Journal articles on the topic 'Francisco and Jacobus (Firm)'

Resumen: El presente artículo analiza, en la primera parte, la presencia del copista griego Jacobo Diasorino en Italia, en una primera estancia de juventud y en una posible segunda estancia hacia 1550, desde el punto de vista de las copias manuscritas que pudo haber ejecutado en esos momentos y de su contexto. En la segunda, se centra en aquellos de sus manuscritos que acabaron entrando la Real Biblioteca del monasterio de San Lorenzo de El Escorial, con especial atención al fondo del humanista italiano Francisco Patrizi.Palabras clave: Jacobo Diasorino, Francisco Patrizi, El Escorial, Italia, Henri Estienne, siglo XVI, copistas griegos.Abstract: This article analyses, in the first part, the presence of the Greek scribe Jacobos Diassorinos in Italy during his youth and possibly around 1550. Both stays are examined from the point of view and the context of the copies he could have done in such moments. In the second one, the article focusses on those of his manuscritps kept in the Real Biblioteca of the monastery of San Lorenzo de el Escorial (Spain), especially on those which belonged to the Italian humanist Francesco Patrizi.Keywords: Jacobos Diassorinos, Francesco Patrizi, El Escorial, Italy, Henri Estienne, 16th century, Greek scribes.

One of the most important influences on the development of Cherríe Moraga's feminist theatre was undoubtedly the work of Maria Irene Fornes, the Cuban American playwright and director. Moraga wrote the first drafts of her second play Shadow of a Man while on Fornes's residency programme at the INTAR Hispanic Playwrights-in-Residence Laboratory in New York, and later Fornes directed the premiere at the Brava-Eureka Theatre in San Francisco (1990). The play radically restages the Chicana body through an exploration of the sexual and gendered politics of the family. Much has been written on how the family has traditionally been the stronghold of Chicana/o culture, but Shadow of a Man stages one of its most powerful criticisms, revealing how the complex kinship structures often mask male violence and sexual abuse. Using archival material and a range of critical studies, in this article Elizabeth Jacobs explores Moraga's theatre as an embodied feminist practice and as a means to displace the entrenched ideology of the family. An earlier version of this article was presented at the Department of English and Creative Writing, Aberystwyth University, as part of the 2014 International Women's Day events. Elizabeth Jacobs is the author of Mexican American Literature: the Politics of Identity (Routledge, 2006). Her articles have appeared in Comparative American Studies (2012), Journal of Adaptation and Film Studies (2009), Theatres of Thought: Theatre, Performance, and Philosophy (2008), and New Theatre Quarterly (2007). She works at Aberystwyth University.

Book review by Michele K. Masterfano. Flamholtz, Eric G. and Yvonne Randle. Growing Pains: Transitioning from an Entrepreneurship to a Professionally Managed Firm. San Francisco: Jossey-Bass, 2007. ISBN 9780787986162

Review, selection and transcription of part of the trading correspondence of the firm Ventoso, suscribed almost entirely by Francisco Gervasio Ventoso Romero (1797-1856). Through the reading of the letters we make an interesting approach to the existing trading practice on the first half of the XIXth century in the Canary Islands as well to the human and business network linked to the economical field in the Archipelago, Peninsula, England and Cuba.

The results of a study of earthquake peak horizontal component acceleration hazard is presented for the nine counties of the San Francisco Bay Area. The main objective of the study is to develop an interim and simple estimate of the regional earthquake acceleration hazard using an extended hazard algorithm that allows a more complete consideration of recently published regional earthquake source parameters. Complexities of source directivity, soft foundation conditions, and site topography are not considered. Results are presented in the form of contour maps of acceleration on rock or stiff soil with a probability of exceedance of 10% for the next (as of 1990) 20 and 50 years. As would be expected, the locations of greatest earthquake shaking hazard in the Bay Area are near major active faults. When characteristic earthquake size, characteristic earthquake recurrence interval, and time of occurrence of the last earthquake along specified fault segments (if known) are explicitly used in the source model for larger earthquakes, the hazard computed depends significantly on these parameters. If smaller earthquakes are not constrained to major faults, but are considered to occur randomly throughout the Bay Area, significant high-frequency motion hazard at points away from the immediate vicinity of active faults comes from these smaller, randomly located earthquakes. All results show considerable variation of peak accelerations over areas generally treated as homogeneous in conventional engineering design practice for seismically active regions.

Regional economies are shaped by their economic histories and existing endowments. This paper explores the question: how do a region’s economic history and institutional endowments affect its success and trajectory in an emerging industry? Our case, electric vehicle development and production, is an industry which combines more traditional skilled manufacturing with knowledge-driven innovation activities. We present deep qualitative case studies of two regions, focusing on one firm in each. The case of Tesla in the San Francisco Bay Area examines an electric vehicle firm in a region with a strong tech innovation system, while the case of Maserati in Emilia-Romagna, Italy, examines a firm that builds on a regional history of automotive manufacturing. Across cases, we compare regional skill endowments, institutional coordination, and place-based policymaking. We conclude that, as an emerging industry under a new economic paradigm, electric vehicle manufacturing by Tesla and Maserati represents two different conceptions of the industry and consequently two different location strategies. Yet these two strategies remain rooted in regional contexts, owing both their success to successful exploitations of these, and their struggles to their failure to compensate for regional gaps. This presents a clear opportunity for place-based industrial strategy to evolve and intervene.

Sausalito, CA—Come dinner time aboard his yacht Adequate Award, and Melvin Belli really knows how to put on the dog. Tonight, his dining-room table is set with bone china and sterling silver, and browsing among the tableware is one hungry Italian greyhound. I know the animal is hungry because he has walked across the table and taken a bite of my bread plate. . . There are those who would have you believe that Mr. Belli, the King of Torts, has become so eccentric that more than dinner is becoming chaotic. . . Since summer, the 81-year-old lawyer has been barred by court order from his 25-room San Francisco mansion. His wife got the order after filing a petition for legal separation. She complained at the time to reporters and police that Mr. Belli physically and verbally abused her and their teenage daughter. Even more bizarre, she claims that he falsely accused her of having sex with a number of family friends, including celebrities of both sexes. . . Another shot fired across the bow: In a lawsuit pending in Tax Court, the Internal Revenue Service contends that Mr. Belli, in effect, back-dated documents to avoid paying gift taxes in a transaction involving his San Francisco law-office building. . . His firm, Law Offices of Melvin Belli, Sr., is on trial, too, these days. Three years ago, Mr. Belli lost a malpractice case resulting in a $3.8 million judgement against him. Since then, six more malpractice suits have been filed against him in San Francisco Superior Court. . .

The Balaia Hotel (1964–1968) near Albufeira, in the Algarve, designed by Francisco Conceição Silva and Maurício de Vasconcellos, is one of the first and most iconic seaside holiday megastructures built in Portugal. In its territorial approach, physical dimension and range of amenities, it represents a new stage of development in seaside tourism accommodation facilities. A self-contained and self-sufficient structure that shapes its own landscape. Also, the “turn key” commission implied the transition from the small-scale architecture office to the multidisciplinary design and planning firm, revolutionizing the Portuguese architectural culture and professional practice. This essay addresses the impact of the Balaia Hotel on the traditional structures of architecture production and, consequently, on the disciplinary debate of the 1960s in Portugal.

On January 18, 1995, the University of California convened a special meeting to discuss the business operations of its five academic medical centers in San Francisco, Sacramento, San Diego, Los Angeles, and Irvine. Because of the rapid developments occurring in the maturing and competitive managed care market in California, the academic medical centers are facing unprecedented financial pressures. Charles Townsend, of the accounting firm KPMG Peat Marwick, stated that the medical centers' staff would need to be cut by at least 2500 full-time equivalents, including physicians and nurses, by the year 1999. William Kerr, Director of the University of California San Francisco Medical Center, forecasted a comprehensive restructuring and streamlining of services. Jordan Cohen, president of the Association of American Medical Colleges, described the challenges facing these five academic medical centers and others like them as "truly seismic."1 The rise of managed care medical systems during the last 5 years has led many researchers to question whether the academic medical center will survive in its current state.2-6 Market forces are changing the provision of medical care at an extremely fast pace. By 1998, an estimated 60% of people living in US cities will be covered by managed care health plans. Fewer hospital admissions, shorter hospital stays, and decreased reimbursements associated with managed care have decreased hospitals' operating gains. Academic medical centers, such as those in California, are facing pressure to lower health care delivery costs. The probable decreases in Medicare and research funds under the current Congress also threaten the financial revenues of academic medical centers.

Chronic traffic congestion is widely assumed to negatively affect regional economic performance, but this assumption has been only lightly tested. We examine the traffic congestion–economic performance link using data for the San Francisco Bay Area and find that the effect of traffic on the regional economy may be both less significant and more nuanced than is widely assumed. Our analysis examines how traffic congestion affects the location of new business establishments in six industries: advertising, biotechnology, computer systems design, information technology manufacturing, securities, and, as a control, groceries & supermarkets. New business establishments are a key driver of economic performance because they account for the majority of job creation in the USA. We find little evidence that traffic levels affect the location of new establishments in the Bay Area, and when we do observe an effect it is a positive one; that is, after controlling for a wide array of factors known to influence firm location, new firms are often more likely to start up in already congested areas. This does not mean that traffic congestion attracts new firms, but instead that the access advantages new firms accrue from clustering near same-industry firms strongly outweigh the added impedance of traffic congestion in these built-up areas of agglomeration.

Celem opracowania jest określenie poziomu odporności funkcji kontrolno-zarządczych na kryzys dominującego sektora kreującego tę funkcję w miastach Stanów Zjednoczonych oraz prognoza ich odporności do roku 2025. W tym celu posłużono się wnioskowaniem w oparciu o trend zmian wskaźnika światowych centrów ekonomicznych (ŚCE). Wskaźnik obliczony został na podstawie standaryzowanych wartości wyników finansowych korporacji zlokalizowanych na obszarze metropolitalnym z uwzględnieniem jego potencjału ekonomicznego oraz zróżnicowania sektorowego. W latach 2006–2016 w większości amerykańskich światowych centrów ekonomicznych spadła liczba siedzib zarządów, ale jednocześnie wzrosła ich odporność na kryzys głównego sektora kreującego funkcję kontrolno-zarządczą, a najważniejszymi ŚCE w USA są obecnie Nowy Jork, Chicago, Houston i San Jose. Wykazano, iż odporność miast USA na kryzys funkcji kontrolno-zarządczej wzrastała później niż wartość rynkowa firm po spowolnieniu gospodarczym z 2008 roku. Z kolei w 2025 roku najważniejszymi ŚCE w Stanach Zjednoczonych będą Nowy Jork, Chicago, San Jose i San Francisco. Nie zauważono zależności przestrzennej w prognozowanej dynamice wartości wskaźnika ŚCE. Spadki i wzrosty wartości występują mniej więcej równomiernie na przestrzeni całego kraju. Nie widać też wpływu konkretnego sektora na dynamikę analizowanego wskaźnika.

The classic, originating in the intellectual development of Greek and Rome, has consistently been an essential subject of the Western culture, occupying an important role as well in Germanic law and Christian religion with its highlight on the spirit of reason, logic and humanity even in the Middles. With the discovery of Greece and Roman antiquity in Italian in the Renaissance, the classical mythologies and the masterpieces by Aristotle and Plato were once again emphasized, and interpreted from a different perspective. Hundreds of years later, the Enlightenment thinkers and neo-classical artists extracted the spirit of reason and morality from the classic. The neo-classical artists criticized the frivolous Rococo, choosing serious subjects aiming to elevate the morality of human, focusing on the integrity of the painting, strengthening sketch than color, and in this way restoring and revoking the Greco-Roman classical spirit. Yet the turmoil of the French Revolution and the Napoleonic war for decades doubts the limitation of the reason, revealing the madness of discipline and organization, displaying the mechanized and the alienated will of human-being. Delacroix and Goya, the representatives of romanticism artists, have been considered the rebels of neo-classic and the academy and the pioneers of modernity. Their works show the might of passion and emotion, depicting the fear, numbness, and indifference of human-being facing death, contrasting with the dignity and the firm attitude expressed by the neo-classical figures. This modernity roots in a certain historical context of the classic. Through pictorial analysis and the study of Delacroix's diaries and historical materials in contrast to the Baroque and Neo-classical artists to show Goya and Delacroix spontaneously invoke and reconstruct the spirit of ancient Greco-Roman art.

PrécisThe authors assume that costs can no longer be contained in the United States health care system and that the present system cannot be sustained beyond the near future. Three of the authors are affiliated with an applied economic research and consulting firm, and the fourth is president of the Healthcare Financial Management Association. They are trained in business and city planning. The bibliography lists articles from such journals as Hospitals, Business & Health, Business Insurance, and Medical Economics.The book is directed to members of hospital governing boards and other hospital administrators, but it will be of interest to students of health policy. Part I highlights tensions between what the authors call the worlds of doctors and hospital administrators struggling to survive, on the one hand, and health care planners worried about spiraling costs on the other. Part II contains five chapters that suggest reasons for growing costs and that criticize cost shifting as a remedy.In Part III the authors evaluate alternative health care systems by presenting four future scenarios: incremental change, universal access, consumer choice model, and single payer system. In these chapters they also approach the fundamental purpose of the book—to “help physicians, hospitals, and health plans take the next steps to position themselves for the future.” While not highly analytical, the book is a readable and thoughtful supplement to more abstract critiques of the impact of today's health care system on distributive justice.

Early modern connections between the Pacific shores of the Spanish Empire have been assessed in previous studies and yet, studies on the subsequent developments during the late eighteenth and early nineteenth centuries are scant. This particularly applies to the consequences of the commercial developments at the end of the Manila Galleon and the collapse of the Spanish Empire in America. Through the analysis of the professional networks of Francisco Xavier de Ezpeleta, and Juan Nepomuceno Machado, which stretched from Asia to Mexico at the time, this paper proposes some preliminary conclusions about trans-Pacific links. It focuses on the study of networks and connections, and builds upon archival sources — mostly from the Jardine Matheson Archive, in Cambridge. In the transition from the eighteenth to the nineteenth centuries, actors who made up Hispanic trading networks in what remained of the crumbling Spanish Empire, reorganised to benefit from opportunities as mercantilist limitations came to an end. In so doing, by the 1820s, Hispanic traders allied with the forebears of the British firm of Jardine, Matheson & Co., and controlled the resulting networks. This paper points to Hispanic trans-Pacific connections during the development of European private trade between East Asia and the Mexican Pacific. Ties between British and Hispanic merchants are key for the analysis of the opium trade and Western imperialism in East Asia, and the development of British commercial hegemony in Latin America in the nineteenth century.

Abstract Background: The cause and continuation of vaso-occlusion in sickle cell disease (SCD) are fueled by the sickle-Red Blood Cells interactions with multiple other cell populations, promoting inflammation, obstructing the vasculature, and injuring the endothelium, leading to broad manifestations that affect most vital organs. Recent studies have identified multiple cellular components and molecular factors that contribute to the pathophysiology of SCD as reviewed by Zhang et al 2016 in Blood. It is likely that a multi-targeted approach for addressing SCD vaso-occlusion will be required to achieve the best clinical outcome. Sevuparin (DF02), a novel drug in Phase 2 for acute treatment of vaso-occlusive crisis in SCD (NCT02515838), is a polysaccharide blocking abnormal adhesion and thereby normalizing obstructed blood flow. In vitro and in vivo studies have shown potent anti-adhesive effects with a multimodal mechanism of action. In this study, we evaluate the effects of sevuparin on the adhesion of sickle whole blood from individual patients to endothelial cells (HUVECs) and vascular cell adhesion molecule-1 (VCAM-1) (Flow Firm Adhesion) and sickle-leukocyte rolling adhesion on L-selectin (Flow Dynamic Adhesion) using a standardized microfluidic flow-based adhesion assay. Methods: Blood was obtained from homozygous SCD patients (n = 12, age range 15-25yrs) in sodium citrate after obtaining informed consent. A comprehensive assessment of the effect of sevuparin on whole blood adhesive properties during simulated blood flow was assessed using standardized Flow Firm Adhesion and Flow Dynamic Adhesion assays (Functional Fluidics, Detroit MI). Flow Firm Adhesion: Whole blood firm adhesion was measured during physiologic flow in microfluidic channels (Fluxion-Bioflux 1000, San Francisco, CA) coated with either VCAM-1 or cultured HUVECs. HUVECs were activated by TNF-alpha (25ng/mL x 24 hrs.) and Histamine (100mM x 10min) prior to the assay. Whole blood was treated with increasing doses of sevuparin (0, 3, 7, 21, 200µg/mL) for 30 min. Dose response of whole blood adhesion index (cells/mm2) to sevuparin was measured. Flow Dynamic Adhesion: Rolling adhesion of isolated sickle-leukocytes on an L-selectin coated microfluidic channel was measured during physiologic flow. Isolated sickle-leukocytes were treated with increasing doses of sevuparin (0, 3, 7, 21, 200µg/mL) for 30 min. Dose response of rolling cell density (cells/mm2), rolling cell percentage (%), and average rolling velocity (µm/s) to sevuparin was assessed. Cell identification and tracking of rolling were digitally analyzed. Results:Statistically significant inhibition of sickle whole blood adhesion to HUVECs was observed at 3.0 µg/mL of sevuparin (p<0.001). In the same manner, statistically significant inhibition of sickle whole blood adhesion to VCAM-1 was observed at 200 µg/mL of sevuparin (p=0.033, absolute adhesion, p=0.001, % baseline adhesion). Each patient sample demonstrated a reduction in adhesion. Sevuparin also demonstrated a statistically significant dose-dependent reduction of sickle leukocyte rolling cell density (cells/mm2), rolling cell percentage (%), and an increase in average rolling velocity (µm/s) on L-selectin. Patient-to-patient variability in sevuparin response was observed. Conclusions: Sevuparin blocks both sickle whole blood and isolated sickle-leukocyte adhesive interactions under physiologic flow at clinically relevant concentrations. The blocking of adhesion to VCAM-1 indicates that sevuparin acts in the same manner as other heparinoids in vitro, and block the interaction with VLA-4. L-selectin is another possible target for sevuparin therapy now confirmed at the cellular level. Clinically, Okpala et al 2002 has shown that L-selectin expression by monocytes is increased in vaso-occlusive crises, compared to steady state and that both mononuclear cell and neutrophil L-selectin expression is also higher in patients with certain complications of SCD. Here we show that sevuparin acts in a multicellular manner, blocking both SS-RBC firm adhesion and L-selectin-mediated rolling adhesion of sickle-leukocytes, as well as functionally interacting with yet another key adhesion receptor VCAM-1. This further adds to sevuparin's multimodal action and its potential clinical benefits in treating the complex mechanisms manifested in vaso-occlusion and complications in SCD. Disclosures Lindgren: Dilaforette AB: Employment. White:Functional Fluidics: Employment, Equity Ownership. Liu:Functional Fluidics: Employment, Equity Ownership. Jendeberg:Dilaforette AB: Employment. Hines:Fucntional Fluidics: Employment, Equity Ownership.

This study aims to determine how the song nocturne in e-flat major op 9 no 2 by Fredrich Chopin is technically expressed through the classical guitar playing by Francisco Tarrega. This research is descriptive analysis, which in this case describes, outlines and describes the technique of playing nocturne in e-flat major op 9 no 2 which is played for classical guitar instruments. The object of this research is the classical guitar playing technique in nocturne in e-flat major op 9 no 2 by Frederich Chopin. The research instrument is the researcher himself is assisted by several equipment to process the data. The type of data used is primary data and secondary data. The steps to analyze the data were done by using interview, observation and documentation techniques. The data are organized and interpreted as finding material to answer research problems. The results showed that the techniques used in the classical guitar song nocturne in e-flat major op 9 no 2, namely arpeggio, legato, slur, glissando, barre, harmonic, appoggiatura, and trill. Tarrega replaced techniques that can also be played on guitar such as upper mordent, group peto, and staccato with slur techniques, and glissando, then condensed the rhythmic tone with a 1/32 note. The substitution of this technique is due to organological reasons, where the piano has a soft pedal and a damper pedal that can maintain the tone, while this is not possible on a guitar. The difference in the use of playing techniques or more precisely the adjustment of the playing technique of this work using classical guitar gives different nuances to its expression, namely sharp, deep, sad, mellow, firm, fast, free, bright, broad nuances on the piano. While the guitar creates a soft, gentle, broad, crowded, fast feel.

Abstract Corporate research and development (R&D) activities have long been highly concentrated in a handful of world cities. This is due to the fact that these cities (e.g., Tokyo, New York, London, and Paris) are home to the largest and most powerful transnational corporations and are globally important sites for innovative start-up firms that operate in the fastest growing industries. However, in tandem with the rapid technological changes of our age, corporate R&D activities have shifted towards newly emerging and now globally significant R&D centres, like San Jose, San Francisco, and Boston in the United States, and Beijing, Seoul, and Shenzhen in East Asia. In this paper, I will conduct a bibliometric analysis to define which cities are centres of corporate R&D activities, how different industries influence their performance, and what spatial tendencies characterise the period from 1980 to 2014. The bibliometric analysis is based upon an assumption that implies there is a close connection between the number of scientific articles published by a given firm and the volume of its R&D activity. Results show that firms headquartered in Tokyo, New York, London, and Paris published the largest combined number of scientific articles in the period from 1980 to 2014, but that the growth rate of the annual output of scientific articles was much greater in Boston, San Jose, Beijing, and Seoul, as well as some Taiwanese cities. Furthermore, it can also be seen that those cities that have the largest number of articles; i.e., that can be considered as the most significant sites of corporate R&D in which firms operate in fast-growing industries, are primarily in the pharmaceutical and information technology industries. For these reasons, some mid-sized cities that are home to globally significant pharmaceutical or information technology firms are also top corporate R&D hubs.

Purpose Graphic novels have a concept known as the origin story. The origin story is background information on how a hero or villain came into being. The purpose of this paper is to explore the origin story of corporate social responsibility at British American Tobacco (BAT). The CSR origin story is unpacked by examining corporate documents from BAT that discuss the initial development of the company’s CSR program. The BAT documents are part of the Legacy Tobacco Documents Library (LTDL), a searchable, digital archive developed and managed by the University of California, San Francisco. It contains 85,569,326 pages in 14,360,422 documents. The library was created as part of the tobacco company settlement of a major law suit in the USA. Design/methodology/approach For this case study, the authors searched the archive for documents from BAT that had the key words “corporate social responsibility.” The documents were then analyzed using qualitative content analysis to identify key themes related to BAT’s created of its CSR programs. Findings The two dominant themes were business case BAT made for CSR and the environmental factors that shaped CSR. The business case had sub-themes of the new operating environment and reinforcing employees. The environmental sub-themes were the importance of NGOS and the top issues to be addressed in CSR efforts. The themes helped to explain why BAT was engaging in CSR, the factors shaping the start of its CSR programs, and the issues it intended to address through CSR. Research limitations/implications The analysis is limited to one organization and does not include interviews to go with the archived documents. Practical implications The paper considers the implications of the analysis for theory and practice for internal CSR communication. Originality/value The documents provide a rare glimpse inside a corporate decision to begin a CSR program and how the managers “talked” about CSR. Instead of examining external CSR communication, it examines the early days of internal CSR communication at a specific firm. The yields of the document analysis provide insights into how BAT conceptualized CSR and communicated the rationale for creating a CSR program internally. Research has relied primarily upon speculation of corporate motives or corporate public discourse designed to frame their CSR efforts. The internal documents provide an unfiltered examination of the motives for a CSR program. This allows us to better understand why a CSR program was created including the motives, targets, and desired outcomes.

Background Long-distance heavy goods vehicle drivers are exposed to a multitude of risk factors associated with their occupation. The working environment of heavy goods vehicle drivers provides limited opportunities for a healthy lifestyle, and, consequently, heavy goods vehicle drivers exhibit higher than nationally representative rates of obesity and obesity-related comorbidities, and are underserved in terms of health promotion initiatives. Objective The aim of this trial was to test the effectiveness and cost-effectiveness of the multicomponent Structured Health Intervention For Truckers (SHIFT) programme, compared with usual care, at both 6 months and 16–18 months. Design A two-arm cluster randomised controlled trial, including a cost-effectiveness analysis and process evaluation. Setting Transport depots throughout the Midlands region of the UK. Participants Heavy goods vehicle drivers. Intervention The 6-month SHIFT programme included a group-based interactive 6-hour education session, health coach support and equipment provision [including a Fitbit® (Fitbit Inc., San Francisco, CA, US) and resistance bands/balls to facilitate a ‘cab workout’]. Clusters were randomised following baseline measurements to either the SHIFT arm or the control arm. Main outcome measures Outcome measures were assessed at baseline, with follow-up assessments occurring at both 6 months and 16–18 months. The primary outcome was device-measured physical activity, expressed as mean steps per day, at 6-month follow-up. Secondary outcomes included device-measured sitting, standing, stepping, physical activity and sleep time (on any day, workdays and non-workdays), along with adiposity, biochemical measures, diet, blood pressure, psychophysiological reactivity, cognitive function, functional fitness, mental well-being, musculoskeletal symptoms and work-related psychosocial variables. Cost-effectiveness and process evaluation data were collected. Results A total of 382 participants (mean ± standard deviation age: 48.4 ± 9.4 years; mean ± standard deviation body mass index: 30.4 kg/m2 ± 5.1 kg/m2; 99% male) were recruited across 25 clusters. Participants were randomised (at the cluster level) to either the SHIFT arm (12 clusters, n = 183) or the control arm (13 clusters, n = 199). At 6 months, 209 (54.7%) participants provided primary outcome data. Significant differences in mean daily steps were found between arms, with participants in the SHIFT arm accumulating 1008 more steps per day than participants in the control arm (95% confidence interval 145 to 1871 steps; p = 0.022), which was largely driven by the maintenance of physical activity levels in the SHIFT arm and a decline in physical activity levels in the control arm. Favourable differences at 6 months were also seen in the SHIFT arm, relative to the control arm, in time spent sitting, standing and stepping, and time in moderate or vigorous activity. No differences between arms were observed at 16–18 months’ follow-up. No differences were observed between arms in the other secondary outcomes at either follow-up (i.e. 6 months and 16–18 months). The process evaluation demonstrated that the intervention was well received by participants and that the intervention reportedly had a positive impact on their health behaviours. The average total cost of delivering the SHIFT programme was £369.57 per driver, and resulting quality-adjusted life-years were similar across trial arms (SHIFT arm: 1.22, 95% confidence interval 1.19 to 1.25; control arm: 1.25, 95% confidence interval 1.22 to 1.27). Limitations A higher (31.4%) than anticipated loss to follow-up was experienced at 6 months, with fewer (54.7%) participants providing valid primary outcome data at 6 months. The COVID-19 pandemic presents a major confounding factor, which limits our ability to draw firm conclusions regarding the sustainability of the SHIFT programme. Conclusion The SHIFT programme had a degree of success in positively impacting physical activity levels and reducing sitting time in heavy goods vehicle drivers at 6-months; however, these differences were not maintained at 16–18 months. Future work Further work involving stakeholder engagement is needed to refine the content of the programme, based on current findings, followed by the translation of the SHIFT programme into a scalable driver training resource. Trial registration This trial is registered as ISRCTN10483894. Funding This project was funded by the National Institute for Health and Care Research (NIHR) Public Health Research programme and will be published in full in Public Health Research; Vol. 10, No. 12. See the NIHR Journals Library website for further project information.

Dear Editor, I was exposed to a COVID-19 positive cardiologist last March 1. I had ENT clinics until March 11, treating mostly patients with complaints of cough and fever (sinusitis and bronchitis). I felt that my facial mask, hooded magnifier lens, and gloves gave me enough protection. I was wrong. They were not sufficient. I had a temperature of 38°C on March 13 and went home immediately to self-isolate. By March 15, I was coughing unremittingly and persistently as if a feather was stuck in my throat. I had no phlegm. I had no running nose, nor respiratory difficulty. But my rib muscles ached continuously - an intense, miserable pain not relieved by any position. I felt a severe point tenderness over both lower back ribs that even soft pillows could not diminish. I lacked sleep. I felt weaker as days went by. My taste was flat as I swallowed soups and arroz caldo but I still had my sense of smell. On March 16, I took Clarithromycin 500 mg and N-Acetylcysteine 600 mg, both twice daily to treat what I diagnosed as acute pharyngitis. Two more days of severe coughing, fever and sore throat made me worry about COVID-19. How come I was not getting better? In fact, I was getting BITTER over this uncertainty of COVID-19 and the treatment I had prescribed myself. I had been religiously taking probiotics to imbue me with immunity for infections like these.1 I believed that lactobacillus acidophilus, the friendly gut bacteria, stimulates the Gut Associated Lymphoid Tissue (GALT) to produce antibodies against virus and bacteria shedding into small intestines and against bacteria abnormally multiplying in the large intestine.2 I was assured by the research of Russian Dr. Elie Metchnikoff on the potent lactobacillus in yogurt (which comprised almost 50% of the Bulgarian diet and made them strong and healthy). Dr. Metchnikoff (who had won the 1908 Nobel Prize in Physiology and Medicine) honored Bulgaria by naming his friendly bacteria lactobacillus bulgaricus.3,4 Dr. Metchnikoff was later honored as the “father of natural immunity.”5 Then came the Spanish flu of 1918-1919 that killed more than 2.5 million Europeans, mostly Italians and British.6 Yet the number of those killed in Bulgaria was as close to that in Switzerland, which was the lowest. Now, the COVID-19 pandemic marched into 2020 killing 4,633 patients out of 82,918 COVID+ in Wuhan, China; 31,855 out of 219,183 COVID+ in Great Britain; 30,560 out of 219,070 COVID+ in Italy; and 80,787 out of 1,367,638 COVID+ in the USA. Ninety-one died of COVID out of 1,965 positive for COVID-19 in Bulgaria.7 I was confident that the lactobacillus acidophilus 20 billion Colony Forming Units (CFU) were stimulating production of the IgG and IgM (from GALT which produces 70% of the body’s immune globulins) needed to neutralize viruses or bacteria.8 The acidophilus produces Vitamin B specially Vit B129 which I believe made for my stronger body. I had prepared myself as I prepared my patients for the flu by consuming Vit C and Zinc. Zinc stimulates the thymus to increase immune responses to viruses.10,11 I was fortified with 2 Colostrum pills daily, preformed sources of IgG and IgA.12 I followed my regimen for acute rhinitis (though there was no nasal obstruction) which meant doing nasal SALINE washing or sprays thrice a day. I knew that the flu virus (or even the SARS-COV2) hides EARLY in the nose and sinuses and is able to produce toxins which inflame the whole body. Worse for SARS-COV2 because these drop into the tonsils and into the lungs. The nasal sprays were meant to reduce the virus numbers (viral load) in the nose and sinuses so there were less shedding. Saline washes decongest the nose to improve breathing. The 60 seconds antiseptic mouthwash followed a regimen of brushing the teeth then the palate and the tonsils and to the base of the tongue. This was to extinguish any virus lurking to go down into the lungs or GIT. This regimen was routine at 3x a day. The fever dropped slowly. Coughing diminished though the muscles constantly ached after 3 days of Clarithromycin. I was determined to have the COVID tests and a high resolution CT scan of the chest. On March 20 at the hospital ER, I explained I was a patient requesting a CBC, a COVID test, and a chest CT scan and that I will wait for my turn since the ER was full (took me 3 hours). After the interview with the ER physician, I was led to a seat one meter apart from others. Elderly patients with cough all quizzically looked at me in my white doctor’s gown wondering if I was sick. I changed to the gown, mask, and gloves I was provided with when the nurse escorted me to cubicle one. First came the CBC. Next were Rt-PCR swabs of the nose and nasopharynx and of the throat. Finally after the staff sterilized the CT scan room, my scan was completed in a few minutes. The chest scan showed ground glass appearance consistent with Bilateral Basal Pneumonia. I was told that the PCR results would be ready within 7 days. I was advised urgently by my classmate, a pulmonary specialist from another hospital, for admission for oxygen inhalation and treatment. She went out of her way to look for a pulmonologist but none was available due to quarantine. She looked for an Infectious Disease Specialist who was now in isolation. I requested her that since I was not in respiratory distress and because of my weakened state, I was worried about getting a hospital acquired infection and that I be committed to strict home isolation with treatment prescribed by her. She reluctantly acceded with the admonition that I proceed immediately back to hospital if respiratory difficulties occur. I started the Oseltamivir (Tamiflu) at 2x a day for 5 days and Azithromycin once daily for 7 days plus a mucolytic N- Acetylcysteine 600 mg 2x a day. There was another dimension beyond my physical stress. I was in MENTAL stress, the pervasive fear of not surviving this that engulfed me. Knowing my close colleagues died from COVID-19 pneumonia after a short battle in ICU with intubation, I realized THIS certainty of death and THAT uncertainty of recovery. I asked for a lifeline from my UP Med ‘76 classmates. (The lifeline in the family was unconditionally given though from a distance). My pulmonologist classmate closely monitored my condition daily. Some offered their listening ears to my echoing worries. Most prayed to God with their unconditional love for me to recover. Another classmate had extraordinary pranic sessions for my healing, my relaxation and my energy. I reflected on my dad’s advice that in a righteous fight (like against this pneumonia), “you use all means and all ways to win.” I started deep breathing into the nose and slowly out through the mouth knowing full well that the nitric oxide I absorbed through the roof of the nose dilated my coronaries for better heart function and my pulmonary arteries for better oxygen exchange. The deep breathing provided nitric oxide to the bronchus and bronchioles to dilate them for more airflow. Moreover, I was taking in lots of calamansi juices (or lemon or oranges) for its citrulline which has been researched to prolong the effects of nitric oxide.13 These breathing sessions were the MOST IMPORTANT activities if I were to survive and were continuous morning, noon and evening. Fortunately, I was isolated in 3rd floor Music Room with access to the roof deck garden and fresh air from Laguna de Bay (about 1.5 km from the house in Taguig) and of course, LPs of the Beatles, Aiza and Sharon, and Mozart. I did chest thumping or percussion as far as I could reach my back to loosen the phlegm in my lungs. This self ‘physical therapy’ was 3x a day. I started to spit scanty whitish, thick phlegm. I made sure that Oseltamivir (Tamiflu) was taken mornings and evenings and the Azithromycin was taken at lunch so there were no drug interactions. The 2 Colostrum tabs were swallowed on waking up. The probiotics were taken after breakfast and after dinner. The Zinc was taken after lunch. Soft stools were present but that was my GIT reacting to the medications. Adding to the controversy was the new regimen US President Donald Trump was trumpeting on Fox News and CNN. A hospitalist physician treating COVID-19 pneumonias in San Francisco was giving us the new protocols for Chloroquine and Azithromycin, with promising results. He was the classmate of my daughter in UP Med. I went to pharmacies in Taguig and Greenhills for Chloroquine. It was not available. My pulmonologist classmate was firm; “No!” when I suggested the shift. “You will need confinement and an ECG because these combination drugs prolong QTc on electrocardiogram.” This meant Chloroquine and Azithromycin combination may initially precipitate bradycardia (lower heart rate) then ventricular tachycardia (heightened heart rate), and finally, cardiac arrest for senior patients (68 years old) like me with a history of hypertension. This discussion stopped all controversies in treatment. Moreover, I was getting better. My temperature decreased to 37.8 °C. The muscle pain diminished. The severe point tenderness over the lower ribs persisted. I was deep-breathing which I could not hold for more than 10 seconds. That was not normal! I listened to my lungs for the CRACKLING sounds of pneumonia with my stethoscope. The maze of gurgling and churning sounds from the stomach and intestines seemed to mask the sounds I was listening for. Or was I in denial? I decided to go back to ER on the 3rd day for a chest X ray. The objective was to see if my pneumonia was progressing. The chest X ray still showed basal pneumonia. I had mixed feelings-- good that pneumonia did not progress to middle lung fields and --- bad that pneumonia was festering. I completed the 5-day regimen of Oseltamivir (Tamiflu) and was continuing the 8th day of Azithromycin and N-Acetylcysteine when my COVID test finally arrived through email-- I was COVID positive #1906 . By this time, I was recovering physically and mentally. I had no fever (37.2 °C average), no cough, no sore throat. Breathing was full. I had my appetite back. My outlook was as OPTIMISTIC as the blooming flowers I nurtured during this trial. This timing was fortunate because even with confirmed COVID-19 positive, I knew I had beaten COVID-19 pneumonia at home. Isolation was completed 2 weeks from my recovery which necessitated another COVID test and rapid test April 10, 2020. This test was still positive. A third PCR done on April 20 was negative for SARSCoV-2. The new DOH protocol was to isolate up to May 5 which I have followed. I am practicing social distancing and wearing a mask.

Starch quality is one of the most important agronomic traits in rice (Oryza sativa L). In this study, we identified single nucleotide polymorphisms (SNPs) in the Waxy and Alk genes of eight rice varieties and their associations with starch physicochemical properties.vi.e.vamylose content (AC) and gelatinization temperature (GT). Seven Sri Lankan rice varieties, Pachchaperumal, Herathbanda, At 354, Bg 352, Balasuriya, H 6 and Bw 295-5 were detected as high amylose varieties while Nipponbare exhibited low amylose content. In silico analysis of the Waxy gene revealed that all tested Sri Lankan varieties possessed ‘G’ (Wxa allele) instead of ‘T’ in the first intron which could explain varieties with high and intermediate amylose content. All Sri Lankan varieties had ‘A’ instead of ‘C’ in exon 6 of the Waxy gene and this fact was tally with the varieties showing high amylose content. Therefore, possessing the Wxa allele in the first intron and ‘A’ in exon 6 could be used as a molecular marker for the selection of high amylose varieties as validated using several Sri Lankan varieties. All Sri Lankan varieties except, Bw 295-5 exhibited the intermediate type of GT which could not be explained using the so far reported allelic differences in the Alk gene. However, Bw 295-5 which is a low GT variety had two nucleotide polymorphisms in the last exon of the Alk gene, i.e. ‘G’ and ‘TT’ that represent low GT class. Therefore, it can be concluded that sequence variations of Waxy and Alk genes reported in this study are useful in breeding local rice varieties with preferential amylose content and GT class.Key word Alk gene, amylose content, single nucleotide polymorphism, Waxy gene.INTRODUCTIONRice (Oryza sativa L.) is one of the leading food crops of the world. More than half of the world’s population relies on rice as the major daily source of calories and protein (Sartaj and Suraweera, 2005). After grain yield, quality is the most important aspect of rice breeding. Grain size and shape largely determine the market acceptability of rice, while cooking quality is influenced by the properties of starch. In rice grains starch is the major component that primarily controls rice quality. Starch consists of two forms of glucose polymers, relatively unbranched amylose and a highly branched amylopectin. Starch-synthesizing genes may contribute to variation in starch physicochemical properties because they affect the amount and structure of amylose and amylopectin in rice grain (Kharabian-Masouleh et al., 2012). Amylose content (AC), gelatinization temperature (GT) and gel consistency (GC) is the three most important determinants of eating and cooking quality. Amylose content is the ratio of amylose amount present in endosperm to total starch content. Rice varieties are grouped based on their amylose content into waxy (0-2%), very low (3-9%), low (10-19%), intermediate (20-25%), and high (> 25%) (Kongseree and Juliano, 1972). The most widely used method for amylose determination is a colorimetric assay where iodine binds with amylose to produce a blue-purple color, which is measured spectrophotometrically at a single wavelength (620nm). Low amylose content is usually associated with tender, cohesive and glossy cooked rice; while, high amylose content is associated with firm, fluffy and separate grains of cooked rice. The Waxy (Wx) gene, which encodes granule-bound starch synthase I (GBSSI), is the major gene controlling AC in rice (Nakamura, 2002). The Waxy gene is located on chromosome six and various single nucleotide polymorphisms (SNPs) of Wx were found, including a ‘G’ to ‘T’ SNP of the first intron, ‘A’ to ‘C’ SNP of the sixth exon and ‘C’ to ‘T’ SNP of the tenth exon (Larkin and Park, 2003). The ‘AGGTATA’ sequence at the 5’splice-junction coincides with the presence of the Wxa allele, while the ‘AGTTATA’ sequence coincides with the presence of the Wxb allele. Therefore, all intermediate and high amylose cultivars had ‘G’ nucleotide while low amylose cultivars had ‘T’ nucleotide at the putative leader intron 5′ splice site. The cytosine and thymidine (CT) dinucleotide repeats in the 5’- untranslated region (UTR) of the Waxy gene were reported to be a factor associated with AC. However, the relationship between these polymorphisms and amylose contents is not clear. Amylopectin chain length distribution plays a very important role to determine GT in cooked rice. The time required for cooking is determined by the gelatinization temperature of starch. It is important because it affects the texture of cooked rice and it is related to the cooking time of rice. The gelatinization temperature is estimated by the alkali digestibility test. It is measured by the alkali spreading value (ASV). The degree of spreading value of individual milled rice kernels in a weak alkali solution (1.7% KOH) is very closely correlated with gelatinized temperature. According to the ASV, rice varieties may be classified as low (55 to 69°C), intermediate (70 to 74°C) and high (> 74°C) GT classes. In a breeding program ASV is extensively used to estimate the gelatinization temperature. The synthesis of amylopectin is more complex than that of amylose. Polymorphisms in the starch synthase IIa (SSIIa) gene which is recognized as the Alk gene are responsible for the differences in GT in rice (Umemoto and Aoki, 2005; Waters et al., 2006). Two single nucleotide polymorphisms (SNPs) in the last exon of the Alk gene are responsible for the differences in GT in rice. The biochemical analysis clearly showed that the function of the amino acids caused by these two SNPs is essential for SSIIa enzyme activity (Nakamura et al., 2005) and those are ‘G’/‘A’ SNP at 4424 bp position and ‘GC’/‘TT’ SNPs at 4533/4534 bp position with reference to Nipponbare rice genomic sequence. Based on the SNPs, Low SSIIa enzyme activity results in S-type amylopectin, which is enriched in short chains whereas high SSIIa enzyme activity produces L-type amylopectin (Umemoto et al., 2004). Therefore, the combination of ‘G’ at SNP3 and ‘GC’ at SNP4 is required to produce L-type rice starch and this has a higher GT relative to S-type starch. GC is a standard assay that is used in rice improvement programs to determine the texture of softness and firmness in high amylose rice cultivars. Intermediate and low amylose rice usually has soft gel consistency. Sequence variation in exon 10 of the Waxy gene associates with GC (Tran et al., 2011).OBJECTIVES The objectives of this study were to detect polymorphisms in major starch synthesizing genes among several rice cultivars as models and to determine the relationship between their SNP variations and starch physicochemical properties. Also, we analyzed major starch synthesizing gene sequences of several Sri Lankan rice varieties in silico aiming at utilizing this information in rice breeding programs.MATERIALS AND METHODSPlant materials: Seeds of eight Oryza sativa L. accessions were obtained from the Rice Research and Development Institute (RRDI), Bathalagoda, Sri Lanka and Gene Bank of Plant Genetic Resource Center (PGRC), Gannoruwa.Characterization of grain physical parameters: Grain length and width were determined using a vernier caliper. Ten grains from each sample were collected randomly and measured to obtain the average length and width of the milled rice. The average length and width were recorded as their length and width. Based on the length and width of the grains, the milled rice grains were classified into four classes (table 1) according to the method accepted by RRDI Bathalagoda, Sri Lanka.According to the scale L/S – Long Slender, L/M – Long Medium, I/B – Intermediate Bold and S/R –Short RoundAnalysis of amylose content: Initially, rice samples were dehusked and polished prior to milling. Ten whole – milled rice kernels of eight rice samples were ground separately by using mortar and pestle. Amylose content per 100 mg was determined by measuring the blue value of rice varieties as described by Juliano (1971). About 100mg rice sample was shifted into a 100 mL volumetric flask and 1mL of 95% ethanol was added. Then 9mL of 1N NaOH was added and the content was boiled for 20min. at boiling temperature to gelatinize the starch. After cooling the content, the volume was made up to 100mL and 5mL of starch solution was pipetted out into a 100mL volumetric flask. The blue color was developed by adding 1mL of 1N acetic acid and 2 mL of iodine solution (0.2g iodine and 2.0g potassium iodine in 10 mL aqueous solution). Then volume was made up to 100mL with distilled water and the solution was kept for 20min. after shaking. Finally, the absorbance of the solution was measured at 620nm using Spectrophotometer T80 (PG Instruments Limited) as described by Juliano (1971). The standard curve was prepared using 40mg of potato-amylose to calculate the amylose content of rice varieties through absorbance values. Forty mg of potato amylose was put into a 100 mL of volumetric flask and 1ml of 95% ethanol and 9mL of NaOH were added and content was heated for 20min at boiling temperature. After cooling the content volume of the solution was made up to 100mL using distilled water. Then 1mL, 2mL, 3mL, 4mL and 5mL of amylose solution were pipetted out into 100mL flasks. Then 0.2mL, 0.4mL, 0.6mL, 0.8mL and 1mL of 1N acetic acid were added to the flasks respectively. Finally, 2mL of iodine solution was added to each flask and volume was made up to 100mL with distilled water. Solutions were stood up for 20min. after shaking and absorbance values were measured at 620nm. Measured absorbance values were plotted at 620nm against the concentration of anhydrous amylose (mg).Analysis of gelatinization temperature: GT was indirectly measured on rice by the alkali spreading value. Husked and polished seeds per accession were used for the analysis. Selected duplicate sets of six milled grains without cracks of each sample were put into Petri dishes. About 10mL of 1.7% KOH was added and grains were spread in the petri dish to provide enough space. The constant temperature at 30°C was maintained to ensure better reproducibility. After 23hrs, the degree of disintegration was quantified by a standard protocol with a numerical scale of 1–7 (table 2) as reported by Cruz and Khush (2000). As reported by Juliano (2003), GT of rice was determined using the alkaline spreading scale, where 1.0-2.5: High (74-80 °C), 2.6-3.4: High-intermediate (70-74 °C), 3.5-5.4: Intermediate (70-74 °C) and 5.5-7.0 Low: (55-70 °C).Bioinformatics and statistical analysis: The available literature was used to identify the most likely candidate genes associated with rice starch quality and their SNPs of each gene (Hirose et al., 2006; Waters and Henry, 2007; Tran et al., 2011). In all the tested varieties except Bg 352 and At 354, the DNA sequence of each gene was retrieved from the Rice SNP Seek database (http://snp-seek.irri.org/). The gene sequences of At 354 and Bg 352 were obtained from the National Research Council 16-016 project, Wayamba University of Sri Lanka. Multiple sequence alignment was conducted for the DNA sequence using Clustal Omegavsoftware (https://www.ebi.ac.uk/Tools/msa/clustalo/). Starch physiochemical data obtained were subjected to a one-way analysis of variance (ANOVA) followed by Duncan’s New Multiple Range Test (DNMRT) to determine the statistical differences among varieties at the significance level of p ≤ 0.05. Statistical analysis was done using SAS version 9.1 (SAS, 2004).ESULTS AND DISCUSSION: Physical properties of rice grains: Physical properties such as length, width, size, shape and pericarp color of rice grains obtained from eight different rice varieties are given in table 3. Classification of rice grains was carried out, according to their sizes and shapes based on Juliano (1985). The size of the rice grains was determined as per grain length while grain shape was determined by means of length and width ratio of the rice kernel. In the local market, rice is classified as Samba (short grain), Nadu (intermediate grain) and Kora (long/medium) based on the size of the grain (Pathiraje et al., 2010). Lengths of rice kernels were varied from 5.58 to 6.725 mm for all varieties. The highest grain length and width were given by At 354 and Pachchaperumal respectively. The varieties, Bw 295-5 and H 6 showed a length: width ratio over 3 which is considered as slender in grain shape. Bw 295-5, H 6, At 354, Bg 352 and Nipponbare possessed white pericarp and others possessed red pericarp.Relationship between amylose content and SNPs variation of waxy loci in selected varieties: Amylose content was measured in seven Sri Lankan rice varieties and one exotic rice variety. Amylose content of the evaluated varieties varied significantly with p ≤ 0.05 with the lowest of 15.11% and highest of 28.63% which were found in Nipponbare and Bw 295-5, respectively (table 4). The majority of the evaluated varieties fell into the high AC category (between 25-28%). Only Nipponbare could be clearly categorized under the low amylose group (table 4). The amylose content of Bg 352, Pachchaperumal and Herathbanda have already been determined by early studies of Rebeira et al. (2014) and Fernando et al. (2015). Most of the data obtained in the present experiment has agreed with the results of previous studies. Major genes such as Waxy and their functional SNPs have a major influence on amylose in rice (Nakamura et al., 2005). Accordingly, single nucleotide polymorphism, ‘G’/‘T’, at the 5’ leader intron splice site of the GBSSI has explained the variation in amylose content of varieties. Accordingly, high and intermediate amylose varieties have ‘AGGTATA’ while low amylose varieties have the sequence ‘AGTTATA’, which might lead to a decrease in the splicing efficiency. Therefore, the GBSSI activity of Nipponbare might be considerably weak and resulted in starch with low amylose content. Hence, producing ‘G’/‘T’ polymorphism clearly differentiates low amylose rice varieties, as reported by Nakamura et al. (2005). In GBSSI, Larkin and Park (2003) identified an ‘A’/‘C’ polymorphism in exon 6 and a ‘C’/‘T’ polymorphism in exon 10 which resulted in non- synonymous amino acid change. Chen et al. (2008) reported that the non-synonymous ‘A’/‘C’ SNP at exon 6 had the highest possible impact on GBSSI. Accordingly, the ‘A’/‘C’ polymorphism in exon 6 causes a tyrosine/serine amino acid substitution while the ‘C’/‘T’ polymorphism in exon 10 causes a serine/proline amino acid substitution. In view of this information, there is a relationship between the polymorphism detected by in silico analysis and amylose content obtained from our experiment. Out of the eight tested rice varieties, only one variety, Nipponbare was categorized as low amylose variety (10-19%) and it exhibited ‘T’ nucleotide at the intron splice site (table 4; figure 1). Varieties such as Pachchaperumal, Balasuriya, Bw 295-5, H 6, Herathbanda, At 354 and Bg 352 which contained high amylose (> 25%), had ‘G’ and ‘A’ nucleotides at intron splice site and exon 6 respectively (table 4; figure 1). The predominant allelic pattern of intron splice site and exon 6 are different in varieties containing intermediate amylose content (20-25%) which showed ‘G’ and ‘C’ nucleotides respectively. Of these selected rice varieties, none of the intermediate type amylose variety was found.Relationship between gel consistency and SNPs variation in Waxy loci: In this study, GC data of Herathbanda, Hondarawalu, Kuruluthuda, Pachchaperumal and Bg 352 were obtained from Fernando et al. (2015). The results of Tran et al. (2011) showed that the exon 10 ‘C’/‘T’ SNP of Wx has mainly affected GC. Accordingly, rice with a ‘C’ at exon 10 had soft and viscous gels once cooked. However, a sample with a ‘T’ had short and firm gels. In this study, Herathbanda, Hondarawalu, Kuruluthuda and Pachchaperumal had ‘C’ nucleotide and Bg 352 had ‘T’ nucleotide in exon 10 (table 5; figure 2). However, ‘C’/‘T’ substitution analysis could not be used to explain the GC of tested varieties.Relationship between gelatinized temperature and SNPs variation of Alk loci in selected rice varieties: Although there were differences in the scores, the degree of disintegration of all samples was saturated at 23 hrs. Most of the selected rice varieties showed the intermediate disintegration score. Varieties, Pachchaperumal, Balasuriya, H 6, Herathbanda, At 354 and Bg 352 were categorized into intermediate GT class (70–74°C) as indicated by an alkali spreading (AS) value of 5 (table 6; figure 3). Nipponbare and Bw 295-5 showed the highest disintegration score indicating the dispersion of all grains. Hence these varieties were categorized into low GT class (55-69°C) as indicated by an AS value of 6 (table 6; figure 3). However, high GT class rice varieties (> 74°C) were not found in the tested samples. Chromosomal mutation within the Alk gene has led to a number of single nucleotide polymorphisms (SNPs). Umemoto et al. (2004) identified four SNPs in Alk gene. Thus, SNP3 and SNP4 may be important genetic polymorphisms that are associated with GT class. According to the SNP3 and SNP4, eight rice varieties could be classified into either high GT or low GT types. If there is ‘A’ instead of ‘G’ at 4424 bp position of Alk gene with reference to Nipponbare rice genomic sequence, it codes methionine instead of valine amino acid residue in SSIIa, whilst two adjacent SNPs at bases 4533 and 4534 code for either leucine (‘GC’) or phenylalanine (‘TT’). Rice varieties with high GT starch had a combination of valine and leucine at these residues. Rice varieties with low GT starch had a combination of either methionine and leucine or valine and phenylalanine at these same residues. Nipponbare carried the ‘A’ and ‘GC’ nucleotides, while Bw 295-5 carried the ‘G’ and ‘TT’ nucleotides. Hence these varieties were classified into low GT class. Varieties such as Pachchaperumal, Balasuriya, H 6, Herathbanda, At 354 and Bg 352 carried ‘G’ and ‘GC’ nucleotides and these varieties were classified into high GT rice varieties. However, intermediate GT status could not be determined by SNP3 and SNP4 mutation of Alk gene (table 6; figure 4).In silico analysis of the polymorphisms in GBSSI gene and Alk genes of rice varieties retrieved from Rice-SNP-database: In this study, GBSSI gene and Alk gene were compared with the sequences retrieved from the Rice-SNP-Seek database to validate the SNPs further. As previously reported by Ayres et al. (1997), all low amylose varieties had the sequence ‘AGTTATA’ in exon 1. In agreement with preliminary work done by Larkin and Park (2003), all of the intermediate amylose varieties have the allelic pattern of GCC. All of the high amylose varieties have either the GAC or GAT allele of GBSSI. Among 42 rice accessions with the Sri Lankan pedigree, four allelic patterns were found; TAC, GCC, GAC and GAT (table 7). In this allelic pattern, the first letter corresponds to the ‘G’/‘T’ polymorphism in 5’ leader intron splice-junction, the second letter corresponds to the ‘A’/‘C’ polymorphism in exon 6 and the third letter corresponds to the ‘C’/‘T’ polymorphism in exon10 of Waxy gene. Analysis of the ‘G’/‘T’ polymorphism in the Wx locus showed that 41 rice cultivars shared the same ‘AGGTATA’ sequence at the 5’ leader intron splice-junction. But only 1 rice cultivar, Puttu nellu was found with ‘T’ nucleotide in intron1/exon1 junction site, which could be categorized as a low amylose variety (table 7). As discussed above, varieties with an intermediate level of apparent amylose could be reliably distinguished from those with higher apparent amylose based on a SNP in exon 6. Hence, only three rice varieties Nalumoolai Karuppan, Pannithi and Godawel with ‘C’ nucleotide in exon 6 exhibited the possibility of containing intermediate amylose content (table 7). High activity of GBSSI produces high amylose content leading to a non-waxy, non-sticky or non-glutinous phenotype. Therefore, according to the in silico genotypic results, rest of the 38 rice varieties may produce high amylose content in the endosperm (table 7). Proving this phenomenon. Abeysekera et al. (2017) has reported that usually, most of Sri Lankan rice varieties contain high amylose content. Targeted sequence analysis of exon 8 of the Alk gene in 42 different rice cultivars were found with three SNP polymorphisms that resulted in a changed amino acid sequence and, of these three SNPs, two SNPs were reported to be correlated with possible GT differences. Accordingly, Puttu nellu and 3210 rice varieties carried the ‘G’ and ‘TT’ nucleotides in SNP3 and SNP4 respectively (table 7). Hence these varieties can be classified into low GT class and except these two; other rice varieties carried the ‘G’ and ‘GC’ nucleotides in SNP3 and SNP4 respectively. Therefore, those varieties can possibly be classified into high GT rice varieties (table 7). However, further experiments are necessary to check the phenotypic variations for grain amylose content and GT class of in silico analyzed rice varieties. CONCLUSION Present results revealed the relationship between SNPs variation at Waxy loci and the amylose content of selected rice varieties. Accordingly, Pachchaperumal, At 354, Bg 352, Herathbanda, H 6, Balasuriya and Bw 295-5 with high amylose content had ‘G’ instead of ‘T’ in the first intron exhibiting the presence of Wxa allele with reference to Nipponbare which had low amylose content. Also all tested varieties had ‘A’ in exon 6 of the Waxy gene. Thus present findings i.e. presence of Wxa allele and SNP ‘A’ in exon 6 could be used as a potential molecular marker for the selection of high amylose varieties. In addition, Bw 295-5 which is a low GT variety, had two SNPs variations in the last exon of the Alk gene i.e. ‘G’ and ‘TT’ which is likely to be used to represent low GT class. Accordingly, sequence variations identified in Waxy and Alk genes could be utilized in the future rice breeding programs for the development of varieties with preferential amylose content and GT class.ACKNOWLEDGMENTSDirector and staff of the Gene Bank, Plant Genetic Resources Center, Gannoruwa are acknowledged for giving rice accessions.CONFLICT OF INTERESTAuthors have no conflict of interest.REFERENCESAbeysekera, W., G. Premakumara, A. Bentota and D. S. Abeysiriwardena, 2017. Grain amylose content and its stability over seasons in a selected set of rice varieties grown in Sri Lanka. Journal of agricultural sciences Sri Lanka, 12(1): 43-50.Ayres, N., A. McClung, P. Larkin, H. Bligh, C. Jones and W. Park, 1997. Microsatellites and a single-nucleotide polymorphism differentiate apparentamylose classes in an extended pedigree of us rice germ plasm. Theoretical applied genetics, 94(6-7): 773-781.Chen, M.-H., C. Bergman, S. Pinson and R. Fjellstrom, 2008. Waxy gene haplotypes: Associations with apparent amylose content and the effect by the environment in an international rice germplasm collection. Journal of cereal science, 47(3): 536-545.Cruz, N. D. and G. Khush, 2000. Rice grain quality evaluation procedures. Aromatic rices, 3: 15-28.Fernando, H., T. Kajenthini, S. Rebeira, T. Bamunuarachchige and H. Wickramasinghe, 2015. Validation of molecular markers for the analysis of genetic diversity of amylase content and gel consistency among representative rice varieties in sri lanka. Tropical agricultural research, 26(2): 317-328.Hirose, T., T. Ohdan, Y. Nakamura and T. Terao, 2006. Expression profiling of genes related to starch synthesis in rice leaf sheaths during the heading period. Physiologia plantarum, 128(3): 425-435.Juliano, B., 1971. A simplified assay for milled rice amylose. Journal of cereal science today, 16: 334-360.Juliano, B. O., 1985. Rice: Chemistry and technology. The american association of cereal chemists. Inc. 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ABSTRACT From the onset of World War II, America needed men of integrity, ability, and proven leadership to facilitate the wartime economy—the nation turned to CPAs for help. In 1941, the president of the American Institute of Accountants, N. Loyall McLaren, stepped away from his accounting firm and from his Institute presidency to serve in the U.S. Navy where he handled a variety of accounting-related duties. McLaren was a pioneer California CPA, serving as a partner in the largest firm in the Golden State, and had served as president of the California Society of CPAs. He later was a powerful business leader in the San Francisco area; his firm eventually merged with Haskins & Sells. This is the story of Commander (later Rear Admiral; also Army Brigadier General) McLaren and his service to the nation and the profession during World War II and thereafter.

This minicase is one of 10 in a series written to illustrate the business benefits companies realize through adopting sustainable business strategies. Method is a successful startup firm in San Francisco that has made sustainable design principles a guiding force its strategy. Unlike other firms, however, Method sells its products on performance, price, and aesthetics; health and environment are simply built into the design from the outset. Backed by venture capital and successful selling through Target nationwide, Method offers a model of what may ultimately become standard practice and strategy.

Abstract Much research on disruptive innovations has focused on firms that disrupt existing industries. Yet, regulators and lawmakers are instrumental in containing or enabling market disruption, in ways that are less understood. This article examines taxi industries in New York City, Chicago and San Francisco between 2010 and 2014 to better understand the role of regulators and lawmakers in enabling Uber to disrupt these industries. Relying on 142 interviews, ethnographic observations and primary source documents, I show that regulators and lawmakers used two strategies in responding to Uber: blocking and incorporating. Blocking refers to measures that stop a firm from entering the industry. Incorporating refers to adding, subtracting or modifying regulations to align with an innovative firm’s practices. I identify three incorporating strategies: horizontal venue shifting, vertical venue shifting and reinterpreting existing regulations. Analyzing these strategies more clearly illuminates regulatory change mechanisms and lawmakers’ and regulators’ role in enabling disruptive innovations.

The Muwekma Ohlone Tribe has long been involved in the archaeology and stewardship of their ancestral homelands, both through their own cultural resource management (CRM) firm and though collaborations with academic and CRM archaeologists. In this article, we build on the past 40 years of archaeological collaborations in the southern San Francisco Bay region and offer examples of how archaeologists can support tribal heritage and environmental stewardship by using the traditional purview of material culture in combination with a broader array of evidence and concerns. As presented in our brief case studies, the Muwekma Ohlone Tribe and scholars are working together to reclaim tribal heritage and promote Native stewardship in a cultural landscape that has been marred by more than 250 years of dispossession. We examine this work in the context of the renaming of ancestral sites, the public interpretation of Native heritage associated with Mission Santa Clara de Asís, archival research into the history of Indigenous resistance, as well as collaborative efforts to awaken traditional ecological knowledge in service of the Tribe's stewardship and land management goals.

The rapid growth of tech company headquarters such as Apple, Facebook, and Google could potentially put new pressure on the housing market in adjacent residential neighborhoods, in the form of housing price appreciation and real estate speculation. This article examines the relationship between the big tech corporate campuses and Silicon Valley/San Francisco housing markets using the Zillow (ZTRAX) transaction and tax assessor data. The authors compare real estate activity adjacent to new company locations with activity in nearby areas, conducting a difference-in-differences analysis to estimate changes in housing prices and speculation. They find that housing prices increase overall by an additional 7.1% in the immediate vicinity of the tech campus 2 years after arrival, with wide variation across campuses. The authors also identify significant real estate speculation occurring prior to firms’ arrival. This suggests that cities should take a proactive role in mitigating tech firm impacts on vulnerable adjacent neighborhoods.

Research methodology This case is based on an in-depth interview with Sean Ansett on March 6, 2020 in San Francisco. For a good reference book on the interview method in social science, please see Seidman (2019). Ansett is an alumnus of the Lam Family College of Business at San Francisco State University. A follow-up interview was conducted on December 13, 2021, via Zoom. The case situations are factual, but the names of the luxury brand, the factory and the Tunisian social auditing firm were disguised. Selected video clips of the interviews are available upon request. Case overview/synopsis In 2010, Sean Ansett, a social auditor with more than 25 years of experience in promoting workers’ rights in the global supply chain, faced a momentous decision. He was hired by a luxury brand company to conduct a social audit of a Tunisian leather goods factory. During his visit to the factory, he observed the troubling signs of child labor and alarming health and safety concerns in the work environment. Should he report the factory’s situation to the local authority? What should he advise his client, the luxury brand company, to do? Ansett realized that this was not a cut-and-dried decision as reporting to the local authority may affect workers adversely if the factory was closed. This case highlights the ethical dilemmas of human rights in the global supply chain. It also raises critical questions for multinational firms regarding what constitutes an ethical brand and how to ensure effective code of conduct implementation. Complexity academic level This case can be used in undergraduate or graduate business courses or curated sessions and seminars related to corporate social responsibility, ethics and social auditing in supply chain management.

Founded in San Francisco in 2009, Square finished 2012 as the darling of Silicon Valley; flush with more than $340 million in funding, the firm had grown to several hundred employees in just three short years. It processed more than $10 billion annually in credit and debit card payments from small business owners that used Square’s smartphone-enabled card swipe device wherever cellular or wireless Internet service was available.However, Square’s success had attracted new entrants into the mobile payments processing space, both in the United States and abroad, threatening to derail the company’s remarkable trajectory. With its latest financing round valuing the company in excess of $3.4 billion, management and investors were considering which strategies would continue—even accelerate—the company’s growthSquare presents an opportunity for classes in strategy and technology management to contemplate the following: How can a startup disrupt an established set of incumbents without provoking a harsh competitive response? How can a growth company in a rapidly changing industry expand beyond the core competency that fueled its initial growth? Which growth platforms make the most sense for a company in a complicated ecosystem with many players offering divergent solutions?

W gospodarce globalnej opartej na wiedzy najszybciej rozwijające się startupy są jednocześnie najbardziej innowacyjne i zinternacjonalizowane. Autorzy stawiają hipotezę, iż koncentrowanie publicznego wsparcia na przedsiębiorstwach o wysokim potencjale wzrostu (startupach) jest elementem integrującym kluczowe polityki w obszarze badań, innowacji, przedsiębiorczości i internacjonalizacji. W celu weryfikacji hipotezy przeprowadzono analizę z trzech perspektyw badawczych: narodowych systemów innowacyjnych w krajach UE i organizacji pomostowych w Dolinie Krzemowej (Część I opublikowana w „Przeglądzie Organizacji” 2017, Nr 12, s. 5–14) oraz innowacyjnych startupów z Polski wchodzących na rynek USA poprzez Zatokę San Francisco. Grupa badawcza firm przebadana została w Polsce i w Dolinie Krzemowej, a głównym celem badania było określenie, w jaki sposób projektować systemy wsparcia internacjonalizacji, innowacyjności i przedsiębiorczości, tak aby w jak największym stopniu odpowiadały one na potrzeby startupów o największym potencjale wzrostu. W większości badanych przypadków firmy korzystały z zaawansowanego wsparcia internacjonalizacji (Polski Most Krzemowy, Go Global), co przyczyniło się do zwiększenia stopnia ich innowacyjności, w tym w szczególności usprawnień związanych z oferowanym produktem oraz modyfikacjami strategii marketingowej. Z perspektywy przedsiębiorstw polityka wsparcia startupów powinna integrować programy związane z internacjonalizacją oraz innowacyjnością. Wyniki badania stanowią rekomendację dla kreatorów polityki wsparcia scaleupów o większe zintegrowanie instrumentów aktywizowania internacjonalizacji z polityką wspierania innowacyjności, w tym wzmocnienia obecności rządowych Organizacji Pomostowych w światowych hubach technologicznych.

PurposeThe purpose of this paper is to describe how resilience unfolded in a project-based organization with the support of organizational values through changing leadership styles. The rapidly announced restrictions on businesses during the coronavirus disease 2019 (COVID-19) provided an opportunity to observe and study resilience unfold.Design/methodology/approachThe process-perspective case study approach of a structural and civil engineering design firm in San Francisco, California, USA, integrates interviews, observations, document analysis and information tracking via email and Microsoft Teams. The researchers adopted a leadership perspective, where the units of analysis are the internal management and the employees' behaviors.FindingsIn the case examined, the capability represented in the organizational values influenced the choice of situation-appropriate leadership styles to support employees. The values of relationship, passion and trust influenced the dominant choice of a transformational style, where stability and excellence facilitate a transactional style – all equally important for the balance and resilience of the project-based organization.Originality/valueThis study demonstrated that when organizational values support leaders in cultivating a learning environment, those values provide stability for leaders to promote resilience. To the best of the researchers' knowledge, no previous work described how situational-, transformational- and transactional-leadership styles evolve in response to a crisis and together facilitate organizational resilience.

From 1957 to 1973, Barcelona Zoo was transformed from a small-scale, antiquated establishment harboring very few animals, a place that was still in a poor condition following the Spanish Civil War, into a new, larger, modern, and internationally recognized institution that included up-to-date animal enclosures and that boasted one of the first dolphinariums in Europe, as well as a famous white gorilla as its icon. From its very beginning, this renovation involved an intense popularization campaign. In this paper, by describing the public discourse generated throughout this transformation and by analyzing the roles played by Antoni Jonch, the director of the zoo at that time; Josep Maria de Porcioles, the then-mayor of Barcelona; and General Francisco Franco, the Spanish dictator who was head of state during this period, I will highlight how the context of the dictatorship not only authorized the new Barcelona Zoo but is, in fact, crucial to its understanding. The narratives that surrounded the renovation of the zoo focused on its civic and educational purpose, with a firm emphasis on modernity. The zoo was a modern space for learning about animals in order to become better people. These narratives were not only suitable to the policies, ideas, and aims of Porcioles’ City Council and Franco’s dictatorial regime; in fact, they completely matched them. Both local and national governments benefited from the restructuring of Barcelona Zoo and from its public discourse as a tool for social control and an instrument for their own propaganda, legitimation, and perpetuation.

Despite an increasing number of studies focusing on leadership at Vietnamese small and medium sized enteprises (SMEs), there is a lack of empirical research on collective leadership and development of effective top management team (TMT). The purpose of this paper is to review the literature with a view of characteristics forming an effective TMT. Taking the team effectiveness theory, upper echelons theory and behavioral theory of the firm, theoretical characteristics of effective TMT was created, and then an empirical research was conducted on a sample of 141 SMEs. The common characteristics of an effective TMT are identified, including shared vision, optimal team size, role clarity, age diversity, functional background diversity, regular communication, solidarity and collective decision making. The study makes several contributions to the existing literature on effective TMT development at SMEs. 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On December 27, 1951, Prime Minister Yoshida Shigeru sent John F. Dulles a letter that explained “Counter Infiltration" against China. Yoshida thought the best way to wean Chinese from the Communist regime was by sending people into China through trade activities and encouraging an anticommunist movement in China. He believed that Japan could have a major role in such an operation. The purpose of this paper is to examine Yoshida's “Counter Infiltration" plan against China from the standpoint of intelligence. Yoshida, taking a special interest in intelligence, established intelligence organs such as the Public Security Intelligence Agency and the Cabinet Research Office (CRO) in quick succession soon after the San Francisco Peace Treaty went into effect in April 1952. Worried about indirect aggression from communist countries, Yoshida concentrated his efforts on developing an interior intelligence framework. At the same time, he tried to foster the growth of a Japanese intelligence organization that could gather information and perform covert operations in Mainland China.This study shows that Yoshida proactively tried to strengthen intelligence cooperation with governments of both Taiwan and the United States. Yoshida appointed Ogata Taketora Chief Cabinet Secretary and made him supervisor of Japanese intelligence organs. Ogata urged the Nationalist government on Taiwan to cooperate in establishing a Communist information exchange organ, and asked the U.S. Central Intelligence Agency (CIA) for assistance in creating a Japanese CIA. On the other hand, Yoshida let retired lieutenant General Tatsumi Eiichi recruit ex-military personnel for service in the CRO. With the assistance of Tatsumi, the CRO started actual intelligence activity against China after January 1953. The CRO interrogated repatriates from China, and proposed a joint operation with the CIA to send Japanese agents into Macao. Thus Yoshida tried to establish a Japanese intelligence system and backed U.S. strategy against China in the intelligence field.Yoshida's idea, however, was frustrated by rapid changes at home and abroad. After the Peace Treaty came into force, Yoshida couldn't maintain a firm hold on power. Not only the opposition parties but also the media criticized Ogata's plan to launch a Japanese CIA. In the end, Ogata had no choice but to downscale his ambitious plans, and eliminate overseas covert operations. Moreover, Yoshida's confrontational approach against the Chinese government was criticized for being behind the times after the Indochina armistice in 1954. In the last days of his ministry, Yoshida encouraged both Britain and U.S. to set up a “high command" on China in Singapore. His aim was to use overseas Chinese based in Southeast Asia to infiltrate Mainland China, but his idea wasn't put into practice because he was unable to gain the support of either Britain and the United States or even his own entourage.

The segment of my work containing song lyrics was inspired by a question: Was the Beat Movement an isolated period of thought, tied to one time and location that generated the Beat Generation writers' unique ideas and inquisitive minds? Certainly, the ideas of the Beats were not confined to one city, or even one country: we can trace their travels to San Francisco, New York, Palo Alto, the Baja Peninsuala, Guatemala, Tangiers, London, Paris, Rome, and places in between. The Beats, like other anti-establishment cultural movements, were tied to an historical moment, in that case post-war America, but their ideas resonate backwards and forwards, from political and artistic revolutions in Europe to the counter-culture movement of the 1960s and 1970s. Their work gave rise to debates in our Beat Generation class as to whether or not our contemporary society could give birth to such a movement. As a group, our class concluded that no, our world today is too digitized and too image-focused to facilitate a counter-culture movement. There is too much media and too much emphasis on consumerism to allow for a mainstream Beat movement. I saw it otherwise, for I am a firm believer that modern Beat ideas exist all around us. It's easy to find remnants of the Beats in Indie music, and I created a playlist entitled counter-culture baby, inspired by a lyric in Flipturn’s song “Hippies.” Over the course of a few days, I listened to all 158 songs, a total of 9 hours, 38 minutes of music,and I concluded that they lyrics that resonated in my head afterwards expressed similar sentiments to Beat artists. I ultimately chose to include five sets of lyrics in my beat journal, although the album covers of many others are arranged on the preceding and following pages. Moving from top to bottom of the page, there are excerpts from “Vanilla” by Flipturn, “Malibu 1992” by COIN, “Astrovan” by Mt. Joy, “Holy Moly, Rock n’ Rolly, Guacamole” by Lazy Ghost, and “Chicago” by Flipturn. Though many songs in the playlist demonstrated counter-culture and anti-establishment messages, and I thought it was important to choose five songs that resonated with Beat poems. The singers rage against modern America, they expression the fear of a mundane lifestyle, the use of religious figures in an unconventional, pro-freedom manner, and even the creation of new words. Whether these artists know the works of Ginsberg, Kerouac, Burroughs or not, they employ similar techniques and express common ideas throughout their art. As for the segment of cut-ups, one of the goals of the creation of “Être Beat” was to live my life as though I myself was a Beat. I looked at the stars, I debated philosophy late at night, I wrote a manifesto. I sought perspective-altering experiences by going skydiving, learning the bass guitar, and by attending jazz clubs. And in response, I created these works by consciously employing Beat techniques. For instance, I drew inspiration from Gysin and Burroughs in creating a “cut-up” of Verlaine’s poems. I chose Verlaine because his work was often read and discussed in the Beat Hotel. Thus, in as random of a manner as possible, I cut up some of his poems, including but not limited to Chanson à manger, L’angoisse, and Dans les limbes. The result was astounding for me. With scissors and glue, I was able to create ideas I wouldn’t have been able to create with only a pen and paper. Of course, the words are Verlaine’s -- but the manner in which they are arranged give them a completely new, abstract, and symbolic meaning.

During the harvest of 2020 and 2021, sweet cherry (Prunus avium) fruit showed a firm rot with irregular pale to dark brown lesions on the fruit surface, with green to light brown fungal growth resembling Alternaria-like infection (Simmons, 2007). Diseased cherries (n= 80 fruit) were collected at harvest in mature (over 10-year-old) commercial orchards of cherry tree varieties Lapins, Regina, Santina, Skeena, and Sweetheart planted in four localities of the regions O´Higgins (33°59´ S, 70°42´W; San Francisco de Mostazal and Graneros) and Maule (35°00’S, 71°23´W; Curicó and Sagrada Familia), Central Chile. The incidence of black rot was 1.9 and 3.2% in O´Higgins and Maule region, respectively, and it was increased to up to 5% during cold storage. The fruit collected previously, were transported to the lab, and surface disinfected in 75% ethanol for 15 s, and rinsed in sterile water. Internal pieces from the junction of diseased and healthy tissues of fruits were placed on potato dextrose agar (PDA, 2%) for 7 days at 20°C. Forty-two isolates of Alternaria-like (Simmons, 2007) were recovered consistently from pure cultures taking hyphal tips from 7 days old cultures. On PDA, 28 isolates (group A) were characterized by cottony, white-gray to green colonies and conidial chains (4 to 10 conidia) with secondary chains (1 to 5 conidia) branching on the conidiophore. Conidia were ovate to obclavate (mean 22.8 ± 5.1 x 8.8 ± 1.5 μm; n=40) with 3 to 7 transepta and 1 longisepta. The remaining 14 isolates (group B) were characterized by cottony, olive-green to olive-brown colonies following a ring pattern of growth and white margins, with conidial chains (4 to 14 conidia) and uncommon secondary chains (1 to 4 conidia). Conidia were obpyriform to ovate, light brown to brown with a cylindrical short beak at the tip (mean 24.7 ± 5.9 × 11.2 ± 1.3 μm; n=40) with 2 to 4 transepta, and 0 to 2 longisepta. Two representative isolates of group A (Sant-02-2020 and Bing-03-2020) and group B (Sant-26-2021 and Skeen-43-2021) were amplified for the Alternaria major allergen (Alt a1), plasma membrane ATPase (ATP), and calmodulin (Cal) loci following the protocols described by Hong et al. (2005) and Lawrence et al. (2013). A MegaBlast search of sequences of group A (GenBank nos. OR267293- OR267294, OR258001- OR258002, and OR267297- OR267298, for Alt a1, ATP, and Cal, respectively) showed 100% similarity to strains UCD10529 and UCD10539 of A. alternata, and group B (GenBank nos. OR267295- OR267296, OR258003- OR258004, and OR258005- OR258006, for Alt a1, ATP, and Cal, respectively) showed 100% similarity to strains EGS 34-015 and A30 of A. tenuissima. Combined phylogenetic analysis using MEGA X clustered isolates Sant-02-2020 and Bing-03-2020, and Sant-26-2021 and Skeen-43-2021 with ex-type of A. alternata and A. tenuissima, respectively. Pathogenicity tests were conducted using isolates of A. alternata (Sant-02-2020; Bing-03-2020) and A. tenuissima (Sant-26-2021; Skeen-43-2021). Detached ripe cherry fruit var. Sweetheart (n=40 fruits/isolate) and Regina (n=40 fruits/isolate) were surfaces disinfested (75% ethanol, 30 s), wounded in the middle with a sterile needle (2 mm in depth), and inoculated with 20 μL of conidial suspension (106 conidia/mL). An equal number of healthy cherries (n=40 fruits) treated with sterile water were used as controls. The experiment was repeated once. All inoculated fruit incubated for 7 days at 22°C, developed between 13 ± 2.7 to 23 ± 2.5 mm and 14.1 ± 1.1 to 19 ± 3.6 mm in lesion diameter for A. alternata and A. tenuissima isolates, respectively. Koch´s postulates were fulfilled by 100% reisolation of the causal pathogen from inoculated fruit, and molecular identification of A. alternata and A. tenuissima isolates. Previously, A. alternata has been described as causing rots on cherries in Chile (Acuña 2010), and China (Zhao and Liu, 2012; Ahmad et al., 2020). To our knowledge, this is the first occurrence of cherry black rot caused by A. alternata and A. tenuissima in Central Chile. Epidemiological studies are necessary to develop integrated management of cherry black rot in Central Chile.

I first came across etoy in Linz, Austria in 1995. They turned up at Ars Electronica with their shaved heads, in their matching orange bomber jackets. They were not invited. The next year they would not have to crash the party. In 1996 they were awarded Arts Electronica’s prestigious Golden Nica for web art, and were on their way to fame and bitterness – the just rewards for their art of self-regard. As founding member Agent.ZAI says: “All of us were extremely greedy – for excitement, for drugs, for success.” (Wishart & Boschler: 16) The etoy story starts on the fringes of the squatters’ movement in Zurich. Disenchanted with the hard left rhetorics that permeate the movement in the 1980s, a small group look for another way of existing within a commodified world, without the fantasy of an ‘outside’ from which to critique it. What Antonio Negri and friends call the ‘real subsumption’ of life under the rule of commodification is something etoy grasps intuitively. The group would draw on a number of sources: David Bowie, the Sex Pistols, the Manchester rave scene, European Amiga art, rumors of the historic avant gardes from Dada to Fluxus. They came together in 1994, at a meeting in the Swiss resort town of Weggis on Lake Lucerne. While the staging of the founding meeting looks like a rerun of the origins of the Situationist International, the wording of the invitation might suggest the founding of a pop music boy band: “fun, money and the new world?” One of the – many – stories about the origins of the name Dada has it being chosen at random from a bilingual dictionary. The name etoy, in an update on that procedure, was spat out by a computer program designed to make four letter words at random. Ironically, both Dada and etoy, so casually chosen, would inspire furious struggles over the ownership of these chancey 4-bit words. The group decided to make money by servicing the growing rave scene. Being based in Vienna and Zurich, the group needed a way to communicate, and chose to use the internet. This was a far from obvious thing to do in 1994. Connections were slow and unreliable. Sometimes it was easier to tape a hard drive full of clubland graphics to the underside of a seat on the express train from Zurich to Vienna and simply email instructions to meet the train and retrieve it. The web was a primitive instrument in 1995 when etoy built its first website. They launched it with a party called etoy.FASTLANE, an optimistic title when the web was anything but. Coco, a transsexual model and tabloid sensation, sang a Japanese song while suspended in the air. She brought media interest, and was anointed etoy’s lifestyle angel. As Wishart and Bochsler write, “it was as if the Seven Dwarfs had discovered their Snow White.” (Wishart & Boschler: 33) The launch didn’t lead to much in the way of a music deal or television exposure. The old media were not so keen to validate the etoy dream of lifting themselves into fame and fortune by their bootstraps. And so etoy decided to be stars of the new media. The slogan was suitably revised: “etoy: the pop star is the pilot is the coder is the designer is the architect is the manager is the system is etoy.” (Wishart & Boschler: 34) The etoy boys were more than net.artists, they were artists of the brand. The brand was achieving a new prominence in the mid-90s. (Klein: 35) This was a time when capitalism was hollowing itself out in the overdeveloped world, shedding parts of its manufacturing base. Control of the circuits of commodification would rest less on the ownership of the means of production and more on maintaining a monopoly on the flows of information. The leading edge of the ruling class was becoming self-consciously vectoral. It controlled the flow of information about what to produce – the details of design, the underlying patents. It controlled the flows of information about what is produced – the brands and logos, the slogans and images. The capitalist class is supplanted by a vectoral class, controlling the commodity circuit through the vectors of information. (Wark) The genius of etoy was to grasp the aesthetic dimension of this new stage of commodification. The etoy boys styled themselves not so much as a parody of corporate branding and management groupthink, but as logical extension of it. They adopted matching uniforms and called themselves agents. In the dada-punk-hiphop tradition, they launched themselves on the world as brand new, self-created, self-named subjects: Agents Zai, Brainhard, Gramazio, Kubli, Esposto, Udatny and Goldstein. The etoy.com website was registered in 1995 with Network Solutions for a $100 fee. The homepage for this etoy.TANKSYSTEM was designed like a flow chart. As Gramazio says: “We wanted to create an environment with surreal content, to build a parallel world and put the content of this world into tanks.” (Wishart & Boschler: 51) One tank was a cybermotel, with Coco the first guest. Another tank showed you your IP number, with a big-brother eye looking on. A supermarket tank offered sunglasses and laughing gas for sale, but which may or may not be delivered. The underground tank included hardcore photos of a sensationalist kind. A picture of the Federal Building in Oklamoma City after the bombing was captioned in deadpan post-situ style “such work needs a lot of training.” (Wishart & Boschler: 52) The etoy agents were by now thoroughly invested in the etoy brand and the constellation of images they had built around it, on their website. Their slogan became “etoy: leaving reality behind.” (Wishart & Boschler: 53) They were not the first artists fascinated by commodification. It was Warhol who said “good art is good business.”(Warhol ) But etoy reversed the equation: good business is good art. And good business, in this vectoral age, is in its most desirable form an essentially conceptual matter of creating a brand at the center of a constellation of signifiers. Late in 1995, etoy held another group meeting, at the Zurich youth center Dynamo. The problem was that while they had build a hardcore website, nobody was visiting it. Agents Gooldstein and Udatny thought that there might be a way of using the new search engines to steer visitors to the site. Zai and Brainhard helped secure a place at the Vienna Academy of Applied Arts where Udatny could use the computer lab to implement this idea. Udatny’s first step was to create a program that would go out and gather email addresses from the web. These addresses would form the lists for the early examples of art-spam that etoy would perpetrate. Udatny’s second idea was a bit more interesting. He worked out how to get the etoy.TANKSYSTEM page listed in search engines. Most search engines ranked pages by the frequency of the search term in the pages it had indexed, so etoy.TANKSYSTEM would contain pages of selected keywords. Porn sites were also discovering this method of creating free publicity. The difference was that etoy chose a very carefully curated list of 350 search terms, including: art, bondage, cyberspace, Doom, Elvis, Fidel, genx, heroin, internet, jungle and Kant. Users of search engines who searched for these terms would find dummy pages listed prominently in their search results that directed them, unsuspectingly, to etoy.com. They called this project Digital Hijack. To give the project a slightly political aura, the pages the user was directed to contained an appeal for the release of convicted hacker Kevin Mitnick. This was the project that won them a Golden Nica statuette at Ars Electronica in 1996, which Gramazio allegedly lost the same night playing roulette. It would also, briefly, require that they explain themselves to the police. Digital Hijack also led to the first splits in the group, under the intense pressure of organizing it on a notionally collective basis, but with the zealous Agent Zai acting as de facto leader. When Udatny was expelled, Zai and Brainhard even repossessed his Toshiba laptop, bought with etoy funds. As Udatny recalls, “It was the lowest point in my life ever. There was nothing left; I could not rely on etoy any more. I did not even have clothes, apart from the etoy uniform.” (Wishart & Boschler: 104) Here the etoy story repeats a common theme from the history of the avant gardes as forms of collective subjectivity. After Digital Hijack, etoy went into a bit of a slump. It’s something of a problem for a group so dependent on recognition from the other of the media, that without a buzz around them, etoy would tend to collapse in on itself like a fading supernova. Zai spend the early part of 1997 working up a series of management documents, in which he appeared as the group’s managing director. Zai employed the current management theory rhetoric of employee ‘empowerment’ while centralizing control. Like any other corporate-Trotskyite, his line was that “We have to get used to reworking the company structure constantly.” (Wishart & Boschler: 132) The plan was for each member of etoy to register the etoy trademark in a different territory, linking identity to information via ownership. As Zai wrote “If another company uses our name in a grand way, I’ll probably shoot myself. And that would not be cool.” (Wishart & Boschler:: 132) As it turned out, another company was interested – the company that would become eToys.com. Zai received an email offering “a reasonable sum” for the etoy.com domain name. Zai was not amused. “Damned Americans, they think they can take our hunting grounds for a handful of glass pearls….”. (Wishart & Boschler: 133) On an invitation from Suzy Meszoly of C3, the etoy boys traveled to Budapest to work on “protected by etoy”, a work exploring internet security. They spent most of their time – and C3’s grant money – producing a glossy corporate brochure. The folder sported a blurb from Bjork: “etoy: immature priests from another world” – which was of course completely fabricated. When Artothek, the official art collection of the Austrian Chancellor, approached etoy wanting to buy work, the group had to confront the problem of how to actually turn their brand into a product. The idea was always that the brand was the product, but this doesn’t quite resolve the question of how to produce the kind of unique artifacts that the art world requires. Certainly the old Conceptual Art strategy of selling ‘documentation’ would not do. The solution was as brilliant as it was simple – to sell etoy shares. The ‘works’ would be ‘share certificates’ – unique objects, whose only value, on the face of it, would be that they referred back to the value of the brand. The inspiration, according to Wishart & Boschsler, was David Bowie, ‘the man who sold the world’, who had announced the first rock and roll bond on the London financial markets, backed by future earnings of his back catalogue and publishing rights. Gramazio would end up presenting Chancellor Viktor Klima with the first ‘shares’ at a press conference. “It was a great start for the project”, he said, “A real hack.” (Wishart & Boschler: 142) For this vectoral age, etoy would create the perfect vectoral art. Zai and Brainhard took off next for Pasadena, where they got the idea of reverse-engineering the online etoy.TANKSYSTEM by building an actual tank in an orange shipping container, which would become etoy.TANK 17. This premiered at the San Francisco gallery Blasthaus in June 1998. Instant stars in the small world of San Francisco art, the group began once again to disintegrate. Brainhard and Esposito resigned. Back in Europe in late 1998, Zai was preparing to graduate from the Vienna Academy of Applied Arts. His final project would recapitulate the life and death of etoy. It would exist from here on only as an online archive, a digital mausoleum. As Kubli says “there was no possibility to earn our living with etoy.” (Wishart & Boschler: 192) Zai emailed eToys.com and asked them if them if they would like to place a banner ad on etoy.com, to redirect any errant web traffic. Lawyers for eToys.com offered etoy $30,000 for the etoy.com domain name, which the remaining members of etoy – Zai, Gramazio, Kubli – refused. The offer went up to $100,000, which they also refused. Through their lawyer Peter Wild they demanded $750,000. In September 1999, while etoy were making a business presentation as their contribution to Ars Electronica, eToys.com lodged a complaint against etoy in the Los Angeles Superior Court. The company hired Bruce Wessel, of the heavyweight LA law firm Irell & Manella, who specialized in trademark, copyright and other intellectual property litigation. The complaint Wessel drafted alleged that etoy had infringed and diluted the eToys trademark, were practicing unfair competition and had committed “intentional interference with prospective economic damage.” (Wishart & Boschler: 199) Wessel demanded an injunction that would oblige etoy to cease using its trademark and take down its etoy.com website. The complaint also sought to prevent etoy from selling shares, and demanded punitive damages. Displaying the aggressive lawyering for which he was so handsomely paid, Wessel invoked the California Unfair Competition Act, which was meant to protect citizens from fraudulent business scams. Meant as a piece of consumer protection legislation, its sweeping scope made it available for inventive suits such as Wessel’s against etoy. Wessel was able to use pretty much everything from the archive etoy built against it. As Wishart and Bochsler write, “The court papers were like a delicately curated catalogue of its practices.” (Wishart & Boschler: 199) And indeed, legal documents in copyright and trademark cases may be the most perfect literature of the vectoral age. The Unfair Competition claim was probably aimed at getting the suit heard in a Californian rather than a Federal court in which intellectual property issues were less frequently litigated. The central aim of the eToys suit was the trademark infringement, but on that head their claims were not all that strong. According to the 1946 Lanham Act, similar trademarks do not infringe upon each other if there they are for different kinds of business or in different geographical areas. The Act also says that the right to own a trademark depends on its use. So while etoy had not registered their trademark and eToys had, etoy were actually up and running before eToys, and could base their trademark claim on this fact. The eToys case rested on a somewhat selective reading of the facts. Wessel claimed that etoy was not using its trademark in the US when eToys was registered in 1997. Wessel did not dispute the fact that etoy existed in Europe prior to that time. He asserted that owning the etoy.com domain name was not sufficient to establish a right to the trademark. If the intention of the suit was to bully etoy into giving in, it had quite the opposite effect. It pissed them off. “They felt again like the teenage punks they had once been”, as Wishart & Bochsler put it. Their art imploded in on itself for lack of attention, but called upon by another, it flourished. Wessel and eToys.com unintentionally triggered a dialectic that worked in quite the opposite way to what they intended. The more pressure they put on etoy, the more valued – and valuable – they felt etoy to be. Conceptual business, like conceptual art, is about nothing but the management of signs within the constraints of given institutional forms of market. That this conflict was about nothing made it a conflict about everything. It was a perfectly vectoral struggle. Zai and Gramazio flew to the US to fire up enthusiasm for their cause. They asked Wolfgang Staehle of The Thing to register the domain toywar.com, as a space for anti-eToys activities at some remove from etoy.com, and as a safe haven should eToys prevail with their injunction in having etoy.com taken down. The etoy defense was handled by Marcia Ballard in New York and Robert Freimuth in Los Angeles. In their defense, they argued that etoy had existed since 1994, had registered its globally accessible domain in 1995, and won an international art prize in 1996. To counter a claim by eToys that they had a prior trademark claim because they had bought a trademark from another company that went back to 1990, Ballard and Freimuth argued that this particular trademark only applied to the importation of toys from the previous owner’s New York base and thus had no relevance. They capped their argument by charging that eToys had not shown that its customers were really confused by the existence of etoy. With Christmas looming, eToys wanted a quick settlement, so they offered Zurich-based etoy lawyer Peter Wild $160,000 in shares and cash for the etoy domain. Kubli was prepared to negotiate, but Zai and Gramazio wanted to gamble – and raise the stakes. As Zai recalls: “We did not want to be just the victims; that would have been cheap. We wanted to be giants too.” (Wishart & Boschler: 207) They refused the offer. The case was heard in November 1999 before Judge Rafeedie in the Federal Court. Freimuth, for etoy, argued that federal Court was the right place for what was essentially a trademark matter. Robert Kleiger, for eToys, countered that it should stay where it was because of the claims under the California Unfair Competition act. Judge Rafeedie took little time in agreeing with the eToys lawyer. Wessel’s strategy paid off and eToys won the first skirmish. The first round of a quite different kind of conflict opened when etoy sent out their first ‘toywar’ mass mailing, drawing the attention of the net.art, activism and theory crowd to these events. This drew a report from Felix Stalder in Telepolis: “Fences are going up everywhere, molding what once seemed infinite space into an overcrowded and tightly controlled strip mall.” (Stalder ) The positive feedback from the net only emboldened etoy. For the Los Angeles court, lawyers for etoy filed papers arguing that the sale of ‘shares’ in etoy was not really a stock offering. “The etoy.com website is not about commerce per se, it is about artist and social protest”, they argued. (Wishart & Boschler: 209) They were obliged, in other words, to assert a difference that the art itself had intended to blur in order to escape eToy’s claims under the Unfair Competition Act. Moreover, etoy argued that there was no evidence of a victim. Nobody was claiming to have been fooled by etoy into buying something under false pretences. Ironically enough, art would turn out in hindsight to be a more straightforward transaction here, involving less simulation or dissimulation, than investing in a dot.com. Perhaps we have reached the age when art makes more, not less, claim than business to the rhetorical figure of ‘reality’. Having defended what appeared to be the vulnerable point under the Unfair Competition law, etoy went on the attack. It was the failure of eToys to do a proper search for other trademarks that created the problem in the first place. Meanwhile, in Federal Court, lawyers for etoy launched a counter-suit that reversed the claims against them made by eToys on the trademark question. While the suits and counter suits flew, eToys.com upped their offer to settle to a package of cash and shares worth $400,000. This rather puzzled the etoy lawyers. Those choosing to sue don’t usually try at the same time to settle. Lawyer Peter Wild advised his clients to take the money, but the parallel tactics of eToys.com only encouraged them to dig in their heels. “We felt that this was a tremendous final project for etoy”, says Gramazio. As Zai says, “eToys was our ideal enemy – we were its worst enemy.” (Wishart & Boschler: 210) Zai reported the offer to the net in another mass mail. Most people advised them to take the money, including Doug Rushkoff and Heath Bunting. Paul Garrin counseled fighting on. The etoy agents offered to settle for $750,000. The case came to court in late November 1999 before Judge Shook. The Judge accepted the plausibility of the eToys version of the facts on the trademark issue, which included the purchase of a registered trademark from another company that went back to 1990. He issued an injunction on their behalf, and added in his statement that he was worried about “the great danger of children being exposed to profane and hardcore pornographic issues on the computer.” (Wishart & Boschler: 222) The injunction was all eToys needed to get Network Solutions to shut down the etoy.com domain. Zai sent out a press release in early December, which percolated through Slashdot, rhizome, nettime (Staehle) and many other networks, and catalyzed the net community into action. A debate of sorts started on investor websites such as fool.com. The eToys stock price started to slide, and etoy ‘warriors’ felt free to take the credit for it. The story made the New York Times on 9th December, Washington Post on the 10th, Wired News on the 11th. Network Solutions finally removed the etoy.com domain on the 10th December. Zai responded with a press release: “this is robbery of digital territory, American imperialism, corporate destruction and bulldozing in the way of the 19th century.” (Wishart & Boschler: 237) RTMark set up a campaign fund for toywar, managed by Survival Research Laboratories’ Mark Pauline. The RTMark press release promised a “new internet ‘game’ designed to destroy eToys.com.” (Wishart & Boschler: 239) The RTMark press release grabbed the attention of the Associated Press newswire. The eToys.com share price actually rose on December 13th. Goldman Sachs’ e-commerce analyst Anthony Noto argued that the previous declines in the Etoys share price made it a good buy. Goldman Sachs was the lead underwriter of the eToys IPO. Noto’s writings may have been nothing more than the usual ‘IPOetry’ of the time, but the crash of the internet bubble was some months away yet. The RTMark campaign was called ‘The Twelve Days of Christmas’. It used the Floodnet technique that Ricardo Dominguez used in support of the Zapatistas. As Dominguez said, “this hysterical power-play perfectly demonstrates the intensions of the new net elite; to turn the World Wide Web into their own private home-shopping network.” (Wishart & Boschler: 242) The Floodnet attack may have slowed the eToys.com server down a bit, but it was robust and didn’t crash. Ironically, it ran on open source software. Dominguez claims that the ‘Twelve Days’ campaign, which relied on individuals manually launching Floodnet from their own computers, was not designed to destroy the eToys site, but to make a protest felt. “We had a single-bullet script that could have taken down eToys – a tactical nuke, if you will. But we felt this script did not represent the presence of a global group of people gathered to bear witness to a wrong.” (Wishart & Boschler: 245) While the eToys engineers did what they could to keep the site going, eToys also approached universities and businesses whose systems were being used to host Floodnet attacks. The Thing, which hosted Dominguez’s eToys Floodnet site was taken offline by The Thing’s ISP, Verio. After taking down the Floodnet scripts, The Thing was back up, restoring service to the 200 odd websites that The Thing hosted besides the offending Floodnet site. About 200 people gathered on December 20th at a demonstration against eToys outside the Museum of Modern Art. Among the crowd were Santas bearing signs that said ‘Coal for eToys’. The rally, inside the Museum, was led by the Reverend Billy of the Church of Stop Shopping: “We are drowning in a sea of identical details”, he said. (Wishart & Boschler: 249-250) Meanwhile etoy worked on the Toywar Platform, an online agitpop theater spectacle, in which participants could act as soldiers in the toywar. This would take some time to complete – ironically the dispute threatened to end before this last etoy artwork was ready, giving etoy further incentives to keep the dispute alive. The etoy agents had a new lawyer, Chris Truax, who was attracted to the case by the publicity it was generating. Through Truax, etoy offered to sell the etoy domain and trademark for $3.7 million. This may sound like an insane sum, but to put it in perspective, the business.com site changed hands for $7.5 million around this time. On December 29th, Wessel signaled that eToys was prepared to compromise. The problem was, the Toywar Platform was not quite ready, so etoy did what it could to drag out the negotiations. The site went live just before the scheduled court hearings, January 10th 2000. “TOYWAR.com is a place where all servers and all involved people melt and build a living system. In our eyes it is the best way to express and document what’s going on at the moment: people start to about new ways to fight for their ideas, their lifestyle, contemporary culture and power relations.” (Wishart & Boschler: 263) Meanwhile, in a California courtroom, Truax demanded that Network Solutions restore the etoy domain, that eToys pay the etoy legal expenses, and that the case be dropped without prejudice. No settlement was reached. Negotiations dragged on for another two weeks, with the etoy agents’ attention somewhat divided between two horizons – art and law. The dispute was settled on 25th January. Both parties dismissed their complaints without prejudice. The eToys company would pay the etoy artists $40,000 for legal costs, and contact Network Solutions to reinstate the etoy domain. “It was a pleasure doing business with one of the biggest e-commerce giants in the world” ran the etoy press release. (Wishart & Boschler: 265) That would make a charming end to the story. But what goes around comes around. Brainhard, still pissed off with Zai after leaving the group in San Francisco, filed for the etoy trademark in Austria. After that the internal etoy wranglings just gets boring. But it was fun while it lasted. What etoy grasped intuitively was the nexus between the internet as a cultural space and the transformation of the commodity economy in a yet-more abstract direction – its becoming-vectoral. They zeroed in on the heart of the new era of conceptual business – the brand. As Wittgenstein says of language, what gives words meaning is other words, so too for brands. What gives brands meaning is other brands. There is a syntax for brands as there is for words. What etoy discovered is how to insert a new brand into that syntax. The place of eToys as a brand depended on their business competition with other brands – with Toys ‘R’ Us, for example. For etoy, the syntax they discovered for relating their brand to another one was a legal opposition. What made etoy interesting was their lack of moral posturing. Their abandonment of leftist rhetorics opened them up to exploring the territory where media and business meet, but it also made them vulnerable to being consumed by the very dialectic that created the possibility of staging etoy in the first place. By abandoning obsolete political strategies, they discovered a media tactic, which collapsed for want of a new strategy, for the new vectoral terrain on which we find ourselves. Works Cited Negri, Antonio. Time for Revolution. Continuum, London, 2003. Warhol, Andy. From A to B and Back Again. Picador, New York, 1984. Stalder, Felix. ‘Fences in Cyberspace: Recent events in the battle over domain names’. 19 Jun 2003. <http://felix.openflows.org/html/fences.php>. Wark, McKenzie. ‘A Hacker Manifesto [version 4.0]’ 19 Jun 2003. http://subsol.c3.hu/subsol_2/contributors0/warktext.html. Klein, Naomi. No Logo. Harper Collins, London, 2000. Wishart, Adam & Regula Bochsler. Leaving Reality Behind: etoy vs eToys.com & Other Battles to Control Cyberspace Ecco Books, 2003. Staehle, Wolfgang. ‘<nettime> etoy.com shut down by US court.’ 19 Jun 2003. http://amsterdam.nettime.org/Lists-Archives/nettime-l-9912/msg00005.html Links http://amsterdam.nettime.org/Lists-Archives/nettime-l-9912/msg00005.htm http://felix.openflows.org/html/fences.html http://subsol.c3.hu/subsol_2/contributors0/warktext.html Citation reference for this article Substitute your date of access for Dn Month Year etc... MLA Style Wark, McKenzie. "Toywars" M/C: A Journal of Media and Culture< http://www.media-culture.org.au/0306/02-toywars.php>. APA Style Wark, M. (2003, Jun 19). Toywars. M/C: A Journal of Media and Culture, 6,< http://www.media-culture.org.au/0306/02-toywars.php>

IntroductionDreams feature frequently in poetry as framing devices for lyrical and narrative content. By contrast, Thom Gunn’s poem “The Annihilation of Nothing” (1958) theorises how dream, through its relationship to the states of sleeping and waking up, serves the creative process of practising poets. To this extent, Gunn is a challenging fellow traveller in the dream-poetry tradition. “The Annihilation of Nothing” is usually categorised by critics as a philosophical poem informed by Existentialist notions. This article supplements such a reading by showing how the poem’s Existentialism is related specifically to the creative process of writing poetry. Theory about the practice of writing poetry is potentially to be found as much in poetry itself as in commentary about poetry. Ultimately, Gunn’s poem sutures its philosophical concerns to the understanding of the poet’s creative process first theorised by John Keats as Negative Capability. Gunn’s poem suggests an abstract and structural approach to the use of dream to write poetry, informed by Keatsian negativity, which exploits that creatively fertile moment when sleep transforms into the waking state. Sigmund Freud’s assertion that sleep is the essential condition of dream supports the idea that, for Gunn, sleep in itself may have the power of dream for the writing of poetry. “The Annihilation of Nothing” offers practical guidance, informed by theory and philosophy, to poets keen to insert themselves into the dream-poetry tradition. Bolstering the reading of “The Annihilation of Nothing”, my article also considers an instance of Gunn’s non-fiction writing on poetry and dreams, in which he links “a series of anxiety dreams” to the writing of his poem “Jack Straw’s Castle” (1976). Dream Poetry and Thom Gunn The literature on the relationship of poetry and dreaming is massive, multi-faceted, and deeply impacted by the movement of history. A.C. Spearing’s Medieval Dream-Poetry reminds us that the literary combination of poetry and dreaming is many centuries old (Spearing passim). Since the late nineteenth century, the theories of Sigmund Freud and Carl Jung have invited new ways of activating the interpretation and creation of dream poetry (Shafton passim). A more recent contribution to the field of dream-poetry studies is Night Errands: How Poets Use Dreams, which foregrounds the voices of contemporary American poets reflecting on matters of poetry and dreaming through the personal essay form (Townley passim). In his article “Dream Poetry as Dream Work”, Richard A. Russo observes that “poets use dreams in many different ways” (13). My article works from the premise that dream-poetry can be identified and discussed even when a poet does not explicitly identify as a dream poet. The Anglo-American poet Thom Gunn (1929-2004) is not usually thought of as a dream poet; still, in an interview conducted in 1977, he states that “I think everything significant that happens to me—whether it’s an event, an idea or a dream—gets into my poetry eventually” (Scobie 13; my emphasis). Nor, so far as I am aware, has Gunn’s 1958 poem “The Annihilation of Nothing” ever been interpreted as a dream poem (Gunn, “Annihilation” 4). Framing Gunn’s poetry as dream poetry is therefore an unusual move in the appreciation of his work, which by that very token seeds fresh opportunities to contribute to the ongoing scholarly and practice-based conversations about dream poetry in general. As I will show in what follows, Gunn’s method of using dreams is very different from that of most other poets. Rather than fixating on, and elaborating, the details of this or that particular dream, Gunn works at a more general level, to explore how dreams relate to the poetic creative process. For this article, I am particularly interested in exploring how the powerfully activated philosophical elements of “The Annihilation of Nothing” intersect with an engagement with the craft of poetry making as this is influenced, not only by dream itself, but by the sleeping, dreaming and waking routine of Gunn as a practising poet. Additionally, I will be arguing that—against the grain of most dream poetry—Gunn tends to work with dream as a structural principle of poetic creation. This is evident not only in “The Annihilation of Nothing” but in Gunn’s non-fiction writing about the 1976 poem “Jack Straw’s Castle” (Gunn, “Jack” 48-56). This is not, however, to privilege the non-fiction voice of the poet over the voice within their poetry, in matters of theory. As much as theory about the practice of writing poetry is to be found in commentary about poetry, it is also to be found, I suggest, in poetry itself. Ultimately, Gunn contributes as much to the tradition of the poetic strategy of Negative Capability, as first theorised by the poet John Keats (“Negative Capability”), as to the tradition of dream poetry. “The Annihilation of Nothing”, Existentialism and the Craft of Poetry Writing “The Annihilation of Nothing” is a relatively short poem, and my reading of it for this article necessarily assumes close working knowledge of its nuances and complexities (if not its paradoxes) in the reader. For these reasons, I am including the text of the poem here, in full: Nothing remained: Nothing, the wanton nameThat nightly I rehearsed till led awayTo a dark sleep, or sleep that held one dream.In this a huge contagious absence lay,More space than space, over the cloud and slime,Defined but by the encroachments of its sway.Stripped to indifference at the turns of time,Whose end I knew, I woke without desire,And welcomed zero as a paradigm.But now it breaks—images burst with fireInto the quiet sphere where I have bided,Showing the landscape holding yet entire:The power that I envisaged, that presidedUltimate in its abstract devastations,Is merely change, the atoms it dividedComplete, in ignorance, new combinations.Only an infinite finitude I seeIn those peculiar lovely variations.It is despair that nothing cannot beFlares in the mind and leaves a smoky markOf dread. Look upward. Neither firm nor free,Purposeless matter hovers in the dark. (Gunn, “Annihilation” 4) “The Annihilation of Nothing” has been categorised by literary critics as one of Gunn’s philosophical poems and it is generally interpreted using an Existentialist lens. B.J.C. Hinton, for example, states that the poem “is based on an existentialist problem and adopts its terminology” (131). Additionally, “the poem is based on the paradox that nothingness, itself often a subject of terror, is seen first as comforting and then as illusory. This fusing of philosophical inquiry and genuine passion is typical of Gunn at his best, and gives the poem great force” (Hinton 143-144). Curiously, I have not been able to locate any reading of Gunn’s poem that unpacks what is arguably the poem’s most obvious detail: that is, the narrator falls asleep, experiences “a dark sleep, or sleep that held one dream”, then wakes up. Hinton notes that the poem’s “very title is based on terms common in [Jean-Paul] Sartre’s L’Etre et le neant” (143) but he overlooks the fact that the poem’s key term, “nothing”, is part of the narrator’s method of falling asleep: “Nothing, the wanton name / That nightly I rehearsed till led away / To a dark sleep.” When the word (“wanton name”) has served its purpose and the narrator has fallen asleep, precisely “Nothing remained”; the word has been replaced by the state it describes. Such a pointed differentiation of the word and the state invites the reader to grapple with that most intriguing of concepts: nothingness as a something that is nothing. The last line of the first stanza deepens the poetic meaning of nothingness in Gunn’s hands. “To a dark sleep, or sleep that held one dream” suggests an equivalence between “sleep” and “dream”. The comma before the “or” implies that a “sleep that held one dream” is just another way of saying “a dark sleep,” while the notion of “one dream” strengthens this implication—one sleep and one dream coincide with each other in their matching singularity. Dream, Gunn seems to be suggesting, is what “darkens” sleep; all the same, sleep always already retains strong cultural associations with darkness, which suggests that the poet is actually more concerned to hollow out the concept of dream, as a marker of nothingness. Dream, in this reading, thus exists in an ambiguous overlap with the notion of sleep itself; within this overlap, furthermore, dream’s nothingness consists in its absorption into sleep. In other words, the Existentialist philosophy of the poem is activated as a dialogue between the concepts of a dream that is nothing—one reduced, as it were, to the status of mere (dream-less) sleep—and the freshly waking state of the narrator, which identifies the period when Sartrean being rushes in to replace dream-sleep nothingness. Being and nothingness are overlaid onto, and to an extent replaced by, the twin states of waking from sleep and of a dream that, far from being any dream in particular, is characterised only as “a dark sleep”. When the philosophical or Existentialist framework for the reading of “The Annihilation of Nothing” is nudged to one side, though not abandoned, and the dream and waking-from-sleep elements of the poem are given their appropriate weight in the interpretation of the poem, an affiliation with the tradition of dream poetry emerges. Certainly, Gunn’s poem exhibits features linked to Sartre’s philosophical preoccupations, but its Existentialist engagement, I suggest, is narrowed to a concern with the borderline between sleep and the waking state as the condition of a certain mode of poetic creation. In this lies the connection to the tradition of dream poetry, and the distinctiveness of Gunn’s intervention into that tradition. To this extent, “The Annihilation of Nothing” straddles philosophy and the craft of poetry writing. The discussion of dream in an abstract or philosophical sense is precisely what makes Gunn’s poem a practical resource for other poets, with their own dreams to transform into poetry. Poetic creation, for Gunn, relates to the activation of those energies present at the borderline of sleep and being awake. These energies, in turn, relate to the theory of Negative Capability. Negative Capability in “The Annihilation of Nothing” To continue to unpack Gunn’s lesson in poetry-making, I underscore how odd it is, in the first place, that Hinton links Gunn’s poem to a work of prose philosophy by Jean-Paul Sartre rather than to a more poetically cognate antecedent. A more appropriate companion text to Gunn’s poem than Sartre’s Existentialist masterpiece, I suggest, is the poetic strategy known as Negative Capability. The Glossary of Poetic Terms managed by the Poetry Foundation discusses Negative Capability in terms of “the artist’s access to truth without the pressure and framework of logic or science” and defines it, in part, as “the [poet’s] power to bury self-consciousness [and] dwell in a state of openness to all experience” (“Negative Capability”). The poet John Keats was, of course, the first to articulate the theory of Negative Capability (“Negative Capability”). What evidence is there that Negative Capability, rather than Existentialism, is a more relevant accompaniment to Gunn’s poem? Stanzas two and three describe the nothingness of a dream that is only “a dark sleep” through reference to “a huge contagious absence” and to the comment “And welcomed zero as a paradigm.” Notice what is happening here. The absence is deemed “contagious”, and a “paradigm” is usually defined as a typical example or pattern of something. What appears to be going on here is an attempt to show how creation is triggered by concepts aligned with negativity, such as absence and zero. Such concepts further align, like unconscious sleep itself, with Keats’s “[buried] self-consciousness”. In mirror image, the same pattern shows up later on in the poem. Whereas the first three stanzas of “The Annihilation of Nothing” detail the descent into sleep, dream and nothingness, the stanzas after the fourth stanza are all about the creation that occurs upon waking, at the end of dream, with the advent of what might be called (creative) somethingness. Crucially, this period of creation possesses an element that mirrors the “contagious absence” and “zero as a paradigm” gestures from earlier in the poem. Previously, nothingness was expressed as a prompt to creation (“contagious” and “a paradigm”); here, in a 180 degree switch, somethingness is only indirectly expressed by reference to its polar opposite. The key line is “It is despair that nothing cannot be.” Creation is shadowed by a sort of negative energy; it comes about only through the annihilation of nothing, or “in [self-?] ignorance”: “The power that I envisaged, that presided / Ultimate in its abstract devastations, / Is merely change, the atoms it divided / Complete, in ignorance, new combinations.” The negative with a positive charge has been replaced by the positive with a negative charge, twin movements which reassert Keats’s theory of poetic creation. Nothingness prompts the creation of somethingness while somethingness always retains its debt to nothingness. Negative Capability thus tarries between the lines of “The Annihilation of Nothing.” Creation simmers away even in the midst of nothingness and absence (in the early stages of the poem), without the support of self-consciousness. Reversing and reinforcing this, the activism of poetic creation (which is conventionally based in self-consciousness) is strangely inverted in the final sections of the poem, where the somethingness of poetic creation is figured merely as a by-product of the “despair that nothing cannot be.” In fine, Gunn’s poem transfigures Existentialism in such a way that it produces a new version of Keats’s theory of Negative Capability. And Gunn’s intervention is precisely a theoretical intervention, to the extent that it produces transferable knowledge, about the production of poetry, related to how the writing of poetry may be structured in relationship to the division—at heart Existentialist—between sleeping (configured in Gunn’s poem as a new form of dreaming) and creatively waking up. There is an interesting crossover here between Gunn’s poem and Freud’s idea from The Interpretation of Dreams that “at bottom dreams are nothing other than a particular form of thinking, made possible by the conditions of the state of sleep” (Freud 510, fn. 1; my emphasis second). As part of his contribution to a theory of the creative practice of poetry writing, Gunn draws out something not always given its due weight in Freudian theory: that is, the crucial if not essential connection between sleep and dream. It is this connection, I suggest, that Gunn sees as vital to the practice of writing poetry in the tradition of dream poetry. The moment of waking from sleep is when (dream-like) Negative Capability may be most fruitfully activated. Indeed, Gunn comes close to suggesting, in “The Annihilation of Nothing”, that whether one dreams or not is inconsequential, so long as one has—quite mysteriously—“a dark sleep.” The sleep state itself—necessarily accompanied by the waking from sleep—may possess the creative power of dream when it comes to the writing of poetry. “Jack Straw’s Castle” and Dream-Poetry Structure Further theoretical knowledge may be harvested from another moment in Gunn’s discourse, in which he responds to an interviewer’s question about the process of writing “Jack Straw’s Castle”. As suggested above, I do not see a distinction, and certainly not a hierarchy, between theory about poetry writing to be found within poetry itself and theory about poetry writing constructed within a non-fictional context, as in the interview containing this exchange: WS [W.I. Scobie]: Why are there so many references to rooms in your poetry—not only rooms, but dungeons, cellars, cells—confining spaces?…TG [Thom Gunn]: While I was writing Jack Straw’s Castle I was moving from a house I’d lived in for ten years into this place [Gunn’s house in San Francisco], and I had a series of anxiety dreams. I had moved into the wrong house. I had moved in with the wrong people. Once to my horror I found I was sharing an apartment with [Richard] Nixon. Very often I would keep discovering new rooms in the house that I’d known nothing about. All this became very much the scheme for the nightmare world of Jack Straw’s Castle. (Scobie 11; my emphasis) Gunn’s theoretical attitude to dream in this non-fiction commentary bears similarities, I suggest, to his theorisation of dream in “The Annihilation of Nothing”. Rather than stressing the content of his “anxiety dreams” (for example, individual houses or Richard Nixon), Gunn characteristically emphasises the structural aspect of dream. His choice of words is significant: Gunn’s dreams become a “scheme” for the production of poetry. Granted, the content of “Jack Straw’s Castle” does include many references to rooms and the like, but Gunn segues, in this interview extract, from content to form. Notably, in this context, “Jack Straw’s Castle” is divided into eleven sections, as if the poet (in writing the poem) and the reader (in reading it) were moving from one section to the next the way one moves from one room to the next. (It is perhaps no coincidence that Gunn’s best-known poem is entitled “On the Move”.) The Existentialist and person-focused structural approach to the writing of poetry of “The Annihilation of Nothing” is replaced here by a more formal type of structure. Conclusion This article has attempted to locate Thom Gunn within the tradition of dream poetry even as he challenges the mainstream of that tradition. His challenge lies in how he constructs dream as a structural prompt to the writing of poetry, in a way that departs from the more familiar dream-poetry approach of elaborating the lyrical or narrative content of this or that particular dream. Gunn is both a practitioner of dream poetry and also, in the footsteps of John Keats and others, a theorist of the writing of poetry. Theory about the practice of writing poetry is potentially to be found as much in poetry itself as in commentary about poetry. The main focus of the article has been Gunn’s poem “The Annihilation of Nothing”. My intent has not been to show that the Existentialist reading of this poem is wrong, so much as to show that it must be supplemented by attention to the poem’s concern with dreaming, sleeping and waking and, by extension, to the specific Existentialism of the practising writer of poetry. Read this way, the poem presents itself as a text in the tradition of Negative Capability. References Freud, Sigmund. The Interpretation of Dreams. New York: Basic Books, 2010. Gunn, Thom. “Jack Straw’s Castle.” Jack Straw’s Castle. London: Faber and Faber, 1976. 48-56. ———. “On the Move.” Poetry Foundation, 1957. 3 Feb. 2020 <https://www.poetryfoundation.org/poems/57037/on-the-move>. ———. “The Annihilation of Nothing.” Poetry 93.1 (Oct. 1958): 4. 3 Feb. 2020 <https://www.poetryfoundation.org/poetrymagazine/browse?contentId=27820>. Hinton, B.J.C. The Poetry of Thom Gunn. M.A. thesis. Faculty of Arts, Birmingham University, 1975. 3 Feb. 2020 <https://etheses.bham.ac.uk/id/eprint/5366/1/Hinton1975MA.pdf>.“Negative Capability.” Glossary of Poetic Terms. Poetry Foundation, 2020. 3 Feb. 2020 <https://www.poetryfoundation.org/learn/glossary-terms/negative-capability>. Russo, Richard A. “Dream Poetry as Dream Work.” Dreaming 13.1 (Mar. 2003): 13-27. 3 Feb. 2020 <https://link.springer.com/article/10.1023/A:1022134200865>. Scobie, W.I. “Gunn in America.” London Magazine (Dec. 1977): 5-15. Shafton, Anthony. Dream Reader: Contemporary Approaches to the Understanding of Dreams. Albany: State University of New York Press, 1995. Spearing, A.C. Medieval Dream-Poetry. Cambridge: Cambridge University Press, 1976. Townley, Roderick. Night Errands: How Poets Use Dreams. Pittsburgh: University of Pittsburgh Press, 1999.

IntroductionThe walking tour is an enduring feature of cities. Fuelled by a desire to learn more about the hidden and unknown spaces of the city, the walking tour has moved beyond its historical role as tourist attraction to play a key role in the transformation of urban space through gentrification. Conversely, the walking tour has a counter-history as part of a critical urban praxis. This article reflects on historical examples, as well as our own experience of conducting Field Trip, a critical geographical walking tour through an industrial precinct in Marrickville, a suburb of Sydney that is set to undergo rapid change as a result of high-rise residential apartment construction (Gibson et al.). This precinct, known as Carrington Road, is located on the unceded land of the Cadigal and Wangal people of the Eora nation who call the area Bulanaming.Drawing on a long history of philosophical walking, many contemporary writers (Solnit; Gros; Bendiner-Viani) have described walking as a practice that can open different ways of thinking, observing and being in the world. Some have focused on the value of walking to the study of place (Hall; Philips; Heddon), and have underscored its relationship to established research methods, such as sensory ethnography (Springgay and Truman). The work of Michel de Certeau pays particular attention to the relationship between walking and the city. In particular, the concepts of tactics and strategy have been applied in a variety of ways across cultural studies, cultural geography, and urban studies (Morris). In line with de Certeau’s thinking, we view walking as an example of a tactic – a routine and often unconscious practice that can become a form of creative resistance.In this sense, walking can be a way to engage in and design the city by opposing its structures, or strategies. For example, walking in a city such as Sydney that is designed for cars requires choosing alternative paths, redirecting flows of people and traffic, and creating custom shortcuts. Choosing pedestrianism in Sydney can certainly feel like a form of resistance, and we make the argument that Field Trip – and walking tours more generally – can be a way of doing this collectively, firstly by moving in opposite directions, and secondly, at incongruent speeds to those for whom the scale and style of strategic urban development is inevitable. How such tactical walking relates to the design of cities, however, is less clear. Walking is a generally described in the literature as an individual act, while the design of cities is, at its best participatory, and always involving multiple stakeholders. This reveals a tension between the practice of walking as a détournement or appropriation of urban space, and its relationship to existing built form. Field Trip, as an example of collective walking, is one such appropriation of urban space – one designed to lead to more democratic decision making around the planning and design of cities. Given the anti-democratic, “post-political” nature of contemporary “consultation” processes, this is a seemingly huge task (Legacy et al.; Ruming). We make the argument that Field Trip – and walking tours more generally – can be a form of collective resistance to top-down urban planning.By using an open-source wiki in combination with the Internet Archive, Field Trip also seeks to collectively document and make public the local knowledge generated by walking at the frontier of gentrification. We discuss these digital choices as oppositional practice, and consider the idea of tactical media (Lovink and Garcia; Raley) in order to connect knowledge sharing with the practice of walking.This article is structured in four parts. Firstly, we provide a historical introduction to the relationship between walking tours and gentrification of global cities. Secondly, we examine the significance of walking tours in Sydney and then specifically within Marrickville. Thirdly, we discuss the Field Trip project as a citizen-led walking tour and, finally, elaborate on its role as tactical media project and offer some conclusions.The Walking Tour and Gentrification From the outset, people have been walking the city in their own ways and creating their own systems of navigation, often in spite of the plans of officialdom. The rapid expansion of cities following the Industrial Revolution led to the emergence of “imaginative geographies”, where mediated representations of different urban conditions became a stand-in for lived experience (Steinbrink 219). The urban walking tour as mediated political tactic was utilised as far back as Victorian England, for reasons including the celebration of public works like the sewer system (Garrett), and the “othering” of the working class through upper- and middle-class “slum tourism” in London’s East End (Steinbrink 220). The influence of the Situationist theory of dérive has been immense upon those interested in walking the city, and we borrow from the dérive a desire to report on the under-reported spaces of the city, and to articulate alternative voices within the city in this project. It should be noted, however, that as Field Trip was developed for general public participation, and was organised with institutional support, some aspects of the dérive – particularly its disregard for formal structure – were unable to be incorporated into the project. Our responsibility to the participants of Field Trip, moreover, required the imposition of structure and timetable upon the walk. However, our individual and collective preparation for Field Trip, as well as our collective understanding of the area to be examined, has been heavily informed by psychogeographic methods that focus on quotidian and informal urban practices (Crosby and Searle; Iveson et al).In post-war American cities, walking tours were utilised in the service of gentrification. Many tours were organised by real estate agents with the express purpose of selling devalorised inner-city real estate to urban “pioneers” for renovation, including in Boston’s South End (Tissot) and Brooklyn’s Park Slope, among others (Lees et al 25). These tours focused on a symbolic revalorisation of “slum neighbourhoods” through a focus on “high culture”, with architectural and design heritage featuring prominently. At the same time, urban socio-economic and cultural issues – poverty, homelessness, income disparity, displacement – were downplayed or overlooked. These tours contributed to a climate in which property speculation and displacement through gentrification practices were normalised. To this day, “ghetto tours” operate in minority neighbourhoods in Brooklyn, serving as a beachhead for gentrification.Elsewhere in the world, walking tours are often voyeuristic, featuring “locals” guiding well-meaning tourists through the neighbourhoods of some of the world’s most impoverished communities. Examples include the long runningKlong Toei Private Tour, through “Bangkok’s oldest and largest slum”, or the now-ceased Jakarta Hidden Tours, which took tourists to the riverbanks of Jakarta to see the city’s poorest before they were displaced by gentrification.More recently, all over the world activists have engaged in walking tours to provide their own perspective on urban change, attempting to direct the gentrifier’s gaze inward. Whilst the most confrontational of these might be the Yuppie Gazing Tour of Vancouver’s historically marginalised Downtown Eastside, other tours have highlighted the deleterious effects of gentrification in Williamsburg, San Francisco, Oakland, and Surabaya, among others. In smaller towns, walking tours have been utilised to highlight the erasure of marginalised scenes and subcultures, including underground creative spaces, migrant enclaves, alternative and queer spaces. Walking Sydney, Walking Marrickville In many cities, there are now both walking tours that intend to scaffold urban renewal, and those that resist gentrification with alternative narratives. There are also some that unwittingly do both simultaneously. Marrickville is a historically working-class and migrant suburb with sizeable populations of Greek and Vietnamese migrants (Graham and Connell), as well as a strong history of manufacturing (Castles et al.), which has been undergoing gentrification for some time, with the arts playing an often contradictory role in its transformation (Gibson and Homan). More recently, as the suburb experiences rampant, financialised property development driven by global flows of capital, property developers have organised their own self-guided walking tours, deployed to facilitate the familiarisation of potential purchasers of dwellings with local amenities and ‘character’ in precincts where redevelopment is set to occur. Mirvac, Marrickville’s most active developer, has designed its own self-guided walking tour Hit the Marrickville Pavement to “explore what’s on offer” and “chat to locals”: just 7km from the CBD, Marrickville is fast becoming one of Sydney’s most iconic suburbs – a melting pot of cuisines, creative arts and characters founded on a rich multicultural heritage.The perfect introduction, this self-guided walking tour explores Marrickville’s historical architecture at a leisurely pace, finishing up at the pub.So, strap on your walking shoes; you're in for a treat.Other walking tours in the area seek to highlight political, ecological, and architectural dimension of Marrickville. For example, Marrickville Maps: Tropical Imaginaries of Abundance provides a series of plant-led walks in the suburb; The Warren Walk is a tour organised by local Australian Labor Party MP Anthony Albanese highlighting “the influence of early settlers such as the Schwebel family on the area’s history” whilst presenting a “political snapshot” of ALP history in the area. The Australian Ugliness, in contrast, was a walking tour organised by Thomas Lee in 2016 that offered an insight into the relationships between the visual amenity of the streetscape, aesthetic judgments of an ambiguous nature, and the discursive and archival potentialities afforded by camera-equipped smartphones and photo-sharing services like Instagram. Figure 1: Thomas Lee points out canals under the street of Marrickville during The Australian Ugliness, 2016.Sydney is a city adept at erasing its past through poorly designed mega-projects like freeways and office towers, and memorialisation of lost landscapes has tended towards the literary (Berry; Mudie). Resistance to redevelopment, however, has often taken the form of spectacular public intervention, in which public knowledge sharing was a key goal. The Green Bans of the 1970s were partially spurred by redevelopment plans for places like the Rocks and Woolloomooloo (Cook; Iveson), while the remaking of Sydney around the 2000 Olympics led to anti-gentrification actions such as SquatSpace and the Tour of Beauty, an “aesthetic activist” tour of sites in the suburbs of Redfern and Waterloo threatened with “revitalisation.” Figure 2: "Tour of Beauty", Redfern-Waterloo 2016. What marks the Tour of Beauty as significant in this context is the participatory nature of knowledge production: participants in the tours were addressed by representatives of the local community – the Aboriginal Housing Company, the local Indigenous Women’s Centre, REDWatch activist group, architects, designers and more. Each speaker presented their perspective on the rapidly gentrifying suburb, demonstrating how urban space is made an remade through processes of contestation. This differentiation is particularly relevant when considering the basis for Sydney-centric walking tours. Mirvac’s self-guided tour focuses on the easy-to-see historical “high culture” of Marrickville, and encourages participants to “chat to locals” at the pub. It is a highly filtered approach that does not consider broader relations of class, race and gender that constitute Marrickville. A more intense exploration of the social fabric of the city – providing a glimpse of the hidden or unknown spaces – uncovers the layers of social, cultural, and economic history that produce urban space, and fosters a deeper engagement with questions of urban socio-spatial justice.Solnit argues that walking can allow us to encounter “new thoughts and possibilities.” To walk, she writes, is to take a “subversive detour… the scenic route through a half-abandoned landscape of ideas and experiences” (13). In this way, tactical activist walking tours aim to make visible what cannot be seen, in a way that considers the polysemic nature of place, and in doing so, they make visible the hidden relations of power that produce the contemporary city. In contrast, developer-led walking tours are singularly focussed, seeking to attract inflows of capital to neighbourhoods undergoing “renewal.” These tours encourage participants to adopt the position of urban voyeur, whilst activist-led walking tours encourage collaboration and participation in urban struggles to protect and preserve the contested spaces of the city. It is in this context that we sought to devise our own walking tour – Field Trip – to encourage active participation in issues of urban renewal.In organising this walking tour, however, we acknowledge our own entanglements within processes of gentrification. As designers, musicians, writers, academics, researchers, venue managers, artists, and activists, in organising Field Trip, we could easily be identified as “creatives”, implicated in Marrickville’s ongoing transformation. All of us have ongoing and deep-rooted connections to various Sydney subcultures – the same subcultures so routinely splashed across developer advertising material. This project was borne out of Frontyard – a community not-just-art space, and has been supported by the local Inner West Council. As such, Field Trip cannot be divorced from the highly contentious processes of redevelopment and gentrification that are always simmering in the background of discussions about Marrickville. We hope, however, that in this project we have started to highlight alternative voices in those redevelopment processes – and that this may contribute towards a “method of equality” for an ongoing democratisation of those processes (Davidson and Iveson).Field Trip: Urban Geographical Enquiry as Activism Given this context, Field Trip was designed as a public knowledge project that would connect local residents, workers, researchers, and decision-makers to share their experiences living and working in various parts of Sydney that are undergoing rapid change. The site of our project – Carrington Road, Marrickville in Sydney’s inner-west – has been earmarked for major redevelopment in coming years and is quickly becoming a flashpoint for the debates that permeate throughout the whole of Sydney: housing affordability, employment accessibility, gentrification and displacement. To date, public engagement and consultation regarding proposed development at Carrington Road has been limited. A major landholder in the area has engaged a consultancy firm to establish a community reference group (CRG) the help guide the project. The CRG arose after public outcry at an original $1.3 billion proposal to build 2,616 units in twenty towers of up to 105m in height (up to thirty-five storeys) in a predominantly low-rise residential suburb. Save Marrickville, a community group created in response to the proposal, has representatives on this reference group, and has endeavoured to make this process public. Ruming (181) has described these forms of consultation as “post-political,” stating thatin a universe of consensual decision-making among diverse interests, spaces for democratic contest and antagonistic politics are downplayed and technocratic policy development is deployed to support market and development outcomes.Given the notable deficit of spaces for democratic contest, Field Trip was devised as a way to reframe the debate outside of State- and developer-led consultation regimes that guide participants towards accepting the supposed inevitability of redevelopment. We invited a number of people affected by the proposed plans to speak during the walking tour at a location of their choosing, to discuss the work they do, the effect that redevelopment would have on their work, and their hopes and plans for the future. The walking tour was advertised publicly and the talks were recorded, edited and released as freely available podcasts. The proposed redevelopment of Carrington Road provided us with a unique opportunity to develop and operate our own walking tour. The linear street created an obvious “circuit” to the tour – up one side of the road, and down the other. We selected speakers based on pre-existing relationships, some formed during prior rounds of research (Gibson et al.). Speakers included a local Aboriginal elder, a representative from the Marrickville Historical Society, two workers (who also gave tours of their workplaces), the Lead Heritage Adviser at Sydney Water, who gave us a tour of the Carrington Road pumping station, and a representative from the Save Marrickville residents’ group. Whilst this provided a number of perspectives on the day, regrettably some groups were unrepresented, most notably the perspective of migrant groups who have a long-standing association with industrial precincts in Marrickville. It is hoped that further community input and collaboration in future iterations of Field Trip will address these issues of representation in community-led walking tours.A number of new understandings became apparent during the walking tour. For instance, the heritage-listed Carrington Road sewage pumping station, which is of “historic and aesthetic significance”, is unable to cope with the proposed level of residential development. According to Philip Bennett, Lead Heritage Adviser at Sydney Water, the best way to maintain this piece of heritage infrastructure is to keep it running. While this issue had been discussed in private meetings between Sydney Water and the developer, there is no formal mechanism to make this expert knowledge public or accessible. Similarly, through the Acknowledgement of Country for Field Trip, undertaken by Donna Ingram, Cultural Representative and a member of the Metropolitan Local Aboriginal Land Council, it became clear that the local Indigenous community had not been consulted in the development proposals for Carrington Road. This information, while not necessary secret, had also not been made public. Finally, the inclusion of knowledgeable local workers whose businesses are located on Carrington Road provided an insight into the “everyday.” They talked of community and collaboration, of site-specificity, the importance of clustering within their niche industries, and their fears for of displacement should redevelopment proceed.Via a community-led, participatory walking tour like Field Trip, threads of knowledge and new information are uncovered. These help create new spatial stories and readings of the landscape, broadening the scope of possibility for democratic participation in cities. Figure 3: Donna Ingram at Field Trip 2018.Tactical Walking, Tactical Media Stories connected to walking provide an opportunity for people to read the landscape differently (Mitchell). One of the goals of Field Trip was to begin a public knowledge exchange about Carrington Road so that spatial stories could be shared, and new readings of urban development could spread beyond the confines of the self-contained tour. Once shared, this knowledge becomes a story, and once remixed into existing stories and integrated into the way we understand the neighbourhood, a collective spatial practice is generated. “Every story is a travel story – a spatial practice”, says de Certeau in “Spatial Stories”. “In reality, they organise walks” (72). As well as taking a tactical approach to walking, we took a tactical approach to the mediation of the knowledge, by recording and broadcasting the voices on the walk and feeding information to a publicly accessible wiki. The term “tactical media” is an extension of de Certeau’s concept of tactics. David Garcia and Geert Lovink applied de Certeau’s concept of tactics to the field of media activism in their manifesto of tactical media, identifying a class of producers who amplify temporary reversals in the flow of power by exploiting the spaces, channels and platforms necessary for their practices. Tactical media has been used since the late nineties to help explain a range of open-source practices that appropriate technological tools for political purposes. While pointing out the many material distinctions between different types of tactical media projects within the arts, Rita Raley describes them as “forms of critical intervention, dissent and resistance” (6). The term has also been adopted by media activists engaged in a range of practices all over the world, including the Tactical Technology Collective. For Field Trip, tactical media is a way of creating representations that help navigate neighbourhoods as well as alternative political processes that shape them. In this sense, tactical representations do not “offer the omniscient point of view we associate with Cartesian cartographic practice” (Raley 2). Rather these representations are politically subjective systems of navigation that make visible hidden information and connect people to the decisions affecting their lives. Conclusion We have shown that the walking tour can be a tourist attraction, a catalyst to the transformation of urban space through gentrification, and an activist intervention into processes of urban renewal that exclude people and alternative ways of being in the city. This article presents practice-led research through the design of Field Trip. By walking collectively, we have focused on tactical ways of opening up participation in the future of neighbourhoods, and more broadly in designing the city. 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