx.doi.org/10.1128/jb.184.9.2447-2454.2002.

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ABSTRACT The partition site, parS, promotes accurate segregation of the replicated P1 plasmid to daughter cells when the P1-encoded ParA and ParB proteins are supplied. The parS site was inserted into the Escherichia coli chromosome between the promoter and the structural gene for β-galactosidase, lacZ. There was little interference with lacZ expression when ParA and ParB were supplied in trans. However, when a mutant ParA protein, ParAM314I, was supplied along with ParB, expression of lacZ was shut down. ParAM314I, ParB, and parS appear to form a nucleoprotein complex that blocks transcription. Mutations in parA and parB that relieved the parAM314I-dependent block were found. In addition, new mutations which impose the block were selected. Five of the latter mapped to parA and one to parB; all had a propagation-defective phenotype (ParPD) similar to that of parAM314I. Thus, whereas a null par mutant P1 plasmid segregates its DNA randomly, these mutants prevent even random distribution of the plasmid. We propose that ParA protein normally interacts transiently with the ParB-parS complex for partition to proceed but that the mutations block ParA dissociation. This “permanent” ParA-ParB-parS complex acts as a transcription block. Consistent with this hypothesis, we found that three of the seven blocking mutations lie within regions of ParA and ParB that are known to interact with each other. When the transcription block is imposed, regional silencing of nearby genes occurs. However, the requirement for ParA and a mutant parA or parB allele distinguishes the transcription block from the regional ParB-dependent gene silencing previously described.

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Santos, Luana Oliveira dos, Juliana Vieira de Barros, Bethânia de Araújo Silva Amaral, Nara Diniz Soares Pessôa, João Bosco de Oliveira Filho, Andréa de Rezende Duarte, Jacqueline Araújo, Bárbara Gomes, and Neide Santos. "Investigação de deleções e duplicações do gene SHOX e regiões regulatórias em pacientes portadoras de baixa estatura." Semina: Ciências Biológicas e da Saúde 38, no. 1supl (February 16, 2018): 193. http://dx.doi.org/10.5433/1679-0367.2017v38n1suplp193.

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O padrão de crescimento é um dos melhores indicadores do estado de saúde da criança, sendo fundamental para a formação de um indivíduo adulto saudável. A baixa estatura (BE), por definição, é altura inferior a 2 desvios padrões (DP) abaixo da média para o sexo e idade, que é demonstrada na curva padrão de crescimento como um comprimento ou altura inferior ao percentil 3. Um dos mais importantes genes candidato para investigação em indivíduos com baixa estatura é o gene SHOX (short-stature homeobox gene), localizado na região pseudoautossômica 1 (PAR1), nos cromossomos sexuais X (Xp22.3) e Y (Yp11.3). Desta forma, este trabalho teve como objetivo Investigar deleções/duplicações do gene SHOX e suas regiões regulatórias em pacientes com cariótipo normal (46, XX) que apresentaram sinais clínicos de baixa estatura associada ou não a malformações esqueléticas. As pacientes foram provenientes do Serviço de Genética Médica do IMIP e do Serviço de Endocrinologia Pediátrica do HC. A análise citogenética através do bandeamento G foi realizada em linfócitos de sangue periférico e a investigação de deleções e duplicações do gene SHOX e regiões regulatórias foi realizada através da técnica de MLPA (Multiplex Ligation-Dependent Probe Amplification). A análise por citogenética clássica não detectou alterações cromossômicas em 26 pacientes, as quais apresentaram cariótipo 46, XX em todas as células analisadas. As análises por MLPA realizadas nestas pacientes identificaram rearranjos em três indivíduos. Uma paciente revelou ausência de todo os éxons do gene SHOX, apresentando também uma deleção do gene PPP2R3B-5, localizado na PAR1 (Xp22), bem como deleções de elementos não-codificantes conservados (CNEs), elementos regulatórios localizados “upstream” e “downstream” do gene SHOX. Em outro indivíduo, uma deleção foi detectada na região Xp22-PAR1, na área do gene SHOX. Outra paciente apresentou duplicação do gene ASMT-8, localizado PAR1 (Xp22). Os resultados obtidos indicam que a investigação citogenética descartou rearranjos estruturais envolvendo o braço curto do cromossomo X e que o uso da técnica de MLPA detectou alterações do gene SHOX e regiões regulatórias deste gene. Assim, um diagnóstico genético mais preciso pôde ser estabelecido paras as pacientes com BE, as quais poderão ser encaminhadas a um tratamento adequado.Auxílio Financeiro: FACEPE, CAPES

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Syahadat, Epi. "FAKTOR - FAKTOR YANG MEMPENGARUHI KUNJUNGAN WISATAWAN DI TAMAN NASIONAL GEDE PANGRANGO (TNGP)." Jurnal Penelitian Sosial dan Ekonomi Kehutanan 3, no. 1 (March 31, 2006): 17–40. http://dx.doi.org/10.20886/jpsek.2006.3.1.17-40.

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Brandão, Flávia Vieira, Cláudia Márcia Resende Silva, Bernardo Gontijo, and Antônio Carlos Martins Guedes. "Caso para diagnóstico." Anais Brasileiros de Dermatologia 84, no. 6 (December 2009): 677–79. http://dx.doi.org/10.1590/s0365-05962009000600017.

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Fibromatose hialina juvenil e hialinose sistêmica infantil são desordens autossômicas recessivas, raras da infância. À histologia, há depósito de material hialino na derme e subcutâneo. As características clínicas principais são: lesões pápulo-nodulares, hipertrofia gengival, contratura articular, lesões ósseas osteolíticas e retardo no crescimento. Mutações no mesmo gene foram identificadas nas duas condições, sugerindo que sejam espectros da mesma doença.

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Takahashi, Y., and T. Nagata. "parB: an auxin-regulated gene encoding glutathione S-transferase." Proceedings of the National Academy of Sciences 89, no. 1 (January 1, 1992): 56–59. http://dx.doi.org/10.1073/pnas.89.1.56.

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Hora, João Sergio Ignacio, Ivan Orlando Castellanos Rangel, Patrícia Alexandra Viteri Rodas, and Guilherme de Lima Pinheiro. "Distrofia muscular de cinturas - doença rara desafiadora para o médico e para o paciente." Acta Fisiátrica 29, Supl.1 (November 30, 2022): S42—S43. http://dx.doi.org/10.11606/issn.2317-0190.v29isupl.1a204930.

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A distrofia muscular de cintura/Limb Girdle Muscular Distrophy (DMC/LGMD) é causada por mutações do gene CAPN3 que codifica a proteína Calpaína, que desempenha um papel na manutenção da integridade e função muscular.

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Salzano, Francisco M. "Gene HBB." Genética na Escola 7, no. 2 (March 10, 2012): 90–95. http://dx.doi.org/10.55838/1980-3540.ge.2012.140.

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É feita uma revisão geral sobre a anemia falciforme e a relação entre a mutação (S) que condiciona a produção da cadeia beta da hemoglobina S (HbS) com a proteção à malária. Salienta-se que as doenças que afetam a hemoglobina (hemoglobinopatias) constituem- se em um problema de saúde pública no Brasil, fornecendo-se resultados sobre as frequências de indivíduos heterozigotos AS e homozigotos SS em seis populações brasileiras, obtidas através do Programa Nacional de Triagem Neonatal. Apresentam-se as justifi cativas para o estabelecimento de tais programas, fornecendo-se posteriormente informações sobre outro tipo de hemoglobina, a Hb Porto Alegre. A seção final aborda a questão da importância do conhecimento genético e da ciência em geral para o bem-estar e a felicidade dos seres humanos.

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Akasaka, Takaaki, Yoshikuni Onodera, Mayumi Tanaka, and Kenichi Sato. "Cloning, Expression, and Enzymatic Characterization of Pseudomonas aeruginosaTopoisomerase IV." Antimicrobial Agents and Chemotherapy 43, no. 3 (March 1, 1999): 530–36. http://dx.doi.org/10.1128/aac.43.3.530.

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ABSTRACT The topoisomerase IV subunit A gene, parC homolog, has been cloned and sequenced from Pseudomonas aeruginosa PAO1, with cDNA encoding the N-terminal region of Escherichia coli parC used as a probe. The homolog and its upstream gene were presumed to be parC and parE through sequence homology with the parC and parE genes of other organisms. The deduced amino acid sequence of ParC and ParE showed 33 and 32% identity with that of the P. aeruginosa DNA gyrase subunits, GyrA and GyrB, respectively, and 69 and 75% identity with that of E. coli ParC and ParE, respectively. The putative ParC and ParE proteins were overexpressed and separately purified by use of a fusion system with a maltose-binding protein, and their enzymatic properties were examined. The reconstituted enzyme had ATP-dependent decatenation activity, which is the main catalytic activity of bacterial topoisomerase IV, and relaxing activities but had no supercoiling activity. So, the cloned genes were identified asP. aeruginosa topoisomerase IV genes. The inhibitory effects of quinolones on the activities of topoisomerase IV and DNA gyrase were compared. The 50% inhibitory concentrations of quinolones for the decatenation activity of topoisomerase IV were from five to eight times higher than those for the supercoiling activities ofP. aeruginosa DNA gyrase. These results confirmed that topoisomerase IV is less sensitive to fluoroquinolones than is DNA gyrase and may be a secondary target of new quinolones in wild-typeP. aeruginosa.

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Jiang, Wei, and Richard L. Hallberg. "Isolation and Characterization of par1+ and par2+: Two Schizosaccharomyces pombe Genes Encoding B′ Subunits of Protein Phosphatase 2A." Genetics 154, no. 3 (March 1, 2000): 1025–38. http://dx.doi.org/10.1093/genetics/154.3.1025.

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Abstract Protein phosphatase 2A (PP2A) is one of the major serine/threonine phosphatases found in eukaryotic cells. We cloned two genes, par1+ and par2+, encoding distinct B′ subunits of PP2A in fission yeast. They share 52% identity at the amino acid sequence level. Neither gene is essential but together they are required for normal septum positioning and cytokinesis, for growth at both high and low temperature, and for growth under a number of stressful conditions. Immunofluorescence microscopy revealed that Par2p has a cell-cycle-related localization pattern, being localized at cell ends during interphase and forming a medial ring in cells that are undergoing septation and cytokinesis. Our analyses also indicate that Par1p is more abundant than Par2p in the cell. Cross-organism studies showed that both par1+ and par2+ could complement the rts1Δ allele in Saccharomyces cerevisiae, albeit to different extents, in spite of the fact that neither contains a serine/threonine-rich N-terminal domain like that found in the S. cerevisiae homolog Rts1p. Thus, while Schizosaccharomyces pombe is more similar to higher eukaryotes with respect to its complement of B′-encoding genes, the function of those proteins is conserved relative to that of Rts1p.

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Yin, Ping, Tai-Yuan Li, Mao-Hua Xie, Lina Jiang, and Yi Zhang. "A Type Ib ParB Protein Involved in Plasmid Partitioning in a Gram-Positive Bacterium." Journal of Bacteriology 188, no. 23 (September 22, 2006): 8103–8. http://dx.doi.org/10.1128/jb.01232-06.

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ABSTRACT Our current understanding of segregation of prokaryotic plasmids has been derived mainly from the study of the gram-negative bacterial plasmids. We previously reported a replicon of the cryptic plasmid from a gram-positive bacterium, Leifsonia xyli subsp. cynodontis. The replicon contains a putative plasmid partition cassette including a Walker-type ATPase followed by open reading frame 4 without sequence homologue. Here we reported that the orf4 gene was essential for maintaining the plasmid stability in L. xyli subsp. cynodontis. Furthermore, the purified orf4 protein specifically and cooperatively bound to direct repeat sequences located upstream of the parA gene in vitro, indicating that orf4 is a parB gene and that the direct repeat DNA sequences constitute a partition site, parS. The location of parS and the features of ParA and ParB proteins suggest that this plasmid partition cassette belongs to type Ib, representing the first type Ib cassette identified from a gram-positive bacterial plasmid.

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Díaz-Ferreira, Natalia Jazmín, Mabel Rocío Miranda-Echagüe, María Alexandra Mercado Amarilla, Iara Magaly Martínez Pereira, Adriana Beatriz Valenzuela Cáceres, Laura Patricia Mendoza Torres, and Pamela Esther Mongelós Dacunte. "Optimización de una técnica de PCR convencional para la amplificación de la región LCR y el gen E6 del virus del papiloma humano tipo 16." Memorias del Instituto de Investigaciones en Ciencias de la Salud 20, no. 2 (August 1, 2022): 13–19. http://dx.doi.org/10.18004/mem.iics/1812-9528/2022.020.02.13.

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El cáncer de cuello uterino es el cuarto cáncer más frecuente en mujeres en el mundo y a nivel mundial, el VPH 16 se encuentra presente en aproximadamente el 60% de los casos. A la fecha, las variantes de VPH 16 se clasifican en 4 linajes y 16 sublinajes asociándose algunas variantes con severidad de lesión. La secuenciación de la región LCR y del gen E6 es utilizada para la clasificación de variantes. Por ello, el objetivo fue optimizar 2 PCRs convencionales para detectar la región LCR y una PCR para el gen E6. Para ello, se utilizaron muestras positivas para VPH 16, cebadores específicos para la región LCR y el gen E6. Se probaron las reacciones a diferentes temperaturas de anillamientos. La concentración de MgCl2, dNTP y cebadores fueron determinadas siguiendo las recomendaciones del fabricante de la enzima ADN polimerasa utilizada. Para la amplificación de la región LCR y el gen E6 del VPH 16, se observaron mejores resultados a una temperatura de anillamiento de 57°C y 50°C, respectivamente. La concentración de MgCl2 utilizada en ambas reacciones fue de 1,5mM, la de dNTP 0,2mM y la de cebadores 0,2uM. La optimización de la reacción de PCR permitirá obtener amplicones aptos para secuenciación, a fin de determinar las variantes génicas y posteriormente evaluar funcionalidad y actividad transcripcional que puedan estar relacionadas con la patogénesis cervical.

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Schindler, Larissa. "Eine Kampfkunst lernen: Didaktische Transformationen und somatische Kommunikation." Paragrana 25, no. 1 (June 1, 2016): 361–72. http://dx.doi.org/10.1515/para-2016-0019.

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AbstractSeit geraumer Zeit beschäftigt sich die Soziologie mit der Thematik des „Körperwissens“: Von Marcel Mauss’ These der Körpertechniken über Schütz’ Ausführungen zu Fertigkeiten, Bourdieus Thesen zum Habitus bis hin zu einer Reihe aktueller Arbeiten reicht der Bogen. Ich beschäftige mich im Folgenden mit dem Phänomen der schweigsamen Wissensvermittlung aus mikrosoziologischer Perspektive. Anhand von empirischem Material aus einer ethnografischen Studie in einem Kampfkunstverein gehe ich der Frage nach, wie in der alltäglichen Praxis des Trainings ein zum großen Teil im Medium des Körperlichen operierendes Wissen vom Trainer an die Schüler/-innen weitergegeben wird. Dabei zeigt sich, dass das körperliche Wissen der (inzwischen europäisch geprägten) Kampfkunst vor allem auf visuellem und somatischem Weg vermittelt wird. Körper führen Bewegungen vor und sie geben einander in Partnerübungen Feedback. Das Körperwissen der Kampfkunst wird peu à peu in einer Kommunikation zwischen Körpern (Goffman) weitergegeben, die jedoch nicht nur einen visuellen, sondern vor allem auch einen somatischen Kanal hat.

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Syahadat, Epi. "ANALISA STRATEGI PENGELOLAAN TAMAN NASIONAL GEDE PANGRANGO (TNGP) UNTUK PENGEMBANGAN PARIWISATA ALAM DI KAWASAN HUTAN." Jurnal Penelitian Sosial dan Ekonomi Kehutanan 3, no. 2 (June 30, 2006): 117–32. http://dx.doi.org/10.20886/jpsek.2006.3.2.117-132.

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50

Arizala-Quinto, E. D., G. Viteri, and F. M. Idrovo-Espín. "PARTIAL SEQUENCES OF THE GENE THAT CODIFIES FOR THE TRANSCRIPTION FACTOR VPHSFB1 IN Vasconcellea pubescens. FIRST REPORT." Journal of Basic and Applied Genetics 30, no. 1 (July 2019): 7–9. http://dx.doi.org/10.35407/bag.2019.xxx.01.01.

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Plant heat stress transcription factors (HSFs) are involved in the response to heat. In Arabidopsis thaliana the HSFs genes are completely identified, however there was no information available about these genes in Vasconcellea pubescens (Chamburo) until now. In this preliminary work we describe the VPHSFB1 gene of V. pubescens (gene expression evaluated by RT-PCR and the partial sequence) that was induced by the increment of temperature. From our results, VPHSFB1 could be used as a heat response marker gene in tropical species. Key words: Caricaceae, gene expression, heat.

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