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Academic literature on the topic 'FRET experiments'

Author: Grafiati

Published: 6 September 2023

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Journal articles on the topic "FRET experiments"

1

Kong, Xiangxu, Eyal Nir, Kambiz Hamadani, and Shimon Weiss. "Photobleaching Pathways in Single-Molecule FRET Experiments." Journal of the American Chemical Society 129, no. 15 (April 2007): 4643–54. http://dx.doi.org/10.1021/ja068002s.

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Skruzny, Pohl, and Abella. "FRET Microscopy in Yeast." Biosensors 9, no. 4 (October 11, 2019): 122. http://dx.doi.org/10.3390/bios9040122.

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Förster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo. Moreover, many biochemical and biophysical processes can be followed by employing sophisticated FRET biosensors directly in living cells. Here, we summarize existing FRET experiments and biosensors applied in yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, two important models of fundamental biomedical research and efficient platforms for analyses of bioactive molecules. We aim to provide a practical guide on suitable FRET techniques, fluorescent proteins, and experimental setups available for successful FRET experiments in yeasts.

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Chirio-Lebrun, Maria-Chantal, and Michel Prats. "Fluorescence resonance energy transfer (FRET): theory and experiments." Biochemical Education 26, no. 4 (October 1998): 320–23. http://dx.doi.org/10.1016/s0307-4412(98)80010-1.

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Buning, Ruth, and John van Noort. "Single-pair FRET experiments on nucleosome conformational dynamics." Biochimie 92, no. 12 (December 2010): 1729–40. http://dx.doi.org/10.1016/j.biochi.2010.08.010.

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Hohng, Sungchul, Sanghwa Lee, Jinwoo Lee, and Myung Hyun Jo. "Maximizing information content of single-molecule FRET experiments: multi-color FRET and FRET combined with force or torque." Chem. Soc. Rev. 43, no. 4 (2014): 1007–13. http://dx.doi.org/10.1039/c3cs60184f.

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Barth, Anders, Oleg Opanasyuk, Thomas-Otavio Peulen, Suren Felekyan, Stanislav Kalinin, Hugo Sanabria, and Claus A. M. Seidel. "Unraveling multi-state molecular dynamics in single-molecule FRET experiments. I. Theory of FRET-lines." Journal of Chemical Physics 156, no. 14 (April 14, 2022): 141501. http://dx.doi.org/10.1063/5.0089134.

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Conformational dynamics of biomolecules are of fundamental importance for their function. Single-molecule studies of Förster Resonance Energy Transfer (smFRET) between a tethered donor and acceptor dye pair are a powerful tool to investigate the structure and dynamics of labeled molecules. However, capturing and quantifying conformational dynamics in intensity-based smFRET experiments remains challenging when the dynamics occur on the sub-millisecond timescale. The method of multiparameter fluorescence detection addresses this challenge by simultaneously registering fluorescence intensities and lifetimes of the donor and acceptor. Together, two FRET observables, the donor fluorescence lifetime τD and the intensity-based FRET efficiency E, inform on the width of the FRET efficiency distribution as a characteristic fingerprint for conformational dynamics. We present a general framework for analyzing dynamics that relates average fluorescence lifetimes and intensities in two-dimensional burst frequency histograms. We present parametric relations of these observables for interpreting the location of FRET populations in E–τ D diagrams, called FRET-lines. To facilitate the analysis of complex exchange equilibria, FRET-lines serve as reference curves for a graphical interpretation of experimental data to (i) identify conformational states, (ii) resolve their dynamic connectivity, (iii) compare different kinetic models, and (iv) infer polymer properties of unfolded or intrinsically disordered proteins. For a simplified graphical analysis of complex kinetic networks, we derive a moment-based representation of the experimental data that decouples the motion of the fluorescence labels from the conformational dynamics of the biomolecule. Importantly, FRET-lines facilitate exploring complex dynamic models via easily computed experimental observables. We provide extensive computational tools to facilitate applying FRET-lines.

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Hartmann, Andreas, Frederic Berndt, Simon Ollmann, Georg Krainer, and Michael Schlierf. "In situ temperature monitoring in single-molecule FRET experiments." Journal of Chemical Physics 148, no. 12 (March 28, 2018): 123330. http://dx.doi.org/10.1063/1.5008966.

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Weiss, A., N. Melamed-Book, O. Avital, and M. Brandeis. "A Mixed Cell Protocol for Sensitized Emission FRET Experiments." Microscopy and Microanalysis 12, S02 (July 31, 2006): 434–35. http://dx.doi.org/10.1017/s1431927606062556.

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Schröder, G. F., and H. Grubmüller. "FRETsg: Biomolecular structure model building from multiple FRET experiments." Computer Physics Communications 158, no. 3 (April 2004): 150–57. http://dx.doi.org/10.1016/j.cpc.2004.02.001.

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Hanke, Christian A., Mykola Dimura, Thomas-Otavio Peulen, Holger Gohlke, and Claus A. M. Seidel. "Integrative Molecular Modelling of Biomolecules Guided by FRET Experiments." Biophysical Journal 114, no. 3 (February 2018): 681a. http://dx.doi.org/10.1016/j.bpj.2017.11.3673.

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Dissertations / Theses on the topic "FRET experiments"

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Kallis, Eleni [Verfasser]. "Quantitative single-molecule FRET and its application in experiments on DNA damage recognition by PARP-1 / Eleni Kallis." Ulm : Universität Ulm, 2020. http://d-nb.info/1221671170/34.

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Götz, Markus [Verfasser], Thorsten [Akademischer Betreuer] Hugel, Thorsten [Gutachter] Hugel, and Friedrich [Gutachter] Simmel. "Multi-Color Single-Molecule FRET Experiments and Analysis : The Dynamic Protein System Hsp90 / Markus Götz ; Gutachter: Thorsten Hugel, Friedrich Simmel ; Betreuer: Thorsten Hugel." München : Universitätsbibliothek der TU München, 2017. http://d-nb.info/1172880077/34.

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Koestner, Peter E., Karen Koestner, Daniel G. Neary, and Carl C. Trettin. "An Overview of the Sierra Ancha Experimental Forest's Role in the Free-Air CO₂ Enrichment Large Wood Decomposition Experiment." Arizona-Nevada Academy of Science, 2012. http://hdl.handle.net/10150/301295.

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Minh, David. "Free energy reconstruction from irreversible single-molecule pulling experiments." Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2007. http://wwwlib.umi.com/cr/ucsd/fullcit?p3258785.

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Thesis (Ph. D.)--University of California, San Diego, 2007.
Title from first page of PDF file (viewed June 8, 2007). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 69-72).

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D'Agostino, Silvia. "Particle segregation and mixing in a dry free surface granular flow." Doctoral thesis, Università degli studi di Trento, 2021. http://hdl.handle.net/11572/317018.

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Geophysical massive flows as snow avalanches and debris flows are characterized by a wide grain size distribution. The interactions between particles among the granulometric classes are a consequent of a such large distribution. However, most of the studies on the geophysical massive flows assume the simplifying hypothesis of a constant granulometry. The aim of this work is studying the coexistence of two granulometric classes in statistically stationary and homogeneous conditions and investigating the physical processes responsible for the particles transfer. Experimental investigations using two different grain size classes were conducted to reproduce the two-phases mixture. The granular material was recirculated in a close system and the experiments were carried out in a laboratory flume having a loose two size granular bed. A proper optical technique was innovatively improved to obtain the average and instantaneous values of the velocity and concentration from the side-walls. Through these values, the average particle profiles of velocity, concentration and granular temperature of the two solid fractions were computed. Moreover, the instantaneous values provide the average profiles of the second order correlation of the variables, such as the component of the granular temperature and the fluctuating components of the velocity and the concentration. The average distribution profiles in time of velocity and concentration prove the statistically stationary and homogeneous condition in a stretch sufficiently long of the running flow. Moreover, two types of regimes were identified: in case of low and intermediate discharges, the frictional regime nearly prevails at the free surface; for high discharges there is a coexistence across the flow depth of two regimes, the frictional regime in the intermediate flow-depth region and the collisional regime in the free surface. The existence of a vertical velocity component of both phases brings to hypothesize the presence of secondary circulations in the flow.

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D'Agostino, Silvia. "Particle segregation and mixing in a dry free surface granular flow." Doctoral thesis, Università degli studi di Trento, 2021. http://hdl.handle.net/11572/317018.

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Geophysical massive flows as snow avalanches and debris flows are characterized by a wide grain size distribution. The interactions between particles among the granulometric classes are a consequent of a such large distribution. However, most of the studies on the geophysical massive flows assume the simplifying hypothesis of a constant granulometry. The aim of this work is studying the coexistence of two granulometric classes in statistically stationary and homogeneous conditions and investigating the physical processes responsible for the particles transfer. Experimental investigations using two different grain size classes were conducted to reproduce the two-phases mixture. The granular material was recirculated in a close system and the experiments were carried out in a laboratory flume having a loose two size granular bed. A proper optical technique was innovatively improved to obtain the average and instantaneous values of the velocity and concentration from the side-walls. Through these values, the average particle profiles of velocity, concentration and granular temperature of the two solid fractions were computed. Moreover, the instantaneous values provide the average profiles of the second order correlation of the variables, such as the component of the granular temperature and the fluctuating components of the velocity and the concentration. The average distribution profiles in time of velocity and concentration prove the statistically stationary and homogeneous condition in a stretch sufficiently long of the running flow. Moreover, two types of regimes were identified: in case of low and intermediate discharges, the frictional regime nearly prevails at the free surface; for high discharges there is a coexistence across the flow depth of two regimes, the frictional regime in the intermediate flow-depth region and the collisional regime in the free surface. The existence of a vertical velocity component of both phases brings to hypothesize the presence of secondary circulations in the flow.

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Perreca, Antonio. "Experimental demonstration of displacement noise free interferometry." Thesis, University of Birmingham, 2010. http://etheses.bham.ac.uk//id/eprint/817/.

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General relativity describes gravity as the curvature of space-time. The theory predicts the existence of gravitational waves (GWs), which can be described as ripples in space-time propagating at the speed of light. So far no direct detection of GWs has been achieved. The sensitivities of the currently leading laser interferometric GW detectors are limited by various noise sources, i.e. seismic, thermal, shot noises etc. Several conceptual studies are underway investigating new techniques that aim to improve sensitivities enough to fulfil the requirements of the next generation of detectors. One of these new techniques under investigation is displacement and frequency noise free interferometry (DFI). This thesis reports on the experimental demonstration of a new method of partial DFI that is effective in the GW detection frequency band. The isolation of a mimicked GW signal from displacement noise of one mirror is demonstrated for a detuned Fabry-Perot cavity. A significant reduction in the displacement noise of the cavity input mirror was achieved by properly combining the reflected and transmitted signals from the cavity. This result represents the first experimental demonstration of this recently proposed DFI scheme, and lays the foundations for future work aimed at implementing DFI schemes in up-coming laser interferometric GW detectors.

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Anzuola, Valencia Esdras. "Atmospheric compensation experiments on free-space optical coherent communication systems." Doctoral thesis, Universitat Politècnica de Catalunya, 2015. http://hdl.handle.net/10803/308498.

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In the last years free-space optical communications systems for wireless links have been proposed, studied, and implemented mainly due to the higher bandwidth that this technology is able to provide. Still, radio frequency (RF) systems have been maintained in practical wireless communications systems due to the improvement of the microwave sources and the development of high speed electronics. Nowadays the circumstances are changing as a consequence of the increasing data-rate needed in terrestrial and outer space communications. The shift from RF systems to optical communication systems in the free space applications provide a wide set of advantageous characteristics that are motivating the use of these optical technologies in detriment of the RF systems. One of the key reasons is the advantage of working with optical wavelengths in compare to the RF spectral band. As well as the already mentioned increase in the available bandwidth due to the fact that higher optical frequencies directly mean wider bandwidths, the use of optical frequencies lead to a better performance in terms of the received power: for equal antenna sizes the received signal goes inversely as the square of the wavelength. Of the most interest, recent coherent optical communication systems address modulation and detection techniques for high spectral efficiency and robustness against transmission impairments. Coherent detection is an advanced detection technique for achieving high spectral efficiency and maximizing power or signal-to-noise (SNR) efficiency, as symbol decisions are made using the in-phase and quadrature signals, allowing information to be encoded in all the available degrees of freedom. In this context, the effects of Earth's atmosphere must be taken into account. Turbulenceinduced wavefront distortions affect the transmitted beam responsible for deterioration of the link bit error rate (BER). The use of adaptive optics to mitigate turbulence-induced phase fluctuations in links employing coherent (synchronous) detection is poised to reduce performance penalties enabling a more capable next generation of free-space optical communications. In this work, we describe the implementation of a free space optical coherent communication system using QPSK modulation and heterodyne downconvertion that uses adaptive optics techniques and digital signal processing to mitigate turbulenceinduced phase fluctuations and channel impairments in coherent receivers. A new method for generating atmospheric turbulence based on binary computer generated holography (BCGH) using binary arrays is presented and its performance is evaluated. The feasibility of FSO coherent systems working with adaptive optics is demonstrated and the system performance in terms of the BER is experimentally evaluated under the influence of atmospheric turbulence. The resulting system performance is compared against the theoretical models. The viability of the approach to improve the system efficiency and sensitivity of coherent receivers is experimentally demonstrated.
En los últimos años las comunicaciones ópticas en el espacio libre han sido propuestas, analizadas e implementadas debido, principalmente, al gran ancho de banda disponible mediante esta tecnología. Aún así, en la práctica, los sistemas de radiofrecuencia (RF) han sido mantenidos en las aplicaciones comerciales debido a la mejora de los dispositivos utilizados y al desarrollo de equipos electrónicos con gran velocidad de procesado. Hoy en día la situación está cambiando como consecuencia de un incremento en la tasa de transmisión requerida en sistemas de comunicaciones terrestres y en el espacio exterior. El cambio de sistemas de RF hacia sistemas ópticos en el espacio libre implica una serie de ventajas clave que motiva la transición hacia estas tecnologías. La primera y gran ventaja de trabajar con frecuencias pertenecientes al espectro óptico es el aumento del ancho de banda disponible, ya que trabajar a alta frecuencia implica directamente un incremento en el ancho de banda. Además, la eficiencia en términos de potencia es incrementada, ya que, para un tamaño de antena fijo, la potencia de señal recivida es proporcional al inverso de la longitud de onda al cuadrado. De especial interés es el desarrollo de sistemas de comunicaciones ópticos que utilicen modulaciones complejas, lo que implica una mayor eficiencia espectral y una mayor robustez contra efectos perniciosos introducidos por el canal. La detección coherente es una avanzada técnica que permite un aumento en la eficiencia espectral y maximiza la eficiencia de la potencia recibida. Esto es debido a que los simbolos son demodulados utilizando las señales en fase y cuadratura, aumentando los grados de libertad del sistema. En este contexto, los efectos de la atmósfera sobre las comunicaciones ópticas coherentes deben ser analizadas en detalle. Las turbulencias atmosféricas distorsionan el frente de onda y son responsables del deterioro de la tasa de error en las comunicaciones ópticas en el espacio libre. El uso de óptica adaptativa para mitigar los efectos de turbulencia atmosphérica abre una ventana a la implementación de la próxima generación de sistemas de comunicaciones, basados en tecnologías coherentes. En este trabajo se describe la implementación de un sistema completo de comunicaciones ópticas coherentes utilizando una modulación coherente (QPSK) y detección heterodina. Un sistema de óptica adaptativa y algoritmos de procesado de señal son implementados con el objetivo de mitigar los diferentes efectos introducidos por el canal. Por otro lado, un nuevo método para generar frentes de onda distorsionados por el canal atmosférico es desarrollado y su eficiencia es analizada. Este método se basa en el uso de holografía binaria generada por computador (BCGH) junto con un dispositivo de modulación óptica binaria de bajo coste (DLP). El funcionamiento del sistema completo es verificado y su eficiencia, en términos de tasa de error, son analizados. La eficiencia obtenida experimentalmente es comparada contra los modelos teóricos propuestos en la literatura. La viabilidad del uso de óptica adaptativa para mitigar efectos en sistemas ópticos coherentes es experimentalmente demostrada.

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Callas, Eleni. "How Free Am I?: Where Neuroscientific Experiments Can Lead Philosophy." Thesis, Boston College, 2016. http://hdl.handle.net/2345/bc-ir:106849.

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Thesis advisor: Daniel McKaughan
Thesis advisor: Liane Young
The first two-thirds of this project is an in-depth analysis of the contemporary Free Will debate as it revolves around the Libet et al, Soon et al, and Wegner et al (“Helper Hands”) experiments. The last section of the thesis illustrates in detail the following suggestions regarding the future of the Free Will debate: that there be a shift in the fundamental question of the debate, a shift in the analysis of famous neuroscientific experiments, and a shift in the formation of future experiments that test potential elements of free will
Thesis (BA) — Boston College, 2016
Submitted to: Boston College. College of Arts and Sciences
Discipline: Scholar of the College
Discipline: Philosophy

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Deutschländer, Robert. "Alltagsintuitionen zur Willensfreiheit." Doctoral thesis, Humboldt-Universität zu Berlin, 2018. http://dx.doi.org/10.18452/19245.

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Die Willensfreiheit ist ein zentraler Bestandteil des alltäglichen menschlichen Denkens und bildet eine wichtige Grundlage für Mechanismen unserer Gesellschaft. Trotz dieser zentralen Stellung herrscht unter Philosophen und Psychologen Uneinigkeit darüber, was Willensfreiheit eigentlich bedeutet. Dies wird besonders bei Experimenten zur Untersuchung der Willensfreiheit wie das Libet-Experiment deutlich. In dieser Arbeit wird in drei Surveys empirisch untersucht, ob der Freiheitsbegriff, mit dem die Libet-Experimente operieren, von den Freiheitsintuitionen der Laien gestützt wird, oder ob Laien eher den konträren Freiheitsintuitionen der Philosophen zuneigen. Die Ergebnisse der vorgestellten Untersuchungen zeigen, dass Laien eine von den philosophischen Vorstellungen abweichende Vorstellung von Freiheit haben.
Free will is one of the most crucial concepts in our daily life. It represents one of the most important aspects of daily human behaviour and has crucial importance in societal mechanism. However, despite its importance and long tradition philosophers still disagree on a definition. At the heart of the problem lie diverging intuitions about what is important for the concept of freedom. This is particular obvious in neuroscientific experiment, like the famous Libet-Experiment. Here I investigate in three empirical surveys whether the lay intuitions about freedom match freedom intuitions on which the Libet-experiment are based or rather the classical philosophical intuitions about freedom. For this purpose I adopt the empirical approach of experimental philosophy. The results demonstrate that lay people’s intuitions are pretty much in line with assumption that are made by the Libet-Experiment and contradict common philosophical intuitions about free will.

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Books on the topic "FRET experiments"

1

McLennan, Melanie. 501 TV-free science experiments for kids. Dingley, Vic: Hinkler Books, 2005.

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Konrad Frey: Haus Zankel : Experiment Solararchitektur. Berlin: Jovis, 2013.

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Free Cell. San Francisco, USA: City Lights Books, 2009.

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Surface scattering experiments with conduction electrons. Berlin: Springer-Verlag, 1993.

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Leuthold, Jane H. A free rider experiment for the large class. [Urbana, Ill.]: College of Commerce and Business Administration, University of Illinois at Urbana-Champaign, 1992.

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Kidel, Mark. Beyond the classroom: Dartington's experiments in education. Hartland: Green Books, 1990.

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Edney, Edward. Validation of OH radical reaction rate constant test protocol. Research Triangle Park, NC: U.S. Environmental Protection Agency, Atmospheric Sciences Research Laboratory, 1986.

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DeBruin, Jerry. Creative, hands-on science experiences using free and inexpensive materials. Torrance, CA: Good Apple, 1986.

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Institute, Economic Policy, ed. The failed experiment: NAFTA at three years. [Washington, D.C.]: The Institute, 1997.

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Polites, Michael E. Rotating-unbalanced-mass devices for scanning balloon-borne experiments, free-flying spacecraft, and space shuttle/space station experiments. Huntsville, Ala: George C. Marshall Space Flight Center, 1990.

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Book chapters on the topic "FRET experiments"

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Koopmans, Wiepke J. A., Ruth Buning, and John van Noort. "Engineering Mononucleosomes for Single-Pair FRET Experiments." In DNA Nanotechnology, 291–303. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-142-0_20.

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Gopich, Irina V., and Hoi Sung Chung. "Theory and Analysis of Single-Molecule FRET Experiments." In Protein Folding, 247–82. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1716-8_14.

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Bell, David, Jean-François Doussin, and Thorsten Hohaus. "Preparation of Simulation Chambers for Experiments." In A Practical Guide to Atmospheric Simulation Chambers, 113–27. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-22277-1_3.

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AbstractWhen setting up a simulation chamber experiment it is essential, in order to ensure meaningful results, to start with a well-controlled chemical system. Coming after the chapter dealing with the requested careful characterization of the simulation chamber, the present chapter describes the preparation of the chamber before running an experiment. It includes various chamber cleaning protocols, the preparation of a clean chamber atmosphere (the reacting mixture) and a series of protocols for blank experiments. Indeed, having a clean atmosphere in a simulation chamber, as free as possible from both particulate and gaseous impurities, is essential to ensure high quality experimental results. As it may not be possible to have a perfectly clean chamber, blank experiments are crucial to both assess chamber cleanliness, account for impurities and establish uncertainties of the observed phenomena. In the present chapter, various cleaning protocols which involve the oxidation of the impurities, dilution, temperature degradation/evaporation, but the evacuation or manual cleaning are described as well. The various techniques to generate clean gas mixture—mostly clean O2, N2 or water vapor, are discussed. Finally, complementarily to the reference experiments proposed in Chap. 2, blank experiments to characterize walls chemical inertia, chamber-dependent radical sources or the presence of water-soluble species are also described.

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Rogers, Katherin A. "The Libet Experiments." In The Experimental Approach to Free Will, 61–78. New York: Routledge, 2022. http://dx.doi.org/10.4324/9781003258988-4.

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Brau, Charles A. "Free-Electron Laser Experiments." In Physics of New Laser Sources, 235–46. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4757-6187-0_17.

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Stapp, Henry P. "The Libet “Free Will” Experiments." In Quantum Theory and Free Will, 61–64. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-58301-3_9.

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Glicksman, Martin E., Afina Lupulescu, and Matthew B. Koss. "Quasi-static Melting of Crystals: Experiments and Analysis." In Free Boundary Problems, 167–75. Basel: Birkhäuser Basel, 2003. http://dx.doi.org/10.1007/978-3-0348-7893-7_13.

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Zieliński, Tomasz, Johnny Hay, and Andrew J. Millar. "Period Estimation and Rhythm Detection in Timeseries Data Using BioDare2, the Free, Online, Community Resource." In Methods in Molecular Biology, 15–32. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1912-4_2.

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AbstractOne of the key objectives of data analysis in circadian research is to quantify the rhythmic properties of the experimental data. BioDare2 is a free, online service which provides fast timeseries analysis, attractive visualizations, and data sharing. This chapter outlines the description of an experiment for BioDare2 and how to upload and analyze the numerical timeseries data.

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Holder, Damaris, and Matthias Prigge. "Spatial and Temporal Considerations of Optogenetic Tools in an All-Optical Single-Beam Experiment." In Neuromethods, 165–85. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2764-8_6.

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AbstractAll-optical experiments promise neuroscientists an unprecedented possibility to manipulate and measure neuronal circuits with single-cell resolution. They rely on highly fine-tuned microscopes with complex optical designs. Of similar importance are genetically encoded optical actuators and indicators that also have to be optimized for such experiments. A particular challenge in these experiments is the detection of natural firing patterns via genetically encoded indicators while avoiding optical cross-activation of neurons that are photon-sensitized to allow optical replay of these patterns. Most optogenetic tools are sensitive in a broad spectral range within the visible spectrum, which impedes artifact-free read-and-write access to neuronal circuits. Nonetheless, carefully matching biophysical properties of actuators and indicators can permit unambiguous excitation with a single wavelength in a so-called single-beam all-optical experiment.In this chapter, we evaluate the current understanding of these biological probes and describe the possibilities and limitations of those tools in the context of the all-optical single-beam experiment. Furthermore, we review new insights into the photophysical properties of actuators, and propose a new strategy for a single-beam two-photon excitation experiment to monitor activity minimizing cross-activation with the actuators. Finally, we will highlight aspects for future developments of these tools.

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Khachatryan, Arbak, Artur Tevosyan, David Novoselskiy, Gevorg Arakelyan, Alexey Yushkevich, and David Nazaretovich Nazarian. "Experimental Free Flap Autotransplantation." In Microsurgery Manual for Medical Students and Residents, 197–200. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-73531-9_31.

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Conference papers on the topic "FRET experiments"

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ZENKEVICH, E., T. BLAUDECK, D. KOWERKO, C. VON BORCZYSKOWSKI, and F. CICHOS. "FRET AND NON-FRET PROCESSES IN NANOASSEMBLIES «QUANTUM DOT-ORGANIC MOLECULE» (BULK EXPERIMENTS AND DETECTION OF SINGLE OBJECTS)." In Proceedings of International Conference Nanomeeting – 2011. WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814343909_0044.

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Panzeri, Francesco, Antonino Ingargiola, Ron R. Lin, Niusha Sarkhosh, Angelo Gulinatti, Ivan Rech, Massimo Ghioni, Sergio Cova, Shimon Weiss, and Xavier Michalet. "Single-molecule FRET experiments with a red-enhanced custom technology SPAD." In SPIE BiOS, edited by Jörg Enderlein, Ingo Gregor, Zygmunt K. Gryczynski, Rainer Erdmann, and Felix Koberling. SPIE, 2013. http://dx.doi.org/10.1117/12.2003187.

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"Modeling of single-molecule FRET-experiments on protein folding: from coarse-grained to all-atom simulations." In Bioinformatics of Genome Regulation and Structure/ Systems Biology. institute of cytology and genetics siberian branch of the russian academy of science, Novosibirsk State University, 2020. http://dx.doi.org/10.18699/bgrs/sb-2020-376.

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Wissmann, F. "Quasi-free Compton Scattering from the Deuteron." In Theory and Experiment III. WORLD SCIENTIFIC, 2001. http://dx.doi.org/10.1142/9789812810977_0097.

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Roy, Pascale, and Roger J. Bartlett. "Impact of VUV/Soft X-Ray Free Electron Lasers on the Study of Electron Correlation in Ions." In Free-Electron Laser Applications in the Ultraviolet. Washington, D.C.: Optica Publishing Group, 1988. http://dx.doi.org/10.1364/fel.1988.sa3.

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The study of photoionization of isolated ions, because of its experimental difficulties has lagged that of neutrals even though ions are far more abundant in nature. Here we propose experiments to study the importance of electron correlation- the effect of the motion of one electron on that of all the others- in ions. Correlation effects are often examplified by multiple ionization and/or excitation in photoionization processes. The initial experiment described here consists of measuring the cross sections for single and multiple photoionization of several species. Because of the low density targets and the need for high resolution, the spectral brilliance of a Free Electron Laser (FEL) will be required.

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Rose, Todd S., Darren W. Rowen, Christopher M. Coffman, G. Kinum, Stephen D. LaLumondiere, Nicolette I. Werner, Josef M. Wicker, Geoffrey A. Maul, and Richard P. Welle. "Optical downlink and intersatellite illumination experiments with low-earth orbiting CubeSats." In Free-Space Laser Communications XXXII, edited by Hamid Hemmati and Don M. Boroson. SPIE, 2020. http://dx.doi.org/10.1117/12.2554958.

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Kolev, Dimitar R., Koichi Shiratama, Alberto Carrasco-Casado, Yoshihiko Saito, Yasushi Munemasa, Junichi Nakazono, Phuc V. Trinh, et al. "Preparation of high-speed optical feeder link experiments with “HICALI” payload." In Free-Space Laser Communications XXXIV, edited by Hamid Hemmati and Bryan S. Robinson. SPIE, 2022. http://dx.doi.org/10.1117/12.2610635.

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Hongtao Ma, Yifei Zhang, Zijie Cai, Jeffrey C. Suhling, Pradeep Lall, and Michael J. Bozack. "Aging induced evolution of free solder material behavior." In Multi-Physics simulation and Experiments in Microelectronics. IEEE, 2008. http://dx.doi.org/10.1109/esime.2008.4525093.

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Poh, Edith S. W., W. H. Zhu, X. R. Zhang, C. K. Wang, Anthony Y. S. Sun, and H. B. Tan. "Lead-free solder material characterization for thermo-mechanical modeling." In Multi-Physics simulation and Experiments in Microelectronics. IEEE, 2008. http://dx.doi.org/10.1109/esime.2008.4525046.

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Israel, David J., Bernard L. Edwards, Richard L. Butler, John D. Moores, Sabino Piazzolla, Nic du Toit, and Lena Braatz. "Early results from NASA’s laser communications relay demonstration (LCRD) experiment program." In Free-Space Laser Communications XXXV, edited by Hamid Hemmati and Bryan S. Robinson. SPIE, 2023. http://dx.doi.org/10.1117/12.2655481.

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Reports on the topic "FRET experiments"

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Keefer, Philip, and Razvan Vlaicu. Voting Age, Information Experiments, and Political Engagement: Evidence from a General Election. Inter-American Development Bank, January 2023. http://dx.doi.org/10.18235/0004648.

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We exploit new experimental and quasi-experimental data to investigate voters' intrinsic motivation to engage politically. Does having the right to vote increase engagement or, given significant incentives to free ride, do eligible voters remain rationally unengaged? Does knowledge that ones group is pivotal reduce free riding? And are the politically engaged influenced by election-relevant policy information in the run-up to a major election? To address these questions, we fielded an original survey of 5,400 Mexican high school seniors just prior to the historic 2018 general election. Age-based regression discontinuity results show that the just-eligible score higher on measures of low-cost political engagement compared to the just-ineligible. A first survey experiment reveals that information that the youth vote will be pivotal increases the eligible respondents' interest in the presidential debate and in the election result. In the second experiment, information about current policy outcomes affects future policy priorities in ways consistent with the incentives of eligible respondents to collect relevant information on salient policy issues.

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Wilkins, Justin, Alexandria Barkman, Alexi Meltel, Burton Suedel, and Robert Richmond. Effects of sedimentation on three Hawaiian coral species under laboratory conditions. Engineer Research and Development Center (U.S.), August 2023. http://dx.doi.org/10.21079/11681/47541.

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Sedimentation can occur near a dredge operation in pulses over days, and potentially impact coral reefs occurring in close proximity. To improve the ability to predict the effects of dredging on corals, the effects of sedimentation in two 18-day experiments were studied for three common coral species representing different morphologies. In a laboratory setting, coral fragments were exposed to four sedimentation concentrations dosed every four days ranging from 0 to 60 mg cm-2. Separate experiments were performed in series, once with fine grain sediment and repeated with a coarse grain sediment. A 30-day sediment free observation period followed each experiment. Coral responses were measured throughout the experiment and at the end of the 18-day exposure and 30-day sediment free observation period. Photosynthetic yield, lipid ratios, tissue color, tissue loss, growth, and sediment cover varied among the treatment groups. All coral species were minimally affected when sediment concentrations were at or below 6 mg cm-2. P. meandrina and P. lobata experienced the most sediment coverage and tissue loss when exposed to sediment concentrations >30 mg cm-2 for either sediment. M. capitata experienced no sediment coverage or tissue loss when exposed to either sediment, but a reduction in photosynthetic yield at 60 mg cm-2 fine grain sediment was observed. During the 30-day post-exposure sediment free observation period, P. meandrina tissue loss continued, P. lobata nearly completely regrew lost tissue, while M. capitata showed no lingering effects. This study improves the US Army Corps of Engineers (USACE) ability to estimate the impacts of dredging on coral reefs.

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Molokov, S., and C. B. Reed. Review of free-surface MHD experiments and modeling. Office of Scientific and Technical Information (OSTI), June 2000. http://dx.doi.org/10.2172/757509.

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Gray, Mark Girard. Multigroup Free-atom Doppler-broadening Approximation. Experiment. Office of Scientific and Technical Information (OSTI), November 2015. http://dx.doi.org/10.2172/1225565.

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Nornberg, M. D., H. Ji, J. L. Peterson, and J. R. Rhoads. A Liquid Metal Flume for Free Surface Magnetohydrodynamic Experiments. Office of Scientific and Technical Information (OSTI), August 2008. http://dx.doi.org/10.2172/938980.

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Midak, Lilia Ya, Ivan V. Kravets, Olga V. Kuzyshyn, Jurij D. Pahomov, Victor M. Lutsyshyn, and Aleksandr D. Uchitel. Augmented reality technology within studying natural subjects in primary school. [б. в.], February 2020. http://dx.doi.org/10.31812/123456789/3746.

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The purpose of the research is creation of mobile app (supported by Android) for visualization of chemical structure of water and to display video- data of laboratory experiments that can be used by the teacher and pupils for an effective background for learning natural cycle subjects and performance of laboratory experiments in the elementary school using lapbook. As a result of work, aimed at visualizing the education material, a free mobile app LiCo.STEM was developed; it can be downloaded from the overall-available resource Google Play Market. Representation of the developed video materials on the mobile gadgets is conducted by “binding” them to individual images- “markers” for every laboratory experiment. Applying such technologies gives an opportunity to establish educational activity, based on interference of adults with children, oriented on interests and abilities of each kid, development of curiosity, cognitive motivation and educational energy; development of imagination, creative initiative, including the speech, ability to chose the materials, types of work, participants of the common activity, promotion of conditions for parents participate in the common study activity.

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Concus, P., and R. Finn. Equilibrium liquid free-surface configurations: Mathematical theory and space experiments. Office of Scientific and Technical Information (OSTI), June 1996. http://dx.doi.org/10.2172/281682.

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Wilson, Edward. Experiments in Neural-Network Control of a Free-Flying Space Robot. Fort Belvoir, VA: Defense Technical Information Center, March 1995. http://dx.doi.org/10.21236/ada329618.

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Chen, A., P. Concus, and R. Finn. On cylindrical container sections for a capillary free-surface experiment. Office of Scientific and Technical Information (OSTI), January 1995. http://dx.doi.org/10.2172/34410.

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Fang, Jyan-Min. An inverse free electron laser accelerator: Experiment and theoretical interpretation. Office of Scientific and Technical Information (OSTI), January 1997. http://dx.doi.org/10.2172/484578.

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