Relevant bibliographies by topics / Fluorescent analysis

Academic literature on the topic 'Fluorescent analysis'

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Published: 30 May 2022
Last updated: 31 May 2022

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Journal articles on the topic "Fluorescent analysis"

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Nurgaziyeva, Elmira, Sarkyt Kudaibergenov, Grigoriy Mun, and Vitaliy Khutoryanskiy. "Synthesis of fluorescently-labelled poly(2-ethyl-2-oxazoline)-protected gold nanoparticles." Chemical Bulletin of Kazakh National University, no. 1 (March 19, 2021): 12–20. http://dx.doi.org/10.15328/cb1185.

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Gold nanoparticles (GNPs) protected by poly(2-ethyl-2-oxazoline) (POZ) of different molecular weights (Mw = 5, 50, 200 and 500 kDa) were synthesised and characterised by dynamic light scattering, nanoparticle tracking analysis, zeta potential measurement and transmission electron microscopy. It was established that the use of POZ with 50 kDa resulted in formation of GNPs with low polydispersity while POZ with greater molecular weights led to formation of more polydisperse GNPs. Fluorescent labelling of these nanoparticles was achieved through their reaction with polyethyleneglycol dithiol (8-12 kDa) as a linker molecule with subsequent reaction with 6-(iodoacetamido) fluorescein. The fluorescent nature of obtained GNPs was confirmed by the appearance of the fluorescence peak at 510 nm that is typical for fluorescein molecules and glowing of the aqueous solution under the UV irradiaton. The fluorescently-labelled GNPs are promising tool in biomedical application to monitor the biological systems using fluorescent microscopy.
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Kim, In Jung, Yongbin Xu, and Ki Hyun Nam. "Spectroscopic and Structural Analysis of Cu2+-Induced Fluorescence Quenching of ZsYellow." Biosensors 10, no. 3 (March 23, 2020): 29. http://dx.doi.org/10.3390/bios10030029.

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Fluorescent proteins exhibit fluorescence quenching by specific transition metals, suggesting their potential as fluorescent protein-based metal biosensors. Each fluorescent protein exhibits unique spectroscopic properties and mechanisms for fluorescence quenching by metals. Therefore, the metal-induced fluorescence quenching analysis of various new fluorescent proteins would be important step towards the development of such fluorescent protein-based metal biosensors. Here, we first report the spectroscopic and structural analysis of the yellow fluorescent protein ZsYellow, following its metal-induced quenching. Spectroscopic analysis showed that ZsYellow exhibited a high degree of fluorescence quenching by Cu2+. During Cu2+-induced ZsYellow quenching, fluorescence emission was recovered by adding EDTA. The crystal structure of ZsYellow soaked in Cu2+ solution was determined at a 2.6 Å resolution. The electron density map did not indicate the presence of Cu2+ around the chromophore or the β-barrel surface, which resulted in fluorescence quenching without Cu2+ binding to specific site in ZsYellow. Based on these results, we propose the fluorescence quenching to occur in a distance-dependent manner between the metal and the fluorescent protein, when these components get to a closer vicinity at higher metal concentrations. Our results provide useful insights for future development of fluorescent protein-based metal biosensors.
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Shao, Lei, Longyu Zhang, Shilin Li, and Pengyuan Zhang. "Design and Quantitative Analysis of Cancer Detection System Based on Fluorescence Immune Analysis." Journal of Healthcare Engineering 2019 (December 24, 2019): 1–9. http://dx.doi.org/10.1155/2019/1672940.

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Human blood is an important medical detection index. With the development in clinical medical detection instruments and detection technology, the requirements for detection accuracy and efficiency have been gradually improved. Fluorescent immunochromatography is a new detection technique. It has the characteristics of high efficiency, convenience, no pollution, and wide detection range. Human blood can be detected quickly using fluorescent immunochromatography. At present, it has received great attention from the field of clinical testing. In this paper, a set of fluorescent immunochromatographic analyzer has been designed. It is mainly based on the principle of fluorescence immunochromatography. A new method of signal analysis and system design for fluorescent immunochromatography analyzer is proposed. By using the improved threshold function denoising algorithm, the quantitative detection of fluorescent immunochromatographic strip is realized. The concentration of pathogenic factors (cancer cells) in human serum can be measured conveniently and accurately. The system integrates many peripheral modules, including fluorescence signal acquisition, fluorescence signal processing, quantitative curve fitting, and test results. In this paper, the quantitative detection experiments of the system are carried out in three aspects: linearity, repeatability, and sensitivity. The experimental results show that the linear correlation coefficient is up to 0.9976, and the limit of detection is up to 0.05 ng/ml. The requirements of the system are satisfied. The system performance is good, and the quantitative result is accurate. Therefore, the establishment of a fluorescence analysis system is of great significance.
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Chugunova, М. M. "Metrology of fluorescent analysis in Russia and abroad." Metrologiya, no. 4 (2020): 52–68. http://dx.doi.org/10.32446/0132-4713.2020-4-52-68.

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Article contains overview of Russian and foreign reference materials of fluorescent properties, and possibility of their metrology using. Principle of sensitivity analysis for fluorescent instruments based on scaled relative fluorescence units are discussed. Technical and metrological characteristics of developed and certified in The All-Russian Research Institute for Optical and Physical Measurements fluorescence gauges kit based on organic dyes with various concentration are given.
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Liu, Mingxian, Fenglin Tang, Zhengli Yang, Jing Xu, and Xiupei Yang. "Recent Progress on Gold-Nanocluster-Based Fluorescent Probe for Environmental Analysis and Biological Sensing." Journal of Analytical Methods in Chemistry 2019 (January 2, 2019): 1–10. http://dx.doi.org/10.1155/2019/1095148.

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Gold nanoclusters (AuNCs) are one of metal nanoclusters, which play a pivotal role in the recent advances in the research of fluorescent probes for their fluorescence effect. They are favored by most researchers due to their strong stability in fluorescence and adjustability in fluorescence wavelength when compared to traditional organic fluorescent dyes. In this review, we introduce various synthesis strategies of gold-nanocluster-based fluorescent probes and summarize their application for environmental analysis and biological sensing. The use of gold-nanocluster-based fluorescent probes for the analysis of heavy metals and inorganic and organic pollutants is covered in the environmental analysis while biological labeling, imaging, and detection are presented in biological sensing.
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Waterman-Storer, C. M., and W. C. Salmon. "Fluorescent Speckle Microscopy in Studies of Cytoskeletal Dynamics During Cell Motility." Microscopy and Microanalysis 7, S2 (August 2001): 6–7. http://dx.doi.org/10.1017/s1431927600026106.

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We have discovered a new method, Fluorescent Speckle Microscopy (FSM), for analyzing the dynamic movement and turnover of macromolecular protein assemblies, such as the cytoskeleton, in living cells (Waterman-Storer et al., 1998). FSM compliments or replaces the techniques of fluorescence recovery after photobleaching or photoactivation of fluorescence for measuring protein dynamics in vivo. For FSM, cells are microinjected with a very low fraction of fluorescently labeled subunits that co-assemble with unlabeled subunits to give a structure with a fluorescent speckled appearance in diffraction-limited wide-field or confocal digital fluorescence images. At low fractions of fluorescent subunits relative to unlabeled subunits, fluorescent speckles vary randomly in intensity according to the number of fluorescent subunits within a diffraction-limited region. in time-lapse FSM image series, movement of the fluorescent speckle pattern indicates translocation of structures, while changes in speckle intensity indicate subunit turnover. We have used FSM to study microtubule and actin behavior in interphase and mitotic cells. We use kymograph analysis to quantitate the movement of speckled structures (Fig 1) and are currently developing analysis procedures to quantify subunit turnover in structures.We have applied these methods to the study of microtubule and actin cytoskeletal dynamics in migrating vertebrate cells in culture. Interactions between the microtubule and actin cytoskeletons underlie fundamental cellular processes such as cytokinesis and cell locomotion, but are poorly understood.
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Prapatpong, Pornpan, Thanayu Techa-In, Wantaporn Padungpuak, Sawanya Buranaphalin, and Leena Suntornsuk. "HPLC-Fluorescent Analysis of Memantine: An Investigation on Fluorescent Derivative Formation." Journal of Chemistry 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/672183.

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Memantine is an N-methyl-D-aspartate receptor antagonist recommended for treatment of Alzheimer’s disease. Due to the lack of chromophores/fluorophores in memantine molecule, this work focuses on a novel procedure for memantine-fluorescent derivative formation enabling it to be monitored by a fluorescence detector. 4-(N-Chloroformylmethyl-N-methyl)amino-7-N,N-dimethylaminosulphonyl 2,1,3-benzoxadiazole (DBD-COCl) was chosen as a fluorescent probe since the carbonyl chloride of DBD-COCl could easily react with the amine on memantine to form the fluorescent derivative. The derivatization was achieved at 5 : 1 of DBD-COCl and memantine, at 60°C for 50 min. Structure elucidation from mass spectrometry and infrared spectroscopy spectra confirmed the amide bond of memantine-DBD-COCl. The derivative was stable up to 24 h and could be monitored by high performance liquid chromatography (HPLC) coupled with a fluorescence detector using a VertiSep GES C18 column, acetonitrile and water (80 : 20) as the mobile phase with a flow rate of 1 mL/min using the excitation and emission wavelengths at 430 and 520 nm, respectively. The derivative was eluted at 4.50 min and was separated from the DBD-COCl. Preliminary validation data reveals good linearity (r2≥0.99), repeatability (%RSD < 0.54) and accuracy (%Rbetween 94 and 119%) with a limit of detection of 0.79 μg/mL.
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Zhou, Kun, Jian Tian, Qiushi Zhang, Xiangxi Meng, Kun Yang, and Qiushi Ren. "Simulation and quantitative analysis of fluorescence intensity distribution based on the Monte Carlo method." Journal of Innovative Optical Health Sciences 08, no. 06 (October 27, 2015): 1550038. http://dx.doi.org/10.1142/s1793545815500388.

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The Monte Carlo method is a versatile simulation algorithm to model the propagation of photons inside the biological tissues. It has been applied to the reconstruction of the fluorescence molecular tomography (FMT). However, such method suffers from low computational efficiency, and the time consumption is not desirable. One way to solve this problem is to introduce a priori knowledge which will facilitate iterative convergence. We presented an in vivo simulation environment for fluorescence molecular tomography (ISEFMT) using the Monte Carlo method to simulate the photon distribution of fluorescent objects and their sectional view in any direction quantitatively. A series of simulation experiments were carried out on different phantoms each with two fluorescent volumes to investigate the relationship among fluorescence intensity distribution and the excitation photon number, the locations and sizes of the fluorescence volumes, and the anisotropy coefficient. A significant principle was discovered, that along the direction of the excitation light, the fluorescent volume near the excitation point will provide shelter effect so that the energy of the fluorescent volume farther away from the excitation point is relatively lower. Through quantitative analysis, it was discovered that both the photon energy distribution on every cross section and the fluorescence intensity distributed in the two volumes exhibit exponential relationships. The two maximum positions in this distribution correspond to the centers of fluorescent volumes. Finally, we also explored the effect of the phantom coefficients on the exponential rule, and found out that the exponential rule still exists, only the coefficient of the exponential rule changed. Such results can be utilized in locating the positions of multiple fluorescent volumes in complicated FMT reconstruction involving multiple fluorescent volumes. Thus, a priori knowledge can be generalized, which would accelerate the reconstruction of FMT and even other images.
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Zhang, Zhihong, Heping Zhu, and Huseyin Guler. "Quantitative Analysis and Correction of Temperature Effects on Fluorescent Tracer Concentration Measurement." Sustainability 12, no. 11 (June 2, 2020): 4501. http://dx.doi.org/10.3390/su12114501.

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To ensure an accurate evaluation of pesticide spray application efficiency and pesticide mixture uniformity, reliable and accurate measurements of fluorescence concentrations in spray solutions are critical. The objectives of this research were to examine the effects of solution temperature on measured concentrations of fluorescent tracers as the simulated pesticides and to develop models to correct the deviation of measurements caused by temperature variations. Fluorescent tracers (Brilliant Sulfaflavine (BSF), Eosin, Fluorescein sodium salt) were selected for tests with the solution temperatures ranging from 10.0 °C to 45.0 °C. The results showed that the measured concentrations of BSF decreased as the solution temperature increased, and the decrement rate was high at the beginning and then slowed down and tended to become constant. In contrast, the concentrations of Eosin decreased slowly at the beginning and then noticeably increased as temperatures increased. On the other hand, the concentrations of Fluorescein sodium salt had little variations with its solution temperature. To ensure the measurement accuracy, correction models were developed using the response surface methodology to numerically correct the measured concentration errors due to variations with the solution temperature. Corrected concentrations using the models agreed well with the actual concentrations, and the overall relative errors were reduced from 42.36% to 2.91% for BSF, 11.72% to 1.55% for Eosin, and 2.68% to 1.17% for Fluorescein sodium salt. Thus, this approach can be used to improve pesticide sprayer performances by accurately quantifying droplet deposits on target crops and off-target areas.
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Stojanov, Spase, Tina Vida Plavec, Julijana Kristl, Špela Zupančič, and Aleš Berlec. "Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers." International Journal of Molecular Sciences 22, no. 24 (December 20, 2021): 13631. http://dx.doi.org/10.3390/ijms222413631.

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Lactobacilli are a promising natural tool against vaginal dysbiosis and infections. However, new local delivery systems and additional knowledge about their distribution and mechanism of action would contribute to the development of effective medicine. This will be facilitated by the introduction of the techniques for effective, inexpensive, and real-time tracking of these probiotics following their release. Here, we engineered three model vaginal lactobacilli (Lactobacillus crispatus ATCC 33820, Lactobacillus gasseri ATCC 33323, and Lactobacillus jensenii ATCC 25258) and a control Lactobacillus plantarum ATCC 8014 to express fluorescent proteins with different spectral properties, including infrared fluorescent protein (IRFP), green fluorescent protein (GFP), red fluorescent protein (mCherry), and blue fluorescent protein (mTagBFP2). The expression of these fluorescent proteins differed between the Lactobacillus species and enabled quantification and discrimination between lactobacilli, with the longer wavelength fluorescent proteins showing superior resolving power. Each Lactobacillus strain was labeled with an individual fluorescent protein and incorporated into poly (ethylene oxide) nanofibers using electrospinning, as confirmed by fluorescence and scanning electron microscopy. The lactobacilli retained their fluorescence in nanofibers, as well as after nanofiber dissolution. To summarize, vaginal lactobacilli were incorporated into electrospun nanofibers to provide a potential solid vaginal delivery system, and the fluorescent proteins were introduced to distinguish between them and allow their tracking in the future probiotic-delivery studies.
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Dissertations / Theses on the topic "Fluorescent analysis"

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Mangham, Barry. "Synthesis and analysis of fluorescent dye molecules." Thesis, University of Nottingham, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.602528.

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The nature of non-covalent bonding interactions was investigated through the deposition and subsequent scanning tunnelling microscopy (STM) imaging of tetra-substituted porphyrins. Porphyrins bearing iodo, bromo, nitro, pyridyl and carboxylic acid groups were synthesised and deposited on either Au(110) or Au(l11). STM imaging and analysis showed a variety of different orientations and packing for the different functional groups. The unusual tip induced growth of honeycomb packing orientations was seen for tetra-pyridyl substituted porphyrin. Tetra-bromo substituted porphyrin was observed to adopt different ordered orientations of the saddle shape conformation on Au(111). The synthesis of novel porphyrin dimers bearing carboxylic acid groups was investigated, with a variety of different pathways being identified and explored. Furthermore, upon cooling unusual spectroscopic behaviour was observed for a hexa-phenyl substituted meso-linked porphyrin dim er. The synthesis of novel BODIPY dimers and trimers was investigated. A number of fluoro and catecholate substituted BODIPY compounds were synthesised, bearing a variety of different linkers. Linkers investigated included phenyl, biphenyl, terphenyl, durene and terphenylene. Electrochemical and spectroscopic investigations demonstrated a variety of differences between meta- and para-substitution positions. The extension of the linker length from phenyl through to terphenyl displayed a reduction in communication of BODIPY moieties. The durene linked dimer added steric bulk to the centre of the BODIPY dimer, resulting in increased fluorescence lifetimes and quantum yields. 1
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Ladyman, Melissa Kate. "Development of fluorescent assays for biological analysis." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/17627.

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The work in this thesis is divided into two parts; the first is the synthesis of a ‘switch-on’ fluorophore to measure cell viability, and the second is the development of a fluorescent detection method for protein−peptide affinity assays applied in the identification of protein-protein inhibitors. Tetrazolium salts are often used in cytotoxicity assays as indicators of cell viability as they are reduced to deeply coloured formazans exclusively in healthy cells. However, measuring the absorbance of the formazan is prone to bias from other coloured species in the cell media, requires solubilisation and can be difficult to quantify. A preferable method of detection is direct fluorescence as it is easily quantified, more sensitive and would ideally remove the need to solubilise the insoluble dye. The aim of this project was to synthesise a tetrazolium salt that could be reduced to a soluble fluorescent formazan in healthy cells as an indicator of cell viability. A number of fluorescent formazans were synthesised by incorporation of a fluorophore. The corresponding tetrazolium salts were non-fluorescent and could be reduced to the formazan in vitro. Several formazans were synthesised to attempt to increase the emission wavelength and intensity to overcome cellular autofluorescence. Protein-protein interactions have been implicated in the pathogenesis of many human diseases but until recently were considered undruggable. However, peptides have emerged as ideal compounds for targeting the large and relatively featureless protein interfaces. Work focussed on the discovery of peptide inhibitors for the E3 ubiquitin ligase stationary-phase kinase associated protein (Skp2). Potential peptide inhibitors were identified using CelluSpot synthesis and array technology to screen peptide libraries. Qualitative analysis of the protein affinity assay results by enhanced chemiluminescent detection was found to be misleading, and so a quantifiable and more sensitive fluorescent detection method was developed.
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Martin, Andrew. "Glycosylated green fluorescent protein for carbohydrate binding protein analysis." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/glycosylated-green-fluorescent-protein-for-carbohydrate-binding-protein-analysis(9ddae46e-b4d7-4c08-8240-94b9b804ac68).html.

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The interactions of glycoconjugates with carbohydrate binding proteins are responsible for a wide range of recognition events in vivo; including immune response, cell adhesion and signal transduction. Glycoconjugates have already found many medicinal uses as therapeutic and diagnostic agents, but their full potential is yet to be realised. Access to a variety of homogeneously glycosylated glycoproteins is essential for the study of these important carbohydrate binding events. This requires the chemical synthesis and attachment of biologically relevant glycans to unglycosylated protein scaffolds in a site selective manner. Here we describe the use of a range of glycosyl iodoacetamides to glycosylate proteins selectively via their cysteine residues. We have chosen the green fluorescent protein mutant GFPuv for use as a protein scaffold due its known tolerance of two cysteine mutations (E6C and I229C) and the previous successful derivatisation of these cysteines with iodoacetamides.1 The inherent fluorescence of GFPuv also makes it a useful candidate for fluorescence based binding assays or cell labelling studies.16 active, mutants of GFPuv were created using a mixture of site directed mutagenesis and DNA shuffling (including one mutant containing six reactive cysteine residues). This was achieved by producing random combinations of two synthetic variants of GFPuv, one of which contained 33 surface cysteines. 94 bacterial colonies expressing active GFPuv were then sequenced and the new chimeric genes analysed. Four monosaccharides and one trisaccharide (N-glycan core mimic) suitable for the chemical glycosylation via cysteines were synthesised and successfully used to create a selection of homogeneous neoglycoproteins. These neoglycoproteins were demonstrated to interact differently with different lectins (including ConA, GNL and Jacalin) in a qualitative fluorescence based assay. Interactions were shown to vary with glycan structure, position of glycosylation sites and the number of glycosylation sites.
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Mohammadi, Mahnaz. "Developing an imaging bi-spectrometer for fluorescent materials /." Online version of thesis, 2009. http://hdl.handle.net/1850/9671.

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Hunter, S. Jayne. "The quantitative analysis of green fluorescent protein in plants." Thesis, University of Sheffield, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340248.

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Manolakis, Alexis. "A comparative analysis of traditional and fluorescent spermatozoa staining techniques." Thesis, Boston University, 2012. https://hdl.handle.net/2144/12502.

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Thesis (M.S.)--Boston University PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.
The purpose of this study was to compare the traditional KPIC method for staining sperm to modern fluorescent techniques employed by SPERM HY-LITERTM. Factors such as staining time, sperm recovery, and the ability to quickly and accurately count sperm were evaluated. Dilutions ranging from 1:2 to 1:10,000,000 of semen, post-coital swabs and sperm/epithelial cell suspensions were stained with SPERM HY-LITERTM, SPERM HY-LITERTM Express and KPIC. Case work samples were also analyzed with KPIC and SPERM HY-LITERTM. The sperm counts for the more concentrated dilutions of semen and sperm/e-cell samples were statistically similar in both KPIC and SPERM HY-LITERTM. However, post-coital samples stained with SPERM HY-LITERTM were statistically different than samples stained with KPIC, resulting in the observation of fewer cells. Similar results were observed with SPERM HY-LITERTM Express as well as with case work samples stained with SPERM HY-LITERTM. Although SPERM HY-LITERTM has the benefits of quicker and easier scanning due to the fluorescent filters, it is considerably more costly to employ and did not result in greater sperm recovery. Overall, KPIC provided the best results in the shortest amount of time and within a reasonable budget.
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Wu, Yongqi. "Multi-parameter Fluorescent Analysis of Magnetically Enriched Circulating Tumor Cells." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1408633549.

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Cain, Heather. "Fluorescent derivatization of arginine- and carbonyl-containing analytes for analysis by capillary electrophoresis with laser-induced fluorescence detection." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0007/MQ59786.pdf.

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Hu, Qin. "Synthesis, characterization and analytical separation of fluorescent water-soluble carbon nanoparticles." HKBU Institutional Repository, 2015. https://repository.hkbu.edu.hk/etd_oa/156.

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This thesis mainly consists of two parts. The first part is concentrated on synthesizing amine/carboxylic-functionalized carbon nanoparticles (CNP) and investigating its fundamental properties in the aid of capillary zone electrophoresis (CZE) and high-performance liquid chromatography (HPLC). In this part, CNP is synthesized from citric acid (CA) and 1,2-ethylenediamine (EDA) by a microwave-assisted pyrolysis method. The resultant CNP is characterized by absorption and photoluminescence (PL) spectroscopy, transmission electron microscopy (TEM) and infrared spectroscopy (IR) to determine its overall optical properties, morphology and composition. Followed by this, the CNP product is separated and analyzed by CZE coupled with UV absorption and laser-induced fluorescence (LIF) detections. Under optimal pH and concentration of run buffer, the effect of reaction time and mole ratio of amine (NH2) to carboxylic acid (COOH) moieties of the precursors on the CNP species present in CNP products are studied. Our results show that the synthesis of CNP could be improved by lengthening the microwave irradiation time and optimizing the initial mole ratio of NH2/COOH in the precursors. Negatively charged CNP are obtained only when the amount of CA exceeds that of EDA, i.e., the mole ratio of NH2/COOH is 0.250.80. By contrast, when the quantity (in mole) of NH2 in EDA is equal to or larger than that of COOH in CA, only positively charged and neutral CNP species are formed, inferring that the CNP species are predominantly covered by the surface-attached ammonium and amido moieties. This work highlights the merit of CZE to identify the composition of an as-prepared CNP product which is pretty much dependent on the mole ratio of NH2/COOH. In addition, we carry out reversed-phase high-performance liquid chromatography coupled with fluorescence detection (RP-HPLC-FD) methodology to study the properties of each individual CNP species. Under optimal mobile phase and elution gradient conditions, the effect of mole ratio of NH2/COOH in the initial reagents on CNP product is studied. At NH2/COOH = 0.67, the strongest fluorescence CNP sample is obtained. The separated CNP fractions are collected and further characterized by UV-visible absorption and PL spectroscopy, CE, TEM, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The absorption and PL emission bands (λem) of the fractions are bathochromatically shifted with the elution order of CNP on RP-HPLC. The TEM images prove that CNP are eluted from the smallest to the largest. The MALDI-TOF MS data show that CNP undergo fragmentations, closely relating to their surface-attached carboxylic acid and amide/amine moieties. This work highlights the merit of RP-HPLC coupled with fluorescence detection, TEM and MS for isolation and characterization of individual CNP species present in a CNP sample. By application of CE and HPLC separation for CNP product, better understandings of the hidden fundamental properties of CNP are achieved. The two separation techniques are well complementary to each. On one hand, CE is able to separate both positively and negatively charged CNP species which cannot be retained and separated by HPLC column, facilitating the investigation of different charge states of CNP species present in a CNP product. On the other hand, the preparative property of HPLC allows for multi-collection of the separated CNP fractions which is difficult in the case of CE analysis owning to its low sample injection volume. By using HPLC separation, the individual CNP fractions can be collected for more precious investigation of their unique photophysical and chemical properties by absorption and PL spectroscopy, TEM and MALDI-TOF MS. The second part is focused on preparing CNP from naturally available bioresources and investigating the effect of doped heteroatoms on nitrogen (N) and sulfur (S)-doped carbon nanoparticles (N,S-CNP) based on our proposed modern RP-HPLC-FD methodology which has been proved to be useful in separating and analyzing CNP in the first part of this work. In this part, ultrasmall N,S-CNP is prepared by microwave-assisted pyrolysis of precursors of rice as carbon source and N-acetyl-L-cysteine (NAC) as N and S dopants. The obtained N,S-CNP are fully characterized by elemental analysis, FTIR, x-ray photoelectron spectroscopy, TEM, UV-vis absorption and PL spectroscopy. Meanwhile, undoped CNP (derived from rice only) is also synthesized and characterized. The chemical compositions, sizes and spectral properties of undoped CNP (derived from rice only) and N,S-CNP are demonstrated to be different from each other. With the assistance of RP-HPLC-FD, the effect of different mass ratios of NAC to rice (NAC/rice) on N,S-CNP is investigated. When the NAC/rice increases from 0.20 to 0.80, the signals of the later eluted peaks increase progressively, indicating that higher NAC/rice benefits the generation of CNP with stronger fluorescence emissions. The HPLC-separated N,S-CNP fractions are collected and further characterized by MALDI-TOF MS, UV-vis absorption and PL spectroscopy, showing that the structural changes induced by doping with heteroatoms N and S plays a key role in regulating the PL properties of the N,S-CNP
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Chen, Kai. "Use of green fluorescent protein for the analysis of protein-protein and protein-DNA interactions." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/4886.

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Restriction modification (RM) systems play a crucial role in preventing the entry of foreign DNA into the bacterial cell. The best studied Type I RM system is EcoKI from Escherichia coli K12. Both bacteriophage and conjugative plasmids have developed a variety of strategies to circumvent the host RM system. One such strategy involves the production of antirestriction proteins that mimic a short segment of DNA and efficiently inhibit the RM system. The main aim of this project was to analyse the interaction of EcoKI and its cognate methylase (MTase) with the T7 antirestriction protein, known as overcome classical restriction (Ocr), and various ArdA antirestriction proteins. Currently, there is a paucity of structural data on the complex formed between the Type I system and the antirestriction proteins. The aim of this work was twofold; (i) compare the interaction of MTase with DNA and Ocr and (ii) quantify the strength of interaction between MTase and various ArdA proteins. The MTase was fused to the Green Fluorescent Protein (GFP) to facilitate determination of the orientation of interaction with DNA and Ocr. Time resolved fluorescence measurements were carried out using the GFP-MTase fusion to determine the fluorescence lifetime and anisotropy decay. These experiments were conducted using a time resolved fluorescence instrument fabricated in-house. The values determined in these experiments were then used to perform fluorescence resonance energy transfer (FRET) measurements with fluorescently labelled DNA or Ocr. These measurements gave information concerning the relative orientation of the MTase with either DNA or Ocr. The GFP-MTase fusion was also used to quantify the strength of interaction with various ArdA proteins. Previous attempts to determine the strength of interaction between MTase and ArdA proteins by employing conventional techniques have been unsuccessful. Therefore, a novel method was developed that exploits the interaction of MTase with a cation exchange medium, which can subsequently be displaced upon binding to ArdA. This method facilitated the determination, for the first time, of a set of binding affinities for the MTase and ArdA interaction.
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Books on the topic "Fluorescent analysis"

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International Symposium in Honor of Gregorio Weber's Seventieth Birthday (1986 Bocca di Magra, Italy). Fluorescent biomolecules: Methodologies and applications. New York: Plenum Press, 1989.

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Flunder, Keith. An investigation of the fluorescence of British bituminous coals: The analysis of fluorescent spectra of coal macerals. Birmingham: Aston University. Department of Chemical Engineering and Applied Chemistry, 1989.

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Lamb, Jeremy Charles. Fluorescent derivatives of tubulin as probes for the analysis of microtubule dynamics. Norwich: University of East Anglia, 1985.

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Goldman, Alastair Simon Howard. The segregation of normal and translocated chromosomes in human male meiosis: Analysis utilizing fluorescent in situ hybridisation. Birmingham: University of Birmingham, 1993.

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Putnam, Larry D. Analysis of ground-water flow in the Madison aquifer using fluorescent dyes injected in Spring Creek and Rapid Creek near Rapid City, South Dakota, 2003-04. Reston, Va: U.S. Geological Survey, 2007.

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service), SpringerLink (Online, ed. Fluorescent Proteins II: Application of Fluorescent Protein Technology. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012.

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Energy dispersive x-ray fluorescence analysis. Warszawa: PWN, 1989.

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Klockenkämper, R. Total-reflection X-ray fluorescence analysis. New York: Wiley, 1997.

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Total reflection X-ray fluorescence analysis. New York: Wiley, 1997.

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Jenkins, Ron. X-Ray fluorescence spectroscopy. New York: Wiley, 1988.

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Book chapters on the topic "Fluorescent analysis"

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Bayley, Peter, and Stephen Martin. "Analysis of Complex Fluorescence Decay Data." In Fluorescent Biomolecules, 389–92. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4684-5619-6_32.

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Kubínová, Lucie, Marie Jirkovská, Petr Hach, Daniel Palouš, Petr Karen, and Ivan Krekule. "Application of Confocal Microscopy to 3-D Reconstruction and Morphometrical Analysis of Capillaries." In Fluorescence Microscopy and Fluorescent Probes, 285–89. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_44.

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Modesti, Mauro. "Fluorescent Labeling of Proteins." In Single Molecule Analysis, 101–20. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-282-3_6.

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Modesti, Mauro. "Fluorescent Labeling of Proteins." In Single Molecule Analysis, 115–34. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7271-5_6.

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Fung, Jia Jun, Karla Blöcher-Juárez, and Anton Khmelinskii. "High-Throughput Analysis of Protein Turnover with Tandem Fluorescent Protein Timers." In Methods in Molecular Biology, 85–100. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-1732-8_6.

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AbstractTandem fluorescent protein timers (tFTs) are versatile reporters of protein dynamics. A tFT consists of two fluorescent proteins with different maturation kinetics and provides a ratiometric readout of protein age, which can be exploited to follow intracellular trafficking, inheritance and turnover of tFT-tagged proteins. Here, we detail a protocol for high-throughput analysis of protein turnover with tFTs in yeast using fluorescence measurements of ordered colony arrays. We describe guidelines on optimization of experimental design with regard to the layout of colony arrays, growth conditions, and instrument choice. Combined with semi-automated genetic crossing using synthetic genetic array (SGA) methodology and high-throughput protein tagging with SWAp-Tag (SWAT) libraries, this approach can be used to compare protein turnover across the proteome and to identify regulators of protein turnover genome-wide.
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Heller, Michael J. "Fluorescent Detection Methods for PCR Analysis." In The Polymerase Chain Reaction, 134–41. Boston, MA: Birkhäuser Boston, 1994. http://dx.doi.org/10.1007/978-1-4612-0257-8_11.

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Horan, Paul Karl, Sue E. Slezak, and Bruce D. Jensen. "Cellular Proliferation History by Fluorescent Analysis." In Flow Cytometry, 133–39. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8_8.

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Pan, Yu-Ching E., and Stanley Stein. "Amino Acid Analysis With Postcolumn Fluorescent Derivatization." In Methods of Protein Microcharacterization, 105–19. Totowa, NJ: Humana Press, 1986. http://dx.doi.org/10.1007/978-1-59259-436-8_4.

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Utaka, Tadashi, and Tomoya Arai. "Instrumentation for Total Reflection Fluorescent X-Ray Spectrometry." In Advances in X-Ray Analysis, 933–40. Boston, MA: Springer US, 1992. http://dx.doi.org/10.1007/978-1-4615-3460-0_27.

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Salnikow, Johann, Zbigniew Palacz, and Brigitte Wittmann-Liebold. "Automated Solid Phase-Sequencing Using Fluorescent Edman Reagents." In Methods in Protein Sequence Analysis · 1986, 247–60. Totowa, NJ: Humana Press, 1987. http://dx.doi.org/10.1007/978-1-59259-480-1_16.

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Conference papers on the topic "Fluorescent analysis"

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Kinahan, David J., Tara M. Dalton, and Mark R. Davies. "Microchannel Fluorescent Melting Curve Analysis." In ASME 2008 6th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2008. http://dx.doi.org/10.1115/icnmm2008-62014.

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In recent years, Fluorescent Melting Curve Analysis (FMCA) has become an almost ubiquitous feature of commercial quantitative PCR (qPCR) thermal cyclers. Here a microfluidic device is presented capable of performing droplet based Fluorescent Melting Curve Analysis within a microchannel. The device consists of modular thermally conductive blocks which can sandwich a microfluidic substrate. Opposing ends of the blocks are held at differing temperatures and a linear thermal gradient is generated along the microfluidic channel. Fluorescent measurements taken from a sample as it passes along the micro-fluidic channel permits fluorescent melting curves be generated. In this study we demonstrate the capability of measuring fluorescent melt curves from DNA passing through the device in a serial flow of sample plugs buffered by mineral oil.
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Sayers, Michael B., and Tara M. Dalton. "A Real-Time Continuous Flow Polymerase Chain Reactor for DNA Expression Quantification." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-43058.

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Real-time quantitative Polymerase Chain Reaction (PCR) is an extremely sensitive and reliable method for quantifying gene expression, allowing subtle shifts in gene expression to be easily monitored. Currently, stationary real-time PCR is readily achieved using fluorescent labels which increase in fluorescence as the DNA is exponentially amplified. Quantitative PCR is used in a myriad of applications. However currently most commercial real-time PCR devices are batch process stationary well based systems, limiting their throughput. Continuous flow microfluidic PCR devices have allowed for advancement in terms of improved PCR throughput and reduced reagent usage. As part of an overall total analysis system a device integrating all the functional steps of continuous flow realtime quantitative PCR has been designed and fabricated. Initially the PCR reaction mixture is segmented into nano-litre PCR reactors which are then thermally cycled on a two temperature fifty cycle flow-through PCR device, which allows laser induced fluorescent imaging of the nanoreactors. Previous studies into continuous flow PCR have demonstrated endpoint fluorescent measurements, however this research allows PCR nanoreactors to be fluorescently monitored after every PCR thermal cycle. Fluorescent optical monitoring is achieved through laser excitation of the nanoreactors while a Charged Coupled Device (CCD) camera is used to record the fluorescent emissions from the nanoreactors. Intensity analysis of the recorded images is then preformed using MATLAB to accurately determine the fluorescence intensity level, thereby allowing real-time quantitative amplification curves to be generated. This has major advantages over existing continuous flow PCR devices which use endpoint fluorescence and capillary electrophoresis, as the amplification curves allow far more information to be gleaned and allow the initial DNA template concentration to be accurately determined.
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Chen, Tongsheng, Shaoqun Zeng, Qingming Luo, Zhihong Zhang, and Wei Zhou. "Data analysis in green-fluorescent-protein-based fluorescence resonance energy transfer." In Optics and Optoelectronic Inspection and Control: Techniques, Applications, and Instruments, edited by Hong Liu and Qingming Luo. SPIE, 2000. http://dx.doi.org/10.1117/12.403990.

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Dalton, Tara M., David J. Kinahan, and Mark R. Davies. "Fluorescent Melting Curve Analysis Compatible With a Flowing Polymerase Chain Reactor." In ASME 2005 International Mechanical Engineering Congress and Exposition. ASMEDC, 2005. http://dx.doi.org/10.1115/imece2005-80954.

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A primary tool for analysing PCR product is the Fluorescent Melting Curve Analysis (FMCA). The temperature at which a double helix DNA strand denatures depends both on its length and base pair composition. Accurate measurement of this melting temperature using fluorescence allows estimations be made regarding DNA product length and composition. Current progress in development of PCR thermal cyclers has been primarily aimed at micro-channel based flowing devices. This paper addresses the challenges associated with performing FMCA analysis which is compatible with the output from a flowing PCR thermocycler. Two PCR products of significantly different lengths and base pair composition are compared using space domain FMCA. Results allow for differentiation of the PCR product, and compare favourably with results from a commercial thermal cycler. The successful application of FMCA within a channel shows its potential for use in high throughput flow based total analysis systems (μTAS).
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Barton, D. L., and P. Tangyunyong. "Scanning Fluorescent Microthermal Imaging." In ISTFA 1997. ASM International, 1997. http://dx.doi.org/10.31399/asm.cp.istfa1997p0041.

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Abstract We have developed scanning fluorescent microthermal imaging (SFMI), a new failure analysis technique. The fluorescent microthennal imaging (FMI) technique has been used for over a decade in its original form [1-2]. FMI normally relies on the use of a cooled, slow-scan CCD camera and a flood beam fluorescence pump source, usually an ultraviolet arc lamp. Interest in FMI has grown greatly over the past few years [3-9] due largely to its unique combination of high spatial and thermal resolution. In this paper, we demonstrate that the existing infrastructure found on a scanning laser microscope (SLM) is capable of acquiring the necessary images for SFMI using its scanned laser source and a point detector. The implications ofthis work are significant in that now high spatial and thermal resolution images can be made using an SLM without the need of additional, expensive hardware.
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Schertzer, Michael J., Peter Lea, Ridha Ben-Mrad, and Pierre E. Sullivan. "Effect of Surface Modification on Protein Deposition in Desiccated Droplets." In ASME 2014 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/imece2014-36789.

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Detection of fluorescent signals from desiccated droplets is a useful tool for the analysis of microarray devices. This investigation presents an optical method for the observation of physical structures and protein deposition in desiccated droplets. Greyscale images of droplets containing red fluorescent protein solutions in water were recorded after desiccation. Images were obtained under both white and fluorescent light for droplets desiccated on glass and Teflon. Spots desiccated on glass were twice the size of those desiccated on Teflon. As such, average physical deposition and protein concentration was higher for Teflon spots. The average fluorescence intensity for spots desiccated on Teflon were four times greater than those desiccated on glass. For spots desiccated on glass, physical deposition and protein concentration increased with radial position, consistent with a coffee-ring pattern. The local maximum fluorescence intensity was highest at the center of the droplet. Protein deposition then decreased with increasing radius before increasing again toward the edge of the spot. These results suggest that desiccation of protein laden droplets on hydrophobic coatings, such as Teflon, may increase sensitivity of fluorescent protein detection while improving the uniformity of the fluorescent signal measured from the droplet.
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Wulfman, David R., Tracey Baas, and Ronald McGlennen. "Planar Waveguide: Utility in Amino Acid Detection." In ASME 2003 International Electronic Packaging Technical Conference and Exhibition. ASMEDC, 2003. http://dx.doi.org/10.1115/ipack2003-35300.

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A device for the detection of fluorescently labeled nucleic acid sequences immobilized or hybridized to the surface of a planar waveguide has been developed. The device described requires no image gathering or analysis utilities, nor any specially patterned waveguide surface for detection. The system consists of an excitation light source and a photo detector, filters for select fluorescent emissions and a standard glass microscope slide that functions as a planar waveguide (PWG) and assay substrate. The device can discriminate fluorescent DNA products hybridized to a glass based array as effectively as laser based reader instruments, and is a low cost system with a high potential for full automation. The system’s functional parameters are presented along with design schematics of current prototypes. Performance data is also presented showing test results comparable to pre-established fluorescence detection means. Future design goals are also discussed. It is concluded that as a component in telemedicine or other remote medical care strategies, the detection means presented can play a significant role in bringing molecular diagnosis and gene detection to arenas of medical care where it is currently unavailable.
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Шадрина, Мария Михайловна, Николай Михайлович Немирович-Данченко, and Марина Юрьевна Ходанович. "METHOD OF IMMUNOHISTOCHEMICAL AND FLUORESCENT HYBRIDIZATION ANALYSIS." In Высокие технологии и инновации в науке: сборник избранных статей Международной научной конференции (Санкт-Петербург, Сентябрь 2020). Crossref, 2020. http://dx.doi.org/10.37539/vt187.2020.64.12.002.

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Нами разработан протокол совмещения иммуногистохимического окрашивания маркера молодых нейронов даблкортина и флуоресцентной гибридизации in situ (FISH) теломерной ДНК на криосрезах мозга толщиной 10 нм. Показано успешное применение, приводится подробный протокол окрашивания. We have developed a technique for joint staining of brain 10 nm criosections for doublecortin immunohistochemistry and telomere DNA fluorescent in situ hybridization. The successful application of the technique is shown, a timetable procedure is developed and all the reagents necessary for its implementation are written out, as well as the necessary temperature conditions are indicated.
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Coppeta, Jonathan, and Chris Rogers. "A quantitative mixing analysis using fluorescent dyes." In 34th Aerospace Sciences Meeting and Exhibit. Reston, Virigina: American Institute of Aeronautics and Astronautics, 1996. http://dx.doi.org/10.2514/6.1996-539.

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Menchen, Steve, Scott Benson, Krishna Upadhya, Pete Theisen, Joan Hauser, and Paul Kenney. "Fluorescent labeling of DNA for genetic analysis." In BiOS 2000 The International Symposium on Biomedical Optics, edited by Patrick A. Limbach, John C. Owicki, Ramesh Raghavachari, and Weihong Tan. SPIE, 2000. http://dx.doi.org/10.1117/12.380498.

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Reports on the topic "Fluorescent analysis"

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Guttinger, Kimberly. Multi-Dimensional Analysis of Fluorescent Chemosensor Data. Portland State University Library, January 2015. http://dx.doi.org/10.15760/honors.194.

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Deng, Chun, Zhenyu Zhang, Zhi Guo, Hengduo Qi, Yang Liu, Haimin Xiao, and Xiaojun Li. Assessment of intraoperative use of indocyanine green fluorescence imaging on the number of lymph node dissection during minimally invasive gastrectomy: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, November 2021. http://dx.doi.org/10.37766/inplasy2021.11.0062.

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Review question / Objective: Whether is indocyanine green fluorescence imaging-guided lymphadenectomy feasible to improve the number of lymph node dissections during radical gastrectomy in patients with gastric cancer undergoing curative resection? Condition being studied: Gastric cancer was the sixth most common malignant tumor and the fourth leading cause of cancer-related death in the world. Radical lymphadenectomy was a standard procedure in radical gastrectomy for gastric cancer. The retrieval of more lymph nodes was beneficial for improving the accuracy of tumor staging and the long-term survival of patients with gastric cancer. Indocyanine green(ICG) near-infrared fluorescent imaging has been found to provide surgeons with effective visualization of the lymphatic anatomy. As a new surgical navigation technique, ICG near-infrared fluorescent imaging was a hot spot and had already demonstrated promising results in the localization of lymph nodes during surgery in patients with breast cancer, non–small cell lung cancer, and gastric cancer. In addition, ICG had increasingly been reported in the localization of tumor, lymph node dissection, and the evaluation of anastomotic blood supply during radical gastrectomy for gastric cancer. However, it remained unclear whether ICG fluorescence imaging would assist surgeons in performing safe and sufficient lymphadenectomy.
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Liu, Ben. Effects of fluorescent light cystoscopy in non-muscle-invasive bladder: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review Protocols, April 2020. http://dx.doi.org/10.37766/inplasy2020.4.0178.

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Augustoni, Arnold L. Hazard analysis of long term viewing of visible laser light off of fluorescent diffuse reflective surfaces (post-it). Office of Scientific and Technical Information (OSTI), October 2006. http://dx.doi.org/10.2172/894322.

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Yenen, O., K. W. McLaughlin, and D. H. Jaecks. Alignment of Ar{sup +} [{sup 3}P]4p{sup 2}P{sup 0}{sub 3/2} satellite state from the polarization analysis of fluorescent radiation after photoionization. Office of Scientific and Technical Information (OSTI), April 1997. http://dx.doi.org/10.2172/603600.

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Dunn, Robert C., Heath A. Huckabay, Denise L. Lee, and Brian W. Ticknor. Analysis of Environmental Swipes Using Fluorescence Microscopy - Summary Report. Office of Scientific and Technical Information (OSTI), May 2017. http://dx.doi.org/10.2172/1424438.

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Gilfrich, John V., and W. T. Elam. X-Ray Fluorescence Analysis at the Naval Research Laboratory. Fort Belvoir, VA: Defense Technical Information Center, March 1998. http://dx.doi.org/10.21236/ada340458.

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Jurgensen, A., D. David Missimer, and R. Ronny Rutherford. X-RAY FLUORESCENCE ANALYSIS OF HANFORD LOW ACTIVITY WASTE SIMULANTS. Office of Scientific and Technical Information (OSTI), May 2006. http://dx.doi.org/10.2172/891691.

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Ning Gao. Radiochemical Analysis by High Sensitivity Micro X-Ray Fluorescence Detection. Office of Scientific and Technical Information (OSTI), May 2006. http://dx.doi.org/10.2172/882477.

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Morkoc, Hadis. RHEEDAX Induced X-ray Fluorescence Analysis System for Oxide MBE. Fort Belvoir, VA: Defense Technical Information Center, March 2008. http://dx.doi.org/10.21236/ada478811.

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