Dissertations / Theses on the topic 'FLUORESCENCE CHEMOSENSORS'
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Cabell, Larry Allen. "Chemosensors and competition sensing assays /." Digital version accessible at:, 1999. http://wwwlib.umi.com/cr/utexas/main.
Full textJin, Shan. "Development of Boronic Acid-Based Chemosensors." Digital Archive @ GSU, 2009. http://digitalarchive.gsu.edu/chemistry_diss/31.
Full textWilliams, James Anthony Gareth. "Luminescence behaviour of macrocycle metal complexes." Thesis, Durham University, 1995. http://etheses.dur.ac.uk/5313/.
Full textFan, Li-juan. "Design, synthesis and photophysics of fluorescence "turn-on" conjugated polymer chemosensors." Diss., Online access via UMI:, 2006.
Find full textSargenti, Azzurra <1986>. "Study of Magnesium Homeostasis and Intracellular Compartmentalization in Human Cells by Fluorescent Chemosensors and Synchrotron X-Ray Fluorescence." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amsdottorato.unibo.it/7496/1/Sargenti_Azzurra_tesi.pdf.
Full textSargenti, Azzurra <1986>. "Study of Magnesium Homeostasis and Intracellular Compartmentalization in Human Cells by Fluorescent Chemosensors and Synchrotron X-Ray Fluorescence." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amsdottorato.unibo.it/7496/.
Full textFinkelmeier, Nils. "Synthesis and Fluorescence Properties of Anthracene Derivatives and their Metal Complexes." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2013. http://hdl.handle.net/11858/00-1735-0000-0023-994A-7.
Full textPaul, Uchenna Prince. "Fluorescence Detectors for Proteins and Toxic Heavy Metals." Diss., CLICK HERE for online access, 2004. http://contentdm.lib.byu.edu/ETD/image/etd416.pdf.
Full textAkdeniz, Ali. "Sensing of Enantiomeric Excess in Chiral Carboxylates." Bowling Green State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1465223828.
Full textFang, Albert Geeson. "Development of novel fluorescent chemosensors /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2004. http://wwwlib.umi.com/cr/ucsd/fullcit?p3138822.
Full textZhu, Jianfa. "Fluorescent chemosensor development based on multifunctional spirobenzopyrans." HKBU Institutional Repository, 2011. https://repository.hkbu.edu.hk/etd_ra/1299.
Full textPancholi, Jessica. "Novel 'click' generated sensors and molecular machines for fluorescent sensing of Zn2+." Thesis, Queen Mary, University of London, 2015. http://qmro.qmul.ac.uk/xmlui/handle/123456789/15017.
Full textTurkewitsch, Petra. "The synthesis of fluorescent chemosensors responsive tocAMP and other nucleotides." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0018/NQ44611.pdf.
Full textYou, Qihua. "Development of fluorescent chemosensors based on different signal transduction mechanisms." HKBU Institutional Repository, 2014. https://repository.hkbu.edu.hk/etd_oa/95.
Full textTurkewitsch, Petra. "The synthesis of fluorescent chemosensors responsive to cAMP and other nucleotides /." Thesis, McGill University, 1998. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=37551.
Full textTwo new fluorescent molecules, known as 4-(p-dimethylaminostyryl)pyridinium salts or dyes, were synthesized as possible fluorescent components of a chemosensor. Upon excitation at 469 run, dye 1, trans-4-( p-N,N-dimethylaminostyryl)-N-vinylbenzyl-pyridinium chloride, displays a dramatic quantum yield enhancement in an emission band centered at ∼600 nm, with concomitant slight red shift of the emission maximum, in the presence of the cyclic nucleotides, cAMP and cGMP, in aqueous solution. Other purine nucleotides (AMP, ADP and ATP) and adenosine induce fluorescence quantum yield enhancements of lesser magnitude than those observed for cyclic nucleotides. The pyrimidine nucleotides, CMP and UMP, have almost no effect on the fluorescence of 1, suggesting a specificity of 1 for purine over pyrimidine analytes. Equilibrium association constants for 1 with the purine analytes, in aqueous solution (pH 7.2) range from 13.9 M --1 for cAMP to 0.15 M--1 for adenine. We conclude that the interaction of 1 with these analytes requires the presence of a purine base, and is enhanced by the presence of ribose and phosphate moieties. Dye 1 and a structurally-similar dye 2, trans-4-(p-N,N-dimethylaminostryl)-N-phenethylpyridinium bromide, also display dramatic fluorescence enhancements in the presence of DNA and proteins, suggesting that they also interact with these biomolecules. The environmentally-sensitive fluorescence of dyes 1 and 2 suggests that such compounds may be useful for developing fluorescent chemosensors for purine nucleotides, especially cAMP, and for the fluorescence detection or staining of DNA and proteins.
To increase the recognition ability of 1 for cAMP, we prepared recognition sites for cAMP that contain fluorescent dye 1 in a polymer matrix by molecular imprinting. This is a novel design for such template-selective sites, since dye 1 forms an integral part of the recognition cavity, thereby serving as both the recognition element and the measuring element for the fluorescence detection of cAMP in aqueous media. The polymer displays a concentration-dependent decrease in fluorescence in the presence of aqueous cAMP, whereas almost no effect is observed in the presence of the structurally-similar molecule, cGMP. An association constant of ∼105 M--1 was calculated for cAMP binding. Such fluorescent molecularly imprinted polymers could serve as a starting point in the development of highly effective synthetic fluorescent sensors for cAMP as well as other important biological molecules.
Wang, Hao. "Development of fluorescent chemosensors : mercury sensing and biological molecules sensing probes." HKBU Institutional Repository, 2008. http://repository.hkbu.edu.hk/etd_ra/890.
Full textPIERSANTI, LUCA. "Nuovi chemosensori ottici aventi un derivato benzossazolico come unità sensibile." Doctoral thesis, Urbino, 2015. http://hdl.handle.net/11576/2628889.
Full textNolan, Elizabeth M. (Elizabeth Marie) 1978. "Fluorescent chemosensors for exploring zinc metalloneurochemistry and detecting mercury in aqueous solution." Thesis, Massachusetts Institute of Technology, 2006. http://hdl.handle.net/1721.1/36266.
Full textVita.
Includes bibliographical references.
Chapter 1. An Introduction to Zinc Metalloneurochemistry and Zinc Detection in Biology. This chapter presents an overview of zinc neurophysiology and pathology, which provides motivation for the design of new tools and tactics for zinc detection in vivo. A historical account of biological zinc detection is also given, followed by a summary of recent progress in the development and use of fluorescent Zn(II) sensors for in vivo studies. A summary of project goals and thesis organization is also included. Chapter 2. Zinc Sensors Based on Monosubstituted Fluorescein Platforms I: Routes to Electronic Variation, Syntheses and Spectroscopic Characterization. In this work, a convergent synthetic approach for the assembly of fluorescent zinc sensors from aniline-derivatized ligands and a fluroescein carboxaldehyde platform is presented. These sensors are based on the previously reported ZP4 motif and incorporate a di(2-picolyl)amine moiety in the aniline-based ligand framework. The effects of electronic variation, achieved by halogenation of either the zinc-binding unit or the fluorophore platform, on the fluorescence properties and aniline nitrogen atom pKa values are considered.
(cont.) Chapter 3. Spectroscopic Characterization and Biological Applications of Halogenated Zinpyr Sensors. In this chapter, the effects of fluorescein halogenation on the photophysical properties and protonation equilbria of symmetrical Zinpyr derivatives, which contain two di(2-picolyl)amine-based ligand appendages, are considered. These sensors have sub-nM affinity for Zn(II) and are selective for Zn(II) over biologically relevant alkali and alkaline earth metals. Fluorescein halogenation influences background fluorescence, dynamic range, tertiary amine pKa, and both excitation and emission wavelengths. Extensive biological work, including cytotoxicity assays and confocal imaging, are also presented. Studies in a number of cell lines, including neurons, show that ZP3 is a versatile Zn(II) imaging tool. Chapter 4. Zinc Sensors Based on Monosubstituted Fluorescein Platforms II: Modulation of Zinc Affinity and Biological Applications. To access ZP sensors with lower Zn(II) affinity, pyrrole moieties were incorporated into an aniline-based ligand unit to give sensors ZP9 and ZP10. The photophysical characterization and metal binding properties of these sensors are described.
(cont.) The pyrrole-for-pyridyl substitution affords Zn(II) sensors with improved Zn(II) selectivity and sub-[M dissociation constants. Biological imaging studies revealed that asymmetrical ZP probes, including ZP4, are cell permeable and Zn(II) responsive in vivo. Both ZP4 and ZP9 detect endogenous Zn(II) in acute hippocampal slices from the adult rat. Chapter 5. The Zinspy Family of Fluorescent Zinc Sensors: Syntheses and Spectroscopic Investigations. Four fluorescent sensors designed for Zn(II) detection and which contain a fluorescein reporting group and a pyridyl-amine-thioether derivatized ligand moiety were prepared and their photophyiscal properties characterized. These "Zinspy" sensors are water soluble and generally display -1.4 to -4.5-fold fluorescence enhancement upon Zn(II) coordination, depending upon fluorescein halogenation and the number and nature of the Zn(II)-binding appendages. The Zinspy sensors exhibit improved selectivity and lower affinity for Zn(II) compared to the di(2-picolyl)amine-based Zinpyr family members. Chapter 6. Zinspy Sensors with Enhanced Dynamic Range: Imaging Zinc Uptake and Mobilization with a Low Affinity Probe.
(cont.) This chapter describes the preparation and characterization of Zinspy sensors containing non-coordinating thiophene heterocycles in the metal-binding unit. These probes show improved dynamic range relative to thioether-containing ZS sensors, low M dissociation constants for Zn(II) and improved Zn(II) selectivity. Stopped-flow kinetics investigations indicate fast association rates and reversible Zn(II) coordination with kon > 1.8 x 106 M'-s- and koff > 3 s-1 at 25 C. ZS5 is cell permeable, Zn(II)-responsive in vivo and localizes to the mitochondria of certain cell types. ZS5 can detect Zn(II) released from neurons following nitrosative stress. Chapter 7. QZ1 and QZ2, Rapid Reversible Quinoline Derivatized Fluoresceins for Sensing Biological Zinc. Two fluorescein-based dyes derivatized with 8-aminoquinoline were prepared and their photophysical, thermodynamic and zinc-binding kinetic properties determined. Because of their low background fluorescence and highly emissive Zn(II) complexes, QZ1 and QZ2 display a large dynamic range, with -42- and -150-fold fluorescence enhancements upon Zn(II) coordination, respectively.
(cont.) These sensors have micromolar dissociation constants for Zn(II), improved selectivity and bind Zn(II) rapidly and reversibly with kon values >106 M-'s-1 and koffvalues of ~150 s-1. Biological imaging studies with ZP3 and QZ2 show that binding affinity is an important parameter for metal ion detection in vivo. QZ1 and QZ2 also respond to two-photon excitation and two-photon microscopy was used to visualize Zn(II) with QZ2 in live HeLa cells. Chapter 8. A "Turn-On" Fluorescent Sensor for the Selective Detection of Mercuric Ion in Aqueous Media. This chapter describes the synthesis, photophysical characterization and metal-binding properties of mercury sensor 1 (MS1). This sensor is based on a fluorescein platform and has a thioether-rich metal-binding unit, which conveys high selectivity for Hg(II). To the best of our knowledge, MS1 was the first reversible fluorescent Hg(II) sensor to give fluorescence turn-on in water. MS1 can detect low ppb levels of Hg(II) in aqueous solution at neutral pH. Chapter 9. Selective Hg(II) Detection in Aqueous Solution with Thiol Derivatized Fluoresceins. The syntheses and photophysical properties of MS2 and MS3, two asymmetrically derivatized fluorescein-based dyes designed for Hg(II) detection, are described.
(cont.) These sensors each contain a single pyridyl-amine-thiol metal-binding moiety, form 1:1 complexes with Hg(II) and exhibit selectivity for Hg(II) over other Group 12 metals, alkali and alkaline earth metals, and most divalent first-row transition metals. Both dyes display superior brightness ( x ) and fluorescence enhancement following Hg(II) coordination in aqueous solution. At neutral pH, electron transfer (PET) quenching of the free dye is enhanced, and the Hg(II)-induced turn-on also benefits from alleviation of this pathway. MS2 can detect ppb levels of Hg(II) in aqueous solution, demonstrating its ability to identify environmentally relevant concentrations of Hg(II). Chapter 10. MS4, A Seminaphthofluorescein-Based Chemosensor for the Ratiometric Detection of Hg(II). The synthesis and photophysical characterization of MS4, an aniline-derivatized seminaphthofluorescein-based dye that contains a pyridyl-amine-thioether ligand analogous to that employed in the Zinspy Zn(II) sensor family (Chapter 5) are reported. Sensor MS4 provides single-excitation, dual-emission ratiometric detection of Hg(II) in aqueous solution. An -4-fold ratiometric change (624/524) is observed upon introduction of Hg(II) to an aqueous chloride-containing solution of MS4 at pH 8.
(cont.) In this milieu, MS4 shows selectivity for Hg(II) over a background of alkali and alkaline earth metals, a number of divalent first-row transition metals and its Groupl2 congeners Zn(II) and Cd(II). Chapter 11. Turn-On and Ratiometric Mercury Sensing in Water with a Seminaphthofluorescein-Based Probe. The synthesis and characterization of MS5 are presented in this chapter. This sensor incorporates the aniline-derivatized thioether ligand used in the preparation of MS1 and the seminaphthofluorescein platform described in the design of MS4. MS5 gives selective fluorescence turn-on for Hg(II) at pH > 7. At pH > 8, single-excitation dual-emission ratiometric Hg(II) detection is possible by comparison of the (624 / 524) ratio before and after Hg(II) coordination. Studies of the pH dependence suggest that the seminaphthofluorescein dianion is critical for generating the ratiometric response. X-ray crystallographic studies with a salicylaldehyde-based model complex are presented to help elucidate the nature of Hg(II) coordination to MS1 and MS5. MS5 can respond to Hg(II) added to natural water samples, which points to its potential utility in the field.
(cont.) Appendix 1. Miscellenous Fluorescein-Based Ligands. This appendix details the preparation and, in some cases, characterization of potential fluorescein based sensors for either Zn(II) or Hg(II) detection that were not described in earlier chapters. Many of these compounds give fluorescence turn-off or no fluorescence change with analyte binding. Sensors ZP11 and MS6 are lower-affinity probes that give fluorescence turn-on for Zn(II) and Hg(II), respectively. Appendix 2. Theoretical Investigations of Fluorescein Derivatives. In this chapter, we present the results from DFT and TDDFT calculations on fluorescein and its derivatives. These studies include establishing protocols for fluorescein pKa determination and TDDFT analysis. The absorption spectra of the fluorescein dianion and monoanion were assigned and a detailed molecular orbital analysis for a fluorescein dianion analog was conducted. These studies indicate that oxygen atoms in the xanthenone moiety influence the amount of C1 character in the donor and acceptor molecular orbitals responsible for fluorescein absorption.
by Elizabeth M. Nolan.
Ph.D.
Vance, David Howard. "Convergency in the synthesis of anthracene-based fluorescent chemosensors and Dephosphorylation reagents /." The Ohio State University, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487847309050039.
Full textKutuk, Ilker. "Design And Synthesis Of Near-ir Emitting Fluorescent Chemosensors For Transition Metal Ions." Master's thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/12609561/index.pdf.
Full textMello, Jesse Vincent. "Biarylpyridine fluorescent chemosensors : synthesis, structural studies, and combinatorial discovery of a Hg(II) sensor /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2004. http://wwwlib.umi.com/cr/ucsd/fullcit?p3129954.
Full textKaur, Amandeep. "Fluorescent tools for imaging oxidative stress in biology." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/15063.
Full textLaughlin, Sarah R. "Arylboronic Acids With Strong Fluorescence Intensity Changes Upon Sugar Binding." Digital Archive @ GSU, 2011. http://digitalarchive.gsu.edu/chemistry_theses/46.
Full textMarques, Elisabete De Jesus Oliveira. "New fluorescent chemosensors based on bio-inspired ligands and macrocycles: from single molecules to nanoparticles." Doctoral thesis, Faculdade de Ciências e Tecnologia, 2010. http://hdl.handle.net/10362/5386.
Full textProject PTDC/QUI/66250/2006. Project VICOUK914122P64702. Project 09CSA04338PR (in Biomedicine)
Lim, Soojin. "Fluorescent Indicators for Disease Biomarkers." PDXScholar, 2012. https://pdxscholar.library.pdx.edu/open_access_etds/262.
Full textKurishita, Yasutaka. "Development of Molecular Tools for Analysis and Imaging of ATP and Other Biomolecules Based on Coordination Chemistry." 京都大学 (Kyoto University), 2014. http://hdl.handle.net/2433/188614.
Full textGuliyev, Ruslan. "Rational Design Of Ratiometric Chemosensor Via Modulation Of Energy Donor Efficiency." Master's thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/12609889/index.pdf.
Full textBantinaki, Eleni. "Characterisation of a novel chemosensory pathway underlying adaptive evolution in experimental populations of Pseudomonas fluorescens SBW25." Thesis, University of Oxford, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393362.
Full textPedras, Bruno André Cunha de Vallêra Jacques. "Synthesis, characterization and applications of new schiff base fluorescent chemosensors for metal and DNA interactions: conventional and "green" approaches." Doctoral thesis, Faculdade de Ciências e Tecnologia, 2011. http://hdl.handle.net/10362/9684.
Full textThe work presented in this thesis, which has as its central subject the synthesis and applications of new fluorescent chemosensors and probes, can be divided in two major groups. The first one deals with Schiff base-derived fluorescent chemosensors, with application in the detection of metal cations, and the second group concerns the development of new Ru(II) polypyridyl luminescent molecular probes for DNA. Before the description and discussion of the performed work, a general introduction is presented in Chapter I, where emphasis is put on the applications of molecular fluorescence to chemical sensing, in order to obtain a better understanding of the photophysical processes that underlie molecular recognition. This connection is made by defining concepts and giving examples when necessary. A brief mention to the studied analytes and the instrumental methods used both in developing the chemosensors and in their applications is also made. In Chapter II, two new chemosensors possessing crown ether moieties linked through a Schiff base to a bithiophene group are presented. These systems were synthesized and fully characterized, and their application in the sensing of Ni(II), Pd(II), Hg(II) and Na(I) was tested by absorption and emission spectroscopies, and MALDI-TOF MS. Chapter III describes two new pincer-type ligands, in which a thiophene unit is linked through a Schiff base either to pyrene or naphthalene chromophores. Sensing applications for Ni(II) and Pd(II) were investigated by different techniques, and solid complexes with the aforementioned metals were also synthesized. A new tripodal ligand derived from indole and containing three Schiff base groups is introduced in Chapter IV, and its complexes with Zn(II), Cu(II), Ni(II), Hg(II) and Pd(II) are also described. Metal ion titrations of the free ligand with the respective metal salts have been performed, helped by DFT calculations, in order to investigate the role of the central tertiary amine in metal coordination. The search for new luminescent molecular probes to be used in DNA binding is the subject of Chapter V, in which a new family of six Ru(II) polypyridyl complexes is synthesized. The relation between their structure and the binding mode to DNA is studied by absorption and steady-state emission spectroscopies, as well as by timeresolved experiments.
Fundação para a Ciência e a Tecnologia - bolsa de doutoramento SFRH/BD/27786/2006
Chiu, Sheng-Wen. "Spatiotemporal dynamics of cytoskeletal and chemosensory proteins in the bacterium Rhodobacter sphaeroides." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:d7d05b1a-07c5-4e26-9650-37bcfae2fade.
Full textAtilgan, Serdar. "A Sensitive And Selective Ratiometric Near Ir Fluorescent Probes For Zinc And Mercury Ions Based On The Distyryl-bodipy Fluorophore." Phd thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/3/12610842/index.pdf.
Full textOcta-Smolin, Frescilia [Verfasser], and Jochen [Akademischer Betreuer] Niemeyer. "1,1‘-Binaphthyl Based Bis- and Tris-Phosphoric Acids : Syntheses and Application as Fluorescent Chemosensors / Frescilia Octa-Smolin ; Betreuer: Jochen Niemeyer." Duisburg, 2018. http://d-nb.info/117454385X/34.
Full textShi, Danxin. "Preliminary research toward the total synthesis of a novel crown ether that is a potential fluorescent chemosensor for potassium ion recognition." Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/941374.
Full textDepartment of Chemistry
Lo, Presti Maria. "Optical detection of chemical species of environmental and biological relevance using molecular sensors and hybrid materials." Doctoral thesis, Universitat Politècnica de València, 2021. http://hdl.handle.net/10251/172664.
Full text[CA] La present tesi doctoral titulada "Detecció òptica d'espècies químiques d'importància ambiental i biològica utilitzant sensors moleculars i materials híbrids" se centra en el disseny, preparació, caracterització i avaluació de sensors químics moleculars. El treball realitzat es pot dividir en dues parts: (i) síntesi de sensors de cations metàl·lics en dissolució i (ii) síntesi i caracterització de nanopartícules híbrides orgànic-inorgànics per al reconeixement d'espècies químiques i biològiques. En el primer capítol s'introdueix el marc en el qual s'engloben els fonaments teòrics de la química supramolecular en què es basen els estudis pràctics realitzats durant la present tesi doctoral. A continuació, en el capítol dos, es presenten els objectius generals de la tesi. En el tercer capítol es presenta un quimiodosímetro crom-fluorogénic, capaç de detectar selectivament cations trivalents entre cations i anions monovalents i divalents mitjançant una reacció de deshidratació en aigua. En el quart capítol es presenta una unitat (BODIPY) connectada electrònicament amb un macrocicle dithia-dioxa-aza. Les solucions de acetonitril i aigua-acetonitril 95:5 v/v de la sonda van mostrar una banda ICT a la zona visible i són gairebé no emisivas. Quan es va utilitzar acetonitril com a dissolvent, l'addició de Hg (II) i cations metàl·lics trivalents va induir un canvi hipsocròmic de la banda d'absorció i millores moderades de l'emissió. Es va obtenir una resposta altament selectiva a l'utilitzar mitjans competitius com aigua-acetonitril 95:5 v/v. En aquest cas, només el Hg (II) va induir un canvi hipsocròmic de la banda d'absorció i una millora marcada de l'emissió. El cinquè capítol explora el desenvolupament de sensors per berberina i amantadina. Dues molècules d'interès biològic pel seu ús com a fàrmacs. S'han preparat tres sistemes de sensors basats en l'aproximació de portes moleculars. En concret, sobre nanopartícules MCM-41 carregades amb rodamina B com a unitat de senyalització, s'ha dut a terme la funcionalització amb diverses amines i el bloqueig de porus amb cucurbituril CB7. Les amines utilitzades són ciclohexilamina, bencilamina i amantadina. Els materials obtinguts s'han caracteritzat per tècniques iv de difracció de raigs X i microscòpia electrònica de transmissió confirmant l'estructura mesoporosa de les nanopartícules. Els materials preparats mostren una resposta a la berberina i la adamantina, llevant el tap i alliberant el tint fluorescent a l'mig. La resposta dels materials a les dues substàncies d'interès (berberina i amantadina) depèn de l'estructura química de cada un dels materials en funció de les constants d'afinitat entre l'anàlit i CB7. Els resultats obtinguts obren el camí a l'ús de portes moleculars com a sensors de berberina i amantadina.
[EN] The present doctoral thesis entitled "Optical detection of chemical species of environmental and biological importance using molecular sensors and hybrid materials" focuses on the design, preparation, characterization and evaluation of molecular chemical sensors. The work carried out can be divided into two parts: (i) synthesis of metal cation sensors in solution and (ii) synthesis and characterization of hybrid organic-inorganic nanoparticles for the recognition of chemical and biological species. The first chapter introduces the framework that encompasses the theoretical foundations of supramolecular chemistry on which the practical studies carried out during this doctoral thesis are based. Next, in chapter two, the general objectives of the thesis are presented. In the third chapter, a chromium-fluorogenic chemodosimeter is presented, capable of selectively detecting trivalent cations by means of a dehydration reaction in water. The fourth chapter presents a new compound containing a BODIPY unit electronically connected with a dithia-dioxa-aza macrocycle. Acetonitrile and water-acetonitrile 95:5 v/v solutions of the probe showed an ICT band in the visible zone and were nearly non-emissive. When acetonitrile was used as a solvent, addition of Hg(II) and trivalent metal cations induced an hypsochromic shift of the absorption band and moderate emission enhancements. A highly selective response was obtained when using competitive media such as water- acetonitrile 95:5 v/v. In this case only Hg(II) induced a hypsochromic shift of the absorption band and a marked emission enhancement. The fifth chapter explores the development of sensors for berberine and amantadine; two molecules of biological interest due to their use as drugs. Three sensing systems based on a "molecular gate" approximation have been prepared. Specifically, MCM-41 nanoparticles were loaded with Rhodamine B as a signalling unit, functionalized with various amines and capped with cucurbituril CB7. The amines used are cyclohexylamine, benzylamine and amantadine., The materials obtained were characterized by X-ray diffraction techniques and transmission vi electron microscopy, confirming the mesoporous structure of the nanoparticles. The prepared materials showed a response to berberine and adamantine, which induced release of the fluorescent dye to the medium. The response of the materials to the two substances of interest (berberine and amantadine) depends on the chemical structure of the capping ensemble and it is a function of the affinity constants between the analyte and CB7. The results obtained open the way to the use of gated materials as berberine and amantadine probes.
We thank the Spanish Government (MAT2015-64139-C4-1-R) and Generalitat Valenciana (PROMETEOII/2014/047). M. L. P. thanks Generalitat Valenciana for her Grisolia fellowship. Thanks are also due to Fundação para a Ciência e Tecnologia (Portugal) for financial support to the Portuguese NMR network (PTNMR, Bruker Avance III 400-Univ. Minho), FCT and FEDER (European Fund for Regional Development)-COMPETEQREN- EU for financial support to the research centre CQ/UM [PEst-C/ QUI/UI0686/2013 (FCOMP-01-0124-FEDER-037302)], and a post- doctoral grant to R. M. F. Batista (SFRH/BPD/79333/2011).
Lo Presti, M. (2021). Optical detection of chemical species of environmental and biological relevance using molecular sensors and hybrid materials [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/172664
TESIS
Dubois, Fabien. "Etude des modifications des propriétés spectroscopiques de chemosenseurs fluorescents extrinsèques en présence de cadmium (II), de zinc (II) et de mercure (II)." Doctoral thesis, Universite Libre de Bruxelles, 2002. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/211410.
Full textCheng, Yunfeng. "Development of Boronic Acid Flurescent Reporters, Boronic Acid-Modified Thymidine Triphosphates for Sensor Design and Antagonists of Bacterial Quorum Sensing in Vibrio Harveyi." Digital Archive @ GSU, 2011. http://digitalarchive.gsu.edu/chemistry_diss/58.
Full textOLIVARI, MARTINA. "Neutral receptors for anion recognition: synthesis, selectivity studies and transport properties." Doctoral thesis, Università degli Studi di Cagliari, 2014. http://hdl.handle.net/11584/266429.
Full textHubble, Lee John. "Development of carbon nanotube-based gas and vapour sensors and supramolecular chemistry of carbon nano-materials." University of Western Australia. Centre for Forensic Science, 2009. http://theses.library.uwa.edu.au/adt-WU2009.0191.
Full textSantos, Figueroa Luis Enrique. "New approaches for the development of chromo-fluorogenic sensors for chemical species of biological, industrial and environmental interest." Doctoral thesis, Universitat Politècnica de València, 2015. http://hdl.handle.net/10251/43216.
Full textSantos Figueroa, LE. (2014). New approaches for the development of chromo-fluorogenic sensors for chemical species of biological, industrial and environmental interest [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/43216
TESIS
Premiado
ZARTIT, RANIA. "Synthesis of new macrocyclic and macropolycyclic systems for metal ion recognition. Sintesi di nuovi sistemi macrociclici e polimacrociclici per il riconoscimento di cationi metallici." Doctoral thesis, 2015. http://hdl.handle.net/2158/1043014.
Full textBARTOLI, FRANCESCO. "SYNTHESIS OF NEW POLYAMINE RECEPTORS AND THEIR METAL COMPLEXES AS FLUORESCENCE CHEMOSENSORS FOR ANIONS." Doctoral thesis, 2015. http://hdl.handle.net/2158/1054958.
Full textChang, Wei-Chih, and 張惟智. "Polyethoxy Salicylideneimines as Fluorescence Chemosensors: Design, Synthesis and Sensing in Different Solvent Media." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/90208340860561831140.
Full text國立暨南國際大學
應用化學系
102
Two new fluorescence chemosensors, 1,4-Bis(2-(2-(2-(3,5-di-tert-butyl-salicyald-2-imine)phenoxy)ethoxy)ethoxy)ethoxy)benzene ( named as compound DT ) and 1,4-Bis(2-(2-(2-(3,5-dichloro-salicyald-2-imine)phenoxy)ethoxy)ethoxy)ethoxy)benzene ( named as compound DC ) hereafter, containing polyethoxy chain and salicyaldimine moiety has been designed and synthesized. The cation recognition abilities of DT and DC toward various cations ( Li+, Na+, K+, Cs+, Mg2+, Ca2+, Sr2+, Ba2+, Ag+, Al3+, Cd2+, Co2+, Cr3+, Cu2+, Mn2+, Ni2+, Pb2+ and Zn2+ ) in different solvent media have been examined by UV-Vis and fluorescence spectroscopies. In methanol and 10 mM HEPES media, sensors DT and DC also showed ″Off-On″ fluorescence signaling behavior when interacted with Zn2+ ion. (I) Methanol as media: According to the competitive studies, in the DT-Zn system, Zn2+ ion would be replaced by Al3+, Cr3+, Cu2+ and Pb2+ ions while in the DC-Zn system, Zn2+ ion would be replaced by Co2+, Cr3+, Cu2+, Ni2+ and Pb2+ ions, as observations of absorption change in the UV-vis spectra and fluorescence quenching in the fluorescence spectra. Interestingly, when adding Ba2+ ion into the solution of DT-Zn system and adding Al3+ ion into the solution of DC-Zn system, the fluorescence intensity for both systems are enhanced, signifying noticeable co-operative effect and substitution effect, respectively. On the basis of titration and Job′s plot, sensors DT and DC bind with Zn2+ both in a 2:3 stoichiometry. (II) 10 mM HEPES as media: Sensors DT and DC recognize only the Zn2+ ion. No other metal ion can replace Zn2+ ion in the DT-Zn and DC-Zn complexes.
Chen, Chun-Lin, and 陳群霖. "Colorimetric and Ratiometric Fluorescent Chemosensors for Anions." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/40360733737680328712.
Full text國立中正大學
化學所
94
We have designed and synthesized a series of chemosensors containing amide and pyrrole groups for anion recognition and sensing. The binding affinity between these chemosensors and various anions has been investigated to elucidate the influence of electronic effect generated by different functional groups such as terthiophene, dinitrobenzene, and diphenylquinoxaline in the structure of these chemosensors. According to the changes of UV-Vis and fluorescentce spectra on addition of anions, the chemosensors 1, 2 and 3 display strong binding ability to fluoride, pyrophosphate and cyanide. Moreover, the appearance of the ratiometric phenomenon upon interaction with anions further enhances the spectral differentiation for anion sensing. The reaction mechanism between chemosensor and anions is further investigated by the 1H-NMR titration experiments. Anions strongly intereacting with chemsensors and producing large changes during the titration in UV-Vis and fluorescent spectra usually result in deprotonation of the amide group while the weaker anions initially form hydrogen bonds with NHs on amides and pyrroles followed by deprotonation at higher anion concentration. The weakest anions, however, form only 2 or 4 hydrogen bonds with chemosensors during titration. Moreover, both sensors 2 and 3 are able to recognize cyanide under semi-aqueous environment. We proposed that the formation of covalent bonded cyanohydrin derivatives from cyanide addition to carbonyl center is responsible for the effectiveness of sensors 2 and 3 in semi-aqueous environment. The structures of the cyanide adducts have been confirmed by 1H-NMR and ESI mass spectra. On the other hand, sensor 1 could not form stable cyanide adduct under the competition with water due to the less electron-withdrawing nature of terthiophene. Based on the hydrogen bonding and deprotonation, chemosensors 1, 2 and 3 show very good recognition ability to F- and HP2O73- in polar organic solvents. Most importantly, we have demonstrated that chemosensors 2 and 3 exhibit outstanding selectivity and sensitivity to cyanide over other anions in the semi-aqueous solution, which renders them highy promising chemosensors for cyanide.
Liu, Hao-Ming, and 劉浩敏. "Selective Turn-on Fluorescent Chemosensors for Zn2+." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/83548961019625517788.
Full text國立交通大學
應用化學系碩博士班
100
In this thesis, three chemosensors DP1, DP2 and DP3 based on dipyrrins were designed for Zn2+ detection. DP1 produced significant fluorescence enhancement (20 fold) in the presence of Zn2+ ion, while the metal ions Ag+, Ca2+, Cd2+, Co2+,Cu2+, Fe2+,Fe3+, Hg2+, Mg2+, Mn2+, Ni2+ and Pb2+ produced only minor changes in fluorescence. After addition of more than one equivalent of Zn2+, the emission intensity reached a maximum, at which the quantum yield was 0.10. According to 1H NMR studies, addition of Zn2+ caused the disappearance of amine (NH) hydrogen signal. This observation indicates that Zn2+ binding with DP1 is mainly through one nitrogen atom at benzoimidazole moiety and two nitrogen atoms at the dipyrrin group. The binding ratio of DP1–Zn2+ complexes was determined as 1 : 1 according to Job plot. Using Benesi-Hildebrand plot, the binding constant of DP1–Zn2+ complexes was decided as 6.12 x 104 M-1. The detection limit of the DP1-Zn2+ was 0.236 μM. The quantum yield and the binding constant of the DP2-Zn2+ were 0.017 and 6.64*104 M-1, respectively. The detection limit was 0.325 μM of DP2 for binding Zn2+. The quantum yield and the binding constant of DP3–Zn2+ complexes were 0.026 and 1.47*105 M-1, respectively. The detection limit was 0.492 μM of DP3 for detecting Zn2+.
Huang, Tien-Peng, and 黃天鵬. "Syntheses of Novel Fluorescent Chemosensor: Bis-crown-distyryl naphthalene 6,7,8 and fluorescence changes after chelated with sodium、potassium ions." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/95999521608566585668.
Full textGUO, Chao-Ci, and 郭朝其. "The Synthesis of glycosidase inhibitors and fluorescent chemosensors derivatives." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/58171484089769612463.
Full text國立彰化師範大學
化學系
96
We synthesized a series of N-alkylated aza-C-glycosides starting from the commercial D-ribose. Our strategy take advantage of a direct coupling reaction of alkylated amine with 5-OMs-ribose and further undergoes intramolecular hetero-Michael reaction under base condition. In addition, we also synthesized a novel cyclodextrin-like artifical receptor which is a 1,2,3-triazole compound through Huisgen 1,3-dipolar cycloadditions of alkynes to azides. This idea is based upon the assumption that a cyclic array of carbohydrate moieties, nitrogen atom and amino acid functional groups may lead to exquisite specificity of recognition and catalysis. If compound 9、10、11 with an appropriate appended chromophore would be a good candidate for cation probes because of their aza-crown structures which can be used as a cation recognition unit.
Chen, Chan-Yu, and 陳瞻佑. "Cooperation Chromogenic and Fluorescent Chemosensors for Fluoride and Related Anion." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/46452122000099815432.
Full text國立中央大學
化學研究所
94
We have designed and synthesized two chromogenic and fluorescent chemosensors S1 and S2, which provide hydrazine and hyrdazone groups to form hydrogen bonding interactions with various anions. Through the variations of UV-Vis absorption and fluorescent spectra, the sensing mechanism of compounds S1 and S2 with a variety of anion including F-, Cl- , Br- , I- , CN- , OAc- , and H2PO4- are systematically investigated. According to the results of UV-Vis absorption changes and fluorescence quenching, the binding strength of chemosensors S1 and S2 to different anions can be depicted in the following order: F- > CN- > OAc- > H2PO4- > Cl- > Br-~I-. Due to the electron-withdrawing nature of hydroxyl groups in S1, the hydrogen bonding between hydrazine/hydrazone and anions is stronger compared to S2. Furthermore, Job plots revealed that both sensors S1 and S2 react with anions in a 1:1 stoichiometry. On the other hand, the characteristic band for the excimer could be found from the fluorescent spectra of S1 titrated with F- or CN- . On the basis of these evidences We propose the formation of a [2+2] associated dimer complex via hydrazine/hydrazone-anion hydrogen bonding and ��-�� stacking interaction. The Hill plots and Scatchard plots further confirmed that a [2+2] complex composed of sensor S1 and F- , or CN- formed under the positive coopreativity. In light of obtained information, we propose the stepwise formation mechanism for the interaction between sensor S1 and F- or CN-. Sensor S1 initially form a [2+1] complex with anion followed by a fast dimerization to form the [2+2] associated dimer complex. Moreover, in the case of F-, addition of excess F- could deprotonate the hydroxyl group and generate negative charged [2+2] dimer which disassemble into individual monomer due to the charge repulsion.
Chou, Yu-Shan, and 周郁姍. "Synthesis and Fluorescence Chemosensory Properties of Conjugated Polyfluorenes Containing Diketopyrrolopyrrole Moieties." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/74218118865057352375.
Full text元智大學
化學工程與材料科學學系
104
This study describes the synthesis of conjugated polyfluorene (PF) and conjugated polymer (PFDPP) containing diketopyrrolopyrrole (DPP) groups in the main chain, through Suzuki coupling polymerization. The weight-average molecular weights of PF and PFDPP were 2.18 × 104 and 0.74 × 104 g/mol, with the corresponding polydispersity indices being 2.01 and 1. 47, respectively. PF and PFDPP exhibited the strong UV-vis absorption bands at 380 and 489 nm in the THF solution, respectively. The absorption band of PFDPP was red-shifted by approximately 109 nm relative to PF (380 nm). This red-shift was attributed to the electronic transitions in PFDPP that involved the migration of electron density from fluorene donor groups towards the DPP acceptor, resulting in the observed ICT interactions and bathochromic shifts. PF and PFDPP showed the strong emission bands at 416 nm and 547 nm in THF solution. Photoluminescence titrations demonstrated that PFDPP exhibited high sensitivity towards Fe3+ and F- ion, with Stern-Volmer constants (Ksvs) of 1.58 × 104 M-1 and 1.07 × 103 M-1, respectively. The detection limit (LOD) towards Fe3+ and F- ion was as low as 4.90 × 10-6 M and 1.34 × 10-5 M, respectively. PFDPP showed fluorescence quenching in the pH value of 7~13. The estimated HOMO and LUMO energy levels for PFDPP were –5.70 and –3.46 eV, respectively. These results demonstrated that PFDPP is a promising material for application of sensing cations, anions and pH values.
Walkup, Grant Kingsley. "Fluorescent peptidyl chemosensors for the measurement of divalent metal cation concentrations." Thesis, 1998. https://thesis.library.caltech.edu/7279/2/Walkup_kg_1998.pdf.
Full textStudies toward the production of fluorescent chemosensors for trace divalent metal ions have been conducted, with emphasis placed on the attainment of sufficient analyte selectivity and sensitivity for the measurement of environmental or biomedical samples. One technique that has historically been applied for the design of selective metal ion sensors is to prepare biosensors, devices that exploit proteins for their unmatched specificity in the recognition of small molecules. Alternately, the construction of abiotic chemosensors has been favored by other researchers, but the synthetic simplicity and enhanced durability exhibited by these agents comes at the expense of analyte-detection selectivity. By applying a strategy that is a hybrid of these approaches, selective and sensitive chemosensors for divalent zinc, copper, and nickel have been prepared. These devices combine the advantageous aspects of biosensors within a peptidyl architecture that by virtue of its synthetic origin contains an expanded repertoire of amino acids for metal ion binding and signaling.
Fluorescent chemosensors for Zn(II) have been prepared that are based upon the zinc finger domains and enable the quantitation of sub-micromolar concentrations of that ion in the presence of many other divalent ions. In addition, other fluorosensors have been prepared that employ nonnatural alpha-amino acid derivatives that contain the bidentate metal binding functionality of oxine (8-hydroxyquinoline). These also enable the selective detection of sub-micromolar concentrations of Zn(II), but require only seven amino acid residues as opposed to the 25 present in the zinc finger-based sensors.
By exploiting the metal binding properties of the amino terminal Cu(II)- and Ni(II)-binding (ATCUN) motif of the serum albumins, sensors have been prepared which enable the selective determination of sub-micromolar concentrations of the Cu(II) ion, even in the presence of elevated concentrations of Ni(II). Sensors for Ni(II) that employ a fluorescence resonance energy transer (FRET) mechanism for analyte detection have also been prepared. In addition, studies have been performed to convert these solution based chemosensing reagents into solid phase-attached devices, in order to perform realtime measurements with regenerable sensing materials.
Tsou, Chia-Chen, and 鄒佳臻. "N,N’-diphenylpyridine-2,6-dicarboxamide-based fluorescent chemosensors for sensing anions." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/62497002187402959260.
Full text國立中央大學
化學研究所
93
Two N,N’-diphenylpyridine-2,6-dicarboxamide-based fluorescent chemosensors M1 and M2 were designed and synthesized for sensing anions. The binding affinity of M1 and M2 toward various anions was investigated by UV-Vis and fluorescence spectroscopy. Several anions were surveyed in this research, including hydrogen phosphate, pyrophosphate, halides, cyanide, nitrate, hydrogen sulfate and acetate. Notably, both M1 and M2 are particularly sensitive to anions with tetrahedron geometry, i.e. pyrophosphate and hydrogen phosphate. In general, binding constants of these two anions are higher than other anions with different geometries. The 1:1 binding ratio of the chemosensor-anion complex was determined by Job-plot. A blueshift in fluorescence spectra of M1 and M2 were observed upon titration of with fluoride, pyrophosphate and hydrogen phosphate. It is believed that blueshift was induced by raising the LUMO with the formation of hydrogen bonds between the anions and amide hydrogens. In addition, M2 formed gel-like structure in certain solvents. Some tubular structures were constructed after solvent evaporation, which could be observed by microscopes. The study of the nature and properties of the gel is under progress.