Relevant bibliographies by topics / Functional systems analysis

Academic literature on the topic 'Functional systems analysis'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Functional systems analysis"

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Schoenemann, Brigitte, and Euan N. K. Clarkson. "At first sight Functional Analysis of Lower Cambrian Eye Systems." Palaeontographica Abteilung A 297, no. 5-6 (November 19, 2012): 123–49. http://dx.doi.org/10.1127/pala/297/2012/123.

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Ferreira, José M., and Sandra Pinelas. "Oscillatory retarded functional systems." Journal of Mathematical Analysis and Applications 285, no. 2 (September 2003): 506–27. http://dx.doi.org/10.1016/s0022-247x(03)00421-9.

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Fantoni, G., Arnaud De Grave, and Hans Norgaard Hansen. "Functional analysis for micro mechanical systems." International Journal of Design Engineering 3, no. 4 (2010): 355. http://dx.doi.org/10.1504/ijde.2010.040522.

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Singh, R. K., P. Singh, and V. K. Pandey. "Functional homomorphisms and dynamical systems." Banach Journal of Mathematical Analysis 4, no. 2 (2010): 37–44. http://dx.doi.org/10.15352/bjma/1297117239.

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Röckner, Michael, and Feng-Yu Wang. "Functional Inequalities for Particle Systems on Polish Spaces." Potential Analysis 24, no. 3 (May 2006): 223–43. http://dx.doi.org/10.1007/s11118-005-0913-6.

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Pietrucha-Urbanik, K., and B. Tchórzewska-Cieślak. "Water network functional analysis." IOP Conference Series: Earth and Environmental Science 900, no. 1 (November 1, 2021): 012034. http://dx.doi.org/10.1088/1755-1315/900/1/012034.

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Abstract Water distribution systems should have a high level of reliability and availability. Water distribution system failures should be diagnosed and categorised, according to their consequences, causes, frequency, and other important factors. A failure analysis of the water distribution system is considered in this study, as well as a method for establishing a failure susceptibility index and evaluating the risk of failures within a defined area, based on categories and zonal characteristics. A risk scale, such as tolerable, controlled, and unacceptable, will be used to assess the risk of failure. The methodology is provided to help in the performance and risk assessments of water distribution systems, as well as decision-making.
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Thompson, Travis. "RELATIONS AMONG FUNCTIONAL SYSTEMS IN BEHAVIOR ANALYSIS." Journal of the Experimental Analysis of Behavior 87, no. 3 (May 2007): 423–40. http://dx.doi.org/10.1901/jeab.2007.21-06.

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Viveros, Pablo, René Tapia, Fredy Kristjanpoller, Luis Barberá, and Vicente González-Prida. "FUNCTIONAL STOCKPILE ANALYSIS IN COMPLEX PRODUCTIVE SYSTEMS." IFAC Proceedings Volumes 45, no. 31 (2012): 13–18. http://dx.doi.org/10.3182/20121122-2-es-4026.00008.

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Khodaygan, S., and M. R. Movahhedy. "Functional process capability analysis in mechanical systems." International Journal of Advanced Manufacturing Technology 73, no. 5-8 (May 9, 2014): 899–912. http://dx.doi.org/10.1007/s00170-014-5800-0.

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Kuzmenko, Alexander. "Functional analysis of popular content management systems." Ergodesign, no. 4 (November 18, 2022): 262–66. http://dx.doi.org/10.30987/2658-4026-2022-4-262-266.

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The article discusses various functions of the web content management system that must be taken into account when developing an educational website. A number of improvements are presented for the development of CMS-based web applications based on an approach based on the lifecycle model of content management systems. These materials are recommendations that allow you to improve the overall quality of the CMS being developed.
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Dissertations / Theses on the topic "Functional systems analysis"

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Schmelich, Volker, and Rainer Alt. "Functional Analysis of Open Source ERP Systems – An Exploratory Analysis." Universitätsbibliothek Leipzig, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-81833.

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Während Open Source-Software wie etwa Linux, der Apace Webserver oder die OpenOffice-Suite eine grosse Verbreitung erfahren haben, sind Open Source-Lösungen zur Unterstützung betrieblicher Aufgaben weniger bekannt. Dieser Bericht unternimmt ausgehend von den funktionalen Anforderungen der Auftragsabwicklung eines Industrieunternehmens einen Vergleich bestehender Lösungen und diskutiert die Einsatzmöglichkeiten aus heutiger Sicht.
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Schmelich, Volker, and Rainer Alt. "Functional Analysis of Open Source ERP Systems – An Exploratory Analysis." Universität Leipzig, 2008. https://ul.qucosa.de/id/qucosa%3A11328.

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Während Open Source-Software wie etwa Linux, der Apace Webserver oder die OpenOffice-Suite eine grosse Verbreitung erfahren haben, sind Open Source-Lösungen zur Unterstützung betrieblicher Aufgaben weniger bekannt. Dieser Bericht unternimmt ausgehend von den funktionalen Anforderungen der Auftragsabwicklung eines Industrieunternehmens einen Vergleich bestehender Lösungen und diskutiert die Einsatzmöglichkeiten aus heutiger Sicht.:1 Introduction 2 Research Methodology 3 Functional Analysis of OS-ERP 3.1 Setting: A Manufacturing Company 3.2 Order Processing as Scenario for Comparison 3.3 Results of Comparison 3.4 Discussion of Results 4 Conclusions
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MacDonell, Stephen Gerard. "Quantitative functional complexity analysis of commercial software systems." Thesis, University of Cambridge, 1993. https://www.repository.cam.ac.uk/handle/1810/270422.

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Tan, Sofia. "Enhanced functional analysis system technique for managing complex engineering projects." Diss., Rolla, Mo. : University of Missouri-Rolla, 2007. http://scholarsmine.umr.edu/thesis/pdf/Tan_09007dcc803c4e70.pdf.

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Thesis (M.S.)--University of Missouri--Rolla, 2007.
Vita. The entire thesis text is included in file. Title from title screen of thesis/dissertation PDF file (viewed November 27, 2007) Includes bibliographical references (p. 27-29).
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Adams, Anne Edith. "Understanding the skill of functional task analysis." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/37313.

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Although widely used, little is known about the nature of expertise involved in functional task analysis, methods used to discover and represent a task structure in terms of goals and subgoals. Training studies indicated that learning task analysis is not trivial. To counter the "task analysis is an art" explanation, this dissertation approached task analysis as a skill acquisition problem that can be understood through scientific inquiry. Two studies were designed to capture and characterize experienced and novice performance. Professional (Study 1) and novice (Study 2) task analysts conducted task analyses on six tasks from two domains (cooking, communication). Master task analyses were created for each task and served as a basis for analysis. Some similar patterns to the task analysis products and errors were observed for the hierarchy dimensions (breadth and depth of analysis), subgoal focus, and versatility. However, differences in separating subgoals (verb-noun pairs) were observed and may be further investigated in the future. Future directions could also focus on understanding the association between the general approach (breadth and depth-first) and the characteristics of the task analysis products. Skill components of functional task analysis were derived from the findings in both studies conducted for this dissertation.
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Nayfeh, Jamal Faris. "Nonlinear dynamics of composite plates and other physical systems." Diss., This resource online, 1990. http://scholar.lib.vt.edu/theses/available/etd-07282008-135756/.

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Xu, Yiwen. "Reliability Analysis and Optimization of Systems Containing Multi-Functional Entities." Diss., The University of Arizona, 2015. http://hdl.handle.net/10150/594927.

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Enabling more than one function in an entity provides a new cost-effective way to develop a highly reliable system. In this dissertation, we study the reliability of systems containing multi-functional entities. We derive the expressions for reliability of one-shot systems and reliability of each function. A step further, a redundancy allocation problem (RAP) with the objective of maximizing system reliability is formulated. Unlike constructing a system with only single-functional entities, the number of copies of a specific function to be included in each multi-functional entity (i.e., functional redundancy) needs to be determined as part of the design. Moreover, a start-up strategy for turning on specific functions in these components must be decided prior to system operation. We develop a heuristic algorithm and include it in a two-stage Genetic Algorithm (GA) to solve the new RAP. We also apply a modified Tabu search (TS) method for solving such NP-hard problems. Our numerical studies illustrate that the two-stage GA and the TS method are quite effective in searching for high quality solutions. The concept of multi-functional entities can be also applied in probabilistic site selection problem (PSSP). Unlike traditional PSSP with failures either at nodes or on edges, we consider a more general problem, in which both nodes and edges could fail and the edge-level redundancy is included. We formulate the problem as an integer programming optimization problem. To reduce the searching space, two corresponding simplified models formulated as integer linear programming problems are solved for providing a lower bound to the primal problem. Finally, a big challenge in reliability analysis is how to determine the failure distribution of components. This is especially significant for multi-functional entities as more levels of redundancy are considered. We provide an automated model-selection method to construct the best phase-type (PH) distribution for a given data set in terms of the model complexity and the adequacy of statistical fitting. To efficiently utilize the Akaike Information Criterion for balancing the likelihood value and the number of free parameters, the proposed method is carried out in two stages. The detailed subproblems and the related solution procedures are developed and illustrated through numerical studies. The results verify the effectiveness of the proposed model-selection method in constructing PH distributions.
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Cole, David Michael. "Functional network analysis of human brain systems under pharmacological modulation." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/10933.

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Complex alterations in brain function and neurochemistry underlie pathology and treatment in multiple neuropsychiatric disorders, yet remain incompletely characterised. This thesis outlines possibilities for human neuroimaging techniques sensitive to spontaneous fluctuations in large-scale neurobiological signalling, or ‘resting-state network (RSN) functional connectivity’, to address such knowledge gaps. Novel RSN-sensitive analysis approaches to functional magnetic resonance imaging data are introduced. These techniques are then evaluated experimentally, in contexts relevant for maladaptive cognitive and motivational processing, for their utility to identify and characterise systems-level signatures of individual differences in neurochemistry and psychopharmacological responsiveness. Firstly, RSN functional connectivity measures are investigated in the context of pharmacological intervention with nicotine replacement therapy in habitual smokers. Results identify connectivity between executive control and ‘default mode’ RSNs as a neural signature of pharmacotherapeutic efficacy in treating cognitive symptoms of nicotine withdrawal. Secondly, RSN connectivity is investigated alongside specific neuroreceptor-sensitive measures to investigate the extent to which network connectivity patterns reflect fundamental neurobiology in healthy subjects. Individual differences in dopamine D3 receptor availability - a possible marker for reward-related behaviours -are associated with topographic connectivity signatures within RSNs implicated in cognitive and motivational control. Thirdly, the ability of RSN metrics to characterise distinct neurochemical manipulations is tested in healthy subjects. Dopamine agnostic and antagonistic neuromodulations display differential effects on signalling in cortico-cubcortical and cortico-cortical reward circuitry and interact selectively with subject impulsivity. Finally, RSN cortico-subcortical connectivity metrics are investigated for their sensitivity to clinical-pharmacological effects in Parkinson’s disease. Results reveal evidence for both compensatory large-scale network mechanisms and ‘non-normalising’ dopaminergic medication effects in patients. Overall, findings indicate novel systems-level neuroimaging methodology probing interactions within and between RSNs to provide sensitive, biologically plausible markers for behavioural and neuropharamacological characteristics of neuropsychiatric disorders. Continued developments of functional network analysis approaches may facilitate their direct application to clinical and drug development domains.
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Bell, Jonathan. "Interpretation of simulation for model-based design analysis of engineered systems." Thesis, Aberystwyth University, 2006. http://hdl.handle.net/2160/05880836-32b8-4f65-abfe-9b0ecf16cc42.

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This thesis attempts to answer the question "Can we devise a language for interpretation of behavioural simulation of engineered systems (of arbitrary complexity) in terms of the systems’ purpose?" It does so by presenting a language that represents a device’s function as achieving some purpose if the device is in a state that is intended to trigger the function and the function’s expected effect is present. While most work in the qualitative and model-based reasoning community has been concerned with simulation, this language is presented as a basis for interpret- ing the results of the simulation of a system, enabling these results to be expressed in terms of the system’s purpose. This, in turn, enables the automatic production of draft design analysis reports using model based analysis of the subject system. The increasing behavioural complexity of modern systems (resulting from the increasing use of microprocessors and software) has led to a need to interpret the results of simulation in cases beyond the capabilities of earlier functional mod- elling languages. The present work is concerned with such cases and presents a functional modelling language that enables these complex systems to be analysed. Specifically, the language presented herein allows functional description and interpretation of the following. • Cases where it is desired to distinguish between partial and complete failure of a function. • Systems whose functionality depends on achieving a sequence of intermittent effects. • Cases where a function being achieved in an untimely manner (typically late) needs to be distinguished from a function failing completely. • Systems with functions (such as warning functions) that depend upon the state of some other system function. This offers significant increases both in the range of systems and of design analysis tasks for which the language can be used, compared to earlier work.
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Chang, Ken Kai-fu 1973. "Simulation of nonlinear optic-fibre communication systems using Volterra series transfer function techniques." Monash University, Dept. of Electrical and Computer Systems Engineering, 2002. http://arrow.monash.edu.au/hdl/1959.1/7758.

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Books on the topic "Functional systems analysis"

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Baylin, Edward N. Functional modeling of systems. New York: Gordon and Breach Science Publishers, 1990.

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V, Sudakov K., Ganten D. 1941-, Nikolov Nikola A, and International Seminar on the Development of the General Theory of Functional Systems (1982 : Suzdalʹ, R.S.F.S.R.), eds. Motivation in functional systems. New York: Gordon and Breach, 1987.

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Functional analysis: Simplify before automating. New York: Van Nostrand Reinhold, 1991.

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Functional analysis and control theory: Linear systems. Dordrecht: D. Reidel, 1987.

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Belitskii, Genrich. One-dimensional Functional Equations. Basel: Birkhäuser Basel, 2003.

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Gilʹ, M. I. Explicit stability conditions for continuous systems: A functional analytic approach. Berlin: Springer, 2005.

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Kim, A. V. Systems with delays: Analysis, control, and computations. Hoboken, New Jersey: John Wiley & Sons, Inc., 2015.

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Quantum mechanics of non-Hamiltonian and dissipative systems. Amsterdam: Elsevier, 2008.

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de, Wouw Nathan van, and Nijmeijer H. 1955-, eds. Uniform output regulation of nonlinear systems: A convergent dynamics approach. Boston: Birkhäuser, 2006.

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Functional analysis: An elementary introduction. Providence, Rhode Island: American Mathematical Society, 2014.

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Book chapters on the topic "Functional systems analysis"

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Wang, Jiguang. "Functional Enrichment Analysis." In Encyclopedia of Systems Biology, 772. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_491.

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Brusa, Eugenio, Ambra Calà, and Davide Ferretto. "Functional Analysis." In Systems Engineering and Its Application to Industrial Product Development, 147–91. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-71837-8_6.

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Rolewicz, Stefan. "Systems with distributed parameters." In Functional Analysis and Control Theory, 328–417. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-015-7758-8_7.

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Temam, Roger. "Elements of Functional Analysis." In Infinite-Dimensional Dynamical Systems in Mechanics and Physics, 43–81. New York, NY: Springer New York, 1997. http://dx.doi.org/10.1007/978-1-4612-0645-3_3.

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Temam, Roger. "Elements of Functional Analysis." In Infinite-Dimensional Dynamical Systems in Mechanics and Physics, 41–79. New York, NY: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-0313-8_3.

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Gupta, Ved Prakash, Prabha Mandayam, and V. S. Sunder. "Operator Systems." In The Functional Analysis of Quantum Information Theory, 63–92. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-16718-3_3.

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Fattorini, Hector O. "Linear Control Systems in Sequence Spaces." In Functional Analysis and Evolution Equations, 273–90. Basel: Birkhäuser Basel, 2007. http://dx.doi.org/10.1007/978-3-7643-7794-6_18.

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Rolewicz, Stefan. "Optimization and observability of linear systems." In Functional Analysis and Control Theory, 165–261. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-015-7758-8_5.

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Rolewicz, Stefan. "Systems governed by ordinary differential equations." In Functional Analysis and Control Theory, 262–327. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-015-7758-8_6.

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Feng, Dexing. "New Progress on Operator Semigroups and Linear Systems in Abstract Spaces." In Functional Analysis in China, 35–52. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-0185-8_4.

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Conference papers on the topic "Functional systems analysis"

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Arlitt, Ryan M., Kristen Donovan Balinski, Cihan H. Dagli, and Katie Grantham. "Functional analysis of systems using a Functional Basis." In 2011 IEEE International Systems Conference (SysCon). IEEE, 2011. http://dx.doi.org/10.1109/syscon.2011.5929052.

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Zhumatov, Sailaubay S. "Instability of the control systems with non-stationary nonlinearities." In INTERNATIONAL CONFERENCE “FUNCTIONAL ANALYSIS IN INTERDISCIPLINARY APPLICATIONS” (FAIA2017). Author(s), 2017. http://dx.doi.org/10.1063/1.5000661.

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Krishnamachari, Ramprasad. "Reconfiguring functional decomposition of systems." In 7th AIAA/USAF/NASA/ISSMO Symposium on Multidisciplinary Analysis and Optimization. Reston, Virigina: American Institute of Aeronautics and Astronautics, 1998. http://dx.doi.org/10.2514/6.1998-4922.

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Muscar, Alex, Costin Bădică, Theodore E. Simos, George Psihoyios, Ch Tsitouras, and Zacharias Anastassi. "A Functional Take on Multi-Agent Systems Position Paper." In NUMERICAL ANALYSIS AND APPLIED MATHEMATICS ICNAAM 2011: International Conference on Numerical Analysis and Applied Mathematics. AIP, 2011. http://dx.doi.org/10.1063/1.3636870.

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Wei Dai, Yu Sun, and Xiaoning Qian. "Functional analysis of grasping motion." In 2013 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS 2013). IEEE, 2013. http://dx.doi.org/10.1109/iros.2013.6696856.

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Stoilov, Todor, and Krasimira Stoilova. "Functional analysis of enterprise resource planning systems." In the 9th International Conference. New York, New York, USA: ACM Press, 2008. http://dx.doi.org/10.1145/1500879.1500927.

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Patil, Navendu, Pradeep Mahadevan, and Anindya Chatterjee. "Fatigue Laws via Functional Equations." In ASME 2008 9th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2008. http://dx.doi.org/10.1115/esda2008-59243.

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In routine industrial design, fatigue life estimation is largely based on S-N curves and ad hoc cycle counting algorithms used with Miner’s rule for predicting life under complex loading. However, there are well known deficiencies of the conventional approach. Of the many cumulative damage rules that have been proposed, Manson’s Double Linear Damage Rule (DLDR) has been the most successful. Here we follow up, through comparisons with experimental data from many sources, on a new approach to empirical fatigue life estimation (‘A Constructive Empirical Theory for Metal Fatigue Under Block Cyclic Loading’, Proceedings of the Royal Society A, in press). The basic modeling approach is first described: it depends on enforcing mathematical consistency between predictions of simple empirical models that include indeterminate functional forms, and published fatigue data from handbooks. This consistency is enforced through setting up and (with luck) solving a functional equation with three independent variables and six unknown functions. The model, after eliminating or identifying various parameters, retains three fitted parameters; for the experimental data available, one of these may be set to zero. On comparison against data from several different sources, with two fitted parameters, we find that our model works about as well as the DLDR and much better than Miner’s rule. We finally discuss some ways in which the model might be used, beyond the scope of the DLDR.
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Lukic, Ana S., Miles N. Wernick, Dimitris G. Tzikas, Xu Chen, Aristidis Likas, Nikolas P. Galatsanos, Yongyi Yang, Fuqiang Zhao, and Stephen C. Strother. "Kernel Methods for Functional Neuroimaging Analysis." In 2006 Fortieth Asilomar Conference on Signals, Systems and Computers. IEEE, 2006. http://dx.doi.org/10.1109/acssc.2006.356606.

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Yang, Yongyi. "Session TA2b: Functional imaging and analysis." In 2008 42nd Asilomar Conference on Signals, Systems and Computers. IEEE, 2008. http://dx.doi.org/10.1109/acssc.2008.5074575.

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Wani, M. F. "Failure Analysis of Mechanical Systems Based on Function-Cum-Structure Approach." In ASME 8th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2006. http://dx.doi.org/10.1115/esda2006-95726.

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This paper suggest failure analysis of mechanical systems using function cum structure approach. Operational parameters of system function are identified and their relationships are developed. The predicates, which comprise the components of the system and their properties as their attributes, i.e., type of connectivity (fixed or sliding contact) and functions (transmit torque, and transmit force etc.) represent the facts. The facts are modelled in terms of functional-cum-structure graph. By analysing interaction of a failure function i.e., function of a failed component in the graph with other functions, the failure cause-function is identified. The problem can either be solved by removing the cause-functions or by modifying the failure function. A critical function is also identified from function set on the basis of importance of a function. Appropriate parameters for in-situ design provision for condition monitoring of the system are also identified. The steps of the methodology are included and are illustrated by means of an example.
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Reports on the topic "Functional systems analysis"

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Foster, Rosemary. Functional Analysis of Neurofibromin: Clues from Drosophila Applied to Mammalian Systems. Fort Belvoir, VA: Defense Technical Information Center, November 2001. http://dx.doi.org/10.21236/ada400501.

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Foster, Rosemary. Functional Analysis of Neurofibromin: Clues From Drosophila Applied to Mammalian Systems. Fort Belvoir, VA: Defense Technical Information Center, November 2002. http://dx.doi.org/10.21236/ada412174.

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Ron, Eliora, and Eugene Eugene Nester. Global functional genomics of plant cell transformation by agrobacterium. United States Department of Agriculture, March 2009. http://dx.doi.org/10.32747/2009.7695860.bard.

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The aim of this study was to carry out a global functional genomics analysis of plant cell transformation by Agrobacterium in order to define and characterize the physiology of Agrobacterium in the acidic environment of a wounded plant. We planed to study the proteome and transcriptome of Agrobacterium in response to a change in pH, from 7.2 to 5.5 and identify genes and circuits directly involved in this change. Bacteria-plant interactions involve a large number of global regulatory systems, which are essential for protection against new stressful conditions. The interaction of bacteria with their hosts has been previously studied by genetic-physiological methods. We wanted to make use of the new capabilities to study these interactions on a global scale, using transcription analysis (transcriptomics, microarrays) and proteomics (2D gel electrophoresis and mass spectrometry). The results provided extensive data on the functional genomics under conditions that partially mimic plant infection and – in addition - revealed some surprising and significant data. Thus, we identified the genes whose expression is modulated when Agrobacterium is grown under the acidic conditions found in the rhizosphere (pH 5.5), an essential environmental factor in Agrobacterium – plant interactions essential for induction of the virulence program by plant signal molecules. Among the 45 genes whose expression was significantly elevated, of special interest is the two-component chromosomally encoded system, ChvG/I which is involved in regulating acid inducible genes. A second exciting system under acid and ChvG/Icontrol is a secretion system for proteins, T6SS, encoded by 14 genes which appears to be important for Rhizobium leguminosarum nodule formation and nitrogen fixation and for virulence of Agrobacterium. The proteome analysis revealed that gamma aminobutyric acid (GABA), a metabolite secreted by wounded plants, induces the synthesis of an Agrobacterium lactonase which degrades the quorum sensing signal, N-acyl homoserine lactone (AHL), resulting in attenuation of virulence. In addition, through a transcriptomic analysis of Agrobacterium growing at the pH of the rhizosphere (pH=5.5), we demonstrated that salicylic acid (SA) a well-studied plant signal molecule important in plant defense, attenuates Agrobacterium virulence in two distinct ways - by down regulating the synthesis of the virulence (vir) genes required for the processing and transfer of the T-DNA and by inducing the same lactonase, which in turn degrades the AHL. Thus, GABA and SA with different molecular structures, induce the expression of these same genes. The identification of genes whose expression is modulated by conditions that mimic plant infection, as well as the identification of regulatory molecules that help control the early stages of infection, advance our understanding of this complex bacterial-plant interaction and has immediate potential applications to modify it. We expect that the data generated by our research will be used to develop novel strategies for the control of crown gall disease. Moreover, these results will also provide the basis for future biotechnological approaches that will use genetic manipulations to improve bacterial-plant interactions, leading to more efficient DNA transfer to recalcitrant plants and robust symbiosis. These advances will, in turn, contribute to plant protection by introducing genes for resistance against other bacteria, pests and environmental stress.
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Katzir, Nurit, James Giovannoni, Marla Binzel, Efraim Lewinsohn, Joseph Burger, and Arthur Schaffer. Genomic Approach to the Improvement of Fruit Quality in Melon (Cucumis melo) and Related Cucurbit Crops II: Functional Genomics. United States Department of Agriculture, January 2010. http://dx.doi.org/10.32747/2010.7592123.bard.

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Background: Genomics tools for enhancement of melon research, with an emphasis on fruit, were developed through a previous BARD project of the PIs (IS -333-02). These included the first public melon EST collection, a database to relay this information to the research community and a publicly available microarray. The current project (IS-3877- 06) aimed to apply these tools for identification of important genes for improvement of melon (Cucumis melo) fruit quality. Specifically, the research plans included expression analysis using the microarray and functional analyses of selected genes. The original project objectives, as they appeared in the approved project, were: Objective 1: Utilization of a public melon microarray developed under the existing project to characterize melon transcriptome activity during the ripening of normal melon fruit (cv. Galia) in order to provide a basis for both a general view of melon transcriptome activity during ripening and for comparison with existing transcriptome data of developing tomato and pepper fruit. Objective 2: Utilization of the same public melon microarray to characterize melon transcriptome activity in lines available in the collection of the Israeli group, focusing on sugar, organic acids and aroma metabolism, so as to identify potentially useful candidates for functional analysis and possible manipulation, through comparison with the general fruit development profile resulting from (1) above. Objective 3: Expansion of our existing melon EST database to include publicly available gene expression data and query tools, as the US group has done with tomato. Objective 4: Selection of 6-8 candidate genes for functional analysis and development of DNA constructs for repression or over-expression. Objective 5: Creation of transgenic melon lines, or transgenic heterologous systems (e.g. E. coli or tomato), to assess putative functions and potential as tools for molecular enhancement of melon fruit quality, using the candidate gene constructs from (4).
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Mevarech, Moshe, Jeremy Bruenn, and Yigal Koltin. Virus Encoded Toxin of the Corn Smut Ustilago Maydis - Isolation of Receptors and Mapping Functional Domains. United States Department of Agriculture, September 1995. http://dx.doi.org/10.32747/1995.7613022.bard.

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Ustilago maydis is a fungal pathogen of maize. Some strains of U. maydis encode secreted polypeptide toxins capable of killing other susceptible strains of U. maydis. Resistance to the toxins is conferred by recessive nuclear genes. The toxins are encoded by genomic segments of resident double-strande RNA viruses. The best characterized toxin, KP6, is composed of two polypeptides, a and b, which are not covalently linked. It is encoded by P6M2 dsRNA, which has been cloned, sequenced and expressed in a variety of systems. In this study we have shown that the toxin acts on the membranes of sensitive cells and that both polypeptides are required for toxin activity. The toxin has been shown to function by creating new pores in the cell membrane and disrupting ion fluxes. The experiments performed on artificial phospholipid bilayers indicated that KP6 forms large voltage-independent, cation-selective channels. Experiments leading to the resolution of structure-function relationship of the toxin by in vitro analysis have been initiated. During the course of this research the collaboration also yielded X-ray diffracion data of the crystallized a polypeptide. The effect of the toxin on the pathogen has been shown to be receptor-mediated. A potential receptor protein, identified in membrane fractions of sensitive cells, was subjected to tryptic hydrolysis followed by amino-acid analysis. The peptides obtained were used to isolate a cDNA fragment by reverse PCR, which showed 30% sequence homology to the human HLA protein. Analysis of other toxins secreted by U. maydis, KP1 and KP4, have demonstrated that, unlike KP6, they are composed of a single polypeptide. Finally, KP6 has been expressed in transgenic tobacco plants, indicating that accurate processing by Kex2p-like activity occurs in plants as well. Using tobacco as a model system, we determined that active antifungal toxins can be synthesized and targeted to the outside of transgenic plant cells. If this methodology can be applied to other agronomically crop species, then U. maydis toxins may provide a novel means for biological control of pathogenic fungi.
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Lers, Amnon, and Gan Susheng. Study of the regulatory mechanism involved in dark-induced Postharvest leaf senescence. United States Department of Agriculture, January 2009. http://dx.doi.org/10.32747/2009.7591734.bard.

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Postharvest leaf senescence contributes to quality losses in flowers and leafy vegetables. The general goal of this research project was to investigate the regulatory mechanisms involved in dark-induced leaf senescence. The regulatory system involved in senescence induction and control is highly complex and possibly involves a network of senescence promoting pathways responsible for activation of the senescence-associated genes. Pathways involving different internal signals and environmental factors may have distinctive importance in different leaf senescence systems. Darkness is known to have a role in enhancement of postharvest leaf senescence and for getting an insight into its regulatory mechanism/s we have applied molecular genetics and functional genomics approaches. The original objectives were: 1. Identification of dark-induced SAGs in Arabidopsis using enhancer/promoter trap lines and microarray approaches; 2. Molecular and functional characterization of the identified genes by analyzing their expression and examining the phenotypes in related knockout mutant plants; 3. Initial studies of promoter sequences for selected early dark-induced SAGs. Since genomic studies of senescence, with emphasis on dark-induced senescence, were early-on published which included information on potential regulatory genes we decided to use this new information. This is instead of using the uncharacterized enhancer/promoter trap lines as originally planned. We have also focused on specific relevant genes identified in the two laboratories. Based on the available genomic analyses of leaf senescence 10 candidate genes hypothesized to have a regulatory role in dark-induced senescence were subjected to both expression as well as functional analyses. For most of these genes senescence-specific regulation was confirmed, however, functional analyses using knock-out mutants indicated no consequence to senescence progression. The transcription factor WARK75 was found to be specifically expressed during natural and dark-induced leaf senescence. Functional analysis demonstrated that in detached leaves senescence under darkness was significantly delayed while no phenotypic consequences could be observed on growth and development, including no effect on natural leaf senescence,. Thus, WARKY75 is suggested to have a role in dark-induced senescence, but not in natural senescence. Another regulatory gene identified to have a role in senescence is MKK9 encoding for a Mitogen-Activated Protein Kinase Kinase 9 which is upregulated during senescence in harvested leaves as well as in naturally senescing leaves. MKK9 can specifically phosphorylate another kinase, MPK6. Both knockouts of MKK9 and MPK6 displayed a significantly senescence delay in harvested leaves and possibly function as a phosphorelay that regulates senescence. To our knowledge, this is the first report that clearly demonstrates the involvement of a MAP kinase pathway in senescence. This research not only revealed a new signal transduction pathway, but more important provided significant insights into the regulatory mechanisms underlying senescence in harvested leaves. In an additional line of research we have employed the promoter of the senescence-induced BFN1 gene as a handle for identifying components of the regulatory mechanism. This gene was shown to be activated during darkinduced senescence of detached leaves, as well as natural senescence. This was shown by following protein accumulation and promoter activity which demonstrated that this promoter is activated during dark-induced senescence. Analysis of the promoter established that, at least some of the regulatory sequences reside in an 80 bps long fragment of the promoter. Overall, progress was made in identification of components with a role in dark-induced senescence in this project. Further studies should be done in order to better understand the function of these components and develop approaches for modulating the progress of senescence in crop plants for the benefit of agriculture.
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Lers, Amnon, and Pamela J. Green. Analysis of Small RNAs Associated with Plant Senescence. United States Department of Agriculture, March 2013. http://dx.doi.org/10.32747/2013.7593393.bard.

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Senescence is an agriculturally significant process due to its negative impact to crop yield and postharvest quality. The genetic regulatory systems controlling senescence induction and progress respond to both developmental and environmental stress signals and involve numerous gene expression changes. Knowledge about the key molecular factors which control senescence is very limited. MicroRNAs (miRNAs) are a class of small RNAs which typically function by guiding cleavage of target messenger RNAs. They have been shown to play major roles in a variety of plant processes including development, responses to environmental stresses, and senescence. The long-term goal of this work is to elucidate roles of small RNAs associated with plant senescence. The hypothesis underlying this research is that miRNA-mediated regulation makes important contributions to the senescence process in plants. Specific, original research objectives included: 1) Profiling of small RNAs from senescing plants; 2) Data Analysis and public access via a user-friendly web interface; 3) Validation of senescence-associated miRNAs and target RNAs; 4) Development of transgenic plants for functional analysis of miRNAs in Arabidopsis. Major revisions made in the research compared to the original work plan included 1) Exclusion of the planned work with tomato as recommended by the BARD review panel; 2) Performing miRNA study also in senescing Arabidopsis siliques, in addition to senescing leaves. To identify senescenceregulation of miRNAs in Arabidopsis thaliana, eight small RNA libraries were constructed and sequenced at four different stages of development and senescence from both leaves and siliques, resulting in more than 200 million genome-matched sequences. Parallel Analysis of RNA Ends (PARE) libraries, which enable the large-scale examination of miRNA-guided cleavage products, were also constructed and sequenced, resulting in over 750 million genome-matched sequences. These massive datasets lead to the identification of new miRNAs, as well as new regulation of known miRNAs and their target genes during senescence, many of which have established roles in nutrient responsiveness and cell structural integrity. In keeping with remobilization of nutrients thought to occur during senescence, many miRNAs and targets had opposite expression pattern changes between leaf and silique tissues during the progression of senescence. Taken together, these findings highlight the integral role that miRNAs may play in the remobilization of resources and alteration of cellular structure that is known to occur in senescence. Experiments were initiated for functional analysis of specific senescence-associated miRNAs and respective target genes. Transgenic Arabidopsis plants were generated in which miR408, found in this study to be significantly induced in leaf senescence, was over-expressed either constitutively or under a senescence-specific promoter. These plants are currently being characterized for any altered phenotypes. In addition T-DNA knock out mutants for various target genes identified in this research are being analyzed. This work provides insights about specific miRNAs that contribute to leaf and silique senescence. The knowledge generated may suggest new strategies to monitor and alter the progression of senescence in crops for agricultural improvement.
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Tarasenko, Rostyslav O., Svitlana M. Amelina, and Albert A. Azaryan. Features of the use of cloud-based translation systems in the process of forming information competence of translators. [б. в.], September 2019. http://dx.doi.org/10.31812/123456789/3256.

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The current trends in the translator training are shown, which reflect the orientation towards the use of cloud-based automated translation systems. The possibilities of studying cloud-based translation systems in the educational process of training the translator are considered. The role of mastering modern translation tools for forming information competence of translators, particularly technological component, was described. The definition of the list and type of basic translation tools that should be mastered in the studying process was discussed. These tools should include automated translation systems and terminological management systems. It is advisable to provide for the study of both desktop and cloud-based systems. The inclusion in the content of the training translators the study of cloud-based systems of automated translation after desktop systems is proposed. A number of advantages of cloud-based translation systems for the use in the process of training the translators is defined and substantiated. A comparative analysis of the functional of cloud-based automated translation systems (Wordfast Anywhere, XTM Cloud, and MemSource) with the aim of including them in the content of the training program for translators has been carried out.
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Lers, Amnon, and Pamela J. Green. LX Senescence-Induced Ribonuclease in Tomato: Function and Regulation. United States Department of Agriculture, September 2003. http://dx.doi.org/10.32747/2003.7586455.bard.

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Natural leaf senescence, which occurs even when growth conditions are near optimal, has a negative influence on yield. Postharvest induced senescence contributes to the losses of quality in flowers, foliage, and vegetables. Strategies designed to control the senescence process in crop plants could therefore have great applied significance. However, the successful design of such strategies requires a better insight into the senescence machinery and control in higher plants. A main feature of senescence is the hydrolysis of macromolecules by hydrolases of various types such as ribonucleases (RNases) and proteases. Previously we had identified and characterized the tomato LX RNase gene demonstrating its transcript to be highly and specifically induced during senescence. This reported study was focused on LX but also had broadened our research to other senescence-associated nucleic acids degrading enzymes to learn about their function and the regulation of their encoding genes. Beside tomato we used parsley and Arabidopsis for the study of: the bi-functional nuclease which has a role in senescence. The study of different senescence- associated nucleases in few plant systems will allow a more general view on function and regulation of these enzymes in senescence. The specific original proposed objectives included: 1. Study the consequences of alterations in LX RNase level on tomato leaf senescence and general development; 2. Analyze stimuli which may participate in senescence-specific activation of the LX gene; 3. Clone the senescence-associated BFNI nuclease gene homologue from tomato. 4. Further characterize the sequences required for senescence-specific gene expression. Homozygous transgenic plants in which LX gene was either inhibited or over-expressed were generated. In both of these LX mutated plants no major phenotypic consequences were observed, which may suggests that LX is not essential for plant growth under optimal growth conditions. Lack of any abnormalities in the LX over-expressing lines suggests that special system exist to allow function of the RNase only when needed. Detailed analyses of growth under stress and consequences to RNA metabolism are underway. We have analyzed LX expression on the protein level demonstrating that it is involved also in petal senescing. Our results suggest that LX is responding to complex regulation involving developmental, organ dependent factors and responds differently to hormonal or environmental stimuli in the different plant organs. The cloned 1.4 kb promoter was cloned and its analysis revealed that probably not all required elements for senescence induction are included. Biochemical analysis of senescence-associated be-functional nucleases in the different plants, tomato, parsley and Arabidopsis, suggests they belong to a sub-class within the type I plant nucleases. The parsley PcNUC1/2 nuclease protein was purified from senescing leaves its and activity was studied in vitro revealing endo-, double strand, nucleolytic activity and exo-nucleolytic activity. Its encoding gene was cloned and found to be induced on the mRNA level. The promoter of the related Arabidopsis BFNI nuclease was shown in both tomato and Arabidopsis to be able and direct senescence-specific expression suggesting that, at least part, the gene is regulated on the transcriptional level and that the mechanism for this senescence-specific regulation is conserved between different plants. Few plants in which the BFNI gene is mutated were identified which are subjected now to detailed analysis. Our results suggest that the senescence-related nucleic acid degrading enzymes share similarities in both function and regulation between different plants and possibly have important functions in processes un-related to senescence. Still, the function of these enzymes, at least in some cases is not essential to plant development under optimal growth conditions. We are now at the stage which permits in depth investigation of the specific functions and mode of molecular regulation of senescence-associated nucleases with the aid of the research tools developed. The isolated senescence-specific promoter, shown to be active in heterologous plant system, could be utilized in agricultural-related biotechnological applications for retardation of senescence.
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Chang, Min Cheol, Yoo Jin Choo, and Sohyun Kim. Effect of Prehabilitation for Patients with Frailty Undergoing Colorectal Cancer Surgery: A Systematic Review and Meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, November 2022. http://dx.doi.org/10.37766/inplasy2022.11.0105.

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Review question / Objective: We performed a meta-analysis to assess the impact of prehabilitation before colorectal surgery on functional outcome and postoperative complications in patients with frailty. Condition being studied: Colorectal cancer is a common disease in the elderly, and over 65 years of age accounts for more than 50% of all patients with colorectal cancer. The patients with colorectal cancer surgery showed 8.7% major morbidity and mortality and 31.6% minor complications. The high complication rate of patients with colorectal surgery is related to the fact that there are many elderly patients. Frailty is common in elderly patients, and the frailty is associated with adverse perioperative outcomes. The frail patients with colorectal surgery showed worse postoperative morbidity, mortality and prolonged length of hospital stay. Although the frailty results from irresistible aging-associated decline in reserve and function across multiple physiologic systems, several attempts have been conducted to improve frailty in patients with colorectal cancer surgery and consequently improve the postoperative outcomes. Prehabilitation was one of these attempts for improving physical activity and postoperative outcomes on patients with frailty undergoing colorectal cancer surgery. So far, several studies conducted clinical trials for determining whether prehabilitation has positive effect on improving postoperative outcomes in patients with frailty undergoing colorectal surgery. However, the results of these previous studies are controversial.
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