Relevant bibliographies by topics / Fly-derived DNA

Academic literature on the topic 'Fly-derived DNA'

Author: Grafiati
Published: 10 December 2022
Last updated: 29 January 2023

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Journal articles on the topic "Fly-derived DNA"

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Schubert, Grit, Melanie Stockhausen, Constanze Hoffmann, Kevin Merkel, Linda Vigilant, Fabian H. Leendertz, and Sébastien Calvignac-Spencer. "Targeted detection of mammalian species using carrion fly-derived DNA." Molecular Ecology Resources 15, no. 2 (August 4, 2014): 285–94. http://dx.doi.org/10.1111/1755-0998.12306.

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Gogarten, Jan F., Constanze Hoffmann, Mimi Arandjelovic, Andreas Sachse, Kevin Merkel, Paula Dieguez, Anthony Agbor, et al. "Fly‐derived DNA and camera traps are complementary tools for assessing mammalian biodiversity." Environmental DNA 2, no. 1 (October 29, 2019): 63–76. http://dx.doi.org/10.1002/edn3.46.

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Calvignac‐Spencer, Sébastien, Kevin Merkel, Nadine Kutzner, Hjalmar Kühl, Christophe Boesch, Peter M. Kappeler, Sonja Metzger, Grit Schubert, and Fabian H. Leendertz. "Carrion fly‐derived DNA as a tool for comprehensive and cost‐effective assessment of mammalian biodiversity." Molecular Ecology 22, no. 4 (January 8, 2013): 915–24. http://dx.doi.org/10.1111/mec.12183.

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Kamakaka, R. T., P. D. Kaufman, B. Stillman, P. G. Mitsis, and J. T. Kadonaga. "Simian virus 40 origin- and T-antigen-dependent DNA replication with Drosophila factors in vitro." Molecular and Cellular Biology 14, no. 8 (August 1994): 5114–22. http://dx.doi.org/10.1128/mcb.14.8.5114-5122.1994.

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DNA replication of double-stranded simian virus 40 (SV40) origin-containing plasmids, which has been previously thought to be a species-specific process that occurs only with factors derived from primate cells, is catalyzed with an extract derived from embryos of the fruit fly Drosophila melanogaster. This reaction is dependent upon both large T antigen, the SV40-encoded replication initiator protein and DNA helicase, and a functional T-antigen binding site at the origin of DNA replication. The efficiency of replication with extracts derived from Drosophila embryos is approximately 10% of that observed with extracts prepared from human 293 cells. This activity is not a unique property of embryonic extracts, as cytoplasmic extracts from Drosophila tissue culture cells also support T-antigen-mediated replication of SV40 DNA. By using highly purified proteins, DNA synthesis is initiated by Drosophila polymerase alpha-primase in a T-antigen-dependent manner in the presence of Drosophila replication protein A (RP-A; also known as single-stranded DNA-binding protein), but neither human RP-A nor Escherichia coli single-stranded DNA-binding protein could substitute for Drosophila RP-A. In reciprocal experiments, however, Drosophila RP-A was able to substitute for human RP-A in reactions carried out with human polymerase alpha-primase. These results collectively indicate that many of the specific functional interactions among T antigen, polymerase alpha-primase, and RP-A are conserved from primates to Drosophila species. Moreover, the observation that SV40 DNA replication can be performed with Drosophila factors provides a useful assay for the study of bidirectional DNA replication in Drosophila species in the context of a complete replication reaction.
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Kamakaka, R. T., P. D. Kaufman, B. Stillman, P. G. Mitsis, and J. T. Kadonaga. "Simian virus 40 origin- and T-antigen-dependent DNA replication with Drosophila factors in vitro." Molecular and Cellular Biology 14, no. 8 (August 1994): 5114–22. http://dx.doi.org/10.1128/mcb.14.8.5114.

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DNA replication of double-stranded simian virus 40 (SV40) origin-containing plasmids, which has been previously thought to be a species-specific process that occurs only with factors derived from primate cells, is catalyzed with an extract derived from embryos of the fruit fly Drosophila melanogaster. This reaction is dependent upon both large T antigen, the SV40-encoded replication initiator protein and DNA helicase, and a functional T-antigen binding site at the origin of DNA replication. The efficiency of replication with extracts derived from Drosophila embryos is approximately 10% of that observed with extracts prepared from human 293 cells. This activity is not a unique property of embryonic extracts, as cytoplasmic extracts from Drosophila tissue culture cells also support T-antigen-mediated replication of SV40 DNA. By using highly purified proteins, DNA synthesis is initiated by Drosophila polymerase alpha-primase in a T-antigen-dependent manner in the presence of Drosophila replication protein A (RP-A; also known as single-stranded DNA-binding protein), but neither human RP-A nor Escherichia coli single-stranded DNA-binding protein could substitute for Drosophila RP-A. In reciprocal experiments, however, Drosophila RP-A was able to substitute for human RP-A in reactions carried out with human polymerase alpha-primase. These results collectively indicate that many of the specific functional interactions among T antigen, polymerase alpha-primase, and RP-A are conserved from primates to Drosophila species. Moreover, the observation that SV40 DNA replication can be performed with Drosophila factors provides a useful assay for the study of bidirectional DNA replication in Drosophila species in the context of a complete replication reaction.
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Rodgers, Torrey W., Charles C. Y. Xu, Jacalyn Giacalone, Karen M. Kapheim, Kristin Saltonstall, Marta Vargas, Douglas W. Yu, Panu Somervuo, W. Owen McMillan, and Patrick A. Jansen. "Carrion fly-derived DNA metabarcoding is an effective tool for mammal surveys: Evidence from a known tropical mammal community." Molecular Ecology Resources 17, no. 6 (August 19, 2017): e133-e145. http://dx.doi.org/10.1111/1755-0998.12701.

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Abbasi, Ibrahim, Artur Trancoso Lopo de Queiroz, Oscar David Kirstein, Abdelmajeed Nasereddin, Ben Zion Horwitz, Asrat Hailu, Ikram Salah, et al. "Plant-feeding phlebotomine sand flies, vectors of leishmaniasis, preferCannabis sativa." Proceedings of the National Academy of Sciences 115, no. 46 (October 29, 2018): 11790–95. http://dx.doi.org/10.1073/pnas.1810435115.

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Blood-sucking phlebotomine sand flies (Diptera: Psychodidae) transmit leishmaniasis as well as arboviral diseases and bartonellosis. Sand fly females become infected withLeishmaniaparasites and transmit them while imbibing vertebrates’ blood, required as a source of protein for maturation of eggs. In addition, both females and males consume plant-derived sugar meals as a source of energy. Plant meals may comprise sugary solutions such as nectar or honeydew (secreted by plant-sucking homopteran insects), as well as phloem sap that sand flies obtain by piercing leaves and stems with their needle-like mouthparts. Hence, the structure of plant communities can influence the distribution and epidemiology of leishmaniasis. We designed a next-generation sequencing (NGS)–based assay for determining the source of sand fly plant meals, based upon the chloroplast DNA gene ribulose bisphosphate carboxylase large chain (rbcL). Here, we report on the predilection of several sand fly species, vectors of leishmaniasis in different parts of the world, for feeding onCannabis sativa. We infer this preference based on the substantial percentage of sand flies that had fed onC. sativaplants despite the apparent “absence” of these plants from most of the field sites. We discuss the conceivable implications of the affinity of sand flies forC. sativaon their vectorial capacity forLeishmaniaand the putative exploitation of their attraction toC. sativafor the control of sand fly-borne diseases.
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Haymer, David S., Donald O. Mcinnis, and Loretta Arcangeli. "Genetic variation between strains of the Mediterranean fruit fly, Ceratitis capitata, detected by DNA fingerprinting." Genome 35, no. 3 (June 1, 1992): 528–33. http://dx.doi.org/10.1139/g92-077.

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DNA fingerprinting has been used to detect genetic variation in the Mediterranean fruit fly, Ceratitis capitata. Three different probes have been identified that can be used to detect DNA restriction fragment length polymorphisms between strains of this species. The strains used in this study differ only in terms of their geographic origin or genetic background. One of the probes used is the bacteriophage vector M13, and the other two are repetitive sequences derived from the medfly genome based on a weak homology to M13. Within a strain, each probe produces a consistent restriction fragment profile that is not affected by the method or timing of DNA extraction. Between strains, when M13 is used as a probe, an average of 10% of the observable bands are polymorphic. Use of the medfly genomic sequences as a probe increases the proportion of polymorphic bands between strains up to 30%. The fact that genetic differences between even such closely related strains can be reliably detected by this method holds great promise for studies of insect pests including the ability to monitor the movements of pest species, determining the extent of genetic variation in pest populations, and in making identifications from otherwise unidentifiable material.Key words: genetic variation, Ceratitis capitata, DNA fingerprinting.
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Lee, J. H., S. M. Kaeppler, R. A. Graybosch, and R. G. Sears. "A PCR assay for detection of a 2RL.2BS wheat–rye chromosome translocation." Genome 39, no. 3 (June 1, 1996): 605–8. http://dx.doi.org/10.1139/g96-076.

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A 2RL.2BS wheat–rye translocation, present in the wheat germplasm line Hamlet, carries a gene for resistance to Hessian fly biotype L, one of the most virulent biotypes presently encountered in wheat production environments. Unlike several other wheat–rye chromosome translocations common in wheat breeding programs, 2RL lacks genes encoding storage proteins or other easily selected markers. Oligonucleotide primers synthesized from published sequences derived from the R173 family of moderately repetitive rye DNA were used in the DNA polymerase chain reaction (PCR) to identify specific markers for 2RL. The same primers, when used with DNA extracted from additional wheat–rye translocation lines of importance to the wheat breeding community, gave distinctive PCR products for each genotype. The single primer pair, PAWS5 and PAWS6, may, therefore, have wide applicability for the identification of wheat–rye chromosomal translocations presently encountered in wheat breeding populations. Key words : 2RL.2BS wheat–rye chromosome translocation, polymerase chain reaction, detection.
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Duncan, G. A., P. H. Adler, K. P. Pruess, and T. O. Powers. "Molecular differentiation of two sibling species of the black fly Simulium vittatum (Diptera: Simuliidae) based on random amplified polymorphic DNA." Genome 47, no. 2 (April 1, 2004): 373–79. http://dx.doi.org/10.1139/g03-144.

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Larvae of the black fly morphospecies Simulium vittatum from Colorado, Montana, Nebraska, and New Hampshire were cytologically identified as either the IS-7 or the IIIL-1 cytospecies. DNA was PCR amplified from cytotyped larvae using eight 10-mer primers, labeled with 33P, and run on polyacrylamide gels. The entire data set of 96 amplicons produced incomplete separation of the two cytospecies when subjected to neighbor-joining and maximum parsimony analyses. However, when analyzed within geographical regions, separate species status was supported. Bootstrap support for distinctness of the two cytospecies was best in Colorado where they were collected in true sympatry. The IS-7 cytospecies was more polymorphic in the western states, where it differed most from IIIL-1, which was most variable in the eastern states. The frequencies of the 17 most common amplicons in the two cytospecies were inversely correlated. A model of speciation derived from the molecular evidence suggests that IS-7 evolved in the west and spread eastward, whereas IIIL-1 later originated in the east and spread westward.Key words: Simulium vittatum, cytospecies, RAPD analysis, molecular markers, population structure.
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Book chapters on the topic "Fly-derived DNA"

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Pei, Lei, and Markus Schmidt. "Containment strategies for synthetic gene drive organisms and impacts on gene flow." In Gene flow: monitoring, modeling and mitigation, 137–52. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789247480.0010.

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Abstract Gene drives, particularly synthetic gene drives, may help to address some important challenges, by efficiently altering specific sections of DNA in entire populations of wild organisms. Here we review the current development of the synthetic gene drives, especially those RNA-guided synthetic gene drives based on the CRISPR nuclease Cas. Particular focuses are on their possible applications in agriculture (e.g. disease resistance, weed control management), ecosystem conservation (e.g. evasion species control), health (e.g. to combat insect-borne and fungal infections), and for basic research in model organisms (e.g. Saccharomyces, fruit fly, and zebra fish). The physical, chemical, biological, and ecological containment strategies that might help to confine these gene drive-modified organisms are then explored. The gene flow issues, those from gene drive-derived organisms to the environment, are discussed, while possible mitigation strategies for gene drive research are explored. Last but not least, the regulatory context and opinions from key stakeholders (regulators, scientists, and concerned organizations) are reviewed, aiming to provide a more comprehensive overview of the field.
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Yee, David P., and Tim Hunkapiller. "Overview: A System for Tracking and Managing the Results from Sequence Comparison Programs." In Pattern Discovery in Biomolecular Data. Oxford University Press, 1999. http://dx.doi.org/10.1093/oso/9780195119404.003.0017.

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The Human Genome Project was launched in the early 1990s to map, sequence, and study the function of genomes derived from humans and a number of model organisms such as mouse, rat, fruit fly, worm, yeast, and Escherichia coli. This ambitious project was made possible by advances in high-speed DNA sequencing technology (Hunkapiller et al., 1991). To date, the Human Genome Project and other large-scale sequencing projects have been enormously successful. The complete genomes of several microbes (such as Hemophilus influenzae Rd, Mycoplasma genitalium, and Methanococcus jannaschii) have been completely sequenced. The genome of bacteriophage T4 is complete, and the 4.6-megabase sequence of E. coli and the 13-megabase genome of Saccharomyces cerevisiae have just recently also been completed. There are 71 megabases of the nematode Caenorhabditis elegans available. Six megabases of mouse and 60 megabases of human genomic sequence have been finished, which represent 0.2% and 2% of their respective genomes. Finally, more than 1 million expressed sequence tags derived from human and mouse complementary DNA expression libraries are publicly available. These public data, in addition to private and proprietary DNA sequence databases, represent an enormous information-processing challenge and data-mining opportunity. The need for common interfaces and query languages to access heterogeneous sequence databases is well documented, and several good systems are well underway to provide those interfaces (Woodsmall and Benson, 1993; Marr, 1996). Our own work on database and program interoperability in this domain and in computational chemistry (Gushing, 1995) has shown, however, that providing the interface is but the first step toward making these databases fully useful to the researcher. (Here, the term “database” means a collection of data in electronic form, which may not necessarily be physically deposited in a database management system [DBMS]. A scientist’s database could thus be a collection of flat files, where the term “database” means “data stored in a DBMS” is clear from the context.) Deciphering the genomes of sequenced organisms falls into the realm of analysis; there is now plenty of sequence data. The most common form of sequence analysis involves the identification of homologous relationships among similar sequences.
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