Journal articles on the topic 'FOS Based Control'
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1
Wong, Pak Kin, Chi Man Vong, Xiang Hui Gao, and Ka In Wong. "Adaptive Control Using Fully Online Sequential-Extreme Learning Machine and a Case Study on Engine Air-Fuel Ratio Regulation." Mathematical Problems in Engineering 2014 (2014): 1–11. http://dx.doi.org/10.1155/2014/246964.
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Most adaptive neural control schemes are based on stochastic gradient-descent backpropagation (SGBP), which suffers from local minima problem. Although the recently proposed regularized online sequential-extreme learning machine (ReOS-ELM) can overcome this issue, it requires a batch of representative initial training data to construct a base model before online learning. The initial data is usually difficult to collect in adaptive control applications. Therefore, this paper proposes an improved version of ReOS-ELM, entitled fully online sequential-extreme learning machine (FOS-ELM). While retaining the advantages of ReOS-ELM, FOS-ELM discards the initial training phase, and hence becomes suitable for adaptive control applications. To demonstrate its effectiveness, FOS-ELM was applied to the adaptive control of engine air-fuel ratio based on a simulated engine model. Besides, controller parameters were also analyzed, in which it is found that large hidden node number with small regularization parameter leads to the best performance. A comparison among FOS-ELM and SGBP was also conducted. The result indicates that FOS-ELM achieves better tracking and convergence performance than SGBP, since FOS-ELM tends to learn the unknown engine model globally whereas SGBP tends to “forget” what it has learnt. This implies that FOS-ELM is more preferable for adaptive control applications.
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Shinoki, Aki, and Hiroshi Hara. "Dietary fructo-oligosaccharides improve insulin sensitivity along with the suppression of adipocytokine secretion from mesenteric fat cells in rats." British Journal of Nutrition 106, no. 8 (June 2, 2011): 1190–97. http://dx.doi.org/10.1017/s000711451100167x.
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Short-chain fructo-oligosaccharides (FOS) are known to have beneficial effects on health. However, the effects of FOS on insulin resistance have not been fully clarified. We observed the effects of FOS feeding on insulin sensitivity and adipocytokine release from abdominal adipocytes in weaning rats. Male Sprague–Dawley rats, 3 weeks old, were divided into three groups and fed a sucrose-based American Institute of Nutrition (AIN)-93 growth diet (control), the control diet containing 5 % FOS for 5 weeks (FOS-5wk) or the control diet for 2 weeks followed by the 5 % FOS diet for 3 weeks (FOS-3wk). Tail blood was collected after fasting for 9 h on day 33 of feeding, and glucose and insulin levels were measured. On the last day, rats were anaesthetised and killed after the collection of aortic blood. Small- and large-intestinal mesenteric fat tissues were immediately excised, and the release of adiponectin, leptin and TNF-α was evaluated from the subsequently isolated adipocytes. The weight of the large-intestinal mesenteric fat, fasting blood insulin level and homeostatic model assessment for insulin resistance decreased in a time-dependent manner, and were much lower in the FOS-5wk group than in the control group. These values were correlated with aortic blood leptin levels. The secretion rate of leptin from the isolated mesenteric adipocytes in the small intestine, but not in the large intestine, was lower in the FOS-fed groups than in the control group. In conclusion, FOS feeding improved insulin sensitivity accompanied by the reduction in large-intestinal fat mass and leptin secretion from the mesenteric adipocytes of the small intestine.
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KUTILEK, PATRIK, ONDREJ CAKRT, VLADIMIR SOCHA, and KAREL HANA. "VOLUME OF CONVEX HULL: A TECHNIQUE FOR QUANTIFYING HUMAN POSTURAL STABILITY." Journal of Mechanics in Medicine and Biology 16, no. 02 (March 2016): 1650013. http://dx.doi.org/10.1142/s0219519416500135.
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Many disorders, such as nervous system disorders, can affect orientation of the body segments in 3D space negatively. Patients with these disorders often show body segments instability during stance tasks. Nowadays, 3-axis gyroscopes are about to be used to measure postural stability. The main objective of the paper is to describe a method which would be suitable for quantifying postural stability and 3D movement as a whole using a cheap 3-axis gyroscope. New method based on the volume of a 3D convex hull (CH) obtained by plotting pitch, roll and yaw angles versus each other was proposed for quantitative evaluation of 3D trunk sway. The sway was measured while patients with degenerative cerebellar disorder (Pts) and eleven healthy subjects (HSs) performed quiet stance on a firm surface (FiS) and foam surface (FoS) with open eyes (OE) and closed eyes (CE). The CH was used to identify differences in balance control, and there were significant differences found between the two groups. The median (Mdn) of the volume of Pts with OE on FiS is four times larger than the Mdn of the volume of HS with OE on FiS. The Mdn of the volume of Pts with CE on FoS is 80 times larger than the Mdn of the volume of HS with CE on FoS. It was therefore found that the volume of CH is suitable for quantifying postural sway and identifying differences in balance control.
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Lobulu, John, Hussein Shimelis, Mark D. Laing, Arnold Angelo Mushongi, and Admire Isaac Tichafa Shayanowako. "Characterization of Maize Genotypes (Zea mays L.) for Resistance to Striga asiatica and S. hermonthica and Compatibility with Fusarium oxysporum f. sp. strigae (FOS) in Tanzania." Agronomy 11, no. 5 (May 18, 2021): 1004. http://dx.doi.org/10.3390/agronomy11051004.
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Striga species cause significant yield loss in maize varying from 20 to 100%. The aim of the present study was to screen and identify maize genotypes with partial resistance to S. hermonthica (Sh) and S. asiatica (Sa) and compatible with Fusarium oxysporum f. sp. strigae (FOS), a biocontrol agent. Fifty-six maize genotypes were evaluated for resistance to Sh and Sa, and FOS compatibility. Results showed that FOS treatment significantly (p < 0.001) enhanced Striga management compared to the untreated control under both Sh and Sa infestations. The mean grain yield was reduced by 19.13% in FOS-untreated genotypes compared with a loss of 13.94% in the same genotypes treated with FOS under Sh infestation. Likewise, under Sa infestation, FOS-treated genotypes had a mean grain yield reduction of 18% while untreated genotypes had a mean loss of 21.4% compared to the control treatment. Overall, based on Striga emergence count, Striga host damage rating, grain yield and FOS compatibility, under Sh and Sa infestations, 23 maize genotypes carrying farmer preferred traits were identified. The genotypes are useful genetic materials in the development of Striga-resistant cultivars in Tanzania and related agro-ecologies.
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Yan, Fulong, Changshun Yuan, Chao Li, and Xiong Deng. "FOSquare: A Novel Optical HPC Interconnect Network Architecture Based on Fast Optical Switches with Distributed Optical Flow Control." Photonics 8, no. 1 (January 4, 2021): 11. http://dx.doi.org/10.3390/photonics8010011.
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Interconnecting networks adopting Fast Optical Switches (FOS) can achieve high bandwidth, low latency, and low power consumption. We propose and demonstrate a novel interconnecting topology based on FOS (FOSquare) with distributed fast flow control which is suitable for HPC infrastructures. We also present an Optimized Mapping (OPM) algorithm that maps the most communication-related processes inside a rack. We numerically investigate and compare the network performance of FOSquare with Leaf-Spine under real traffic traces collected by running multiple applications (CG, MG, MILC, and MINI_MD) in an HPC infrastructure. The numerical results show that the FOSquare can reduce >10% latency with respect to Leaf-Spine under the scenario of 16 available cores.
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Li, Bingxin, Yaowei Liu, and Xin Zhao. "Robust H∞ Control for Fractional Order Systems with Order α (0 < α < 1)." Fractal and Fractional 6, no. 2 (February 2, 2022): 86. http://dx.doi.org/10.3390/fractalfract6020086.
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In the paper, H∞ and robust H∞ control for fractional order systems (FOS) with order 0<α<1 are studied. Firstly, necessary and sufficient conditions of H∞ control and state feedback controller design are proposed. Then, robust H∞ control for FOS with uncertainty is studied, and state feedback controller is designed. These conditions are based on linear matrix inequalities (LMI) and can be easily solved by the LMI toolbox. Finally, the effectiveness of these conditions is verified by two numerical examples.
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Machado, Renato Assis, Lilianny Querino Rocha de Oliveira, Ana Lúcia Carrinho Ayroza Rangel, Silvia Regina de Almeida Reis, Rafaela Scariot, Daniella Reis Barbosa Martelli, Hercílio Martelli-Júnior, and Ricardo D. Coletta. "Brazilian Multiethnic Association Study of Genetic Variant Interactions among FOS, CASP8, MMP2 and CRISPLD2 in the Risk of Nonsyndromic Cleft Lip with or without Cleft Palate." Dentistry Journal 11, no. 1 (December 26, 2022): 7. http://dx.doi.org/10.3390/dj11010007.
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Associations of CRISPLD2 (cysteine-rich secretory protein LCCL domain containing 2) and genes belonging to its activation pathway, including FOS (Fos proto-oncogene), CASP8 (caspase 8) and MMP2 (matrix metalloproteinase 2), with nonsyndromic orofacial cleft risk, have been reported, but the results are yet unclear. The aim of this study was to evaluate single nucleotide polymorphisms (SNPs) in FOS, CASP8 and MMP2 and to determine their SNP-SNP interactions with CRISPLD2 variants in the risk of nonsyndromic cleft lip with or without cleft palate (NSCL±P) in the Brazilian population. The SNPs rs1046117 (FOS), rs3769825 (CASP8) and rs243836 (MMP2) were genotyped using TaqMan allelic discrimination assays in a case-control sample containing 801 NSCL±P patients (233 nonsyndromic cleft lip only (NSCLO) and 568 nonsyndromic cleft lip and palate (NSCLP)) and 881 healthy controls via logistic regression analysis adjusted for the effects of sex and genomic ancestry proportions with a multiple comparison p value set at ≤0.01. SNP-SNP interactions with rs1546124, rs8061351, rs2326398 and rs4783099 in CRISPLD2 were performed with the model-based multifactor dimensionality reduction test complemented with a 1000 permutation-based strategy. Although the association between FOS rs1046117 and risk of NSCL±P reached only nominal p values, NSCLO risk was significantly higher in carriers of the FOS rs1046117 C allele (OR: 1.28, 95% CI: 1.10–1.64, p = 0.004), TC heterozygous genotype (OR: 1.59, 95% CI: 1.16–2.18, p = 0.003), and in the dominant model (OR: 1.50, 95% CI: 1.10–2.02, p = 0.007). Individually, no significant associations between cleft risk and the SNPs in CASP8 and MMP2 were observed. SNP-SNP interactions involving CRISPLD2 variants and rs1046117 (FOS), rs3769825 (CASP8) and rs243836 (MMP2) yielded several significant p values, mostly driven by FOS rs1046117 and CASP8 rs3769825 in NSCL±P, FOS rs1046117 in NSCLO and CRISPLD2 rs8061351 in NSCLP. Our study is the first in the Brazilian population to reveal the association of FOS rs1046117 with NSCLO risk, and to support that CRISPLD2, CASP8, FOS and MMP2 interactions may be related to the pathogenesis of this common craniofacial malformation.
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Leng, Jinsong, and A. Asundi. "Active vibration control system of smart structures based on FOS and ER actuator." Smart Materials and Structures 8, no. 2 (January 1, 1999): 252–56. http://dx.doi.org/10.1088/0964-1726/8/2/011.
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Hsieh, Tzu-Yun, Pi-Lien Hung, Ting-Yu Su, and Syu-Jyun Peng. "Graph Theory-Based Electroencephalographic Connectivity via Phase-Locking Value and Its Association with Ketogenic Diet Responsiveness in Patients with Focal Onset Seizures." Nutrients 14, no. 21 (October 23, 2022): 4457. http://dx.doi.org/10.3390/nu14214457.
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Ketogenic diets (KDs) are a promising alternative therapy for pediatric refractory epilepsy. Several predictors of KD responsiveness have been identified, including biochemical parameters, seizure types, and electroencephalography (EEG) examinations. We hypothesized that graph theory-based EEG functional connectivity could explain KD responses in patients presenting focal onset seizure (FOS). A total of 17 patients aged 0–30 years old with focal onset seizures (FOS) were recruited as a study group between January 2015 and July 2021. Twenty age-matched children presenting headache with no intracranial complications nor other medical issues were enrolled as a control group. Data were obtained at baseline and at 12 months after initiating KD therapy (KDT) using the child behavior checklist (CBCL) and brain functional connectivity parameters based on phase-locking value from 19 scalp EEG signals, including nodal strength, global efficiency, clustering coefficient, and betweenness centrality. Compared with age-matched controls, patients presenting FOS with right or bilateral EEG lateralization presented higher baseline functional connectivity, including parameters such as global efficiency, mean cluster coefficient and mean nodal strength in the delta and beta frequency bands. In patients presenting FOS with right or bilateral EEG lateralization, the global efficiency of functional connectivity parameters in the delta and theta frequency bands was significantly lower at 12 months after KDT treatment than before KDT. Those patients also presented a significantly lower mean clustering coefficient and mean nodal strength in the theta frequency band at 12 months after KDT treatment. Changes in brain functional connectivity were positively correlated with social problems, attention, and behavioral scores based on CBCL assessments completed by parents. This study provides evidence that KDT might be beneficial in the treatment of patients with FOS. Graph theoretic analysis revealed that the observed effects were related to decreased functional connectivity, particularly in terms of global efficiency. Our findings related to brain connectivity revealed lateralization to the right (non-dominant) hemisphere; however, we were unable to define the underlying mechanism. Our data revealed that in addition to altered brain connectivity, KDT improved the patient’s behavior and emotional state.
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Grakovski, Alexander, Alexey Pilipovec, Igor Kabashkin, and Elmars Petersons. "Weight-in-Motion Estimation Based on Reconstruction of Tyre Footprint’s Geometry by Group of Fibre Optic Sensors." Transport and Telecommunication Journal 15, no. 2 (June 1, 2014): 97–110. http://dx.doi.org/10.2478/ttj-2014-0009.
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Abstract The problem of measuring road vehicle’s weight-in-motion (WIM) is important for overload enforcement, road maintenance planning and cargo fleet managing, control of the legal use of the transport infrastructure, road surface protection from the early destruction and for the safety on the roads. The fibre-optic sensors (FOS) functionality is based on the changes in the parameters of the optical signal due to the deformation of the optical fibre under the weight of the crossing vehicle. A fibre-optic sensor responds to the deformation, therefore for WIM measurements it is necessary to estimate the impact area of a wheel on the working surface of the sensor called tyre footprint. This information is used further for the estimation of the vehicle wheel’s speed, contact width, length, and, finally, axle’s weight while in motion. Recorded signals from a truck passing over a group of FOS with various speeds and known weight are used as an input data. The results of the several laboratory and field experiments with FOS, e.g. load characteristics according to the temperature, contact surface width and loading speed impact, are provided here. The method of initial signal deconvolution on symmetric and asymmetric components provides the chance to approximate geometric size of tyre surface footprint as well as calculate weight on each wheel separately. The examples of the estimation of a truck speed, tyre contact surface footprint parameters using FOS signals are discussed in this article.
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Huang, Haocai, Chenyun Zhang, Weiwei Ding, Xinke Zhu, Guiqing Sun, and Hangzhou Wang. "Design of the Depth Controller for a Floating Ocean Seismograph." Journal of Marine Science and Engineering 8, no. 3 (March 3, 2020): 166. http://dx.doi.org/10.3390/jmse8030166.
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Floating ocean seismograph (FOS) is a vertical underwater vehicle used to detect ocean earthquakes by observing P waves at teleseismic distances in the oceans. With the requirements of rising to the surface and transmitting data to the satellite in real time and diving to the desired depth and recording signals, the depth control of FOS needs to be zero overshoot and accurate with fast response. So far, it remains challenging to implement such depth control due to the variation of buoyancy caused by the seawater density varying with the depth. The deeper the water is, the greater the impacts on buoyancy are. To tackle it, a fuzzy sliding mode controller considering the influence of seawater density change is proposed and simulated in MATLAB/SIMULINK based on the variable buoyancy system and state space function of FOS. Compared with proportional-integral-derivative (PID) controller, fuzzy PID controller and sliding mode controller, the simulation results indicate that the proposed controller shows its superiority regardless of the disturbing force. Its advantages include smaller steady-state error, faster response time, smaller system chatter, and well robustness. This proves that the designed fuzzy sliding mode controller is able to meet the working requirements and thus, lays a foundation for FOS application.
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Malhi, Saima Mahmood, Huma Jawed, Farina Hanif, Nadeem Ashraf, Farhat Zubair, Bina S. Siddiqui, Sabira Begum, Nurul Kabir, and Shabana Usman Simjee. "Modulation of c-Fos and BDNF Protein Expression in Pentylenetetrazole-Kindled Mice following the Treatment with Novel Antiepileptic Compound HHL-6." BioMed Research International 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/876712.
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Brain-derived neurotrophic factor (BDNF) and c-Fos are shown to promote epileptogenesis and are taken as a marker of neuronal activity. The present study investigated the expression of BDNF and c-Fos in mice brain with pentylenetetrazol- (PTZ-) induced generalized seizure and evaluated the effect of novel tryptamine derivative HHL-6 on the expression of these two markers. The subconvulsive dose of PTZ (50 mg/kg) was administered on alternate days in the experimental groups until the seizure scores 4-5 developed in the PTZ-control group. At the end of each experiment, animals were sacrificed, brain samples were collected and cryosectioned, and immunohistochemical analysis of BDNF and c-Fos protein was performed. Data obtained from two sections per mouse (n=12animals/group) is presented as means ± S.E.M. The test compound HHL-6 demonstrated a potent anticonvulsant activity in the PTZ-induced seizure in mice. Significant reduction in the BDNF (P<0.003) and c-Fos (P<0.01) protein expression was observed in the HHL-6 treated group. Based on these results we suggest that one of the possible mechanisms of HHL-6 to inhibit epileptogenesis might be due to its controlling effect on the cellular and molecular expression of the factors that contribute to the development of epileptogenic plasticity in the CNS.
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Fujiwara, Reiko, Jun Watanabe, and Kei Sonoyama. "Assessing changes in composition of intestinal microbiota in neonatal BALB/c mice through cluster analysis of molecular markers." British Journal of Nutrition 99, no. 6 (June 2008): 1174–77. http://dx.doi.org/10.1017/s0007114507862349.
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The present study introduced a molecular biological approach to demonstrate changes in the composition of intestinal microbiota in neonatal mice. Female BALB/c mice were fed either a control diet or a diet supplemented with fructo-oligosaccharide (FOS) at 50 g/kg diet, and then mated to male mice. A cultivation-independent approach, denaturing gradient gel electrophoresis (DGGE) of the PCR-amplified 16S rRNA gene, was performed to characterise changes in intestinal microbial populations in pups at 0, 7, 14 and 21 d old and their dams. Comparisons of DGGE profiles were performed using the Dice similarity coefficient and the unweighted pair group method with arithmetic mean (UPGMA) cluster analysis based on numbers, positions and intensities of bands. DGGE profiles differed between dams fed control and FOS-supplemented diets. Although profiles in pups on the day of birth showed a high similarity with dams, profiles in 7-d-old pups differed from dams and showed high similarity to littermates. In 14- and 21-d-old pups, profiles again showed high similarity with dams. DGGE profiles in pups were divided into two large clusters of control and FOS-supplemented diet groups in the range of 0- to 21-d-old, suggesting modulation of intestinal microbiota in infants by manipulation of microbiota in dams. The present study shows a useful technique for demonstrating changes in intestinal microbiota and provides a mouse model for modulation of intestinal microbiota in neonatal life.
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Be´nard, C., and M. M. Rosset-Loue¨rat. "Identification of a Simulation Model and a Control Model for a Coke Oven Battery Operating Under Transient Conditions." Journal of Dynamic Systems, Measurement, and Control 114, no. 3 (September 1, 1992): 444–53. http://dx.doi.org/10.1115/1.2897367.
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This work addresses the problem of identifying a model of a coke oven plant that would allow its control under unsteady operating conditions. Knowing that coal thermal kinetics in the oven are the most significant factors affecting the resulting coke quality, considering that control is global since all the heating chambers of a battery are supplied by the same feeder, and that constraints arising in the process involve local and global variables, our study is based on the use of two dynamic models: a detailed elementary model of one oven and a global model of the plant. The first model is deduced from a detailed physical model and gives, for an elementary oven, the relationship between the heating power input and the coke temperature output or coke-gas yield indicator. It is tested by comparison with measurement performed at the Sollac-Fos plant. The second model is a lumped state representation that describes, with a few state variables, the evolution of the two batteries. Its parameters are identified with the help of measurements performed at the Sollac-Fos plant.
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Mobini Kesheh, Mina, Mohadeseh Barazandeh, Amir Kaffashi, Mohammad Kazem Shahkarami, and Seyed Alireza Nadji. "Genetic Diversity of HPV 16 and HPV 18 Based on Partial Long Control Region in Iranian Women." Canadian Journal of Infectious Diseases and Medical Microbiology 2022 (January 25, 2022): 1–7. http://dx.doi.org/10.1155/2022/4759871.
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Background. Human papillomavirus (HPV) 16 and HPV 18 account for 75% of all cervical cancers. The L1 gene, encoding the major surface protein (MSP), is used to classify HPV types (lineages and sublineages), genotypes, and intratypic variants. Therefore, this study aimed to investigate the lineages, sublineages, genetic variabilities, and mutation effects on transcription factor binding sites by using partial sequences of the HPV 16 and HPV 18 long control regions (LCRs) in these samples. Materials and Methods. After DNA isolation from 56 positive samples, the LCR of HPV 16 and HPV 18 were amplified using specific primers, and phylogenetic trees were drawn through MEGA X. Compared to the reference sequences, single nucleotide polymorphisms (SNPs) were identified. The transcription binding sites were also evaluated using the online PROMO database. Results. The LCRs of 52 samples were successfully sequenced. Overall, 81.58% of all HPV 16 variants belonged to the D1 sublineage, followed by A4 (13.16%), A1 (2.63%), and C1 (2.63%) sublineages. All HPV 18 isolates belonged to A sublineage, 92.85% to A3 sublineage, and 7.15% to A4 sublineage. Out of 27 SNPs in the HPV 16 LCR, A7382T, T7384G, C7387T, C7393G, A7431G, T7448C, and C7783A were HPV 16-specific. Also, among 14 SNPs in the HPV 18 LCR, C7577A and A7943T were not previously reported. An insertion (C) between 7432 and 7433 positions was identified in all studied HPV 16 variants. Besides, most of the HPV 16 mutations were embedded in the YY1, TFIID, Oct-2, and NF-1 binding sites, while c-Fos and MBF1, as the most common binding sites, were affected by HPV 18 LCR mutations. Conclusion. The present results showed that D1 and A3 were the dominant sublineages of HPV 16 and HPV 18, respectively. Therefore, women infected with these variants need to be examined in further longitudinal studies to obtain more information about the oncogenic potential of these dominant variants in Iran. Besides, YY1, TFIID, Oct-2, NF-1, c-Fos, and MBF1 were the most frequent binding sites, which were influenced by the mutations.
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Hira, Tohru, Asuka Ikee, Yuka Kishimoto, Sumiko Kanahori, and Hiroshi Hara. "Resistant maltodextrin promotes fasting glucagon-like peptide-1 secretion and production together with glucose tolerance in rats." British Journal of Nutrition 114, no. 1 (February 11, 2015): 34–42. http://dx.doi.org/10.1017/s0007114514004322.
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Glucagon-like peptide-1 (GLP-1), which is produced and released from enteroendocrine L cells, plays pivotal roles in postprandial glycaemia. The ingestion of resistant maltodextrin (RMD), a water-soluble non-digestible saccharide, improves the glycaemic response. In the present study, we examined whether the continuous feeding of RMD to rats affected GLP-1 levels and glycaemic control. Male Sprague–Dawley rats (6 weeks of age) were fed an American Institute of Nutrition (AIN)-93G-based diet containing either cellulose (5 %) as a control, RMD (2·5 or 5 %), or fructo-oligosaccharides (FOS, 2·5 or 5 %) for 7 weeks. During the test period, an intraperitoneal glucose tolerance test (IPGTT) was performed after 6 weeks. Fasting GLP-1 levels were significantly higher in the 5 % RMD group than in the control group after 6 weeks. The IPGTT results showed that the glycaemic response was lower in the 5 % RMD group than in the control group. Lower caecal pH, higher caecal tissue and content weights were observed in the RMD and FOS groups. Proglucagon mRNA levels were increased in the caecum and colon of both RMD and FOS groups, whereas caecal GLP-1 content was increased in the 5 % RMD group. In addition, a 1 h RMD exposure induced GLP-1 secretion in an enteroendocrine L-cell model, and single oral administration of RMD increased plasma GLP-1 levels in conscious rats. The present study demonstrates that continuous ingestion of RMD increased GLP-1 secretion and production in normal rats, which could be stimulated by its direct and indirect (enhanced gut fermentation) effects on GLP-1-producing cells, and contribute to improving glucose tolerance.
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Lyu, Pengcheng, Robert Settlage, and Honglin Jiang. "192 Identification of Fos and FosB as Transcriptional Regulators of Bovine Satellite Cell Differentiation." Journal of Animal Science 99, Supplement_3 (October 8, 2021): 103. http://dx.doi.org/10.1093/jas/skab235.186.
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Abstract Transcription factors (TFs) are key regulators of gene expression during cell differentiation. Four TFs including Myf5, MyoD, MyoG and Myf6 have been identified as key myogenic regulatory factors (MYFs) that regulate gene transcription during myogenesis. Satellite cells (SCs) are the myogenic precursor cells in adult skeletal muscle. The objective of this study was to identify additional TFs that control the differentiation of bovine satellite cells. Bovine satellite cells (bSCs) were isolated from 4 crossbred steers and were initially cultured in growth medium for 12 days to expand and then in differentiation medium for 48 hours to differentiate. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed to identify chromatin regions marked with acetylation of histone H3 on lysine 27 (H3K27ac). This ChIP-seq analysis revealed 3,348 and 38,800 H3K27ac-associated chromatin regions in bSCs before and after differentiation, respectively. A motif enrichment analysis of the H3K27ac-marked chromatin regions from the differentiated bSCs indicated the enrichment of binding sites for the 4 MYFs and many other TFs including Fos and FosB. RNA-sequencing revealed the upregulation of Fos and FosB mRNAs in bSCs from growth to differentiation. To verify the roles of Fos and FosB in bSC differentiation, their expressions in bSCs were reduced by siRNA-induced knockdown. Based on qRT-PCR analyses, expressions of MYH2, MYH3, MYOG, and CKM mRNAs, which were selected as markers of muscle cell differentiation, were increased (P < 0.05) in bSCs from growth to differentiation, but the increases in at least three of them were reversed (P < 0.05) by Fos or FosB knockdown. Taken together, these results establish Fos and FosB as transcriptional regulators of bovine satellite cell differentiation.
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Kobylyukh, I. B., Y. S. Stravskiy, V. Y. Stefanyk, and Y. Y. Kostyshyn. "Влив препарату «Фос-Бевіт» на вміст холестеролу та концентрацію сечової кислоти в організмі корів та перебіг у них післяотельного періоду." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 19, no. 77 (March 4, 2017): 204–7. http://dx.doi.org/10.15421/nvlvet7744.
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The tendency of spreading postnatal complications of dairy herd in modern conditions requires the use of new tools and prevention schemes obstetric pathology in cows. The aim of the study was to explore the impact of the preparation «Fos-Bevit» on cholesterol content and the concentration of uric acid in the organism of cows and the course of their postnatal period. The experiment conducted in LLC «Agroprodservice-Invest» of Kozivskyi district, Ternopil region on cows of Ukrainian black and white dairy cattle breed. In order to design the scheme of the preparation usage with immunostimulating properties we used «Fos-Bevit» – a complex preparation of production of LLC SPC «Brovafarma» based on butafosfan and three group B vitamins (nicotinamide, folic acid, cyanocobalamin), which apply to cattle metabolic disorders, vitamin deficiency, as a stimulant and a tonic preparation to boost the body's resistance to diseases of various etiologies, improving the growth and development. Having used the preparation «Fos-Bevit» intramuscular in dose of 0,3 ml/10 kg of body mass for 1.5 day after calving in the blood of cows increases the cholesterol content by 10.69% (P ≤ 0.05) and decreases by 14.27% (P ≤ 0.05) the concentration of uric acid, which contributes to the activation of the body's antioxidant system and metabolism. The preparation «Fos-Bevit» positively influence the after born period that can be observed the decrease of service period by 75 days (Р ≤ 0,01) and decrease of the index of insemination for 0.4 item in comparison with cows of the control group in which subclinical endometritis and mastitis were diagnosed. The preparation «Fos-Bevit» is recommended for including into the technological scheme of prophylaxis of afterbirth diseases in cows.
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Butakov, E. I., and L. D. Shamanskaya. "The efficacy of FOS and Fitoverm drugs against Ixodes ticks." Russian Journal of Parasitology 15, no. 1 (March 11, 2021): 85–91. http://dx.doi.org/10.31016/1998-8435-2021-15-1-85-91.
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The purpose of the research is studying the efficacy of FOS and Fitoverm drugs against ixodid ticks.Materials and methods. The study objects were ixodid tick species Ixodes persulcatus (Schulze, 1930), Dermacentor reticulatus (Hermann, 1804) and D. marginatus (Schulzer, 1776) in the imago stage. Ticks were collected in the environment of the Altai plains and mountains from vegetation to the flag under the methodology. The efficacy of drug acaricidal activity was assessed according to the methodological guide. In the laboratory, the ticks were placed in a chamber at a temperature of 7 оC. The effect of drugs was assessed by observation every hour for 2-3 days. Live ticks (20 individuals) were contacted with the drug by immersion three times in the working solution in a permeable fabric container. Neostomosan was used as control. Further tests of FOS and Fitoverm were carried out in the field environment. Briz 25% e.c. was used as control. The plots on which the number of Ixodes had previously been recorded was treated using a sprayer on June 1-2 in the morning. The number of live ticks in the plots was counted 1-2 days after the treatment. The biochemical composition of grass stand on various treated grounds was studied according to standard methods. The results were processed statistically.Results and discussion. The Siberian Scientific-Research Institute of Horticulture has developed a series of environmentally friendly drugs based on natural biologically active substances that have shown high efficacy against Ixodes. These are FOS and Fitoverm, which provided a 100% lethal effect against Ixodes in working concentrations of 3–4% and 0.1–0.3% under laboratory conditions. Treatment with Fitoverm 0.3%, FOS 3% and synthetic industrial drug Briz 25% of e.c. 0.75% of grass stand at the stage of grazing for cattle in pasture conditions statistically significantly reduces the number of ticks by 71–79%.
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Bingham, Brenda, Nancy X. R. Wang, Leyla Innala, and Victor Viau. "Postnatal Aromatase Blockade Increases c-fos mRNA Responses to Acute Restraint Stress in Adult Male Rats." Endocrinology 153, no. 4 (February 7, 2012): 1603–8. http://dx.doi.org/10.1210/en.2011-1749.
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Recent evidence suggests that the aromatization of testosterone to estrogen is important for the organizing effects of neonatal testosterone on neuroendocrine responses to acute challenges. However, the extent to which neonatal inhibition of aromatase alters the stress-induced activation of neural pathways has not been examined. Here we assessed central patterns of c-fos mRNA induced by 30 min of restraint in 65-d-old adult male rats that were implanted with sc capsules of the aromatase inhibitor 1,4,6-androstatriene-3,17-dione (ATD), introduced within 12 h of birth and removed on d 21 of weaning. Neonatal ATD decreased the expression of arginine vasopressin within extrahypothalamic regions in adults, confirming reduced estrogen exposure during development. As adults, ATD-treated animals showed higher corticosterone responses at 30 min of restraint exposure compared with control animals as well as higher c-fos expression levels in the paraventricular nucleus of the hypothalamus. ATD treatment also increased stress-induced c-fos within several limbic regions of the forebrain, in addition to areas involved in somatosensory processing. Based on these results, we propose that the conversion of testosterone to estrogen during the neonatal period exerts marked, system-wide effects to organize adult neuroendocrine responses to homeostatic threat.
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Mirza, Zeenat, and Umama A. Abdel-dayem. "Uncovering Potential Roles of Differentially Expressed Genes, Upstream Regulators, and Canonical Pathways in Endometriosis Using an In Silico Genomics Approach." Diagnostics 10, no. 6 (June 19, 2020): 416. http://dx.doi.org/10.3390/diagnostics10060416.
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Endometriosis is characterized by ectopic endometrial tissue implantation, mostly within the peritoneum, and affects women in their reproductive age. Studies have been done to clarify its etiology, but the precise molecular mechanisms and pathophysiology remain unclear. We downloaded genome-wide mRNA expression and clinicopathological data of endometriosis patients and controls from NCBI’s Gene Expression Omnibus, after a systematic search of multiple independent studies comprising 156 endometriosis patients and 118 controls to identify causative genes, risk factors, and potential diagnostic/therapeutic biomarkers. Comprehensive gene expression meta-analysis, pathway analysis, and gene ontology analysis was done using a bioinformatics-based approach. We identified 1590 unique differentially expressed genes (129 upregulated and 1461 downregulated) mapped by IPA as biologically relevant. The top upregulated genes were FOS, EGR1, ZFP36, JUNB, APOD, CST1, GPX3, and PER1, and the top downregulated ones were DIO2, CPM, OLFM4, PALLD, BAG5, TOP2A, PKP4, CDC20B, and SNTN. The most perturbed canonical pathways were mitotic roles of Polo-like kinase, role of Checkpoint kinase proteins in cell cycle checkpoint control, and ATM signaling. Protein–protein interaction analysis showed a strong network association among FOS, EGR1, ZFP36, and JUNB. These findings provide a thorough understanding of the molecular mechanism of endometriosis, identified biomarkers, and represent a step towards the future development of novel diagnostic and therapeutic options.
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Adetiba, Emmanuel, Folarin Joseph Olaloye, Abdultaofeek Abayomi, Nasir Faruk, Sibusiso Moyo, Obiseye Obiyemi, and Surendra Thakur. "Compact automatic modulation recognition using over-the-air signals and FOS features." Bulletin of Electrical Engineering and Informatics 11, no. 4 (August 1, 2022): 2013–24. http://dx.doi.org/10.11591/eei.v11i4.4119.
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The recent deployment of automatic modulation recognition (AMR) for cognitive radio (CR) systems has significantly enhanced spectrum sensing capabilities. The utilization of real-time over-the-air digital radio frequency (RF) data for the development of a digital spectrum sensing model based on the automatic modulation classification (AMC) is presented in this study as a step for incorporating opportunistic spectrum sensing onto the NomadicBTS architecture. Some digital modulation techniques were studied for second-generation (2G) through fourth-generation (4G) technology. The raw RF signal dataset was digitized and curated, while non-complex first-order statistical (FOS) features were used with algorithms based on the Scaled conjugate gradient (SCG) and Levenberg-Marquardt (LM) to find the best learning algorithm for the generated AMR model. The results show that the developed AMR model has a very high likelihood of correctly classifying signals, with distinct patterns for each of the features of FOS. The results are compared to reveal a least mean square error (MSE) of 0.0131 with a maximum accuracy of 93.5 percent when the model was trained with seventy (70) neurons in the hidden layer using the LM method. The best model's accuracy will allow for the most precise identification of spectrum holes in the bands under consideration.
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Zhang, X. K., K. N. Wills, M. Husmann, T. Hermann, and M. Pfahl. "Novel pathway for thyroid hormone receptor action through interaction with jun and fos oncogene activities." Molecular and Cellular Biology 11, no. 12 (December 1991): 6016–25. http://dx.doi.org/10.1128/mcb.11.12.6016-6025.1991.
Full textAbstract:
Many essential biological pathways, including cell growth, development, and metabolism, are regulated by thyroid hormones (THs). TH action is mediated by intracellular receptors that belong to a large family of ligand-dependent transcription factors, including the steroid hormone and retinoic acid receptors. So far it has been assumed that TH receptors (TRs) regulate gene transcription only through the classical protein-DNA interaction mechanism. Here we provide evidence for a regulatory pathway that allows cross-talk between TRs and the signal transduction pathway used by many growth factors, oncogenes, and tumor promoters. In transient transfection studies, we observed that the oncogenes c-jun and c-fos inhibit TR activities, while TRs inhibit induction of the c-fos promoter and repress AP-1 site-dependent gene activation. A truncated TR that lacks only 17 amino acids from the carboxy terminus can no longer antagonize AP-1 activity. The cross-regulation between TRs and the signal transduction pathway appears to be based on the ability of TRs to inhibit DNA binding of the transcription factor AP-1 in the presence of THs. The constituents of AP-1, c-Jun, and c-Fos, vice versa, can inhibit TR-induced gene activation in vivo, and c-Jun inhibits TR DNA binding in vitro. This novel regulatory pathway is likely to play a major role in growth control and differentiation by THs.
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Zhang, X. K., K. N. Wills, M. Husmann, T. Hermann, and M. Pfahl. "Novel pathway for thyroid hormone receptor action through interaction with jun and fos oncogene activities." Molecular and Cellular Biology 11, no. 12 (December 1991): 6016–25. http://dx.doi.org/10.1128/mcb.11.12.6016.
Full textAbstract:
Many essential biological pathways, including cell growth, development, and metabolism, are regulated by thyroid hormones (THs). TH action is mediated by intracellular receptors that belong to a large family of ligand-dependent transcription factors, including the steroid hormone and retinoic acid receptors. So far it has been assumed that TH receptors (TRs) regulate gene transcription only through the classical protein-DNA interaction mechanism. Here we provide evidence for a regulatory pathway that allows cross-talk between TRs and the signal transduction pathway used by many growth factors, oncogenes, and tumor promoters. In transient transfection studies, we observed that the oncogenes c-jun and c-fos inhibit TR activities, while TRs inhibit induction of the c-fos promoter and repress AP-1 site-dependent gene activation. A truncated TR that lacks only 17 amino acids from the carboxy terminus can no longer antagonize AP-1 activity. The cross-regulation between TRs and the signal transduction pathway appears to be based on the ability of TRs to inhibit DNA binding of the transcription factor AP-1 in the presence of THs. The constituents of AP-1, c-Jun, and c-Fos, vice versa, can inhibit TR-induced gene activation in vivo, and c-Jun inhibits TR DNA binding in vitro. This novel regulatory pathway is likely to play a major role in growth control and differentiation by THs.
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Eremina, Olga Yurevna, Veronika Valentinovna Olifer, and Tatyana Alekseevna Davlianidze. "Prospects for the use of baits based on modern active ingredients against house flies." Disinfection affairs, no. 1 (March 2022): 33–37. http://dx.doi.org/10.35411/2076-457x-2022-1-33-37.
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Topical application revealed the high resistance of field populations of houseflies to pyrethroids (500–900?), neonicotinoids (345–500?), phenylpyrazoles (46–75?), susceptibility to FOS (0.6–1.4?) and oxadiazines (0.2?). With intestinal exposure, resistance rates are somewhat lower, but resistance remains at a high level to fipronil (33–76?), thiamethoxam (80–105?) and clothianidin (80–98?). Susceptibility to indoxacarb (0.2–1.4?) was revealed. The comparative efficacy of two modern baits based on indoxacarb and dinotefuran for resistant and susceptible strains of house flies was studied. Both insecticides are highly toxic to house flies in the form of commercially available baits. Indoxacarb and dinotefuran can be recommended for introduction into rotation schemes of insecticides in the form of food baits to control adult flies. Keywords: dinotefuran, indoxacarb, house flies, insecticide resistance, baits.
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Skinner, M., S. Qu, C. Moore, and R. Wisdom. "Transcriptional activation and transformation by FosB protein require phosphorylation of the carboxyl-terminal activation domain." Molecular and Cellular Biology 17, no. 5 (May 1997): 2372–80. http://dx.doi.org/10.1128/mcb.17.5.2372.
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The transcription factor AP-1, composed of Fos-Jun dimers, mediates some aspects of the cellular response to growth factors. Transcriptional activation and neoplastic transformation by FosB, a member of the Fos family of proteins, require the presence of a potent C-terminal activation domain. Here we show by mutational analysis that the FosB C-terminal domain has a proline-based motif that is essential for both of these functions. Phosphopeptide mapping experiments show that the C terminus of FosB is phosphorylated within a cluster of functionally redundant serine residues that is adjacent to this proline-based motif. Mutation of these serine residues to alanine severely reduces the ability of this region to function as an activation domain and inhibits the ability of FosB protein to function as a transforming protein. Several observations suggest that the kinase responsible for phosphorylation of these sites is distinct from the mitogen-activation protein kinases and stress-activated protein kinases. Our results show that transcriptional activation and neoplastic transformation by the FosB protein are dependent on phosphorylation within the C terminus. This form of control may provide a potential mechanism of signal integration at the level of a single transcription factor.
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Chen, Ting-Yun, Xiaoyun Li, Gillian C. Goobie, Ching-Hsia Hung, Tin-Kan Hung, Kyle Hamilton, Harinath Bahudhanapati, Jiangning Tan, Daniel J. Kass, and Yingze Zhang. "Identification of a distal RXFP1 gene enhancer with differential activity in fibrotic lung fibroblasts involving AP-1." PLOS ONE 16, no. 12 (December 31, 2021): e0254466. http://dx.doi.org/10.1371/journal.pone.0254466.
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Relaxin/insulin-like family peptide receptor 1 (RXFP1) mediates relaxin’s antifibrotic effects and has reduced expression in the lung and skin of patients with fibrotic interstitial lung disease (fILD) including idiopathic pulmonary fibrosis (IPF) and systemic sclerosis (SSc). This may explain the failure of relaxin-based anti-fibrotic treatments in SSc, but the regulatory mechanisms controlling RXFP1 expression remain largely unknown. This study aimed to identify regulatory elements of RXFP1 that may function differentially in fibrotic fibroblasts. We identified and evaluated a distal regulatory region of RXFP1 in lung fibroblasts using a luciferase reporter system. Using serial deletions, an enhancer upregulating pGL3-promoter activity was localized to the distal region between -584 to -242bp from the distal transcription start site (TSS). This enhancer exhibited reduced activity in IPF and SSc lung fibroblasts. Bioinformatic analysis identified two clusters of activator protein 1 (AP-1) transcription factor binding sites within the enhancer. Site-directed mutagenesis of the binding sites confirmed that only one cluster reduced activity (-358 to -353 relative to distal TSS). Co-expression of FOS in lung fibroblasts further increased enhancer activity. In vitro complex formation with a labeled probe spanning the functional AP-1 site using nuclear proteins isolated from lung fibroblasts confirmed a specific DNA/protein complex formation. Application of antibodies against JUN and FOS resulted in the complex alteration, while antibodies to JUNB and FOSL1 did not. Analysis of AP-1 binding in 5 pairs of control and IPF lung fibroblasts detected positive binding more frequently in control fibroblasts. Expression of JUN and FOS was reduced and correlated positively with RXFP1 expression in IPF lungs. In conclusion, we identified a distal enhancer of RXFP1 with differential activity in fibrotic lung fibroblasts involving AP-1 transcription factors. Our study provides insight into RXFP1 downregulation in fILD and may support efforts to reevaluate relaxin-based therapeutics alongside upregulation of RXFP1 transcription.
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Lee, B. A., and D. J. Donoghue. "Membrane-anchored form of v-sis/PDGF-B induces mitogenesis without detectable PDGF receptor autophosphorylation." Journal of Cell Biology 113, no. 2 (April 15, 1991): 361–70. http://dx.doi.org/10.1083/jcb.113.2.361.
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The v-sis protein is structurally and functionally related to PDGF. Forms of the v-sis protein which are anchored to the cell membrane via the transmembrane domain of the vesicular stomatitis virus G protein have been previously described (Hannink, M., and D.J. Donoghue. 1986. J. Cell Biol. 103:2311-2322). Several of these fusion proteins were shown to interact productively with the PDGF receptor (PDGFR) based on their ability to transform NIH 3T3 cells. In this report, we further characterized one of these membrane-anchored v-sis proteins, designated v-sis239-G. The gene encoding v-sis239-G was placed under control of the Drosophila melanogaster hsp70 promotor and synthesis of this protein was shown to induce a mitogenic response in NIH 3T3 cells. Unexpectedly, v-sis239-G did not induce detectable autophosphorylation of the PDGFR, in contrast to a similarly expressed secreted form of the v-sis protein. Thus, it appears that a PDGFR-mediated mitogenic response may be dissociated from detectable receptor autophosphorylation. Furthermore, induced synthesis of v-sis239-G was shown to lead to c-fos expression even in the absence of detectable receptor autophosphorylation. Interestingly, a nonmitogenic membrane-anchored form of the v-sis protein, designated v-sis239-G338, also induced c-fos without receptor autophosphorylation. These results raise interesting questions regarding the roles of autophosphorylation and c-fos induction in PDGFR-mediated signal transduction and suggest the possibility of an autophosphorylation-independent signal transduction pathway.
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Mangerud, Gunn, Niall W. Paterson, and Jonathan Bujak. "Permian palynoevents in the circum-Arctic region." Atlantic Geology 57 (January 24, 2021): 057–69. http://dx.doi.org/10.4138/atlgeol.2021.004.
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Permian palynofloras are recorded around the present-day Arctic and are typically dominated by taeniate and non-taeniate pollen, with intervals of spore domination. The assemblages show close similarities around the Arctic. Based on the published record, we present a compilation of 23 last occurrences (LOs), first occurrences (FOs), and some abundance events. These are anticipated to have regional correlation potential. In general, the Permian palynofloras of the Arctic have not been extensively studied, and the resolution is low due to a general lack of independent age control.
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San Andres, Joice V., Dana van Sambeek, Sydney Kinstler, Melanie Trenhaile-Gannemann, Shana Winkel, Andres Schlageter-Tello, Phillip Miller, and Thomas E. Burkey. "PSVII-19 Potential biomarkers of intestinal injury and inflammation in nursery pigs fed prebiotics." Journal of Animal Science 97, Supplement_2 (July 2019): 220. http://dx.doi.org/10.1093/jas/skz122.388.
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Abstract Prebiotics may alter protein fermentation and reduce biogenic amines that cause intestinal injury and inflammation. Biochemical markers are important in assessing gut integrity and inflammation, especially in evaluating efficacy of interventions for intestinal health at weaning. The objective of this study was to determine the effects of weaning and dietary prebiotics on potential biomarkers of intestinal barrier integrity and immunity. Sixty-four pigs (21 d; BW 6 ± 0.5 kg) of mixed sex were used to test 4 dietary treatments with 4 pens per treatment and 4 pigs per pen. Pens were randomly allocated to 1 of the 4 diets following completely randomized design in a 2-phase 28-d feeding experiment. Dietary treatments were corn-soybean based diet (Control), Control + fructooligosaccharide (FOS), Control + chicory pulp (PC) and Control + sugar beet pulp + (SBP). Prebiotics were incorporated in the diet by partially replacing corn at rate of 0.05 and 0.1% in phase 1 and 2, respectively. Blood samples were collected from 2 pigs per treatment on d 0, 14 and 28 and analyzed for concentrations of tight junction protein 1 (TJP1), intestinal fatty acid binding protein (IFABP), and diamine oxidase (DAO). Dietary treatments had no effect on growth performance. The TJP1 concentration increased with time (P < 0.001). The DAO and IFABP concentrations decreased (P < 0.001) over time. Pigs fed FOS and PC had lower concentrations of DAO (P = 0.034) and IFABP (P = 0.043) than Control and SBP at d 14. Results show that TJP1, DAO, and IFABP serum concentrations vary over the weaning period which may be indicative of intestinal restitution in response to weaning stress. Moreover, inclusion of prebiotics in the diet may affect serum biomarkers which may support the inclusion of prebiotics to improve gut health in weaned pigs.
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Mangerud, Gunn, Gilda Lopas, and Jonathan Bujak. "Carboniferous palynoevents in the circum-Arctic region." Atlantic Geology 57 (January 24, 2021): 041–55. http://dx.doi.org/10.4138/atlgeol.2021.003.
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The Carboniferous of the present-day Arctic yields an abundant and diverse association of terrestrial palynomorphs, particularly from Mississippian successions. Relatively few sections have been studied. However, those published so far demonstrate considerable similarities in the palynofloras between Arctic regions. Based on the published record, we present a compilation of 31 last occurrences (LOs), first occurrences (FOs), and some abundance events that have correlation potential around the Arctic. The chronostratigraphic resolution is relatively low, and the lack of independent age control hampers exact age assignments. But for most of these successions, palynology is the only dating tool available.
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Chen, Chun-Yen, Chao-Nan Lin, Rey-Shyong Chern, Yu-Chuan Tsai, Yung-Hsien Chang, and Chi-Hsien Chien. "Neuronal Activity Stimulated by Liquid Substrates Injection at Zusanli (ST36) Acupoint: The Possible Mechanism ofAquapuncture." Evidence-Based Complementary and Alternative Medicine 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/627342.
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Aquapunctureis a modified acupuncture technique and it is generally accepted that it has a greater therapeutic effect than acupuncture because of the combination of the acupoint stimulation and the pharmacological effect of the drugs. However, to date, the mechanisms underlying the effects ofaquapunctureremain unclear. We hypothesized that both the change in the local spatial configuration and the substrate stimulation ofaquapuncturewould activate neuronal signaling. Thus, bee venom, normal saline, and vitamins B1 and B12 were injected into a Zusanli (ST36) acupoint as substrate ofaquapuncture, whereas a dry needle was inserted into ST36 as a control. Afteraquapuncture, activated neurons expressing Fos protein were mainly observed in the dorsal horn of the spinal cord in lumbar segments L3–5, with the distribution nearly identical among all groups. However, the bee venom injection induced significantly more Fos-expressing neurons than the other substrates. Based on these data, we suggest that changes in the spatial configuration of the acupoint activate neuronal signaling and that bee venom may further strengthen this neuronal activity. In conclusion, the mechanisms for the effects ofaquapunctureappear to be the spatial configuration changes occurring within the acupoint and the ability of injected substrates to stimulate neuronal activity.
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Zhou, Libo, and Tao Wu. "A Network Pharmacology-Based Study on Vital Pharmacological Pathways and Targets of Eucommiae Cortex Acting on Osteoporosis." BioMed Research International 2022 (March 31, 2022): 1–14. http://dx.doi.org/10.1155/2022/8510842.
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Background. Eucommiae Cortex is a Chinese herbal medicine with bone protective effects and treats osteoporosis. This study aimed to explore the pharmacological mechanisms of this complex mixture. Methods. The active compounds and disease targets involved in the study were obtained from publicly available websites and databases. Core target genes were identified by protein-protein interaction (PPI) network and topology analysis and mapped to critical components by a “component-target” regulatory network. Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analysis and Gene Ontology (GO)analysis were performed to analyze the biological processes of target genes. Moreover, we carried out molecular docking, cell experiments, and quantitative real-time PCR to propel the research forward. Results. Eucommiae Cortex contained 28 active ingredients and 85 potential targets for antiosteoporosis. PPI networks and topology analysis screened 17 core target genes. The therapeutic mechanism involves a series of biological reactions, including host-virus response, chemical stress, oxidative stress, and cell cycle control. The KEGG enrichment illustrated that the MAPK signaling pathway might play a significant role. The final experiment detected ten genes (EGF, AKT1, JUN, MAPK8, MAPK1, CASP3, FOS, VEGFA, EGFR, and MYC) and three compounds (quercetin, kaempferol, and beta- carotene) in the MAPK pathway. Firstly, the CCK-8 and ALP activity test results showed that three compounds could enhance the proliferation and differentiation of osteoblast-like MC3T3-E1 cells. Secondly, molecular docking confirmed the favorable binding potential. Subsequently, we observed that adding 1 ∗ 10 − 6 mol/L quercetin, 1 ∗ 10 − 5 mol/L kaempferol, and 1 ∗ 10 − 5 mol/L beta-carotene activated the ERK/JNK cascades and the heterodimer complex AP-1(Fos/Jun) in the MAPK pathway. Conclusion. MAPK pathway might provide an essential mechanism for the antiosteoporosis effect of Eucommiae Cortex. Eucommiae Cortex and its active ingredients have the potential to treat osteoporosis.
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Pilecka, Elżbieta, Jacek Stanisz, Robert Kaczmarczyk, and Andrzej Gruchot. "The Setting of Strength Parameters in Stability Analysis of Open-Pit Slope Using the Random Set Method in the Bełchatów Lignite Mine, Central Poland." Energies 14, no. 15 (July 29, 2021): 4609. http://dx.doi.org/10.3390/en14154609.
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The slopes of open-pit mines are often at risk of failure. To identify this hazard, stability analyses are performed. An important element of these stability analyses is the reliable selection of input parameter values for the calculations. This selection is difficult because the slopes of the open pit are disturbed by mining activities. In such conditions, rheological processes, intensified by weathering, develop in open-pit slopes. This study is aimed at setting the strength parameters for the stability analysis of open-pit slopes with a developed slide process, using the random set method. The study was performed on the example of the open pit of the Bełchatów lignite mine, central Poland. A four-stage methodology, according to the random set method, was proposed. The methodology covered the following: site investigation, sensitivity analyses, shear strength reduction (SSR) analyses using numerical calculations, and probability analyses of the factor of safety (FoS) calculation results. The setting of the input parameters took into account the peak and residual strength parameters for each lithological unit in the physical model of the open-pit slope. Samples for laboratory tests were taken from the cores of nine test boreholes. The sensitivity analysis included all peak and residual strength parameters for each lithological unit in the body. As a result of the sensitivity analysis, specific strength parameters were adopted that would have a great impact upon the results of the calculations. Selected sets of parameter values were then used for the FoS calculations. The resultant FoS values revealed the probable slide planes. The positions of the slide planes were consistent with the interpreted slide surfaces based on the control boreholes and terrain observations. Knowledge of the slide planes positions and the values of the strength parameters enabled the designing of a securing approach for this landslide, and the taking of preventive measures to reduce this risk.
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Marshall, John E. A., Gunn Mangerud, Manuel Bringué, and Jonathan Bujak. "Devonian palynoevents in the circum-Arctic region." Atlantic Geoscience 58 (December 21, 2022): 307–28. http://dx.doi.org/10.4138/atlgeo.2022.015.
Full textAbstract:
Devonian strata of the present-day Arctic contain abundant and diverse assemblages of terrestrial palynomorphs, particularly from Middle and Upper Devonian sections; few intervals of Lower Devonian and Silurian strata have been palynologically studied, and these demonstrate strong palynofloral similarities across the region. Based on published records, we present a compilation of six last occurrence events (LOs), 44 first occurrence events (FOs) and one acme event that have proven of value in correlation in the present-day Arctic. Although their chronostratigraphic resolution is relatively low, and a scarcity of independent age control hampers precise age assignments, palynology is the only biostratigraphic discipline available for confident chronostratigraphic correlation of most Arctic Devonian strata.
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Labbadi, Moussa, Sahbi Boubaker, Mohamed Djemai, Souad Kamel Mekni, and Abdelghani Bekrar. "Fixed-Time Fractional-Order Global Sliding Mode Control for Nonholonomic Mobile Robot Systems under External Disturbances." Fractal and Fractional 6, no. 4 (March 22, 2022): 177. http://dx.doi.org/10.3390/fractalfract6040177.
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The present study addresses the problem of fixed-time stabilization (FTS) of mobile robots (MRs). The study’s distinguishing aspects are that the system under examination is subjected to external disturbances, and the system states are pushed to zero in a finite time. This paper suggests new control techniques for chained-form nonholonomic systems (CFNS) subjected to disturbances. First, a switching fractional-order (FO) control approach is proposed for a first-order subsystem (FOS) of an MR under complex disturbances. Secondly, an FO generic global sliding mode control approach is designed for the second-order system (SOS) of the MR in the presence of disturbances. The suggested sliding manifold for the SOS of the MR guarantees global system stability and reduces the chattering problem during control operations. A conventional quadratic Lyapunov function (QLF) is used to converge to the origin in a finite time (FnT). Through this study, a stabilizer for an MR in the presence of disturbances based on an FO switching time-varying controller that can stabilize immeasurable states in a fixed time is proposed. Finally, three case simulations are provided to demonstrate the efficacy of the control strategy proposed in this work against external disturbances.
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Mangerud, Gunn, Niall W. Paterson, and Jonathan Bujak. "Triassic palynoevents in the circum-Arctic region." Atlantic Geology 57 (March 14, 2021): 071–101. http://dx.doi.org/10.4138/atlgeol.2021.005.
Full textAbstract:
Triassic successions of the present-day Arctic contain abundant and diverse assemblages of nonmarine palynomorphs that have provided important biostratigraphic information. Dinoflagellate cyst are biostratigraphically useful in marine intervals in the Upper Triassic. Based on published records, we present a compilation of 78 last occurrences (LOs), first occurrences (FOs), and some abundance events that are anticipated to have correlation potential in the Arctic region. Palynological work has been carried out in many Arctic areas, with extensive palynological research published on the Triassic successions of the Norwegian Barents Sea and Svalbard. An updated, recent palynological zonation scheme exists for that region, integrating previous schemes and illustrating the chronostratigraphic value of palynology in the Triassic. For the Lower and Middle Triassic, good ammonoid control ties the palynological zones to the chronostratigraphic scale. Independent control is sparse, and resolution is lower in the Upper Triassic, so that palynology is commonly the only biostratigraphic discipline available for chronostratigraphic dating and correlation.
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Dars, Sindhrani, Hayley Uden, Saravana Kumar, and Helen A. Banwell. "When, why and how foot orthoses (FOs) should be prescribed for children with flexible pes planus: a Delphi survey of podiatrists." PeerJ 6 (April 16, 2018): e4667. http://dx.doi.org/10.7717/peerj.4667.
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Background Flexible pes planus (flat feet) in children is a common reason parents and caregivers seek health professionals consult and a frequent reason podiatrists prescribe foot orthoses. Yet no universal agreement exists on the diagnosis of this condition, or when and how foot orthoses should be prescribed. The aim of this study was to garner consensus and agreement among podiatrists on the use of FOs for paediatric flexible pes planus. Methods A three round Delphi consensus survey was undertaken with 15 podiatry experts from Australia, New Zealand and the United Kingdom. Round One gathered consensus on the diagnosis and intervention into paediatric pes planus with specific questions on types of FOs and prescription variables used. Round Two and Three were based on answers from Round One and gathered agreement (rationale for choices) on a five point Likert scale. 70% of respondents had to agree to a statement for it to be accepted as consensus or agreement. Results Consensus and agreement was achieved for 83 statements directing the diagnosis of pes planus (using FPI-6 and/or rearfoot measures), common signs and symptoms (e.g., pain, fatigue, abnormal gait and other functional concerns) that direct when to intervene into paediatric flexible pes planus. Prefabricated orthoses were the preferred intervention where adequate control is gained with their use. When customised orthoses are prescribed, a vertical [heel] cast pour (71.4%) and minimal arch fill (76.9%) are the prescription variables of choice, plus or minus additional variables (i.e., medial heel (Kirby) skive, the use of a University of California Biomechanical Laboratory device or a medial flange) dependent on level of disorder and plane of excessive motion. Conclusions This study identified consensus and agreement on a series of diagnosis methods and interventions for the paediatric flexible pes planus. A clinical protocol was developed from the resultant consensus statements which provides clinicians with a series of evidenced-informed statements to better guide them on when, how and why FOs are used specific to this population.
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Comba, Rachel, Nicole Gervais, Dave Mumby, and Matthew Holahan. "Emergence of spatial behavioral function and associated mossy fiber connectivity and c-Fos labeling patterns in the hippocampus of rats." F1000Research 4 (July 27, 2015): 396. http://dx.doi.org/10.12688/f1000research.6822.1.
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Improvement on spatial tasks is observed during a late, postnatal developmental period (PND18 – PND24). The purpose of the current work was 1) to determine whether the emergence of spatial-behavioral function was based on the ability to generate appropriate behavioral output; 2) to assess whether mossy fiber connectivity patterns preceded the emergence of spatial-behavioral function; 3) to explore functional changes in the hippocampus to determine whether activity in hippocampal networks occurred in a training-dependent or developmentally-dependent fashion. To these ends, male, Long Evans rats were trained on a spatial water or dry maze task for one day (PND16, PND18 or PND20) then euthanized. Training on these 2 tasks with opposing behavioral demands (swimming versus exploration) was hypothesized to control for behavioral topology. Only at PND20 was there evidence of spatial-behavioral function for both tasks. Examination of synaptophysin staining in the CA3 region (i.e., mossy fiber projections) revealed enhanced connectivity patterns that preceded the emergence of spatial behavior. Analysis of c-Fos labeling (functional changes) revealed developmentally-dependent increases in c-Fos positive cells in the dentate gyrus, CA3 and CA1 regions whereas training-dependent increases were noted in the CA3 and CA1 regions for the water-maze trained groups. Results suggest that changes in mossy fiber connectivity in association with enhanced hippocampal functioning precede the emergence of spatial behavior observed at PND20. The combination of neuroanatomical and behavioural results confirms the hypothesis that this time represents a sensitive period for hippocampal development and modification and the emergence of spatial/ cognitive function.
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Dickerson, Sarah M., Stephanie L. Cunningham, Heather B. Patisaul, Michael J. Woller, and Andrea C. Gore. "Endocrine Disruption of Brain Sexual Differentiation by Developmental PCB Exposure." Endocrinology 152, no. 2 (December 29, 2010): 581–94. http://dx.doi.org/10.1210/en.2010-1103.
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Abstract In mammals, sexual differentiation of the hypothalamus occurs during prenatal and early postnatal development due in large part to sex differences in hormones. These early organizational processes are critically important for the attainment and maintenance of adult reproductive functions. We tested the hypothesis that perinatal exposure to polychlorinated biphenyls (PCBs) that disrupt hormonal pathways would perturb reproductive maturation and the sexually dimorphic development of neuroendocrine systems in the preoptic area (POA). Pregnant Sprague-Dawley rats were injected on gestational d 16 and 18 with vehicle (dimethylsulfoxide), Aroclor 1221 (A1221, an estrogenic PCB mix), a reconstituted PCB mixture representing those highest in human body burden (PCBs 138, 153, 180), or estradiol benzoate, an estrogenic control. Male and female pups were monitored for somatic and reproductive development. In adulthood, some rats were perfused and used for immunohistochemistry of estrogen receptor α, kisspeptin, and coexpression of Fos in GnRH neurons. Other rats were used to obtain fresh-frozen POA dissections for use in a PCR-based 48-gene expression array. Pubertal onset was advanced and estrous cyclicity irregular in endocrine-disrupted females. Furthermore, sexual differentiation of female neuroendocrine systems was masculinized/defeminized. Specifically, in the adult female anteroventral periventricular nucleus, estrogen receptor α-cell numbers and kisspeptin fiber density were significantly decreased, as was GnRH-Fos coexpression. PCR analysis identified androgen receptor, IGF-I, N-methyl-d-aspartate receptor subunit NR2b, and TGFβ1 mRNAs as significantly down-regulated in endocrine-disrupted female POAs. These data suggest that developmental PCBs profoundly impair the sexual differentiation of the female hypothalamus.
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Mittelman-Smith, Melinda A., Sally J. Krajewski-Hall, Nathaniel T. McMullen, and Naomi E. Rance. "Ablation of KNDy Neurons Results in Hypogonadotropic Hypogonadism and Amplifies the Steroid-Induced LH Surge in Female Rats." Endocrinology 157, no. 5 (March 3, 2016): 2015–27. http://dx.doi.org/10.1210/en.2015-1740.
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Abstract In the human infundibular (arcuate) nucleus, a subpopulation of neurons coexpress kisspeptin and neurokinin B (NKB), 2 peptides required for normal reproductive function. A homologous group of neurons exists in the arcuate nucleus of rodents, termed KNDy neurons based on the coexpression of kisspeptin, NKB, and dynorphin. To study their function, we recently developed a method to selectively ablate KNDy neurons using NK3-SAP, a neurokinin 3 receptor agonist conjugated to saporin (SAP). Here, we ablated KNDy neurons in female rats to determine whether these neurons are required for estrous cyclicity and the steroid induced LH surge. NK3-SAP or Blank-SAP (control) was microinjected into the arcuate nucleus using stereotaxic surgery. After monitoring vaginal smears for 3–4 weeks, rats were ovariectomized and given 17β-estradiol and progesterone in a regimen that induced an afternoon LH surge. Rats were killed at the time of peak LH levels, and brains were harvested for NKB and dual labeled GnRH/Fos immunohistochemistry. In ovary-intact rats, ablation of KNDy neurons resulted in hypogonadotropic hypogonadism, characterized by low levels of serum LH, constant diestrus, ovarian atrophy with increased follicular atresia, and uterine atrophy. Surprisingly, the 17β-estradiol and progesterone-induced LH surge was 3 times higher in KNDy-ablated rats. Despite the marked increase in the magnitude of the LH surge, the number of GnRH or anterior ventral periventricular nucleus neurons expressing Fos was not significantly different between groups. Our studies show that KNDy neurons are essential for tonic levels of serum LH and estrous cyclicity and may play a role in limiting the magnitude of the LH surge.
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Kahn, Julia B., Russell G. Port, Cuiyong Yue, Hajime Takano, and Douglas A. Coulter. "Circuit-based interventions in the dentate gyrus rescue epilepsy-associated cognitive dysfunction." Brain 142, no. 9 (July 30, 2019): 2705–21. http://dx.doi.org/10.1093/brain/awz209.
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Abstract Temporal lobe epilepsy is associated with significant structural pathology in the hippocampus. In the dentate gyrus, the summative effect of these pathologies is massive hyperexcitability in the granule cells, generating both increased seizure susceptibility and cognitive deficits. To date, therapeutic approaches have failed to improve the cognitive symptoms in fully developed, chronic epilepsy. As the dentate’s principal signalling population, the granule cells’ aggregate excitability has the potential to provide a mechanistically-independent downstream target. We examined whether normalizing epilepsy-associated granule cell hyperexcitability—without correcting the underlying structural circuit disruptions—would constitute an effective therapeutic approach for cognitive dysfunction. In the systemic pilocarpine mouse model of temporal lobe epilepsy, the epileptic dentate gyrus excessively recruits granule cells in behavioural contexts, not just during seizure events, and these mice fail to perform on a dentate-mediated spatial discrimination task. Acutely reducing dorsal granule cell hyperactivity in chronically epileptic mice via either of two distinct inhibitory chemogenetic receptors rescued behavioural performance such that they responded comparably to wild type mice. Furthermore, recreating granule cell hyperexcitability in control mice via excitatory chemogenetic receptors, without altering normal circuit anatomy, recapitulated spatial memory deficits observed in epileptic mice. However, making the granule cells overly quiescent in both epileptic and control mice again disrupted behavioural performance. These bidirectional manipulations reveal that there is a permissive excitability window for granule cells that is necessary to support successful behavioural performance. Chemogenetic effects were specific to the targeted dorsal hippocampus, as hippocampal-independent and ventral hippocampal-dependent behaviours remained unaffected. Fos expression demonstrated that chemogenetics can modulate granule cell recruitment via behaviourally relevant inputs. Rather than driving cell activity deterministically or spontaneously, chemogenetic intervention merely modulates the behaviourally permissive activity window in which the circuit operates. We conclude that restoring appropriate principal cell tuning via circuit-based therapies, irrespective of the mechanisms generating the disease-related hyperactivity, is a promising translational approach.
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Zhirnov, Andrey A., Konstantin V. Stepanov, Stanislav G. Sazonkin, Tatyana V. Choban, Kirill I. Koshelev, Anton O. Chernutsky, Alexey B. Pnev, Alexey O. Novikov, and Dmitriy A. Yagodnikov. "Study of Intra-Chamber Processes in Solid Rocket Motors by Fiber Optic Sensors." Sensors 21, no. 23 (November 25, 2021): 7836. http://dx.doi.org/10.3390/s21237836.
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In this study, an experimental study of the burning rate of solid fuel in a model solid propellant rocket motor (SRM) E-5-0 was conducted using a non-invasive control method with fiber-optic sensors (FOSs). Three sensors based on the Mach–Zehnder interferometer (MZI), fixed on the SRM E-5-0, recorded the vibration signal during the entire cycle of solid fuel burning. The results showed that, when using MZI sensors, the non-invasive control of solid fuel burnout is made possible both by recording the time of arrival of the combustion front to the sensor and by analyzing the peaks on the spectrogram of the recorded FOS signal. The main mode of acoustic vibrations of the chamber of the model SRM is longitudinal, and it changes with time, depending on the chamber length. Longitudinal modes of the combustion chamber were detected by MZI only after the combustion front passed its fixing point, and the microphone was unable to register them at all. The results showed that the combustion rate was practically constant after the first second, which was confirmed by the graph of the pressure versus time at the nozzle exit.
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Kiatwanidvilai, Somyot, and Rawinun Praserttaweelap. "Neurofuzzy c-Means Network-Based SCARA Robot for Head Gimbal Assembly (HGA) Circuit Inspection." Computational Intelligence and Neuroscience 2018 (December 2, 2018): 1–10. http://dx.doi.org/10.1155/2018/4952389.
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Decision and control of SCARA robot in HGA (head gimbal assembly) inspection line is a very challenge issue in hard disk drive (HDD) manufacturing. The HGA circuit called slider FOS is a part of HDD which is used for reading and writing data inside the disk with a very small dimension, i.e., 45 × 64 µm. Accuracy plays an important role in this inspection, and classification of defects is very crucial to assign the action of the SCARA robot. The robot can move the inspected parts into the corresponding boxes, which are divided into 5 groups and those are “Good,” “Bridging,” “Missing,” “Burn,” and “No connection.” A general image processing technique, blob analysis, in conjunction with neurofuzzy c-means (NFC) clustering with branch and bound (BNB) technique to find the best structure in all possible candidates was proposed to increase the performance of the entire robotics system. The results from two clustering techniques which are K-means, Kohonen network, and neurofuzzy c-means were investigated to show the effectiveness of the proposed algorithm. Training results from the 30x microscope inspection with 300 samples show that the best accuracy for clustering is 99.67% achieved from the NFC clustering with the following features: area, moment of inertia, and perimeter, and the testing results show 92.21% accuracy for the conventional Kohonen network. The results exhibit the improvement on the clustering when the neural network was applied. This application is one of the progresses in neurorobotics in industrial applications. This system has been implemented successfully in the HDD production line at Seagate Technology (Thailand) Co. Ltd.
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Pogenberg, Vivian, Larissa Consani-Textor, and Matthias Wilmanns. "DNA binding and dimerization specificities of Basic Zipper transcription factors." Acta Crystallographica Section A Foundations and Advances 70, a1 (August 5, 2014): C1404. http://dx.doi.org/10.1107/s2053273314085957.
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The ability of basic zipper transcription factors to form homo- or heterodimers provides a paradigm for combinatorial control of eukaryotic gene expression. In a first study, we clarified the specificity of the MIcrophthalmia-associated Transcription Factor [1]. To achieve this, we solved three crystal structures: two structures of MITF in complex with DNA duplexes encompassing two different target motifs (E-box and M-box) and one APO-structure. We then analyzed interactions between these DNA elements and several MITF mutants with documented mice phenotypes, using complementary techniques. The comparison of these experiments together with available biological data reveals the particular mechanism of DNA recognition by MITF. Moreover we demonstrated how a shift in the leucine zipper register limits the choice of the homotypic dimerization partner among the other b-HLH-Zip transcription factors. In a second study, we wondered how facultative dimerization results in alternative DNA-binding repertoires on distinct regulatory elements [2]. In this respect, the hematopoietic b-Zip transcription factor MafB, is a good model, since it has the ability to form homo- and heterodimers with a few other transcription factors. We first determined two high-resolution structures of MafB as a homodimer and as a heterodimer with c-Fos bound to variants of the Maf-recognition element (MARE). The two structures revealed several unexpected and specific coiled coil interactions. Based on these findings, we have engineered two MafB mutants with opposite dimerization preferences. One of them indeed showed a strong preference for MafB/c-Fos heterodimerization. In addition this variant enabled a selection of heterodimer- favoring over homodimer-specific MARE variants, demonstrating that protein/protein and protein/DNA interactions are interconnected. Our data provide a new concept for transcription factor design to selectively activate dimer-specific pathways and binding repertoires.
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Fatoumata BA, Soli KONE, Samba SOUMARE, Jens Bernt AUNE, Urbain DEMBELE, Siaka D. TRAORE, and Kalifa TRAORE. "ANALYSIS OF PRODUCERS' ADAPTATION STRATEGIES TO THE CHALLENGES OF CLIMATE CHANGE IN FLOOD CONTROL PRODUCTION SYSTEMS IN YÉLIMANÉ, KAYES REGION." International Journal of Agriculture, Environment and Bioresearch 07, no. 06 (2022): 193–208. http://dx.doi.org/10.35410/ijaeb.2022.5784.
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Our study aims to contribute to improving the state of knowledge of the adaptation strategies of producers in flood recession production systems in Yélimané. The methodology is based on the collection of data at the level of the commune, where a general assembly made it possible to choose the villages, to define and validate criteria for classifying households into wealthy, moderately wealthy and poor on the basis of the level of equipment and the differentiation of flood recession production systems. A questionnaire was administered to heads of households, resource persons and a focus group interview guide with FOs and other actors involved in the field of Agriculture. In terms of results, 330 households were surveyed, including 110 per category. 65% are men and 35% women. 91% are married and 70% are illiterate. The average household size is 34.2, 27.5 and 17.4 people; they have an average area of 14.3, 4.8 and 5.2 ha respectively for types A, B and C. Poorly equipped, they practice flood recession and rainfed crops. The data shows that almost all households (96%) have actually observed climate change and the consequences are felt in the drop in yields and income. Also, households are limited to the types and diversity of crops they can grow. Among them, 59% of households have adopted strategies to reduce these negative effects and make ad hoc and technical adjustments in anticipation for all seasons.
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Meggendorfer, Manja, Wencke Walter, Stephan Hutter, Wolfgang Kern, Claudia Haferlach, and Torsten Haferlach. "FOS Expression Distinguishes Two Groups of Atypical CML (aCML) Allowing Targeted Therapy." Blood 132, Supplement 1 (November 29, 2018): 3893. http://dx.doi.org/10.1182/blood-2018-99-111832.
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Abstract BCR-ABL1 negative myeloproliferative neoplasms not only include atypical chronic myeloid leukemia (aCML), but also chronic myelomonocytic leukemia (CMML), chronic neutrophilic leukemia (CNL), and myelodysplastic/myeloproliferative neoplasm, unclassifiable (MDS/MPN, U). Despite the recent advances in characterizing aCML more specifically, based on next generation sequencing data, the differential diagnosis and subsequent treatment decisions remain difficult. Therefore, we analyzed the transcriptome and performed whole genome sequencing (WGS) in a cohort of morphologically defined 231 patients (pts): 49 aCML, 30 CNL, 50 MDS/MPN, U, and 102 CMML all diagnosed according to WHO classification. WGS libraries were prepared with the TruSeq PCR free library prep kit and sequenced on a NovaSeq 6000 or HiSeqX instrument with 100x coverage (Illumina, San Diego, CA). The Illumina tumor/unmatched normal workflow was used for variant calling. To remove potential germline variants, each variant was queried against the gnomAD database, variants with global population frequencies >1% where excluded. For transcriptome analysis total RNA was sequenced on the NovaSeq 6000 with a median of 50 mio. reads per sample. The obtained estimated gene counts were normalized and the resulting log2 counts per million (CPMs) were used as a proxy of gene expression. Unsupervised exploratory analysis techniques, such as principal component analysis (PCA) and hierarchical clustering (HC) were used to identify groups of samples with similar expression profiles. We observed a high similarity between the different entities with CMML being the most distant entity, followed by CNL. MDS/MPN, U and aCML were the most similar entities. Due to high within-group heterogeneity, we found that it was impossible to identify a gene expression signature that separated aCMLs reliably from the other MDS/MPN overlap entities. Surprisingly, PCA as well as HC indicated the existence of two subgroups within the aCMLs. Therefore, we searched for genes with a bimodal-like expression profile. We found that FOS expression levels strongly separated aCMLs into two groups of 16 pts (FOSlow) and 33 pts (FOShi), respectively. Interestingly, FOShi correlated with mutations in SETBP1 (12/33, 36% vs. 3/16, 19%), a known marker typically mutated in aCML (Figure 1a). Addressing the mutational landscape of these two groups (FOShivs.FOSlow) we found that ASXL1 (88% vs. 100%), TET2 (33% vs. 50%), SRSF2 (45% vs. 56%), EZH2 (27% vs. 31%), and NRAS (21% vs. 25%) showed rather similar mutation frequencies. GATA2 (15% vs. 31%) and RUNX1 (18% vs. 38%) mutations were less frequent in FOShi, whereas SETBP1 and CBL (18% vs. 6%) were more frequent in this group. Consistent with known features of SETBP1 mutation this group showed a higher white blood cell count (78 x109/L vs. 52 x109/L) and platelet count (158x109/L vs. 90x109/L), although none of these differences were significant. The two groups were further analyzed for gene expression differences and we found 16 genes with synchronized upregulation within the FOShi group that were differentially expressed (FDR < 0.05, absolute logFC > 1.5) compared to FOSlow. Functional enrichment analysis linked those genes with regulation of cell proliferation (p<0.001), negative regulation of cell death (p<0.001), and the AP-1 complex (p<0.001). Those 16 genes included the transcription factors JUN, FOSB, EGR3, and KLF4, the cancer-related genes DUSP1, RHOB, OSM, TNFRSF10C, and CXCR2, and the FDA approved drug targets JUN, COX-2, and FCGR3B (Figure 1b). JUN/FOS are the main components of the AP-1 complex, a regulator of cell life and death. The upregulation of these genes results in increased proliferation as clinically observed in aCML pts. Furthermore, for these pts a treatment with INFα might result in an anti-proliferative effect by modulation of FOS transcript levels. Further, COX-2 inhibitors might also suppress proliferation and differentiation of leukemia cells. However, for the FOSlow pts these treatments might not be as effective due to the already low expression levels of the respective genes. Since the expression levels of FOShi equal those of MDS/MPN overlap, whereas FOSlow levels are closer to the ones of a healthy control cohort, SETBP1 mutation might be a marker and indicator for pts with high FOS expression and therefore providing further treatment options by targeting specifically the FOS mediated pathways. Disclosures Meggendorfer: MLL Munich Leukemia Laboratory: Employment. Walter:MLL Munich Leukemia Laboratory: Employment. Hutter:MLL Munich Leukemia Laboratory: Employment. Kern:MLL Munich Leukemia Laboratory: Employment, Equity Ownership. Haferlach:MLL Munich Leukemia Laboratory: Employment, Equity Ownership. Haferlach:MLL Munich Leukemia Laboratory: Employment, Equity Ownership.
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Nurmawati, Thatit, Yeni Kartika Sari, and Fina Ayu Ningtyas. "Antidiabetes Serbuk Umbi Tanaman Yakon (<i>Smallanthus sonchifolius</i>) pada Kadar Gula Darah Tikus Putih (<i>Rattus norvegicus</i>)." BIOSAINTROPIS (BIOSCIENCE-TROPIC) 7, no. 1 (August 31, 2021): 56–62. http://dx.doi.org/10.33474/e-jbst.v7i1.393.
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The routine and long-term use of oral antidiabetic drugs in DM patients can make the public's perception worried about the side effects of the drug. In the Yakon (SS) plant tuber there is Fructooligosacaraide (FOS) which can reduce blood sugar levels. The research objective was to determine the effect of Yakon plant tubers on blood sugar levels in white rats with type 2 diabetes mellitus model. This type of research was experimental with a pre-post test design with control-group design. Independent variable, administration of Yakon plant tuber powder, dependent variable, blood glucose levels. The sample was divided into 2 groups (dose 1, dose 2) and a control group, each of 6 rats. The mice were given a high carbohydrate diet for 9 weeks. Treatment dose 1 (100 mg / kgBB) for 3 days. Measurement of blood sugar levels using Gluco-Dr. Data analysis used Paired Sample T-test, Independent Sample T-test, and One Way Anova. The results showed that the rats in the treatment group dose 1 experienced a decrease in the average blood sugar level of 122.83 mg / dl (p 0.002) (p 0.009). This shows that there is an effect of using Yakon plant tuber powder on blood sugar levels. The results of this study were the control group and the treatment group dose 1 (Sig. (2-tailed) = 0.000, α = 0.05). Meanwhile, the comparison results showed that there was a difference between the control group and the treatment group at dose 1, Based on the above research, Yakon tubers can be used to reduce blood sugar levels.
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Łukowicz, Krzysztof, Barbara Zagrajczuk, Karolina Truchan, Łukasz Niedzwiedzki, Katarzyna Cholewa-Kowalska, and Anna M. Osyczka. "Chemical Compounds Released from Specific Osteoinductive Bioactive Materials Stimulate Human Bone Marrow Mesenchymal Stem Cell Migration." International Journal of Molecular Sciences 23, no. 5 (February 26, 2022): 2598. http://dx.doi.org/10.3390/ijms23052598.
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In this work, a poly(L-lactide-co-glycolide) (PLGA)-based composite was enriched with one of the following sol-gel bioactive glasses (SBG) at 50 wt.%: A1—40 mol% SiO2, 60 mol% CaO, CaO/SiO2 ratio of 1.50; S1—80 mol% SiO2, 20 mol% CaO, CaO/SiO2 ratio of 0.25; A2—40 mol% SiO2, 54 mol% CaO, 6 mol% P2O5, CaO/SiO2 ratio of 1.35; S2—80 mol% SiO2,16 mol% CaO, 4 mol% P2O5, CaO/SiO2 ratio of 0.20. The composites and PLGA control sheets were then soaked for 24 h in culture media, and the obtained condition media (CM) were used to treat human bone marrow stromal cells (hBMSCs) for 72 h. All CMs from the composites increased ERK 1/2 activity vs. the control PLGA CM. However, expressions of cell migration-related c-Fos, osteopontin, matrix metalloproteinase-2, C-X-C chemokine receptor type 4, vascular endothelial growth factor, and bone morphogenetic protein 2 were significantly increased only in cells treated with the CM from the A1/PLGA composite. This CM also significantly increased the rate of human BMSC migration but did not affect cell metabolic activity. These results indicate important biological markers that are upregulated by products released from the bioactive composites of a specific chemical composition, which may eventually prompt osteoprogenitor cells to colonize the bioactive material and accelerate the process of tissue regeneration.
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Wang, Shi-jin, Li-feng Xi, and Bing-hai Zhou. "FBS-enhanced agent-based dynamic scheduling in FMS." Engineering Applications of Artificial Intelligence 21, no. 4 (June 2008): 644–57. http://dx.doi.org/10.1016/j.engappai.2007.05.012.
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