Relevant bibliographies by topics / Fluorescent Bioimaging

Academic literature on the topic 'Fluorescent Bioimaging'

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Published: 7 September 2023

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Journal articles on the topic "Fluorescent Bioimaging"

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ZHU, MING-QIANG, GUO-FENG ZHANG, CHONG LI, YA-JING LI, MATTHEW P. ALDRED, and ALEXANDER D. Q. LI. "PHOTOSWITCHABLE NANOFLUOROPHORES FOR INNOVATIVE BIOIMAGING." Journal of Innovative Optical Health Sciences 04, no. 04 (October 2011): 395–408. http://dx.doi.org/10.1142/s1793545811001423.

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Photosensitive fluorescent probes have become powerful tools in chemical biology and molecular biophysics, which are used to investigate cellular processes with high temporal and spatial resolution. Accordingly, photosensitive fluorescent probes, including photoactivatable, photoconvertible, and photoswitchable fluorophores, have been extensively developed during the past decade. The photoswitchable fluorophores have received much attention because they highlight cellular events clearly. This minireview summarizes recent advances of using reversibly photoswitchable fluorophores and their applications in innovative bioimaging. Photoswitchable fluorophores include photoswitchable fluorescent proteins, photoswitchable fluorescent organic molecules (dyes), and photoswitchable fluorescent nanoparticles. Several strategies have been developed to synthesize photoswitchable fluorophores, including engineering combination proteins, chemical synthesis, polymerization, and self-assembly. Here we concentrate on polymer nanoparticles with optically switchable emission properties: either fluorescence on/off or dual-alternating-color fluorescence photoswitching. The essential mechanisms of fluorescence photoswitching enable different types of photoswitchable fluorophores to change emission intensity or wavelength (color) and thus validating the basis of the fluorescence on/off or dual-color photoswitching design. Generally the possible applications of any fluorophores are to label biological targets, followed by specific imaging. The newly developed photoswitchable fluorophores enable super-resolution fluorescence imaging because of their photosensitive emission. Finally, we summarize the important area regarding future research and development on photoswitchable fluorescent nanoparticles.
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Gedara, Sriyani Menike Korale, Zi-You Ding, Iresha Lakmali Balasooriya, Yingchao Han, and Merita Nirmali Wickramaratne. "Hydrothermal Synthesis and In Vivo Fluorescent Bioimaging Application of Eu3+/Gd3+ Co-Doped Fluoroapatite Nanocrystals." Journal of Functional Biomaterials 13, no. 3 (July 29, 2022): 108. http://dx.doi.org/10.3390/jfb13030108.

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In this study, Eu3+/Gd3+ co-doped fluoroapatitååe (Eu/Gd:FAP) nanocrystals were synthesized by the hydrothermal method as a fluorescent bioimaging agent. The phase composition, morphology, fluorescence, and biosafety of the resulting samples were characterized. Moreover, the in vivo fluorescent bioimaging application of Eu/Gd:FAP nanocrystals was evaluated in mice with subcutaneously transplanted tumors. The results showed that the Eu/Gd:FAP nanocrystals were short rod-like particles with a size of 59.27 ± 13.34 nm × 18.69 ± 3.32 nm. With an increasing F substitution content, the Eu/Gd:FAP nanocrystals displayed a decreased size and enhanced fluorescence emission. Eu/Gd:FAP nanocrystals did not show hemolysis and cytotoxicity, indicating good biocompatibility. In vivo fluorescent bioimaging study demonstrated that Eu/Gd:FAP nanocrystals could be used as a bioimaging agent and displayed stable fluorescence emitting in tumors, indicating an accumulation in tumor tissue due to the passive targeting ability. In addition, any adverse effects of Eu/Gd:FAP nanocrystals on major organs were not observed. This study shows that biocompatible rare earth co-doped FAP nanocrystals have the potential to be used as a bioimaging agent in vivo.
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Wang, Xinyue, Dandan Sang, Liangrui Zou, Shunhao Ge, Yu Yao, Jianchao Fan, and Qinglin Wang. "Multiple Bioimaging Applications Based on the Excellent Properties of Nanodiamond: A Review." Molecules 28, no. 10 (May 12, 2023): 4063. http://dx.doi.org/10.3390/molecules28104063.

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Nanodiamonds (NDs) are emerging as a promising candidate for multimodal bioimaging on account of their optical and spectroscopic properties. NDs are extensively utilized for bioimaging probes due to their defects and admixtures in their crystal lattice. There are many optically active defects presented in NDs called color centers, which are highly photostable, extremely sensitive to bioimaging, and capable of electron leap in the forbidden band; further, they absorb or emit light when leaping, enabling the nanodiamond to fluoresce. Fluorescent imaging plays a significant role in bioscience research, but traditional fluorescent dyes have some drawbacks in physical, optical and toxicity aspects. As a novel fluorescent labeling tool, NDs have become the focus of research in the field of biomarkers in recent years because of their various irreplaceable advantages. This review primarily focuses on the recent application progress of nanodiamonds in the field of bioimaging. In this paper, we will summarize the progress of ND research from the following aspects (including fluorescence imaging, Raman imaging, X-ray imaging, magnetic modulation fluorescence imaging, magnetic resonance imaging, cathodoluminescence imaging, and optical coherence tomography imaging) and expect to supply an outlook contribution for future nanodiamond exploration in bioimaging.
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Yang, Yufei, Fucheng Gao, Yandong Wang, Hui Li, Jie Zhang, Zhiwei Sun, and Yanyan Jiang. "Fluorescent Organic Small Molecule Probes for Bioimaging and Detection Applications." Molecules 27, no. 23 (December 1, 2022): 8421. http://dx.doi.org/10.3390/molecules27238421.

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The activity levels of key substances (metal ions, reactive oxygen species, reactive nitrogen, biological small molecules, etc.) in organisms are closely related to intracellular redox reactions, disease occurrence and treatment, as well as drug absorption and distribution. Fluorescence imaging technology provides a visual tool for medicine, showing great potential in the fields of molecular biology, cellular immunology and oncology. In recent years, organic fluorescent probes have attracted much attention in the bioanalytical field. Among various organic fluorescent probes, fluorescent organic small molecule probes (FOSMPs) have become a research hotspot due to their excellent physicochemical properties, such as good photostability, high spatial and temporal resolution, as well as excellent biocompatibility. FOSMPs have proved to be suitable for in vivo bioimaging and detection. On the basis of the introduction of several primary fluorescence mechanisms, the latest progress of FOSMPs in the applications of bioimaging and detection is comprehensively reviewed. Following this, the preparation and application of fluorescent organic nanoparticles (FONPs) that are designed with FOSMPs as fluorophores are overviewed. Additionally, the prospects of FOSMPs in bioimaging and detection are discussed.
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Luo, Xiaofeng, Jiaqi Meng, Baolin Li, Aidong Peng, and Zhiyuan Tian. "Development of fluorescent nanoparticles with aggregation-induced delayed fluorescence features, improved brightness and photostability for living cells imaging." New Journal of Chemistry 43, no. 27 (2019): 10735–43. http://dx.doi.org/10.1039/c9nj01945f.

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Lin, Zhong, Hong Wang, Maolin Yu, Xiang Guo, Chonghua Zhang, Haitao Deng, Peisheng Zhang, et al. "Photoswitchable ultrahigh-brightness red fluorescent polymeric nanoparticles for information encryption, anti-counterfeiting and bioimaging." Journal of Materials Chemistry C 7, no. 37 (2019): 11515–21. http://dx.doi.org/10.1039/c9tc04054d.

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Péresse, Tiphaine, and Arnaud Gautier. "Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging." International Journal of Molecular Sciences 20, no. 24 (December 5, 2019): 6142. http://dx.doi.org/10.3390/ijms20246142.

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Our ability to observe biochemical events with high spatial and temporal resolution is essential for understanding the functioning of living systems. Intrinsically fluorescent proteins such as the green fluorescent protein (GFP) have revolutionized the way biologists study cells and organisms. The fluorescence toolbox has been recently extended with new fluorescent reporters composed of a genetically encoded tag that binds endogenously present or exogenously applied fluorogenic chromophores (so-called fluorogens) and activates their fluorescence. This review presents the toolbox of fluorogen-based reporters and biosensors available to biologists. Various applications are detailed to illustrate the possible uses and opportunities offered by this new generation of fluorescent probes and sensors for advanced bioimaging.
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Song, Chenxi, Shubiao Zhang, Quan Zhou, Hua Hai, Defeng Zhao, and Yunze Hui. "Upconversion nanoparticles for bioimaging." Nanotechnology Reviews 6, no. 2 (April 1, 2017): 233–42. http://dx.doi.org/10.1515/ntrev-2016-0043.

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AbstractFluorescent labeling is a widely used indispensable tool in biology. Conventional downconversion fluorescence labels with ultraviolet or short-wavelength excitation suffer from autofluorescence, low signal-to-noise ratio, and incident photo damage to living organisms. However, upconverting fluorescent nanoparticles emit detectable photons of higher energy in the near-infrared (NIR) or visible range upon irradiation with a NIR light in a process termed upconversion. They overcome some of the disadvantages faced by conventional downconversion labels with the advantages including very low autofluorescence, absence of photo damage to living organisms, high detection sensitivity, and high depth of light penetration, thus making them an ideal fluorescent label for bioimaging. The present review focuses on the features of upconversion nanoparticles, the applications of upconversion nanoparticles in bioimaging, and the bioimaging equipments and methods and discusses the obstacles and development trend of upconversion nanoparticles in bioimaging; we hope this work will provide insights into the study of relevant fields. Upconversion nanoparticles have special photoluminescent properties. Substantial advancements have been made in the field of upconversion nanoparticles for bioimaging. A large number of modifications of upconversion nanoparticles are studied to make them more hydrophilic and biocompatible. At the same time, the safety and toxicity of nanoparticles have caused wide public concern.
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Terai, Takuya, and Tetsuo Nagano. "Fluorescent probes for bioimaging applications." Current Opinion in Chemical Biology 12, no. 5 (October 2008): 515–21. http://dx.doi.org/10.1016/j.cbpa.2008.08.007.

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Pu, Kan-Yi, and Bin Liu. "Fluorescent Conjugated Polyelectrolytes for Bioimaging." Advanced Functional Materials 21, no. 18 (August 15, 2011): 3408–23. http://dx.doi.org/10.1002/adfm.201101153.

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Dissertations / Theses on the topic "Fluorescent Bioimaging"

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Choi, Angela On Ki. "Fluorescent nanocrystals for bioimaging." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=114126.

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Optical imaging based on fluorescence has yet to be introduced as a clinical diagnostic tool due to the lack of reliable, photostable, and highly luminescent fluorophores. Fluorescent nanocrystals, or quantum dots (QDs), are promising alternatives to organic dyes, since QDs are small in size, resistant to photo-bleaching, and have excellent and appropriate optical properties. The main objective of this work is to use QDs for real-time imaging in live animals. Widespread use of QDs in biology is currently limited due to their questionable biocompatibility, and to the fact that some nanocrystals contain heavy metals, which are potentially hazardous, in their cores. In the present studies the mechanisms underlying the toxicity of cadmium telluride QDs was investigated in several stable cell lines. After long-term exposure to QDs, significant morphological and functional changes were observed at the cellular and subcellular levels. We showed that QD-induced toxicity includes the production of reactive oxygen species, peroxidation of membrane lipids, impairment of mitochondrial function, and changes in the genome and epigenome. Understanding how toxic QDs cause damage to the cells is a first step for i) the establishment of protocols to evaluate the safety of other nanomaterials, and ii) the development of new or improved nanocrystals that are non-toxic. We showed that modifications on QD surfaces with small drug molecules (e.g. N-acetylcysteine) or synthetic polymers can significantly decrease their toxicity, and in some cases, even render the QDs non-toxic. Utilizing a non-invasive route (i.e. intranasal) to deliver nano-probes and nano-therapeutics to the brain, we demonstrated the use of near-infrared fluorescence of non-toxic QDs to image cerebral microlesions in live animals. Repeated imaging in vivo allowed for the live monitoring of lesion size in animals; a reduction of lesion size is a measure of the effectiveness of nano-therapeutic interventions. Animals treated with micelle-incorporated nimodipine or minocycline had significantly smaller lesion volumes, and displayed better recovery of motor function. Quantitative evaluation and volume calculations were possible since the QD signal was isolated from autofluorescence and background after fluorescence lifetime gating. Taken together, the results from this work contribute to the development of QDs and fluorescence technology for biomedical imaging in two main ways: 1) by presenting in vitro measures as the first step in the evaluation of nanomaterial safety. 2) by demonstrating the advantages of using near-infrared QDs for non-invasive lifetime imaging in animals with unilateral cortical ischemic microlesions and for the determination of the spatio-temporal reduction of lesions upon nano-therapeutic interventions. These findings support the use of carefully designed and rigorously tested fluorescent QDs for lifetime optical imaging of the brain in experimental animals, and eventually extending to clinical studies.
L'imagerie par fluorescence reste à introduire dans les cabinets médicaux en raison du manque de fluorophores photo-stables, à haute intensité lumineuse, disponibles sur le marché. Les nanocristaux fluorescents ou boîtes quantiques (BQ), représentent une alternative intéressante par rapport aux teintures organiques car les BQ sont très petits, résistants au photoblanchiment et ont d'excellentes propriétés optiques. L'objectif principal de cette étude est d'utiliser les BQ pour une imagerie en temps réel sur les animaux vivants. L'usage étendu des BQ en biologie est limité en raison de leur biocompatibilité discutable et également en raison du fait que quelques nanocristaux sont composés en partie de métaux lourds. Dans cette étude, les mécanismes cellulaires impliquant la toxicité des BQ de cadmium telluride sont examinés. Après une exposition prolongée aux BQ, des modifications morphologiques et fonctionnelles significatives ont été observées à l'échelle cellulaire et infracellulaire. Nous démontrons que la toxicité induite par les BQ peut entrainer la production d'espèces réactives de l'oxygène, la peroxydation des lipides de la membrane biologique, l'altération du fonctionnement mitochondrial mais aussi des changements du génome et de l'épigénome. Comprendre comment les BQ toxiques endommagent les cellules est un premier pas dans l'établissement de protocoles d'évaluation de la sécurité des nanomatériaux et dans le développement de nouveau nanocristaux non-toxiques. Nous démontrons que la modification de la surface des BQ grâce à des médicaments (ex : N-acetylcysteine) ou des polymères synthétiques peut grandement diminuer leur toxicité, et dans quelques cas, peut aussi rendre les BQ non-toxiques. En utilisant de tel BQ non-toxiques, nous effectuons une démonstration de l'utilisation de la fluorescence infrarouge proche pour effectuer des clichés en temps réel de microlésions cérébrales sur des animaux vivants, à l'aide de méthodes non effractives (ex : voie intra-nasale) pour insérer des nano-sondes ou administrer des nano-thérapies au niveau du cerveau. Des imageries répétées permettent de surveiller la taille des lésions sur les animaux, et prouvent l'efficacité des nano-thérapies dans la prévention de l'expansion de la lésion. Les animaux traités par micelles chargées de nimodipine ou de minocycline ont des lésions moins volumineuses et une meilleure récupération de la fonction motrice. Une évaluation quantitative et un calcul de volume ont été possibles car le signal BQ était séparé de l'autofluorescence tissulaire grâce à de la synchronisation d'image fondé sur la durée de vie fluorescence. L'ensemble des résultats de ces études contribue au développement des BQ et des technologies par fluorescence en imagerie biomédicale, et ceci de deux façons : 1) en présentant des résultats in vitro qui constituent une première étape dans l'évaluation de la sécurité des nanomatériaux. 2) en démontrant des avantages de l'utilisation les BQ infrarouges proches pour l'imagerie non effractives sur les animaux vivants avec des lésions cérébrales et pour la détermination de la réduction des lésions après des nano-thérapies. Ces constatations appuient l'utilisation des BQ fluorescentes créés avec soin et ayant subi des essais précliniques rigoureux pour l'imagerie encéphalique in vivo et s'étendant finalement aux études cliniques.
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Si, Yang. "Fluorescent Nanomaterials for Bioimaging and Biosensing : Application on E.coli Bacteria." Thesis, Cachan, Ecole normale supérieure, 2015. http://www.theses.fr/2015DENS0038/document.

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Les bactéries sont les organismes les plus abondants dans le monde. Des études sur les bactéries peuvent être bénéfiques pour la recherche médicale, la qualité des ressources en eau et l'industrie alimentaire. La détection et le marquage fluorescent est une des méthodes les plus utilisées pour des objectifs bioanalytiques. Dans la recherche de marqueurs luminescents et stables, des nouvelles nanoparticules fluorescentes et auto-stabilisées à base de polymères (FNPs, 60 nm) et des chaînes de polymères fluorescents (FPCs, 5nm) ont été développées. Dans un premier chapitre, une méthodologie pour insérer ces FNPs dans la bactérie E.coli a été développée. Pour contrôler si les FNPs sont en effet internalisé, nous avons développé un protocole basé sur l'extinction de luminescence des FNPs par le bleu de méthylène. Dans un second chapitre, les biotines conjuguées de FNPs peuvent être utilisées pour étudier les protéines membranaires spécifiques. En utilisant un lien streptavidine-biotine, un "sandwich" est formé pour construire un pont entre des particules, des anticorps spécifiques et des bactéries. Les images de fluorescence SPR et les images SEM ont démontré l'interaction de la biotine conjuguée de FNPs avec la bactérie E.coli. Dans un troisième chapitre, les chaînes de polymères fluorescents de couleur verte (GFPCs) peuvent facilement entrer dans des bactéries E.coli. Les GFPCs peuvent marquer le cyctoplasme mais pas l'ADN. Les chaînes de polymères fluorescents de couleur rouge (RFPCs) peuvent marquer facilement et efficacement la membrane de bactérie E.coli. Les deux FPCs sont extrêmement brillantes et non toxiques, les chaînes sont solubles dans l'eau. Ce sont de nouveaux matériaux fluorescents pour le marquage interne et externe des bactéries. Dans le dernier chapitre, il est démontré que les FANPs sont sensibles au pH et peuvent être utilisées pour mesurer la croissance de la bactérie E.coli. Les nano-objets détectent rapidement et précisément la croissance des cellules. En effet, leur fluorescence est sensible au changement de pH résultant du métabolisme cellulaire. De plus, ces particules permettent une surveillance en continu d'un grand nombre d'échantillons pour des applications de criblage à haut débit. Les nanomatériaux présentés dans ce manuscrit sont des outils prometteurs pour les applications en biocapteurs et bioimagerie en raison de leur luminosité/brillance et photostabilité élevées ainsi que les possibilités de post-fonctionnalisation
Bacteria are the most abundant organisms in the world. Investigations and studies on bacteria can be beneficial to medical research, water resources research and food industry. Fluorescent sensing and labeling are commonly used for bioanalytical purposes. In the quest for very bright and stable labels, novel polymer-based, self-stabilized, fluorescent nanoparticles (FNPs, 60 nm) and fluorescent polymer chains (FPCs, 5 nm) have been developed. In the first part, a methodology to insert these FNPs into E.coli bacteria was developed. To control if the FNPs are indeed internalized, we developed a protocol based upon FNPs luminescence quenching by methylene blue. In the second part, a "sandwich" system is built. By using a streptavidin-biotin link, a bridge between particles (FNP), specific antibodies and bacteria is built. SPR, fluorescent images and SEM images demonstrated the interaction of biotin conjugated FNPs with E.coli bacteria. In the third part, interactions of fluorescent polymer chains with bacteria are investigated. Green fluorescent polymer chains (GFPCs) can easily enter into E.coli bacteria. GFPCs can label the cytoplasm but not the DNA. Red fluorescent polymer chains (RFPCs) can label the membrane of E.coli bacteria easily and efficiently. Both FPCs are highly water-soluble, bright and non-toxic, they are novel fluorescent labels for internal and external biological labeling of bacteria. In the last part, it is demonstrated that pH sensitive FANPs can be used to measure the growth of E.coli. They detect rapidly and accurately bacterial growth by signaling the change of pH resulting from cellular metabolism. Moreover, these particles allow for continuous monitoring a large number of samples for high-throughput screening applications. The studied fluorescent nanomaterials are promising tools for biosensing and bioimaging applications due to their brightness, high photostability and rich functionalisation ability
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Kong, Yifei. "Multifunctional fluorescent nanoparticle-bioconjugates : preparation, characterisation and bioimaging applications." Thesis, University of Leeds, 2015. http://etheses.whiterose.ac.uk/12252/.

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Trofymchuk, Kateryna. "Controlled switching of fluorescent organic nanoparticles through energy transfer for bioimaging applications." Thesis, Strasbourg, 2016. http://www.theses.fr/2016STRAJ121/document.

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Les performances des techniques de bioimagerie et de biodétection peuvent être améliorées grâce aux nanoparticules fluorescentes (NPs) permettant un transfert d’énergie résonante de type Förster (FRET) efficace. Le but de mon projet de thèse est le développement de NPs polymériques brillantes et ultrastables encapsulant des fluorophores, capables de produire un FRET au-delà du rayon de Förster. Il a été montré que les groupements encombrés sont essentiels pour minimiser l’auto-extinction et le blanchiment des fluorophores encapsulés. Par ailleurs, la matrice polymérique joue un rôle crucial dans le contrôle de l’effet collaboratif entre fluorophores du au transfert d’énergie d’excitation. Puis, en utilisant cet effet collaboratif entre fluorophores, nous avons conçu des NPs présentant une photocommutation efficace, ainsi qu'un phénomène de "light harvesting" très important. Enfin, de très petites NPs avec un FRET efficace à leur surface ont été élaborées et appliquées pour la détection ultra-sensible de protéines. Les résultats obtenus fournissent de nouvelles perspectives dans le développement des nanoparticules brillantes avec un transfert d'énergie efficace, ainsi que des nano-sondes pour la détection de molécules uniques
Performance of biosensing and bioimaging techniques can be improved by fluorescent nanoparticles (NPs) capable of efficient Förster resonance energy transfer (FRET). The aim of my PhD project is to develop bright and photostable dye-loaded polymer NPs capable to undergo efficient FRET beyond the Förster radius. We showed that bulky groups are essential for minimizing self-quenching and bleaching of encapsulated dyes. Moreover, polymer matrix plays a crucial role in controlling the inter-fluorophore communication by excitation energy transfer. Then, by exploiting communication of dyes, we designed NPs exhibiting efficient photoswitching as well as giant light-harvesting. Finally, very small NPs with efficient FRET to their surface were developed and applied for ultra-sensitive molecule detection of proteins. The obtained results provide new insights in the development of bright nanoparticles with efficient energy transfer as well as nano-probes for single-molecule detection
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Mastrodonato, Cristiano Matteo. "Elaboration of fluorescent molecular probes and molecular-based nanoparticles for bioimaging purposes." Thesis, Bordeaux, 2017. http://www.theses.fr/2017BORD0652/document.

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Les techniques de fluorescence sont des outils de choix pour l’étude et la compréhension fine des processus biologiques. Ceci requiert toutefois l’utilisation de sondes fluorescentes parfaitement adaptées au but visé et répondant aux différentes exigences requises pour l’application visée. Dans ce cadre, nous nous sommes plus particulièrement intéressés à l’élaboration de sondes biphotoniques de pH adaptées à une mesure très sensible de faibles variations de pH autour du pH neutre. Les variations et gradients de pH sont en effet impliqués dans un certain nombre de processus biologiques importants et peuvent être associées à des dysfonctionnements liés à certaines maladies. Dans ce cadre, nous avons développé de nouvelles sondes fluorescentes de pH fluorescentes présentant à la fois un comportement ratiométrique, une forte sensibilité autour du pH neutre et facilement excitables dans le proche IR par absorption à deux photons. Ces sondes de structure quadrupolaire et bolamamphiphile permettent ainsi la détection ratiométrique du pH dans des environnements biologiques au moyen d'une excitation biphotonique dans le proche IR. En parallèle, nous nous sommes intéressés à l’élaboration de nanoparticules hyperbrillantes dédiées à l’imagerie biologique par microscopie de fluorescence induite par excitation à deux photons. Nous nous sommes plus particulièrement attachées au design de nanoparticules organiques fluorescentes constituées de molécules organiques de bas poids moléculaire (FONs). Cette approche offre en effet une grande flexibilité et la possibilité d’accéder à des nanosondes ayant des brillances comparables aux très populaires quantum dots mais moins toxiques et plus facilement dégradables. L’ingénierie moléculaire des fluorophores utilisés pour la préparation des FON est cruciale puisqu’elle influence fortement à la fois les propriétés photophysiques (brillance, couleur…) et leur propriétés physico-chimiques (stabilité chimique et structurale, stabilité colloïdale). Dans ce contexte, une librairie de nouveaux chromophores dipolaires a été synthétisée et utilisées pour la préparation de FON par la méthode de nano-précipitation. Leurs propriétés ont été étudiées afin de déterminer la relation entre la structure du chromophore et les propriétés globales des nanoparticules constituées de ces colorants. Ce travail a permis d’identifier les paramètres structuraux permettant d’accéder à des nanoparticules présentant à la fois une brillance exceptionnelle, une émission modulable du vert au rouge et proche IR et une remarquable stabilité colloïdale. Ces nanoparticules présentent des potentialités majeures pour l’imagerie in vivo par excitation et détection dans le proche IR
Fluorescence-based techniques are popular tools for the study and understanding of biological processes. This has prompted continuous research aimed at the development of a wide range of fluorescent probes specifically designed for specific applications. Among them, fluorescent pH probes are of much interest as pH variations or gradients are involved in many biological events and anomalous alterations are often related to the onset of dysfunctions and diseases. In this framework we have developed a series of promising two-photon pH fluorescent molecular probes. These quadrupolar bolaamphiphilic probes are of great interest, as they combine a steep pH dependence of their optical properties close to neutral pH, ratiometric behavior and large response to two-photon (2P) excitation in the NIR region. As such they offer much promise for ratiometric detection of the pH in biological environments and in situ monitoring of acidification. In parallel, we have been interest in the design of ultrabright nanoparticles for bioimaging purpose (in particular highly sensitive optical imaging). We chose to focus on Fluorescent Organic Nanoparticles made of organic molecules with low molecular weight (FONs) as they offer a flexible route and promising alternatives to toxic quantum dots. In this case the design of the dye used as building blocks of the FONs is of crucial importance and strongly influence the chemical and physical properties of the nanoparticles generated, such as their one and two-photon brightness and both their structural and colloidal stability. In that context a library of novel dipolar chromophores have been synthesized and used to prepare FONs using the nanoprecipitation method. Their properties were thoroughly investigated in order to determine the relationship between the molecular design of the isolated dye and the overall properties of the nanoparticles made of these dyes. As a result, Hyperbright FONs emitting in the green to NIR region and combining giant brightness and remarkable stability have been achieved. They offer major promise for bioimaging based on both excitation and detection in the NIR region
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Ardizzone, Antonio. "New fluorescent nanovesicles, by self-assembly of organic fluorophores, sterols and surfactants, as probes for bioimaging." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/403924.

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El estudio de nuevas nanopartículas orgánicas fluorescentes (FONs) que puedan superar los límites de las comunes sondas fluorescentes como fluoróforos moleculares, proteínas fluorescentes y nanopartículas inorgánicas es un tema de gran interés para los científicos de materiales que desarrollan nuevas sondas para microscopía de fluorescencia y teranóstica. En los últimos años, se han desarrollado nuevas nanovesículas no liposómicas, basadas en el autoensamblaje de tensioactivos y esteroles, denominados Quatsomes (QSs), que constituyen unos prometedores vehículos de fármacos multifuncionales. Dentro de este escenario, el objetivo principal de esta Tesis (realizada en el marco del proyecto Marie Skłodowska-Curie ITN “Nano2Fun”) es explorar la posibilidad de utilizar los Quatsomes como vehículos para la nano-estructuración en medios acuosos de varias moléculas fluorescentes, independientemente de sus propiedades físico-químicas y ópticas, con el fin de obtener nuevas nanopartículas orgánicas fluorescentes (FONs) con elevada estabilidad coloidal y características fluorescentes optimales, especialmente en relación al brillo. El auto-ensamblaje de fluoróforos orgánicos moleculares, esteroles y tensioactivos de amonio cuaternario en Quatsomes fluorescentes se logró mediante el método DELOS-SUSP, un proceso a base de CO2 comprimido, que garantiza una composición de membrana y una disposición supramolecular altamente homogéneas y, como consecuencia, nanovesículas con elevadas propiedades ópticas. Se han explorado diferentes estrategias para la nano-estructuración en medios acuosos, por medio de QSs, de moléculas fluorescentes con diferentes propiedades fisicoquímicas, incluyendo aquellas solubles y no solubles en agua, analizando el impacto de la nano-estructuración sobre las propiedades ópticas de las FONs obtenidas. De esta manera, los fluoroforos aniónicos solubles en agua, como la fluoresceína, fueron nano-estructurados encima de los QSs. Por otra parte, los fluoróforos lipófilos y no solubles en agua, modificados con largas cadenas alquílicas pueden incorporarse de forma estable en la membrana de los QSs, como se muestra en el caso de varias familias de colorantes, por ejemplo, las cianinas, los diketopirrolopirroles (DPP) y los derivados del fluorene. Los QSs fluorescentes mostraron una estabilidad óptica coloidal excepcional (hasta varios meses), un alto grado de homogeneidad estructural y altas eficiencias de fluorescencia, mostrando mejores prestaciones en comparación con otras nanoestructuras de los mismos fluoroforos. Además, con el objetivo de obtener nanopartículas multicolores, los Quatsomes permitieron cargar simultáneamente diferentes fluoroforos dentro de sus membranas, mostrando un mecanismo de transferencia de energía de resonancia de fluorescencia (FRET) altamente eficiente, una interesante herramienta para monitorear la integridad del carrier durante la administración del fármaco y para la adquisición de imágenes multicolores. En conclusión, los Quatsomes fluorescentes se probaron como nano-sondas para la obtención de imágenes de células in vitro. Se ha demostrado que los Quatsomes que incorporan derivados del fluorene (denominados LysoQS) constituyen una sonda lisosómica altamente específica, ideal para la adquisición de imágenes en tiempos largos. Además, los Quatsomes cargados de cianinas se utilizaron como sondas para técnicas de microscopía superresolución (STORM), que permitió la visualización y resolución de Quatsomes individuales tras la internalización en las células. Los resultados de esta tesis muestran que los Quatsomes fluorescentes, gracias a las ventajas ofrecidas en comparación con otros marcadores fluorescentes comúnmente empleados, son unas nanosondas altamente prometedoras, con posibles aplicaciones futuras en bioimagen, teragnostica y, en general, nanomedicina.
Finding new fluorescent organic nanoparticles (FONs) with the potential to overcome the limits of common fluorescent probes as molecular fluorophores, fluorescent proteins and inorganic nanoparticles is a subject of strong interest for materials scientists developing new probes for fluorescence microscopy and theranostics. In the recent years, innovative non-liposomal nanovesicles, based on the self-assembly of quaternary ammonium surfactants and sterols, named Quatsomes (QSs), have been developed as promising candidates for applications as multifunctional drug carriers. Within this scenario, the main objective of this Thesis (conducted in the framework of the Marie Skłodowska-Curie ITN “Nano2Fun”) is to explore the possibility of using Quatsomes as a vehicle for nanostructuring in aqueous media several dye molecules, irrespective of their physicochemical and optical properties, in order to obtain new fluorescent organic nanoparticles (FONs) with superior colloidal stability and enhanced fluorescent features, especially with high brightness, in relation to single molecule flurofores and other type of FONS. The self-assembly of molecular organic fluorophores, sterols and quaternary ammonium surfactants into fluorescent Quatsomes was achieved by the DELOS-SUSP method, a compressed CO2 –based process which guarantees a highly homogeneous membrane composition and supramolecular arrangement, which have impact on the optical properties of the obtained FONs. Different strategies have been explored to nanostructurate in aqueous media, by mean of QSs, molecular dyes with different physicochemical properties, including those water- and non-water soluble, analyzing the impact of their nanostructuration on the optical properties of the obtained FONs. Thus, anionic water-soluble dyes, such as fluorescein, were nanostructured over QSs surface, taking advantage of anionic/cationic interaction among dye and vesicles surface. On the other hand, lipophilic and non-water soluble dyes modified with long alkyl chains can be stably incorporated into QSs membrane, as shown in the case of several dyes families, including cyanine, diketopyroolopyrrole (DPPs) and fluorene derivatives. The fluorescent QSs showed superior colloidal and optical stability (up to several months), a high degree of structural homogeneity and high fluorescence performances, overcoming those of other nanostructures of the same dyes. Furthermore, aiming to obtain multicolor nanoparticles, Quatsomes allowed the simultaneous loading within their membrane of different dyes, which showed a highly efficient fluorescence resonance energy transfer (FRET) mechanism, an interesting tool for monitoring the carrier integrity during the drug delivery and for multiplexed imaging applications. Finally, fluorescent Quatsomes were tested as nanoprobes for in vitro cells imaging. It has been demonstrated that fluorene-based Quatsomes (named LysoQS) constitute a strongly specific lysosomal probe ideal for long-term imaging. Furthermore, cyanines-loaded Quatsomes were used as probes for super-resolution microscopy technique (STORM) which allowed visualizing and resolving single Quatsomes structures upon internalization in cells. The results of this Thesis showed that fluorescent Quatsomes, thanks to the advantages offered in comparison with other commonly employed fluorescent labels, constitute a promising fluorescent nanoprobes with possible future applications in bioimaging, theranostics and, generally, nanomedicine.
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Nilsson, Ruben. "Optical properties of fluorescent quantum dots for super-resolution bioimaging." Thesis, KTH, Tillämpad fysik, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-169624.

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Lesani, Pooria. "Novel Carbon Dot-Based Fluorescent Nanomaterials for Biosensing and Bioimaging." Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/27346.

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Carbon dots (CDs)-based nanoparticles have been extensively explored for biological applications in sensing and bioimaging. However, the major translational barriers to CDs for imaging and sensing applications include optimal synthetic strategies to obtain monodisperse CDs with tunable structural, electronic, and optical properties in order to achieve high-resolution deep-tissue imaging, intracellular detection, and sensing of metal ions with high sensitivity down to nanomolar levels. In this thesis we have presented the synthesis and development of a series of novel carbon dot based nanoprobes with unique photophysical and biological properties for bioimaging and biosensing applications. These properties include water dispersibility, superior photostability and thermal stability, high quantum yield, excellent two-photon excitability, ease in surface functionalization, rapid cellular uptake, good biocompatibility, rapid detection of targeted molecules with a low detection limit, and high-resolution bioimaging capability. The CD-based probes developed in this study were used for two-photon intensity-based and ratiometric exogenous and endogenous ferric ions sensing in living cells, single- and two-photon deep tissue imaging in synthetic scaffold and complex biological tissue, and two-photon ratiometric real-time intracellular pH monitoring in 3D environment. Furthermore, the influence of CDs synthetic and post-synthetic parameters on parameters on photophysical properties and biological behavior of CDs were comprehensively investigated.
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Kilic, Nüzhet Inci. "Graphene Quantum Dots as Fluorescent and Passivation Agents for Multimodal Bioimaging." Thesis, KTH, Tillämpad fysik, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-298302.

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Zero-dimensional graphene (carbon) quantum dots have been drawing attention in bio-related applications since their discovery, especially for their optical properties, chemical stability, and easily modifiable surface.  This thesis focuses on the green synthesis of nitrogen-doped graphene quantum dots (GQDs) for dual-mode bioimaging with X-ray fluorescence (XRF) and optical fluorescence. Both conventional and microwave- (MW-)assisted solvothermal methods were followed to investigate the precursors’ effect on the synthesized GQDs. The MW-assisted method permitted the synthesis of uniform GQDs with an excitation-independent behavior, due to highly controllable reaction conditions. It was demonstrated that the molecular structure of the precursors influenced the optical fluorescence properties of the GQDs. Thus, both blue- (BQDs) and red-emitting (RQDs) GQDs were obtained by selecting specific precursors, leading to emission maxima at 438 and 605 nm under the excitation wavelengths of 390 and 585 nm, respectively.  Amine-functionalized Rh nanoparticles (NPs) were chosen as the X-ray fluorescence (XRF) active core, synthesized via MW-assisted hydrothermal method with a custom designed sugar ligand as the reducing agent. These NPs were conjugated with BQDs using EDC-NHS treatment. The hybrid Rh-GQDs NPs exhibited green emission (520 nm) under 490 nm excitation and led to a reduced cytotoxicity with respect to bare Rh NPs, highlighting the passivation role of the GQDs via the real-time cell analysis (RTCA) assay. The hybrid complex constituted a multimodal bioimaging contrastagent, tested with confocal microscopy (in vitro) and XRF phantom experiments.
Sedan deras upptäckt har nolldimensionella kvantprickar av grafen (kol) uppmärksammats inom biorelaterade applikationer, särskilt för deras optiska egenskaper, kemiska stabilitet och enkelt modifierbara yta. Denna avhandling fokuserar på en grön syntesmetod av kvävedopade grafen-kvantprickar för bimodal bioavbildning med röntgenfluorescens och optisk fluorescens. Både konventionella och mikrovågs-assisterade solvotermiska syntesmetoder användes för att undersöka metodernas effekt på de syntetiserade kvantprickarna. Den mikrovågs-assisterade metoden möjliggjorde syntes av uniforma kvantprickar med exciteringsoberoende egenskaper på grund av mycket kontrollerbara reaktionsförhållanden. Det demonstrerades att den molekylära strukturen hos prekursorerna påverkade de optiska fluorescensegenskaperna hos grafen-kvantprickarna. Genom att välja specifika prekursorer erhölls kvantprickar som emitterar i både blått och rött ljus, motsvarande emissionsmaxima vid 438 respektive 605 nm under excitering vid 390 respektive 585 nm. Amin-funktionaliserade Rh-nanopartiklar valdes som en aktiv kärna för röntgenfluorescens, syntetiserad genom en mikrovågs-assisterad hydrotermisk metod med en specialdesignad sockerligand som reduktionsmedel. Dessa nanopartiklar konjugerades med blåemitterande kvantprickar genom EDC-NHS-behandling. De hybrida nanopartiklarna uppvisade grön emission (520 nm) under 490 nm excitation och ledde till en minskad cytotoxicitet uppmätt genom cellanalys i realtid (RTCA) jämfört med endast Rh-nanopartiklar, vilket framhävde passiveringsrollen som kvantprickarna spelar. Hybridkomplexet utgjorde ett multimodalt kontrastmedel för bioavbildning, vilket demonstrerades med konfokalmikroskopi (in vitro) och fantomexperiment med röntgenfluorescens.
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Banerjee, Anusuya. "Novel, Targettable Bioimaging Probes Using Conjugates of Quantum Dots and DNA." Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066376/document.

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Les boîtes quantiques (ou Quantum Dots en anglais - QD) sont une nouvelle génération de sondes polyvalentes pour la biologie, en particulier pour l’imagerie. Pour des applications de marquage des voies intra-cellulaires, les QDs peuvent être conjugués à des biomolécules telles que des acides nucléiques ou des protéines. En partant des travaux du LPEM portant sur le développement de ligands permettant la dispersion des QDs dans l’eau et leur fonctionnalisation, une nouvelle méthode de conjugaison de l'ADN sur les QDs a été développée dans cette thèse. Cette méthode utilise les motifs présents sur les polymères des QDs pour le greffage d'ADN. Les paramètres affectant cette réaction ont été étudiés et cette stratégie de couplage a été étendue à d'autres nanoparticules et biomolécules. En partant de ces QDs-ADN, des protéines modifiées ADN ont pu être attachées aux QDs en utilisant le principe d’hybridation de l’ADN. Les propriétés des conjugués ainsi générés ont été mises en évidence en utilisant la Transferrine (QD-ADN-Tf) et ces complexes ont été étudiés in vitro et in cellulo. Ces conjugués ont ensuite été utilisés pour le suivi de la dynamique des endosomes, exploitant ainsi pleinement le potentiel des QDs pour l’imagerie directe. Dans la dernière partie, des études supplémentaires sur les facteurs influençant la «performance biologique» des QDs ont été réalisées. Pour cela, une large gamme de ligands polymères développée par le groupe a été utilisée pour sonder l'interaction de la surface des QDs avec l'interface biologique. Des expériences biochimiques et cellulaires ont permis de démontrer que les QDs revêtus de divers polymères ont des comportements différents
Quantum dots (QD) are new generation of versatile probes for biology, particularly for bioimaging. For specific applications, QDs are conjugated to biomolecules such as nucleic acid or proteins and subsequently targeted to unique intra-cellular pathways. Building upon the state-of-the-art ligands for water-dispersible QDs developed by the lab, a novel and highly generalizable method to conjugate DNA to QD is developed in this thesis. This method employs thiols present on polymers on QDs for conjugation to maleimide-functionalized DNA. Extensive characterization of parameters affecting this reaction is carried out and the strategy is extended to other nanoparticles and biomolecules. Following this, a novel method to conjugate proteins to QD via DNA hybridization is discussed. Using a model protein Transferrin (Tf), the unique properties of thus generated QD-DNA-Tf conjugates are studied in-vitro and in-cellulo. These conjugates are subsequently used for tracking endosomal dynamics for up-to 20 minutes, exploiting the fullest potential of QDs for live imaging. In the last part, additional studies on factors affecting the ‘biological performance’ of QDs are carried out. Using a range of highly adaptable polymeric ligands developed by the group, interactions of surface-modified QDs with the biological interface are probed. Systematic biochemical and cellular experiments demonstrate that QDs coated with zwitterionic polymers have superior antifouling properties compared to poly(ethylene glycol)-based polymers and stability in diverse biological contexts
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Books on the topic "Fluorescent Bioimaging"

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R, Viviani V., and Ohmiya Y, eds. Luciferases and fluorescent proteins: Principles and advances in biotechnology and bioimaging, 2007. Trivandrum: Transworld Research Network, 2007.

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Book chapters on the topic "Fluorescent Bioimaging"

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Xu, Jie, and Li Shang. "Fluorescent Metal Nanoclusters for Bioimaging." In Fluorescent Materials for Cell Imaging, 97–128. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-5062-1_5.

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Kailasa, Suresh Kumar, Vaibhavkumar N. Mehta, Nazim Hasan, and Hui-Fen Wu. "Fluorescent Carbon Dots for Bioimaging." In Advanced Bioelectronic Materials, 215–28. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2015. http://dx.doi.org/10.1002/9781118998861.ch6.

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Rawat, Dipti, and Ragini Raj Singh. "Fluorescent Magnetic Quantum Dots in Bioimaging." In Magnetic Quantum Dots for Bioimaging, 133–52. New York: CRC Press, 2023. http://dx.doi.org/10.1201/9781003319870-6.

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Di Martino, Miriam, Francesco Marrafino, Rosita Diana, Pio Iannelli, and Simona Concilio. "Fluorescent Probes for Applications in Bioimaging." In Advances in Bionanomaterials II, 243–58. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-47705-9_21.

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Li, Youbin, Songjun Zeng, and Jianhua Hao. "Lanthanide-Based Upconversion Nanoparticles for Bioimaging Applications." In Fluorescent Materials for Cell Imaging, 129–53. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-5062-1_6.

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Mohan, Nitin, and Huan-Cheng Chang. "Fluorescent Nanodiamonds and Their Prospects in Bioimaging." In Optical Engineering of Diamond, 445–71. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2013. http://dx.doi.org/10.1002/9783527648603.ch13.

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Sukumar, Uday Kumar, Arutselvan Natarajan, Tarik F. Massoud, and Ramasamy Paulmurugan. "Applications of Fluorescent Protein-Based Sensors in Bioimaging." In Topics in Medicinal Chemistry, 149–83. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/7355_2019_90.

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Xu, Hui, Lihua Wang, and Chunhai Fan. "Bioanalysis and Bioimaging with Fluorescent Conjugated Polymers and Conjugated Polymer Nanoparticles." In ACS Symposium Series, 81–117. Washington, DC: American Chemical Society, 2012. http://dx.doi.org/10.1021/bk-2012-1112.ch004.

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Wang, Dan, and Jun Qian. "AIE Luminogens for Three-Photon Fluorescence Bioimaging." In Principles and Applications of Aggregation-Induced Emission, 425–55. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-99037-8_18.

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Wang, Lei, and Guo-Bin Qi. "Supramolecular Self-assembled Nanomaterials for Fluorescence Bioimaging." In In Vivo Self-Assembly Nanotechnology for Biomedical Applications, 1–29. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-6913-0_1.

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Conference papers on the topic "Fluorescent Bioimaging"

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Reineck, Philipp, Amanda Abraham, Nicholas Nunn, Marco Torelli, Alexander Zaitsev, Adam Dalis, Neeraj Prabhakar, Olga Shenderova, and Brant C. Gibson. "Multicolor fluorescent nanodiamonds for bioimaging." In Biophotonics Australasia 2019, edited by Ewa M. Goldys and Brant C. Gibson. SPIE, 2019. http://dx.doi.org/10.1117/12.2539890.

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Zhang, Yong, and WenKai Li. "Upconverting Fluorescent Nanoparticles for Bioimaging and Therapy." In Asia Communications and Photonics Conference. Washington, D.C.: OSA, 2012. http://dx.doi.org/10.1364/acp.2012.as3e.1.

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Zhang, Yong, and WenKai Li. "Upconverting Fluorescent Nanoparticles for Bioimaging and Therapy." In Asia Communications and Photonics Conference. Washington, D.C.: OSA, 2012. http://dx.doi.org/10.1364/acpc.2012.as3e.1.

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Chatterjee, Dev K., and Yong Zhang. "Use of upconverting fluorescent nanoparticles for bioimaging." In SPIE OPTO, edited by Zameer U. Hasan, Philip R. Hemmer, Hwang Lee, and Charles M. Santori. SPIE, 2012. http://dx.doi.org/10.1117/12.905939.

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Baglietto, Silvia, Ibolya E. Kepiro, Gerrit Hilgen, Evelyne Sernagor, Vittorio Murino, and Diego Sona. "Segmentation of Retinal Ganglion Cells From Fluorescent Microscopy Imaging." In 4th International Conference on Bioimaging. SCITEPRESS - Science and Technology Publications, 2017. http://dx.doi.org/10.5220/0006110300170023.

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Ameli, Corrado, Sonja Fixemer, David Bouvier, and Alexander Skupin. "PRAQA: Protein Relative Abundance Quantification Algorithm for 3D Fluorescent Images." In 8th International Conference on Bioimaging. SCITEPRESS - Science and Technology Publications, 2021. http://dx.doi.org/10.5220/0010187400002865.

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Ameli, Corrado, Sonja Fixemer, David Bouvier, and Alexander Skupin. "PRAQA: Protein Relative Abundance Quantification Algorithm for 3D Fluorescent Images." In 8th International Conference on Bioimaging. SCITEPRESS - Science and Technology Publications, 2021. http://dx.doi.org/10.5220/0010187400210030.

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Nandy, Papiya, Debbethi Bera, Kunal Pal, Parimal Karmakar, and Sukhen Das. "Highly Fluorescent Carbon Nanoparticle: An Emerging Bioimaging Intervention." In MOL2NET 2020, International Conference on Multidisciplinary Sciences, 6th edition. Basel, Switzerland: MDPI, 2020. http://dx.doi.org/10.3390/mol2net-06-06786.

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Sharko, Olga, Maksim Tatulchenkov, Maksim Kvach, Sergey Pletnev, and Vadim Shmanai. "Nanoparticles-based fluorescent conjugates for MRI contrast agents and bioimaging." In 2015 5th International Workshop on Magnetic Particle Imaging (IWMPI). IEEE, 2015. http://dx.doi.org/10.1109/iwmpi.2015.7107068.

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Sokolov, I. L., A. V. Vasilyeva, A. V. Lunin, A. V. Yaremenko, and V. R. Cherkasov. "Fluorescent Superparamagnetic and Paramagnetic Agents for Bioimaging, Sensing and Cell Targeting." In 2018 International Conference Laser Optics (ICLO). IEEE, 2018. http://dx.doi.org/10.1109/lo.2018.8435453.

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