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1

MOYLE, PETER B., and MICHAEL P. MARCHETTI. "Predicting Invasion Success: Freshwater Fishes in California as a Model." BioScience 56, no. 6 (2006): 515. http://dx.doi.org/10.1641/0006-3568(2006)56[515:pisffi]2.0.co;2.

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2

Méndez, Oscar, Guillermo Salgado-Maldonado, Juan Manuel Caspetamandujano, and Guillermina Cabañas-Carranza. "Helminth parasites of some freshwater fishes from Baja California Sur, Mexico." Zootaxa 2327, no. 1 (January 4, 2010): 44. http://dx.doi.org/10.11646/zootaxa.2327.1.3.

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Nine freshwater fish species were sampled from 13 bodies of water in Baja California Sur state, on the Baja California peninsula, Mexico. Twenty-four helminth parasite species of 19 genera and from 13 families were recorded. All are new records for the region, but have been recorded previously in Mexico. No endemic helminth species were identified. Most helminths were larval forms of generalist Nearctic species transported by ichthyophagous birds, in addition to five anthropogenically introduced species. Poeciliid fish are the most widely distributed in the oases and other waters of Baja California Sur, and their helminths are the most common on the peninsula.
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3

González, C. J., J. P. Encinas, M. L. Garcı́a-López, and A. Otero. "Characterization and identification of lactic acid bacteria from freshwater fishes." Food Microbiology 17, no. 4 (August 2000): 383–91. http://dx.doi.org/10.1006/fmic.1999.0330.

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4

Bury, Gwendolynn W., Rebecca Flitcroft, Mark D. Nelson, Ivan Arismendi, and Evan B. Brooks. "Forest-Associated Fishes of the Conterminous United States." Water 13, no. 18 (September 15, 2021): 2528. http://dx.doi.org/10.3390/w13182528.

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Freshwaters are important, interconnected, and imperiled. Aquatic ecosystems, including freshwater fishes, are closely tied to the terrestrial ecosystems they are embedded within, yet available spatially explicit datasets have been underutilized to determine associations between freshwater fishes and forested areas. Here, we determined the spatial co-occurrence between freshwater fish distributions and forests within 2129 watersheds of the conterminous United States. We identified 21% of freshwater fishes as associated with forested areas, and 2% as strictly present only in highly forested areas (75–100% forested). The northern coasts and southeast regions, both heavily forested, showed the largest numbers of forest-associated fishes in highly forested areas and fish species richness. Fish associated with low-forested areas occurred in the southwest and central plains. Imperiled fishes were relatively evenly distributed among percent forest categories, which was distinctly different from patterns for all fishes. The identification of forest-associated fishes provides insights regarding species-specific landscape contexts. Determining these large-scale patterns of freshwater biodiversity is necessary for conservation planning at regional levels, especially in highly impacted freshwater ecosystems.
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5

Lagler, Karl F. "Freshwater Fishes of California. California Natural History Guides, Volume 49. Samuel M. McGinnis , Arthur C. Smith." Quarterly Review of Biology 60, no. 4 (December 1985): 530. http://dx.doi.org/10.1086/414657.

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6

Ebner, Brendan C., David L. Morgan, Adam Kerezsy, Scott Hardie, Stephen J. Beatty, Jamie E. Seymour, James A. Donaldson, et al. "Enhancing conservation of Australian freshwater ecosystems: identification of freshwater flagship fishes and relevant target audiences." Fish and Fisheries 17, no. 4 (April 27, 2016): 1134–51. http://dx.doi.org/10.1111/faf.12161.

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7

Dzikowski, R., MG Levy, MF Poore, JR Flowers, and I. Paperna. "Use of rDNA polymorphism for identification of Heterophyidae infecting freshwater fishes." Diseases of Aquatic Organisms 59 (2004): 35–41. http://dx.doi.org/10.3354/dao059035.

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8

Zimik, Philayung, and Bishnupada Roy. "Molecular identification of two cestodes species parasitizing freshwater fishes in India." Journal of Parasitic Diseases 43, no. 1 (December 17, 2018): 59–65. http://dx.doi.org/10.1007/s12639-018-1058-z.

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9

Gobalet, Kenneth W. "Prehistoric Status of Freshwater Fishes of the Pajaro-Salinas River System of California." Copeia 1990, no. 3 (September 19, 1990): 680. http://dx.doi.org/10.2307/1446434.

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10

Angulo Sibaja, Arturo, Alex Molina Arias, Atsunobu Murase, Yusuke Miyazaki, William Albert Bussing, and Myrna Isabel López Sánchez. "Fishes from the Tusubres River basin, Pacific coast, Costa Rica: checklist, identification key and photographic album." Check List 11, no. 3 (May 28, 2015): 1666. http://dx.doi.org/10.15560/11.3.1666.

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A checklist of the fishes of the Tusubres River basin, Pacific coast of Costa Rica, compiled from field and museum surveys is herein presented. A total of 54 species, representing 47 genera and 27 families, were recorded. Peripheral species were dominant (64.8%), followed by secondary freshwater fishes (20.4%); primary freshwater fishes accounted only for 14.8% of the total fish diversity. Eleotridae (6 spp.), Gobiidae (6 spp.), Poeciliidae (5 spp.) and Characidae (4 spp.) were the most diverse. Two species (Caranx sexfasciatus, Carangidae; and Opisthonema libertate, Clupeidae) were new records for Costa Rican freshwaters, and two species (Gymnotus maculosus, Gymnotidae; and Lebiasina boruca, Lebiasinidae) was found to have expanded ranges. An identification key and a complete photographic album of all fish species recorded in the basin are presented. The results of this investigation provide a framework for future studies on biogeography, ecology and conservation on fishes from this area.
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11

Meng, Lesa, and Scott A. Matern. "Native and Introduced Larval Fishes of Suisun Marsh, California: The Effects of Freshwater Flow." Transactions of the American Fisheries Society 130, no. 5 (September 2001): 750–65. http://dx.doi.org/10.1577/1548-8659(2001)130<0750:nailfo>2.0.co;2.

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12

Saiki, Michael K., Mark R. Jennings, and Thomas W. May. "Selenium and other elements in freshwater fishes from the irrigated San Joaquin valley, California." Science of The Total Environment 126, no. 1-2 (September 1992): 109–37. http://dx.doi.org/10.1016/0048-9697(92)90487-d.

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13

Young, Matthew, Emily Howe, Teejay O’Rear, Kathleen Berridge, and Peter Moyle. "Food Web Fuel Differs Across Habitats and Seasons of a Tidal Freshwater Estuary." Estuaries and Coasts 44, no. 1 (July 11, 2020): 286–301. http://dx.doi.org/10.1007/s12237-020-00762-9.

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AbstractEstuarine food webs are fueled by multiple different primary producers. However, identifying the relative importance of each producer to consumers is difficult, particularly for fishes that utilize multiple food sources due to both their mobility and their generally high trophic levels. Previous studies have documented broad spatial differences in the importance of primary producers to fishes within the Upper San Francisco Estuary, California, including separation between pelagic and littoral food webs. In this study, we evaluated the importance of primary producers to adult fishes in three closely spaced subregions that represented disparate habitat types (a tidal wetland channel, a turbid backwater channel, and a deep open-water channel), each a potential outcome of local restoration projects. Using stable isotope analysis coupled with a Bayesian mixing model, we identified significant differences in primary-producer contribution to fishes and invertebrates across habitats and seasons, especially in the relative contribution of submersed aquatic vegetation and phytoplankton. Most fishes utilized multiple primary producers and showed little segregation between pelagic and littoral food webs among habitats. Availability of primary producers differs seasonally and across multiple spatial scales, helping to buffer environmental variability and thus enhancing food web resilience. Ecosystem restoration may improve with emphasis on restoring a wide variety of primary producers to support consumers.
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14

MCMAHAN, CALEB D., WILFREDO A. MATAMOROS, FRANCISCO S. ÁLVAREZ CALDERÓN, WENDY YAMILETH HENRÍQUEZ, H. MICHELLE RECINOS, PROSANTA CHAKRABARTY, ENRIQUE BARRAZA, and NÉSTOR HERRERA. "Checklist of the Inland Fishes of El Salvador." Zootaxa 3608, no. 6 (January 23, 2013): 440–56. http://dx.doi.org/10.11646/zootaxa.3608.6.2.

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The inland fish fauna of El Salvador and its distribution was originally described in 1925 by Samuel Hildebrand. That work has been the main source of information for freshwater fishes of El Salvador up to today. Based on the combination of an intensive literature review, electronic database searches, re-identification of museum specimens, and fieldwork, we hereby provide an updated checklist of the inland fishes of El Salvador. This checklist provides distributional data at the Salvadoran hydrographical and political (by department) levels. The checklist is systematically arranged at the ordinal and familial level and then alphabetically therein. The freshwater fish fauna of El Salvador includes 101 species divided into 64 genera, 29 families, and 14 orders. According to their supposed tolerance to salinity, 73% of these species are peripheral, 23% secondary, and only 4% are primary freshwater fishes. One species is endemic to the country, Amatitlania coatepeque. The low number of primary freshwater fishes and endemics is comparable to the Central American Pacific slope in particular, as well as northern Central America in general.
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15

Angulo, Arturo, Carlos A. Garita-Alvarado, William A. Bussing, and Myrna I. López. "Annotated checklist of the freshwater fishes of continental and insular Costa Rica: additions and nomenclatural revisions." Check List 9, no. 5 (October 1, 2013): 987. http://dx.doi.org/10.15560/9.5.987.

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Based on a combination of intensive literature review, electronic database searches, re-identification of museum specimens and fieldwork, we hereby provide an updated checklist of the freshwater fishes of continental and insular Costa Rica. This checklist, systematically arranged at the ordinal and familial level, includes nomenclatural revisions, distributional information, and when appropriate, cross-references on the basis of Bussing (1998). According to our results, the native Costa Rican freshwater fish fauna is composed by 250 species, divided into 119 genera, 49 families and 19 orders; increasing in 108 the number of species originally reported by Bussing (1998). By far, the vast majority of these species, according to their supposed tolerance to salinity, are peripheral (63.2%), followed by secondary freshwater fishes (23.6%); only 13.2% are primary freshwater fishes. 24 species in this checklist appear to be endemic to Costa Rica. In addition to the native fauna 8 exotic species are reported.
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16

Ahmed, Md Sagir, Sujan Kumar Datta, and Ayesha Akhter Zhilik. "Molecular diversity of freshwater fishes of Bangladesh assessed by DNA barcoding." Bangladesh Journal of Zoology 48, no. 1 (June 29, 2020): 1–19. http://dx.doi.org/10.3329/bjz.v48i1.47872.

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This study represents the comprehensive molecular identification of freshwater fishes of Bangladesh based on a fragment of the cytochrome c oxidase subunit I (COI) gene in the mitochondrial genome. A total of 315 mitochondrial COI barcode sequences were obtained from 153 species,114 genera, 49 families and 16 orders of fishes. The mean length of the sequences was 652 base pairs. For all the samples, %G was significantly lower compared to the other nucleotides and %GC was lower compared to %AT (p-value ˂ 0.05). Also, a significantly lower %GC content was observed in second and third codon position compared to the first one in all the samples (1st>2nd>3rd, p-value˂ 0.05). The average K2P distances within species, genera, families and orders were 0.38%, 7.02%, 12.75% and 18.68%, respectively. The mean interspecific distance was 18-fold higher than the mean intraspecific distance. The K2P neighbor-joining (NJ) trees based on the sequences generally clustered species according to their taxonomic position. A total of 12 species were newly recorded in Bangladesh. High efficiency in species identification were demonstrated in the present study by DNA barcoding, and concluded that COI sequencing can be used as an authentic identification marker for freshwater fish species. Bangladesh J. Zool. 48(1): 1-19, 2020
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17

Alò, Dominique, Cristian Correa, Horacio Samaniego, Corey A. Krabbenhoft, and Thomas F. Turner. "Otolith microchemistry and diadromy in Patagonian river fishes." PeerJ 7 (January 3, 2019): e6149. http://dx.doi.org/10.7717/peerj.6149.

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Coastal habitats in Chile are hypothesized to support a number of diadromous fish species. The objective of this study was to document migratory life histories of native galaxiids and introduced salmonids from a wide latitudinal range in Chilean Patagonia (39–48°S). Otolith microchemistry data were analysed using a recursive partitioning approach to test for diadromy. Based on annular analysis of Sr:Ca ratios, a diadromous life history was suggested for populations of native Aplochiton taeniatus, A. marinus, and Galaxias maculatus. Lifetime residency in freshwater was suggested for populations of A. zebra and G. platei. Among introduced salmonids, populations of Oncorhynchus tshawytscha and O. kisutch exhibited patterns consistent with anadromy, whereas the screened population of O. mykiss appeared restricted to freshwater. Salmo trutta exhibited variable patterns suggesting freshwater residency and possibly anadromy in one case. The capacity and geographic scope of hydropower development is increasing and may disrupt migratory routes of diadromous fishes. Identification of diadromous species is a critical first step for preventing their loss due to hydropower development.
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18

Moyle, Peter B., Joseph D. Kiernan, Patrick K. Crain, and Rebecca M. Quiñones. "Climate Change Vulnerability of Native and Alien Freshwater Fishes of California: A Systematic Assessment Approach." PLoS ONE 8, no. 5 (May 22, 2013): e63883. http://dx.doi.org/10.1371/journal.pone.0063883.

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19

Moyle, Peter B. "Conservation of native freshwater fishes in the Mediterranean-type climate of California, USA: A review." Biological Conservation 72, no. 2 (1995): 271–79. http://dx.doi.org/10.1016/0006-3207(94)00089-9.

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20

WHITE, L. R., B. A. MCPHERON, and J. R. STAUFFER. "Identification of freshwater mussel glochidia on host fishes using restriction fragment length polymorphisms." Molecular Ecology 3, no. 2 (April 1994): 183–85. http://dx.doi.org/10.1111/j.1365-294x.1994.tb00121.x.

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21

Couch, Alan J., Fiona Dyer, and Mark Lintermans. "Multi-year pair-bonding in Murray cod (Maccullochella peelii)." PeerJ 8 (December 10, 2020): e10460. http://dx.doi.org/10.7717/peerj.10460.

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Mating strategies in fishes are known to include polygyny, polyandry and monogamy and provide valuable insights regarding powerful evolutionary forces such as sexual selection. Monogamy is a complex of mating systems that has been relatively neglected. Previous work on mating strategies in fishes has often been based on observation and focused on marine species rather than freshwater fishes. SNPs are increasingly being used as a molecular ecology tool in non-model organisms, and methods of probabilistic genetic analysis of such datasets are becoming available for use in the absence of parental genotypes. This approach can be used to infer mating strategies. The long-term pair bonding seen in mammals, reptiles and birds has not been recorded in freshwater fishes—in every other respect an extremely diverse group. This study shows that multi-year pair bonding occurs in an Australian Percichthyid fish that exhibits paternal care of eggs and larvae. Using SNPs, full sibling pairs of larvae were found over multiple years in a three-year study. Stable isotope signatures of the larvae support the genetic inference that full sibling pairs shared a common mother, the ultimate source of that isotopic signature during oogenesis. Spatial and temporal clustering also suggests that the full sibling larvae are unlikely to be false positive identifications of the probabilistic identification of siblings. For the first time, we show multi-year pair bonding in a wild freshwater fish. This will have important conservation and management implications for the species. This approach could provide insights into many behavioural, ecological and evolutionary questions, particularly if this is not a unique case. Our findings are likely to initiate interest in seeking more examples of monogamy and alternative mating strategies in freshwater fishes, particularly if others improve methods of analysis of SNP data for identification of siblings in the absence of parental genotypes.
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22

Behrens-Chapuis, Simone, Fabian Herder, and Matthias F. Geiger. "Adding DNA barcoding to stream monitoring protocols – What’s the additional value and congruence between morphological and molecular identification approaches?" PLOS ONE 16, no. 1 (January 4, 2021): e0244598. http://dx.doi.org/10.1371/journal.pone.0244598.

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Although aquatic macroinvertebrates and freshwater fishes are important indicators for freshwater quality assessments, the morphological identification to species-level is often impossible and thus especially in many invertebrate taxa not mandatory during Water Framework Directive monitoring, a pragmatism that potentially leads to information loss. Here, we focus on the freshwater fauna of the River Sieg (Germany) to test congruence and additional value in taxa detection and taxonomic resolution of DNA barcoding vs. morphology-based identification in monitoring routines. Prior generated morphological identifications of juvenile fishes and aquatic macroinvertebrates were directly compared to species assignments using the identification engine of the Barcode of Life Data System. In 18% of the invertebrates morphology allowed only assignments to higher systematic entities, but DNA barcoding lead to species-level assignment. Dissimilarities between the two approaches occurred in 7% of the invertebrates and in 1% of the fishes. The 18 fish species were assigned to 20 molecular barcode index numbers, the 104 aquatic invertebrate taxa to 113 molecular entities. Although the cost-benefit analysis of both methods showed that DNA barcoding is still more expensive (5.30–8.60€ per sample) and time consuming (12.5h), the results emphasize the potential to increase taxonomic resolution and gain a more complete profile of biodiversity, especially in invertebrates. The provided reference DNA barcodes help building the foundation for metabarcoding approaches, which provide faster sample processing and more cost-efficient ecological status determination.
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23

Dick, T. A., and B. C. Poole. "Identification of Diphyllobothrium dendriticum and Diphyllobothrium latum from some freshwater fishes of central Canada." Canadian Journal of Zoology 63, no. 1 (January 1, 1985): 196–201. http://dx.doi.org/10.1139/z85-030.

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Two species of Diphyllobothrium occurred in Quigly Lake, Manitoba: plerocercoids of D. dendriticum encapsulated on the viscera of shallow water cisco (Coregonus artedii) and lake whitefish (Coregonus clupeaformis) and plerocercoids of D. latum unencapsulated in the muscle of walleye (Stizostedion vitreum vitreum) and northern pike (Esox lucius). Adult D. dendriticum were obtained through experimental infections of both herring gulls (Larus argentatus) and hamsters (Mesocricetus auratus), but adult D. latum developed experimentally only in the latter. The scolex position of adult D. dendriticum was more anterior than D. latum in the small intestine of hamsters. Eggs were present in utero and in the faeces by days 10–11 postinfection (PI) for D. dendriticum in hamsters and gulls and by day 17 PI for D. latum in hamsters. Adult D. dendriticum grew longer and had more segments in gulls than in hamsters. The neck length of adult D. latum was at least five times greater than the neck length of adult D. dendriticum in hamsters by day 17 PI. Viewed laterally, the seminal vesicle in adult D. dendriticum was dorsal to the cirrus sac, while in D. latum, the seminal vesicle was dorsocaudal to the cirrus sac. A constriction between segments was noted for adult D. latum only.
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24

Manaresi, Sonia, Barbara Mantovani, and Francesco Zaccanti. "Egg to adult identification of 13 freshwater fishes from Italy: a biochemical-genetic key." Aquatic Sciences 63, no. 2 (May 2001): 182–90. http://dx.doi.org/10.1007/pl00001350.

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25

Álvarez, Francisco S., Saúl González Murcia, Caleb D. McMahan, and Wilfredo A. Matamoros. "Cyprinodontiform fishes of El Salvador." UNED Research Journal 13, no. 1 (March 25, 2021): 24. http://dx.doi.org/10.22458/urj.v13i1.3303.

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Introduction: The Cyprinodontiformes are a group of secondary freshwater fishes widely distributed in El Salvador. Currently, many species of this group are usually incorrectly identified for lack of adequate tools. Additionally, their taxonomy and distribution have changed in recent years. Objective: To provide updated information about the taxonomy and distribution of El Salvador Cyprinodontiformes through identification keys, distribution notes, and general descriptions for all species. Methods: We carried out an extensive review of the literature, electronic databases, and museum specimens to generate a list of valid species present in El Salvador. Results: Eleven species in three families are confirmed: Profundulidae (two species), Anablepidae (one species), and Poeciliidae (eight species). We also include distribution data, both vertical and by main basins, and an illustrated guide. Conclusions: There are 11 species of Cyprinodontiformes in El Salvador and they can be found and identified with this article.
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26

ROBERTS, TYSON R. "Francis Hamilton and the freshwater stingrays described in his Gangetic fishes (1822)." Archives of Natural History 25, no. 2 (June 1998): 267–79. http://dx.doi.org/10.3366/anh.1998.25.2.267.

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Direct evidence bearing on identification of the two new species of Gangetic stingrays named Raia fluviatilis and R. sancur by Hamilton, 1822 comprises 1) the first written account by Hamilton (then Buchanan) of his encounters with Gangetic stingrays in 1807–1813, written at the time in manuscript, but not published until 1877; 2) Hamilton's accounts of Raia fluviatilis and Raia sancur published in 1822; 3) Hamilton drawing IV 7 in the archives of the Asiatic Society of Bengal (never published, original now lost); and 4) Hamilton drawing IV 65 in the same archives (published by Hora, 1929, original now lost; this drawing is not a copy or a version of drawing IV 7). The description of R. sancur clearly is based on a species of the genus Pastinachus. Drawing IV 7 presumably is the unfinished drawing of R. sancur mentioned by Hamilton, 1822, and is therefore also of a Pastinachus. Drawing IV 65, not mentioned by Hamilton, a complete drawing with dorsal and ventral views of a newborn male Pastinachus with an intact sting, is identified as based on Raia fluviatilis. Pending revision of the genus Pastinachus, the Gangetic species is tentatively identified as P. sephen (Forsskål, 1775). Identification of Raia fluviatilis with a large freshwater species of Gangetic Himantura advocated by Annandale, 1910; Chaudhuri, 1912; Compagno and Cook, 1996; and Zorzi, 1996 is based on unwarranted assumptions. There is no definite evidence that Hamilton ever saw a Gangetic Himantura. Himantura chaophraya Monkolprasit and Roberts, 1990 is the only available name applicable to huge large tropical Asian freshwater stingrays of the dasyatid genus Himantura. No specimens of Gangetic Himantura exist in present museum collections.
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27

Arisuryanti, Tuty, Rika Lathif Hasan, Khadija Lung Ayu, Nofita Ratman, and Lukman Hakim. "Genetic Identification of Freshwater Fish Species Through DNA Barcoding from Lake Lebo Taliwang, West Nusa Tenggara." Journal of Tropical Biodiversity and Biotechnology 4, no. 3 (December 16, 2019): 107. http://dx.doi.org/10.22146/jtbb.46256.

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Lake Lebo Taliwang is one of the lakes in the West Nusa Tenggara with high freshwater fish species diversity. However, the species identification of freshwater fish species from Lake Lebo Taliwang using DNA barcoding is very limited. Therefore, the objective of this study was to identify seven samples of freshwater fish species collected from Lake Lebo Taliwang based on COI mitochondrial gene as a DNA barcoding marker and establish library COI sequences of Indonesian freshwater fish. We are using a standard DNA analysis and data obtained from this study was then examined using Nucleotide BLAST and the phylogenetic tree was analyzed using the Neighbour-Joining (NJ) method with Kimura 2 Parameter (K2P) model. The results revealed that among the seven samples of freshwater fishes collected from the Lake Lebo Taliwang, three samples were identified as Anabas testudineus, the other three samples were Barbodes binotatus and one sample was Trichopodus trichopterus. The level of similarity of these freshwater fish samples referred to the database from the GenBank and BOLD was between 98-100%. The NJ tree supports the clade of each species identified in this study. This occurrence indicated that DNA barcoding by using the COI mitochondrial gene was proven to be able to identify the freshwater fish samples accurately.
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28

Blake, Tim. "A Field Guide to Freshwater Fishes, Crayfish and Mussels of South-Western Australia." Pacific Conservation Biology 18, no. 2 (2012): 148. http://dx.doi.org/10.1071/pc120148.

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MORGAN et al. have filled a significant gap in the library of pictorial guides to the fauna of South- Western Australia with this publication. This pocketsized edition is a must for anyone who has any interest in the natural history of the South West as well as schools, community groups and anyone who wishes to start investigating the fauna of our rivers. Not only will this guide help anyone with identification, but it will alert them to the very existence of so many native species. Perhaps any small fish will no longer be written off as a “mosquito fish” and elicit a more detailed look. It is quite surprising that there are only 11 native species of fish in the whole south-west province but impressive that 9 are endemic – the highest ratio in any part of Australia. The presence of as many crayfish species (11), many of very limited distribution, is also of note. However, it is quite disturbing that 12 (and likely more) introduced fish species are also listed for identification. A further reflection is that only 1 fish and 3 crayfish in the whole of Australia are on the Critically Endangered list under the Environmental Protection and Biodiversity Conservation Act 1999 – all of these are in the area covered by the guide.
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29

Richardson, John S., Eric Taylor, Dolph Schluter, Mike Pearson, and Todd Hatfield. "Do riparian zones qualify as critical habitat for endangered freshwater fishes?" Canadian Journal of Fisheries and Aquatic Sciences 67, no. 7 (July 2010): 1197–204. http://dx.doi.org/10.1139/f10-063.

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Identification of critical habitat is a key step in conservation and recovery of endangered and threatened freshwater fish. Critical habitat under Canadian and US legislation may include habitat that is not directly used by listed fish, provided it is necessary for species conservation or recovery. Riparian habitat meets biological criteria for critical habitat because riparian zones are integral to aquatic ecosystem functions of importance to many fish species and other organisms. These functions include provision of shade for temperature-sensitive species, control of channel complexity and sediment inputs through bank stabilization, input of large wood and allochthonous energy sources, and filtering of nutrients and toxins from adjacent land. In response to decades of stream-riparian research, widespread implementation of regulations to protect riparian zones in most developed countries represent a de facto consensus that riparian buffers are essential for aquatic ecosystem health and the maintenance of populations of fish and other species. Consistent with widespread riparian regulations deemed necessary to protect not-at-risk species, riparian habitat adjacent to a body of water containing a listed freshwater species should be considered biologically critical unless the habitat requirements of individual taxa are demonstrated to be insensitive to the ecological functions associated with riparian habitat.
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30

Hulthén, K., B. B. Chapman, P. A. Nilsson, L. A. Hansson, C. Skov, H. Baktoft, J. Brodersen, and C. Brönmark. "Sex identification and PIT-tagging: tools and prospects for studying intersexual differences in freshwater fishes." Journal of Fish Biology 84, no. 2 (February 2014): 503–12. http://dx.doi.org/10.1111/jfb.12300.

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31

Bagley, Justin C., Pedro De Podestà Uchôa Aquino, María Florencia Breitman, Francisco Langeani, and Guarino R. Colli. "DNA barcode and minibarcode identification of freshwater fishes from Cerrado headwater streams in Central Brazil." Journal of Fish Biology 95, no. 4 (July 29, 2019): 1046–60. http://dx.doi.org/10.1111/jfb.14098.

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32

Gu, Zemao, Jianguo Wang, Ming Li, Jinyong Zhang, Xiaoli Ke, and Xiaoning Gong. "Morphological and genetic differences of Trypanosoma in some Chinese freshwater fishes: difficulties of species identification." Parasitology Research 101, no. 3 (June 9, 2007): 723–30. http://dx.doi.org/10.1007/s00436-007-0536-5.

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Murwantoko, Murwantoko, Eka Diniarti, and Triyanto Triyanto. "Isolation, Characterization and Pathogenicity of Edwardsiella tarda a Causative Disease on Freshwater Fish in Yogyakarta." Jurnal Perikanan Universitas Gadjah Mada 21, no. 1 (August 2, 2019): 41. http://dx.doi.org/10.22146/jfs.39920.

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Edwarsiella tarda is a cosmopolitan bacterium and is a cause of Edwardsiellosis in various fish species. The bacterial infection causes large losses on aquaculture in Asia, especially Japan. This study was conducted to isolate and characterize E. tarda as causative disease in freshwater fishes, and to determine its pathogenicity to catfish (Pangasius sp.). Bacteria were isolated from kidney of diseased fishes on Tryptone Soya Agar medium. Identification was conducted based on morphological colonies, morphological cells and biochemical tests. Fulfillment of Koch Postulates was done by injecting bacteria intraperitoneally on 7-9 cm fishes at dose of 107 cfu/fish. Pathogenicity test was carried out by intraperitoneal injection at 104, 105, 106, and 107 cfu/fish to 7-9 cm-catfish (Pangasius sp.) and followed by observation of disease signs and mortality every six hours for 7 days. Pathogenicity was determined as Lethal Dosage (LD50) using Dragstedt Behrens method. In this research we have isolated three isolates E. tarda causing disease in fishes. The clinical signs of this disease were lose of pigmentation over the lession, swollen of stomach, haemorhage on fins , small cutaneous lesions, and necrotic on fins area. The LD50 of E. tarda isolate L2, L3, and N3 were 4.64 ± 0.35x105, 1.54 ± 0.07x105, and 1.13 ± 0.13x106 cfu/fish, respectively.
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Evans, Karen L. "Documentation and Identification of the One Known Freshwater Sponge Discovered in the California Delta." Open Marine Biology Journal 4, no. 1 (October 12, 2010): 82–86. http://dx.doi.org/10.2174/1874450801004010082.

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35

ANGULO, ARTURO, JORGE SAN GIL-LEÓN, ALEJANDRO OVIEDO-SOTO, JUAN I. ABARCA-ODIO, and GERARDO UMAÑA-VILLALOBOS. "The fishes from the Coto River basin, Pacific coast, Costa Rica." Zootaxa 4751, no. 1 (March 16, 2020): 1–28. http://dx.doi.org/10.11646/zootaxa.4751.1.1.

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A checklist of the fishes of the Coto River basin, southern Pacific, Costa Rica, compiled from field and museum surveys is presented. A total of 61 species, representing 48 genera, 26 families and 11 orders are listed. Peripheral species were dominant (44.3%), whereas secondary and primary freshwater species represented 32.8% and 23.9% of the total diversity. The orders Cyprinodontiformes (12 spp.) and Perciformes (11) and the families Poeciliidae (8) and Cichlidae (7) were the most diverse. Two species (Lutjanus guttatus and Polydactylus approximans) were new records for Costa Rican freshwaters and ten additional species were found to have expanded geographical ranges. An identification key and a complete photographic album of all fish species recorded are presented. This investigation provides a framework for future studies on fishes from this area complementing previous efforts seeking to increase our knowledge about the freshwater ichthyofauna of the central and southern Pacific regions of Costa Rica.
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Pangle, Kevin L., Stuart A. Ludsin, and Brian J. Fryer. "Otolith microchemistry as a stock identification tool for freshwater fishes: testing its limits in Lake Erie." Canadian Journal of Fisheries and Aquatic Sciences 67, no. 9 (September 2010): 1475–89. http://dx.doi.org/10.1139/f10-076.

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We evaluated otolith chemistry as a tool for identifying natal origins of potamodromous fishes using historical Lake Erie water chemistry (1983–2001) and yellow perch ( Perca flavescens ) otolith elemental composition (1994–1996) data. Lake Erie’s tributaries had stream-specific chemical signatures that were temporally stable. Correspondingly, the otolith microelemental composition of larvae collected from tributary embayments (Sandusky and Maumee bays) was shown to be geographically distinct and the use of known-origin juveniles showed that larval otolith microelemental signatures could be used to accurately identify natal origins and indicate fish movement. Discrimination between offshore spawning locations was relatively difficult, however, indicating limitations to working in systems that are dominated by flow from a single large river (i.e., Detroit River). Interannual variability in otolith microelemental signatures was high such that larvae from one year could not reliably classify natal location of larvae in another year. Development of an annual library of site-specific signatures and exploration of complementary ways to discriminate natal origins would improve the use of otolith microchemistry as a fishery management tool in freshwater systems.
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Day, Julie L., Jennifer L. Jacobs, and Josh Rasmussen. "Considerations for the Propagation and Conservation of Endangered Lake Suckers of the Western United States." Journal of Fish and Wildlife Management 8, no. 1 (March 1, 2017): 301–12. http://dx.doi.org/10.3996/022016-jfwm-011.

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Abstract Decades of persistent natural and anthropogenic threats coupled with competing water needs have compromised numerous species of freshwater fishes, many of which are now artificially propagated in hatcheries. Low survival upon release is common, particularly in systems with substantial nonnative predator populations. Extensive sampling for Shortnose (Chasmistes brevirostris) and Lost River Suckers (Deltistes luxatus) in the Klamath River Basin on the California–Oregon border have failed to detect any new adult recruitment for at least two decades, prompting an investigation into artificial propagation as an extinction prevention measure. A comprehensive assessment of strategies and successes associated with propagation for conservation restocking has not been performed for any Catostomid. Here, we review available literature for all western lake sucker species to inform propagation and recovery efforts for Klamath suckers and summarize the relevance of these considerations to other endangered fishes.
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Wibowo, Arif, Achmad Farajalah, and Husnah Husnah. "DNA BARCODING OF FRESHWATER FISH SPECIES OF MANNA RIVER (BENGKULU) AND SEMANKA RIVER (LAMPUNG)." Indonesian Fisheries Research Journal 19, no. 1 (June 30, 2013): 9. http://dx.doi.org/10.15578/ifrj.19.1.2013.9-17.

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The identification of fish species is challenging. DNA barcoding provides new perspective in ecology and systematics of fishes. In this study, we assess intraspecific and interspecific genetic divergence among several freshwater fish species from Manna River of Bengkulu Province and Semanka River of Lampung Province, using data from mitochondrial cytochrome c oxidase subunit I (COI) gene. The results show that a total of six freshwater fish species identified in this study exhibited high levels of interspecific variation based on 51 analyzed sequences. Almost all species corresponded to a single species, cohesive array of barcode sequences distinct from other species. Based on Kimura Two Parameters (K2P) within species, mean pairwise sequence distances comparisons range from 0.3 to 6.3%, while interspecific comparisons were much higher range between 1.7 to 29.4%, except species Tor macrolepis and Tor putitora showed high levels of genetic similarities and no reciprocal monophyly. Using this method will obviously allow the identification eggs, larvae, fillets and fins of freshwater fish.
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Snyder, Randal J., and Hugh Dingle. "Adaptive, genetically based differences in life history between estuary and freshwater threespine sticklebacks (Gasterosteus aculeatus L.)." Canadian Journal of Zoology 67, no. 10 (October 1, 1989): 2448–54. http://dx.doi.org/10.1139/z89-345.

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Life history theory predicts that migratory fishes should delay reproduction, be larger at maturity, and have higher fecundities than nonmigrants. We tested this hypothesis by comparing life histories of laboratory-reared estuary and freshwater threespine sticklebacks (Gasterosteus aculeatus L.) from the Navarro River, California. We also estimated phenotypic correlations and genetic variance (broad-sense heritabilities) for reproductive characteristics of these fish. The more migratory estuary sticklebacks delayed reproduction, were larger at first reproduction, and had higher fecundities than the freshwater fish. We found no significant differences in interclutch interval or average size of eggs. The body size – fecundity relationship did not differ among these populations, unlike the findings in previous reports comparing anadromous and freshwater threespine sticklebacks. We found significant levels of genetic variance for age and size at first reproduction in both populations, and for fecundity in the freshwater population. The estuary and freshwater sticklebacks did not differ in average number of fin rays, gill rakers, or lateral plates, indicating that differentiation in life history has not been accompanied by significant changes in these characteristics. These results provide evidence of genetic divergence between these populations with respect to life histories, and the nature of these differences suggests that adaptation to different migratory life-styles has occurred.
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Kalous, Lukáš, Denisa Nechanská, and Miloslav Petrtýl. "Survey of angler's internet posts confirmed the occurrence of freshwater fishes of the genus Ictiobus (Rafinesque, 1819) in natural waters of Czechia." Knowledge & Management of Aquatic Ecosystems, no. 419 (2018): 29. http://dx.doi.org/10.1051/kmae/2018019.

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The information regarding the fish species occurrence in rivers and lakes depends on the quantity and quality of ichthyofaunal surveys. Non-native buffalo fishes, Ictiobus cyprinellus and I. niger (Catostomidae) were introduced to pond aquaculture in the 20th century but since that time they have never been recorded from the natural environment in Europe. Forensic analysis was performed using online data from Czech anglers websites to test whether these fishes were ever caught. In total 13 records were obtained but only 3 were verified in terms of species identification, locality and date. The found records are scattered throughout the area of Czechia and they are without an obvious distribution pattern. The analysis of online fishing web sites can be considered a suitable source of additional information on the occurrence of fishes.
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Ude, George N., David O. Igwe, Chrysten Brown, Myron Jackson, Alusine Bangura, Onyinye Ozokonkwo-Alor, Onyinye C. Ihearahu, et al. "DNA barcoding for identification of fish species from freshwater in Enugu and Anambra States of Nigeria." Conservation Genetics Resources 12, no. 4 (June 5, 2020): 643–58. http://dx.doi.org/10.1007/s12686-020-01155-7.

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Abstract Within Enugu and Anambra States, Nigeria, identification of fishes has been based on morphological traits and do not account for existing biodiversity. For DNA barcoding, assessment of biodiversity, conservation and fishery management, 44 fish sampled from Enugu and Anambra States were isolated, amplified and sequenced with mitochondrial cytochrome oxidase subunit I (COI). Twenty groups clustering at 100% bootstrap value including monophyletic ones were identified. The phylogenetic diversity (PD) ranged from 0.0397 (Synodontis obesus) to 0.2147 (Parachanna obscura). The highest percentage of genetic distance based on Kimura 2-parameter was 37.00 ± 0.0400. Intergeneric distances ranged from 15.8000 to 37.0000%. Congeneric distances were 6.9000 ± 0.0140–28.1000 ± 0.0380, with Synodontis as the existing synonymous genus. Confamilial distances in percentage were 16.0000 ± 0.0140 and 25.7000 ± 0.0300. Forty-two haplotypes and haplotype diversity of 0.9990 ± 0.0003 were detected. Nucleotide diversity was 0.7372, while Fu and Li’s D* test statistic was 2.1743 (P < 0.02). Tajima’s D was 0.2424 (P > 0.10) and nucleotide frequencies were C (17.70%), T (29.40%), A (24.82%), G (18.04%) and A + T (54.22%). Transitional mutations were more than transversions. Twenty species (99–100%) were identified with the e-value, maximum coverage and bit-score of 1e−43, 99–100 and 185–1194, respectively. Seventeen genera and 12 families were found and Clariidae (n = 14) was the most dominant among other families. The fish species resolution, diversity assessment and phylogenetic relationships were successfully obtained with the COI marker. Clariidae had the highest number of genera and families. Phylogenetic diversity analysis identified Parachanna obscura as the most evolutionarily divergent one. This study will contribute to fishery management, and conservation of freshwater fishes in Enugu and Anambra States, Nigeria.
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Swieca, K., S. Sponaugle, C. Briseño-Avena, MS Schmid, RD Brodeur, and RK Cowen. "Changing with the tides: fine-scale larval fish prey availability and predation pressure near a tidally modulated river plume." Marine Ecology Progress Series 650 (September 17, 2020): 217–38. http://dx.doi.org/10.3354/meps13367.

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Tidally controlled river plumes form distinct frontal boundaries that can alter the spatial distributions of larval fishes and their planktonic prey and predators. Variable in nature, they may expose larval fishes to different trophic environments over small spatio-temporal scales, with unknown consequences for survival and recruitment. In the northern California Current, the Columbia River Plume is strongly influenced by twice-daily freshwater injections that create a highly dynamic coastal environment. Using the In situ Ichthyoplankton Imaging System, we examined changes in the fine-scale horizontal and vertical distributions of larval fishes, their prey, and their predators over space and time (ebb/flood tide). In total, 6095 fish larvae and ~1.5 million prey/predator zooplankton were imaged and measured. Plume regions provided substantially higher concentrations of prey and enhanced spatial overlap between larval fishes and their prey relative to oceanic waters. The functionality of river plumes as a refuge from predators was less clear. Predator concentrations were also higher in plume regions, but overlap with larval fishes was taxon-specific and varied with the tide. Notably, regions of high zooplankton concentrations did not necessarily confer high spatial overlap on small scales (meters vertical, kms horizontal) relevant to trophic interactions. Surface salinity and chlorophyll a were the most important factors influencing the spatial overlap of zooplankton with larval fishes. In the vicinity of river plumes, larval fishes experience a diversity of unique prey and predator fields over short spatio-temporal scales, which likely contribute to variable growth and mortality patterns at much finer scales than previously thought.
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Xia, Chun, Zhi-Hong Ma, M. Habibur Rahman, and Zhi-Guang Wu. "PCR cloning and identification of the β-haemolysin gene of Aeromonas hydrophila from freshwater fishes in China." Aquaculture 229, no. 1-4 (January 2004): 45–53. http://dx.doi.org/10.1016/s0044-8486(03)00398-3.

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44

Rodriguez-Silva, Rodet, James Josaphat, Patricia Torres-Pineda, and Ingo Schlupp. "Annotated list of livebearing fishes (Cyprinodontiformes: Poeciliinae) from Lake Miragoane in Southwestern Haiti, Hispaniola." Novitates Caribaea, no. 17 (January 18, 2021): 147–62. http://dx.doi.org/10.33800/nc.vi17.249.

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Within the Caribbean region Lake Miragoane in southwestern Haiti represents one of the most important radiation centers of livebearing fishes of the subfamily Poeciliinae. However, there is a lack of scientific studies documenting the distribution, number of species and conservation status of the fishes from that lake. In this work, an annotated list of livebearing fishes, the most well represented group of aquatic vertebrates in Lake Miragoane, is presented with the corresponding species identification and images. This is the first study in the last 40 years to capture most of the fish diversity in the lake originated from the subgenus Limia and especially in the species flock represented by the subgenus Odontolimia. It also includes two recently described species: Limia islai and L. mandibularis. Here we also report some conservation threats to the endemic freshwater fish fauna from Lake Miragoane that should be addressed to preserve the biodiversity in this important area.
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Hardy, Christopher M., Mark Adams, Dean R. Jerry, Leon N. Court, Matthew J. Morgan, and Diana M. Hartley. "DNA barcoding to support conservation: species identification, genetic structure and biogeography of fishes in the Murray - Darling River Basin, Australia." Marine and Freshwater Research 62, no. 8 (2011): 887. http://dx.doi.org/10.1071/mf11027.

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Freshwater fish stocks worldwide are under increasing threat of overfishing, disease, pollution and competition from introduced species. In the Murray—Darling Basin (MDB), the largest river system of Australia, more than half the native species are listed as rare or endangered. Active management is required to counteract reduction in population sizes, prevent local extinctions and to maintain genetic diversity. We describe the first comprehensive set of DNA barcodes able to discriminate between all 58 native and introduced species of freshwater fish recorded in the MDB. These barcodes also distinguish populations from those in adjacent basins, with estimated separation times as short as 0.1 million years ago. We demonstrate the feasibility of using DNA fingerprinting of ribosomal RNA (12S and 18S rRNA) genes and mitochondrial DNA control region (mtDNA CR) sequences to identify species from eggs, larvae, tissues and predator gut contents as well as differentiate populations, morphologically cryptic species and hybrids. The DNA barcode resource will enhance capacity in many areas of fish conservation biology that can benefit from improved knowledge of genetic provenance. These include captive breeding and restocking programs, life history studies and ecological research into the interactions between populations of native and exotic species.
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Gani, Abdul, Achmad Afif Bakri, Devita Tetra Adriany, Nurjirana Nurjirana, Muh Herjayanto, Monicha Indrasari Bungalim, Samliok Ndobe, and Andi Iqbal Burhanuddin. "IDENTIFICATION OF FRESHWATER GOBY SPECIES FROM THE BIAK AND KOYOAN RIVERS, LUWUK BANGGAI, CENTRAL SULAWESI." Jurnal Ilmu Kelautan SPERMONDE 5, no. 2 (January 3, 2020): 57. http://dx.doi.org/10.20956/jiks.v5i2.8931.

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Sulawesi is an island famous for its biodiversity, including many endemic species. In particular, Sulawesi has the highest number of gobies in the world, including species with potential as food and/or ornamental fishes. The exploration of freshwater ichthyofauna is important in the context of Indonesian and global biodiversity. This research aimed to identify gobies found in the Luwuk Banggai area of Central Sulawesi, as a contribution to the exploration of Indonesian ichthyofaunal biodiversity. Gobies were sampled from January to March 2019 in the Biak and Koyoan Rivers. The sampled fish were measured and identified based on morphological characteristics. A total of 52 specimens were collected, and identified as belonging to 17 species within two families, the Gobiidae and Eleotridae. Gobies from the Koyoan River comprised 32 specimens from 8 species, while 20 specimens belonging to species were found in the Biak River. Only two species were found in both rivers: Stiphodon semoni and Sicyopterus lagocephalus. These results augment the body of knowledge regarding the presence and distribution of gobies in Indonesia
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Cheok, Yi Ying, Suat Moi Puah, Kek Heng Chua, and Jin Ai Mary Anne Tan. "Isolation and molecular identification of Aeromonas species from the tank water of ornamental fishes." Acta Veterinaria Hungarica 68, no. 2 (October 13, 2020): 130–39. http://dx.doi.org/10.1556/004.2020.00029.

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AbstractAeromonads are recognised as important pathogens of fishes. In this study, ten water samples were randomly collected from pet shops' fish tanks and home aquaria inhabited by several fish species (silver arowana, koi, goldfish, catfish, pictus fish, silver shark and silver dollar fish). Altogether 298 colonies were isolated using Aeromonas selective agar. A total of 154 isolates were then confirmed as belonging to the genus Aeromonas using the GCAT gene. Using ERIC-PCR, a total of 40 duplicate isolates were excluded from the study and 114 isolates were subjected to PCR-RFLP targeting the RNA polymerase sigma factor (rpoD) gene using lab-on-chip. A total of 13 different Aeromonas species were identified. The most prevalent species were A. veronii (27%, 31/114), followed by A. dhakensis (17%, 19/114), A. finlandiensis (9%, 10/114), A. caviae (8%, 9/114), A. hydrophila (4%, 4/114), A. jandaei (4%, 4/114), A. rivuli (3%, 3/114), A. enteropelogens (2%, 2/114), A. tecta (2%, 2/114), A. allosaccharophila (1%, 1/114), A. eucrenophila (1%, 1/114), A. media (1%, 1/114) and A. diversa (1%, 1/114). Twenty-six isolates (23%) were unidentifiable at species level. The present study demonstrates that Aeromonas species are highly diverse in freshwater fish tanks, and suggests the potential risks posed by the isolated bacteria to the health of ornamental fish species.
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Vaz-dos-Santos, André Martins, and Estevan Luiz da Silveira. "IDADE E CRESCIMENTO DE PEIXES DE RIACHO: MÉTODOS E DESAFIOS PARA A OBTENÇÃO DE ESTIMATIVAS ROBUSTAS." Oecologia Australis 25, no. 02 (June 16, 2021): 344–66. http://dx.doi.org/10.4257/oeco.2021.2502.08.

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Age and growth are key elements on the fish population dynamics, allowing the age structure identification, age of first maturity, mortality rates and longevity calculations, among others. There are also age-based fisheries and ecological models that depends on these studies. It is a challenger field due to the intrinsic variability of growth process at individual and population levels, influenced by endogen and exogen factors. For freshwater fishes, the modal progression analysis, and the analysis of growth rings on scales are widespread applied for age and growth. Like all methods, premises and limitations exist and they must be observed. Otolith analysis is more accurate, unless difficult to stream fishes due to sample size requirements, small otolith size and rings record, specially at the Neotropical region. In this study, sample techniques, material and data acquirement and analyses are presented, focusing stream fishes. Limitations were identified in the results available in the scientific literature due to no consideration of methods premises allied to the mechanic use of software and manuals. For further studies, length frequency analysis, otolith daily rings and experiments are recommended to estimate age and growth of fishes from brooks and streams.
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Rosa, Alaina Cristine, and Fernanda de Oliveira Martins and Francisco Langeani. "Miniaturization in Otothyris Myers, 1927 (Loricariidae: Hypoptopomatinae)." Neotropical Ichthyology 12, no. 1 (March 2014): 53–60. http://dx.doi.org/10.1590/s1679-62252014000100005.

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Many species of freshwater fishes are considered miniatures, nevertheless, their identification as miniatures is frequently based only on an arbitrary criterion of small body size. Although some species of Otothyris had been suggested to be miniature, informations about the process and consequences of miniaturization in the genus are lacking. Here, we detail developmental events of loss, fusion, and modifications in several bones, the laterosensory system, and even the brain, that in sum demonstrate miniaturization in all species of the genus. Our results may be useful in the recognition of other miniature species of loricariids.
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50

Adamu, K. M., H. Muhammad, S. U. Ahmad, M. M. Ahmad, and A. M. Yakubu. "Diversity of Bacteria and Fungi Associated with Freshwater Fishes from Mijawal River, Nasarawa, Nigeria." Journal of Applied Sciences and Environmental Management 24, no. 6 (July 17, 2020): 1085–2020. http://dx.doi.org/10.4314/jasem.v24i6.22.

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Understudying the presence of bacteria and fungi population on freshwater fishes, provides the knowledge and understanding of their diversity and potential roles they may play in the health of the aquatic and terrestrial ecosystems. The present study was conducted to evaluate the diversity of bacteria and fungi species of identified fish samples from River Mijawal, Nasarawa State. The fishes were sampled from the only landing for three months (October to December, 2019) for this study. During the sampling periods, thirteen (13) different fish species were identified; they were swabbed with sterilized swab stick from the skin surface for bacteria and fungi identification. The Dominant fish species were Tilapia zillii, Labeo senegalensis, Mormyrus rume and Mormyrus tapines. The total number of thirteen (13) bacterial and five (5) fungi species were isolated and identified. Eight (8) of the bacteria isolates, were gram negative bacteria (Pseudomonas sp, Aeromonas sp, Serratia mercescenes, Proteus sp, Salmonella spp, Escherichia coli, Klebsiella sp, and Enterobacter sp.); whilst the other five (5) were gram positive bacteria (Bacillus sp, Micrococcus sp, Staphylococcus sp, Enterococcus sp and Listeria monocytogenes).The five isolated fungi species were Aspergillus niger, Rhizopus sp, Penicillium sp, Trichoderma viridae and Geotrichum candidum. The frequency of occurrence of the isolated bacteria indicated that Staphylococcus sp had the highest frequency of occurrence (16.67%) while Serratia mercescens had the least occurrence (2.78%). The highest number of occurrence of the isolated fungi species was observed in Rhizopus sp while the least was recorded in Geotricum candidum. It was observed that Tilapia zillii and Mormyrus rume had the most frequent bacteria and fungi isolates. It can therefore be deduced from the results that fish samples identified from River Mijawal has high bacteria and fungi diversity that may be pathogenic to man. The occurrence of these microbes may be attributed of human and animal faeces in the River Keywords: Freshwater fishes, Microbes, River Mijawal, Nasarawa
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