Relevant bibliographies by topics / Fluorescent biosensor

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Fluorescent biosensor'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "Fluorescent biosensor"

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Ma, Xing Meng, Ji Mei Zhang, Zhao Dai, Xiao Yu Chen, Xiao Qing Wang, and Qing Yin Zhang. "Fluorescent DNA Biosensors on Silica Microspheres." Advanced Materials Research 301-303 (July 2011): 195–200. http://dx.doi.org/10.4028/www.scientific.net/amr.301-303.195.

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A novel DNA biosensor system on silica microspheres as solid carriers which based on the fluorescence resonance energy transfer (FRET) was presented in this work when CdTe quantum dots (QDs) were as energy donors and Au nanoparticles (AuNPs) were as energy accepters. Compared with the fluorescent intensity of CdTe QDs, the fluorescent intensity of DNA biosensors decreased extremely, which indicated that the FRET occurred between CdTe QDs and AuNPs. The biosensor system would have a certain degree recovery of fluorescence when the complementary single stranded DNA was introduced into this system. The DNA detection results indicated that this novel fluorescent DNA probe system could recognize the existence of complementary target DNA or not.
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Liu, Xuemei, Kieran J. Germaine, David Ryan, and David N. Dowling. "DEVELOPMENT OF A GFP‐BASED BIOSENSOR FOR DETECTING THE BIOAVAILABILITY AND BIODEGRADATION OF POLYCHLORINATED BIPHENYLS (PCBS)." JOURNAL OF ENVIRONMENTAL ENGINEERING AND LANDSCAPE MANAGEMENT 15, no. 4 (December 31, 2007): 261–68. http://dx.doi.org/10.3846/16486897.2007.9636939.

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Two whole-cell biosensors were constructed to detect the in situ biodegradation of polychlorinated biphenyl by chromosomal insertion of a mini‐Tn5‐Kmr‐Pm::gfp[mut3]‐T0‐T1 construct into P. fluorescens. In vitro tests showed that the expression of the Pm promoter depended on the growth phase of the biosensors and the concentration of chemical inducers; chlorinated benzoic acid derivatives. A linear relationship between the fluorescent intensity and the log10 concentration of the inducer was observed. One biosensor (F113L::1180gfp) had the ability to degrade PCBs to relevant chlorobenzoic acid derivatives and to induce expression of Gfp. The second biosensor (F113gfp), which cannot degrade PCBs, shows fluorescence after induction by chloro‐benzoic acid derivatives. By using these two biosensors, PCB degradation could be detected in vitro and in soil.
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Tang, Longteng, Shuce Zhang, Yufeng Zhao, Nikita D. Rozanov, Liangdong Zhu, Jiahui Wu, Robert E. Campbell, and Chong Fang. "Switching between Ultrafast Pathways Enables a Green-Red Emission Ratiometric Fluorescent-Protein-Based Ca2+ Biosensor." International Journal of Molecular Sciences 22, no. 1 (January 5, 2021): 445. http://dx.doi.org/10.3390/ijms22010445.

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Ratiometric indicators with long emission wavelengths are highly preferred in modern bioimaging and life sciences. Herein, we elucidated the working mechanism of a standalone red fluorescent protein (FP)-based Ca2+ biosensor, REX-GECO1, using a series of spectroscopic and computational methods. Upon 480 nm photoexcitation, the Ca2+-free biosensor chromophore becomes trapped in an excited dark state. Binding with Ca2+ switches the route to ultrafast excited-state proton transfer through a short hydrogen bond to an adjacent Glu80 residue, which is key for the biosensor’s functionality. Inspired by the 2D-fluorescence map, REX-GECO1 for Ca2+ imaging in the ionomycin-treated human HeLa cells was achieved for the first time with a red/green emission ratio change (ΔR/R0) of ~300%, outperforming many FRET- and single FP-based indicators. These spectroscopy-driven discoveries enable targeted design for the next-generation biosensors with larger dynamic range and longer emission wavelengths.
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Lee, Woonwoo, Hyojin Kim, Yerin Kang, Youngshim Lee, and Youngdae Yoon. "A Biosensor Platform for Metal Detection Based on Enhanced Green Fluorescent Protein." Sensors 19, no. 8 (April 18, 2019): 1846. http://dx.doi.org/10.3390/s19081846.

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Microbial cell-based biosensors, which mostly rely on stress-responsive operons, have been widely developed to monitor environmental pollutants. Biosensors are usually more convenient and inexpensive than traditional instrumental analyses of environmental pollutants. However, the targets of biosensors are restricted by the limited number of genetic operon systems available. In this study, we demonstrated a novel strategy to overcome this limitation by engineering an enhanced green fluorescent protein (eGFP). It has been reported that combining two fragments of split-eGFP can form a native structure. Thus, we engineered new biosensors by inserting metal-binding loops (MBLs) between β-strands 9 and 10 of the eGFP, which then undergoes conformational changes upon interaction between the MBLs and targets, thereby emitting fluorescence. The two designed MLBs based on our previous study were employed as linkers between two fragments of eGFP. As a result, an Escherichia coli biosensor exhibited a fluorescent signal only when interacting with cadmium ions, revealing the prospect of a new biosensor for cadmium detection. Although this study is a starting stage for further developing biosensors, we believe that the proposed strategy can serve as basis to develop new biosensors to target various environmental pollutants.
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Tenner, Brian, Jason Z. Zhang, Yonghoon Kwon, Veronica Pessino, Siyu Feng, Bo Huang, Sohum Mehta, and Jin Zhang. "FluoSTEPs: Fluorescent biosensors for monitoring compartmentalized signaling within endogenous microdomains." Science Advances 7, no. 21 (May 2021): eabe4091. http://dx.doi.org/10.1126/sciadv.abe4091.

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Growing evidence suggests that many essential intracellular signaling events are compartmentalized within kinetically distinct microdomains in cells. Genetically encoded fluorescent biosensors are powerful tools to dissect compartmentalized signaling, but current approaches to probe these microdomains typically rely on biosensor fusion and overexpression of critical regulatory elements. Here, we present a novel class of biosensors named FluoSTEPs (fluorescent sensors targeted to endogenous proteins) that combine self-complementing split green fluorescent protein, CRISPR-mediated knock-in, and fluorescence resonance energy transfer biosensor technology to probe compartmentalized signaling dynamics in situ. We designed FluoSTEPs for simultaneously highlighting endogenous microdomains and reporting domain-specific, real-time signaling events including kinase activities, guanosine triphosphatase activation, and second messenger dynamics in live cells. A FluoSTEP for 3′,5′-cyclic adenosine monophosphate (cAMP) revealed distinct cAMP dynamics within clathrin microdomains in response to stimulation of G protein–coupled receptors, showcasing the utility of FluoSTEPs in probing spatiotemporal regulation within endogenous signaling architectures.
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Manonmani, V., A. Vimala Juliet, J. Ponnidevi, and P. Arumugam. "A Novel Magneto-Fluorescent Biosensor for the Detection of Pathogens in Food." Advanced Materials Research 984-985 (July 2014): 1074–79. http://dx.doi.org/10.4028/www.scientific.net/amr.984-985.1074.

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Food safety has emerged as an important global issue. Illness from the pathogenic bacteria is a significant health concern. Pathogens are potentially harmful and it contaminate food and causes foodborne illness . It is very important for early detection of food borne pathogens. Conventional methods of detecting these pathogens eventhough sensitive, but still it is time consuming,complex and laborious. Biosensors have great potential for the detection of pathogenic bacteria in food. Biosensors are developed to rapidly detect the pathogens. In this paper, we use nanosized magnetite nanoparticles coated with chitosan, a polymer to capture and identify bacteria from contaminated food sample and eliminates the purpose of specific antibody coating. Amine group with magnetite nanoparticles (MNPs) specifically binds with the bacterial cell wall, on their surface of most pathogenic bacteria present in food. Rhodamine as a dye/marker which emits fluorescence in exposure to light used to detect the bacterial concentration in terms of light or fluorescent intensity and analog voltage using Magneto-Fluorescent biosensor. The developed biosensor could be able to detect bacteria in the limit of 10 CFU/ml and the time taken for measurement of a sample using biosensor would be less than 5 minutes.Hence the developed biosensor are non-specific and highly sensitive.
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Stiner, Lawrence, and Larry J. Halverson. "Development and Characterization of a Green Fluorescent Protein-Based Bacterial Biosensor for Bioavailable Toluene and Related Compounds." Applied and Environmental Microbiology 68, no. 4 (April 2002): 1962–71. http://dx.doi.org/10.1128/aem.68.4.1962-1971.2002.

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ABSTRACT A green fluorescent protein-based Pseudomonas fluorescens strain A506 biosensor was constructed and characterized for its potential to measure benzene, toluene, ethylbenzene, and related compounds in aqueous solutions. The biosensor is based on a plasmid carrying the toluene-benzene utilization (tbu) pathway transcriptional activator TbuT from Ralstonia pickettii PKO1 and a transcriptional fusion of its promoter PtbuA1 with a promoterless gfp gene on a broad-host-range promoter probe vector. TbuT was not limiting, since it was constitutively expressed by being fused to the neomycin phosphotransferase (nptII) promoter. The biosensor cells were readily induced, and fluorescence emission after induction periods of 3 h correlated well with toluene, benzene, ethylbenzene, and trichloroethylene concentrations. Our experiments using flow cytometry show that intermediate levels of gfp expression in response to toluene reflect uniform induction of cells. As the toluene concentration increases, the level of gfp expression per cell increases until saturation kinetics of the TbuT-PtbuA1 system are observed. Each inducer had a unique minimum concentration that was necessary for induction, with K app values that ranged from 3.3 ± 1.8 μM for toluene to 35.6 ± 16.6 μM for trichloroethylene (means ± standard errors of the means), and maximal fluorescence response. The fluorescence response was specific for alkyl-substituted benzene derivatives and branched alkenes (di- and trichloroethylene, 2-methyl-2-butene). The biosensor responded in an additive fashion to the presence of multiple inducers and was unaffected by the presence of compounds that were not inducers, such as those present in gasoline. Flow cytometry revealed that, in response to toxic concentrations of gasoline, there was a small uninduced population and another larger fully induced population whose levels of fluorescence corresponded to the amount of effectors present in the sample. These results demonstrate the potential for green fluorescent protein-based bacterial biosensors to measure environmental contaminants.
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Goh, Yan Y., Bow Ho, and Jeak L. Ding. "A Novel Fluorescent Protein-Based Biosensor for Gram-Negative Bacteria." Applied and Environmental Microbiology 68, no. 12 (December 2002): 6343–52. http://dx.doi.org/10.1128/aem.68.12.6343-6352.2002.

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ABSTRACT Site-directed mutagenesis of enhanced green fluorescent protein (EGFP) based on rational computational design was performed to create a fluorescence-based biosensor for endotoxin and gram-negative bacteria. EGFP mutants (EGFPi) bearing one (G10) or two (G12) strands of endotoxin binding motifs were constructed and expressed in an Escherichia coli host. The EGFPi proteins were purified and tested for their efficacy as a novel fluorescent biosensor. After efficient removal of lipopolysaccharide from the E. coli lysates, the binding affinities of the EGFPi G10 and G12 to lipid A were established. The KD values of 7.16 × 10−7 M for G10 and 8.15 × 10−8 M for G12 were achieved. With high affinity being maintained over a wide range of pH and ionic strength, the binding of lipid A/lipopolysaccharide to the EGFPi biosensors could be measured as a concentration-dependent fluorescence quenching of the EGFP mutants. The EGFPi specifically tagged gram-negative bacteria like E. coli and Pseudomonas aeruginosa, as well as other gram-negative bacteria in contaminated water sampled from the environment. This dual function of the EGFPi in detecting both free endotoxin and live gram-negative bacteria forms the basis of the development of a novel fluorescent biosensor.
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Seitz, Kati, and Patrick J. Krysan. "Expanding the Toolkit of Fluorescent Biosensors for Studying Mitogen Activated Protein Kinases in Plants." International Journal of Molecular Sciences 21, no. 15 (July 28, 2020): 5350. http://dx.doi.org/10.3390/ijms21155350.

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Mitogen-activated protein kinases (MAPKs) are key regulators of numerous biological processes in plants. To better understand the mechanisms by which these kinases function, high resolution measurement of MAPK activation kinetics in different biological contexts would be beneficial. One method to measure MAPK activation in plants is via fluorescence-based genetically-encoded biosensors, which can provide real-time readouts of the temporal and spatial dynamics of kinase activation in living tissue. Although fluorescent biosensors have been widely used to study MAPK dynamics in animal cells, there is currently only one MAPK biosensor that has been described for use in plants. To facilitate creation of additional plant-specific MAPK fluorescent biosensors, we report the development of two new tools: an in vitro assay for efficiently characterizing MAPK docking domains and a translocation-based kinase biosensor for use in plants. The implementation of these two methods has allowed us to expand the available pool of plant MAPK biosensors, while also providing a means to generate more specific and selective MAPK biosensors in the future. Biosensors developed using these methods have the potential to enhance our understanding of the roles MAPKs play in diverse plant signaling networks affecting growth, development, and stress response.
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Hansen, Lars Hestbjerg, Belinda Ferrari, Anders Hay Sørensen, Duncan Veal, and Søren Johannes Sørensen. "Detection of Oxytetracycline Production by Streptomyces rimosus in Soil Microcosms by Combining Whole-Cell Biosensors and Flow Cytometry." Applied and Environmental Microbiology 67, no. 1 (January 1, 2001): 239–44. http://dx.doi.org/10.1128/aem.67.1.239-244.2001.

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ABSTRACT Combining the high specificity of bacterial biosensors and the resolution power of fluorescence-activated cell sorting (FACS) provided qualitative detection of oxytetracycline production byStreptomyces rimosus in soil microcosms. A plasmid containing a transcriptional fusion between thetetR-regulated P tet promoter from Tn10 and a FACS-optimized gfp gene was constructed. When harbored by Escherichia coli, this plasmid produces large amounts of green fluorescent protein (GFP) in the presence of tetracycline. This tetracycline biosensor was used to detect the production of oxytetracycline by S. rimosusintroduced into sterile soil. The tetracycline-induced GFP-producing biosensors were detected by FACS analysis, enabling the detection of oxytetracycline encounters by single biosensor cells. This approach can be used to study interactions between antibiotic producers and their target organisms in soil.
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Dissertations / Theses on the topic "Fluorescent biosensor"

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Dennis, Allison Marie. "Quantum dot-fluorescent protein pairs as fluorescence resonance energy transfer pairs." Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37079.

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Fluorescence resonance energy transfer (FRET)-based biosensors have been designed to fluorometrically detect everything from proteolytic activity to receptor-ligand interactions and structural changes in proteins. While a wide variety of fluorophores have demonstrated effectiveness in FRET probes, several potential sensor components are particularly notable. Semiconductor quantum dots (QDs) are attractive FRET donors because they are rather bright, exhibit high quantum yields, and their nanoparticulate structure enables the attachment of multiple acceptor molecules. Fluorescent proteins (FPs) are also of particular interest for fluorescent biosensors because design elements necessary for signal transduction, probe assembly, and device delivery and localization for intracellular applications can all be genetically incorporated into the FP polypeptide. The studies described in this thesis elucidate the important parameters for concerted QD-FP FRET probe design. Experimental results clarify issues of FRET pair selection, probe assembly, and donor-acceptor distance for the multivalent systems. Various analysis approaches are compared and guidelines asserted based on the results. To demonstrate the effectiveness of the QD-FP FRET probe platform, a ratiometric pH sensor is presented. The sensor, which uses the intrinsic pH-sensitivity of the FP mOrange to modulate the FP/QD emission ratio, exhibits a 20-fold change in its ratiometric measurement over a physiologically interesting pH range, making it a prime candidate for intracellular imaging applications.
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Hahn, Angela T. "Development and implementation of a live cell cycle fluorescent biosensor system /." May be available electronically:, 2007. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.

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Hung, Yin Pun. "Single Cell Imaging of Metabolism with Fluorescent Biosensors." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10147.

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Cells utilize various signal transduction networks to regulate metabolism. Nevertheless, a quantitative understanding of the relationship between growth factor signaling and metabolic state at the single cell level has been lacking. The signal transduction and metabolic states could vary widely among individual cells. However, such cell-to-cell variation might be masked by the bulk measurements obtained from conventional biochemical methods. To assess the spatiotemporal dynamics of metabolism in individual intact cells, we developed genetically encoded biosensors based on fluorescent proteins. As a key redox cofactor in metabolism, NADH has been implicated in the Warburg effect, the abnormal metabolism of glucose that is a hallmark of cancer cells. To date, however, sensitive and specific detection of NADH in the cytosol of individual live cells has been difficult. We engineered a fluorescent biosensor of NADH by combining a circularly permuted green fluorescent protein variant with a bacterial NADH-binding protein Rex. The optimized biosensor Peredox reports cytosolic \(NADH:NAD^+\) ratios in individual live cells and can be calibrated with exogenous lactate and pyruvate. Notably pH resistant, this biosensor can be used in several cultured and primary cell types and in a high-content imaging format. We then examined the single cell dynamics of glycolysis and energy-sensing signaling pathways using Peredox and other fluorescent biosensors: AMPKAR, a sensor of the AMPK activity; and FOXO3-FP, a fluorescently-tagged protein domain from Forkhead transcription factor FOXO3 to report on the PI3K/Akt pathway activity. With perturbation to growth factor signaling, we observed a transient response in the cytosolic \(NADH:NAD^+\) redox state. In contrast, with partial inhibition of glycolysis by iodoacetate, individual cells varied substantially in their responses, and cytosolic \(NADH:NAD^+\) ratios oscillated between high and low states with a regular, approximately half-hour period, persisting for hours. These glycolytic NADH oscillations appeared to be cell-autonomous and coincided with the activation of the PI3K/Akt pathway but not the AMPK pathway. These results suggest a dynamic coupling between growth factor signaling and metabolic parameters. Overall, this thesis presents novel optical tools to assess metabolic dynamics – and to unravel the elaborate and complex integration of glucose metabolism and signaling pathways at the single cell level.
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Klüßendorf, Thies [Verfasser]. "Development of a FRET-Based Fluorescent Biosensor for ERK2 Activity / Thies Klüßendorf." Dortmund : Universitätsbibliothek Technische Universität Dortmund, 2011. http://d-nb.info/1013386116/34.

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Windsor, Kramer Michelle Anne. "Development of a FRET biosensor to detect the pathogen mycoplasma capricolum." Diss., Columbia, Mo. : University of Missouri-Columbia, 2005. http://hdl.handle.net/10355/4289.

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Thesis (M.S.)--University of Missouri-Columbia, 2005.
The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file viewed on (January 11, 2006) Includes bibliographical references.
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Watabe, Tetsuya. "Booster, a Red－Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263527.

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Eason, Matthew. "A GFP-Based Sensor to Detect Transiently Expressed Proteins." Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/40500.

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Green fluorescent protein (GFP) fusion tags are commonly used to study protein expression and cellular localization in vivo. But, GFP must undergo an autogenic post-translational modification, known as chromophore maturation, to become fluorescent, a process that can have a half-time longer than 30 minutes inside research model organisms. The timescale of chromophore maturation in GFP is thus slower than many key biological processes, limiting its usefulness in measuring those processes. In this thesis, we discuss the creation and engineering of a sensor for transiently expressed proteins (STEP) based on a fully matured but dim GFP. Upon specific binding of STEPtag, a small (15.5 kDa) protein to the sensor, full fluorescence is restored. Thus, by genetically fusing STEPtag to a protein of interest, it can be detected as soon as folding is complete, without any maturation delay. Through a combination of rational design and targeted directed evolution, we describe the improvement of the original sensor, gSTEP0, into an optimized version, gSTEP1. The sensor has been validated in vitro and in E. coli cells, and we have found that for gSTEP1, the fluorescence signal increases more than three-fold upon binding, with a Kd of 120 ± 30 nM and a kon of 1.7 x 105 M-1s-1, allowing detection of the protein of interest on the second timescale. We have also created a yellow version of the biosensor, and provide preliminary attempts at developing orthogonal binding pairs, as well as red- and cyan-coloured STEPs, which could eventually be used in multiplex experiments. Our biosensor opens the door to the study of short-timescale processes in research model organisms, such as Drosophila and zebrafish embryogenesis, as well as in host-pathogen interactions, which we are currently investigating.
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Shrive, Jason David Alderson. "Artificial phospholipid membrane biosensor design, optimization of fluorescent probe loading and membrane structure." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ51531.pdf.

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Van, Ngoc. "Development of fluorescent biosensors for probing CDK/Cyclin activity in vitro and in cellulo." Thesis, Montpellier 2, 2013. http://www.theses.fr/2013MON20171.

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Les Kinases cycline-dépendantes (CDK / cyclines) jouent un rôle majeur dans la régulation de la progression du cycle cellulaire et la prolifération des cellules cancéreuses, et constituent ainsi des cibles d'intérêt pour le développement de stratégies de diagnostic et thérapeutiques anticancéreuse. L'objectif de cette étude a consisté à développer une famille de biosenseurs fluorescents pour mesurer l'activité des CDK/ cycline in vitro, in cellulo et in vivo.Nous avons conçu et développé un biosenseur polypeptidique sensible à l'environnement comprenant une séquence substrat des CDKs, qui est marquée avec une sonde fluorescente sensible à l'environnement à proximité du site de phosphorylation, et un domaine de liaison phospho-amino acide, qui se lie à la séquence du substrat lorsqu'il est phosphorylé, ce qui modifie l'environnement de la sonde fluorescente et conduit par conséquent à l'augmentation de la fluorescence. Plusieurs variants de ce premier biosenseur CDKACT ont été développés. Les biosenseurs ont d'abord été caractérisés in vitro en utilisant plusieurs complexes CDK / cycline recombinants et avec des CDK / cyclines endogènes à partir d'extraits cellulaires, induisant des changements dynamiques de l'intensité de fluorescence, qui ont été mesurés en temps réel. Nous avons caractérisé la spécificité de ces biosenseurs pour les kinases CDK/ cycline par rapport à d'autres kinases (Plk1, Plk3, CIV, PKA, MAPK). En outre ces biosenseurs permettent de mesurer des différences dans l'activité des CDK/Cyclines entre différentes lignées cellulaires saines et cancéreuses. Enfin, nous avons mis en place les conditions pour internaliser ces biosenseurs dans des cellules vivantes grâce à des formulations de peptides pénétrants, afin de mesurer l'activité des CDK / cycline en temps réel. L'imagerie time-lapse et la quantification ratiométrique de fluorescence de la sonde sensible à l'environnement par rapport à une sonde fluorescente standard a permis de suivre l'activité des CDK/ cycline au cours du cycle cellulaire de cellules en division, des cellules ne se divisant pas et des cellules traitées avec des inhibiteurs des CDK / cycline. Les biosenseurs ont également été utilisé pour établir des conditions nécessaires à réaliser un criblage haut débit et des essais d'imagerie in vivo dans des modèles de souris comportant des xénogreffes
Cyclin-dependent kinases (CDK/Cyclins) play central roles in regulation of cell cycle progression and proliferation of cancer cells, thereby constituting attractive targets for development of cancer diagnostics and therapeutics. The objective of this study consisted in developing a family of fluorescent biosensors to probe CDK/Cyclin activity in vitro, in cellulo and in vivo. To this aim, we designed and engineered an environmentally sensitive polypeptide sensor consisting of a CDK substrate sequence labelled with an environmentally-sensitive dye proximal to the phosphorylation site, and a phospho-amino acid binding domain, which binds the substrate sequence when it is phosphorylated, thereby altering the environment of the fluorescent probe and consequently leading to fluorescence enhancement. Several variants of this first CDKACT biosensor were further engineered. The biosensors were first characterized in vitro using several recombinant CDK/Cyclin complexes and endogenous CDK/Cyclins from cell extracts, inducing dynamic changes in fluorescence intensity, which were measured in real-time. We further characterized the specificity of these biosensors for CDK/Cyclin kinases as opposed to other kinases (Plk1, Plk3, CIV, PKA, MAPK). We further applied CDK biosensors to measure CDK/Cyclin kinase activity between different healthy and cancer cell lines. Finally, we established conditions to deliver the biosensors into living cells thanks to cell-penetrating peptide formulations, to monitor CDK/Cyclin activity in real time. Time-lapse imaging and ratiometric quantification of fluorescence of the environmentally sensitive probe over that of a fluorescent standard allowed to monitor CDK/Cyclin activity throughout the cell cycle of dividing cells, non-dividing cells and cells treated with CDK/Cyclin inhibitors. The biosensors were further applied to establish conditions for a high throughput screen and an in vivo imaging assay using xenografted mouse models
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Jakeway, Stephen Christopher. "Evaluation of some fluorescent dyes and molecular tethers for a fibre optic DNA biosensor." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0004/MQ45514.pdf.

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Books on the topic "Fluorescent biosensor"

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Zhang, Jin, Qiang Ni, and Robert H. Newman, eds. Fluorescent Protein-Based Biosensors. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-622-1.

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Jin, Zhang, Qiang Ni, and Robert H. Newman. Fluorescent protein-based biosensors: Methods and protocols. New York: Humana Press, 2014.

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S, Wolfbeis Otto, ed. Fluorescence methods and applications: Spectroscopy, imaging, and probes. Malden, Mass: Blackwell Pub., 2008.

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Kimmel, Jyrki. Modeling of optical waveguide biosensor structures. Espoo: Technical Research Centre of Finland, 1992.

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GFP whole cell microbial biosensors: Scale-up and scale-down effects on biopharmaceutical processes. New York, N.Y: ASME Press, 2013.

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R, Hansmann Douglas, ed. Proceedings of Fiber Optic Medical and Fluorescent Sensors and Applications, 23-24 January 1992, Los Angeles, California. Bellingham, Wash: SPIE, 1992.

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service), ScienceDirect (Online, ed. Single molecule tools: Super-resolution, particle tracking, multiparameter and force based methods. San Diego, CA: Academic Press/Elsevier, 2010.

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T, Papazoglou, Wagnières Georges A, Optical Society of America, Society of Photo-optical Instrumentation Engineers., and Deutsche Gesellschaft für Lasermedizin, eds. Diagnostic optical spectroscopy in biomedicine: 19-21 June 2001, Munich, Germany. Bellingham, Wash., USA: SPIE, 2001.

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Achilefu, Samuel. Molecular probes for biomedical applications II: 21-22 January 2008, San Jose, California, USA. Edited by SPIE (Society). Bellingham, Wash: SPIE, 2008.

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Achilefu, Samuel. Genetically engineered and optical probes for biomedical applications IV: 23-24 January 2007, San Jose, California, USA. Edited by Society of Photo-optical Instrumentation Engineers. Bellingham, Wash: SPIE, 2007.

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Book chapters on the topic "Fluorescent biosensor"

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Greenwald, Eric C., Renata K. Polanowska-Grabowska, and Jeffrey J. Saucerman. "Integrating Fluorescent Biosensor Data Using Computational Models." In Methods in Molecular Biology, 227–48. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-622-1_18.

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Yin, Kun. "A Fluorescent Biosensor for Copper Ion Detection." In Design of Novel Biosensors for Optical Sensing and Their Applications in Environmental Analysis, 13–24. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-6488-4_2.

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Hedgethorne, Katy, and Martin R. Webb. "Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA." In Single-Stranded DNA Binding Proteins, 219–33. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-032-8_17.

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Hofmann, Roseanne M., Graham J. Cotton, William Bornman, Emmanual Chang, and Tom W. Muir. "Fluorescent Biosensor for CrkII Phosphorylation by the Abl Tyrosine Kinase." In Peptides: The Wave of the Future, 992–93. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0464-0_463.

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Cohen, Michael S., Melissa L. Stewart, Richard H. Goodman, and Xiaolu A. Cambronne. "Methods for Using a Genetically Encoded Fluorescent Biosensor to Monitor Nuclear NAD+." In Methods in Molecular Biology, 391–414. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8588-3_26.

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Hung, Yin Pun, and Gary Yellen. "Live-Cell Imaging of Cytosolic NADH–NAD+ Redox State Using a Genetically Encoded Fluorescent Biosensor." In Methods in Molecular Biology, 83–95. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-622-1_7.

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Haugland, Richard P. "Fluorescent Labels." In Biosensors with Fiberoptics, 85–110. Totowa, NJ: Humana Press, 1991. http://dx.doi.org/10.1007/978-1-4612-0483-1_3.

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Yoon, Jeong-Yeol. "Fluorescence." In Introduction to Biosensors, 153–70. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-27413-3_9.

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Strianese, Maria, Maria Staiano, Giuseppe Ruggiero, Tullio Labella, Claudio Pellecchia, and Sabato D’Auria. "Fluorescence-Based Biosensors." In Methods in Molecular Biology, 193–216. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-806-1_9.

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Chatterjee, Shamba, and Sayanta Pal Chowdhury. "Development of a Multidrug Transporter Deleted Yeast-Based Highly Sensitive Fluorescent Biosensor to Determine the (Anti)Androgenic Endocrine Disruptors from Environment." In Advancements of Medical Electronics, 161–73. New Delhi: Springer India, 2015. http://dx.doi.org/10.1007/978-81-322-2256-9_15.

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Conference papers on the topic "Fluorescent biosensor"

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Gaidamauskaite, E., and R. Baronas. "Modelling A Peroxidase-Based Fluorescent Biosensor." In 22nd Conference on Modelling and Simulation. ECMS, 2008. http://dx.doi.org/10.7148/2008-0152.

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Byungchul Jang, Peiyan Cao, A. Chevalier, A. Ellington, and A. Hassibi. "A CMOS fluorescent-based biosensor microarray." In 2009 IEEE International Solid-State Circuits Conference (ISSCC 2009). IEEE, 2009. http://dx.doi.org/10.1109/isscc.2009.4977495.

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Su, Yu-Zheng, Min-Wei Hung, Wen-Hong Wu, Kuo-Cheng Huang, and Huihua Kenny Chiang. "Application of metal-enhanced fluorescence technology in evanescent wave fluorescent biosensor." In 2010 IEEE Instrumentation & Measurement Technology Conference Proceedings. IEEE, 2010. http://dx.doi.org/10.1109/imtc.2010.5488270.

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Gunda, Naga Siva Kumar, and Sushanta K. Mitra. "Microfluidic Based Biosensor for Detection of Cardiac Markers." In ASME 2013 Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/fedsm2013-16270.

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Myocardial Infarction (MI) occurs when the blood flow to the heart is blocked. It is major threat to human kind. Current laboratory and ELISA tests are expensive, time consuming, and are not very sensitive. Biosensors can play an important role in the diagnosis of MI without relying on hospital visits. Therefore, researchers are focusing to develop rapid, hand-held, inexpensive biosensors for detecting cardiac markers. In the present study, one of the cardiac markers (Troponin T) is detected using microfluidic based biosensor. Troponin T (cTnT) releases in to the blood serum within 4–6 h after minor heart attack and remains elevated for up to 2 weeks, which will help in diagnosing the heart condition. In this work, a microfluidic channel with an array of gold strips is considered for detecting and quantifying the Troponin T in an aqueous solution. Troponin T primary (capture) antibody is immobilized on gold strip using self assembled monolayer (SAM) consisting of a homogeneous mixture of oligo (ethylene glycol) (OEG)-terminated alkanethiolate and mercaptohexadecanoic acid (MHDA). Then, an aqueous solution containing Troponin T antigen is injected into the microchannel to facilitate antibody-antigen reaction to take place in less time. Later, FITC tagged Troponin T secondary (detection) antibody is dispensed in to the channel for quantification of Troponin T antigen. Using confocal fluorescent reader, the variation of fluorescent intensity across the microchannel is measured and quantified the concentration of Troponin T antigen with calibrated samples. Contact angle measurement system, Fourier Transform Infrared Spectroscopy and Ellipsometer are used to characterize the surface properties at each stage of biomolecule immobilization.
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Mujiono, Totok, and Tasripan Tasripan. "OPTICAL FLUORESCENT INSTRUMENTATION DEVELOPEMENT SUITABLE FOR CELL BASED BIOSENSOR." In Seminar Nasional Instrumentasi, Kontrol dan Otomasi 2018. Pusat Teknologi Instrumentasi dan Otomasi ITB, 2019. http://dx.doi.org/10.5614/sniko.2018.21.

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Horii, Kazuyoshi, Toshihito Kimura, Hisashi Ohtsuka, Noriyuki Kasagi, Tomoya Oohara, Tadahiro Matsuno, Masashi Hakamata, Akihiro Komatsu, and Tomonari Sendai. "Surface plasmon enhanced-field fluorescence biosensor for point-of-care testing using fluorescent nanoparticles." In SPIE BiOS, edited by Tuan Vo-Dinh and Joseph R. Lakowicz. SPIE, 2012. http://dx.doi.org/10.1117/12.908099.

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Kothe, J., A. Schröter, G. Gerlach, K. Zarschler, G. Rödel, D. Wersing, M. Mkandawire, and W. Pompe. "4.4 - Optical Biosensor Based on the Dependent Expression of Fluorescent Proteins." In SENSOR+TEST Conferences 2011. AMA Service GmbH, Von-Münchhausen-Str. 49, 31515 Wunstorf, Germany, 2011. http://dx.doi.org/10.5162/opto11/o4.4.

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Heller, Michael J., Dieter Dehlinger, Sadik Esener, and Benjamin Sullivan. "Electric Field Directed Fabrication of Biosensor Devices From Biomolecule Derivatized Nanoparticles." In ASME 2007 2nd Frontiers in Biomedical Devices Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/biomed2007-38093.

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An electronic microarray has been used to carry out directed self-assembly of higher order 3D structures from Biotin/Streptavidin and DNA derivatized nanoparticles. Structures with more than forty layers of alternating biotin and streptavidin and DNA nanoparticles were fabricated using a 400 site CMOS microarray system. In this process, reconfigurable electric fields produced by the microarray device have been used to rapidly transport, concentrate and accelerate the binding of 40 and 200 nanometer biotin, streptavidin, DNA and peroxidase derivatized nanoparticles to selected sites on the microarray. The nanoparticle layering process takes less than one minute per layer (10–20 seconds for addressing and binding nanoparticles, 40 seconds for washing). The nanoparticle addressing/binding process can be monitored by changes in fluorescence intensity as each nanoparticle layer is deposited. The final multilayered 3-D structures are about two microns in thickness and 50 microns in diameter. Work is now focused on assembling “micron size” biosensor devices from bio-molecule derivatized luminescent and fluorescent nanoparticles. The proposed structure for a nanolayered glucose sensor device includes a base layer of biotin/streptavidin nanoparticles, a layer of glucose oxidase derivatized nanoparticles, a layer of peroxidase derivatized nanoparticles, a layer of quantum dots, and a final layer of biotin/streptavidin nanoparticles. Such a device will serve as a prototype for a wide variety of applications which includes other biosensor devices, lab-on a-chip devices, in-vivo drug delivery systems and “micron size” dispersible bio/chem sensors for environmental, military and homeland security applications.
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Sukekawa, Yuji, Totok Mujiono, and Takamichi Nakamoto. "Two-Dimensional Digital Lock-In Circuit for Fluorescent Imaging of Odor Biosensor System." In 2017 New Generation of CAS (NGCAS). IEEE, 2017. http://dx.doi.org/10.1109/ngcas.2017.76.

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Cady, Nathaniel, Gabriel Zenteno, Benjamin Taubner, Eunice Chou, Arturo Pilar, Ernest Guignon, William Page, and Yi-Pin Lin. "Grating coupled-surface plasmon resonance and fluorescent plasmonics biosensor for diagnosis of Lyme disease." In Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIX, edited by Augustus W. Fountain, Jason A. Guicheteau, and Chris R. Howle. SPIE, 2018. http://dx.doi.org/10.1117/12.2303798.

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Reports on the topic "Fluorescent biosensor"

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Darrow, C., T. Huser, C. Campos, M. Yan, S. Lane, and R. Balhorn. Single Fluorescent Molecule Confocal Microscopy: A New Tool for Molecular Biology Research and Biosensor Development. Office of Scientific and Technical Information (OSTI), March 2000. http://dx.doi.org/10.2172/792442.

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