Relevant bibliographies by topics / Fluorescent Nanoparticle

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Academic literature on the topic 'Fluorescent Nanoparticle'

Author: Grafiati
Published: 7 September 2023

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Journal articles on the topic "Fluorescent Nanoparticle"

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Nurgaziyeva, Elmira, Sarkyt Kudaibergenov, Grigoriy Mun, and Vitaliy Khutoryanskiy. "Synthesis of fluorescently-labelled poly(2-ethyl-2-oxazoline)-protected gold nanoparticles." Chemical Bulletin of Kazakh National University, no. 1 (March 19, 2021): 12–20. http://dx.doi.org/10.15328/cb1185.

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Gold nanoparticles (GNPs) protected by poly(2-ethyl-2-oxazoline) (POZ) of different molecular weights (Mw = 5, 50, 200 and 500 kDa) were synthesised and characterised by dynamic light scattering, nanoparticle tracking analysis, zeta potential measurement and transmission electron microscopy. It was established that the use of POZ with 50 kDa resulted in formation of GNPs with low polydispersity while POZ with greater molecular weights led to formation of more polydisperse GNPs. Fluorescent labelling of these nanoparticles was achieved through their reaction with polyethyleneglycol dithiol (8-12 kDa) as a linker molecule with subsequent reaction with 6-(iodoacetamido) fluorescein. The fluorescent nature of obtained GNPs was confirmed by the appearance of the fluorescence peak at 510 nm that is typical for fluorescein molecules and glowing of the aqueous solution under the UV irradiaton. The fluorescently-labelled GNPs are promising tool in biomedical application to monitor the biological systems using fluorescent microscopy.
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Sasaki, Isabelle, Jonathan Daniel, Sébastien Marais, Jean-Baptiste Verlhac, Michel Vaultier, and Mireille Blanchard-Desce. "Soft fluorescent organic nanodots as nanocarriers for porphyrins." Journal of Porphyrins and Phthalocyanines 23, no. 11n12 (December 2019): 1463–69. http://dx.doi.org/10.1142/s108842461950158x.

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Novel fluorescent organic nanoparticles made from citric acid and diethylenetriamine were used as biocompatible and highly water-soluble nanocarriers for hydrophobic tetraphenylporphyrin (TPP). The tetraphenylporphyrin units were covalently attached to the nanoparticles, generating conjugated nanoparticles which retain water solubility and preserve the photophysical properties of monomeric TPP. The conjugated nanoparticles show two distinct fluorescence features: blue emission from the nanoparticle when excited in the near-UV (360 nm) and characteristic far-red emission of the TPP when excited in the visible (Soret band or Q bands). The uptake of the conjugated nanoparticles in live human neuroblastoma cancer cells was evidenced using two-photon microscopy. These experiments demonstrate that the fluorescent organic nanoparticles do act as efficient nanocarriers, allowing cell internalization of hydrophobic porphyrins. These conjugated nanoparticles appear as promising nanotools for theranostic (based on the combination of imaging and monitoring of the nanoparticle fluorescence) and therapeutic (photodynamic therapy by selectively exciting the grafted porphyrin units) modalities.
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Dalavi, Dattatray K., Avinash Kamble, Dhanaji P. Bhopate, Prasad G. Mahajan, Govind B. Kolekar, and Shivajirao R. Patil. "TNPs as a novel fluorescent sensor for the selective recognition of fast green FCF: a spectrofluorimetric approach." RSC Advances 5, no. 85 (2015): 69371–77. http://dx.doi.org/10.1039/c5ra09835a.

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The fast green FCF dye adsorbed over the surface of the CTAB stabilized tetracene nanoparticles (TNPs) forms a stable, non-fluorescent ground state complex and quenches fluorescence of nanoparticle sensor.
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Song, Xiaofang, Lifo Ruan, Tianyu Zheng, Jun Wei, Jiayu Zhang, Huiru Lu, Huiru Lu, Yi Hu, Jun Chen, and Yanan Xue. "A Reduction Active Theranostic Nanoparticle for Enhanced Near-Infrared Imaging and Phototherapy by Reducing Glutathione Level in Cancer Cells." Journal of Nanoscience and Nanotechnology 21, no. 12 (December 1, 2021): 5965–71. http://dx.doi.org/10.1166/jnn.2021.19514.

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Facile preparation of a tumoral-stimuli-activated theranostic nanoparticle with simple constituents remains a challenge for tumor theranostic nanosystems. Herein we design a simple reductionresponsive turn-on theranostic nanoparticle for achieving fluorescent imaging and phototherapy combination. The theranostic nanoparticle is prepared by a simple one-step dialysis method of reduction active amphiphilic hyperbranched poly(β-amidoamines) and a near-infrared (NIR) dye indocyanine green (ICG). The fluorescence of ICG is quenched by the aggregation-caused quenching (ACQ) effect. The fluorescent intensity of free ICG at 816 nm was ∼40 times as high as that of particulate ICG. After reductive nanoparticles incubated with dithiothreitol (DTT), the size of the nanoparticles increased from 160 nm to 610 nm by Dynamic light scattering (DLS). As nanoparticles were internalized by cancer cells, the disulfide bonds would be cleaved by intracellular reduction agents like glutathione (GSH), leading to the release of entrapped ICG. The released ICG regained its fluorescence for self-monitoring the release and therapeutic effect of ICG by fluorescence spectra and the quantitative evaluation of NIR fluorescence intensity. Remarkably, nanoparticles can also reinforce antitumor efficacy through photodynamic therapy and GSH depletion property. This study provides new insights into designing turn-on theranostic systems.
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Thompson, Shelby, Mychele Jorns, and Dimitri Pappas. "Synthesis and Characterization of Dye-Doped Au@SiO2 Core-Shell Nanoparticles for Super-Resolution Fluorescence Microscopy." Applied Spectroscopy 76, no. 11 (October 24, 2022): 1367–74. http://dx.doi.org/10.1177/00037028221121357.

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Dye-doped nanoparticles have been investigated as bright, fluorescent probes for localization-based super-resolution microscopy. Nanoparticle size is important in super-resolution microscopy to get an accurate size of the object of interest from image analysis. Due to their self-blinking behavior and metal-enhanced fluorescence (MEF), Ag@SiO2 and Au@Ag@SiO2 nanoparticles have shown promise as probes for localization-based super-resolution microscopy. Here, several noble metal-based dye-doped core-shell nanoparticles have been investigated as self-blinking nanomaterial probes. It was observed that both the gold- and silver-plated nanoparticle cores exhibit weak luminescence under certain conditions due to the surface plasmon resonance bands produced by each metal, and the gold cores exhibit blinking behavior which enhances the blinking and fluorescence of the dye-doped nanoparticle. However, the silver-plated nanoparticle cores, while weakly luminescent, did not exhibit any blinking; the dye-doped nanoparticle exhibited the same behavior as the core fluorescent, but did not blink. Because of the blinking behavior, stochastic optical reconstruction microscopy (STORM) super-resolution analysis was able to be performed with performed on the gold core nanoparticles. A preliminary study on the use of these nanoparticles for localization-based super-resolution showed that these nanoparticles are suitable for use in STORM super resolution. Resolution enhancement was two times better than the diffraction limited images, with core sizes reduced to 15 nm using the hybrid Au–Ag cores.
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Atanasova, Milka, Yavor Ivanov, Elena Zvereva, Anatoly Zherdev, and Tzonka Godjevargova. "Simultaneous Determination of Penicillin G and Chloramphenicol in Milk by a Magnetic Nanoparticle-Based Fluorescent Immunoassay." Open Biotechnology Journal 14, no. 1 (June 16, 2020): 59–69. http://dx.doi.org/10.2174/1874070702014010059.

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Background: Antibiotic residues are a problem of increasing importance and have direct consequences for human and animal health. The frequent use of antibiotics in veterinary practice causes their excretion in milk in dairy cattle. This way, they can easily enter the human body through the consumption of milk and dairy products. Objectives: This induces the need for accurate and sensitive methods to monitor antibiotic levels in milk. The aim of this study was to develop a rapid and sensitive magnetic nanoparticle-based fluorescence immunoassay for the simultaneous detection of chloramphenicol and penicillin G in milk. Methods: Magnetic nanoparticles were synthesized and functionalized with (3-aminopropyl)triethoxysilane. Chloramphenicol-Ovalbumin and Chloramphenicol-Ovalbumin-Fluorescein-5-isothiocyanate conjugates were prepared. Penicillin G – ATTO 633 fluorescent conjugate was synthesized. Antibodies against chloramphenicol and penicillin G were immobilized onto the magnetic nanoparticles. The competitive fluorescent immunoassay was developed. The optimal concentration of the antibody-magnetic nanoparticles and the fluorescent conjugates for the assay was determined. The calibration curves for the antibiotics in buffer and milk were plotted. Fluorescent immunoassay for the simultaneous determination of chloramphenicol and penicillin G in milk was developed. Results: The limit of detection by the simultaneous immunoassay of chloramphenicol and penicillin G in milk was 0.85 ng/mL and 1.6 ng/mL, respectively. The recovery of different concentrations of chloramphenicol and penicillin G in milk samples varied from 98% to 106%. Conclusions: A rapid and sensitive magnetic nanoparticle-based immunofluorescent assay for the simultaneous determination of chloramphenicol and penicillin G in milk was developed. The magnetic nanoparticles ensured rapid and easy procedure.
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Singh Rana, Prem Jyoti, Pallavi Singh, and Prasenjit Kar. "Carbon nanoparticles for ferric ion detection and novel HFCNs–Fe3+composite for NH3and F−estimation based on a “TURN ON” mechanism." Journal of Materials Chemistry B 4, no. 35 (2016): 5929–37. http://dx.doi.org/10.1039/c6tb00975a.

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Hollow fluorescent carbon nanoparticle and solid fluorescent carbon nanoparticle were synthesised separately fromSyzygium cuminiextractviaa self-catalysis method in large scale for commercialization without providing any external heat. The nanoparticles were charaterized and their applications studied.
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Hayashi, Terutake, Masaki Michihata, Yasuhiro Takaya, and Kok Foong Lee. "Development of Nano Particle Sizing System Using Fluorescence Polarization." ACTA IMEKO 2, no. 2 (January 15, 2014): 67. http://dx.doi.org/10.21014/acta_imeko.v2i2.108.

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<p>In order to measure the sizes of nanoparticles with a wide size distribution in a solvent, we developed an optical microscopy system that allows for fluorescence polarization (FP) measurement and optical observation. This system allows the evaluation of nanoparticle sizes over a wide range, because the fluorescent signal intensity is independent of changes in the nanoparticle sizes. In this paper, we describe a fundamental experiment to verify the feasibility of using this system for different sizes of nanoparticles.</p>
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Hayashi, Terutake, Yuki Ishizaki, Masaki Michihata, Yasuhiro Takaya, and Shin-ichi Tanaka. "Study on Nanoparticle Sizing Using Fluorescent Polarization Method with DNA Fluorescent Probe." International Journal of Automation Technology 9, no. 5 (September 5, 2015): 534–40. http://dx.doi.org/10.20965/ijat.2015.p0534.

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Fluorescent polarization methods are used to detect complementary base pairing of DNA in biological fields. These methods work by measuring the rotational diffusion coefficient of Brownian motion of the fluorescent particles in solution. The rotational diffusion coefficient corresponds to the inverse third power of diameter according to the Debye-Stokes-Einstein equation for nanoparticles as hard spheres. We develop a novel method to measure the rotational diffusion coefficient using a fluorescent probe with a DNA spacer connected to a gold nanoparticle. We studied the physical characteristics of this probe to verify the feasibility of the proposed method. The rotational diffusion coefficients of gold nanoparticles with diameters ranging between 5–20 nm were measured using this developed system. In this manuscript we describe a novel fluorescent polarization method for nanoparticle sizing using a fluorescent DNA probe.
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Jenie, Aisyiyah S. N., Fransiska S. H. Krismastuti, Yudia P. Ningrum, Anis Kristiani, Mutia D. Yuniati, Widi Astuti, and Himawan T. B. M. Petrus. "Geothermal silica-based fluorescent nanoparticles for the visualization of latent fingerprints." Materials Express 10, no. 2 (February 1, 2020): 258–66. http://dx.doi.org/10.1166/mex.2020.1551.

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The development of silica nanoparticles from the waste of geothermal power plants and their subsequent modification using a fluorescent dye, rhodamine 6G (R-6G), has been reported. The optimum specific surface area of the silica nanoparticles before modification was 289.2 m2 g–1. After modification, the intrinsic properties of the fluorescent silica nanoparticles were studied, and the results showed that they were in their amorphous phase, with a particle size of 5–10 nm. We proposed that the interaction between R-6G and the silica nanoparticle surface was due to the hydrogen bonding, using the results from the Fourier transform infrared spectroscopy. The obtained fluorescent silica nanoparticles had excellent fluorescence enhancement of 2-fold compared to R-6G in its original state. This study reports, for the first time, the synthesis of fluorescent nanoparticles from geothermal silica and its ability to visualize latent fingerprints on different smooth dry surfaces, making it an excellent candidate for fluorescent powders in forensic applications.
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Dissertations / Theses on the topic "Fluorescent Nanoparticle"

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Kong, Yifei. "Multifunctional fluorescent nanoparticle-bioconjugates : preparation, characterisation and bioimaging applications." Thesis, University of Leeds, 2015. http://etheses.whiterose.ac.uk/12252/.

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Liao, Yuanyuan. "Crystals and nanoparticles of a BODIPY derivative : spectroscopy and microfluidic precipitation." Phd thesis, École normale supérieure de Cachan - ENS Cachan, 2013. http://tel.archives-ouvertes.fr/tel-00957872.

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During this work, we have addressed two aspects of the properties of the fluorescent organic nanoparticles made of Adambodipy: their spectroscopy and their production with controlled sizes. We have produced micro-crystals (100x10x1µm3) by precipitation in solutions of low supersaturation. We have measured their spectroscopy under microscope in the range 380nm to 900nm. The microcrystals are birefringent and dichroic. By adding polarizers on a microscope we have measured their refraction index along the two neutral axes according to the method of Swanepoel. We have measured the two absorption spectra along the neutral axis. We have calculated these absorption spectra using the model of the dipolar coupling for Frenkel excitons. The amplitude of this coupling has been estimated according to the classic model. But for two particular pairs of the cell, we have compared this estimation with the value that can be deduced from the quantum calculation of a dimer by TDDFT. The calculated spectra reproduce the dichroism, the spectral broadening of the absorption spectra but not the experimental peak shape probably because our micro-spectrophotometer levels up at high absorbance. The calculated fluorescence spectra predict a polarized transition along the b direction of the cell. The experiment shows two other red shifted bands. The study of their polarization, as well as their fluorescence lifetime allows us to attribute them to defects in the crystal. The spectra of the nanoparticles produced in the second part of this work are not those of crystals. We have been able to reproduce them theoretically by introducing an orientation disorder inside the periodic structure. The 3D hydrodynamic focusing enables us to produce nanoparticles with controlled size without precipitation of Adambodipy on the wall. We have used the PDMS technology and we moved to a glass tube approach, in order to avoid the diffusion of fluorescence into the PDMS. By adjusting the flow ratio between the inner organic solution of the dye and outer aqueous solution, we can control the size of the nanoparticle between 100nm and 300nm. The stability of the colloidal suspension is maintained by the surfactant CTACl below the CMC. Indeed above the CMC, the nanoparticles exist together with dyes dispersed in micelles. We have simulated using COMSOL the precipitation of the nanoparticles. We have introduced in the calculation the hydrodynamic and mutual diffusion of water and ethanol, as well as the diffusion of the Adambodipy. From our studies of the solubility of Adambodipy in water/ethanol mixtures, we have obtained the saturation curve and we have built the supersaturation maps in the micro-device. We have used Fluorescence lifetime imaging microscopy to follow in situ the precipitation process. From the decay collected in different positions can be attributed to the coexistence of three species : the monomers, the nanoparticles and an intermediate species supposed to be the nuclei. The FLIM shows a precipitation in the diffusion area of the two solvents as well as a massive precipitation after a few hundred of millisecond. The FLIM images are very close to the COMSOL predictions.
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Thakur, Dhananjay P. "Fluorescent and Magnetic Nanocomposites for Multimodal Imaging." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1274630209.

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Poncheri, Adam James. "Plasmonic field effects of silver nanoparticle monolayers on poly(phenylene ethynylene) fluorescent polymers of different chain length." Thesis, Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/41099.

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The literature on nanomaterials has been flooded with new shapes, sizes, and compositions of nanostructures. The process of developing and characterizing these particles has been broadly accomplished and many interesting and promising properties have been revealed for application in current and developing technologies. In particular, the phenomenon of surface plasmon resonance seen in metallic gold and silver nanoparticles has drawn substantial interest. It has been established that the electromagnetic fields surrounding plasmonic particle surfaces can influence the properties of nearby systems, causing them to experience effects such as enhanced absorption and emission of light or drastically increased conductivity. For this reason, plasmonic nanoparticles are being applied to an endless number of applications for new materials. This thesis investigated the effects of silver nanocube (AgNC) arrays on the photophysical properties of poly(paraphenyleneethynylene) (PPE) fluorescent polymers, a particularly relevant material to the applications of organic-electronics. AgNCs were selected because of their particularly strong plasmonic field, which is enhanced at the sharp features of the cubes. The PPE polymer is an exceptionally fluorescent conjugated polymer that often serves as a building block for polymer-based sensing applications. By monitoring the absorption and emission of the PPE polymer, a better understanding of plasmonic effects on this polymer system was obtained. Compression of the monolayer of AgNCs on the surface of a Langmuir-Blodgett trough can be used for control of interparticle distance and, thus, the plasmon field intensity felt by an adsorbed layer of PPE polymer. In the Chapter 4, PPE (n = 15) emission was monitored as a function of the AgNC plasmonic field. A two-photon process was found to explain the unusual increase then decrease of the fluorescence intensity. This observation was attributed to exciton-exciton annihilation processes within the polymer. The annihilation process is initiated by large enhancements of the polymer absorption rate when plasmonic fields are at their highest (when the AgNCs are compressed to short interparticle distances). In chapter 5, the optical properties of PPE polymers as a function of their chain length and the AgNC density were examined. A simple study was conducted to consider the conformational/geometrical effects on PPE that were caused by the deposition of PPE onto the AgNC topography. In this study, the structure of the absorption and emission profiles were evaluated and used as evidence of polymer interchain interactions, planarization, and even the potential generation of oligomeric species through breaking of conjugation. Fundamental interactions between materials must be evaluated and optimized prior to their use in devices. This thesis serves to shed a little bit of light on the interaction of a well-defined plasmonic particle with a conjugated polymer. The Langmuir-Blodgett technique serves as a critical tool in applying these colloidally produced nanoparticles to 2D arrays in practical applications. The observation of exciton-exciton annihilation at low-energy excitation is an entirely new phenomenon that was initiated by the plasmonic properties of metal nanoparticles. It is the hope of the author that the results contained herein can aide in the use of plasmonic nanoparticles in future devices.
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Khandelwal, P. "Understanding the nucleation and growth mechanism of metal nanoparticles and fluorescent metal quantum clusters and their applications." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 2017. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/4518.

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Patel, Sandeep A. "Photophysics of fluorescent silver nanoclusters." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/28110.

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Thesis (M. S.)--Chemistry and Biochemistry, Georgia Institute of Technology, 2009.
Committee Chair: Dickson, Robert; Committee Member: Brown, Ken; Committee Member: Curtis, Jennifer; Committee Member: Payne, Christine; Committee Member: Perry, Joseph.
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Agrawal, Amit. "Nanoparticle Probes for Ultrasensitive Biological Detection and Motor Protein Tracking inside Living Cells." Diss., Georgia Institute of Technology, 2006. http://hdl.handle.net/1853/19798.

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Semiconductor quantum dots (QDs) have emerged as a new class of fluorescent probes and labeling agents for biological samples. QDs are bright, highly photostable and allow simultaneous excitation of multiple emissions. Owing to these properties, QDs hold exceptional promise in enabling intracellular biochemical studies and diagnosis with unprecedented sensitivity and accuracy. However, use of QD probes inside living cells remains a challenge due to difficulties in delivery of nanoparticles without causing aggregation and imaging single nanoparticles inside living cells. In this dissertation, a systematic approach to deliver, image and locate single QDs inside living cells is presented and the properties of molecular motor protein driven QD transport are studied. First, spectroscopic and imaging methods capable of differentiating single nanoparticles from the aggregates were developed. These technologies were validated by differentiating surface protein expression on viral particles and by enabling rapid counting of single biomolecules. Second, controlled delivery of single QDs into living cells is demonstrated. A surprising finding is that single QDs associate non-specifically with the dynein motor protein complex and are transported to the microtubule organizing center. Accurate localization and tracking of QDs inside cell cytoplasm revealed multiple dynein motor protein attachment resulting in increased velocity of the QDs. Further, spectrin molecule which is known to recruit dynein motor protein complex to phospholipid micelles was found to associate with the QDs. These results may serve as a benchmark for developing new QD surface coatings suitable for intracellular applications. Since, nanoparticles are similar in size to viral pathogens; better understanding of nanoparticle-cell interactions should also help engineer nanoparticle models to study virus-host cell interactions. (Contains AVI format multimedia files)
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McCracken, Christie Joy. "Toxicity of Food-Relevant Nanoparticles in Intestinal Epithelial Models." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437688702.

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Cho, Hoon-Sung. "Design and Development of a multifunctional nano carrier system for imaging, drug delivery, and cell targeting in cancer research." University of Cincinnati / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1275936260.

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Hajjaji, Hamza. "Nanosondes fluorescentes pour l'exploration des pressions et des températures dans les films lubrifiants." Thesis, Lyon, INSA, 2014. http://www.theses.fr/2014ISAL0076/document.

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L’objectif de ce travail est d’utiliser les nanoparticules (NPs) de nanosondes fluorescentes de température en particulier dans les films lubrifiants. Le développement de ces nanosondes nécessite la détermination de leurs sensibilités thermiques afin de pouvoir sélectionner les NPs les plus prometteuses. Pour atteindre cet objectif, nous avons présenté deux méthodes d’élaboration utilisées pour la synthèse des nanostructures à base de SiC-3C, la méthode d’anodisation électrochimique et la méthode d’attaque chimique. Dans le premier cas, les analyses FTIR,RAMAN et MET des NPs finales ont montré que la nature chimique de ces NPs est majoritairement formée de carbone graphitique. L’étude détaillée de la photoluminescence de ces NPs a montré que le processus d’émission dépend de la chimie de surface des NPs, du milieu de dispersion et de sa viscosité, de la concentration des suspensions et de la température du milieu. Pour la deuxième famille de NP de SiC, les analyses cohérentes MET, DLS et PL ont montrées une taille moyenne de 1.8 nm de diamètre avec une dispersion de ±0.5nm. Le rendement quantique externe de ces NPs est de l’ordre de 4%. Les NPs dispersées dans l’éthanol, n’ont pas montré une dépendance à la température exploitable pour notre application. Par contre, les NPs de SiC produites par cette voie, étant donné la distribution en taille resserrée et le rendement quantique « honorable » pour un matériau à gap indirect, sont prometteuses pour des applications comme luminophores en particulier pour la biologie grâce à la non toxicité du SiC. Dans le cas des NPs de Si, nous avons également étudié deux types différents de NPs. Il s’agit de : (i) NPs obtenues par anodisation électrochimique et fonctionnalisées par des groupements alkyls (décène, 1-octadécène). Nous avons mis en évidence pour la première fois une très importante variation de l’énergie d’émission dEg/dT avec la température de type red-shift entre 300 et 400K. Les mesures de(T) conduisent à une sensibilité thermique de 0.75%/°C tout à fait intéressante par rapport aux NPs II-VI. De plus il a été montré que la durée de vie mesurée n’est pas fonction de la concentration. (ii) NPs obtenue par voie humide et fonctionnalisées par le n-butyl. Pour ce type de NPs nous avons mis pour la première fois en évidence un comportement de type blue-shift pour dEg/dT de l’ordre de -0.75 meV/K dans le squalane. Pour ces NPs, la sensibilité thermique pour la durée de vie de 0.2%°C est inférieure à celle des NPs de type (i) mais largement supérieure à celle des NPs de CdSe de 4 nm (0.08%/°C). La quantification de cette la sensibilité à la température par la position du pic d’émission dEg/dT et de la durée de vie nous permet d’envisager la conception de nanosondes de température basée sur les NPs de Si avec comme recommandations l’utilisation de NPs obtenues par anodisation électrochimique et de la durée de vie comme indicateur des variations en température
The goal of this study is the use of Si and SiC nanoparticles (NPs) as fluorescent temperature nanoprobes particularly in lubricating films. The development of these nanoprobes requires the determination of their thermal sensitivity in order to select the best prospects NPs. To achieve this goal, we presented two preparation methods used for the synthesis of 3C-SiC based nanostructures : (i) anodic etching method and (ii) chemical etching method. In the first case, the FTIR, Raman and TEM analysis of final NPs showed that the chemical nature of these NPs is formed predominantly of graphitic carbon. The detailed photoluminescence study of these NPs showed that the emission process depends on the surface chemistry of the NPs, the dispersion medium and its viscosity, the suspension concentration and temperature of the environment.. In the second case, coherent TEM, DLS and PL analyzes showed an average size of 1.8 nm in diameter with a dispersion of ±0.5 nm. The external quantum efficiency of these NPs is 4%. NPs dispersed in ethanol, did not show an exploitable fluorescence dependence on temperature for our application. On the other hand, 3C-SiC NPs produced by this way, given the narrow size distribution and the reasonably high quantum yield for an indirect bandgap material, are promising for applications such as luminophores in particular in the biology field thanks to nontoxicity of SiC. In the case of Si we studied also two different types of NPs. (i) NPs obtained by anodic etching and functionalized by alkyl groups (decene, octadecene). We have demonstrated for the first time an important red-shift in the emission energy dEg/dT with temperature from 300 to 400K. The PL lifetime measurement(T) lead to a thermal sensitivity of 0.75% /°C very interesting compared to II-VI NPs. Furthermore it has been shown that t is not depending on the concentration. (ii) NPs obtained by wet-chemical process and functionalized with n-butyl. For this type of NPs we have identified for the first time a blue-shift behavior of dEg dT in the order of -0.75 meV/K in squalane. The thermal sensitivity for the PL lifetime of these NPs is 0.2%/°C, which is lower than that of NPs obtained by anodic etching method, but much greater than that of CdSe NPs with 4 nm of diameter (0.08%/°C). Quantification of the temperature sensitivity by the position of emission peak dEg/dT and the PL lifetime dτ/dT allows us to consider the realization of temperature nanoprobes based on Si NPs with recommendations to use Si NPs obtained by anodic etching method and PL lifetime as an indicator of temperature changes
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Books on the topic "Fluorescent Nanoparticle"

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Wani, Waseem A., Mohammad Shahid, Afzal Hussain, and Mohamed Fahad AlAjmi. Fluorescent Organic Nanoparticles. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2655-4.

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Geddes, Chris D. Metal-enhanced fluorescence. Hoboken, N.J: Wiley, 2010.

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Geddes, Chris D. Metal-enhanced fluorescence. Hoboken, N.J: Wiley, 2010.

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D, Geddes Chris, ed. Metal-enhanced fluorescence. Hoboken, N.J: Wiley, 2010.

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Demchenko, Alexander P. Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles. Berlin, Heidelberg: Springer-Verlag Berlin Heidelberg, 2010.

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Raghavachari, Ramesh, and Samuel Achilefu. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications V: 4-6 February 2013, San Francisco, Calififornia, United States. Edited by SPIE (Society), SPIE Photonics West (Conference) (2013 : San Francisco, Calif.), and Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications (Conference) (5th : 2013 : San Francisco, Calif.). Bellingham, Washington: SPIE, 2013.

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Raghavachari, Ramesh, and Samuel Achilefu. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications IV: 23-25 January 2012, San Francisco, California, United States. Edited by SPIE (Society). Bellingham, Wash: SPIE, 2012.

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Achilefu, Samuel. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications: 26-29 January 2009, San Jose, California, United States. Bellingham, Wash: SPIE, 2009.

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(Society), SPIE, ed. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications: 26-29 January 2009, San Jose, California, United States. Bellingham, Wash: SPIE, 2009.

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Raghavachari, Ramesh, and Samuel I. Achilefu. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applicaitons II: 25-27 January 2010, San Francisco, California, United States. Bellingham, Wash: SPIE, 2010.

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Book chapters on the topic "Fluorescent Nanoparticle"

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Zhao, Wenjun, Lin Wang, and Weihong Tan. "Fluorescent Nanoparticle for Bacteria and DNA Detection." In Bio-Applications of Nanoparticles, 129–35. New York, NY: Springer New York, 2007. http://dx.doi.org/10.1007/978-0-387-76713-0_10.

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Saha, Arindam, SK Basiruddin, and Nikhil Ranjan Jana. "Plasmonic-Fluorescent and Magnetic-Fluorescent Composite Nanoparticle as Multifunctional Cellular Probe." In Surface Plasmon Enhanced, Coupled and Controlled Fluorescence, 1–11. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2017. http://dx.doi.org/10.1002/9781119325161.ch1.

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Kang, Kyung Aih, and Mai-Dung Nguyen. "Gold Nanoparticle-Based Fluorescent Contrast Agent with Enhanced Sensitivity." In Advances in Experimental Medicine and Biology, 399–407. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-55231-6_52.

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Sun, B. Q., G. Sh Yi, W. L. Xing, D. P. Chen, Y. X. Zhou, and J. Cheng. "Protein Array Detection with Nanoparticle Fluorescent Probes by Laser Confocal Scanning Fluorescence Detection." In Biochips, 91–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-662-05092-7_8.

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Wang, Jianting, Martin O’Toole, Archna Massey, Souvik Biswas, Michael Nantz, Samuel Achilefu, and Kyung A. Kang. "Highly Specific, NIR Fluorescent Contrast Agent with Emission Controlled by Gold Nanoparticle." In Oxygen Transport to Tissue XXXII, 149–54. Boston, MA: Springer US, 2011. http://dx.doi.org/10.1007/978-1-4419-7756-4_21.

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Jiang, Shan, Kornelia Gawlitza, and Knut Rurack. "Dual-Fluorescent Nanoparticle Probes Consisting of a Carbon Nanodot Core and a Molecularly Imprinted Polymer Shell." In Molecularly Imprinted Polymers, 195–208. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1629-1_17.

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Chawla, Santa. "Nanoparticles and Fluorescence." In Handbook of Nanoparticles, 961–83. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-15338-4_43.

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Chawla, Santa. "Nanoparticles and Fluorescence." In Handbook of Nanoparticles, 1–19. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-13188-7_43-1.

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Mondal, Somen, and Pradipta Purkayastha. "Hollow Fluorescent Carbon Nanoparticles." In Carbon Nanomaterials Sourcebook, 353–62. Boca Raton : Taylor & Francis Group, 2016. | “A CRC title.” |: CRC Press, 2018. http://dx.doi.org/10.1201/9781315371337-16.

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Chen, Xiaokai, Xiaodong Zhang, and Fu-Gen Wu. "Silicon Nanoparticles for Cell Imaging." In Fluorescent Materials for Cell Imaging, 77–95. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-5062-1_4.

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Conference papers on the topic "Fluorescent Nanoparticle"

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Heller, Michael J., Dieter Dehlinger, Sadik Esener, and Benjamin Sullivan. "Electric Field Directed Fabrication of Biosensor Devices From Biomolecule Derivatized Nanoparticles." In ASME 2007 2nd Frontiers in Biomedical Devices Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/biomed2007-38093.

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An electronic microarray has been used to carry out directed self-assembly of higher order 3D structures from Biotin/Streptavidin and DNA derivatized nanoparticles. Structures with more than forty layers of alternating biotin and streptavidin and DNA nanoparticles were fabricated using a 400 site CMOS microarray system. In this process, reconfigurable electric fields produced by the microarray device have been used to rapidly transport, concentrate and accelerate the binding of 40 and 200 nanometer biotin, streptavidin, DNA and peroxidase derivatized nanoparticles to selected sites on the microarray. The nanoparticle layering process takes less than one minute per layer (10–20 seconds for addressing and binding nanoparticles, 40 seconds for washing). The nanoparticle addressing/binding process can be monitored by changes in fluorescence intensity as each nanoparticle layer is deposited. The final multilayered 3-D structures are about two microns in thickness and 50 microns in diameter. Work is now focused on assembling “micron size” biosensor devices from bio-molecule derivatized luminescent and fluorescent nanoparticles. The proposed structure for a nanolayered glucose sensor device includes a base layer of biotin/streptavidin nanoparticles, a layer of glucose oxidase derivatized nanoparticles, a layer of peroxidase derivatized nanoparticles, a layer of quantum dots, and a final layer of biotin/streptavidin nanoparticles. Such a device will serve as a prototype for a wide variety of applications which includes other biosensor devices, lab-on a-chip devices, in-vivo drug delivery systems and “micron size” dispersible bio/chem sensors for environmental, military and homeland security applications.
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Nandy, Papiya, Debbethi Bera, Kunal Pal, Parimal Karmakar, and Sukhen Das. "Highly Fluorescent Carbon Nanoparticle: An Emerging Bioimaging Intervention." In MOL2NET 2020, International Conference on Multidisciplinary Sciences, 6th edition. Basel, Switzerland: MDPI, 2020. http://dx.doi.org/10.3390/mol2net-06-06786.

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Zhong, Xin, and Fei Duan. "Nanoparticle Motion and Deposition Pattern From Evaporating Binary Droplets." In ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/mnhmt2016-6477.

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The effect of ethanol in the binary solution sessile droplet is investigated on the flow field, nanoparticle motion and nanoparticle deposition pattern. It is found that the droplets with ethanol exhibited three distinct flow regimes through the Particle Image Velocimetry (PIV) analysis on the flow field of droplets suspended with fluorescent microspheres. Regime I features furious flows and vortices which transport particles to the liquid-vapor interface and make them aggregate. In regime II, the aggregates of particles move towards the central area of the droplet dominated by Marangoni flow led by non-uniformity of ethanol along the droplet surface. As the droplet enters regime III, most ethanol has evaporated and it is dominated by the drying of the remaining water. The loading of ethanol in the solution prolongs the relative durations of regimes I and II, resulting in the variety of the final drying pattern of nanoparticles.
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Xue, Jianpeng, Zeqing Li, Hanmei Xu, and Yang Pu. "A novel fluorescent gold nanoparticle inhibiting migration and invasion of tumor cells." In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI, edited by Samuel Achilefu and Ramesh Raghavachari. SPIE, 2019. http://dx.doi.org/10.1117/12.2508636.

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Zyubin, Andrey, Vladimir Rafalskiy, Karina I. Matveeva, Ekaterina Moiseeva, Alina Tsapkova, Elizaveta Demishkevich, Ilia G. Samusev, and Valery Bryukhanov. "Photophysical properties of nanoparticle-dye-protein complexes for fluorescent labeling purposes." In Plasmonics V, edited by Zheyu Fang and Takuo Tanaka. SPIE, 2020. http://dx.doi.org/10.1117/12.2575386.

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Culhane, Kyle M., Kathrin Spendier, and Anatoliy O. Pinchuk. "Functionalized fluorescent silver nanoparticle surfaces for novel sensing and imaging techniques." In SPIE Sensing Technology + Applications, edited by Nibir K. Dhar and Achyut K. Dutta. SPIE, 2015. http://dx.doi.org/10.1117/12.2177195.

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Jiang, Liwen, Xuqing Sun, Hongyao Liu, Wei Xiong, Yaqin Chen, and Xinchao Lu. "Label-free imaging to single nanoparticle by using TIR-based Interface Scattering." In JSAP-OSA Joint Symposia. Washington, D.C.: Optica Publishing Group, 2017. http://dx.doi.org/10.1364/jsap.2017.8a_a409_2.

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Total Internal Reflection (TIR) microscopy combining fluorescent probe has been widely used in the bio-imaging. As the bleaching and quenching introduced by the fluorescent probe, people kept on looking for the label-free microscopic method. Studies on label-free imaging has been implemented by collecting the space scattering of evanescent wave excited by total internal reflection, and imaging to ~40 nm single gold nanoparticle and ~ 100 nm single virus has been achieved [1-2]. Here, we introduced a novel label-free TIR-based Interface Scattering approach, which images the single nanoparticle by using the interface scattering of evanescent wave. The evanescent wave excited by TIR-based illumination, then the nanoparticle polarizes and emits the scattering which includes both space and interface scattering (shown in Figure 1). The interface scattering interferes with the incident evanescent wave is collected for imaging. We presented the imaging of single 200nm and 100nm polystyrene nanoparticle (shown in Figure 2). This approach is potential for application in fast, in-situ, label-free nanoscale imaging.
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Chen, Kok Hao, and Jong Hyun Choi. "Nanoparticle-Aptamer: An Effective Growth Inhibitor for Human Cancer Cells." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-11966.

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Semiconductor nanocrystals have unique optical properties due to quantum confinement effects, and a variety of promising approaches have been devised to interface the nanomaterials with biomolecules for bioimaging and therapeutic applications. Such bio-interface can be facilitated via a DNA template for nanoparticles as oligonucleotides can mediate the aqueous-phase nucleation and capping of semiconductor nanocrystals.[1,2] Here, we report a novel scheme of synthesizing fluorescent nanocrystal quantum dots (NQDs) using DNA aptamers and the use of this biotic/abiotic nanoparticle system for growth inhibition of MCF-7 human breast cancer cells for the first time. Particularly, we used two DNA sequences for this purpose, which have been developed as anti-cancer agents: 5-GGT GGT GGT GGT TGT GGT GGT GGT GG-3 (also called, AGRO) and 5-(GT)15-3.[3–5] This study may ultimately form the basis of unique nanoparticle-based therapeutics with the additional ability to optically report molecular recognition. Figure 1a shows the photoluminescence (PL) spectra of GT- and AGRO-passivated PbS QD that fluoresce in the near IR, centered at approximately 980 nm. A typical synthesis procedure involves rapid addition of sodium sulfide in the mixture solution of DNA and Pb acetate at a molar ratio of 2:4:1. The resulting nanocrystals are washed to remove unreacted DNA and ions by adding mixture solution of NaCl and isopropanol, followed by centrifugation. The precipitated nanocrystals are collected and re-suspended in aqueous solution by mild sonication. Optical absorption measurements reveal that approximately 90 and 77% of GT and AGRO DNA is removed after the washing process. The particle size distribution in Figure 1b suggests that the GT sequence-capped PbS particles are primarily in 3–5 nm diameter range. These nanocrystals can be easily incorporated with mammalian cells and remain highly fluorescent in sub-cellular environments. Figure 1c serially presents an optical image of a MCF-7 cell and a PL image of the AGRO-capped QD incorporated with the cell. Figure 1. (a) Normalized fluorescence spectra of PbS QD synthesized with GT and AGRO sequences, which were previously developed as anti-cancer agents. The DNA-capped QD fluoresce in the near IR centered at ∼980 nm. (b) TEM image of GT-templated nanocrystals ranging 3–5 nm in diameter. (c) Optical image of an MCF-7 human breast cancer cell after a 12-hour exposure to aptamer-capped QD. (d) PL image of AGRO-QD incorporated with the cell, indicating that these nanocrystals remain highly fluorescent in sub-cellular environments. One immediate concern for interfacing inorganic nanocrystals with cells and tissue for labeling or therapeutics is their cytotoxicity. The nanoparticle cytotoxicity is primarily determined by material composition and surface chemistry, and QD are potentially toxic by generating reactive oxygen species or by leaching heavy metal ions when decomposed.[6] We examined the toxicity of aptamer-passivated nanocrystals with NIH-3T3 mouse fibroblast cells. The cells were exposed to PbS nanocrystals for 2 days before a standard MTT assay as shown in Figure 2, where there is no apparent cytotoxicity at these doses. In contrast, Pb acetate exerts statistically significant toxicity. This observation suggests a stable surface passivation by the DNA aptamers and the absence of appreciable Pb2+ leaching. Figure 2. Viability of 3T3 mouse fibroblast cells after a 2-day exposure to DNA aptamer-capped nanocrystals. There is no apparent dose-dependent toxicity, whereas a statistically significant reduction in cell viability is observed with Pb ions. Note that Pb acetate at 133 μM is equivalent to the Pb2+ amount that was used for PbS nanocrystal synthesis at maximum concentration. Error bars are standard deviations of independent experiments. *Statistically different from control (p&lt;0.005). Finally, we examined if these cyto-compatible nanoparticle-aptamers remained therapeutically active for cancer cell growth inhibition. The MTT assay results in Figure 3a show significantly decreased growth of breast cancer cells incorporated with AGRO, GT, and the corresponding templated nanocrystals, as anticipated. In contrast, 5-(GC)15-3 and the QDs synthesized with the same sequence, which were used as negative controls along with zero-dose control cells, did not alter cell viability significantly. Here, we define the growth inhibition efficacy as (100 − cell viability) per DNA of a sample, because the DNA concentration is significantly decreased during the particle washing. The nanoparticle-aptamers demonstrate 3–4 times greater therapeutic activities compared to the corresponding aptamer drugs (Figure 3b). We speculate that when a nanoparticle-aptamer is internalized by the cancer cells, it forms an intracellular complex with nucleolin and nuclear factor-κB (NF-κB) essential modulator, thereby inhibiting NF-κB activation that would cause transcription of proliferation and anti-apoptotic genes.[7] The nanoparticle-aptamers may more effectively block the pathways for creating anti-apoptotic genes or facilitate the cellular delivery of aptamers via nanoparticle uptake. Our additional investigation indicates that the same DNA capping chemistry can be utilized to produce aptamer-mediated Fe3O4 nanocrystals, which may be potentially useful in MRI and therapeutics, considering their magnetic properties and biocompatibility. In summary, the nanoparticle-based therapeutic schemes developed here should be valuable in developing a multifunctional drug delivery and imaging agent for biological systems. Figure 3. Anti-proliferation of MCF-7 human breast cancer cells with aptamer-passivated nanocrystals. (a) Viability of MCF-7 cells exposed to AGRO and GT sequences, and AGRO-/GT-capped QD for 7 days. The DNA concentration was 10 uM, while the particles were incubated with cells at 75 nM. (b) Growth inhibition efficacy is defined as (100 − cell viability) per DNA to correct the DNA concentration after particle washing.
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Zyubin, Andrey Y., Konstantin Alexandrov, Karina Y. Matveeva, and Ilia Samusev. "Plasmon-enhanced fluorescence of nanoparticle-dye-protein complex as perspective approach for increase in fluorescent labeling effectiveness." In Nanophotonics and Micro/Nano Optics V, edited by Zhiping Zhou, Kazumi Wada, and Limin Tong. SPIE, 2019. http://dx.doi.org/10.1117/12.2536407.

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Shang, Li, and Gerd Ulrich Nienhaus. "Fluorescent nanoparticle interactions with biological systems: What have we learned so far?" In SPIE BiOS, edited by Wolfgang J. Parak, Marek Osinski, and Xing-Jie Liang. SPIE, 2015. http://dx.doi.org/10.1117/12.2075722.

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Reports on the topic "Fluorescent Nanoparticle"

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Lu, Dengwei, Enjie Tang, Supeng Yin, Yizeng Sun, Yuquan Yuan, Tingjie Yin, Zeyu Yang, and Fan Zhang. Intraoperative strategies in identification and functional protection of parathyroid gland for patients with thyroidectomy: A network meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, November 2022. http://dx.doi.org/10.37766/inplasy2022.11.0109.

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Review question / Objective: To assess the roles of intraoperative visualization of parathyroid glands (IVPG) strategies including autofluorescence (AF), indocyanine green (ICG) fluorescence and carbon nanoparticles (CN) in identification and functional protection of parathyroid glands (PGs). Condition being studied: The IVPG strategy is essential for intraoperative identification and functional protection of PGs in patients undergoing thyroid surgery. Recent studies have revealed that autofluorescence (AF), indocyanine green (ICG) fluorescence, and carbon nanoparticles (CN) contribute to reducing the incidence of postoperative hypocalcemia by improving PGs identification(6-8). However, different IVPGs have respective superiority and inferiority in clinical application. Thus, this network meta-analysis aimed to systematically analyze the significance of IVPG strategy in identifying PGs and protecting their function.
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Chiu, Sheng-Kuei. Photoluminescent Silicon Nanoparticles: Fluorescent Cellular Imaging Applications and Photoluminescence (PL) Behavior Study. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.2453.

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Choudhary, Ruplal, Victor Rodov, Punit Kohli, John D. Haddock, and Samir Droby. Antimicrobial and antioxidant functionalized nanoparticles for enhancing food safety and quality: proof of concept. United States Department of Agriculture, September 2012. http://dx.doi.org/10.32747/2012.7597912.bard.

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General concept. The reported 1-year study tested the feasibility ofpreparing antimicrobial and antioxidant nanoparticlesfunctionalized with natural phenolic compounds, as a first step to reach the ultimate goal - improving safely and quality of foods by developing novel antimicrobial and antioxidant food-contacting materials. The secondary objectives of the study were (a) selecting the most promising phenoliccompounds, (b) building nanoparticles with the selected phenolicgrafted on their Surface, and (c) testing antimicrobial and antioxidant properties of these particles. The study was expected to provide a " go/no go" decision as concerning the prospects of phenolic- bound nanoparticles as antimicrobial and antioxidant agents. Results. In course of the feasibility study, curucminwas chosen as the most promising phenoliccompound due to its high antibacterial activity exceeding other tested compounds by at leas one order of magnitude. Lipsome-typephospholipid/polydiacetylene(PDA) nanoparticlesfunctionalizedwith curcuminwere successfully built. The pitfall of limited curcumin amount that could be covalently bound to theparticle surface was circumvented by inclusion of curcunun in the liposome body. It was suggested onthe basis of fluorescence spectroscopy that curcuminwas bound by hydrophobic forces in the bi1ayer periphery of the Liposomesand therefore mightexert a contact effect on microorganisms. The curcumin­ functionalizednanoparticles(CFN) were shown to have a strong bactericidal activity towards both Gram-negative (E. coli) and Gram-positive (B. ce,·e11s) bacteria, but only limited effect against yeast. Furthermore, beyond the originallyplanned objectives, preliminary trials showed that CFN could be bound to silanized glass surface rendering aנבtiנnicrobial activity to the glass. Tnaddition, the particles showed antioxidantcapacity. Tberefore, it ,vas co11cluded tlוattlוeaims of tlוefeasibility study bad been successfully reached an
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