Dissertations / Theses on the topic 'Functional analysis'
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Sanchez, Sindy. "What’s the Function? Assessing Correspondence between Functional Analysis Procedures." Scholar Commons, 2018. https://scholarcommons.usf.edu/etd/7361.
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In 1997, Congress established the Individuals with Disabilities Education Act (IDEA, 1997), which required that schools conduct functional behavior assessments when a student engages in problem behavior that may lead to suspension or expulsion (Ervin et al., 2001; Yell & Katsiyanis, 2010). As a result, research has expanded to include ways to adapt the functional assessment process in school settings. The purpose of this study was to compare the correspondence between functional analysis procedures for students in a private school and validate the assessment outcomes with interventions conducted in the classroom settings. The results indicate that both assessments corresponded in 87% of all functions identified in the study. Furthermore, the interventions yielded reductions in problem behaviors for all participants.
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Gibson, Christine M. "Response Patterns in Functional Analyses: a Preliminary Analysis." Thesis, University of North Texas, 2012. https://digital.library.unt.edu/ark:/67531/metadc149594/.
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Functional assessment procedures have proven effective in identifying the operant contingencies that maintain problem behavior. Typically, the evaluation of responding during functional analyses is conducted at the condition level. However, some variables affecting occurrences of behavior cannot be evaluated solely through the use of a cross-session analysis. Evaluating within-session patterns of responding may provide information about variables such as extinction bursts, discriminative stimuli, and motivating operations such as deprivation and satiation. The current study was designed to identify some typical response patterns that are generated when data are displayed across and within sessions of functional analyses, discuss some variables that may cause these trends, and evaluate the utility of within-session analyses. Results revealed that several specific patterns of responding were identified for both across- and within-session analyses, which may be useful in clarifying the function of behavior.
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Abrahamson, Krista. "History, Implementation, and Pedagogical Implications of an Updated System of Functional Analysis." Thesis, University of Oregon, 2016. http://hdl.handle.net/1794/20480.
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This dissertation follows the history of functional ideas and their pedagogy, illuminates with many examples the implementation of my updated system of Functional Analysis, and discusses the pedagogical implications that this updated system implies. The main goal is to update a system of labeling to be as pedagogically friendly as possible, in order to assist students and teachers of harmony to more easily and enjoyably learn, teach, and engage with common-practice tonal harmonic practice. Example syllabi, assignments, classroom demonstrations, and long projects are also included, and each aspect of the labeling is carefully discussed as it is presented.
By surveying the history of functional thinking in music theory, we find that desire to analyze for function is not a new idea, and has been a goal of many theorists and harmony teachers for centuries. However, the current methods for instructing in function still leave students confused or baffled, as they struggle to match functional concepts to labels that do not exemplify their analysis goals and methods that insist on starting from tiny detail instead of coming from a more complete musical perspective.
The elaboration of each detail of my Functional Analysis system shows how each part of Functional Analysis has been designed to help make harmonic analysis quicker, easier, more intuitive, and more personalized. The greater pedagogical implications on a
larger scale involving courses and curricula are also covered, informed by my experience both as a teacher of today’s standard system and from teaching Functional Analysis in the classroom.
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Hoffmann, Eva R. "Functional analysis of MLH1." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249558.
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Mosina, Leticia Leticia. "Structure-function analysis of a novel multi-functional glycoside hydrolase." Thesis, University of Pretoria, 2019. http://hdl.handle.net/2263/77859.
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The present study presents the research findings of the first ever multi-modular Paenibacillus mucilaginosus glycoside hydrolase (PmGH). Furthermore, we report the successful crystallisation of a multi-modular GH. The GH is composed of two catalytic modules (GH5 and GH6) and two carbohydrate binding modules (both CBM3). Functional analysis demonstrated that the cellulase, mannanase and xylanase activities of PmGH (130 kDa) were attributed to the GH5 catalytic domain. The presence of the GH6 catalytic domain resulted in slightly increased cellulase activity in PmGH. Optimal PmGH activity and functional stability was highest at pH 4-6 and at 40-60°C The structural properties of PmGH that determine its robust nature were further investigated.
Homology modelling of PmGH showed the GH5 and GH6 domains to be independent but provided no structural information for the CBMs and linker regions. However, successful homology modelling of the individual domains indicated that the combination of the modules makes PmGH structurally and functionally novel. Glycoside hydrolases occur as independent modules or as part of a multi-modular protein with other catalytic and/or non-catalytic modules. Multiple combinations of these modules can occur in nature resulting in novel proteins such as PmGH.
In an attempt to determine the PmGH crystal structure, a range of crystallisation conditions were tested. After extensive screening and optimisation, multiple PmGH crystals were diffracted, using both local diffraction and Synchrotron radiation sources (ESRF, France). Overall ~90% of the PmGH protein crystals did not diffract and of the remaining ~10% yielded unsatisfactory data. Phasing by molecular replacement also yielded no structural solutions. Alternative phasing methods such as multi-wavelength anomalous dispersion were also unsuccessful due to the quality of the diffraction data collected. Given the severe lack of multi-modular GH crystal structures in protein structure databases, the present study highlights the major limitations in structural studies of these important enzymes.Thesis (PhD)--University of Pretoria, 2018.
Genetics
PhD
Unrestricted
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Humphries, Donna Irene Nisbet. "Canadian universities : a functional analysis." Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/29672.
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This thesis identifies a university's typical administrative structure for the purpose of establishing a framework which working university archivists can use to acquire control of university records. The organizational structure of Canadian universities is examined with respect to their functions, juridical persons, and their relative competences. This study may be defined as a "functional analysis."
The intertwined concepts of function, competence, and juridical persons serve as foundations for this thesis. A function is defined as the whole of the activities, considered abstractly, necessary to accomplish one purpose. A competence is the authority to carry out a determined sphere of activities within one function. Such authority, however, has to be delegated or assigned to a given office or individual, and that office or individual is termed a juridical person. Therefore, a link is forged between a function and a competence through a juridical person, because it is a juridical person who carries out certain duties and responsibilities within a specified function.
Since juridical persons create records in the course of executing their competence, a functional analysis establishes the provenance of the records and places the records of an administrative body in the context of their creation. A functional analysis also reveals and explains the relationships and bonds between the records, record series, and record
groups that comprise an administration's archival residue. These objectives -- understanding the organizational structure of the administrative body, identifying its functions, determining the provenance of its records, and placing records in the context of the activities that generate them — help archivists and records managers acquire a fundamental level of intellectual control over the administrative body's records. Without this knowledge, archivists and records managers cannot proceed with any of their own practices.
By studying the history and development of universities from the Middles Ages to the twentieth century, this thesis identifies four functions which are common to all universities: Sustaining Itself, Teaching, Research, and Service to the Community. A number of juridical persons, either in the form of administrative bodies or individuals who comprise the administrative structure of the university, are then examined, and the functions with which with they are entrusted are ascertained by studying their competences. As a result of this analysis, the typical organizational structure of a university is revealed, the functional provenance of records created by universities (as a whole) are identified, and its records are placed in the context of the activities that generate them.Arts, Faculty of
Library, Archival and Information Studies (SLAIS), School of
Graduate
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Izquierdo, Ángel Cabrera. "A functional analysis of categorization." Diss., Georgia Institute of Technology, 1995. http://hdl.handle.net/1853/30522.
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Suk, Hye Won. "Functional generalized structured component analysis." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=117051.
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The invention of sophisticated measurement tools, such as motion capture devices, handheld computers, Bluetooth devices, eye-trackers, and brain scanners, has facilitated the collection of functional data that can be considered to arise from an underlying smooth function varying over a continuum such as time and space. Functional data analysis (FDA) is an emerging branch of statistics, which develops and applies statistical methods for the analysis of such types of data. Various FDA methods have been proposed by extending traditional multivariate statistical methods to accommodate functional data. Nonetheless, there has been little attempt to develop functional extensions of structural equation modeling (SEM), in spite of the remarkable popularity of SEM in various disciplines due to its flexibility of modeling complex relationships among observed and latent variables. This thesis thus aims to propose a general framework for functional SEM, called functional generalized structured component analysis (functional GSCA), to examine a variety of hypothesized relationships among observed and latent variables, while permitting observed variables to be functional rather than multivariate. The thesis begins by describing GSCA and penalized least squares smoothing as the two basic building blocks of the proposed method. Subsequently, it provides the technical details of the proposed method. The model for functional GSCA is provided and a penalized least squares criterion is developed for parameter estimation, which is minimized by an alternating penalized least squares algorithm. The thesis also demonstrates the usefulness of the proposed method by analyzing synthetic and real data sets. It concludes with discussions on limitations and possible extensions of the proposed method.L'invention d'outils de mesures sophistiqués tel que les appareils de capture de mouvements, les ordinateurs portables, les appareils Bluetooth, l'oculométrie et les scanners cérébraux ont facilité la compilation de données fonctionnelles qui peuvent être considérées comme provenant d'une fonction variant sur un continuum tel que l'espace et le temps. L'analyse de données fonctionnelles (ADF) est une discipline émergente des statistiques, qui développe et applique les méthodes statistiques pour l'analyse de ce type de données. Plusieurs méthodes d'ADFs ont été proposées en prolongeant les méthodes traditionnelles de statistiques multi variées pour s'adapter aux données multifonctionnelles. Toutefois, peu de tentatives ont été effectuées dans le développement des extensions fonctionnelles des modèles d'équations structurelles (MES), malgré la popularité significative des MESs en plusieurs disciplines grâce à sa souplesse de la modélisation des relations complexes entre les variables observées et latentes. Cette thèse a donc pour objectif de proposer un cadre général pour les MESs fonctionnels, appelé l'analyse en composantes structurée généralisée fonctionnelle (ASSG fonctionnelle), qui combine l'analyse en composantes structurée généralisée fonctionnelle avec les moindres carrés pénalisés lissés par la fonction spline dans un cadre unifié. La méthode proposée peut être utilisée pour analyser une variété de relations hypothétiques entre des variables observées et latentes, tout en permettant aux variables observées d'être fonctionnelles plutôt que scalaires. La thèse commence en décrivant l'ACSG et les moindres carrés pénalisés lisser par la fonction spline tel que les deux parties constituantes de la méthode proposée. Le modèle pour l'ACSG fonctionnelle est apporté et le critère des moindres carrés pénalisés sont développés par une estimation paramétrique, qui est minimisé par un algorithme alternatif de moindres carrés pénalisés. La thèse démontre également l'utilité de la méthode proposée par l'analyse de base de données réelles et synthétiques. En conclusion, sont présentées les discussions, limites et possibles extensions de la méthode proposée.
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Ding, Z. "Functional analysis of IP3 receptors." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.598546.
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I developed a high-throughput fluorescence polarisation (FP) binding assay using fluorescein-IP3 and purified N-terminal fragments of IP3R to examine the thermodynamics of ligand binding to IP3R. I demonstrate that at 4°C, equilibrium competition binding using 3H-IP3 and the FP assay provided similar estimates of the equilibrium dissociation constants (KD) for a variety of ligands. I showed that the IBC alone is sufficient for high-affinity binding of adenophostin A (AdA). Similar amounts of binding energy are diverted into rearranging the SD for IP3 and AdA, but they are distinguished by their binding enthalpy and entropy changes. I revealed that the enthalpy and entropy changes of the binding of 2-O-modified IP3 analogues are different, despite their similar free energy changes. These results prompted me to propose a new model to explain partial agonism of IP3R. Different cell-surface receptors have been reported to evoke Ca2+ release from different IP3-sensitive Ca2+ stores. I examined this phenomenon in fura 2-loaded HEK cells. Combined maximal concentrations of ATP and carbachol (CCh) evoked a Ca2+ response that was larger than the response to either alone, but smaller than the sum of the responses. In the absence of extracellular Ca2+, ATP evoked a Ca2+ release that was significantly larger than CCh in cells pretreated with CCh, but smaller than the ATP response in naïve cells. In the absence of extracellular Ca2+, CCh evoked a Ca2+ release that was significantly larger than ATP in cells pretreated with ATP, but smaller than the CCh response in naïve cells. These results suggest the existence of discrete agonist-specific Ca2+-stores that partially overlap.
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Georgiev, Plamen. "Functional analysis of Drosophila TRPM." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.611931.
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Blower, G. "A topic in functional analysis." Thesis, University of Oxford, 1989. http://ora.ox.ac.uk/objects/uuid:0724199d-41fd-48f1-882c-19602576a2a0.
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We introduce the class AUMD of Banach spaces X for which X-valued analytic martingales converge unconditionally. We shew that various possible definitions of this class are equivalent by methods of martingale decomposition. We shew that such X have finite cotype and are q-complex uniformly convex in the sense of Garling. Using multipliers we shew that analytic martingales valued in L1 converge unconditionally and that AUMD spaces have the analytic Radon-Nikodym property. We shew that X has the AUMD property if and only if strong Hbrmander-Mihlin multipliers are bounded on the Hardy space H1x(T). We achieve this by representing multipliers as martingale transforms. It is shewn that if X is in AUMD and is of cotype two then X has the Paley Theorem property. Using an isomorphism result we shew that if A is an injective operator system on a separable Hilbert space and P a completely bounded projection on A, then either PA or (I-P)A is completely boundedly isomorphic to A. The finite-dimensional version of this result is deduced from Ramsey's Theorem. It is shewn that B(e2 is primary. It is shewn that weakly compact homomorphisms T from the 2 disc algebra into B(e2 are necessarily compact. An explicit form for such T is obtained using spectral projections and it is deduced that such T are absolutely summing.
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Bashton, Matthew. "Functional analysis of domain combinations." Thesis, University of Cambridge, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.616149.
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Hadjipantelis, Pantelis-Zenon. "Functional data analysis in phonetics." Thesis, University of Warwick, 2013. http://wrap.warwick.ac.uk/62527/.
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The study of speech sounds has established itself as a distinct area of research, namely Phonetics. This is because speech production is a complex phenomenon mediated by the interaction of multiple components of a linguistic and non-linguistic nature. To investigate such phenomena, this thesis employs a Functional Data Analysis framework where speech segments are viewed as functions. FDA treats functions as its fundamental unit of analysis; the thesis takes advantage of this, both in conceptual as well as practical terms, achieving theoretical coherence as well as statistical robustness in its insights. The main techniques employed in this work are: Functional principal components analysis, Functional mixed-effects regression models and phylogenetic Gaussian process regression for functional data. As it will be shown, these techniques allow for complementary analyses of linguistic data. The thesis presents a series of novel applications of functional data analysis in Phonetics. Firstly, it investigates the influence linguistic information carries on the speech intonation patterns. It provides these insights through an analysis combining FPCA with a series of mixed effect models, through which meaningful categorical prototypes are built. Secondly, the interplay of phase and amplitude variation in functional phonetic data is investigated. A multivariate mixed effects framework is developed for jointly analysing phase and amplitude information contained in phonetic data. Lastly, the phylogenetic associations between languages within a multi-language phonetic corpus are analysed. Utilizing a small subset of related Romance languages, a phylogenetic investigation of the words' spectrograms (functional objects defined over two continua simultaneously) is conducted to showcase a proof-of-concept experiment allowing the interconnection between FDA and Evolutionary Linguistics.
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Haw, Robin Andrew. "Functional analysis of yeast RAP1." Thesis, University of Nottingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285773.
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Turnbull, Emma. "Functional analysis of S.pombe Cdc37." Thesis, University of Edinburgh, 2005. http://hdl.handle.net/1842/14594.
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The Schizosaccharomyces pombe cdc37 gene has been identified and was found to be essential for cell viability. To further study S. pombe Cdc37, a range of mutants were generated using both random and directed mutagenesis. Cdc37 temperature-sensitive mutant cells at the restrictive temperature stop dividing within a single cell cycle. Cdc37 protein levels were not changed at the non-permissive temperature in mutants, indicating that cell inviability arises from defective function of the mutant Cdc37 protein. Morphologically, temperature-sensitive mutant cells arrest with a uniform phenotype, being elongated, characteristic of the cdc phenotype. About 80% of these elongated cells contained a single nucleus with a 2C DNA content, indicating that cdc37 temperature-sensitive mutants arrest in G2. The only exception was cdc37-J, where half the cells leaked through to mitosis after 8 hours at the restrictive temperature and displayed divided nuclei and septa, arresting with defects in cytokinesis. Characterisation of the cdc37 temperature-sensitive mutants led to the identification of Cdc2 as a client protein and a principal candidate for the cause of the G2 cell cycle arrest. Cdc2 activity was dramatically reduced within one hour at the non-permissive temperature in cdc37 temperature-sensitive mutants, but Cdc2 protein levels remained constant. Biochemical and genetic interactions between Cdc37 and Cdc2 were observed, supporting the idea of Cdc2 as a client of Cdc37. Cdc37 is believed to deliver client protein kinases to the co-chaperone Hsp90, forming a heterocomplex. A small fraction of total cellular Cdc37 in s. pombe forms a high molecular weight complex which also contains Hsp90, Cdc37 was found to biochemically and genetically interact with Hsp90, indicating these co-chaperones co-operate in S. pombe.
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Ottaviano, Raffaele. "Functional analysis of 5-hydroxymethylcytosine." Thesis, University of Edinburgh, 2014. http://hdl.handle.net/1842/17869.
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Mammalian DNA methylathion is a chemical reaction catalyzed by DNA methyltransferases (DNMTs) and involves the addition of a methyl group from the methyl donor SAM to the carbon 5 position of cytosine (C) in a CpG dinucleotide. Specifically, DNA methylation is essential for normal development and is involved in numerous key mechanisms such as genomic imprinting, X-chromosome inactivation, suppression of repetitive elements and may be involved in the regulation of single-copy gene expression. In the human genome the majority of CpGs are methylated whereas regions with high density of CpG sites, termed CpG islands and often co-localized within gene promoters, are typically free of this mark. Recently, a new modified cytosine, 5-hydroxymhetylcytosine (5-hmC), was identified and found at significant levels in mouse brain and both mouse and human embryonic stem (ES) cells. The conversion of 5-mC to 5-hmC is catalyzed by the ten-eleven translocation (TET) proteins of the 2-oxoglutarate (2OG)-and Fe(II)-dependent oxygenase superfamily. Many studies were conducted since the identification of 5-hmC and significant levels of 5-mC hydroxylation were found in many other mouse and human tissues. Importantly, many of the techniques used for 5-mC detection, such as bisulphite sequencing and methyl-sensitive restriction digestion, are incapable of distinguishing between 5mC and 5hmC implying the necessity not only to develop techniques specific for 5-hmC characterization but also reevaluation of previously published 5mC data. The biological function of 5-hmC is unknown however many recent studies have suggested a role for 5-hmC as an intermediate of either passive or active demethylation. The majority of studies of 5- hmC and TETs have used mouse ES cells as model system. Therefore, very little is known about 5-hmC patterns and TET expression within and between normal tissues. During my PhD, I used the recently developed 5-hmC-specific antibody for tiling microarrays and 5hmC-qPCR to examine both global 5hmC content and locus-specific patterns of 5hmC in several normal human tissues and breast cancer. I found that global 5-hmC content is highly variable between tissues compared to global 5-mC content. Moreover, TETs genes are highly expressed in most of tissues tested. Importantly, both global 5-hmC content and TETs genes are rapidly and significantly reduced as consequence of adaptation of cells from normal human tissue to cell culture. Using the 5hmC-specific antibody for tiling microarrays and 5-hmC-qPCR to profile locus-specific patterns of 5hmC, I found that 5-hmC patterns are tissue-specific in human samples. In addition, comparing array data to RNA-seq data, 5- hmC was found to co-localize at gene bodies of active genes. Moreover, despite the global 5-hmC reduction in cell lines, 5-hmC content remains enriched in some specific loci. In summary, my results show that tissue type is a major modifier of both global and locus-specific 5hmC at genes in normal human tissues. Furthermore, I also show that both TET gene expression and 5hmC content are significantly reduced and 5-hmC profiles reprogrammed during the passage from tissues to cell culture.
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Senior, Steven L. "Functional analysis of alpha-synuclein." Thesis, University of Oxford, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670161.
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Mountjoy, Jon-Dean. "Static analysis of functional languages." Thesis, Rhodes University, 1994. http://hdl.handle.net/10962/d1006690.
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Static analysis is the name given to a number of compile time analysis techniques used to automatically generate information which can lead to improvements in the execution performance of function languages. This thesis provides an introduction to these techniques and their implementation. The abstract interpretation framework is an example of a technique used to extract information from a program by providing the program with an alternate semantics and evaluating this program over a non-standard domain. The elements of this domain represent certain properties of interest. This framework is examined in detail, as well as various extensions and variants of it. The use of binary logical relations and program logics as alternative formulations of the framework , and partial equivalence relations as an extension to it, are also looked at. The projection analysis framework determines how much of a sub-expression can be evaluated by examining the context in which the expression is to be evaluated, and provides an elegant method for finding particular types of information from data structures. This is also examined. The most costly operation in implementing an analysis is the computation of fixed points. Methods developed to make this process more efficient are looked at. This leads to the final chapter which highlights the dependencies and relationships between the different frameworks and their mathematical disciplines.KMBT_223
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Kolle, Gabriel Victor. "Functional analysis of vertebrate Crim1 /." [St. Lucia, Qld.], 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16804.pdf.
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Vermeulen, J. J. C. "Constructive techniques in functional analysis." Thesis, University of Sussex, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.712698.
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Toenjes, Kurt Alan 1965. "Functional analysis of yeast fimbrin." Diss., The University of Arizona, 1998. http://hdl.handle.net/10150/288802.
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The actin cytoskeleton has been implicated in the structural and mechanical properties of the cytoplasmic matrix. Actin and a number of actin associated proteins work in concert to carry out the various functions of the actin cytoskeleton. However, it is unclear how actin associated proteins function in conjunction with actin in vivo. I used Saccharomyces cerevisiae to investigate the actin cytoskeleton (1, 2, 3). Sac6 protein (Sac6p) is an actin bundling protein that consists of a head domain and two homologous actin binding domains (ABDs) (4). Despite their homology, evidence exists that there are functional differences between the ABDs. To explore these differences I asked if either ABD could function in place of the other by creating chimeric proteins with different combinations of the ABDs. When tested for function in vivo, these chimeric proteins are unable to complement the temperature and osmotic sensitivity of the sac6 null. This suggested that the ABDs of Sac6p are functionally distinct. To explore what functional differences exist between the ABDs of Sac6p, I made several truncations of Sac6p: a C-terminal deletion of Sac6p that retain the head and the first ABD (N410), and two different N-terminal deletions that contain only the second ABD (C386 & C397). Overexpression of N410 gave rise to a different organization of the actin cytoskeleton than did C386 or C397. This suggested that the ABDs/actin interactions are different. To determine whether the differences observed between the ABDs is the result of their interaction with actin, a method was developed to use allele specific suppression of the overexpression phenotype to define the region of interaction between the ABDs and actin. I tested full length Sac6p, N410, C386, and C397. The regions of actin implicated by suppression of the Sac6p overexpression and by allele specific suppression of sac6 mutants were similar. This similarity supports the validity of these two methods in mapping the regions of interaction between two proteins. Overexpression of N410 was suppressed by different actin mutations than overexpression of C386 or C397 suggesting that differences exist between the Sac6p actin binding domains in their interaction with actin.
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Nicklow, Katelyn E. "EFFICIENT IDENTIFICATION OF FUNCTION: A COMPARISON OF DIFFERENT IMPLEMENTERS DURING FUNCTIONAL ANALYSES." UKnowledge, 2019. https://uknowledge.uky.edu/edsrc_etds/76.
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Functional analyses (FAs) are a common tool used in the assessment and treatment of severe problem behaviors and often occur in the context of clinical settings with unfamiliar, trained staff. Previous research suggests that inconsistent outcomes can emerge when caregivers with an existing history of seeing their child’s challenging behavior are trained to implement the assessment in place of clinical staff. The purpose of the current study was to expand on existing literature by comparing FA implemented by clinical staff and caregivers in the context of a clinical setting. Results demonstrate that efficient identification of function and differentiated rates of problem behavior given the inclusion of caregivers during assessment may vary based on the child’s existing history of responding with those caregivers. Implications of results for researchers and practitioners are discussed.
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Schwartz, Yannick. "Large-scale functional MRI analysis to accumulate knowledge on brain functions." Thesis, Paris 11, 2015. http://www.theses.fr/2015PA112056/document.
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Comment peut-on accumuler de la connaissance sur les fonctions cérébrales ? Comment peut-on bénéficier d'années de recherche en IRM fonctionnelle (IRMf) pour analyser des processus cognitifs plus fins et construire un modèle exhaustif du cerveau ? Les chercheurs se basent habituellement sur des études individuelles pour identifier des régions cérébrales recrutées par les processus cognitifs. La comparaison avec l'historique du domaine se fait généralement manuellement pas le biais de la littérature, qui permet de définir des régions d'intérêt dans le cerveau. Les méta-analyses permettent de définir des méthodes plus formelles et automatisables pour analyser la littérature. Cette thèse examine trois manières d'accumuler et d'organiser les connaissances sur le fonctionnement du cerveau en utilisant des cartes d'activation cérébrales d'un grand nombre d'études. Premièrement, nous présentons une approche qui utilise conjointement deux expériences d'IRMf similaires pour mieux conditionner une analyse statistique. Nous montrons que cette méthode est une alternative intéressante par rapport aux analyses qui utilisent des régions d'intérêts, mais demande cependant un travail manuel dans la sélection des études qui l'empêche de monter à l'échelle. A cause de la difficulté à sélectionner automatiquement les études, notre deuxième contribution se focalise sur l'analyse d'une unique étude présentant un grand nombre de conditions expérimentales. Cette méthode estime des réseaux fonctionnels (ensemble de régions cérébrales) et les associe à des profils fonctionnels (ensemble pondéré de descripteurs cognitifs). Les limitations de cette approche viennent du fait que nous n'utilisons qu'une seule étude, et qu'elle se base sur un modèle non supervisé qui est par conséquent plus difficile à valider. Ce travail nous a cependant apporté la notion de labels cognitifs, qui est centrale pour notre dernière contribution. Cette dernière contribution présente une méthode qui a pour objectif d'apprendre des atlas fonctionnels en combinant plusieurs jeux de données. [Henson2006] montre qu'une inférence directe, c.a.d. la probabilité d'une activation étant donné un processus cognitif, n'est souvent pas suffisante pour conclure sur l'engagement de régions cérébrales pour le processus cognitif en question. Réciproquement, [Poldrack 2006] présente l'inférence inverse qui est la probabilité qu'un processus cognitif soit impliqué étant donné qu'une région cérébrale est activée, et décrit le risque de raisonnements fallacieux qui peuvent en découler. Pour éviter ces problèmes, il ne faut utiliser l'inférence inverse que dans un contexte où l'on suffisamment bien échantillonné l'espace cognitif pour pouvoir faire une inférence pertinente. Nous présentons une méthode qui utilise un « meta-design » pour décrire des tâches cognitives avec un vocabulaire commun, et qui combine les inférences directe et inverse pour mettre en évidence des réseaux fonctionnels qui sont cohérents à travers les études. Nous utilisons un modèle prédictif pour l'inférence inverse, et effectuons les prédictions sur de nouvelles études pour s'assurer que la méthode n'apprend pas certaines idiosyncrasies des données d'entrées. Cette dernière contribution nous a permis d'apprendre des réseaux fonctionnels, et de les associer avec des concepts cognitifs. Nous avons exploré différentes approches pour analyser conjointement des études d'IRMf. L'une des difficultés principales était de trouver un cadre commun qui permette d'analyser ensemble ces études malgré leur diversité. Ce cadre s'est instancié sous la forme d'un vocabulaire commun pour décrire les tâches d'IRMf. et a permis d'établir un modèle statistique du cerveau à grande échelle et d'accumuler des connaissances à travers des études d'IRM fonctionnelleHow can we accumulate knowledge on brain functions? How can we leverage years of research in functional MRI to analyse finer-grained psychological constructs, and build a comprehensive model of the brain? Researchers usually rely on single studies to delineate brain regions recruited by mental processes. They relate their findings to previous works in an informal way by defining regions of interest from the literature. Meta-analysis approaches provide a more principled way to build upon the literature. This thesis investigates three ways to assemble knowledge using activation maps from a large amount of studies. First, we present an approach that uses jointly two similar fMRI experiments, to better condition an analysis from a statistical standpoint. We show that it is a valuable data-driven alternative to traditional regions of interest analyses, but fails to provide a systematic way to relate studies, and thus does not permit to integrate knowledge on a large scale. Because of the difficulty to associate multiple studies, we resort to using a single dataset sampling a large number of stimuli for our second contribution. This method estimates functional networks associated with functional profiles, where the functional networks are interacting brain regions and the functional profiles are a weighted set of cognitive descriptors. This work successfully yields known brain networks and automatically associates meaningful descriptions. Its limitations lie in the unsupervised nature of this method, which is more difficult to validate, and the use of a single dataset. It however brings the notion of cognitive labels, which is central to our last contribution. Our last contribution presents a method that learns functional atlases by combining several datasets. [Henson 2006] shows that forward inference, i.e. the probability of an activation given a cognitive process, is often not sufficient to conclude on the engagement of brain regions for a cognitive process. Conversely, [Poldrack 2006] describes reverse inference as the probability of a cognitive process given an activation, but warns of a logical fallacy in concluding on such inference from evoked activity. Avoiding this issue requires to perform reverse inference with a large coverage of the cognitive space. We present a framework that uses a "meta-design" to describe many different tasks with a common vocabulary, and use forward and reverse inference in conjunction to outline functional networks that are consistently represented across the studies. We use a predictive model for reverse inference, and perform prediction on unseen studies to guarantee that we do not learn studies' idiosyncrasies. This final contribution permits to learn functional atlases, i.e. functional networks associated with a cognitive concept. We explored different possibilities to jointly analyse multiple fMRI experiments. We have found that one of the main challenges is to be able to relate the experiments with one another. As a solution, we propose a common vocabulary to describe the tasks. [Henson 2006] advocates the use of forward and reverse inference in conjunction to associate cognitive functions to brain regions, which is only possible in the context of a large scale analysis to overcome the limitations of reverse inference. This framing of the problem therefore makes it possible to establish a large statistical model of the brain, and accumulate knowledge across functional neuroimaging studies
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Harper, John-Paul. "The class number one problem in function fields." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53619.
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Thesis (MComm)--Stellenbosch University, 2003.ENGLISH ABSTRACT: In this dissertation I investigate the class number one problem in function fields. More precisely I give a survey of the current state of research into extensions of a rational function field over a finite field with principal ring of integers. I focus particularly on the quadratic case and throughout draw analogies and motivations from the classical number field situation. It was the "Prince of Mathematicians" C.F. Gauss who first undertook an in depth study of quadratic extensions of the rational numbers and the corresponding rings of integers. More recently however work has been done in the situation of function fields in which the arithmetic is very similar. I begin with an introduction into the arithmetic in function fields over a finite field and prove the analogies of many of the classical results. I then proceed to demonstrate how the algebra and arithmetic in function fields can be interpreted geometrically in terms of curves and introduce the associated geometric language. After presenting some conjectures, I proceed to give a survey of known results in the situation of quadratic function fields. I present also a few results of my own in this section. Lastly I state some recent results regarding arbitrary extensions of a rational function field with principal ring of integers and give some heuristic results regarding class groups in function fields.
AFRIKAANSE OPSOMMING: In hierdie tesis ondersoek ek die klasgetal een probleem in funksieliggame. Meer spesifiek ondersoek ek die huidige staat van navorsing aangaande uitbreidings van 'n rasionale funksieliggaam oor 'n eindige liggaam sodat die ring van heelgetalle 'n hoofidealgebied is. Ek kyk in besonder na die kwadratiese geval, en deurgaans verwys ek na die analoog in die klassieke getalleliggaam situasie. Dit was die beroemde wiskundige C.F. Gauss wat eerste kwadratiese uitbreidings van die rasionale getalle en die ooreenstemende ring van heelgetalle in diepte ondersoek het. Onlangs het wiskundiges hierdie probleme ook ondersoek in die situasie van funksieliggame oor 'n eindige liggaam waar die algebraïese struktuur baie soortgelyk is. Ek begin met 'n inleiding tot die rekenkunde in funksieliggame oor 'n eindige liggaam en bewys die analogie van baie van die klassieke resultate. Dan verduidelik ek hoe die algebra in funksieliggame geometries beskou kan word in terme van kurwes en gee 'n kort inleiding tot die geometriese taal. Nadat ek 'n paar vermoedes bespreek, gee ek 'n oorsig van wat alreeds vir quadratiese funksieliggame bewys is. In hierdie afdeling word 'n paar resultate van my eie ook bewys. Dan vermeld ek 'n paar resultate aangaande algemene uitbreidings van 'n rasionale funksieliggaam oor 'n eindige liggaam waar die van ring heelgetalle 'n hoofidealgebied is. Laastens verwys ek na 'n paar heurisitiese resultate aangaande klasgroepe in funksieliggame.
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Griesenauer, Erin. "Algebras of cross sections." Diss., University of Iowa, 2016. https://ir.uiowa.edu/etd/2086.
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My research studies algebras of holomorphic functions from $d$-tuples of $n\times n$- matrices, $M_n(\bC)^d$, to $M_n(\bC)$. In particular, I study the holomorphic functions that can be approximated by \emph{polynomial matrix concomitants}, that is polynomial maps from $M_n(\bC)^d$ to $M_n(\bC)$ that satisfy the relationship
\[
f(g^{-1}\fz g) = g^{-1}f(\fz)g
\]
for every $\fz \in M_n(\bC)^d$ and $g\in GL_n(\bC)$. In a sense, these are the polynomial maps that “remember” the structure of the $d$-tuple $\fz$.
My first result is that these holomorphic matrix concomitants can be identified with holomorphic cross sections of certain matrix bundles. A holomorphic matrix bundle is a fibred space in which every fibre is $M_n(\bC)$ and the fibres are glued together in such a way that the total space has a holomorphic structure.
Once the identification between holomorphic cross sections and holomorphic concomitants is established, the structure of the matrix bundle is used to endow the algebra of continuous cross sections with a $C^*$-algebra structure. Then we study the subalgebra of cross sections that can be approximated by polynomial concomitants. By identifying the matrix concomitants with cross sections, we are able to prove interesting results about these algebras.
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Akkoyun, Emrah. "Parallelization Of Functional Flow To Predict Protein Functions." Master's thesis, METU, 2011. http://etd.lib.metu.edu.tr/upload/12612932/index.pdf.
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Protein-protein interaction networks provide important information about what the biological function of proteins whose roles are unknown might be in a cell. These interaction networks were analyzed by a variety of approaches by running them on a single computer and the roles of the proteins identified were used to predict the function of the proteins unidentified. The functional flow is an approach that takes the network connectivity, distance effect, topology of the network with local and global views into account. With these advantages, that the functional flow produces more accurate results on the prediction of protein functions was presented by the previos conducted researches. However, the application implemented for this approach could not be practically applied on the large and complex network produced for the complex species because of memory limitation. The purpose of this thesis is to provide a new application be implemented on the high computing performance where the application can be scaled on the large data sets. Therefore, Hadoop, one of the open source map/reduce environments, was installed on 18 hosts each of which has eight cores.
Methodthe first map/reduce job distributes the protein interaction network as a format which allows parallel distributed computing to all the worker nodes, the other map/reduce job generates flows for each known protein function and the role of the proteins unidentified are predicted by accumulating all of these generated flows. It has been observed in the experiments we performed that the application requiring high performance computing can be decomposed into worker nodes efficiently and the application can provide better performance as the resources increase.
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Chadman, Corey S. "Functional Limits in Topology." Youngstown State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1371035042.
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Benton, Peter Nicholas. "Strictness analysis of lazy functional programs." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.281891.
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Ji, Boyang. "Comparative and Functional Genome Analysis of Magnetotactic Bacteria." Thesis, Aix-Marseille, 2013. http://www.theses.fr/2013AIXM4065.
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Les bactéries magnétotactiques (MTB) appartiennent à différents phyla procaryotes et ont la capacité de synthétiser des magnetosomes (cristaux de magnétite entourés par une membrane). Durant la thèse, nous avons procédé à l’analyse génomique de 2 bactéries magnétotactiques: Magnetospira sp. QH-2 et Magnetococcus MO-1. La synthénie et la correlation génique des gènes impliqués dans la formation des magnétosomes montrent que l'insertion de cet îlot chez QH-2 a eu lieu après la divergence entre les Magnetospirillum sp et Magnetospira sp. L'analyse comparative a mis en évidence trois groupes distincts de MTB : Groupe I, comprenant les souches Magnetospirillum spp. et Magnetospira; Groupe II avec MO-1 et M. marinus MC-1 et le Groupe III, avec D. magneticus RS-1. QH-2 montre aussi une évolution adaptative distincte par comparaison aux souches marines ou d'eau douce. L'analyse comparative des réseaux métaboliques révèle une très grande similitude intra-Groupe et une importante variabilité inter-Groupe. Cela est probablement dû aux enzymes impliqués dans les voies métaboliques anoxiques, qui représentent ainsi la contrainte à une distribution taxonomique large des MTB. Ces enzymes permettent ainsi de prédire le phénotype métabolique nécessaire à la production des magnétosomes. Différentes analyses (des protéines ribosomales au genome entier) indiquent une composition taxonomique chimérique des gènes de MO-1 et MC-1, et peut représenter une nouvelle lignée taxonomique chez les Protéobactéries. J’ai aussi participé à l'analyse des génomes de deux bactéries piezophiles, d’une bactérie photosynthétique pourpre et l’analyse phylogénomique des tyrosine-Kinases bactériennesMagnetotactic bacteria (MTB) are a diverse group of aquatic prokaryotes, which synthesize membrane-Enclosed magnetic crystals known as magnetosomes. In this thesis, the genome sequences of two marine MTB strains, Magnetospira sp. QH-2 and magneto-Ovoid strain MO-1 were analyzed. The magnetosome gene cluster synteny and mam gene correlation indicate that the insertion of the magnetosome island into QH-2 chromosome occurred after divergence between freshwater and marine magnetospirilla. Comparative genomic analysis revealed three distinct groups of sequenced MTB strains: Group I with Magnetospirillum spp. strains and Magnetospira strain, Group II with MO-1 strain and M. marinus MC-1, and Group III including Desulfovibrio magneticus RS-1. In addition, it shows an adaptive evolution of two marine MTB strains to marine sediments in comparison with closely related freshwater species. Moreover, comparative metabolic network analysis reveals high level of intra-Group similarity and inter-Group variety in MTB. With anoxic network enzymes, potential “MTB” strains are predicted, and are consistent with recently isolated MTB strains. It suggested that the anoxic metabolic network might be one restricted constraint for MTB distribution in bacterial lineages. Interestingly, analyses from ribosomal proteins to the whole MTB genome strongly support a taxonomic chimeric nature of MO-1 and MC-1 genes, and may represent a novel Proteobacteria lineage. Additionally, I also participate to genome analyses of piezophilic Desulfovibrio and Phaeospirillum molischianum strains as well as genome-Wide analysis of bacterial tyrosine kinases
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Pronger, II Gregory Emery. "Convergent Validity Between the Questions About Behavioral Function (QABF) Questionnaire, Trial-Based Functional Analysis, and Traditional Functional Analysis for Adults with a Dual Diagnosis in a Day Program Setting." OpenSIUC, 2015. https://opensiuc.lib.siu.edu/theses/1705.
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Previous research has demonstrated that individuals with a dual diagnosis often engage in challenging behavior as a means to fulfil their needs and wants. Functional behavioral assessments (FBA) are a way of evaluating these behaviors and creating effective interventions to reduce them and increase socially appropriate alternative behaviors. The present study assessed the convergent validity for three types of FBAs, including the Questions About Behavioral Function (QABF) questionnaire, trial-based functional analysis, and traditional functional analysis, for three adults with a dual diagnosis within a day program setting. Results found correspondence between two forms of assessments, the trial-based functional analysis and traditional functional analysis, for one out of the three subjects. Due to a lack of engagement in the targeted behavior for the other two subjects, results were inconclusive. Results of the QABF did not match those of the functional analyses for any of the subjects, suggesting that the assessment should be used with caution. The trial-based functional analysis may be a viable tool for assessing function for the challenging behavior of adults with a dual diagnosis, although it should not be used as a replacement for the traditional functional analysis.
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Rachman, Helmy. "Functional genome analysis of Mycobacterium tuberculosis." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=970869746.
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Swiatek, Magdalena. "Functional analysis of plastid-encoded genes." Diss., lmu, 2002. http://nbn-resolving.de/urn:nbn:de:bvb:19-1680.
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Lee, Ho-Jin. "Functional data analysis: classification and regression." Texas A&M University, 2004. http://hdl.handle.net/1969.1/2805.
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Functional data refer to data which consist of observed functions or curves evaluated
at a finite subset of some interval. In this dissertation, we discuss statistical
analysis, especially classification and regression when data are available in function
forms. Due to the nature of functional data, one considers function spaces in presenting
such type of data, and each functional observation is viewed as a realization
generated by a random mechanism in the spaces. The classification procedure in
this dissertation is based on dimension reduction techniques of the spaces. One commonly
used method is Functional Principal Component Analysis (Functional PCA) in
which eigen decomposition of the covariance function is employed to find the highest
variability along which the data have in the function space. The reduced space of
functions spanned by a few eigenfunctions are thought of as a space where most of the
features of the functional data are contained. We also propose a functional regression
model for scalar responses. Infinite dimensionality of the spaces for a predictor causes
many problems, and one such problem is that there are infinitely many solutions. The
space of the parameter function is restricted to Sobolev-Hilbert spaces and the loss
function, so called, e-insensitive loss function is utilized. As a robust technique of
function estimation, we present a way to find a function that has at most e deviation
from the observed values and at the same time is as smooth as possible.
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Zoglat, Abdelhak. "Analysis of variance for functional data." Thesis, University of Ottawa (Canada), 1994. http://hdl.handle.net/10393/10136.
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In this dissertation we present an extension to the well known theory of multivariate analysis of variance. In various situations data are continuous stochastic functions of time or space. The speed of pollutants diffusing through a river, the real amplitude of a signal received from a broadcasting satellite, or the hydraulic conductivity rates at a given region are examples of such processes. After the mathematical background we develop tools for analyzing such data. Namely, we develop estimators, tests, and confidence sets for the parameters of interest. We extend these results, obtained under the normality assumption, and show that they are still valid if this assumption is relaxed. Some examples of applications of our techniques are given. We also outline how the latter can apply to random and mixed models for continuous data. In the appendix, we give some programs which we use to compute the distributions of some of our tests statistics.
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LeFevre, Kristin. "Teacher-Conducted Trial-Based Functional Analysis." Master's thesis, Temple University Libraries, 2017. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/471189.
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Applied Behavioral AnalysisEd.M.
Problem behavior often occurs in individuals with autism spectrum disorder (ASD). Functional Analysis (FA) is a method used to identify the function of challenging behavior. Preliminary research has demonstrated that results from Trial Based Function Analysis (TBFA) conducted in natural settings often match the results obtained when conducting standard FAs in analogue settings. The effects of a training package on TBFA implementation fidelity were evaluated using a multiple baseline design across teachers. Three teachers in a special education setting were trained to implement the TBFA during role-plays and classroom probes with the students. All three teachers maintained high fidelity across each condition and over time. The results of which are then used to developed an individualized function-based treatment plan to examine the effects of the plan on reducing problem behavior of one individual with ASD. Results indicated positive effects of the function-based intervention on decreasing challenging behavior. Keywords: functional analysis, trial-based functional analysis, Autism Spectrum Disorder, problem behavior
Temple University--Theses
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Plaza-Menacho, Ivan. "Functional analysis of RET in MEN2." [S.l. : [Groningen : s.n.] ; University Library Groningen] [Host], 2006. http://irs.ub.rug.nl/ppn/293101930.
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Friman, Ola. "Adaptive analysis of functional MRI data /." Linköping : Univ, 2003. http://www.bibl.liu.se/liupubl/disp/disp2003/tek836s.pdf.
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Crimmins, Stephen Lewis. "Characterization and functional analysis of Usp14." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. https://www.mhsl.uab.edu/dt/2007p/crimmins.pdf.
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Stahl, Ronny. "Identification and functional analysis of Trnp1." Diss., lmu, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-154329.
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Cheng, Jia. "Functional analysis of MAPK phosphatase AtMKP2." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/7297.
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Plants have evolved complex signal transduction pathways to sense and respond to the fast-changing environment. Among several crucial signaling pathways, MAPK pathways are known to be involved in regulating many biological processes, including development, cytokinesis, biotic and abiotic stress signaling and hormone signaling. As negative regulators of MAP kinases (MPKs), MAPK phosphatases (AtMKPs) can reverse the activation status of AtMPKs by dephosphorylating the activation sites of AtMPKs. There are 5 putative AtMKPs in the Arabidopsis genome and previous research has shown they play an important role in MAP kinase control. AtMKP2 has been shown to be a novel regulator for ozone stress responses, but how it might be involved in other biological aspects of Arabidopsis development and growth remains unknown. In this study, I examined the biological functions of AtMKP2 in trichome development. Phenotype analysis showed that in AtMKP2-RNAi mutants, trichome density as well as trichome branching number were affected. Both proAtMKP2: GUS signal and proAtMKP2:YFP signal showed that AtMKP2 was expressed in all development stages of developing trichomes. RT-PCR showed that the expression levels of several known trichome development regulators were affected in AtMKP2-RNAi plants. Genetic analysis of AtMKP2 RNAi x try and AtMKP2 RNAi x cpc double mutants showed phenotype consistent with the involvement of AtMKP2 in trichome development. To characterize the biological processes in which AtMKP2 plays a role, I also employed microarray approaches to examine the short-term transcriptional events in AtMKP2 LOF and GOF mutants. I was able to validate the microarray data of AtMKP2 LOF mutants using qRT-PCR. However, the gene expression patterns in AtMKP2 GOF mutants were not verified. This might result from the different overexpression levels of AtMKP2 in different biological replicates. Several defense-related genes showed transcriptional changes in both AtMKP2 LOF and GOF mutants, suggesting AtMKP2 might function in response to pathogen attack. In all, I studied the biological function of AtMKP2 in Arabidopsis trichome development and placed AtMKP2 within the network of known trichome development regulators. My microarray experiments provided some useful clues suggesting other biological functions of AtMKP2.
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Engelman, William R. "A functional analysis of multiple movements." Diss., Georgia Institute of Technology, 1987. http://hdl.handle.net/1853/28924.
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Townsley, Fiona M. "Functional analysis of the KDEL receptor." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360610.
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Sawers, Ruairidh J. H. "Functional analysis of bundle sheath defective2." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342541.
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Dhitavat, Jittima. "Functional analysis of Darier's disease gene." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270363.
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White, Sharon. "Kindler Syndrome - Molecular and Functional Analysis." Thesis, University of Dundee, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500628.
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Mitchell, Neil. "Transformation and analysis of functional programs." Thesis, University of York, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.495901.
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Barry, E. R. "Functional analysis of Sulfolobus solfataricus MCM." Thesis, University of Cambridge, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.596432.
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The minichromosome maintenance (MCM) complex is thought to function as the replicative helicase in eukaryotes and archaea. It is a member of the AAA+ (ATPases associated with various cellular activities) protein family. In this thesis, I demonstrated that the isolated AAA+ domain of Sulfolobus solfataricus MCM is a functional helicase, however its catalytic properties are very different to those of the full length protein. The N-terminus of the protein is required to confer substrate specificity on the enzyme, and also to enhance processive helicase activity. Communication between DNA binding sites in the N- and C-termini of the protein is shown to depend on a highly conserved loop, which protrudes from the N-terminus of the protein towards the AAA+ domain, where it interacts with a DNA binding β-hairpin in a dynamic, nucleotide dependent manner. This communication is mediated between, rather than within, allowing a model to be proposed for how helicase activity is co-ordinated; both between DNA binding sites within subunits, but also between subunits around the ring. Finally, I investigated reports of phosphorylated forms of MCM in vivo and identify a phosphorylated serine residue in the AAA+ domains of endogenous S. solfataricus MCM. Mutant forms of the protein with phosphomimetics at this site have elevated helicase activity in vitro. To identify the kinase responsible, several S. solfataricus kinases are investigated biochemically, and one is identified which has high levels of kinase activity on MCM. This is shown to interact with MCM in vivo and to be regulated in a cell cycle dependent manner.
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Lawry, Robert G. "Evolutionary analysis of mastrevirus functional regions." Thesis, University of Canterbury. School of Biological Sciences, 2010. http://hdl.handle.net/10092/4715.
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New and emerging virus species are becoming an increasing threat to our way of life economically and physically. Plant viruses are particularly significant as they affect our food supply and are capable of rapidly spreading to new plant species. Geminiviruses are a group of viruses that highlight this phenomenon well. Indeed Geminiviruses are some of the earliest recorded plant viruses being described as far back as 752 AD in a Japanese poem written to describe geminivirus symptoms in eupatorium leaves (Saunders et al.,2003). More recently, and in a more threatening manner, Geminiviruses have adapted to infect key crop species such as maize, sugarcane, tomatoes, beet and many more. An example of this is the introduction of grasses such as Maize into Africa, which allowed a
species jump for mastreviruses, which were endemic in native grasses (Varsani et al.,2008a). Over a relatively short period of evolutionary time a number of new
Geminiviruses have emerged, making them a good model for understanding the evolution and spread of new plant pathogens. The economic importance of Geminiviruses also
makes an understanding of their mechanisms of adaptation crucial in preventing new emergence and minimising the impact of current strains.
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El-Hassi, Mohamed F. "Functional analysis of some yeast genes." Thesis, Durham University, 1997. http://etheses.dur.ac.uk/4767/.
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A series of mutant strains of the yeast Saccharomyces cerevisiae that are sensitive to osmotic stress and also have a defect in vacuolar biogenesis have been isolated (M. Latterich, PhD Thesis 1992). The mutations that cause this pleiotropic phenotype are termed ssv, for salt sensitive vacuolar mutants. Complementation analysis has revealed that ssv mutations fall into one of 18 complementation groups. A MAP kinase related signal transduction pathway, termed the HOG pathway for High Osmolarity Glycerol, has been identified in yeast. This pathway senses osmotic stress and invokes the cellular response, one aspect of which is the accumulation of intracellular glycerol (Brewster et. al, 1993). Mutations in the HOG pathway often cause an osmosensitive phenotype similar to that shown by ssv mutations. This work sets out to characterise several ssv strains for defects in the HOG pathway. These strains were subjected to osmotic stress and the intracellular and extracellular glycerol determined and compared to control strains and conditions. Many of the strains showed reduced, or even elevated in one case, glycerol levels compared to wild-type strains. No correlation could be made between these glycerol levels and the activity of the rate-limiting enzyme, glycerol-3-phosphate dehydrogenase (GPDH) determined in an independent study. Transcription of the GPDH gene is under the control of the HOG pathway. In a separate study, the nucleotide sequence of a short region of yeast chromosome VII was determined. Approximately 11,000 bases of DNA from the right sub-telomeric region was sequenced. Analysis of the DNA sequence showed four potential open reading frames. One of these encoded the YORl gene and another a protein related to PAU1 The remaining two ORFs, termed ORFl and ORF2, encoded potential proteins of unknown function. Disruption cassettes containing the LEU2 selectable marker were constructed for both ORFl and ORF2. Successful disruption of ORFl was achieved, but no viable transformants were ever recovered after attempted disruption of 0RF2..ORFl gene knockouts are viable and show no observable phenotype under a range of growth conditions. Subsequent analysis of ORFl and 0RF2 after the completion of the Yeast Genome Project, shows that both ORFl and 0RF2 are members of different sub- telomeric associated gene families. 0RF2 encodes a putative Y' protein.
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Hansen, Soren Prag. "Structural and functional analysis of muskelin." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.408222.
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