Relevant bibliographies by topics / Flow cytometers

Academic literature on the topic 'Flow cytometers'

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Published: 6 September 2023

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Journal articles on the topic "Flow cytometers"

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Chao, Zixi, Yong Han, Zeheng Jiao, Zheng You, and Jingjing Zhao. "Prism Design for Spectral Flow Cytometry." Micromachines 14, no. 2 (January 26, 2023): 315. http://dx.doi.org/10.3390/mi14020315.

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Flow cytometers are instruments used for the rapid quantitative analysis of cell suspension. Traditional flow cytometry uses multi-channel filters to detect fluorescence, whereas full-spectrum fluorescence based on dispersion detection is a more effective and accurate method. The application of various dispersion schemes in flow cytometry spectroscopy has been studied. From the perspective of modern detectors and demand for the miniaturization of flow cytometry, prism dispersion exhibits higher and more uniform light energy utilization, meaning that it is a more suitable dispersion method for small flow cytometers, such as microfluidic flow cytometers. Prism dispersion designs include the size, number, and placement of prisms. By deducing the formula of the final position of light passing through the prism and combining it with the formula of transmittance, the design criteria of the top angle and the incident angle of the prism in pursuit of the optimum transmittance and dispersion index can be obtained. Considering the case of multiple prisms, under the premise of pursuing a smaller size, the optimal design criteria for dispersion system composed of multiple prisms can be obtained. The design of prism dispersion fluorescence detection was demonstrated with a microfluidic flow cytometer, and the effectiveness of the design results was verified by microsphere experiments and practical biological experiments. This design criterion developed in this study is generally applicable to spectral flow cytometers.
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Volotovski, I. D., and S. V. Pinchuk. "Flow cytometry. Basics of technology and its application in biology." Proceedings of the National Academy of Sciences of Belarus, Biological Series 67, no. 2 (May 4, 2022): 229–42. http://dx.doi.org/10.29235/1029-8940-2022-67-2-229-242.

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The given review is an attempt of concentrated consideration of flow cytometry problem which is widely used as a fundamental research approach in various fields of biology like cell biology, biophysics, biochemistry and molecular biology and also in applied and diagnostic medicine. The method principle, construction of flow cytometers and their possibilities (study of structure and function state of cell populations and cell sorting), usage of lasers in flow cytometers, wide assortment of fluorophores and monoclonal antibodies. The concrete examples of flow cytometer methods in different experiments are given. The trends in development of flow cytometry are considered.
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Cottingham, Katie. "Incredible shrinking flow cytometers." Analytical Chemistry 77, no. 3 (February 2005): 73 A—76 A. http://dx.doi.org/10.1021/ac053330g.

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Anderson, April, and Jolene Bradford. "Rare Event detection using Acoustic Cytometry (144.4)." Journal of Immunology 184, no. 1_Supplement (April 1, 2010): 144.4. http://dx.doi.org/10.4049/jimmunol.184.supp.144.4.

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Abstract Detection of rare events includes populations comprising less than 5% of the total cells, which involves the detection of stem cells, minimal residual disease and fetomaternal hemorrhage. Analysis of rare cell populations requires the collection of high numbers of events in order to attain a reliable measure of accuracy, leading to long acquisition times. Acoustic cytometry is a new technology which allows dilute samples to be processed quickly. We compared collection times of the Applied Biosystems® Attune™ Acoustic Focusing Cytometer to conventional cytometers. Varying numbers of CD34+ KG-1a cells were spiked into whole blood collected from a normal donor, stained with CD45 & CD34 conjugates, and then collected on traditional and acoustic cytometers. Two hundred thousand live cells were acquired and acquisition time recorded, with results compared across all instruments tested. Rare CD34+ populations were detected on all instruments, giving equivalent results. Acquisition time was significantly reduced using an acoustic cytometer compared to a standard flow cytometer. Acquisition times on the acoustic cytometer can be reduced by up to 5-10 fold compared to traditional flow cytometers, while maintaining comparable results in rare event detection. Utilizing the acoustic focusing cytometer rare event acquisition can be accomplished in one fifth of the time without sacrificing data integrity.
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Shrirao, Anil B., Zachary Fritz, Eric M. Novik, Gabriel M. Yarmush, Rene S. Schloss, Jeffrey D. Zahn, and Martin L. Yarmush. "Microfluidic flow cytometry: The role of microfabrication methodologies, performance and functional specification." TECHNOLOGY 06, no. 01 (March 2018): 1–23. http://dx.doi.org/10.1142/s2339547818300019.

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Flow cytometry is an invaluable tool utilized in modern biomedical research and clinical applications requiring high throughput, high resolution particle analysis for cytometric characterization and/or sorting of cells and particles as well as for analyzing results from immunocytometric assays. In recent years, research has focused on developing microfluidic flow cytometers with the motivation of creating smaller, less expensive, simpler, and more autonomous alternatives to conventional flow cytometers. These devices could ideally be highly portable, easy to operate without extensive user training, and utilized for research purposes and/or point-of-care diagnostics especially in limited resource facilities or locations requiring on-site analyses. However, designing a device that fulfills the criteria of high throughput analysis, automation and portability, while not sacrificing performance is not a trivial matter. This review intends to present the current state of the field and provide considerations for further improvement by focusing on the key design components of microfluidic flow cytometers. The recent innovations in particle focusing and detection strategies are detailed and compared. This review outlines performance matrix parameters of flow cytometers that are interdependent with each other, suggesting trade offs in selection based on the requirements of the applications. The ongoing contribution of microfluidics demonstrates that it is a viable technology to advance the current state of flow cytometry and develop automated, easy to operate and cost-effective flow cytometers.
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Shapiro, Howard M., and Nancy G. Perlmutter. "Personal cytometers: Slow flow or no flow?" Cytometry Part A 69A, no. 7 (2006): 620–30. http://dx.doi.org/10.1002/cyto.a.20284.

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NOGUCHI, Yoshio, and Yoshio TENJIN. "Laser flow cytometers newly developed." Review of Laser Engineering 15, no. 8 (1987): 647–56. http://dx.doi.org/10.2184/lsj.15.647.

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Nitta, Carolina Franco, Mackenzie Pierce, Jeanne Elia, Jen Ruiz, Art-Danniel Hipol, Nicolas Fong, Henry Qazi, et al. "A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca PLX image cytometer." Journal of Immunology 210, no. 1_Supplement (May 1, 2023): 250.06. http://dx.doi.org/10.4049/jimmunol.210.supp.250.06.

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Abstract Cellular therapies such as chimeric antigen receptor (CAR) T or NK cells undergo phenotypic characterization during discovery and development of novel therapies, biomanufacturing, and upstream patient cell processing for clinical use. Samples are routinely analyzed via flow cytometry for T cell, NK cell, B cell, and monocyte surface marker expression. Image cytometry systems perform cell-based assays that can be used as a convenient alternative to flow cytometry. Recently, a new high-throughput image cytometer, the Cellaca PLX system (Nexcelom, Lawrence, MA) was developed for immunophenotyping, transfection/transduction efficiency, and cell health assays. This new instrument can assess several critical quality attributes (CQAs) such as cell identity, viability, and other relevant biological functions recommended by the International Organization for Standardization using the ISO Cell Characterization that focuses on cellular therapeutic products. Here we demonstrate a rapid and high-throughput immunophenotyping and viability detection in peripheral blood mononuclear cells (PBMCs) using the Cellaca PLX. PBMCs underwent red blood cell (RBC) lysis and CD3 enrichment, were stained with Hoechst/CD3/CD4/CD8 or Hoechst/CD3/CD8/RubyDead surface marker kits and measured on the Cellaca PLX and three different flow cytometers for comparison. Results showed highly comparable cell populations between the flow cytometers and the Cellaca PLX suggesting that the image cytometer may provide a rapid and convenient alternative method for immunophenotyping. The proposed method can streamline workflows from sample staining to data analysis, quickly moving precious patient samples into downstream processes.
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Kotz, Kenneth T., Anne C. Petrofsky, Ramin Haghgooie, Robert Granier, Mehmet Toner, and Ronald G. Tompkins. "Inertial focusing cytometer with integrated optics for particle characterization." TECHNOLOGY 01, no. 01 (September 2013): 27–36. http://dx.doi.org/10.1142/s233954781350009x.

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Microfluidic inertial focusing has been shown as a simple and effective method to localize cells and particles within a flow cell for interrogation by an external optical system. To enable portable point of care optical cytometry, however, requires a reduction in the complexity of the large optical systems that are used in standard flow cytometers. Here, we present a new design that incorporates optical waveguides and focusing elements with an inertial focusing flow cell to make a compact robust cytometer capable of enumerating and discriminating beads, cells, and platelets.
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Bang, Hyunwoo, Hoyoung Yun, Won Gu Lee, Junha Park, Joonmo Lee, Seok Chung, Keunchang Cho, Chanil Chung, Dong-Chul Han, and Jun Keun Chang. "Expansion channel for microchip flow cytometers." Lab on a Chip 6, no. 10 (2006): 1381. http://dx.doi.org/10.1039/b604578b.

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Dissertations / Theses on the topic "Flow cytometers"

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Bausch, Cornelius Sebastian [Verfasser]. "Tubular coplanar waveguides for the use in radio-frequency microfluidic flow cytometers / Cornelius Sebastian Bausch." München : Verlag Dr. Hut, 2016. http://d-nb.info/1103872044/34.

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Le, Lann Lucas. "Elaboration d'une procédure standardisée d'harmonisation des données de cytométrie en flux dans le cadre d'études multicentriques Multi-center harmonization of flow cytometers in the context of the European “PRECISESADS” project, in Autoimmunity Reviews 15(11), November 2016." Thesis, Brest, 2019. http://www.theses.fr/2019BRES0048.

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L'objectif de la thèse est d'assurer la comparabilité des données de cytométrie générées au cours d'une étude multi-centrique.Ce travail de thèse s'inscrit dans le cadre du projet PRECISESADS. Ce projet européen cherche à reclassifier les maladies auto-immunes systémiques en utilisant une approche dite "omique" pour obtenir des signatures biologiques. Cette approche regroupe des outils comme la génomique, la protéomique ... et lacytométrie en flux. Le nombre important d'individus inclus dans le projet rend obligatoire l'utilisation d'outils informatiques pour automatiser l'analyse des milliers de fichiers de cytométrie obtenus au cours des cinq années du projet. Les fréquences des populations cellulaires extraites des fichiers sont comparables entre les centres mais l'intensité médiane de fluorescence (IMF) des molécules étudiées ne l'est pas, malgré une phase de normalisation des données. La cause de cette non-comparabilité est une combinaison d'un effet lot et d'un effet centre. Ces deux effets peuvent être corrigés à l'aide de coefficients spécifiques. La normalisation et la correction des effets lot et centre par l'élaboration de script R et sous python, permettent d'obtenir des IMF comparables entre les centres. Au final, ce travail de thèse a permis d'établir une nouvelle procédure standardisée utilisable dans tous les projets multi-centriques prospectifs d'analyse de données de cytométrie en flux
The aim of this thesis is to ensure the comparability of flow cytometry data within the context of multi-center studies. This thesis work is part of the PRECISESADS project. This European project seeks to reclassify the systemic autoimmune diseases using "omic" data to find useful biological signatures. This encompasses tools like genomic, proteomic ... and flow cytometry. The inclusion of numerous individuals in the project make the use of informatics tools a must for the analysis automation of the thousands flow cytometry files obtained during the 5-years period of the project.Cell populations frequencies extracted from the files are comparable between centers but that is not the case for the median of fluorescence intensities (MFI) of the studied molecules, despite a normalization step. The origin of this incomparability is due to a combination of a batch effect and a center effect. Those two effects can be corrected with specific coefficients. The normalization and correction of both batch and center effect thanks to the elaboration of new R script and python script allow the production of comparable MFI between centers. Overall, this thesis work established a new standardized procedure, efficient for any multi-center projects of flow cytometry data analysis
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Venkatesh, Mukung C. (Mukund Chakravarthy). "Optimization of the mini-flo flow cytometer dc by Mukund C. Venkatesh." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/40009.

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Davey, Hazel Marie. "Flow cytometry of microorganisms." Thesis, Aberystwyth University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309050.

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Novak, John P. (John Peter) 1957. "Development of the in vivo flow cytometer." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/30331.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2004.
Includes bibliographical references.
An in vivo flow cytometer has been developed that allows the real-time detection and quantification of circulating cells containing fluorescent proteins or labeled with fluorochrome molecules in live animals, without the need to extract blood samples. A stationary laser beam is focused by a cylindrical lens to a slit of light that is then demagnified and focused across a blood vessel by an achromat and microscope objective. Fluorescent cells are excited one by one as they flow through the excitation laser light slit, creating a burst of fluorescence whose width is inversely proportional to their velocity. The fluorescence signal is detected through a confocal slit aperture using a photomultiplier tube. The analog signal from the photomultiplier tube is then digitized, filtered, and recorded as a function of time onto a computer. Computer programs post- process the data for the presence of cell signal, as well as various aspects of the cell signal such as height, width, and temporal location of the signal peak. Two in vivo flow cytometers have been built: a single-slit, single-color system and a two- slit, two-color system. The single-slit, single-color system provides excitation at 632 nm, and the two-slit, two-color system provides excitation at 632 nm and 473 nm. The two- slit, two-color system can operate in several different modes: single-slit at 632 nm or 473 nm, double-slit at 632 nm or 473 nm, and double-slit with one excitation slit at 632 nm and the other at 473 nm.
(cont.) Thus far, the single-slit, single-color system has been used to study the circulation kinetics of different prostate cancer and leukemia cell lines with different metastatic potential, as well as the effect of different host environments (i.e., mouse versus rat). In addition, the device has been used to develop a new in vivo labeling method of white blood cells that does not result in significant depletion of the labeled cells, allowing for the possibility of autoimmune and transplant rejection studies. The two-slit, two-color system is being used to track two different cell populations, or one cell population labeled with two different markers, one of which can be the green fluorescent protein.
by John P. Novak.
Ph.D.
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Wållberg, Fredrik. "Flow cytometry for bioprocess control." Licentiate thesis, KTH, Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-1736.

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During bio-technical processing it is important to monitorbiological parameters such as cell growth, viability andproduct formation. Many of the analyses traditionally used areslow to perform and provide only average data for thepopulation. Flow cytometry is a laser-based technique, whichmeasures physical properties of a cell in a flowing stream, ata rate of several thousand cells per second. It offers theprospect of an at-line, multi-parameter analysis of individualmicroorganisms in a population.

In this project several methods for at-line measurements ofbioprocesses were developed such as protocol's for measuringcell concentration, viability and product formation. Theprimary focus was on prokaryotic organisms (E. coli) but eukaryotic organisms (P. pastoris) were included.

The possibility to use volumetric cell counting to measurecell concentration (cell number) was evaluated. It was shownthat the method was applicable for high cell density processesof bothE. coliandP. pastoris.

The combination of Bis- (1,3-dibutylbarbituric acid)trimethine oxonol (depolarised membranes) and propidium iodide(loss of membrane integrity) as fluorescent markers was usefulto measure viability at-line of cells in high cell densityprocesses. The protocol was shown to be reproducible forE. coliandP. pastoris.

The viability staining was used to study the kinetics ofweak organic acids (food preservatives). The protocol provideddata about cell functions such as membrane depolarisation andloss of membrane integrity caused by introducing weak organicacids to shake flask cultures ofE. coli.

Labeling inclusion bodies with fluorescent antibodiesprovided a method, which could specifically monitor theincreased accumulation of recombinant promegapoetin proteinwith process time. This technique was further developed forintracellular staining by application of a permeabilising stepbefore labeling with antibodies. Staining of inclusion bodiesdirectly inside permeabilised cells gave information about thedistribution of protein expression in the cell population.

In conclusion, flow cytometry provides an at-line, singlecell technique for measurement of several biological parametersin bioprocesses.

Key words: flow cytometry, Partec PAS, propidium iodide(PI), bis- (1,3-dibutylbarbituric acid) trimethine oxonol(BOX), Alexa fluor 488, bioprocess,E. coli,P. pastoris, inclusion body, food preservatives,viability, membrane potential

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Helou, Michael [Verfasser]. "Magnetic Flow Cytometry / Michael Helou." Kiel : Universitätsbibliothek Kiel, 2018. http://d-nb.info/1169132596/34.

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Stewart, Justin William. "Photonic Crystal-Based Flow Cytometry." Scholar Commons, 2014. https://scholarcommons.usf.edu/etd/5396.

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Photonic crystals serve as powerful building blocks for the development of lab-on-chip devices. Currently they are used for a wide range of miniaturized optical components such as extremely compact waveguides to refractive-index based optical sensors. Here we propose a new technique for analyzing and characterizing cells through the design of a micro-flow cytometer using photonic crystals. While lab scale flow cytometers have been critical to many developments in cellular biology they are not portable, difficult to use and relatively expensive. By making a miniature sensor capable of replicating the same functionality as the large scale units with photonic crystals, we hope to produce a device that can be easily integrated into a lab-on-chip and inexpensively mass produced for use outside of the lab. Using specialized FDTD software, the proposed technique has been studied, and multiple important flow cytometry functions have been established. As individual cells flow near the crystal surface, transmission of light through the photonic crystal is influenced accordingly. By analyzing the resulting changes in transmission, information such as cell counting and shape characterization have been demonstrated. Furthermore, correlations for simultaneously determining the size and refractive indices of cells has been shown by applying the statistical concepts of central moments.
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Sobek, Daniel. "Microfabricated fused silica flow chambers for flow cytometry." Thesis, Massachusetts Institute of Technology, 1997. http://hdl.handle.net/1721.1/10262.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1997.
Includes bibliographical references (leaves 107-116).
by Daniel Sobek.
Ph.D.
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Collins, Gary Stephen. "Multivariate analysis of flow cytometry data." Thesis, University of Exeter, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324749.

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Books on the topic "Flow cytometers"

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Macey, Marion G., ed. Flow Cytometry. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-451-3.

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Jacquemin-Sablon, Alain, ed. Flow Cytometry. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8.

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Ormerod, M. G. Flow cytometry. Oxford: BIOS Scientific Publishers in association with the Royal Microscopical Society, 1994.

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Ormerod, M. G. Flow cytometry. 2nd ed. Oxford, UK: Bios Scientific Publishers, 1999.

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Vielh, Philippe. Flow cytometry. New York: Igaku-Shoin Medical Publishers, 1991.

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Jaroszeski, Mark J., and Richard Heller. Flow Cytometry Protocols. New Jersey: Humana Press, 1997. http://dx.doi.org/10.1385/0896033546.

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Hawley, Teresa S., and Robert G. Hawley. Flow Cytometry Protocols. New Jersey: Humana Press, 2004. http://dx.doi.org/10.1385/1592597734.

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Shapiro, Howard M. Practical flow cytometry. 2nd ed. New York: A.R. Liss, 1988.

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Hawley, Teresa S., and Robert G. Hawley, eds. Flow Cytometry Protocols. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61737-950-5.

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Barteneva, Natasha S., and Ivan A. Vorobjev, eds. Imaging Flow Cytometry. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3302-0.

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Book chapters on the topic "Flow cytometers"

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Vorobjev, Ivan A., Aigul Kussanova, and Natasha S. Barteneva. "Development of Spectral Imaging Cytometry." In Methods in Molecular Biology, 3–22. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3020-4_1.

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AbstractSpectral flow cytometry is a new technology that enables measurements of fluorescent spectra and light scattering properties in diverse cellular populations with high precision. Modern instruments allow simultaneous determination of up to 40+ fluorescent dyes with heavily overlapping emission spectra, discrimination of autofluorescent signals in the stained specimens, and detailed analysis of diverse autofluorescence of different cells—from mammalian to chlorophyll-containing cells like cyanobacteria. In this paper, we review the history, compare modern conventional and spectral flow cytometers, and discuss several applications of spectral flow cytometry.
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Amblard, F. "Fluid Mechanical Properties of Flow Cytometers and Assessment Cell-Cell Adhesion Forces." In Flow Cytometry, 205–17. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8_13.

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Shapiro, Howard. "How Flow Cytometers Work — and Don’t Work." In In Living Color, 39–56. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57049-0_5.

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Telford, William. "How Flow Cytometers Work: An Introduction for Biomedical Scientists." In Biomedical Research, Medicine, and Disease, 17–29. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003220404-4.

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Jensen, Bruce D. "Cytosolic Events Related to Cell Activation: Potential Interaction between Signal and Energy Transduction Pathways in Smooth Muscle Cells." In Flow Cytometry, 3–16. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8_1.

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Kieda, Claudine, Nadine Bizouarne, Véronique Denis, and Michèle Mitterrand. "T Lymphocytes Recognition Molecules in Homing : A Flow Cytometry Study of Lectin-Glycoconjugates Interactions." In Flow Cytometry, 155–64. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8_10.

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Carayon, P. "Three- and Four-Color Immunofluorescence Analysis by Flow Cytometry." In Flow Cytometry, 165–87. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8_11.

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Eisert, Wolfgang G. "Energy Transfer." In Flow Cytometry, 189–203. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8_12.

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DeJong, M. O., H. Rozemuller, J. G. J. Bauman, and J. W. M. Visser. "Use of biotin-labeled growth factors for receptor studies." In Flow Cytometry, 219–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8_14.

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Brown, Spencer C. "Cytometrie Tout Terrain or Bush DNA Cytometry." In Flow Cytometry, 227–41. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84616-8_15.

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Conference papers on the topic "Flow cytometers"

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Sklar, Larry A. "Lasers in Flow Cytometry and Biotechnology." In Compact Blue-Green Lasers. Washington, D.C.: Optica Publishing Group, 1992. http://dx.doi.org/10.1364/cbgl.1992.tha2.

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Lasers are used by the biomedical community in many applications. These include flow cytometry, microscope imaging, solution spectroscopy, and very recently, optical manipulation of cells and subcellular particles. This paper reviews applications of lasers involving fluorescent probes. In most applications, these technologies require ~10 mW of laser power focused to cellular dimension (except for the solution measurements). I estimate that there are several thousand flow cytometers, several hundred laser imaging systems, and several hundred laser spectroscopy instruments distributed between the biomedical, physics and chemistry communities. Optical trapping as a commercial technology is emerging rapidly at the time this paper is being written using IR diode lasers to manipulate the cells.
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Mariella, Jr., Raymond P., Richard G. Langlois, and Don A. Masquelier. "Flow cytometers which incorporate light collection via a flow-stream waveguide." In BiOS '97, Part of Photonics West, edited by Alexander V. Priezzhev, Toshimitsu Asakura, and Robert C. Leif. SPIE, 1997. http://dx.doi.org/10.1117/12.273636.

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Leary, James F. "Design of portable ultraminiature flow cytometers for medical diagnostics." In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, edited by Daniel L. Farkas, Dan V. Nicolau, and Robert C. Leif. SPIE, 2018. http://dx.doi.org/10.1117/12.2286545.

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Burenkov, Ivan A., Javier Sabines-Chesterking, and Sergey V. Polyakov. "Quantum measurement enables single-biomarker detection in flow cytometers." In Quantum Sensing, Imaging, and Precision Metrology, edited by Selim M. Shahriar and Jacob Scheuer. SPIE, 2023. http://dx.doi.org/10.1117/12.2649396.

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Nguyen, Nam-Trung, and Chaolong Song. "A Micro Optofluidic System for Counting and Size Measurement of Particles." In ASME 2011 9th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2011. http://dx.doi.org/10.1115/icnmm2011-58211.

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This paper reports the integration of a biconvex micro optofluidic lens into a flow cytometer. The complex optofluidic and microfluidic channel networks are integrated on a single chip. Flow cytometers are widely applied to environmental monitoring, industrial testing and biochemical studies. Integrating a fow cytometer into microfluidic networks helps to miniaturize the system and make it portable for field use. The integration of optical components, such as lenses, further improves the compactness and thus has been intensively studied recently. However, the current designs suffer from severe light scattering due to the roughness of the solid-based lens interface. In this paper, we propose a micro optofluidic system using an optofuidic liquid lens to focus the light beam. Benefting from the smooth liquid-liquid lens interface and the refractive index matching liquid as cladding streams, a light beam can be well focused without scattering. The variations of the signal peak values are reduced owing to the small beam width at the beam waist. Compared to the macroscale systems and microscale systems with solid lenses, the device presents a more efficient and accurate performance on both counting and sizing of particles. The paper reports an analytical parametric study of the lens, followed by the experimental performance of the cytometer. The cytometer was able to detect and discriminate particles with different sizes.
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Zhangjian, Li, Li Peiyang, Xu Jie, Shao Weiwei, Wang Ce, and Cui Yaoyao. "2D acoustic focusing in a rectangular micro-channel of commercial flow cytometers." In 2017 IEEE International Ultrasonics Symposium (IUS). IEEE, 2017. http://dx.doi.org/10.1109/ultsym.2017.8092938.

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Li, Zhangjian, Peiyang Li, Jie Xu, Weiwei Shao, Ce Wang, and Yaoyao Cui. "2D acoustic focusing in a rectangular micro-channel of commercial flow cytometers." In 2017 IEEE International Ultrasonics Symposium (IUS). IEEE, 2017. http://dx.doi.org/10.1109/ultsym.2017.8092952.

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Chen, Wei P., and Ningyi D. Luo. "Component validation of direct diode 488nm lasers in BD Accuri C6 flow cytometers." In SPIE BiOS, edited by Ramesh Raghavachari, Rongguang Liang, and T. Joshua Pfefer. SPIE, 2016. http://dx.doi.org/10.1117/12.2213432.

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Wietzorrek, Joachim, E. Glossner, R. Kuehn, and V. Kachel. "Video imaging in flow - a novel method for increasing the discriminating power of flow cytometers used in aquatic science." In Europto Biomedical Optics '93, edited by Otto S. Wolfbeis. SPIE, 1994. http://dx.doi.org/10.1117/12.168746.

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Church, Christopher, Gaoyan Wang, Junjie Zhu, Tzuen-Rong Jeremy Tzeng, and Xiangchun Xuan. "Gentle Dielectrophoretic Focusing of Yeast Cells in Curved Microchannels." In ASME 2009 Second International Conference on Micro/Nanoscale Heat and Mass Transfer. ASMEDC, 2009. http://dx.doi.org/10.1115/mnhmt2009-18489.

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Focusing cells into a tight stream is usually a necessary step prior to counting, detecting and sorting them in, for example, microfluidic flow cytometers. We present herein a simple and gentle cell focusing technique in physiological solutions through a serpentine microchannel using DC-biased AC electric fields. This electrokinetic focusing eliminates sheath flows and in-channel microelectrodes. It results from the cross-stream dielectrophoretic motion of cells induced by the intrinsic channel curvatures. The effects of electric field magnitude, AC to DC electric field ratio, AC field frequency, and cell concentration on the focusing performance of yeast cells will be studied.
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Reports on the topic "Flow cytometers"

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Venkatesh, Mukund C. Optimization of the Mini-Flo flow cytometer. Office of Scientific and Technical Information (OSTI), June 1996. http://dx.doi.org/10.2172/388136.

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Heil, Cynthia A., Gabriel A. Vargo, David P. Fries, Ziaoling Ding, and David F. Millie. Flow Cytometer Based Biosensor for In-Field Cell Analysis. Fort Belvoir, VA: Defense Technical Information Center, September 2003. http://dx.doi.org/10.21236/ada630296.

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Cai, H., K. Kommander, P. S. White, and J. P. Nolan. Flow cytometry-based DNA hybridization and polymorphism analysis. Office of Scientific and Technical Information (OSTI), July 1998. http://dx.doi.org/10.2172/663513.

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CORSCADDENorscadden, Louise, and Arpaporn Sutipatanasomboon. The Definite Guide to Flow Cytometry for Scientists. ConductScience, December 2022. http://dx.doi.org/10.55157/cs20221213.

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Flow cytometry is an analytical technique that examines cells suspended in fluids. It uses a built-in laser beam to illuminate individual cells as the fluid passes through. The illumination causes fluorescence and scattered lights, which are emitted and reflected from the examining cell. These lights are split and filtered onto detectors and converted into electrical signals.
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Sklar, L. A., L. C. Seamer, F. Kuckuck, E. Prossnitz, B. Edwards, and G. Posner. Sample handling for kinetics and molecular assembly in flow cytometry. Office of Scientific and Technical Information (OSTI), July 1998. http://dx.doi.org/10.2172/663512.

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Chisholm, S. W., and B. J. Binder. Measurement of Synechococcus in situ growth rates using flow cytometry and rRNA-targeted probes. Final report. Office of Scientific and Technical Information (OSTI), February 1998. http://dx.doi.org/10.2172/355033.

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Marples, Brian, Olga Kovalchuk, Michele McGonagle, Alvaro Martinez, and Wilson, George, D. The Application of Flow Cytometry to Examine Damage Clearance in Stem Cells From Whole-Body Irradiated Mice. Office of Scientific and Technical Information (OSTI), February 2010. http://dx.doi.org/10.2172/972636.

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Jett, J. H. National flow cytometry and sorting research resource. Annual progress report, July, 1, 1994--June 30, 1995, Year 12. Office of Scientific and Technical Information (OSTI), April 1995. http://dx.doi.org/10.2172/475610.

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Loy, J. Dustin, and D. L. Hank Harris. Evaluation of in vivo Hemocyte Phagocytosis of Microsphere Beads in Litopenaeus vannamei Utilizing Flow Cytometry Following Administration of Bacterial Lipopolysaccharides. Ames (Iowa): Iowa State University, January 2010. http://dx.doi.org/10.31274/ans_air-180814-1257.

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Singh, Anjali. Ultimate Guide to Automated Cell Counter: Plus Purchasing Tips. ConductScience, June 2022. http://dx.doi.org/10.55157/cs20220614.

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An automated cell counter is a machine that uses either image analysis or electrical impedance principles to count cells automatically. The electrical impedance principle involves measuring changes in electrical resistance as cells pass through an aperture, while the light-scattering principle observes how cells scatter light when exposed to it. There are four main types of automated cell counting methods: Coulter Counter, Image Analysis Method, Flow Cytometry, and Stereological Cell Counting. Each method has its benefits and limitations, offering faster and more objective cell counting compared to manual methods, but also facing challenges like cost and potential counting inaccuracies. To use an automated cell counter, samples are prepared by pipetting cell suspension onto counting slide chambers, and the machine then provides a total cell count per ml.
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