Relevant bibliographies by topics / Fungal bioreactors

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Academic literature on the topic 'Fungal bioreactors'

Author: Grafiati
Published: 10 March 2023

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Journal articles on the topic "Fungal bioreactors"

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Kırdök, Onur, Berker Çetintaş, Asena Atay, İrem Kale, Tutku Didem Akyol Altun, and Elif Esin Hameş. "A Modular Chain Bioreactor Design for Fungal Productions." Biomimetics 7, no. 4 (October 27, 2022): 179. http://dx.doi.org/10.3390/biomimetics7040179.

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Plastic bag bioreactors are single-use bioreactors, frequently used in solid culture fermentation. This study developed plastic bag bioreactors with more effective aeration conditions and particular connection elements that yield sensors, environmental control, and modular connectivity. This bioreactor system integrates the bags in a chain that circulates air and moisture through filtered connections. Within the present scope, this study also aimed to reveal that cultures in different plastic bags can be produced without affecting each other. In this direction, biomass production in the modular chain bioreactor (MCB) system developed in this study was compared to traditional bag systems. In addition, contamination experiments were carried out between the bags in the system, and it was observed that the filters in the developed system did not affect the microorganisms in different bags.
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Burton, Stephanie G. "Development of bioreactors for application of biocatalysts in biotransformations and bioremediation." Pure and Applied Chemistry 73, no. 1 (January 1, 2001): 77–83. http://dx.doi.org/10.1351/pac200173010077.

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Biotransformation systems, whether used for environmentally benign biocatalysis of synthetic reactions, or bioremediation of pollutants, require suitable biocatalysts and suitable bioreactor systems with particular characteristics. Our research focuses on the bioconversion of organic compounds, many of which are industrial residues, such as phenols, poly-aromatic hydrocarbons, heterocyclic compounds, and polychlorinated biphenyls. The purpose of such biotransformations can be twofold: firstly, to remove them from effluents and convert them to less toxic forms, and secondly, to convert them into products with economic value. We conduct research in utilizing various isolated-enzyme and whole-cell biological agents; bioreactors, including novel membrane bioreactors, are used as a means of supporting/immobilizing, and hence applying, these biocatalysts in continuous systems. In addition, the enzyme systems are characterized biochemically, to provide information which is required in modification, adaptation, and scale-up of the bioreactors. The paper summarizes research on application of biofilms of fungal and bacterial cells and their enzymes, including hydrolases, polyphenol oxidase, peroxidase and laccase, in bioreactor systems including continuously operating membrane bioreactors.
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Kim, Chano H., Jonq H. Park, In S. Chung, Sung R. Kim, and Seung W. Lee. "ENHANCED ANTHOCYANIN PRODUCTION IN HAIRY ROOT CULTURE OF DAUCUS CAROTA BY FUNGAL ELICITOR." HortScience 27, no. 6 (June 1992): 694b—694. http://dx.doi.org/10.21273/hortsci.27.6.694b.

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Secondary metabolite production by plant cell culture has been become of interest because of its commercial value in use. However, cultured plant cells usually yield lower levels of secondary metabolites than those of intact plants. In order to improve the anthocyanin productivity in hairy root culture of Daucus carota, fungal elicitors from 8 species of Fungi were examined. Through the studies of fungal elicitors in this work, it was turned out that fungal elicitors were very effective to improve the yield of anthocyanin. Despite of its low yield of anthocyanin, high density culture of hairy roots is achieved in fluidized-bed bioreactor, Anthocyanin production in fluidized-bed bioreactor with fungal elicitor treatment was increased greatly. We are currently researching more detailed aeration effects and scale-up in air-lift bioreactors. And these studies could provide important data to establish mass production system for secondary metabolites.
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Moreira, M. T., G. Feijoo, and J. M. Lema. "Fungal Bioreactors: Applications to White-Rot Fungi." Reviews in Environmental Science and Bio/Technology 2, no. 2-4 (2003): 247–59. http://dx.doi.org/10.1023/b:resb.0000040463.80808.dc.

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Svensson, Sofie E., Ludmila Bucuricova, Jorge A. Ferreira, Pedro F. Souza Filho, Mohammad J. Taherzadeh, and Akram Zamani. "Valorization of Bread Waste to a Fiber- and Protein-Rich Fungal Biomass." Fermentation 7, no. 2 (June 3, 2021): 91. http://dx.doi.org/10.3390/fermentation7020091.

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Filamentous fungi can be used for the valorization of food waste as a value-added product. The goal of this study was the valorization of bread waste through fungal cultivation and the production of value-added products. The fungal cultivation was verified for upscaling from shake flasks to a bench-scale bioreactor (4.5 L) and a pilot-scale bioreactor (26 L). The fungus showed the ability to grow without any additional enzymes or nutrients, and it was able to consume a bread concentration of 4.5% (w/v) over 48 h. The biomass concentration in the shake flasks was 4.1 g/L at a 2.5% bread concentration, which increased to 22.5 g/L at a 15% bread concentration. The biomass concentrations obtained after 48 h of cultivation using a 4.5% bread concentration were 7.2–8.3 and 8.0 g/L in 4.5 and 26 L bioreactors, respectively. Increasing the aeration rate in the 4.5 L bioreactor decreased the amount of ethanol produced and slightly reduced the protein content of the fungal biomass. The initial protein value in the bread was around 13%, while the protein content in the harvested fungal biomass ranged from 27% to 36%. The nutritional value of the biomass produced was evaluated by analyzing the amino acids and fatty acids. This study presents the valorization of bread waste through the production of a protein- and fatty-acid-rich fungal biomass that is simultaneously a source of microfibers.
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Massadeh, Muhannad I., Khalid Fandi, Hanan Al-Abeid, Othman Alsharafat, and Khaled Abu-Elteen. "Production of Citric Acid by Aspergillus niger Cultivated in Olive Mill Wastewater Using a Two-Stage Packed Column Bioreactor." Fermentation 8, no. 4 (March 30, 2022): 153. http://dx.doi.org/10.3390/fermentation8040153.

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For building a sustainable fermentation process, it is essential to reduce dependence on natural resources and lower the amount of pollution that is created. The reuse of agro-industrial wastewater after possible treatment leads to the achievement of these goals concurrently. This study investigates the production of citric acid and the cellulase enzyme by A. niger cultivated in olive mill wastewater (OMW) using a loofa sponge-packed column bioreactor. The process was conducted under batch conditions using a single-stage packed bioreactor and under continuous operation using two-stage packed-column bioreactors. Citric acid and cellulase enzyme production were enhanced when the culture was supplied with cellulose. Employing loofa sponge slices for cell entrapment/immobilization improved the efficiency of the process. The maximum citric acid concentration achieved was 16 g/L with a yield (YCit.A/BOD) of 38.5% and a productivity of 2.5 g/L/day. When the process parameters were translated into continuous operation employing two loofa sponge-packed column bioreactors, citric acid production was improved significantly to 25 g/L in a steady-state period of 5 days at a production rate of 3.6 g/L/day and an allover yield (YCit.A/BOD) of 57.5%. Cellulases and reducing sugars were continuously supplied to the second-stage bioreactor by the first-stage bioreactor, which in turn enhanced fungal growth and citric acid production.
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Santos, Ana Laura, and D. Barrie Johnson. "Design and Operation of Empirical Manganese-Removing Bioreactors and Integration into a Composite Modular System for Remediating and Recovering Metals from Acidic Mine Waters." Applied Sciences 11, no. 9 (May 10, 2021): 4287. http://dx.doi.org/10.3390/app11094287.

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Packed bed bioreactors were used to remove soluble manganese from a synthetic mine water as the final stage of an integrated bioremediation process. The synthetic mine water had undergone initial processing using a sulfidogenic bioreactor (pH 4.0–5.5) which removed all transition metals present in elevated concentrations (Cu, Ni, Zn and Co) apart from manganese. The aerobic bioreactors were packed with pebbles collected from a freshwater stream that were coated with black-colored, Mn(IV)-containing biofilms, and their capacity to remove soluble Mn (II) from the synthetic mine water was tested at varying hydraulic retention times (11–45 h) and influent liquor pH values (5.0 or 6.5). Over 99% of manganese was removed from the partly processed mine water when operated at pH 6.5 and a HRT of 45 h. Molecular techniques (clone libraries and T-RFLP analysis) were used to characterize the biofilms and identified two heterotrophic Mn-oxidizing microorganisms: the bacterium Leptothrix discophora and what appears to be a novel fungal species. The latter was isolated and characterized in vitro.
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McFarland, M. J., X. J. Qiu, J. L. Sims, M. E. Randolph, and R. C. Sims. "Remediation of Petroleum Impacted Soils in Fungal Compost Bioreactors." Water Science and Technology 25, no. 3 (February 1, 1992): 197–206. http://dx.doi.org/10.2166/wst.1992.0093.

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The ability of the white rot fungus Phanerochaete chrysosporium to enhance the biotransformation of benzo(a)pyrene (B(a)P) in contaminated soils was evaluated in compost bioreactors. Radiolabelled114C and chemical mass balances were used to evaluate: 1) rate of disappearance of test compound; 2) mineralization; 3) formation of bound contaminant residue; and 4) treatment costs. Mineralization of B(a)P was found to be insignificant over the duration of test period. Moreover, no radioactivity was recovered in volatile organic traps indicating that transformation of B(a)P resulted in chemicals intermediates that remained associated with the compost matrix. Bound contaminant residue formation was found to be the major mechanism of B(a)P removal accounting for nearly 100% of the contaminant loss from the solvent extract (methylene chloride/acetone). A maximum rate of bound contaminant removal of 1.36 mg B(a)P/Kg soil-day was estimated in fungal inoculated system over the first thirty days of treatment. This was significantly different from the maximum rate of bound residue formation estimated in the noninoculated systems (0.83 mg B(a)P/Kg soil-day) over the same time period. After thirty days, the rate of bound residue formation decreased to near zero in the inoculated system while remaining constant in the noninoculated reactors. The decrease in bound residue formation coincided with decline in benzo(a)pyrene removal. Data suggest that fungal activity may have been reduced over time by nutrient limitation.
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Awad, Mohamed F., and M. Kraume. "Fungal diversity in activated sludge from membrane bioreactors in Berlin." Canadian Journal of Microbiology 57, no. 8 (August 2011): 693–98. http://dx.doi.org/10.1139/w11-056.

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The objective of this study was to evaluate the occurrence of fungi in aerobic and anoxic activated sludge from membrane bioreactors. Thirty-six samples from each aerobic and anoxic activated sludge were taken from two membrane bioreactors treating domestic wastewater. Over a period of 9 months, four samples from each plant were taken per month. The samples were prepared for count and identification of fungi. Sixty species belonging to 30 genera were collected from activated sludge samples under aerobic and anoxic conditions. In terms of fungal identification, under aerobic conditions Geotrichum candidum was found at 94.4% followed by Penicillium species at 80.6%, yeasts at 75.0%, and Trichoderma species at 50.0%; under anoxic conditions G. candidum at 86.1%, yeasts at 66.6%, and Penicillium species at 61.1% were the most prevalent. The results indicate that activated sludge is a habitat for growth and sporulation of different groups of fungi, both saprophytic and pathogenic.
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Guryanov, D. V., V. D. Khmyrov, Yu V. Guryanova, B. S. Trufanov, and V. B. Kudenko. "Processing technology and electrical decontamination of bedding manure and litter in ground trenches and bioreactors." IOP Conference Series: Earth and Environmental Science 845, no. 1 (November 1, 2021): 012155. http://dx.doi.org/10.1088/1755-1315/845/1/012155.

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Abstract The paper presents data on the effect of electric current on the quantitative index of Mucor and Bacillus fungal colonies in bedding litter. It was found that with an increase in direct current to 3A, the quantitative index (%) of Mucor fungal colonies sharply decreases from 45 to 3 (by 42%), and the quantitative index (%) of Bacillus fungal colonies decreases from 70 to 50 (by 20%). Fresh litter has high acidity, and it cannot be used without preliminary processing and electrical decontamination in ground trenches and bioreactors.
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Dissertations / Theses on the topic "Fungal bioreactors"

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Umstead, Russell Blake. "Development of Fungal Bioreactors for Water Related Treatment and Disinfection Applications." Thesis, Virginia Tech, 2016. http://hdl.handle.net/10919/72291.

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Wastewater, recycled irrigation water, and agricultural runoff can contain high levels of pathogenic bacteria, which pose a threat to human and ecosystem health. The use of a bioreactor containing mycelial mats of filamentous fungi is a novel treatment technology that incorporates physical, biological, and biochemical processes to remove bacterial pathogens from influent water. Although a relatively new concept, fungal bioreactors have demonstrated the ability to dramatically reduce fecal coliform bacteria in water, but no studies have attempted to explicitly identify the bacterial pathogen removal mechanisms exhibited by the fungi. This study evaluated several different species of fungi for use in fungal bioreactor systems and aimed to identify the modes of action responsible for the removal of bacterial pathogens. The species evaluated were Daedaleopsis confragosa, Pleurotus eryngii, and Piptoporus betulinus. Experimental results showed that all species of fungi assessed were capable of removing E. coli in a synthetic water solution. Significant concentrations of hydrogen peroxide, an antiseptic, were produced by all species of fungi evaluated. The fungal bioreactors containing P. eryngii produced the highest concentrations of hydrogen peroxide, generating a maximum concentration of 30.5 mg/l or 0.896 mM. This maximum value exceeds reported minimum concentrations required to demonstrate bacteriostatic and bactericidal effects when continually applied, providing evidence that a major bacterial removal mode of action is the production of antimicrobial compounds. In addition to its promising application to improve water quality, fungal bioreactors are a low cost and passive treatment technology. The development a hyper-functional system could be a have a substantial impact on the use of recycled irrigation water and on the water/wastewater treatment industry, for both municipal and agricultural wastewater.
Master of Science
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Fillis, Vernon William. "Design of a packed-bed fungal bioreactor : the application of enzymes in the bioremediation of organo-pollutants present in soils and industrial effluent." Thesis, Peninsula Technikon, 2001. http://hdl.handle.net/20.500.11838/910.

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Thesis (MTech (Chemical Engineering))--Peninsula Technikon, 2001
Certain fungi have been shown to excrete extracellular enzymes, including peroxidases, laccases, etc. These enzymes are useful for bioremediation of aromatic pollutants present in industrial effluents (Leukes, 1999; Navotny et aI, 1999). Leukes (1999) made recent significant development in the form of a capillary membrane gradostat (fungal) bioreactor that offers optimal conditions for the production of these enzymes in high concentrations. This system also offers the possibility for the polluted effluent to be treated directly in the bioreactor. Some operating problems relating to continuous production of the enzymes and scale-up of the capillary modules, were, however, indentified. In an attempt to solve the above-mentioned identified problems the research group at Peninsula Technikon considered a number of alternative bioreactor configurations. A pulsed packed bed bioreactor concept suggested by Moreira et at. (1997) was selected for further study. Their reactor used polyurethane pellets as the support medium for the fungal biofilm and relied upon pulsing of the oxygen supply and recycle of nutrient solution in order to control biomass accumulation. These authors reported accumulation due to the recycle of proteases that were believed to destroy the desired ligninases. We experimented with a similar concept without recycle to avoid backrnixing and thereby overcome protease accumulation. In our work, a maximum enzyme productivity of 456 Units.L1day·1 was attained. Since this was significantly greater than the maximum reported by Moreira et aI, 1997 (202 Units.L-1day-I) it appeared that the elimination of recycle had significant benefits. In addition to eliminating recycle we also used a length / diameter (L / D) ratio of 14: 1 (compared with 2.5: 1 used by Moreira et aI, 1997) in order to further reduce backrnixing. Residence time distributions were investigated to gain insight into mechanisms of dispersion in the reactor. It was found that the pulsed packed bed concept presented problems with regard to blockage by excess biomass. This led us to consider the advantages of a fluidized bed using resin beads. Accordingly, growth of fungi on resin beads in shake flasks was investigated with favorable results. An experimental program is proposed to further investigate the fluidized bed concept with a view to extending the operation time of the bioreactor. From our literature survey to date, packed bed fungal bioreactors are still the best reactor configuration for continuous production ofligninolytic enzymes. An interesting study of the application of laccases to the degradation of naphthalene and MTBE is described in an addendum to this thesis.
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Thongchul, Nuttha. "Lactic acid production by immobilized Rhizopus oryzae in a rotating fibrous bed bioreactor." Connect to this title online, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1104333442.

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Thesis (Ph. D.)--Ohio State University, 2005.
Title from first page of PDF file. Document formatted into pages; contains xviii, 246 p.; also includes graphics (some col.) Includes bibliographical references (p. 207-222).
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Bulkan, Gülru. "Valorization Of Whole Stillage With Filamentous Fungi Cultivation Using Membrane Bioreactors." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-26252.

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A significant by-product of bioethanol plants is whole stillage, commonly used to produce animal feed due to its nutritious value, has a potential to be used to produce various value-added products while eliminating a costly process step is an alternative approach. In this study, production and separation of additional ethanol, fungal biomass and enzyme were successfully achieved with the cultivation in membrane bioreactors in batch process condition. Process optimization studies regarding fermentation and filtration conditions were carried out. Up to 10.4 g/l ethanol per litre of used whole stillage can be produced in simultaneous saccharification and fermentation (SSF) condition without any pH adjustment and additional pretreatment step. Also, 50% diluted whole stillage provided 87% higher ethanol production comparing to non-diluted medium. Moreover, 71 % higher biomass production was obtained with the filtrate of 50% diluted whole stillage comparing to 25% diluted one. Considering the achieved results, a two-stage cultivation using SHF (Separate Hydrolysis and Fermentation) strategy in membrane bioreactors for separation of ethanol, lignin-rich stream, protein-rich fungal biomass and enzymes was proposed. The present thesis showed that the integration of filamentous fungi with membrane bioreactors can increase the range of products that can be produced from whole stillage.
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Andersson, Eva Lotta. "Analysis of Various Bioreactor Configurations for Heavy Metal Removal Using the Fungus Penicillium ochro-chloron." Digital WPI, 2000. https://digitalcommons.wpi.edu/etd-theses/814.

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Penicillium ochro-chloron (ATCC strain # 36741), a filamentous fungus with the capability for removing copper ions from aqueous solutions, was studied as a possible biological trap (biotrap) for remediation of heavy metal contaminants in industrial wastewaters. This research demonstrated that in shake flasks the fungus removed copper from surrogate wastewater with 100mg/L copper contamination by as much as 99%. These results did not translate to the bioreactor configuration of a packed bed column, as channeling occurred through the bed, shown by conductivity tracer studies. A fluidized bed configuration was studied and resulted in copper removal of 97%, with a capacity of 149 mg[Cu]/g dry weight biomass, under the conditions of 50% dissolved oxygen. For dissolved oxygen concentrations below the critical oxygen concentration for the fungus (20% saturation) there was minimal copper removal. Mixing studies in the fluidized bed reactor showed that the system was diffusion limited. Mathematical modeling using first order kinetics associated with diffusion limited reactions resulted in rate constants for Cu 2+ uptake of approximately 0.031 h -1 , which were dependent on the dissolved oxygen concentration. Modeling of the reaction with a second order kinetic equation showed that there are possibly factors regulating copper uptake besides oxygen. Electron microscopy showed that in some instances the copper removed was retained as large porous spherical extracellular precipitates. Energy Dispersive X-ray (EDX) analysis has shown similar complexes to be copper phosphate precipitates (Crusberg, 1994). Removal of heavy metal contaminants from wastewater discharge is a necessity for many industries, due to environmental concerns and federal regulations. The use of a biological system for the removal and recycling of heavy metals could prove more economical than currently used physio-chemical processes.
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Ricky, Ricky. "Uppskalning av en svampkaka : process från avfallsbröd med en ätlig svamp." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-23882.

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Stale bread contributes to the biggest volume of food waste in Sweden. Current method on recovering bread waste is by producing biogas or bioethanol. Despite advantages in the energy sector, the bread which still has relatively high quality could be recovered into new products with higher value, such as food for human consumption. Development of a product, termed ‘fungal cake’ by solid state fermentation on bread waste using Neurospora intermedia in small scale petri dishes have previously been successfully conducted. This study aims to scale up the production of fungal cake into bench scale production. Two systems using different bioreactors were used in this study. The first system operated in batch mode using a tray bioreactor, in which the effect of particle size, mixing, and bread loading were evaluated. The fermentation was conducted during 5 days. Bread crumb with a larger particle size of 2 mm resulted in similar outcomes as bread crumb with a smaller particle size of 0.5 mm in terms of CO2 evolution rate, cumulative CO2 production, starch, and protein content of the final product. However, larger particle size resulted in a more homogeneous growth of the fungus throughout the product, which is preferred. The presence of daily mixing had no significant effect compared to static condition for all measured variables. Thus, mixing could be introduced to promote product homogeneity. Likewise, bread loading had no significant effect on the measured variables, which implies that a higher productivity can be achieved using a higher bread loading. The second system operated in continuous mode using a newly developed continuous tubular bioreactor with product recycle. Two experiments, in which the residence time (48h and 24h) and recycle ratio (10/65 and 20/55) were conducted. Both experiments yielded product with stable starch and protein content, indicated by a stable CO2 evolution rate over time. The performance using continuous tubular bioreactor was compared to batch fermentation in tray bioreactor using the same ratio of inoculum and both system yielded product with the same starch and protein content. Successful operation in continuous bioreactor certainly improved the productivity of fungal cake production.
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Leukes, W. "Development and characterisation of a membrane gradostat bioreactor for the bioremediation of aromatic pollutants using white rot fungi." Thesis, Rhodes University, 1999. http://hdl.handle.net/10962/d1004092.

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Bioremediation of aromatic pollutants using the ligninolytic enzymes of the white rot fungi has been thoroughly researched and has been shown to have considerable potential for industrial application. However, little success in scale-up and industrialisation of this technology has been attained due to problems associated with the continuous production of the pollutant-degrading enzymes using conventional bioreactor systems. The low productivities reported result from the incompatibility of conventional submerged culture reactor techniques with the physiological requirements of these fungi which have evolved on a solid-air interface, viz. wood. The enzymes are also produced only during the stationary phase of growth and can therefore be regarded as secondary metabolites. This study reports the conceptualisation, characterisation and evaluation of a novel bioreactor system as a solution to the continuous production of idiophasic pollutant degrading enzymes by the white rot fungus Phanerochaete chlysosporium. The reactor concept evolved from observation of these fungi in their native state, i. e. the metabolism of lignocellulosic material and involves the immobilisation of the organism onto a capillary ultrafiltration membrane. Nutrient gradients established across the biofilm, an inherent characteristic of fixed bed perfusion reactors, are exploited to provide both nutrient rich and nutrient poor zones across the biofilm. This allows growth or primary metabolism in the nutrient rich zone, pushing older biomass into the nutrient poor zone where secondary metabolism is induced by nutrient starvation. In effect, this represents a transformation of the events of a batch culture from a temporal to a spatial domain, allowing continuous production of secondary metabolites over time. Direct contact of the outer part of the biofilm with an air stream simulated the solid-air interface of the native state of the fungus. In order to facilitate the practical application of the membrane gradostat reactor (MGR) concept, conventional capillary membranes and membrane bioreactor modules were first evaluated. These were found to be unsuitable for application of the MGR concept. However, critical analysis of the shortcomings of the conventional systems resulted in the formulation of a set of design criteria for the development of a suitable membrane and module. These design criteria were satisfied by the development of a novel capillary membrane for membrane bioreactors, as well as a transverse flow membrane module, which is a novel approach in membrane bioreactor configuration. For the physiological characterisation of the MGR concept, a single fibre bioreactor unit was designed, which allowed destructive sampling of the biofilm for analysis. Using this system, it was shown that distinct morphological zones could be observed radially across the mature biofilm obtained through MGR operation. That these morphotypes do represent the temporal events of a typical batch culture in a spatial domain was confirmed by following the morphological changes occurring during batch culture of the immobilised fungus where the onset of primary and secondary metabolic conditions were manipulated through control of the nutrient supply. The different morphotypes were correlated to distinct growth phases by comparison of the morphology to the secretion of known enzymatic markers for secondary metabolism, viz. succinate dehydrogenase and cytochrome C oxidoreductase. Detailed structure-function analysis of the biofilm using transmission electron microscopy and adapted enzyme cytochemical staining techniques showed that the biofilm appeared to operate as a co-ordinated unit, with primary and secondary metabolism apparently linked in one thallus through nutrient translocation. This study provided new insights into the physiology of P. chrysosp,o rium and a detailed descriptive model was formulated which correlates well to existing models of wood degradation by the white rot fungi (WRF). Evaluation of the process on a laboratory scale using a novel transverse flow membrane bioreactor showed that a volumetric productivity of 1916 U.L.⁻¹day⁻¹ for manganese peroxidase, one of the pollutant degrading enzymes, could be attained, corresponding to a final concentration of 2 361 U.L.⁻¹ This may be compared to the best reported system (Moreira el at. 1997), where a volumetric productivity of 202 U.L.⁻¹day⁻¹was achieved with a final concentration of 250 U.L.⁻¹ However, MGR productivity is yet to be subjected to rigorous optimisation studies. The process could be operated continuously for 60 days. However, peak productivity could not be maintained for long periods. This was found to be due to physical phenomena relating to the fluid dynamics of the system which caused fluid flow maldistribution, which would have to be resolved through engineering analysis. In evaluation of the MGR concept for aromatic pollutant removal, in this case ρ- cresol, from growth medium, good performance was also achieved. The VmaxKm calculated by linear regression for the MGR was 0.8 (R² = 0.93), which compared favourably to that reported by Lewandowski et al. (1990), who obtained a Vmax/Km of 0.34 for a packed bed reactor treating chlorophenol. It was concluded that the MGR showed suitable potential to warrant further development, and that the descriptive characterisation of the biofilm physiology provided a sufficient basis for process analysis once engineering aspects ofthe system could be resolved.
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Machado, Suellen Emilliany Feitosa. "Produção de proteases por fungos isolados no semiárido da Paraíba e na Antártida." Universidade Estadual da Paraíba, 2015. http://tede.bc.uepb.edu.br/jspui/handle/tede/3008.

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Proteases are essential constituents of all living beings, since they are involved in essential biological processes such as blood clotting, cell death, tissue differentiation, protein transport across the membrane etc. They also have important biotechnological applicability, because they can be used in food processing, manufacture of detergents, leather processing, meat softening, drug formulation, in the textile industry etc. These enzymes represents about 60% of the global market for industrial enzymes; so, they are considered an important group of enzymes. This work was divided into two stages and aimed to isolate filamentous fungi collected from coconut trees and soil from a coconut located in Varzeas de Sousa, Paraiba, Brazil and do a screening for the production of proteases and to evaluate, in a bioreactor, the production of proteases by the yeast Rhodotorula mucilaginosa L07. In all, 32 fungi were isolated in Paraiba semiarid. They were grown in rotary shaker and sent to the analysis of proteolytic activity. The specie, originally called Fung1, showed better results in the qualitative stage and was taken to the molecular identification and selected for production in rotary shaker (30°C / 200rpm / 240h). R. mucilaginosa L07, originally from Antarctica, was cultivated in a bioreactor (25°C / 72h), varying agitation and aeration. The maximum enzyme activity by the Fung1, identified as Aspergillus tubingensis, was 29 U.mL^-1, after 144h cultivation. This fungus is not a fumonisin B2 and ochratoxin A producer. The greatest value of proteolytic activity of R. mucilaginosa L07 was 124.88 U.mL^-1 with agitation of 500rpm and aeration 1,0vvm. The results indicated that A. tubingensis produces proteases, but other studies are needed to optimize production and classify proteases. The supply of oxygen to R. mucilaginosa L07 were positive for proteolytic activity, because it increased from 33.36 to 124.88 U.mL^-1 in rotary shaker and bioreactor, respectively.
Proteases são constituintes essenciais em todos os seres vivos, pois estão envolvidas em processos biológicos essenciais como coagulação sanguínea, morte celular, diferenciação de tecidos, transporte de proteínas através da membrana etc. Também possuem importante aplicabilidade biotecnológica, pois podem ser usadas no processamento de alimentos, formulação de detergentes, processamento de couro, amaciamento de carnes, formulação de medicamentos, na indústria têxtil etc. Por representarem aproximadamente 60% do mercado mundial de enzimas industriais, são consideradas um importante grupo de enzimas. Este trabalho foi dividido em duas etapas e objetivou isolar fungos filamentosos coletados em coqueiros e solo de um coqueiral localizado nas Várzeas de Sousa, Paraíba, Brasil e fazer um screening quanto à produção de proteases, além de avaliar, em biorreator, a produção de proteases pela levedura Rhodotorula mucilaginosa L07. Ao todo, 32 fungos foram isolados no semiárido paraibano, cultivados em agitador rotatório e encaminhados à análise da atividade proteolítica. A espécie inicialmente denominada Fung1 apresentou melhor resultado na etapa qualitativa, foi encaminhada à identificação molecular e selecionada para a produção em agitador rotatório (30°C/ 200rpm/ 240h). A R. mucilaginosa L07, coletadada na Antártida, foi cultivada em biorreator (25°C/ 72h), variando agitação e aeração. A atividade enzimática máxima do Fung1, identificado como Aspergillus tubingensis, foi 29 U.mL , após 144h de cultivo. Este fungo não é produtor de fumonisina B2 e ocratoxina A. O maior valor de atividade proteolítica da R. mucilaginosa L07 foi de 124,88 U.mL^-1 , com agitação de 500rpm e aeração de 1,0vvm. Os resultados indicaram que A. tubingensis produz proteases, porém outros estudos são necessários para otimizar a produção e classificar as proteases. O fornecimento de oxigênio em cultivos da R. mucilaginosa L07 foi positivo para a atividade proteolítica, pois a mesma aumentou de 33,36 para 124,88 U.mL^-1, em agitador rotatório e biorreator, respectivamente.
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Cunha, Lucas Portilho da. "Aspectos de engenharia da produção do fungo entomopatogênico Metarhizium anisopliae em biorreator de bandeja /." São José do Rio Preto, 2016. http://hdl.handle.net/11449/138192.

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Orientador: João Cláudio Thoméo
Coorientador: Fernanda Perpétua Casciatori
Banca: Vanildo Luiz Del Bianchi
Banca: Fabio Bentes Freire
Resumo: O presente trabalho teve como objetivo principal o desenvolvimento de um biorreator de bandeja capaz de produzir esporos do fungo entomopatogênico Metarhizium anisopliae. Para esse fim, foi utilizado um biorreator de bandeja para a produção de esporos, que por sua vez foi avaliada em função da carga de substrato empregada. As variações de temperatura no biorreator foram simuladas e observadas experimentalmente. Para o desenvolvimento do biorreator, inicialmente foram realizados ensaios em embalagens plásticas contendo 10g e 500g de substrato, de modo a avaliar como o aumento da carga de meio fermentativo poderia influenciar na produção de esporos. Nestes ensaios, foram medidas as temperaturas no centro geométrico das embalagens e a elevação de temperatura foi relacionada à produção de esporos. Realizou - se uma análise de área superficial do arroz tipo 1 e da quirera de arroz para avaliar a interferência desta propriedade na produção de esporos. A partir dos resultados em embalagens plásticas foram realizados experimentos em um biorreator de 40 cm de comprimento, 29 cm de largura por 12 cm de altura, escoando ar sobre as partículas no sentido da maior dimensão. Foram empregadas cargas de 1, 2 e 3kg de arroz tipo 1, correspondente à altura de leito de 2, 4 e 6 cm, respectivamente, e 1 kg de quirera, correspondendo a 2 cm de altura. A temperatura nas posições de entrada e saída de ar e no meio geométrico do meio de cultivo foram medidas ao longo dos ensaios. A fermentação nas embalagens plásticas e no biorreator foram realizadas a 28oC. Para estimar a geração de calor metabólico do meio de cultivo, foram coletados dados de consumo de O2 e liberação de CO2 durante o período de incubação. Para tal, foi realizada a fermentação em um biorreator de leito empacotado cilíndrico, sendo os gases provenientes do leito e conduzidos a um analisador de...
Abstract: This study aimed to develop a bioreactor tray capable of producing spores of the fungus Metarhizium anisopliae entomopathogenic. To this end, we used a tray bioreactor for the production of spores, which in turn was evaluated according to the employed substrate load. Temperature variations in the bioreactor were simulated and observed experimentally. For the development of bioreactor assays were carried out in plastic bags containing substrate 10g and 500g in order to evaluate how increasing fermentative medium loading could influence the production of spores. In these tests we measured the temperature at the geometric center of the packages and the temperature rise was related to spore production performed -. A surface area analysis of rice Type 1 and broken rice to evaluate the interference at this property in the production of spores. From the results in plastic containers experiments were performed in a bioreactor of 40 cm long, 29 cm wide by 12 cm, air flowing onto the particles in the direction of the largest dimension. Loads were applied to 1, 2 and 3 kg of rice type 1, corresponding to the bed height of 2, 4 and 6 cm, respectively, and 1 kg of grits, corresponding to 2 cm. The temperature at the inlet position and the air outlet and the geometric mean of the culture medium were measured throughout the tests. Fermentation in plastic packaging and the bioreactor were carried out at 28 C. To estimate the generation of metabolic heat of culture medium were collected consumption data The O2 and CO2 release during the incubation period. To this end, the fermentation was performed in a bioreactor cylindrical packed bed, and the gases from the bed and driven to a gas analyzer. A onedimensional mathematical model capable of predicting the temperature profile and fungal growth during the process at any position of the bioreactor is proposed. The experimental results in plastic ...
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Cunha, Lucas Portilho da. "Produção de esporos do fungo Metarhizium anisopliae IBCB 425 utilizando biorreator de leito empacotado /." São José do Rio Preto, 2020. http://hdl.handle.net/11449/192754.

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Orientador: João Cláudio Thoméo
Resumo: Este trabalho tem como objetivo produzir esporos do fungo Metarhizium anisopliae IBCB 425 em biorreator de leito empacotado visando o aumento da escala, propondo ainda alternativas para a extração dos esporos do meio de cultivo. Também se propõe compreender a preferência do fungo pelo arroz como substrato, procurando-se alternativas para redução dos custos de produção relativos a este substrato. Para isso, realizou-se o cultivo e o reuso do substrato em embalagens plásticas, onde se observou que o cultivo em arroz tipo 1 ainda é o melhor substrato para o fungo, quando comparado com os utilizados neste trabalho sendo, quirera de arroz e farelo de arroz, porém para o aumento de escala e reuso do substrato, a melhor alternativa é a mistura de arroz com bagaço de cana-de-açúcar na proporção de 9:1. Também se realizou ensaios em biorreatores de leito empacotado de 7.62 cm e 20 cm de diâmetro, utilizando a mistura de arroz e bagaço como substrato, e observou-se que, para ambas as configurações, o bagaço evitou a compactação do leito, não se observando temperaturas elevadas que interferissem no desenvolvimento do microrganismo. Com as análises de degradação do amido e da atividade das enzimas amilase total, alfa – amilase e protease, foi possível concluir que o fungo se desenvolve de maneira distinta a cada uso dos grãos e que, o maior consumo de amido ocorre no primeiro cultivo (R1), reduzindo-o em cerca de 30%. Análises de microscopia óptica foram realizadas nos grãos cultivados, ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: This work aimed to produce spores of the entomopathogenic fungus Metarhizium anisopliae IBCB 425 in packed-bed bioreactors targeting the scale-up, and also to propose alternatives to the extraction of the spores from the cultivation medium. The understanding of the preference of the fungus for the long rice as substrate was also verified in order to reduce the production costs associated to the rice. Therefore, the rice was cultivated in plastic packages and it was noticed that rice is still the best substrate for the fungal growth, even though for the scale-up and reutilization of the substrate it was necessary to mix it with sugarcane bagasse at a proportion 9:1 (rice:bagasse). Experiments in packed-beds of 7.62 and 20 cm internal diameter have been carried out using the mixture rice:bagasse and for both sizes no compaction was noted and no excessive temperature took place. From starch analysis and from the activities of total amylase, alpha-amylase and protease it was noticed that the microbe develops distinctly in each consecutive cultivation of rice, and that after the first cultivation (R1), the starch content was reduced by 30%. Optical microscopy of the cultivated grains revealed that during R1 the fungal hyphaes penetrated the grains, but during the second (r2) and third (R3) cultivations the fungal growth is restricted to the grain surface. It was observed a substrate mass reduction in the packed-bed experiments of 24%, 20% and 17% after R1, R2 and R3, respectively.... (Complete abstract click electronic access below)
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Book chapters on the topic "Fungal bioreactors"

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Karpe, Avinash V., Rohan M. Shah, Vijay Dhamale, Snehal Jadhav, David J. Beale, and Enzo A. Palombo. "Bioreactors and other Technologies used in Fungal Bioprocesses." In Fungal Bioremediation, 287–353. Boca Raton, FL: CRC Press, 2019. | “A science publishers book.”: CRC Press, 2019. http://dx.doi.org/10.1201/9781315205984-11.

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De Araujo, A. A., C. Lepilleur, S. Delcourt, P. Colavitti, and S. Roussos. "Laboratory scale bioreactors for study of fungal physiology and metabolism in solid state fermentation system." In Advances in Solid State Fermentation, 93–111. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-017-0661-2_8.

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Tabatabaei, Meisam, Amin Alidadi, Mona Dehhaghi, Hamed Kazemi Shariat Panahi, Su Shiung Lam, Abdul-Sattar Nizami, Mortaza Aghbashlo, and Gholamreza Salehi Jouzani. "Fungi as Bioreactors for Biodiesel Production." In Fungi in Fuel Biotechnology, 39–67. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-44488-4_3.

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Qin, Hao, Jun-Wei Xu, Jian-Hui Xiao, Ya-Jie Tang, Han Xiao, and Jian-Jiang Zhong. "Cell Factories of Higher Fungi for Useful Metabolite Production." In Bioreactor Engineering Research and Industrial Applications I, 199–235. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/10_2015_335.

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Espinosa-Ortiz, Erika Jimena. "Removal of selenite from wastewater in a Phanerochaete chrysosporium pellet based fungal bioreactor." In Bioreduction of selenite and tellurite by Phanerochaete chrysosporium, 66–85. London: CRC Press, 2021. http://dx.doi.org/10.1201/9781315138381-10.

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Yan, Jing-Kun, and Jian-Yong Wu. "Submerged Fermentation of Medicinal Fungus Cordyceps sinensis for Production of Biologically Active Mycelial Biomass and Exopolysaccharides." In Production of Biomass and Bioactive Compounds Using Bioreactor Technology, 93–120. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9223-3_5.

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Singhal, Uma, Manpreet Kaur Attri, and Ajit Varma. "Mass Cultivation of Mycorrhiza-Like Fungus Piriformospora indica (Serendipita indica) by Batch in Bioreactor." In Mycorrhiza - Function, Diversity, State of the Art, 365–84. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-53064-2_18.

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"Bacterial, Yeast, and Fungal Cultures." In Bioreactor System Design, 67–108. CRC Press, 1994. http://dx.doi.org/10.1201/9781482277470-7.

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Singh, Gurmeet. "Application of Fungal Elicitation for Enhancing Production of Secondary Metabolites by Hairy Roots Cultured in Large-Scale Bioreactors." In Hairy Roots, 209–18. CRC Press, 2020. http://dx.doi.org/10.1201/9780367810610-19.

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Berovic, Marin, and Bojana Boh Podgornik. "Cultivation of Medicinal Fungi in Bioreactors." In Mushroom Biotechnology, 155–71. Elsevier, 2016. http://dx.doi.org/10.1016/b978-0-12-802794-3.00009-6.

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Conference papers on the topic "Fungal bioreactors"

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SINGH, S. S., and A. K. DIKSHIT. "BACTERIAL AND FUNGAL BIOREACTORS FOR COLOUR REMOVAL FROM DISTILLERY SPENTWASH." In Proceedings of the International Conference on CBEE 2009. WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789814295048_0012.

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Iram, Attia, Deniz Cekmecelioglu, and Ali Demirci. "The effect of Dilution, Aeration, and Agitation on Fungal Cellulase and Xylanase Production by DDGS-based Fermentation Media in Stirred Tank Bioreactors." In 2022 Houston, Texas July 17-20, 2022. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2022. http://dx.doi.org/10.13031/aim.202200147.

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BINGJUN HE and WEI WEN SU. "Mixing Estimation of a Laboratory-Scale External-loop Air-lift Bioreactor for Fungal Culture Using pH Tracer Method." In 2003, Las Vegas, NV July 27-30, 2003. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2003. http://dx.doi.org/10.13031/2013.14240.

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Hiroko Isoda, Keo Intabon, Norio Sugiura, and Takaaki Maekawa. "Development of Functional Foodstaffs Operated by a Liquid Bioreactor of an Edible Fungus." In 2001 Sacramento, CA July 29-August 1,2001. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2001. http://dx.doi.org/10.13031/2013.3674.

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Cantero, Katty M., and Marco E. Sanjuan. "Dynamic Model Fuzzy Transition in DMC Type Controllers for Varying Dynamics in Bioreactors." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-67471.

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This research presents an approach to implement industrial type DMC (Dynamic Matrix Control) controllers in biological process where a fed-batch operation is required. In this type of operation a substantial change in the process dynamics occurs due to changes in volume, reaction rate, pressure, temperature, concentration, and other process variables. To compensate for changes in the dynamic, several matrix models are identified, and using a fuzzy supervisor a soft model transition is implemented, such that the controller and the process performance can adapt to the new operating conditions without affecting the process integrity. The control system design, its identification, and implementation are presented, focusing in the articulation between MPC (Model Predictive Control) and Fuzzy Supervision. The overall strategy is demonstrated at a simulation level using a benchmark fermentation process for Penicillin production in Fed-Batch mode. In this process glucose is used as a substrate, and a fungus, penicillium chrysogenum, is used in a fed-batch reactor to obtain penicillin. The model presents an unstructured approach to the reactor dynamic system, and the initial steps for this research required the development of an identification procedure for such zones.
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Chirnside, Anastasia E. M. "Effect of Spent Mushroom Compost Nutrient Levels on the Efficiency of a Fungal Bioreactor to Remove Escherichia Coli from Aqueous Manure Waste Streams." In World Environmental and Water Resources Congress 2016. Reston, VA: American Society of Civil Engineers, 2016. http://dx.doi.org/10.1061/9780784479889.042.

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Keo INTABON, Takaaki MAEKAWA, and Norio SUGIURA. "A Scale Up and Operation Problems for a Liquid Bioreactor of an Edible Fungus (Mushroom Agaricus blazei Murill.)." In 2001 Sacramento, CA July 29-August 1,2001. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2001. http://dx.doi.org/10.13031/2013.4057.

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