Journal articles on the topic 'Fresh lactic curd cheese'
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Samelis, John, Agapi I. Doulgeraki, Vasiliki Bikouli, Dimitrios Pappas, and Athanasia Kakouri. "Microbiological and Metagenomic Characterization of a Retail Delicatessen Galotyri-Like Fresh Acid-Curd Cheese Product." Fermentation 7, no. 2 (April 29, 2021): 67. http://dx.doi.org/10.3390/fermentation7020067.
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This study evaluated the microbial quality, safety, and ecology of a retail delicatessen Galotyri-like fresh acid-curd cheese traditionally produced by mixing fresh natural Greek yogurt with ‘Myzithrenio’, a naturally fermented and ripened whey cheese variety. Five retail cheese batches (mean pH 4.1) were analyzed for total and selective microbial counts, and 150 presumptive isolates of lactic acid bacteria (LAB) were characterized biochemically. Additionally, the most and the least diversified batches were subjected to a culture-independent 16S rRNA gene sequencing analysis. LAB prevailed in all cheeses followed by yeasts. Enterobacteria, pseudomonads, and staphylococci were present as <100 viable cells/g of cheese. The yogurt starters Streptococcus thermophilus and Lactobacillus delbrueckii were the most abundant LAB isolates, followed by nonstarter strains of Lactiplantibacillus, Lacticaseibacillus, Enterococcus faecium, E. faecalis, and Leuconostoc mesenteroides, whose isolation frequency was batch-dependent. Lactococcus lactis isolates were sporadic, except for one cheese batch. However, Lactococcus lactis, Enterobacteriaceae, Vibrionaceae, Salinivibrio, and Shewanellaceae were detected at fairly high relative abundances culture-independently, despite the fact that their viable counts in the cheeses were low or undetectable. Metagenomics confirmed the prevalence of S. thermophilus and Lb. delbrueckii. Overall, this delicatessen Galotyri-like cheese product was shown to be a rich pool of indigenous nonstarter LAB strains, which deserve further biotechnological investigation.
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RAMKUMAR, CHIKKANNA, LAWRENCE K. CREAMER, KEITH A. JOHNSTON, and RODNEY J. BENNETT. "Effect of pH and time on the quantity of readily available water within fresh cheese curd." Journal of Dairy Research 64, no. 1 (February 1997): 123–34. http://dx.doi.org/10.1017/s0022029996001914.
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Some of the textural changes that occur early in cheese maturation may be related to the redistribution of water within the cheese matrix. To examine this, a model cheese curd system was devised and explored. Initially, cheese curd was prepared using starter and chymosin and the curd pH was controlled by varying the draining and salting pH values. The quantity of serum that could be centrifuged from the resultant curd was less for lower pH curd and decreased in volume with time. The curd pH decreased with time. In the protocol finally adopted, milk was acidified with lactic acid and coagulated with Rennilase 46L. After cheddaring, salting and light pressing, the samples of this curd were finely diced and mixed with glucono-δ-lactone to give curd samples with comparable moisture contents, similar casein proteolysis rates but different pH values. The quantity of serum that could be centrifuged from these samples was greater for pH 5·6 curd than for pH 5·2 curd and decreased faster for the lower pH curd. Neither the curd moisture nor the pH changed significantly during curd storage and the casein proteolysis was low. These results for the model curd system are consistent with known water absorption characteristics of casein curd under ‘equilibrium’ conditions and the effects of pH and mineral salts on this absorption. It was concluded that, during the early stages of cheese ripening, there may be a redistribution of moisture within the cheese, related to the basic properties of the protein matrix and the transient effects of curd salting, rather than as a direct consequence of glycolytic and proteolytic changes.
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Sustova, Kvetoslava, Jiri Mlcek, Tana Luzova, and Jan Kuchtik. "Utilization of FT-NIR Spectroscopy to Check Acidity of Various Types of Cheeses." Journal of AOAC INTERNATIONAL 102, no. 3 (May 1, 2019): 893–97. http://dx.doi.org/10.5740/jaoacint.18-0250.
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Abstract Background: Monitoring the acidity of cheese is an important control mechanism in various stages of manufacture, including aging. Acid development in cheesemaking is essential to cheese flavor, texture, and safety. Objective: The aim of the work was to develop and validate calibration models by using NIR spectroscopy, which allows for the monitoring of changes in cheese acidity (pH and titration acidity) during cheese ripening. Methods: Cheeses were analyzed by an FT-NIR spectrometer. Each of the samples was analyzed three times, and for calibration, an average spectrum was used. A partial least-squares regression was used to develop calibration models. The constructed calibration models were validating by full cross-validation. Results: Calibration models were created with a high correlation coefficient for the following cheese pH levels: blue cheese (0.966), Olomouc curd read smear cheese (0.984), and fresh goat cheese (0.980). Results of the calibration of titratable acidity are functional for fresh goat cheese (0.953) and mozzarella (0.999). Conclusions: The results of these new calibration methods showed the possibility of NIR technology for the fast determination of pH and titratable acidity. Highlights: Detection of cheese acidity using FT-NIR spectrometry enables rapid evaluation of the process of lactic acidification in particular cheese technological operations, including the maturing of cheeses.
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Barbaccia, Pietro, Gabriele Busetta, Michele Matraxia, Anna Maria Sutera, Valentina Craparo, Giancarlo Moschetti, Nicola Francesca, Luca Settanni, and Raimondo Gaglio. "Monitoring Commercial Starter Culture Development in Presence of Red Grape Pomace Powder to Produce Polyphenol-Enriched Fresh Ovine Cheeses at Industrial Scale Level." Fermentation 7, no. 1 (March 9, 2021): 35. http://dx.doi.org/10.3390/fermentation7010035.
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Red grape Nero d’Avola cultivar grape pomace powder (GPP) was applied during fresh ovine cheese production in order to increase polyphenol content. Before cheeses were produced, the bacteria of a freeze-dried commercial starter culture were isolated and tested in vitro against GPP. Two dominant strains, both resistant to GPP, were identified. Thestarter culture was inoculated in pasteurized ewe’s milk and the curd was divided into two bulks, one added with 1% (w/w) GPP and another one GPP-free. GPP did not influence the starter culture development, since lactic acid bacteria (LAB) counts were 109 CFU/g in both cheeses at 30 d. To exclude the interference of indigenous LAB, the pasteurized milk was analyzed, and several colonies of presumptive LAB were isolated, purified and typed. Four strains were allotted into Enterococcus and Lacticaseibacillus genera. The direct comparison of the polymorphic profiles of cheese bacteria evidenced the dominance of the starter culture over milk LAB. The addition of GPP increased cheese total phenolic compounds by 0.42 g GAE/kg. Sensory evaluation indicated that GPP-enriched cheese was well appreciated by the judges, providing evidence that GPP is a suitable substrate to increase the availability of total phenolic content in fresh ovine cheese.
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Warncke, Malou, Sonja Keienburg, and Ulrich Kulozik. "Cold-Renneted Milk Powders for Cheese Production: Impact of Casein/Whey Protein Ratio and Heat on the Gelling Behavior of Reconstituted Rennet Gels and on the Survival Rate of Integrated Lactic Acid Bacteria." Foods 10, no. 7 (July 11, 2021): 1606. http://dx.doi.org/10.3390/foods10071606.
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The idea was to develop powders for fresh/hard cheese or quark production comprising milk proteins in optimal composition and functional properties for manufacturing each of those cheese types. The aim was to avoid whey protein drainage by their prior removal or by their heat-induced structural integration in the curd. The pre-renneted powders already contain additives such as starter cultures and calcium chloride to instantaneously form homogeneous curds upon reconstitution. The impact of the casein/whey protein ratio (86:14 by ultrafiltration and 98:2 by microfiltration) and upfront heat treatment (80 °C/30 min) on the gelling behavior of reconstituted rennet gels and on the survival rate of integrated Lactobacillus paracasei ssp. paracasei F19 was investigated. The assessment criteria for the rennet gelation were curd firming rate, gel strength, and whey drainage. Furthermore, the amount of integrated whey proteins and the resulting cheese yield were evaluated. It could be shown that heating had a positive effect on the viable cell count of the bacteria after spray drying and on the gelation behavior of the reconstituted ultrafiltration concentrates. The curd firming rate and the gel strength could be increased to higher values than the reconstituted microfiltration concentrate at 25% total solids.
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Bulut, Cisem, Hatice Gunes, Burcu Okuklu, Sebnem Harsa, Sevda Kilic, Hatice Sevgi Coban, and Ali Fazil Yenidunya. "Homofermentative lactic acid bacteria of a traditional cheese, Comlek peyniri from Cappadocia region." Journal of Dairy Research 72, no. 1 (January 14, 2005): 19–24. http://dx.doi.org/10.1017/s0022029904000536.
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Comlek peyniri is a typical artisanal cheese in Central Anatolia. This type of cheese was made by using the indigenous lactic acid bacteria (LAB) flora of cow or ewes' milk. Majority of the samples were taken from fresh cheese because the aim was to isolate homofermentative LAB. Initially 661 microbial isolates were obtained from 17 cheese samples. Only 107 were found to be homofermentative LAB. These isolates were selected and identified by using both phenotypic and molecular methods. Phenotypic identification included curd formation from skim milk, catalase test, Gram staining and light microscopy, growth at different temperatures and salt concentrations, arginine hydrolysis, gas production from glucose, and carbohydrate fermentation. Molecular identification was based on the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 16S rRNA gene-ITS (internally transcribed spacer) region. By combining the phenotypic and molecular identification results, isolates belonging to each of the following genera were determined at species or subspecies level: 54 Lactococcus lactis subsp. lactis, 21 Enterococcus faecium, 3 Ec. faecalis, 2 Ec. durans, 10 Ec. sp., 15 Lactobacillus paracasei subsp. paracasei, and 2 Lb. casei strains. Technological characterisation was also performed by culturing each of the strains in UHT skim milk, and by monitoring pH change and lactic acid production at certain time intervals through the 24 h incubation. Results of the technological characterisation indicated that 33% of the isolates (35 strains) were capable of lowering the pH of UHT milk below 5·3 after 6 h incubation at 30 °C. Thirty four of these strains were Lc. lactis subsp. lactis, and only one was an Ec. faecium strain.
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Albuja Landi, Ana Karina, Janneth Gallegos, Paola Vargas Cali, and Paola Arguello Hernández. "THERAPEUTIC ADHERENCE IN PATIENTS WITH CHRONIC DISEASES OF THE CLUB OF OLDER ADULTS OF A HEALTH CENTER, ECUADOR." Anales de la Real Academia Nacional de Farmacia, no. 86(02) (2020): 117–24. http://dx.doi.org/10.53519/analesranf.2020.86.02.03.
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One of the traditional fresh cheeses in Ecuador is the artisanal leafcheese, a kind of stretched-curd cheese. The artisanal product is wrapped in achira leaves (Canna indica), while the industrial leafcheese is packed at vacuum in high density polyethylene bags. In this study the microbiological quality of both products was compared. The hygienic-sanitary microbial indicators and lactic acid bacteria (LAB) were quantified. The LAB isolated were characterized phenotypically. The samples were obtained from artisanal cheese-making and industrial located in Latacunga city province Cotopaxi. The total aerobic mesophilic count was made based on national regulations (NTE INEN 1529 5); total coliforms, Escherichia coli and Staphylococcus aureus was evaluated using petrifilm methods (AOAC 991.14 – AOAC 2003.07) and to LAB was used PRT-712.02-047. The results show high quantities of total coliforms, E. coli and S. aureus in both products, these data exceed the limits of acceptability established in Ecuadorian regulations, this evidence poor hygienic quality of the processes or incorrect controls of milk as raw material. The lactic acid bacteria count showed statistically significant differences, the industrial cheeses had a reduction of 18,15% of Lactobacillus and 14,27% of Lactococcus compared with artisanal cheeses. A total of 32 strains of lactic acid bacteria were isolated, these showed similar phenotypic characteristics, but these had a different response at the level of pH (4,4;9,4) and NaCl (6,5%). The sensory evaluation will be an important complement in this type of study.
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Ruvalcaba-Gómez, José M., Raúl J. Delgado-Macuil, Lily X. Zelaya-Molina, Otoniel Maya-Lucas, Edmundo Ruesga-Gutiérrez, Luis M. Anaya-Esparza, Zuamí Villagrán-de la Mora, David A. López-de la Mora, and Ramón I. Arteaga-Garibay. "Bacterial Succession through the Artisanal Process and Seasonal Effects Defining Bacterial Communities of Raw-Milk Adobera Cheese Revealed by High Throughput DNA Sequencing." Microorganisms 9, no. 1 (December 23, 2020): 24. http://dx.doi.org/10.3390/microorganisms9010024.
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The bacterial community of the artisanal Adobera cheese from Los Altos de Jalisco was described through high-throughput sequencing of 16S rRNA gene libraries. Samples were collected in two different seasons (dry and rainy) during four key steps of the manufacturing process (raw milk, fresh curd, matured curd, and cheese). Bacterial diversity was higher in early steps in comparison with the final elaboration stages. Firmicutes and Proteobacteria were the most abundant phyla, strongly represented by the Streptococcaceae, Enterobacteriaceae and Lactobacillaceae families, and core bacteria genera such as Streptococcus spp., Lactococcus spp., and Lactobacillus spp. Undesirable bacteria, including Pseudomonas spp. and Acinetobacter spp., were also detected in raw milk but almost undetectable at the end of the cheese manufacturing process, and seemed to be displaced by lactic-acid bacteria-related genera. Seasonal effects were observed on the community structure but did not define the core microbiota composition. Predictive metabolism was related to membrane transport, and amino-acid, lipid, and carbohydrate metabolism pathways. Our results contribute to deduce the role of bacteria involved in Adobera cheese manufacturing in terms of the metabolism involved, cheese microbial safety, and how undesirable bacterial populations could be regulated by process standardization as a potential tool to improve safety.
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SAAD, SUSANA M. I., CÉZAR VANZIN, MARICÊ N. OLIVEIRA, and BERNADETTE D. G. M. FRANCO. "Influence of Lactic Acid Bacteria on Survival of Escherichia coli O157:H7 in Inoculated Minas Cheese during Storage at 8.5°C." Journal of Food Protection 64, no. 8 (August 1, 2001): 1151–55. http://dx.doi.org/10.4315/0362-028x-64.8.1151.
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Minas cheese is a typical Brazilian fresh cheese, manufactured by addition of rennin and CaCl2 to milk, followed by draining the curd. The intrinsic characteristics of this product make it favorable for growth of pathogens, including Escherichia coli O157:H7. The influence of the addition of a commercial mesophilic type O lactic culture to this product on the growth of this pathogen during storage at 8.5°C was evaluated. Eight different formulations of Minas cheese were manufactured using raw or pasteurized milk and with or without salt and lactic culture. Individual portions of each formulation were transferred to sterile plastic bags and inoculated with E. coli O157:H7 to yield ca. 103 or 106 CFU/g. After blending by hand massaging the bags, samples were stored at 8.5°C for up to 14 days. E. coli O157:H7 was counted after 1, 2, 7, and 14 days of storage using 3M Petrifilm Test Kit-HEC. Counts in samples without added lactic culture showed a 2-log increase in the first 24 h and remained constant during the following 14 days. Counts in samples with added lactic culture showed a 0.5-log increase in the first 24 h, followed by a decrease. These variations were statistically significant (P < 0.05). No significant variations (P > 0.05) were obtained for cheese samples manufactured with pasteurized or raw milk, with or without salt. Results indicate that the addition of type O lactic culture may be an additional safeguard to well-established good manufacturing practices and hazard analysis and critical control point programs in the control of growth of E. coli O157:H7 in Minas cheese.
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Iannella, Giuseppe. "Camel, Donkey and Horse Cheese Making with a High Transformation Yield by Natural Thickeners and Lactic Coagulation." Food Science and Nutrition Studies 4, no. 3 (September 8, 2020): p1. http://dx.doi.org/10.22158/fsns.v4n3p1.
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Making cheese from donkey and mares milk is considered unfeasible, due to difficulties in coagulation and curd forming. Encyclopedia of Dairy Sciences 2nd edition (2011) reported that no cheese is made from equid milk. However in 2015 a pioneering study of Iannella have reported the first protocols for making fresh donkey and mares cheese, subsequently there have been only a few protocols by other researchers. Anyhow, the low cheese yield of these protocols together with a higher cost of production of the raw material currently limit practical application. Also the processing of camel milk into cheese is technically more difficult than milk from other domestic dairy animals which may relate to the poor rennetability of camel milk. Therefore, a research project was planned by Iannella which has developed a technological procedure to produce cheese from camel, donkey and mares milk with a high transformation yield by the use of locust bean gum, k- carrageenan and lactic coagulation in a dedicated process, thus with a mini mal adjustments in the manufacturing technology and equipment. The whey proteins of milk with this method are withheld and this improve the efficiency of making cheese and increase further yield of cheese however preserving body and texture similar of cheese prepared by conventional processes. In the near future this protocol or similar, they could represent a source of innovative cheese and the development definitive of a new commercial scale of cheese from donkey, mares or camel milk or from others minor milks at the same time improving food and animal biodiversity and therefore all the ensuing benefits.
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Daryaei, H., M. J. Coventry, C. Versteeg, and F. Sherkat. "Effect of high pressure treatment on starter bacteria and spoilage yeasts in fresh lactic curd cheese of bovine milk." Innovative Food Science & Emerging Technologies 9, no. 2 (April 2008): 201–5. http://dx.doi.org/10.1016/j.ifset.2007.06.011.
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SAMELIS, JOHN, ALEXANDRA LIANOU, ATHANASIA KAKOURI, CÉLINE DELBÈS, IRENA ROGELJ, BOJANA BOGOVIČ-MATIJAŠIĆ, and MARIE-CHRISTINE MONTEL. "Changes in the Microbial Composition of Raw Milk Induced by Thermization Treatments Applied Prior to Traditional Greek Hard Cheese Processing." Journal of Food Protection 72, no. 4 (April 1, 2009): 783–90. http://dx.doi.org/10.4315/0362-028x-72.4.783.
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The microbiological quality, safety, and composition of mixtures of ewe's and goat's milk (90:10) used for cheesemaking were evaluated before and after thermization at 60 and 67°C for 30 s. Such mild thermal treatments are commonly applied to reduce natural contaminants of raw milk before processing for traditional hard Greek cheeses. Raw milk samples had an average total bacterial count of 7.3 log CFU/ml; most of these bacteria were lactic acid bacteria (LAB) and pseudomonads. The LAB flora of raw milk was dominated by enterococci (40.8%), followed by lactococci (20.4%), leuconostocs (18.4%), and mesophilic lactobacilli (10.2%). Enterococcus faecalis (30.1%) and Enterococcus faecium (13.7%) were the most common LAB isolates, followed by Enterococcus durans, Lactococcus lactis subsp. lactis, Lactobacillus plantarum, and Leuconostoc lactis. Thermization at 60°C for 30 s was effective for reducing raw milk contamination by enterobacteria (5.1 log CFU/ml), coagulase-positive staphylococci (3.3 log CFU/ml), and Listeria (present in 25-ml samples) to safe levels, but it also reduced mesophilic lactococci, leuconostocs, lactobacilli, and selected enterococci (72.0%) in thermized milk. Thermization at 67°C for 30 s had a major inactivation effect on all bacterial groups. Two nisin-producing L. lactis subsp. lactis strains (M78 and M104) were isolated from raw milk, but neither nisin-producing nor other bacteriocin-producing LAB strains were isolated from thermized milk. Thus, thermization treatments control harmful bacteria but also may have a negative impact on milk quality by reducing desirable LAB and the biodiversity of raw milk bacteria overall, inactivating potentially protective LAB strains and enhancing the ability of potentially pathogenic enterococci to grow in fresh cheese curds.
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Matijašić, Bojana Bogovič, Jernej Oberčkal, Petra Mohar Lorbeg, Diana Paveljšek, Nina Skale, Borut Kolenc, Špela Gruden, Nataša Poklar Ulrih, Marko Kete, and Maja Zupančič Justin. "Characterisation of Lactoferrin Isolated from Acid Whey Using Pilot-Scale Monolithic Ion-Exchange Chromatography." Processes 8, no. 7 (July 9, 2020): 804. http://dx.doi.org/10.3390/pr8070804.
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The aim of this study was to characterize the properties of lactoferrin (LF) obtained in a process developed for its isolation from acid whey derived from the production of fresh curd cheese, using a unique technology of ion-exchange chromatography on CIM® monolithic columns. The freeze-dried lactoferrin samples produced on the pilot plant (capacity 1 m3) were examined for the purity, iron-binding capacity, antibacterial activity, and pH- and temperature-stability. Apo-LF inhibited several tested strains (enterobacteria, Staphylococcus, Streptococcus salivarius) except clostridia, lactic acid bacteria, and bifidobacteria. Sample of LF intentionally saturated with Fe3+ lost its antibacterial activity, indicating the involvement of mechanisms based on depriving bacteria of an iron source. All samples, regardless of the iron-saturation level, exhibited stability in pH range 4.0 to 11.0. LF with higher iron content (A-value = 41.9%) showed better thermal stability. Heat treatment up to 72 °C/3 s did not reduce antimicrobial activity against E. coli O157: H7 tox-. Higher purity (above 91%), higher iron-binding capacity and higher inhibitory activity against E. coli O157: H7 tox- compared to some similar products from the market was observed. These results demonstrate a high potential of monolithic ion-exchange chromatography for industrial processing of acid whey as a source of LF that can be used in new products with high-added value. The upscaling of the process is ongoing on a demonstration plant (10–30 m3/day capacity).
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Manzo, Nadia, Loredana Biondi, Donatella Nava, Federico Capuano, Fabiana Pizzolongo, Alberto Fiore, and Raffaele Romano. "On The Possibility to Trace Frozen Curd in Buffalo Mozzarella Cheese." Journal of Food Research 6, no. 1 (January 13, 2017): 104. http://dx.doi.org/10.5539/jfr.v6n1p104.
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The manufacturing of Buffalo Mozzarella PDO (Protected Designation of Origin) cheese requires the exclusive use of fresh buffalo milk, which must be transformed into cheese within 60 hours after milking. The limited availability of buffalo milk and simultaneous increase in Mozzarella demand during the summer cause producers to use frozen intermediates (milk and/or curd) in the cheese-making process. These practices are not allowed. Few data are available in the literature about the effects of freezing on buffalo milk and curd. Recent studies demonstrated that the use of frozen buffalo milk can be detected in mozzarella cheese based on the increase in casein fragment γ4-CN. This work aims to verify the possibility of tracing the presence of frozen curd in Buffalo Mozzarella PDO cheese. The electrophoresis technique was used to reveal the presence of γ4-CN. Equivalent concentrations of this fragment were found in fresh and frozen curd that were stored for 9 months. Our results suggest that γ4-CN cannot be used to discriminate fresh PDO Mozzarella and Mozzarella cheese produced from frozen curd. A second objective of the work was to evaluate the effects of freezing on curd lipids. In particular, the fatty acid and mono-diglyceride profiles were evaluated. Significant differences were found in the amounts of 1,2-Dipalmitin and 1,3-Diolein between fresh curd and curd that was stored for 9 months at freezing temperatures. Although some significant differences were found in the mono-diglyceride profiles, no objective marker that can distinguish between fresh and frozen products is currently available.
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ABDALLA, O. M., P. M. DAVIDSON, and G. L. CHRISTEN. "Survival of Selected Pathogenic Bacteria in White Pickled Cheese Made With Lactic Acid Bacteria or Antimicrobials." Journal of Food Protection 56, no. 11 (November 1, 1993): 972–76. http://dx.doi.org/10.4315/0362-028x-56.11.972.
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Effect of lactic acid bacteria starter culture, nisin, hydrogen peroxide, or potassium sorbate on Listeria monocytogenes, Staphylococcus aureus, and Salmonella typhimurium in white pickled cheese made from pasteurized milk with 4% salt and preserved in 4% brine solution at 4°C for 60 d was studied. The starter culture inhibited all three pathogens while antimicrobials did not. Beyond day 50 in curd and day 30 in brine solution, L. monocytogenes was not detected by direct plating in cheese with added starter culture. S. aureus was not detected after day 30 in curd and day 20 in brine solution in the same cheese. S. typhimurium was not detected after day 30 in cheese curd and was not detected in brine solution at any time with lactic acid bacteria starter culture added. The pH of brine solution of starter treatment dropped below 4.7 in all experiments, while antimicrobial treatments all had a pH >5.5.
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Tipvarakarnkoon, Tatsawan, Sangrawee Sornsa-ard, and Wutcharee Imcha. "Development of fresh cheese made from coconut milk in combination with cow’s milk." British Food Journal 119, no. 10 (October 2, 2017): 2183–93. http://dx.doi.org/10.1108/bfj-10-2016-0503.
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Purpose The purpose of this paper is to develop fresh cheese made from different types of coconut milk (fresh, UHT, and reconstituted coconut milk) mixed with pasteurized cow’s milk (50:50) by rennet coagulation. The main aim of this study was to achieve successful formation of cheese curd with fully coconut milk aroma and flavor. Design/methodology/approach Fresh cheese made from different types of coconut milk mixed with cow’s milk (50:50) were developed and compared to fresh cheese made from cow’s milk (100:0). Physicochemical analysis including pH, acidity, percentage of yield, protein and fat content, texture analysis and sensory evaluation has been performed. The nine-point hedonic preference test (n=35) and consumer testing (n=235) were conducted. Findings In comparison, fresh cheese made with the presence of coconut milk (50:50) had higher fat content, lower crude protein content, higher firmness, and adhesiveness than those made from cow’s milk alone (0:100). The cheese with the presence of coconut milk was liked better by consumers (7.35 out of 9). Rehydrated coconut milk was mostly preferred to use among all three types of coconut milk which showed firmer texture and admired flavor. The texture of the developed product was proved to be similar to commercial cottage cheese. Using the rehydrated coconut milk in the formulation gave successful results for making fresh coconut milk cheese, which has also not been used previously in any research. Research limitations/implications In this paper, the effect of process conditions on the quality of cheese curd formation was not yet investigated and discussed. The process parameters including heat treatment and renneting time should be further studied. Originality/value A novel fresh cheese made from coconut milk mixed with pasteurized cow’s milk was successfully developed. The cheese curd was formed with fully coconut milk aroma and flavor.
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Faccia, Michele, Giuseppe Gambacorta, Giovanni Martemucci, Graziana Difonzo, and Angela Gabriella D’Alessandro. "Chemical-Sensory Traits of Fresh Cheese Made by Enzymatic Coagulation of Donkey Milk." Foods 9, no. 1 (December 23, 2019): 16. http://dx.doi.org/10.3390/foods9010016.
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Making cheese from donkey milk is considered unfeasible, due to difficulties in coagulation and curd forming. Two recent studies have reported the protocols for making fresh cheese by using camel chymosin or calf rennet, but the chemical and sensory characteristics of the products were not thoroughly investigated. The present paper aims to give a further contribution to the field, by investigating cheesemaking with microbial rennet and evaluating the chemical composition, total fatty acid, volatile organic compounds (VOCs) and sensory profile of the resultant product. Six trials were undertaken at laboratory scale on donkey milk from a Martina Franca ass, by applying the technological scheme as reported for calf rennet, with some modifications. Bulk cow milk was used as a control. Donkey milk coagulated rapidly, but the curd remained soft, and was only suitable for making fresh cheese; differently, cow milk coagulated almost instantaneously under these strong technological conditions, giving rise to a semi-hard curd in very short time. The moisture level of donkey cheese was almost the same as reported in the literature, whereas the yield was higher, probably due to the high protein content of the milk used. The total fatty acid composition of cheese presented some differences with respect to milk, mostly consisting in a higher presence of saturated compounds. A connection with a better retention of the large sized fat globules into the curd was hypothesised and discussed. The VOC analyses, performed by solid-phase micro extraction gas chromatography-mass spectrometry, allowed the identification of 11 compounds in milk and 18 in cheese. The sensory characteristics of donkey cheese were strongly different with respect to the control, and revealed unique and pleasant flavours.
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XU, YI, and JIAN KONG. "Construction and Potential Application of Controlled Autolytic Systems for Lactobacillus casei in Cheese Manufacture." Journal of Food Protection 76, no. 7 (July 1, 2013): 1187–93. http://dx.doi.org/10.4315/0362-028x.jfp-12-307.
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The rapid release of intracellular enzymes into the curd by the autolysis of lactic acid bacteria starters is universally recognized as a critical biological process to accelerate cheese ripening. Lactobacillus casei is typically the dominant nonstarter lactic acid bacterium in the ripening cheese. In this study, two controlled autolytic systems were established in L. casei BL23, based on the exploitation of the autolysins sourced from Lactococcus lactis (AcmA) and Enterococcus faecalis (AtlA). The lysis abilities of the systems were demonstrated both in broth and a model cheese, in which a fivefold increase in lactate dehydrogenase activity was detected in the curd with sufficient viable starter cells being maintained, indicating that they could lead to the timely release of intracellular enzymes.
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Alonso, Rocío, Antonia Picon, Pilar Gaya, and Manuel Nuñez. "Proteolysis, lipolysis, volatile compounds and sensory characteristics of Hispánico cheeses made using frozen curd from raw and pasteurized ewe milk." Journal of Dairy Research 80, no. 1 (December 20, 2012): 51–57. http://dx.doi.org/10.1017/s0022029912000738.
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Hispánico cheese, manufactured from a mixture of cow and ewe milk, is representative of cheese varieties made using milk from more than one animal species in Mediterranean countries. The shortage of ewe milk production in autumn hinders the uniformity of Hispánico cheese composition throughout the year. To surmount this inconvenience of ewe milk seasonality, curds made in spring from raw and pasteurized ewe milk were stored frozen and used four months later for the manufacture of Hispánico cheese. Experimental cheeses were made by mixing fresh curd from pasteurized cow milk with thawed curd from raw or pasteurized ewe milk, and control cheese from a mixture of pasteurized cow and ewe milk in the same proportion. Characteristics of experimental and control cheeses throughout a 60-d ripening period were investigated. On the one hand, the experimental cheese containing frozen curd from raw ewe milk showed the highest counts of staphylococci, Gram-negative bacteria and coliforms, the highest levels of aminopeptidase and esterase activity, and the highest concentrations of free amino acids, free fatty acids, alcohols and esters. On the other, the experimental cheese containing frozen curd from pasteurized ewe milk had concentrations of free amino acids, free fatty acids and volatile compounds similar to those of control cheese, with the only exception being a higher level of ketones. Flavour intensity reached the highest scores in the experimental cheese containing frozen curd from raw ewe milk, followed by the experimental cheese containing frozen curd from pasteurized ewe milk. Flavour quality scores of both experimental cheeses were similar, and lower than those of control cheese.
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Graça, Carla, Anabela Raymundo, and Isabel Sousa. "Improving the Technological and Nutritive Properties of Gluten-Free Bread by Fresh Curd Cheese Enrichment." Applied Sciences 10, no. 19 (September 30, 2020): 6868. http://dx.doi.org/10.3390/app10196868.
Full textAbstract:
Replacing wheat flour in the breadmaking process is a technology challenge since the elimination of gluten has a strong influence on bread quality. Proteins addition are often used to form a protein network capable of mimicking gluten-like structure, giving to dough a foaming support. This study aimed to evaluate the potential of denatured whey proteins coming from fresh curd cheese addition, to strengthening gluten-free dough structure, enhancing the breadmaking performance. Curd cheese additions were tested (5% up to 20%, weight/weight) and the effect on dough rheology behavior and bread quality was evaluated. Findings obtained revealed that the technology and nutritional properties of the bread can be enhanced by curd cheese addition, and such effects should be related to the composition and functionality of denatured whey proteins. Considering higher levels of curd cheese (20%) tested, improvements on bread quality was observed, leading to a considerable increase in bread volume (73%), softness (65%), with a significant reduction on staling kinetics (70%), comparing with control bread. Additionally, an improvement in nutritional value in terms of proteins (80%) and minerals content (P—50.0%, Mg—6.0%, and Ca—360.3%) was obtained, which can give an additional contribution to the nutritional daily requirements of celiac patients. Linear correlations between dough rheology properties and bread quality attributes were found, supporting the good breadmaking performance obtained.
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Zamora, Anna, Victoria Ferragut, Joan Miquel Quevedo, Buenaventura Guamis, and Antonio-José Trujillo. "Ultra-high pressure homogenisation of milk: technological aspects of cheese-making and microbial shelf life of a starter-free fresh cheese." Journal of Dairy Research 79, no. 2 (February 17, 2012): 168–75. http://dx.doi.org/10.1017/s0022029912000052.
Full textAbstract:
Fresh cheeses from pasteurised (80°C for 15 s), homogenised–pasteurised (15+3 MPa at 60°C; 80°C for 15 s) or ultra-high pressure homogenised milks (300 MPa and inlet temperature of 30°C) were produced in order to evaluate different technological aspects during cheese-making and to study their microbial shelf life. Although the coagulation properties of milk were enhanced by ultra-high pressure homogenisation (UHPH), the cheese-making properties were somewhat altered; both conventional homogenisation and UHPH of milk provoked some difficulties at cutting the curd due to crumbling and improper curd matting due to poor cohesion of the grains. Cheese-milk obtained by UHPH showed a higher microbiological quality than milk obtained by conventional treatments. Starter-free fresh cheeses made from UHPH-treated milk showed less syneresis during storage and longer microbiological shelf-life than those from conventionally treated milk samples.
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Rinaldi, Simona, Giuliano Palocci, Sabrina Di Giovanni, Miriam Iacurto, and Carmela Tripaldi. "Chemical Characteristics and Oxidative Stability of Buffalo Mozzarella Cheese Produced with Fresh and Frozen Curd." Molecules 26, no. 5 (March 5, 2021): 1405. http://dx.doi.org/10.3390/molecules26051405.
Full textAbstract:
Milk and dairy products can have variable contents of antioxidant compounds that contribute to counteract the oxidation of lipids and proteins during processing and storage. The content of active antioxidant compounds is closely linked to their protection by oxidation. Freezing is one of the factors that can reduce antioxidant activity. Freezing of milk or curd is frequently used in case of the seasonality of milk production and/or seasonal increased demand for some products. In this paper, the effect of using frozen curd on the oxidative stability of buffalo Mozzarella cheese was evaluated. Samples of buffalo Mozzarella with different frozen curd content (0%, 5%, 20%, and 50%) were produced and analyzed at one and nine days. Mozzarella cheese with higher frozen curd content had a significant increase in redox potential parallel to the decrease in antioxidant activity, showing less protection from oxidation. Lipid and protein oxidation, expressed respectively by malondialdehyde and carbonyl content, increased significantly with increasing frozen curd. At nine days, carbonyls significantly increased while malondialdehyde content did not vary, showing that during storage, fat was more protected from oxidation than protein. The average carbonyl levels were comparable to those of some cooked cheeses, and the malondialdehyde levels were even lower. The results of this study stimulate the investigation of new strategies to decrease the oxidative damage in cheeses produced in the presence of factors decreasing oxidative stability.
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Macej, Ognjen, Snezana Jovanovic, Sanja Seratlic, and Miroljub Barac. "Fresh cheese production on the basis of milk-protein coaggregates." Biotehnologija u stocarstvu 20, no. 1-2 (2004): 119–29. http://dx.doi.org/10.2298/bah0402119m.
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Two variants of fresh cheese were prepared: cheese prepared from milk heated at 650C for 30 minutes (control samples) and those prepared from milk heated for 10 minutes at 850C (experimental samples). Second method caused the protein co aggregates formation. Results of these investigations suggested that the whey obtained during fresh cheese production from co aggregates had considerable lower total nitrogen content (0.0601%) and milk fat content (0.08%). These values represented 46.37% and 33.33% of the control samples respectively. Also, dry matter of experimental whey samples was lower for 3.72% than in the whey of control samples. The cheese prepared on the basis of co aggregates had for 5.11% lower total solids content than control samples. The average values of these and control samples were 28.38% and 33.49% respectively. This may be due to protein co aggregates properties and the different structure of cheese curd. Disregarding to the higher utilization of the milk fat, the lower milk fat content in total solids content of experimental cheese is the result of higher participation of total nitrogen.
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Guizani, N., M. AI-Mugheiry, and M. Al-Ruzeiki. "Microbiological, Physico-Chemical, and Biochemical Changes During the Ripening of a Camembert Cheese Made from Pasteurized Cow’s Milk." Journal of Agricultural and Marine Sciences [JAMS] 5, no. 1 (January 1, 2000): 1. http://dx.doi.org/10.24200/jams.vol5iss1pp1-7.
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Camembert cheese was manufactured from pasteurized cow’s milk by the traditional method in order to determine the changes in the microflora, physico-chemical, and biochemical characteristics over 30-day ripening period. The total bacteria counts were high in cheese throughout ripening with lactic acid bacteria being the main microbial group both on the surface as well as in the center of the curd. However, the microbial activity is more important on the surface than in the center. Each group of microorganisms showed a typical variation during ripening on the surface and in the center. External heterogenous microflora, with high population of yeast, molds (mainly P. camemberti), and halophilic bacteria, induced a total rate of proteolysis and lipolysis about 1.5 times greater on the surface than in the curd at the end of ripening. Migration of salt from the curd reached equilibration after 23 days of ripening. A faster decrease in the pH of the surface was observed and a gradient of pH between the surface and the center was maintained during the ripening period.
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Hallén, Elin, Anne Lundén, Toomas Allmere, and Anders Andrén. "Casein retention in curd and loss of casein into whey at chymosin-induced coagulation of milk." Journal of Dairy Research 77, no. 1 (November 26, 2009): 71–76. http://dx.doi.org/10.1017/s0022029909990434.
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Impact of milk protein composition on casein (CN) retention in curd during the milk coagulation process was studied using a model cheese making system. Individual milk samples from 110 cows in mid lactation of the Swedish Red and Swedish Holstein breeds with known genotypes of β-casein, κ-casein and β-lactoglobulin were defatted, coagulated with chymosin, subjected to syneresis and subsequent pressing simulated by centrifugation. The results indicated that κ-casein concentration of milk plays an important role in the curd formation process and initial syneresis (whey after cutting), whereas an increased CN ratio was associated with less casein in whey after simulated pressing. Increased κ-casein concentration of milk also characterized the milk samples with no measurable loss of casein in whey, compared with milk samples with casein lost in whey, both after cutting and after simulated pressing. Concentrations of αs1-casein, β-casein, and total casein in milk were positively associated with fresh curd yield, which showed a strong correlation with amount of casein retained in curd. No effect of protein genotype on fresh curd yield or casein in whey was found. The β-lactoglobulin BB genotype was associated with increased casein retention in curd, most likely due to the association of this genotype with CN ratio.
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PAPAGEORGIOU, DEMETRIOS K., and ELMER H. MARTH. "Fate of Listeria monocytogenes during the Manufacture, Ripening and Storage of Feta Cheese." Journal of Food Protection 52, no. 2 (February 1, 1989): 82–87. http://dx.doi.org/10.4315/0362-028x-52.2.82.
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The ability of Listeria monocytogenes to grow during the Feta cheese-making process, and to survive during ripening, and storage of the cheese was examined. Pasteurized whole cow's milk was inoculated to contain ca. 5.0 × 103 L. monocytogenes [strain Scott A or California (CA)] cfu/ml and made into Feta cheese according to standard procedure. Lactobacillus bulgaricus, and Streptococcus thermophilus (1:1, v/v) were used as starter culture (1%, v/v). Fresh cheese was placed into sterile 12% salt brine and was held at 22°C for 24 h. Then it was placed into sterile 6% salt brine and held 4 d at 22°C after which it was stored in the same brine at 4°C. Milk, curd, whey, cheese, and brine were tested for numbers of L. monocytogenes and pH. Duplicate samples were used to enumerate L. monocytogenes by surface-plating on McBride Listeria Agar. Selected Listeria colonies were confirmed biochemically, L. monocytogenes was entrapped in curd during cheese-making with the population in curd being 0.92 Log10 cfu/g greater than in the inoculated milk; the whey contained an average of 3.2% of the initial inoculum. L. monocytogenes in cheese increased in numbers by ca. 1.5 Log10 cfu/g during the first 2 d of ripening, the population was 2.33 (S.D. ± 0.12) Log10 cfu/g greater in cheese than in the inoculated milk, with a maximum number of 1.5 × 106 cfu/g. The pH value of 2-d-old cheese decreased to 4.6 and then growth of L. monocytogenes ceased. Both strains of L. monocytogenes survived in Feta cheese for more than 90 d even at the low pH of 4.30 (S.D. ± 0.05) that Feta cheese had after ripening. Strain CA was significantly (P<0.006) less tolerant than strain Scott A, of conditions in the cheese during storage at 4°C.
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COPPOLA, RAFFAELE, MAURO NANNI, MASSIMO IORIZZO, ALIDA SORRENTINO, ELENA SORRENTINO, and LUIGI GRAZIA. "Survey of lactic acid bacteria isolated during the advanced stages of the ripening of Parmigiano Reggiano cheese." Journal of Dairy Research 64, no. 2 (May 1997): 305–10. http://dx.doi.org/10.1017/s0022029996002038.
Full textAbstract:
Parmigiano Reggiano is a hard, cooked cheese produced in specific areas of Northern Italy. The raw material is obtained by mixing the partly skimmed evening milk and whole morning milk. The mixture is then heated to 22°C, and natural whey starter is added at 28–30 g/l after 1–2 min, bringing the pH of the mixture to 6·2–6·3. After coagulation, which takes ∼15 min and occurs at 32–33°C owing to the addition of calf rennet powder, the curd is broken up for 2–4 min, cut into fragments and cooked at a temperature raised gradually to 42–44°C and then more quickly to 55–56°C over 10–15 min. The curd is left undisturbed, covered by the whey, for 40–60 min, then removed and placed inside a circular wooden mould. The cheese is held at ∼20°C for 3 d during which it is turned at frequent intervals to facilitate complete whey drainage. The cheese, which now has its typical shape and size, is then salted by immersion in brine (260–280 g NaCl/l at 16–17°C) for 20–24 d. During this period the cheese absorbs 15–18 g NaCl/kg and its weight decreases by 4%. During the subsequent period of ripening (12–24 months) in store rooms at 16–18°C and 85% moisture, the cheese is frequently turned. At the end of ripening the cheese is cylindrical in shape, with a slightly convex side, 0·22–0·24 m high, 0·40–0·45 m diam. weighs 35–36 kg, has 320–330 g fat/kg dry matter and a minutely granulated internal structure with small holes formed by the activity of some heterofermentative lactic acid bacteria.
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REDDY, K. ANJAN, and ELMER H. MARTH. "Microflora of Cheddar Cheese Made with Sodium Chloride, Potassium Chloride, or Mixtures of Sodium and Potassium Chloride." Journal of Food Protection 58, no. 1 (January 1, 1995): 54–61. http://dx.doi.org/10.4315/0362-028x-58.1.54.
Full textAbstract:
Cheddar cheese samples from three different split lots of cheese curd were prepared with added NaCl, KCl, or mixtures of NaCl/KCl (2:1, 1:1, 1:2 and 3:4, all on wt/wt basis) to achieve a final salt concentration of 1.5 or 1.75%. Cheeses were stored at 3 ± 1°C and their microbiological characteristics were evaluated over a 36-week ripening period. Populations of aerobic microorganisms, lactic acid bacteria, nonstarter lactic acid bacteria, aerobic spores, coliforms, and yeasts and molds in cheeses made with KCl or NaCl/KCl mixtures were not significantly (P>0.05) different from those of control cheeses made with NaCl. Staphylococcus aureus and Escherichia coli were not detected in any of the test or control cheeses.
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Shakeel-Ur-Rehman, Nana Y. Farkye, Ebenezer R. Vedamuthu, and Mary A. Drake. "A preliminary study on the effect of adding yeast extract to cheese curd on proteolysis and flavour development of reduced-fat Cheddar." Journal of Dairy Research 70, no. 1 (February 2003): 99–103. http://dx.doi.org/10.1017/s0022029902005861.
Full textAbstract:
Yeast extract was used as a nutrient for growing lactobacilli in reduced-fat Cheddar cheese as early growth of non-starter lactic acid bacteria (NSLAB) in Cheddar cheese is suppressed by pasteurization of milk and the hostile environment of the cheese. Reduced-fat Cheddar cheese was manufactured from 100 kg standardized milk on two occasions. After milling, the curd was divided into two portions, C and E. To control portion, C, salt was added at normal levels. A mixture of salt and yeast extract was added to the experimental, E. The cheeses were ripened for 7 months at 8 °C and assessed for proteolysis and NSLAB growth during ripening. Mean % moisture, fat, protein, salt and pH were 40·6, 20·5, 31·1, 1·72 and 5·22 respectively, in E cheeses, and 39·5, 20·5, 30·9, 1·68 and 5·22, respectively, in C cheese. NSLAB counts in E cheeses were 101, 103, 105 cfu/g compared with 0, 101, 104 cfu/g in C respectively, after 1, 7 and 30 d of ripening. After 60 d, cell densities of NSLAB were similar (∼106 cfu/g) in C and E cheese. Addition of yeast extract to curd affected neither the electrophoretic patterns of cheese nor its water-soluble N content during ripening. However, the total free amino acids were significantly higher in E cheese than C cheese throughout ripening, suggesting faster secondary proteolysis in the former cheeses. A 6-member trained descriptive panel evaluated the cheese at 7 months and found that the E cheeses had higher intensities of whey, fruity, sulphur, nutty, sweet and sour flavours, but had lower intensities of brothy flavours as compared to C cheeses. Also, the E cheeses were perceived to be more mature than corresponding C cheese. Results show that addition of yeast extract to cheese curd is a promising method of enhancing flavour development in ripened cheeses.
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RODRIGUEZ MEDINA, MARIA L., MARIA E. TORNADIJO, JAVIER CARBALLO, and ROBERTO MARTIN SARMIENTO. "Microbiological Study of León Raw Cow-Milk Cheese, a Spanish Craft Variety." Journal of Food Protection 58, no. 9 (September 1, 1995): 998–1006. http://dx.doi.org/10.4315/0362-028x-58.9.998.
Full textAbstract:
The levels of several microbial groups (aerobic mesophilic flora, aerobic psychrotrophic flora, lactic acid bacteria, Micrococcaceae, enterococci, Enterobacteriaceae, and molds and yeasts), and some biochemical parameters were investigated during the manufacture and ripening of four batches of León cow cheese produced from raw milk without the addition of starter cultures. The study of the microbial characteristics of this cheese constitutes the first step towards the establishment of a starter culture which would allow the making of a product both more uniform and safer from the point of view of health. The total microbial counts were high throughout the elaboration and ripening. Almost all the microbial groups reached their maximum counts in curd and afterwards dropped throughout the ripening process. The greatest drop was shown by Enterobacteriaceae, which had disappeared after 3 months of ripening. Lactic acid bacteria were the major microbial group, reaching counts similar to the total aerobic mesophilic flora at all sampling points. Lactococcus lactis subsp. lactis dominated in milk (62.5% of the isolates obtained in de Man-Rogosa-Sharpe (MRS) agar at this sampling point), curd (82.5% of the isolates obtained at this sampling point) and one-week-old cheese (85% of isolates obtained at this sampling point), while Lactobacillus casei subsp. casei was the most predominant species in eight-week-old cheese (55% of isolates obtained at this sampling point) and twelve-week-old cheese (47.5% of isolates obtained at this sampling point). According to our data, a starter suitable for the production of León cow cheese would be made up of these two species. Some species of Leuconostoc or enterococci could also be added to this starter with the aim of improving the organoleptic characteristics of the final product or to emphasize the characteristics of this variety.
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Tidona, Flavio, Salvatore Francolino, Roberta Ghiglietti, Francesco Locci, Gianluca Brusa, Marcello Alinovi, Germano Mucchetti, and Giorgio Giraffa. "Application of Recombined Milk to Produce Crescenza-Type Cheese in Laboratory-Scale Cheesemaking: Implications on Technology and Sensory Properties." Foods 9, no. 7 (July 14, 2020): 928. http://dx.doi.org/10.3390/foods9070928.
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This work evaluated the effect of recombined skimmed milk (RM), mixed in different ratios (40, 60, and 100%) with fresh cow milk, on the processing technology and quality of Crescenza, an industrial soft cheese of the Italian dairy tradition. Crescenza-type cheeses were produced at a laboratory scale, following the industrial process. Control cheese consisted of Crescenza-type cheese produced with 100% whole fresh milk. Compared to control cheese, the substitution of fresh milk with 60–100% of RM deteriorated the coagulation properties and led to a higher moisture retention, whereas, with 40% of RM, the differences were not statistically significant. Cheeses produced with any concentration of RM, although of acceptable quality, differed significantly in terms of sensory properties from control cheese. The addition of colloidal calcium phosphate, or CaCl2 together with a reduction in the size of the curd at cutting, minimized the differences in composition and sensory properties between cheeses produced with 40% RM and control cheese. This study suggested the applicability of 40% RM to obtain Crescenza-type cheese with suitable quality characteristics. The type of product, the technology, the quality, and quantity of the powders are all key factors to be taken into account for a successful application.
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SANTOS, JESÚS A., TERESA-MARÍA LÓPEZ-DÍAZ, MARÍA-CAMINO GARCÍA-FERNÁNDEZ, MARIA-LUISA GARCÍA-LÓPEZ, and ANDRÉS OTERO. "Villalón, a Fresh Ewe's Milk Spanish Cheese, as a Source of Potentially Pathogenic Aeromonas Strains." Journal of Food Protection 59, no. 12 (December 1, 1996): 1288–91. http://dx.doi.org/10.4315/0362-028x-59.12.1288.
Full textAbstract:
Presumptive aeromonads were detected in 8 batches of Villalón cheese produced in dairy plants from pasteurized ewe's milk, the mean count being 2.85 ± 0.24 log CFU/g. Only four isolates from 2 batches were confirmed as motile aeromonads and they fitted the description of Aeromonas hydrophila. During cheese making of 5 batches of farmhouse-made raw milk Villalón cheese, presumptive aeromonads decreased in numbers from 3.6 log CFU/ml in raw ewes' milk to 2.2 log CFU/g after salting, but after storage for 48 h at 4°C, multiplication occurred in all cheeses (mean count, 3.7 log CFU/g). Motile aeromonads were detected in raw ewe's milk, curd, and cheese. Identification to species' level of 38 isolates which shared the properties of A. hydrophila and A. caviae was based on the “suicide phenomenon” (22 strains were identified as A. hydrophila and 16 as A. caviae). All strains of A. caviae were β-hemolytic, showed suicidal activity, and failed to produce caseinase.
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Câmara, SP, A. Dapkevicius, C. Riquelme, RB Elias, CCG Silva, FX Malcata, and MLNE Dapkevicius. "Potential of lactic acid bacteria from Pico cheese for starter culture development." Food Science and Technology International 25, no. 4 (January 15, 2019): 303–17. http://dx.doi.org/10.1177/1082013218823129.
Full textAbstract:
Autochthonous lactic acid bacteria may provide a means of promoting the quality and safety of traditional fermented food products, in particular, artisanal cheeses. Pico cheese is an artisanal, dairy specialty of the Azores in risk of disappearing. Efforts to maintain its quality to the requirements of the modern markets are, thus, necessary. Lactic acid bacteria were isolated from artisanal Pico cheese, identified by sequencing of the 16S rRNA gene, and their potential as starter cultures was evaluated by studying their acidification ability, enzymatic activities (caseinolysis, lipolysis and API-ZYM profile), diacetyl and expolysaccharide production, autolysis, antimicrobial activity against Listeria monocytogenes ATCC 7466, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29523, Pseudomonas aeruginosa ATCC 27853 and Clostridium perfringens ATCC 8357, sensory evaluation of odour formation in milk, syneresis and firmness of the curd. Several of the studied lactic acid bacteria isolates showed interesting properties for practical application as starters in artisanal cheese production. The isolates with the highest number of positive traits and, therefore, the most promising for starter development were Lactococcus lactis ssp. lactis L1C21M1, Lactobacillus paracasei L1B1E3, Leuconostoc pseudomesenteroides L1C1E6, Lactobacillus casei L1A1E5 and L1C1E8.
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ABDALLA, O. M., G. L. CHRISTEN, and P. M. DAVIDSON. "Chemical Composition of and Listeria monocytogenesSurvival in White Pickled Cheese." Journal of Food Protection 56, no. 10 (October 1, 1993): 841–46. http://dx.doi.org/10.4315/0362-028x-56.10.841.
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White pickled cheese was made from pasteurized milk with 8% salt and preserved in the whey at 4°C. About 5.0 log10 CFU/ml cells of Listeria monocytogenes were inoculated into milk, and the survival of the pathogen was studied during the storage period. The chemical composition of the cheese was also determined. L. monocytogenes were not inhibited by 8% salt, but lactic acid bacteria were unable to grow and produce acid to inhibit L. monocytogenes. During ripening, the pH never decreased below 6.0. While fat, protein, total solids, and ash contents decreased in curd during ripening, the acidity and salt did not change. In cheese whey, fat, protein, acidity, and salt showed a slight increase, while total solids and ash contents were unchanged.
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MICHEL, VALÉRIE, and FRANK G. MARTLEY. "Streptococcus thermophilus in Cheddar cheese – production and fate of galactose." Journal of Dairy Research 68, no. 2 (May 2001): 317–25. http://dx.doi.org/10.1017/s0022029901004812.
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The behaviour of Streptococcus thermophilus in combination with Lactococcus lactis subsp. cremoris or subsp. lactis mesophilic starters in experimental Cheddar cheese is reported. In a standard manufacturing procedure employing a 38 °C cook temperature, even very low levels (0·007%) of Str. thermophilus combined with normal levels of the mesophilic starter (1·7%) resulted in increased rates of acid production, the formation of significant amounts of galactose (∼ 13 mmol/kg cheese), and populations nearly equivalent to those of the mesophilic lactic starter in the curd before salting. At a 41 °C cook temperature, the Str. thermophilus attained a higher maximum population (∼ log 8·2 colony forming units (cfu)/g) than the Lc. lactis subsp. cremoris (∼ log 6·8 cfu/g) and formed more galactose (∼ 28 mmol/kg). Lactobacillus rhamnosus, deliberately added to a cheese made using Str. thermophilus starter and which contained 24 mmol galactose/kg at day one, utilized all the galactose during the first 3 months of cheese ripening. Adventitious non-starter lactic acid bacteria had the potential to utilize this substrate too, and a close relationship was demonstrated between the increase in this flora and the disappearance of the galactose. Some possible consequences for cheese quality of using Str. thermophilus as a starter component are discussed.
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MARGOLLES, ABELARDO, ANA RODRÍGUEZ, and CLARA G. de los REYES-GAVILÁN. "Behavior of Listeria monocytogenes during the Manufacture, Ripening, and Cold Storage of Afuega'l Pitu Cheese." Journal of Food Protection 60, no. 6 (June 1, 1997): 689–93. http://dx.doi.org/10.4315/0362-028x-60.6.689.
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Afuega'l Pitu is an artisanal acid-coagulated cheese manufactured in Asturias (northern Spain) and mainly consumed between the 3rd and the 30th day of ripening. Six cheese-making trials were performed in a pilot plant by using pasteurized whole milk inoculated with Listeria monocytogenes (strain L2 [serotype 1/2a], L39, or L41 [serotype 4b]) to ca. 2.7 log CFU/ml. A starter containing three strains, Lactococcus lactis subsp. lactis IPLA 947, Lactococcus lactis subsp. lactis biovar diacetylactis IPLA 838, and Leuconostoc citreum IPLA 616, grown separately in milk and combined in the volumetric proportion 3:1:1.3 was used. During the acidification L. monocytogenes counts increased 2.78- to 7.03-fold, depending on the strain, and remained within the curd; from this time counts decreased abruptly and were not detected in cheeses beyond the 7th day. The average pH in the curd was 4.43, and it decreased to around 4.0 in 5- to 7-day-old cheeses. These pH values were near the tolerance limit for L. monocytogenes and probably caused cell damage. Although moisture, aw, and NaCl levels were not limiting for the growth and survival of L. monocytogenes, salt content must be considered as a contributing factor in L. monocytogenes inactivation. Finally, the L2 strain grew better in curd and was slightly more resistant to low pH and refrigeration than strain L39 or L41. The manufacture of Afuega'l Pitu cheese from pasteurized milk and the design of a specific starter from the autochthonous lactic microbiota can lead to a safer product that can be consumed after very short ripening periods.
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REDDY, K. ANJAN, and ELMER H. MARTH. "Lactic Acid Bacteria in Cheddar Cheese Made with Sodium Chloride, Potassium Chloride or Mixtures of the Two Salts." Journal of Food Protection 58, no. 1 (January 1, 1995): 62–69. http://dx.doi.org/10.4315/0362-028x-58.1.62.
Full textAbstract:
Three different split lots of Cheddar cheese curd were prepared with added sodium chloride (NaCl) potassium chloride (KCl) or mixtures of NaCl/KCl (2:1 1:1 1:2 and 3:4 all on wt/wt basis) to achieve a final salt concentration of 1.5 or 1.75%. At intervals during ripening at 3±1°C samples were plated with All-Purpose Tween (APT) and Lactobacillus Selection (LBS) agar. Isolates were obtained of bacteria that predominated on the agar media. In the first trial (Lactococcus lactis subsp. lactis plus L. lactis subsp. cremoris served as starter cultures) L. lactis subsp.lactis Lactobacillus casei and other lactobacilli were the predominant bacteria regardless of the salting treatment Received by the cheese. In the second trial (L. lactis subsp. lactis served as the starter culture) unclassified lactococci L. lactis subsp. lactis unclassified lactobacilli and L. casei predominated regardless of the salting treatment given the cheese. In the third trial (L. lactis subsp. cremoris served as the starter culture) unclassified lactococci unclassified lactobacilli L. casei and Pediococcus cerevisiae predominated regardless of the salting treatment applied to the cheese Thus use of KCl to replace some of the NaCl for salting cheese had no detectable effect on the kinds of lactic acid bacteria that developed in ripening Cheddar cheese.
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Dimitrovska, Gordana. "Dynamics of Active and Titratable Acidity in Bieno Cheese." International Journal of Research and Review 8, no. 6 (June 29, 2021): 232–37. http://dx.doi.org/10.52403/ijrr.20210628.
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Experimental research describes the autochthonous traditional production of bieno cheese and its importance in conditions of globalization and industrialization in food production. The purpose of this research was to present the technology of three varieties of indigenous bieno cheeses (A, B, and C) in the Mariovo region, in the Republic of North Macedonia, and to monitor the dynamics of active and titratable acidity as important parameters in the technological process. During the research, the changes of these parameters of the three variants (A, B, and C) of cheeses in different stages of maturation were monitored (in curd, cheese after dry ripening, cheese after dry salting, the 20th day of ripening, and the 45th day of ripening). The rheological properties of the cheese are greatly influenced by the active (pH) and titratable (0SH) acidity. During the ripening of cheeses under the influence of lactic acid bacteria, the biggest changes occur in lactose, which transforms lactose into lactic acid and other organic acids that play an important role in the formation of sensory and rheological characteristics of cheeses. The displayed technological parameters are of great importance for the bieno cheeses and can serve in the direction of standardization of this cheese and its protection with a designation of origin or a geographical indication based on the source technology. Keywords: Bieno cheese, technology, active and titratable acidity.
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Spyrelli, Evgenia, Anastasios Stamatiou, Chrysoula Tassou, George-John Nychas, and Agapi Doulgeraki. "Microbiological and Metagenomic Analysis to Assess the Effect of Container Material on the Microbiota of Feta Cheese during Ripening." Fermentation 6, no. 1 (January 18, 2020): 12. http://dx.doi.org/10.3390/fermentation6010012.
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The aim of the present study was to assess the influence of ripening container’s material on the bacterial diversity of Feta cheese PDO (Protected Designation of Origin). The microbiota of fresh and mature cheese produced in plastic and stainless steel container was monitored by microbial enumeration and 16s rRNA gene sequencing. According to the obtained results, lactic acid bacteria (LAB) was the dominant microbiota of fresh and mature cheese. Metagenomics data revealed that fresh cheese was dominated by Lactococcus followed by members of Enterobacteriaceae family and Pseudomonas. Similarly, Lactococcus was the most abundant genus detected in mature cheese (54 days and 120 days), regardless of the container’s material. In both fresh and mature cheese, species of Pseudomonas, Streptococcus, Acinetobacter, Lactobacillus, Flavobacterium, and Carnobacterium were detected. The abundance of Enterobacteriaceae, Moraxellaceae and Pseudomonadaceae in mature cheese ripened in stainless steel container seems to be numerically reduced after 120 days of storage compared to the cheese ripened in plastic container but not significant differences were observed (p > 0.05). In conclusion, metagenomic analysis suggests that ripening container’s material does not affect the microbial community responsible for the ripening of feta cheese PDO.
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Marcatti, Bruna, Ana Mônica Quinta Barbosa Habitante, Paulo José do Amaral Sobral, and Carmen Sílvia Favaro-Trindade. "Minas-type fresh cheese developed from buffalo milk with addition of L. acidophilus." Scientia Agricola 66, no. 4 (August 2009): 481–85. http://dx.doi.org/10.1590/s0103-90162009000400008.
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Effective incorporation of a probiotic into foods requires the culture to remain viable all along processing and storage, without adverse alterations to sensory characteristics. The objective of this work was developing Minas-type fresh cheese with probiotic properties from buffalo milk. Four batches of Minas-type fresh cheese were prepared using buffalo milk: batch T1 in which neither culture nor lactic acid added; batch T3 in which only lactic acid added; batches T2 and T4 , both added of Lactobacillus acidophilus LAC 4, but T4 was also acidified. Resulting cheeses were evaluated for probiotic culture stability, texture profile, sensory acceptance, and changes in pH. The T4 probiotic cheese presented hardness, gumminess, and chewiness significantly lower than the other treatments. However, values for springiness and cohesiveness did not differ between all cheeses, and no sensory differences (p > 0.05) were found between treatments for texture, taste, and overall acceptance. The addition of probiotic to the acidified cheese (T4) yielded best aroma. The populations of L. acidophilus were greater than 10(6) CFU g-1 after 28 days of storage all products. Minas-type fresh cheese from buffalo milk is a suitable food for the delivery of L. acidophilus, since the culture remained viable during the shelf life of the products and did not negative affect analysed parameters.
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Licitra, G., J. C. Ogier, S. Parayre, C. Pediliggieri, T. M. Carnemolla, H. Falentin, M. N. Madec, S. Carpino, and S. Lortal. "Variability of Bacterial Biofilms of the “Tina” Wood Vats Used in the Ragusano Cheese-Making Process." Applied and Environmental Microbiology 73, no. 21 (August 24, 2007): 6980–87. http://dx.doi.org/10.1128/aem.00835-07.
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ABSTRACT Ragusano cheese is a “protected denomination of origin” cheese made in the Hyblean region of Sicily from raw milk using traditional wooden tools, without starter. To explore the Ragusano bacterial ecosystem, molecular fingerprinting was conducted at different times during the ripening and biofilms from the wooden vats called “tinas” were investigated. Raw milks collected at two farm sites, one on the mountain and one at sea level, were processed to produce Ragusano cheese. Raw milk, curd before and after cooking, curd at stretching time (cheese 0 time), and cheese samples (4 and 7 months) were analyzed by PCR-temporal temperature gel electrophoresis (PCR-TTGE) and by classical enumeration microbiology. With the use of universal primers, PCR-TTGE revealed many differences between the raw milk profiles, but also notable common bands identified as Streptococcus thermophilus, Lactobacillus lactis, Lactobacillus delbrueckii, and Enterococcus faecium. After the stretching, TTGE profiles revealed three to five dominant species only through the entire process of ripening. In the biofilms of the two tinas used, one to five species were detected, S. thermophilus being predominant in both. Biofilms from five other tinas were also analyzed by PCR-TTGE, PCR-denaturating gradient gel electrophoresis, specific PCR tests, and sequencing, confirming the predominance of lactic acid bacteria (S. thermophilus, L. lactis, and L. delbrueckii subsp. lactis) and the presence of a few high-GC-content species, like coryneform bacteria. The spontaneous acidification of raw milks before and after contact with the five tinas was followed in two independent experiments. The lag period before acidification can be up to 5 h, depending on the raw milk and the specific tina, highlighting the complexity of this natural inoculation system.
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López, Pascual, Andrés Marcos, and María A. Esteban. "New equation for prediction of water activity in unripe cheese." Journal of Dairy Research 57, no. 4 (November 1990): 587–92. http://dx.doi.org/10.1017/s0022029900029630.
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SummaryThe water activity of fresh cheese made from cows', ewes' and goats' milk, as well as curd cheese and whey cheese, responds to the equation aw = 0·9719−0·0044[NaCl] + 0·0041 pH, with a square of multiple correlation coefficient R2 = 0·95. This equation was obtained by multiple regression analysis of data point sets of the variables x (g NaCl/100 g moisture), y (pH) and z (water activity, aw) determined as the average of values provided by six samples of each unripe product. Application of this equation to 30 individual samples of unripe dairy products investigated yielded calculated aw values within ±0·005 and ±0·002 aw units of the experimental water activities in 86·7% and 53·3% of the samples, respectively. These results are closer to the experimental values than those provided by earlier predictive equations.
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Weeks, Michael G., Eileen J. Gosling, and Peter A. Munro. "Heavy coagulum formation in lactic casein manufacture: solids content, pH and gel strength variations through setting lactic coagulum." Journal of Dairy Research 61, no. 1 (February 1994): 59–70. http://dx.doi.org/10.1017/s0022029900028053.
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SummarySamples of lactic coagulum were obtained from various depths in a lactic casein silo. The casein content at the base of the silo was in the range 38–62 g/kg compared with 25–30 g/kg in the bulk of the silo. This layer of higher casein concentration, called heavy coagulum, was up to l m deep and appeared long before the bulk milk had fully coagulated. It had a lower pH than the bulk, e.g. pH 4·35 when the bulk pH was 5·2. The heavy coagulum layer was removed from the silo last during pump-out. A laboratory coagulation tube was designed to permit more intensive study of heavy coagulum formation. Starter bacteria levels in the heavy coagulum were 10–160 times those in the bulk. Heavy coagulum had a visibly different curd structure (a much more granular gel), produced differently shaped extrusion curves, and had a higher gel strength than coagulum from the bulk. The heavy coagulum phenomenon had many similarities to minor sludge formation in Cottage cheese manufacture.
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Castaneda, Nuria, and Youngsoo Lee. "Microstructure of a Model Fresh Cheese and Bioaccessibility of Vitamin D3 Using In Vitro Digestion." Gels 5, no. 1 (March 10, 2019): 16. http://dx.doi.org/10.3390/gels5010016.
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In this study, the effect of a composition (protein to fat (P/F) ratio) and a processing condition (homogenization pressure for emulsification of cheese milk) on the texture, microstructure, and bioaccessibility of vitamin D3 of a model acid coagulated fresh cheese was evaluated. It was hypothesized that increasing P/F ratios (0.9, 1.3, 1.7, and 2) and homogenization pressures (17, 50, 75, and 150 MPa) will decrease the particle size of the cheese milk emulsion. The decreased emulsion particle size will result in a more rigid and elastic cheese matrix with smaller pore sizes, with an increased interfacial surface area of fat particles, which will then improve the bioaccessibility of vitamin D3. The P/F ratio exhibited a positive impact on the texture in a large deformation analysis. On the other hand, the effect of the P/F ratio and homogenization pressure was not significant on rheological properties of the cheese using a small deformation by means of a frequency sweep test, nor the porosity determined by environmental scanning electron microscopy (ESEM). These results suggested that the modification of the microstructure of acid coagulated fresh cheeses required other variables than P/F ratio and homogenization pressure probably due to a compression step after curd formation. Interestingly, the bioaccessibility of vitamin D3 measured by in vitro digestion was reduced as P/F ratio and homogenization pressure increased, which may indicate a reinforced protein–protein interaction that affected protein hydrolysis.
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Leclercq-Perlat, Marie-Noëlle, and Henry-Eric Spinnler. "The type of cheese curds determined the colouring capacity of Brevibacterium and Arthrobacter species." Journal of Dairy Research 77, no. 3 (May 13, 2010): 287–94. http://dx.doi.org/10.1017/s0022029910000245.
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This study compares the colouring capacity of Brevibacterium aurantiacum (BA), Brevibacterium BL and Arthrobacter species AS in relation to deacidified media made from lactic curd (Epoisses), mixed curds (Munster) and rennet curds (Livarot or Reblochon). BA colouring capacity proved to be constant, leading to a dark orange colour, irrespective of the deacidified media. However, it gave too dark a colour for Reblochon. The strains BL and AS were not adapted to the colouring of Epoisses deacidified medium. On the Livarot or Munster deacidified medium, these two strains provided a light yellow orange colour range that was not suitable for these cheeses. However, these two strains (BL and AS) produced a suitable colour for Reblochon deacidified medium.
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Bassi, Daniela, Simona Gazzola, Eleonora Sattin, Fabio Dal Bello, Barbara Simionati, and Pier Sandro Cocconcelli. "Lactic Acid Bacteria Adjunct Cultures Exert a Mitigation Effect against Spoilage Microbiota in Fresh Cheese." Microorganisms 8, no. 8 (August 6, 2020): 1199. http://dx.doi.org/10.3390/microorganisms8081199.
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Lactic acid bacteria (LAB) have a strong mitigation potential as adjunct cultures to inhibit undesirable bacteria in fermented foods. In fresh cheese with low salt concentration, spoilage and pathogenic bacteria can affect the shelf life with smear on the surface and packaging blowing. In this work, we studied the spoilage microbiota of an Italian fresh cheese to find tailor-made protective cultures for its shelf life improvement. On 14-tested LAB, three of them, namely Lacticaseibacillus rhamnosus LRH05, Latilactobacillus sakei LSK04, and Carnobacterium maltaromaticum CNB06 were the most effective in inhibiting Gram-negative bacteria. These cultures were assessed by the cultivation-dependent and DNA metabarcoding approach using in vitro experiments and industrial trials. Soft cheese with and without adjunct cultures were prepared and stored at 8 and 14 °C until the end of the shelf life in modified atmosphere packaging. Data demonstrated that the use of adjunct cultures reduce and/or modulate the growth of spoilage microbiota at both temperatures. Particularly, during industrial experiments, C. maltaromaticum CNB06 and Lcb. rhamnosus RH05 lowered psychrotrophic bacteria of almost 3 Log CFU/g in a 5-week stored cheese. On the contrary, Llb. sakei LSK04 was able to colonize the cheese but it was not a good candidate for its inhibition capacity. The combined approach applied in this work allowed to evaluate the protective potential of LAB strains against Gram-negative communities.
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Coelho, M. C., C. C. G. Silva, S. C. Ribeiro, M. L. N. E. Dapkevicius, and H. J. D. Rosa. "Control of Listeria monocytogenes in fresh cheese using protective lactic acid bacteria." International Journal of Food Microbiology 191 (November 2014): 53–59. http://dx.doi.org/10.1016/j.ijfoodmicro.2014.08.029.
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Novella-Rodríguez, Sonia, M. Teresa Veciana-Nogués, Artur X. Roig-Sagués, Antonio J. Trujillo-Mesa, and M. Carmen Vidal-Carou. "Evaluation of biogenic amines and microbial counts throughout the ripening of goat cheeses from pasteurized and raw milk." Journal of Dairy Research 71, no. 2 (May 2004): 245–52. http://dx.doi.org/10.1017/s0022029904000147.
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The effect of the hygienic quality of milk on changes in microbial counts and biogenic amine content was evaluated during ripening of goat cheeses manufactured from pasteurized and raw milks at 1, 14, 30, 60 and 90 d. The original milk, rennet, curd and whey were also included in the study. The pH, salt content and extent of proteolysis in the cheese were also evaluated. Spermidine and spermine were the main amines in raw milk, while they were minor amines in cheeses. Other amines increased markedly during ripening, tyramine being the main amine in cheese made from raw milk and cadaverine and putrescine in those produced from pasteurized milk. Enterobacteriaceae counts decreased during ripening whereas those of lactic acid bacteria increased, especially lactobacilli and enterococci. Cheese made from raw milk showed higher microbial counts during ripening than those made from pasteurized milk, especially for Enterobacteriaceae and enterococci, counts being 2 or 3 log units higher. Raw milk cheese showed remarkably higher biogenic amines compared with pasteurized milk cheeses. Therefore, pasteurization of milk causes a decrease in final biogenic amine content of cheese as a result of the reduction of its microbial counts.
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Dang, Bich Phuong, Alan F. Wolfschoon Pombo, and Ulrich Kulozik. "Physicochemical changes during the creaming reaction in acid curd fresh cheese: Water mobility and forced synaeresis." International Journal of Dairy Technology 72, no. 2 (February 17, 2019): 295–302. http://dx.doi.org/10.1111/1471-0307.12585.
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Pintado, M. E., A. C. Macedo, and F. X. Malcata. "Review: Technology, Chemistry and Microbiology of Whey Cheeses." Food Science and Technology International 7, no. 2 (April 2001): 105–16. http://dx.doi.org/10.1177/108201320100700202.
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In whey cheese manufacture, whey, plain or added with milk, is heated by direct fire, bubbling steam or alternatively in jacketed vats. In some cases, salt s or organic acids are previously added. At 80-85 OC, the first particles of curd form; at 85-95 'C, the curd may be cooked for a few minutes to reduce moisture content and/or to obtain the desirable level of browning. After drainage at room temperature during molding for ca. 4 h, whey cheese is stored at ca. 4 'C. The typical mass yield is 6%, but addition of milk, calcium salts and preliminary concentration of protein (by condensation or ultrafiltration techniques) may increase yield considerably. Some types of whey cheeses are supposed to be consumed within a short time upon manufacture (e.g., Ricotta, Requeijdo and Manouri), whereas others bear a longer shelf life (e.g., Gjetost, Mysost and Myzithra). Whey cheeses are significantly different from one another in terms of chemical composition, which is mainly due to variations in the source and type of whey, as well as to the processing practices followed. Moisture content and pH of whey cheeses are usually high and favor microorganism growth (molds, yeasts, lactic acid bacteria and Enterobacteriaceae account for the dominant microflora in these cheeses). Adequate packaging of whey cheeses should be provided, and legislation should be prepared to fix standard characteristics of each type of whey cheese, and hence protect typical products from adulteration and fakes. Marketing efforts should also be aimed at increasing whey cheese consumption, either directly or incorporated in desserts, snack dips and pasta-type dishes.