Dissertations / Theses on the topic 'Fluorescence Recovery while photobleaching'
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Gousseva, Veronika. "A quantitative fluorescence recovery after photobleaching analysis of receptor-protein interactions on vesicles /." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=101130.
Full textRodriguez-Enriquez, Ricardo. "Analysis of Bcl-2 family protein interactions in live cells by fluorescence recovery after photobleaching." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/analysis-of-bcl2-family-protein-interactions-in-live-cells-by-fluorescence-recovery-after-photobleaching(aa5eb271-6e43-48f3-940d-f63763ea4629).html.
Full textGaffield, Michael A. "FRAP measurements of synaptic vesicle mobility in motor nerve terminals /." Connect to abstract via ProQuest. Full text is not available online, 2007.
Find full textTypescript. Includes bibliographical references (leaves 84-93). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;
Travascio, Francesco. "Modeling Molecular Transport and Binding Interactions in Intervertebral Disc." Scholarly Repository, 2009. http://scholarlyrepository.miami.edu/oa_dissertations/322.
Full textInnhausen, u. Knyphausen Adrian zu [Verfasser], and Ralph [Akademischer Betreuer] Rupp. "A novel method for Fluorescence Recovery after Photobleaching (FRAP) analysis of chromatin proteins in pluripotent embryonic cells of the South African clawed frog X. laevis / Adrian zu Innhausen u. Knyphausen ; Betreuer: Ralph Rupp." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2020. http://d-nb.info/1221960563/34.
Full textKnust, Elisabeth, João Firmino, and Jean-Yves Tinevez. "Crumbs Affects Protein Dynamics In Anterior Regions Of The Developing Drosophila Embryo." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-191623.
Full textChennevière, Alexis. "Dynamique de chaînes de polymère greffés et glissement aux interfaces." Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112404/document.
Full textIn many cases, the development of surfaces with specific adhesive properties involves the use of "decorated interfaces." These interfaces consist of a solid substrate on which polymer chains are more or less well anchored. These chains are mechanically coupled to the surrounding material and control the transmission of friction and adhesion stresses at the interfaces. This coupling depends particularly on the penetration of the surface chains within the matrix and on their own dynamics. In this thesis, the systems we investigated are composed of a layer of polymer chains whose end is covalently linked to a solid substrate. These, so called, polymer brushes, provide a model system for decorated interfaces. Our objective was to study the conformation and dynamics of these grafted chains when they are subjected to different types of stress in order to understand the molecular mechanisms governing the adhesion and friction properties of this type of interface.In the first part, we investigated the healing kinetics of an interface composed initially of grafted chains collapsed on a substrate and in contact with a molten by using neutron reflectivity. When the system is brought above the glass transition temperature, the polymer chains mobility is high enough to allow the penetration of the grafted chains within the polymer melt. Neutrons reflectivity allowed us to probe at the molecular scale and to quantify the healing kinetics of this type of interface. The influence of molecular parameters on this healing kinetics was observed, which allowed us to propose a scaling law model to give a physical interpretation to the phenomenon studied.The second part of this thesis consisted in the development of an experimental setup which is able to shear a brush / melt interface above the glass transition temperature and to freeze the conformation of chains grafted in their sheared conformation. The inversion of the associated neutron reflectivity spectra made it possible to demonstrate the influence of shear on the degree of interpenetration between the brush and the melt which governs the transmission of stresses. In addition, we measured the kinetics of relaxation of grafted chains previously sheared and we compared it to the interdigitation experiments. This comparison highlighted the influence of the kind of solicitation on the relaxation kinetics of a brush/melt interface.We also observed that the relaxation kinetics and the conformation of the grafted chains may be altered when they are confined in a film which thickness is comparable to the radius of gyration of the chains. A systematic study using neutron reflectivity was conducted and highlighted an acceleration of the relaxation kinetics of the system below a critical thickness which could be interpreted in terms of a shift in the glass transition temperature.Secondly, we studied the slip of polymer solutions onto a grafted surface. The volume fraction of free chains in solution is an additional parameter which controls the degree of interpenetration between free chains and grafted chains. A first theoretical approach showed that different slip regimes can occur as a function of volume fraction. We have undertaken a first series of experiments using laser velocimetry after photobleaching to measure the surface velocity of flowing polymer solutions and to compare the experimental results to our theoretical approach
Knust, Elisabeth, João Firmino, and Jean-Yves Tinevez. "Crumbs Affects Protein Dynamics In Anterior Regions Of The Developing Drosophila Embryo." Public Library of Science, 2013. https://tud.qucosa.de/id/qucosa%3A29137.
Full textInthavong, Walailuk. "Elaboration et caractérisation de nanoparticules de protéines." Thesis, Le Mans, 2018. http://www.theses.fr/2018LEMA1014/document.
Full textPolydisperse fractal aggregates of varying average sizes were formed when solutions of whey protein isolate and soy protein isolate were heated at different protein concentrations and at neutral pH. The structure of these fractals aggregates solutions was analyzed by light scattering as a function of protein concentration. In dense suspension, the osmotic compressibility and the correlation length decreases with increasing concentration and become independent of the initial aggregate size. In this concentration regime, the aggregates are strongly interpenetrated and can be visualized as a set of "blobs". For a fixed aggregate size, the viscosity initially increases exponentially with increasing concentration and then diverges at the gel point. Larger fractal aggregates show a more important increase of the viscosity with increasing concentration than smaller aggregates, because they are less dense. The increase of the viscosity was much stronger for large fractal aggregates than for homogeneous microgels (microgels were formed by heating the WPI solution in present of CaCl2) of the same size.Dynamic light scattering, rheology and FRAP measurements were performed to investigate mixtures of different type of aggregates of WPI (fractals/fractals, fractals/microgels) and fractals of mixtures of WPI and SPI. Flow measurements were used to characterise the rheological properties of the aggregate suspension whereas Fluorescence recovery after Photobleaching (FRAP) was used to determine the self diffusion of fluorophore-labelled dextrans chains in mixtures over a wide range of concentrations. The results were compared to the concentration dependence of zero shear viscosity, gel stiffness, osmotic compressibility and correlation length. Brownian diffusion of the dextran chains was observed in aggregate suspensions and weak gels formed just above the gel point with a diffusion coefficient that decreased with increasing concentration, but the dependence was weaker than that of the viscosity. At higher concentrations, densely crosslinked gels were formed, which induced a sharp decrease in the mobility of the dextran chains. For these systems, the recovery of fluorescence was logarithmic over time, suggesting an exponential distribution of diffusion coefficients
Irrechukwu, Onyi Nonye. "Role of matrix composition and age in solute diffusion within articular cartilage." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/19699.
Full textCommittee Chair: Levenston, Marc; Committee Member: Garcia, Andres; Committee Member: Koros, William; Committee Member: Sambanis, Athanassios; Committee Member: Temenoff, Johnna; Committee Member: Vidakovic, Brani.
Mirea, Dragoş Alexandru. "Nanophysical analysis to study evolution of vascular and articular inflammatory pathologies." Thesis, Lyon 1, 2011. http://www.theses.fr/2011LYO10352.
Full textAs vascular (VP) and articular (AP) inflammatory pathologies represent nowadays the principal cause of mortality and disability in industrialized countries, the exact causes favoring their occurrence remain still unknown. The present work aimed at proposing new physical methods to detect the early inflammatory stages through recognition of specific markers and to study the structural and mechanical changes undergone by pathological vascular tissues and synovial fluid (SF). Vascular pathologies can be detected through contrasted MRI pictures. In order to improve the capacity of contrast agents to target specific markers they can be antibody-grafted. Atomic Force Microscopy’s mode Force Spectroscopy (AFM-FS) was used to evaluate the affinity between the Fucoidan as a new antibody, and the P-Selectin vascular inflammatory marker, for capacity to target that marker. Further study of VP used the FS techniques for nanoindentation to study changes in mechanical properties between healthy and pathological vascular tissues. Modifications in SF’s dynamics triggered by one of the molecular component not fulfilling its role may lead to AP. To investigate this issue, each of the main SF’s molecular components had their affinity tested versus the ever-present lipid bilayers using AFM-FS techniques. Furthermore changes in lipid bilayers’ dynamics in the presence/absence of the main SF components were analyzed by Fluorescence Recovery After Photobleaching technique. Finally a tribological test was performed to study the variation of the friction coefficient between the lipid bilayers and SF’s main components
Götz, Anne, and Rolf Jessberger. "Dendritic Cell Podosome Dynamics Does Not Depend on the F-actin Regulator SWAP-70." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-127216.
Full textGötz, Anne, and Rolf Jessberger. "Dendritic Cell Podosome Dynamics Does Not Depend on the F-actin Regulator SWAP-70." Public Library of Science, 2013. https://tud.qucosa.de/id/qucosa%3A27287.
Full textOrgan, Louise E. "Investigating the lateral mobility of outer hair cell plasma membrane constituents by fluorescence recovery after photobleaching." Thesis, 2008. http://hdl.handle.net/1911/22165.
Full textKrieghoff-Henning, Eva [Verfasser]. "Analyzing the regulation of the subcellular localization of β-catenin [beta-catenin] by fluorescence recovery after photobleaching / vorgelegt von Eva Isabelle Krieghoff." 2006. http://d-nb.info/982361378/34.
Full text(10711971), Alex M. Sherman. "Dynamic Chemical Imaging And Analysis Within Biologically Active Materials." Thesis, 2021.
Find full text(11173365), Youlin Liu. "MACHINE LEARNING METHODS FOR SPECTRAL ANALYSIS." Thesis, 2021.
Find full textmaking in measurement sciences, and this process is automated for the liberation of labor. In light of the adversarial approaches shown in digital image processing, Chapter 2 demonstrate how the same attack is possible with spectroscopic data. Chapter 3 takes the question presented in Chapter 2 and optimized the classifier through an iterative approach. The optimized LDA was cross-validated and compared with other standard chemometrics methods, the application was extended to bi-distribution mineral Raman data. Chapter 4 focused on a novel Artificial Neural Network structure design with diffusion measurements; the architecture was tested both with simulated dataset and experimental dataset. Chapter 5 presents the construction of a novel infrared hyperspectral microscope for complex chemical compound classification, with detailed discussion in the segmentation of the images and choice of a classifier to choose.