Relevant bibliographies by topics / Frozen thawed bull seman

Academic literature on the topic 'Frozen thawed bull seman'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Contents

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Frozen thawed bull seman.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Frozen thawed bull seman"

1

Turk, G., M. Yuksel, M. Sonmez, S. Gur, S. Ozer Kaya, and E. Demirci. "Effects of semen sexing kits (HeiferplusTM and BullplusTM) supplemented to frozen-thawed bull semen on pregnancy rates, foetal sex ratios and selected reproductive parameters in cows." Veterinární Medicína 60, No. 6 (July 15, 2016): 309–13. http://dx.doi.org/10.17221/8245-vetmed.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Ntemka, A., G. Tsousis, C. Brozos, E. Kiossis, CM Boscos, and IA Tsakmakidis. "Breed differences of bull frozen-thawed semen." Reproduction in Domestic Animals 51, no. 6 (September 25, 2016): 945–52. http://dx.doi.org/10.1111/rda.12769.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Morató, Roser, Noelia Prieto-Martínez, Rodrigo Muiño, Carlos O. Hidalgo, Joan E. Rodríguez-Gil, Sergi Bonet, and Marc Yeste. "Aquaporin 11 is related to cryotolerance and fertilising ability of frozen–thawed bull spermatozoa." Reproduction, Fertility and Development 30, no. 8 (2018): 1099. http://dx.doi.org/10.1071/rd17340.

Full text
Abstract:
Aquaporins (AQPs) are channel proteins involved in the transport of water and solutes across biological membranes. In the present study we identified and localised aquaporin 11 (AQP11) in bull spermatozoa and investigated the relationship between the relative AQP11 content, sperm cryotolerance and the fertilising ability of frozen–thawed semen. Bull ejaculates were classified into two groups of good and poor freezability and assessed through immunofluorescence and immunoblotting analyses before and after cryopreservation. AQP11 was localised throughout the entire tail and along the sperm head. These findings were confirmed through immunoblotting, which showed a specific band of approximately 50 kDa corresponding to AQP11. The relative amount of AQP11 was significantly (P < 0.05) higher in both fresh and frozen–thawed spermatozoa from bull ejaculates with good freezability compared with those with poorer freezability. In addition, in vitro oocyte penetration rates and non-return rates 56 days after AI were correlated with the relative AQP11 content in fresh spermatozoa. In conclusion, AQP11 is present in the head and tail of bull spermatozoa and its relative amount in fresh and frozen–thawed spermatozoa is related to the resilience of the spermatozoa to withstand cryopreservation and the fertilising ability of frozen–thawed spermatozoa. Further research is needed to elucidate the actual role of sperm AQP11 in bovine fertility.
APA, Harvard, Vancouver, ISO, and other styles
4

Siqueira, L. G. B., C. W. Palmer, and C. Lessard. "222 THE USE OF IN VITRO FERTILIZATION TO STUDY BOVINE IDIOPATHIC INFERTILITY." Reproduction, Fertility and Development 21, no. 1 (2009): 209. http://dx.doi.org/10.1071/rdv21n1ab222.

Full text
Abstract:
A 4-year-old beef bull underwent a breeding soundness evaluation at the Western College of Veterinary Medicine (Veterinary Hospital, University of Saskatchewan); no apparent abnormalities were observed after conventional semen evaluations. However, the clinical history of this bull indicated that no pregnancies resulted from natural service of 52 cycling cows over a period of 2 years. Completed services were observed. The objective of this study was to use in vitro fertilization (IVF) technology to evaluate whether the sperm from this infertile bull could successfully fertilize oocytes in vitro. Fresh semen was collected with an electroejaculator and frozen in a computer-controlled rate freezer. Concentration and motility parameters were assessed by using computer-assisted semen analyses. Sperm morphology was evaluated on eosin-nigrosin-stained slides, and Coomassie blue staining was used to evaluate the presence of intact acrosomes. Within each evaluation technique, frozen–thawed semen from bulls (n = 2) with proven fertility was used as a positive control and samples of dead sperm (produced by repeated frozen–thawed cycles until reaching no sperm motility) were used as a negative control. Frozen semen from the infertile bull was used for IVF assay. Data were analyzed by ANOVA (sperm motility and the presence of acrosomes) or the chi-square test (cleavage and blastocyst rates), with a P-value of 0.05. Our infertile bull showed an average motile sperm percentage of 91.7 ± 2.2%, with 78.6 ± 3.5% progressive motility. After cryopreservation procedures, frozen–thawed semen had an acceptable general and progressive percentage of motility of 57.8 ± 6.7% and 43.9 ± 9.2%, respectively. Sperm stained with Coomassie blue showed a greater proportion of intact acrosomes in the fresh semen (63.6 ± 4.3% v. 40.4 ± 3.7%; P < 0.05); however, frozen–thawed semen from both the fertile bull and the control were similar (40.4 ± 3.7b% v. 45.5 ± 2.2b%, P < 0.05). In vitro fertilization results revealed a low cleavage rate at 48 h postfertilization (19.8 ± 6.3%) and blastocyst rate (2.4 ± 2.8%) when using frozen–thawed semen from the infertile bull, compared with the control (56.7 ± 8.2% and 26.3 ± 4.5%, respectively; P < 0.001). Moreover, cleavage and blastocyst rates obtained by using the negative control (21.1 ± 3.2% and 1.1 ± 1.9%, respectively) were similar to those of the infertile bull (P > 0.10). It was noted that ova fertilized with either frozen–thawed semen from the infertile bull or the negative control did not produce blastocysts before Days 8 and 9 of embryo culture, which is a characteristic of parthenogenesis. The results from this IVF study suggest that in this bull, there was a missing or defective factor blocking one of the steps in the fertilization process. Further investigations to identify this factor will increase our knowledge of male fertility, and could lead to new methods of evaluating and regulating fertilizing ability.
APA, Harvard, Vancouver, ISO, and other styles
5

Mphaphathi, M. L., M. M. Seshoka, T. R. Netshirovha, Z. C. Raphalalani, T. C. Chokoe, M. Nkadimeng, N. L. Kanuya, J. P. C. Greyling, and T. L. Nedambale. "37 DOUBLE FREEZING AND THAWING OF NGUNI BULL SEMEN." Reproduction, Fertility and Development 28, no. 2 (2016): 148. http://dx.doi.org/10.1071/rdv28n2ab37.

Full text
Abstract:
Indigenous bulls semen are important for conservation programs. The objectives of this study were to evaluate the effects of repeated freezing and thawing on sperm motility characteristics. Semen was collected from 4 Nguni bulls by means of electro ejaculator and stored in a thermo flask (37°C). Sperm total motility, progressive and nonprogressive motility, and velocity were assessed using computer-aided sperm analysis before and after freezing. Semen was then diluted with egg yolk citrate extender (fraction A), then followed by 12% of glycerol + egg yolk citrate extender (fraction B, Seshoka et al. 2012). Diluted semen samples were equilibrated for 4 h at 5°C. After the equilibration period, samples were loaded into 0.25-mL straws and transferred into a controlled rate programmable freezer. After the target temperature of –130°C was reached, semen straws were stored in a LN tank (–196°C). After 3 months of storage, straws were thawed at 15°C (first and second freezing and thawing followed the same process) for 5 min and further evaluated post-thawed at 0 and 15 min during incubation at 15°C. Treatment means were separated using Fisher’s protected t-test least. No significant differences were recorded between the raw semen total sperm motility percentage (93.2%) and first frozen-thawed at 0 min (82.6%), with the total sperm motility rate recovery of 88.6%. In addition, there was a marked decline recorded in sperm total motility during the first frozen-thawed at 15 min (77.6%), second frozen-thawed at 0 min (31.3%), and second frozen-thawed at 15 min (30.1%; P < 0.05). The sperm curvilinear velocity and average path velocity was reduced following first frozen-thawed (P < 0.05) but remained constant and stable between the treatment groups (P > 0.05). In conclusion, the freezing-thawing process did not reduce the Nguni bull total sperm motility during the first freezing and thawing process, compared with raw semen. However, a drastic decline was recorded during the second freezing-thawing processes, compared with raw semen.
APA, Harvard, Vancouver, ISO, and other styles
6

Arat, S., S. Pabuccuoglu, H. Sagirkaya, K. Demir, R. Arici, B. Ustuner, S. Alcay, et al. "22 SEMEN AND REPRODUCTIVE PROFILES OF CLONED ANATOLIAN GREY CATTLE." Reproduction, Fertility and Development 27, no. 1 (2015): 103. http://dx.doi.org/10.1071/rdv27n1ab22.

Full text
Abstract:
Anatolian grey cattle (endangered native Anatolian cattle) as 1 male (clone 1) and 4 females (clones 2–5) were produced from cells of 1 male and 1 female cattle by somatic cell nuclear transfer (SCNT) in a previous study. In this study, we examined the reproductive potential of these cloned animals, which are now 4 and 5 years old. The parameters evaluated by phase contrast microscopy for motility, TUNEL for DNA fragmentation, eosin staining for viability, Hoechst 33258 staining and hypo-osmotic swelling test (HOST) for membrane integrity, and fluorescein isothiocyanate-Pisum sativum agglutinin (FITC-PSA) for acrosome integrity of frozen-thawed spermatozoa, as well as birth and survival of calves following insemination with frozen-thawed semen of cloned and nuclear donor bull and normal bull. Six ejaculates and 3 samples per ejaculate from each bull were tested, and the Mann-Whitney U test was used to analyse the data. The spermatological parameters of cloned bull semen – volume, concentration, and motility of fresh – were within accepted limits for artificial insemination (4.60 ± 0.47 mL, 1.55 ± 0.21 × 109 spermatozoa mL–1, 80.00 ± 1.07%, respectively). Frozen-thawed sperm motility and viability rate were higher in the cloned bull (56.6%, 56.7%) than in its nuclear donor (47%, 43%; P < 0.05). Intact membrane and DNA fragmentation rate of cloned bull and its nuclear donor bull sperm were similar (P > 0.05) but the intact acrosome rate of cloned bull was higher than that of its nuclear donor (P < 0.05). Low rates in frozen-thawed sperm of nuclear donor can be related to storage time of sperm which were frozen 5 years before. One (clone 4) of the cloned grey heifers was artificially inseminated with frozen semen from nuclear donor bull and the other (clone 5) was naturally mated with a Holstein bull. Two healthy calves were delivered naturally. When same cloned cows (clones 4–5) and 2 other cloned heifers (clones 2–3) were artificially inseminated with frozen semen of the cloned grey bull, clones 2 and 4 gave birth to 2 healthy female calves. One cloned cow (clone 3) aborted in the third month of gestation and other one (clone 5) is currently 8 months pregnant. Two calves of clone 4 and 5 are 17 months old and 2 other calves of clone 2 and 4 are now 6 and 1 months old. Except for clone 3, our results show that cloned Anatolian grey bull and cows produced from frozen cells in gene bank have normal fertility.
APA, Harvard, Vancouver, ISO, and other styles
7

Malcolm, V., M. Marfil, M. Calvi, F. Rigali, M. Pugliese, J. Gutierrez, M. Panarace, and M. Medina. "365 COMPARISON OF IN VITRO FERTILIZING CAPACITY OF FROZEN - THAWED SEX-SORTED AND SEX-SORTED FROZEN - THAWED BULL SPERMATOZOA." Reproduction, Fertility and Development 19, no. 1 (2007): 298. http://dx.doi.org/10.1071/rdv19n1ab365.

Full text
Abstract:
Sperm sexing has become a world-wide technology, now available in many countries. The method has been incorporated into many reproductive technologies such as embryo production (Zhang et al. 2003 Theriogenology 60, 1657–1663), but sex-sorting is limited when bulls are located far from sorters or when only frozen semen is available. Previous studies on sexing frozen–thawed spermatozoa have been done in rams, which resulted in retention of the spermatozoan functional capacities (Hollinshead et al. 2004 Reproduction 127, 557–568). In vitro characteristics were assessed in bulls after sexing of thawed sperm (Hollinshead et al. 2004 Theriogenology 62, 958–968); however, the fertilizing capacity of frozen–thawed sex-sorted (FTSS) spermatozoa was not tested. The aim of the present study was to compare cleavage and embryo development rate among frozen–thawed (FT), sex-sorted frozen–thawed (SSFT), and FTSS bull spermatozoa. For FT, sperm were diluted to a final concentration of 60 × 106 sperm/mL, packaged in 0.5-mL straws, and frozen. In SSFT, spermatozoa were sex-sorted by flow cytometry following Schenk protocols (1999 Theriogenology 52, 1375–1391). Three × 106 spermatozoa were packaged into 0.25-mL straws and frozen. The final treatment (FTSS) consisted of thawing 6 to 10 frozen straws of 4 different bulls containing an average of 25 × 106 spermatozoa and centrifuging at 600g for 15 min at 21°C to extract cryodiluent. Spermatozoa were diluted and stained with Hoechst 33342 (stain concentration of 112.5 µM, the same used for SSFT treatment) following Schenk sexed-semen protocols (1999), sex-sorted by a flow cytometer, and collected in Tris-base extender containing 20% egg yolk. For each ejaculate frozen–thawed, SSFT and FTSS spermatozoa were prepared for oocyte in vitro fertilization. Also, semen from a bull routinely used as a control in the laboratory was added for a better comparison of results. Oocytes from a slaughterhouse were processed following standard in vitro fertilization procedures (Ferré 2002 Theriogenology 57, 664) 4 times for each bull, and comparison was made between treatments. Results were analyzed by ANOVA. No significant differences were observed among bulls (data not shown) (P &gt; 0.05). Although embryo development rate was statistically different between sexed and non-sexed groups (P &lt; 0.05), results showed that frozen–thawed bull spermatozoa can be sex-sorted and used for in vitro fertilization with comparable developmental rates comparable to those when frozen sexed semen is used (Table 1). This opens a new commercial window for cases where pre-selected sexed embryos from bulls that are not in AI centers are desired, also giving an opportunity for dead bulls. Nevertheless, since a large number of straws are necessary, further studies must be carried out to make this procedure more efficient and economically profitable. Table 1. Results of cleavage and embryo development rates between spermatozoa treatments
APA, Harvard, Vancouver, ISO, and other styles
8

Underwood, S. L., R. Bathgate, D. C. Pereira, A. Castro, P. C. Thomson, W. M. C. Maxwell, and G. Evans. "Embryo production after in vitro fertilization with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm." Theriogenology 73, no. 1 (January 2010): 97–102. http://dx.doi.org/10.1016/j.theriogenology.2009.08.005.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Öztürk, Caner, Şükrü Güngör, Mehmet Bozkurt Ataman, Mustafa Numan Bucak, Nuri Başpinar, Pınar Ili, and Muhammed Enes Inanç. "Effects of arginine and trehalose on post-thawed bovine sperm quality." Acta Veterinaria Hungarica 65, no. 3 (September 2017): 429–39. http://dx.doi.org/10.1556/004.2017.040.

Full text
Abstract:
The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 °C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 ± 8.21%), CASA motility (12.2 ± 5.69%) and progressive motility (3.52 ± 2.13%), compared with the controls (43 ± 2.73%, 55.4 ± 6.78% and 33.48 ± 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 ± 3.99% and 44.1 ± 2.18%) compared with the control (13 ± 8.15 and 31.7 ± 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm.
APA, Harvard, Vancouver, ISO, and other styles
10

Kaka, Asmatullah, Wahid Haron, Rosnina Yusoff, Nurhusien Yimer, A. M. Khumran, Kazhal Sarsaifi, Atique Ahmed Behan, Ubedullah Kaka, Akeel Ahmed Memon, and Mahdi Ebrahimi. "Effect of docosahexanoic acid on quality of frozen–thawed bull semen in BioXcell extender." Reproduction, Fertility and Development 29, no. 3 (2017): 490. http://dx.doi.org/10.1071/rd15089.

Full text
Abstract:
This study was conducted to investigate the effect of docosahexanoic acid (DHA) supplementation in BioXcell extender on the quality of frozen–thawed bull semen. Twenty-four ejaculates were collected from three bulls (eight from each bull). Ejaculates with motility ≥70% and normal morphology ≥80% were extended into BioXcell extender to which 0 (control), 3, 5, 10 or 15 ng mL–1 DHA was added. The supplemented semen samples were incubated at 37°C for 15 min for DHA uptake by spermatozoa. Later, samples were cooled for 2 h at 5°C and packaged into 0.25-mL straws, frozen in liquid nitrogen for 24 h and subsequently thawed for evaluation. Results are presented as percentages ± s.e.m. Supplementation with DHA at 3 ng mL–1 significantly improved sperm functional parameters including sperm motility, normal morphology, viability, acrosome integrity and membrane integrity when compared with other supplemented groups and the control. Lipid peroxidation increased as the incorporation of DHA supplementation increased. In conclusion, 3 ng mL–1 concentration of DHA resulted in superior quality of frozen–thawed bull spermatozoa and is suggested as the optimum level of DHA to be added into BioXcell extender.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Frozen thawed bull seman"

1

Botha, Alma Ester. "Effect of the acidic buffer 2-[n-morpholino] ethanesulfonic acid on frozen-thawed bull semen." Pretoria : [s.n.], 2010. http://upetd.up.ac.za/thesis/available/etd-02252010-141250/.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Botha, Alma Ester. "Effect of the acidic buffer 2-(N-Morpholino) ethanesulfonic acid on frozen-thawed bull semen." Diss., University of Pretoria, 2009. http://hdl.handle.net/2263/22848.

Full text
Abstract:
The aim of the current study was to determine if frozen-thawed bull semen can be treated with the acidic buffer MES (2-[N- morpholino] ethanesulfonic acid) without any detrimental effect on the motility, plasma membrane, acrosomal membrane and longevity of sperm. Frozen bull semen was obtained from a local co-operative. The semen was frozen in 0.25 mL French straws at a concentration of 80 x 106 sperm cells per millilitre. Semen of two different batches from ten bulls of four different breeds was used in this study. Three frozen semen straws of each batch were thawed at 38° C for 25 seconds. The thawed semen was pooled and then split into two aliquots. The one aliquot was used as control, whilst the other was exposed to MES treatment. The motility, plasma membrane integrity, acrosomal membrane integrity and longevity of sperm were evaluated. The effect of MES on motility was minimal as only the percentage of aberrantly motile sperm increased two hours after treatment. Although no effect on the plasma membranes were observed, it can be assumed that some damage did occur due to the fact that the acrosomal membranes were affected significantly. No significant effect was found for longevity of sperm between the control and treated samples, but a significant effect was found for both the control and treated samples over time. Although the detrimental effects caused by MES treatment would render some sperm unable to fertilise an oocyte, it is likely that a sufficient portion of sperm would survive the treatment. It is probable that this treatment would also be effective in frozen-thawed buffalo semen. The following step would be to treat semen of footand-mouth disease positive bulls with MES to establish if treatment with MES will be effective in inactivating foot-and-mouth disease virus in semen of infected bulls. Copyright
Dissertation (MSc (Veterinary Science))--University of Pretoria, 2008.
Production Animal Studies
unrestricted
APA, Harvard, Vancouver, ISO, and other styles
3

Hallap, Triin. "Assessment of sperm attributes of frozen-thawed AI doses from Swedish and Estonian dairy bull sires : with special reference to pre-selection through swim-up, and the influence of age on potential fertility /." Uppsala : Dept. of Clinical Sciences, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/2005113.pdf.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Zhang, Bing Rong. "Evaluation of frozen-thawed semen from Swedish Red and White AI bulls : with special reference to the relation between zona pellucida binding, in vitro fertilization and in vivo fertility /." Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 1998. http://epsilon.slu.se/avh/1998/91-576-5436-0.gif.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Books on the topic "Frozen thawed bull seman"

1

Zhang, Bing Rong. Evaluation of frozen-thawed semen from Swedish red and white AI bulls: With special reference to the relation between zona pellucida binding, in vitro fertilization and in vivo fertility. Uppsala: Sveriges Lantbruksuniversitet, 1998.

Find full text
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Frozen thawed bull seman"

1

Ramírez-Reveco, Alfredo, Jorge Luis Hernández, and Pablo Aros. "Long‐Term Storing of Frozen Semen at −196°C does not Affect the Post-Thaw Sperm Quality of Bull Semen." In Cryopreservation in Eukaryotes. InTech, 2016. http://dx.doi.org/10.5772/64948.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Frozen thawed bull seman' for particular source types:
Journal articles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography