Dissertations / Theses on the topic 'Forensic analysis'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Forensic analysis.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
Fairbanks, Kevin D. "Forensic framework for honeypot analysis." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/33977.
Full textRodríguez, Mariela, and James Winefordner. "Elemental Forensic Analysis of Glass." Revista de Química, 2007. http://repositorio.pucp.edu.pe/index/handle/123456789/99672.
Full textAt identifying glass as forensic evidence, there is a continued move away from dependence on physical properties measured, such as index of refraction and density, towards methods of elemental analysis of its traces. Several spectroscopic, radiochemical and mass spectrometry techniques have been applied for multielemental analysis of small samples of glass. This review shows some of the most commonly used methods, as well as their advantages and limitations.
Gettings, Katherine Butler. "Forensic Ancestry and Phenotype SNP Analysis and Integration with Established Forensic Markers." Thesis, The George Washington University, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3590467.
Full textWhen an evidential DNA profile does not match identified suspects or profiles from available databases, further DNA analyses targeted at inferring the possible ancestral origin and phenotypic characteristics of the perpetrator could yield valuable information. Single Nucleotide Polymorphisms (SNPs), the most common form of genetic polymorphisms, have alleles associated with specific populations and/or correlated to physical characteristics. With this research, single base primer extension (SBE) technology was used to develop a 50 SNP assay designed to predict ancestry among the primary U.S. populations (African American, East Asian, European, and Hispanic/Native American), as well as pigmentation phenotype. The assay has been optimized to a sensitivity level comparable to current forensic DNA analyses, and has shown robust performance on forensic-type samples. In addition, three prediction models were developed and evaluated for ancestry in the U.S. population, and two models were compared for eye color prediction, with the best models and interpretation guidelines yielding correct information for 98% and 100% of samples, respectively. Also, because data from additional DNA markers (STR, mitochondrial and/or Y chromosome DNA) may be available for a forensic evidence sample, the possibility of including this data in the ancestry prediction was evaluated, resulting in an improved prediction with the inclusion of STR data and decreased performance when including mitochondrial or Y chromosome data. Lastly, the possibility of using next-generation sequencing (NGS) to genotype forensic STRs (and thus, the possibility of a multimarker multiplex incorporating all forensic markers) was evaluated on a new platform, with results showing the technology incapable of meeting the needs of the forensic community at this time.
Nilsson, Martina. "Mitochondrial DNA in Sensitive Forensic Analysis." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7458.
Full textShah, Jayna J. "Microfluidic devices for forensic DNA analysis." Fairfax, VA : George Mason University, 2007. http://hdl.handle.net/1920/2878.
Full textTitle from PDF t.p. (viewed Jan. 22, 2008). Thesis director: Rao V. Mulpuri. Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Electrical and Computer Engineering. Vita: p. 159. Includes bibliographical references (p. 145-158). Also available in print.
Ogwu, John. "Forensic pharmaceutical analysis of counterfeit medicines." Thesis, De Montfort University, 2018. http://hdl.handle.net/2086/17552.
Full textvan, den Berg Jelle, and Filip Lagerholm. "Forensic Analysis of the Nintendo Switch." Thesis, Högskolan i Halmstad, Akademin för informationsteknologi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-42451.
Full textLu, Andy. "Forensic analysis on wireless medical devices." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2022. https://ro.ecu.edu.au/theses/2541.
Full textEide, Jarle, and Jan Ove Skogheim Olsen. "Forensic analysis of an unknown embedded device." Thesis, Norges Teknisk-Naturvitenskaplige Universitet, Institutt for datateknikk og informasjonsvitenskap, 2006. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-10060.
Full textEvery year thousands of new digital consumer device models come on the market. These devices include video cameras, photo cameras, computers, mobile phones and a multitude of different combinations. Most of these devices have the ability to store information in one form or another. This is a problem for law enforcement agencies as they need access to all these new kinds of devices and the information on them in investigations. Forensic analysis of electronic and digital equipment has become much more complex lately because of the sheer number of new devices and their increasing internal technological sophistication. This thesis tries to help the situation by reverse engineering a Qtek S110 device. More specifically we analyze how the storage system of this device, called the object store, is implemented on the devices operating system, Windows Mobile. We hope to figure out how the device stores user data and what happens to this data when it is "deleted". We further try to define a generalized methodology for such forensic analysis of unknown digital devices. The methodology takes into account that such analysis will have to be performed by teams of reverse-engineers more than single individuals. Based on prior external research we constructed and tested the methodology successfully. We were able to figure our more or less entirely the object stores internal workings and constructed a software tool called BlobExtractor that can extract data, including "deleted", from the device without using the operating system API. The main reverse engineering strategies utilized was black box testing and disassembly. We believe our results can be the basis for future advanced recovery tools for Windows Mobile devices and that our generalized reverse engineering methodology can be utilized on many kinds of unknown digital devices.
Edlund, Hanna. "Sensitive Identification Tools in Forensic DNA Analysis." Doctoral thesis, Uppsala universitet, Institutionen för genetik och patologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-131904.
Full textGreening, Christopher. "Automatic writer identification for forensic document analysis." Thesis, University of Essex, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.520166.
Full textMcCallum, Nicola Ann. "Analysis of RNA stability in forensic specimens." Thesis, University of Strathclyde, 2015. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=24829.
Full textThakur, Neha S. "Forensic Analysis of WhatsApp on Android Smartphones." ScholarWorks@UNO, 2013. http://scholarworks.uno.edu/td/1706.
Full textSwortwood, Madeleine Jean. "Comprehensive Forensic Toxicological Analysis of Designer Drugs." FIU Digital Commons, 2013. http://digitalcommons.fiu.edu/etd/997.
Full textVan, Winkle Carolyn. "Forensic DNA Extraction Strategies for PCR Analysis." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc278269/.
Full textMcCulley, Shane. "Forensic Analysis of G Suite Collaborative Protocols." ScholarWorks@UNO, 2017. http://scholarworks.uno.edu/td/2386.
Full textButtigieg, Gavin. "Forensic and toxicological applications of atomic analysis." Diss., The University of Arizona, 2003. http://hdl.handle.net/10150/280402.
Full textSusaimanickam, Rojesh. "A workflow to support forensic database analysis." Thesis, Susaimanickam, Rojesh (2012) A workflow to support forensic database analysis. Masters by Research thesis, Murdoch University, 2012. https://researchrepository.murdoch.edu.au/id/eprint/13741/.
Full textChow, W. M. L. "Capilliary column gas chromatography in forensic science." Thesis, University of Strathclyde, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371945.
Full textShakir, Amer, Muhammad Hammad, and Muhammad Kamran. "Comparative Analysis & Study of Android/iOS MobileForensics Tools." Thesis, Högskolan i Halmstad, Akademin för informationsteknologi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-44797.
Full textCai, Yue. "Nondestructive multi-element analysis of colorants for forensic applications and artwork authentication." HKBU Institutional Repository, 2013. https://repository.hkbu.edu.hk/etd_ra/1528.
Full textByrne, Ana M. "Forensic anthropology : its contribution to forensic cases submitted to the University of Montana for analysis." The University of Montana, 2006. http://etd.lib.umt.edu/theses/available/etd-07202006-142543/.
Full textWalnoha, Michael Anthony. "Shoeprint analysis a GIS application in forensic evidence /." Morgantown, W. Va. : [West Virginia University Libraries], 2006. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=4639.
Full textTitle from document title page. Document formatted into pages; contains iv, 64 p. : ill. (some col.), maps (some col.). Includes abstract. Includes bibliographical references (p. 63-64).
Baize, John. "Forensic Soil Analysis: Characterization of the Sand Fraction." TopSCHOLAR®, 1997. http://digitalcommons.wku.edu/theses/767.
Full textJohansson, Christian. "Computer Forensic Text Analysis with Open Source Software." Thesis, Blekinge Tekniska Högskola, Institutionen för programvaruteknik och datavetenskap, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-4994.
Full textHales, Gavin. "Assisting digital forensic analysis via exploratory information visualisation." Thesis, Abertay University, 2016. https://rke.abertay.ac.uk/en/studentTheses/774128b9-957e-4a05-aa74-dbeefebb8113.
Full textSoobhany, Ahmad Ryad. "Image source identification and characterisation for forensic analysis." Thesis, Keele University, 2013. http://eprints.keele.ac.uk/2301/.
Full textDivne, Anna-Maria. "Evaluation of New Technologies for Forensic DNA Analysis." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5744.
Full textWest, M. J. "Applications of Raman Microscopy to Trace Forensic Analysis." Thesis, University of Kent, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.520885.
Full textCroft, Debra Jane. "Forensic geoscience : development of techniques for soil analysis." Thesis, Royal Holloway, University of London, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406177.
Full textTse, Wai-hin Kenneth, and 謝維軒. "Forensic analysis using FAT32 file cluster allocation patterns." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46605733.
Full textReidy, Lisa Jayne. "Stable isotope analysis : a new forensic science tool." Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.479310.
Full textNelson, Alexander J. "Software signature derivation from sequential digital forensic analysis." Thesis, University of California, Santa Cruz, 2016. http://pqdtopen.proquest.com/#viewpdf?dispub=10140317.
Full textHierarchical storage system namespaces are notorious for their immense size, which is a significant hindrance for any computer inspection. File systems for computers start with tens of thousands of files, and the Registries of Windows computers start with hundreds of thousands of cells. An analysis of a storage system, whether for digital forensics or locating old data, depends on being able to reduce the namespaces down to the features of interest. Typically, having such large volumes to analyze is seen as a challenge to identifying relevant content. However, if the origins of files can be identified—particularly dividing between software and human origins—large counts of files become a boon to profiling how a computer has been used. It becomes possible to identify software that has influenced the computer's state, which gives an important overview of storage system contents not available to date.
In this work, I apply document search to observed changes in a class of forensic artifact, cell names of the Windows Registry, to identify effects of software on storage systems. Using the search model, a system's Registry becomes a query for matching software signatures. To derive signatures, file system differential analysis is extended from between two storage system states to many sequences of states. The workflow that creates these signatures is an example of analytics on data lineage, from branching data histories. The signatures independently indicate past presence or usage of software, based on consistent creation of measurably distinct artifacts. A signature search engine is demonstrated against a machine with a selected set of applications installed and executed. The optimal search engine according to that machine is then turned against a separate corpus of machines with a set of present applications identified by several non-Registry forensic artifact sources, including the file systems, memory, and network captures. The signature search engine corroborates those findings, using only the Windows Registry.
Senthivel, Saranyan. "Automatic Forensic Analysis of PCCC Network Traffic Log." ScholarWorks@UNO, 2017. http://scholarworks.uno.edu/td/2394.
Full textSwaminathan, Ashwin. "Multimedia forensic analysis via intrinsic and extrinsic fingerprints." College Park, Md.: University of Maryland, 2008. http://hdl.handle.net/1903/8776.
Full textThesis research directed by: Dept. of Electrical and Computer Engineering. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
Rinke, Caitlin. "Selective Multivariate Applications in Forensic Science." Doctoral diss., University of Central Florida, 2012. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/5459.
Full textPh.D.
Doctorate
Chemistry
Sciences
Chemistry
Tagesson, Samuel. "Anti-forensik mot minnesforensik : En litteraturstudie om anti-forensiska metoder mot minnesdumpning och minnesanalys." Thesis, Högskolan i Skövde, Institutionen för informationsteknologi, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17818.
Full textIT forensics face many difficulties in their work of obtaining and analyzing data. Criminals are using more and more anti-forensic methods to hide evidence that can be used against them. One common anti-forensic method is encryption. In order for IT forensics to access the encrypted information, the encryption key can be found in the memory of the computer. This makes the computer's memory valuable to retrieved and analyze. However, there are several anti-forensic methods that a criminal can use to prevent the memory from being retrieved or analyzed. This study performs a systematic literature study to identify the current anti-forensic methods against memory analysis and memory dumping on Windows system. Several methods are addressed where, among other things, the operating system is modified or built-in security functions on the CPU are used to prevent information being retrieved or analyzed from memory.
Schultz, John S. "Offline forensic analysis of Microsoft Windows XP physical memory." Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2006. http://library.nps.navy.mil/uhtbin/hyperion/06Sep%5FSchultz.pdf.
Full textThesis Advisor(s): Chris Eagle. "September 2006." Includes bibliographical references (p. 73-74). Also available in print.
Dowler, Shaun Wallace. "Applications of hyperspectral imaging techniques to forensic image analysis." Thesis, University of Auckland, 2010. http://hdl.handle.net/2292/9604.
Full textHaak, Daniel James. "Analysis of The University of Montana Forensic Case 29." The University of Montana, 2010. http://etd.lib.umt.edu/theses/available/etd-06032010-145520/.
Full textPeisert, Sean Philip. "A model of forensic analysis using goal-oriented logging." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2007. http://wwwlib.umi.com/cr/ucsd/fullcit?p3246091.
Full textTitle from first page of PDF file (viewed March 9, 2007). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 156-165).
Carpenter, Robert Christopher. "Inductively coupled plasma-optical emission spectrometry for forensic analysis." Thesis, University of Plymouth, 1985. http://hdl.handle.net/10026.1/2680.
Full textYu, Holly April. "Novel Approaches to Forensic Explosives Recovery, Storage and Analysis." Thesis, Curtin University, 2017. http://hdl.handle.net/20.500.11937/57348.
Full textChurinsky, Candace Renee. "Characterization of carbon electrode surfaces development of biosensors for forensic DNA applications." Thesis, Boston University, 2013. https://hdl.handle.net/2144/21139.
Full textQuantitative polymerase chain reaction (qPCR) techniques are currently used to quantify samples containing deoxyribonucleic acid (DNA) in forensic analyses. This technology can provide valuable information to an analyst regarding the amount of DNA present but lacks the ability to determine the quality of the sample. Electrochemistry-based biosensors that utilize screen-printed electrodes may provide a method to determine the number of DNA molecules and the length of those molecules in a single assay. This work aimed to create a biosensor by electrostatically loading TPOX oligonucleotides onto a carbon screen-printed electrode for the purpose of quantifying genomic DNA. Electrochemical signal was obtained via the indicating molecule bis-benzimide H33258, which preferentially interacts with double-stranded DNA and would indicate a hybridization event. Cyclic voltammetry was chosen to measure the current signal; peaks obtained using this technique can be analyzed with the Randles-Sevčik equation, which relates current signal with concentration of the target species. A large amount of signal variation and background charging current was observed when H33258 was used as the redox probe. This led to a study of the surface characteristics of the carbon electrodes themselves (i.e. effective surface area) by utilizing the reversible and well-characterized redox couple hexaammine ruthenium. The effect of electrode activation at high anodic potentials was also studied. Though highly recommended in the literature, activation of the carbon surface caused effective surface area and charging current to increase. While a larger electro-active surface is often desirable, the high background current generated when activation is used within the protocol can mask the signal of interest. Due to the low signal-to-noise ratio and inability to reuse the carbon electrode, it was concluded that carbon screen printed electrodes are not optimal forensic DNA biosensors.
2031-01-01
Wanogho, S. O. "The forensic analysis of soils with particular reference to particle size distribution analysis." Thesis, University of Strathclyde, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381113.
Full textHashim, Noor Hayati. "An architecture for the forensic analysis of Windows system generated artefacts." Thesis, University of South Wales, 2011. https://pure.southwales.ac.uk/en/studentthesis/forensic-analysis-of-windows-system-generated-artefacts(be571569-2afe-4d52-8c99-9dbc8388b1db).html.
Full textGustafsson, Kevin, and Emil Sundstedt. "Automated file extraction in a cloud environment for forensic analysis." Thesis, Blekinge Tekniska Högskola, Institutionen för datalogi och datorsystemteknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-14692.
Full textMöjligheten att använda OpenStacks ögonblicks funktion som metod för att säkra bevis har granskats i detta papper. Dessutom har möjligheten att extrahera bevis automatiskt med ett befintligt automatiseringsverktyg undersökts. Användbarheten av ögonblicksbilder i en rättslig utredning undersöktes genom att genomföra en serie tester påbåde ögonblicksbilder och fysiska disk avbilder. Resultaten av testerna jämfördes sedan för att utvärdera användbarheten av ögonblicksbilden. Automatisk utvinning av bevis undersöktes genom att implementera en lösning med Ansible och utvärdera algoritmen baserat påden befintliga standarden ISO 27037. Det drogs slutsatsen att de ögonblicksbilder som skapats av OpenStack beter sig tillräckligt lika en fysisk disk för att avbilderna ska vara användbara vid en råttslig utredning. Den algoritm som föreslås att extrahera bevis automatiskt tycks inte bryta mot standarden.
Connon, Catherine Cupples. "Improving Processing Efficiency for Forensic DNA Samples." Thesis, University of North Texas, 2015. https://digital.library.unt.edu/ark:/67531/metadc799515/.
Full textMachado, Marcos Paulo Salles 1974. "Análise retrospectiva dos exames realizados no serviço de antropologia forense do Instituto Médico Legal Afrânio Peixoto, Rio de Janeiro-Brasil = Retrospective analysis of the skills performed in the forensic anthropology service of the Afrânio Peixoto Medical Legal Institute, Rio de Janeiro-Brasil." [s.n.], 2015. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290733.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Made available in DSpace on 2018-08-27T18:33:28Z (GMT). No. of bitstreams: 1 Machado_MarcosPauloSalles_M.pdf: 1542270 bytes, checksum: 2ded2442ee636b63adfcabdc553824aa (MD5) Previous issue date: 2015
Resumo: O objetivo deste trabalho foi realizar a análise retrospectiva dos casos investigados nos dois primeiros anos de funcionamento do Serviço de Antropologia Forense (SAFO) do Rio de Janeiro. Foram recebidas 66 requisições de exames expedidas por autoridades policiais ou judiciárias e cada requisição deu origem a um laudo. Seis requisições continham ossos de origem não humana, três representavam materiais sem interesse forense, enquanto uma outra requisição solicitava exame complementar. As demais 56 requisições continham ossos de 74 diferentes indivíduos, pois nove requisições encaminhavam ossos de mais de uma pessoa. Cinco das 74 ossadas não deram entrada no laboratório do SAFO por terem sido identificados pelo Serviço de Odontologia Forense ou pelo Setor de Necropapiloscopia. Logo, 69 ossadas foram periciadas e o resultados desses exames compõe o presente estudo. Dentre as 56 requisições, 47 (83%) eram constituídas de remanescentes de um único indivíduo, enquanto 9 (17%) traziam ossos de mais de uma pessoa. Menos de 18,8% das 69 ossadas tiveram mais de 95% dos ossos do corpo recuperados, enquanto que apenas 10,14% das 47 ossadas encontradas completamente esqueletizadas tiveram mais de 50% dos seus ossos recuperados. O perfil biológico resultante dos exames das ossadas revelou que a amostra era predominantemente constituída por homens (80%), caucasoides (32%), adultos-jovens entre 21-50 anos (54%) constituindo o grupo de risco. O trauma mais frequentemente observado foi o contundente, correspondendo a 33,3% dos casos, seguido do perfurocontundente, observado em 31,4%. A região mais atingida foi a crânio-cervical, atingida em 42% dos traumas. A relação mais alta observada entre um tipo de trauma e uma região específica do corpo foi a ação perfurocontundente incidindo sobre a região crânio-cervical, correspondendo à 27,3%. 52,1% das ações perfurocontundentes foram observadas na região crânio-cervical. Por fim, a analise dos locais de encontro de ossadas evidenciou um grande número de encontro reduzido a poucas áreas da cidade, revelando que existe concentração da violência
Abstract: The aim of this study was to perform a retrospective analysis of the cases investigated in the first two years of the Forensic Anthropology Service (SAFO) of Rio de Janeiro. Over these two years the laboratory received 66 examination requisitions requested by police or judicial authorities and each of these requests has resulted in a report. Six corresponded to non-human material, other 3 represented material without forensic implications and another one corresponded to a complementary examination requisition. The remaining 56 requisitions comprised a total of 74 different individuals, once there were 9 cases with bones of more than one person. Five out of 74 skeletal remains did not enter the SAFO laboratory because they had been positively identified by the Forensic Dentistry Office or through Necropapiloscopy. Therefore 69 skeletal remains were examined and the resulting data compose the present study. Out of 56 requisitions, 47 (83%) presented skeletal remains of single individuals; whereas the other 9 (17%) conteined bones of more than one individual. Less than 18.8% of 69 skeletal remains recovered comprised more than 95% of body bones, wile only 10.14% of the 47 fully skeletonized remains had more than half of the bones recovered. The biological profile established after skeletal remains were investigated showed that the sample was made up mainly of male subjects (80%), caucasian (32%), young-adults ranging from ages 21-50 (54%), considered as the risk group. Injuries most frequently observed were blunt trauma (33.3%), followed by gunshot (31.4%). The most susceptible body regions were the head and neck, affected in 42% of cases. The highest correlation between type of trauma to a specific body part was observed with gunshot injuries to the head-neck region, corresponding to 27.3% of the cases. Gunshot trauma represented 52.1% of injuries found on the head and neck. Analysis of the places where the remains were recovered reveled a great number of cases restricted to a few areas of the city, which shows the violence is concentrated in these localities
Mestrado
Odontologia Legal e Deontologia
Mestre em Biologia Buco-Dental
Tau, Tiroyamodimo. "A forensic analysis of genetic variation in the Botswana population." University of the Western cape, 2016. http://hdl.handle.net/11394/5657.
Full textThis thesis has been placed under a long term embargo. Forensic and population genetic parameters were investigated in the Botswana population using autosomal and Y-chromosome short tandem repeat markers. AmpFlSTR Profiler plus markers were used to investigate the genetic diversity and forensic parameters in 773 individuals from Botswana from the reference database of the Botswana Police. The levels of polymorphism found using the AmpFlSTR Profiler Plus markers showed that the nine loci that make up the AmpFlSTR Profiler Plus can differentiate individuals for forensic casework in the Botswana population. AmpFlSTR Identifiler autosomal STR markers were used to investigate the population structure according to ethno-linguistics and geography 990 individuals from Botswana that serve as a reference database for the Botswana Police. Using pairwise genetic distances (Fst), analysis of molecular variance (AMOVA), factorial correspondence analysis (FCA), and the unsupervised Bayesian clustering method found in STRUCTURE and the landscape genetics software TESS, ethno-linguistics were found to have a greater influence on population structure than geography. The patterns of population structure found using these markers highlight the need for regional reference databases that include both ethnolinguistic and geographic location information. These markers have important potential for bio-anthropological studies as well as for forensic applications. The 17 Y-chromosomal short tandem repeats found in AmpFlSTR Y-filer and a highly discriminatory Y-STR genotyping system (the Y-STR 10-plex developed in the Forensics DNA Laboratory at the University of the Western Cape) were analysed in 249 unrelated male individuals from Botswana. Rst, multi-dimensional scaling (MDS) and AMOVA were used to investigate population differentiation in Botswana. The discrimination capacity (DC) was found to be higher using the Y-STR 10-plex as compared to the 17 markers in the Y-filer genotyping system. No geographic regional or ethnic differentiation was observed between the Northern and Southern regions of Botswana using both marker systems. Regional and ethnic variation can be useful in forensic working hypotheses. Cluster analysis using the highly discriminatory Y-STR 10-plex haplotypes may provide information about ancestry and haplogroup information.
National Research Foundation (NRF)