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1

Callis, P. R., and A. P. Demchenko. Advanced fluorescence reporters in chemistry and biology I: Fundamentals and molecular design. Heidelberg: Springer, 2010.

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2

service), ScienceDirect (Online, ed. Single molecule tools: Super-resolution, particle tracking, multiparameter and force based methods. San Diego, CA: Academic Press/Elsevier, 2010.

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3

Kevin, Foskett J., and Grinstein Sergio, eds. Non-invasivetechniques in cell biology. New York: Wiley-Liss, 1990.

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4

Kevin, Foskett J., and Grinstein Sergio 1950-, eds. Noninvasive techniques in cell biology. New York: Wiley-Liss, 1990.

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5

Johnson, Iain D., and Michelle T. Z. Spence. The molecular probes handbook: A guide to fluorescent probes and labeling technologies. [Carlsbad, CA]: Live Technologies Corporation, 2010.

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6

Kai, Licha, Ntziachristos Vasilis, SPIE (Society), and Optical Society of America, eds. Molecular imaging: 17-18 June 2007, Munich, Germany. Bellingham, Wash: SPIE, 2007.

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7

Liehr, Thomas. Fluorescence In Situ Hybridization (FISH) — Application Guide. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009.

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8

Pierre, Bruchez Marcel, and Hotz Charles Z, eds. Quantum dots: Applications in biology. Totowa, N.J: Humana Press, 2007.

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9

Achilefu, Samuel. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications: 26-29 January 2009, San Jose, California, United States. Bellingham, Wash: SPIE, 2009.

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10

Raghavachari, Ramesh, and Samuel I. Achilefu. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applicaitons II: 25-27 January 2010, San Francisco, California, United States. Bellingham, Wash: SPIE, 2010.

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11

Raghavachari, Ramesh, and Samuel Achilefu. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications III: 24-26 January 2011, San Francisco, California, United States. Edited by SPIE (Society). Bellingham, Wash: SPIE, 2011.

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12

(Society), SPIE, ed. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications: 26-29 January 2009, San Jose, California, United States. Bellingham, Wash: SPIE, 2009.

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13

Bensimon, David, Vincent Croquette, Jean-François Allemand, Xavier Michalet, and Terence Strick. Single-Molecule Studies of Nucleic Acids and Their Proteins. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198530923.001.0001.

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This book presents a comprehensive overview of the foundations of single-molecule studies, based on manipulation of the molecules and observation of these with fluorescent probes. It first discusses the forces present at the single-molecule scale, the methods to manipulate them, and their pros and cons. It goes on to present an introduction to single-molecule fluorescent studies based on a quantum description of absorption and emission of radiation due to Einstein. Various considerations in the study of single molecules are introduced (including signal to noise, non-radiative decay, triplet states, etc.) and some novel super-resolution methods are sketched. The elastic and dynamic properties of polymers, their relation to experiments on DNA and RNA, and the structural transitions observed in those molecules upon stretching, twisting, and unzipping are presented. The use of these single-molecule approaches for the investigation of DNA–protein interactions is highlighted via the study of DNA and RNA polymerases, helicases, and topoisomerases. Beyond the confirmation of expected mechanisms (e.g., the relaxation of DNA torsion by topoisomerases in quantized steps) and the discovery of unexpected ones (e.g., strand-switching by helicases, DNA scrunching by RNA polymerases, and chiral discrimination by bacterial topoII), these approaches have also fostered novel (third generation) sequencing technologies.
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14

Sklar, Larry A., ed. Flow Cytometry for Biotechnology. Oxford University Press, 2005. http://dx.doi.org/10.1093/oso/9780195183146.001.0001.

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Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes. Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.
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15

Lehotay, Steven John. Approaching single molecule detection by laser-induced fluorescence of flowing dye solutions in a capillary. 1992.

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16

(Editor), Charles Z. Hotz, and Marcel Bruchez (Editor), eds. Quantum Dots: Applications in Biology (Methods in Molecular Biology). Humana Press, 2007.

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17

Marcu, Laura, Paul M. W. French, and Daniel S. Elson. Fluorescence Lifetime Spectroscopy and Imaging. Taylor & Francis Group, 2020.

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18

Fluorescence Lifetime Spectroscopy and Imaging. CRC Press, 2012.

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19

Marcu, Laura, Paul M. W. French, and Daniel S. Elson. Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics. Taylor & Francis Group, 2014.

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20

Marcu, Laura, Paul M. W. French, and Daniel S. Elson. Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics. Taylor & Francis Group, 2014.

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