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Gatrell, Jay D., Neil Reid, Thomas Steiger, Bruce W. Smith, and Michael C. Carroll. "“Value”-chains: Identity, tradition, and Ohio's flori(culture) industry." Applied Geography 29, no. 3 (July 2009): 346–57. http://dx.doi.org/10.1016/j.apgeog.2008.12.001.
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Sattar, Muhammad Muzamil, Asad Ali Qazi, Farhan Shahzad, and Abdul Rehman Shaikh. "Flori Pharma: maintaining ethics in an unethical sales environment." Emerald Emerging Markets Case Studies 10, no. 1 (March 13, 2020): 1–21. http://dx.doi.org/10.1108/eemcs-07-2019-0187.
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Learning outcomes The learning outcomes are as follows: what tasks are to be done by medical representatives in pharmaceutical industry? This study also highlights various competencies required to do effective selling in this industry; analyzes and discusses different unethical practices going on in the market; explains why ethical norms are necessary in sales context when sales targets are already achievable with unethical means; and develops and comments on strategies Flori Pharmaceutical can make to overcome on these unethical issues. What should be the response of Dahar to the email of Naveed khan? What course of action should be taken by Dahar in the deceitful reporting case of Mohsin Ali? Case overview/synopsis Flori is considered a leading and growing multinational organization in the highly competitive environment of Pakistan pharmaceutical industry with over 40 years of experience. The company aims to command a leading position in developing new health-care products as it offers a wide range of diabetic, cardiovascular, respiratory and vitamin products based on quality as a result of high research and examination. Recently, an email to Bilal Dahar on March 2017 from Flori’s star sales person Naveed Khan has forced management to take some strong decisions regarding ethical norms and values to be adopted by medical representatives of Flori pharmaceuticals. The email highlighted the issues related to sales pressure which are leading toward unethical sales practices. Dahar just not have to maintain Flori’s ethical code of conduct but he and his team also has to work hard to achieve more than 26% growth rate in sales revenue as compared to last year. Dahar knew that the highly competitive environment of pharmaceutical industry has led most of the stake holders to indulge in unethical behavior to achieve their individual targets. He knew that this is dangerous in long term for the multinational organizations such as Flori pharmaceuticals as if the similar behavior continues, the sales culture and values of the organization would be on stake. He also has to decide what decision to be taken against deceitful reporting issue of one of the top-performer territory managers, who was key person in helping Flori to close the sales year 2016 with the revenue of Rs. 6.4bn, a 26% growth over the last year. The case is rich enough to provide a platform regarding management of several ethical challenges in pharmaceutical selling and developing strategies based on them. Complexity academic level BBA, MBA final year. Supplementary materials Teaching Notes are available for educators only. Subject code CSS 8: Marketing.
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Zou, Wan Sheng, Yu Shuang Luo, Liang Guo Liu, Wen Bin Wang, and Pin Hong Yang. "Effect of a Freshwater Bacterium on Four Microcystis under Eutrophic Conditions." Applied Mechanics and Materials 295-298 (February 2013): 1636–42. http://dx.doi.org/10.4028/www.scientific.net/amm.295-298.1636.
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The effect of a freshwater bacterium Exiguobacterium sp.013 was examined on the cultures of four freshwater Microcystis which can cause cyanobacterial blooms. Four freshwater Microcystis cells and the bacterium were inoculated simultaneously, in modified BG-11 culture media, and cultured for 24 days continuously. The specific growth rate and cell densities were employed and determined in treated cultures and in controls.Additionally, dynamic changes of bacterium were investigated during the whole cultures. The results showed that the specific growth rate and maximal cell density of M. aeruginosa, M. flos-aquae and M. wesenbergii in treated cultures were significantly enhanced than that of control cultures, and daily cell densities of four Microcystis in the treated cultures were also significantly higher compared with that of control cultures. The added bacterial strain always kept dominance among the bacterial flora during the culture period. These findings implied that bacteria Exiguobacterium sp. might play a positive promoting role in forming Microcystis blooms under the eutrophication condition.
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Orak, Filiz, Hulusi Guven, Selma Ates, Adem Doganer, and Filiz Alkan Baylan. "‘’Evaluation of Flora Bacteria Grown in Blood Cultures: Are They Etiologic Agent of Infection or Only Contaminants?’’." Bangladesh Journal of Medical Science 20, no. 2 (February 1, 2021): 288–92. http://dx.doi.org/10.3329/bjms.v20i2.51537.
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Objective: In this study, it was aimed to determine whether the flora bacteria growing in blood cultures were infectious agents or only contaminants, for this purpose the hemogram parameters and other demographic characteristics of the patients were evaluated.
Materials and Methods: We evaluated 11.579 blood culture results using the BacT/ALERT® microbial detection system. The skin flora bacteria was detected in the blood cultures during one year and the rates of infectious agents among these bacteria were investigated retrospectively. The blood culture results, which were accepted as true bacteremia and contamination were compared in terms of positive blood culture flask count and inflammation markers (white blood cell count, neutrophil ratio, lymphocyte ratio and C-reactive protein levels).
Results: The total number of blood culture test was 11.579. Out of this number, 8205 (70.87%) was free of microbial growth and there was 3374 (29.13%) with microbial growth. 2609 (77.3%) of the positive cultures represented skin flora bacteria, 2510 (96.2%) of them were coagulase negative staphylococci. Only 50 (1.9%) of the flora bacteria were considered as infectious agents in terms of clinical and laboratory findings in addition to culture. A statistically significant correlation was found between true bacteremia and white blood cell (WBC) count and C-reactive protein (C-RP) levels (p <0.05).
Conclusion: Peripheral blood collection instead of catheter and using special phlebotomy teams should be taken into consideration in order to reduce contamination rates precautions such as adequate skin preparation, preparation of blood culture bottles and using single needle instead of double needle.
Bangladesh Journal of Medical Science Vol.20(2) 2021 p.288-292
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Cohen, Craig R., Lisa Gravelle, Samwel Symekher, Peter Waiyaki, Walter E. Stamm, and Julia A. Kiehlbauch. "Etiology of Persistent Tubo-Ovarian Abscess in Nairobi, Kenya." Infectious Diseases in Obstetrics and Gynecology 11, no. 1 (2003): 45–51. http://dx.doi.org/10.1155/s1064744903000061.
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ObjectiveTo study the microbial etiology of tubo-ovarian abscess (TOA).MethodsWe recruited 11 women in Nairobi, Kenya who failed antibiotic therapy alone and required surgical drainage of a presumptive TOA. Pus from the nine abscesses and two pyosalpinges were collected and cultured for aerobic, facultative and anaerobic microorganisms.ResultsEleven women suspected of having a TOA were hospitalized and treated for a median of 6 days (range 3–14 days) prior to surgical drainage of the abscess. Nine (82%) specimens were culture positive. Aerobes were present in all nine specimens. Seven of the nine positive cultures (78%) were polymicrobial and five of the polymicrobial cultures contained both anaerobes and aerobes. Anaerobic Gram-negative bacilli (Prevotella sp., Porphyromonas sp. and Bacteroides sp., Escherichia coli) and Streptococcus sp. (S. viridansandS. agalactiae) were the most common microorganisms isolated.Neisseria gonorrhoeaeandChlamydia trachomatiswere not isolated by culture or detected by polymerase chain reaction.ConclusionsIn Kenya, persistent TOAs are associated with endogenous flora similar to that normally found in the gastrointestinal tract.
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Brennan, Peter A., Peter Willy, Rajiv Anand, and Anthony F. Markus. "Colonization of the Cleft Nasal Floor by Anaerobic Oral Flora in Patients with Oronasal Fistulae." Cleft Palate-Craniofacial Journal 40, no. 4 (July 2003): 431–32. http://dx.doi.org/10.1597/1545-1569_2003_040_0431_cotcnf_2.0.co_2.
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Objectives Aerobic oral bacteria only rarely colonize the cleft nasal floor in patients with patent oronasal fistula. There are no studies that have investigated whether anaerobic oral flora colonize this site and whether attempting to culture them is useful for assessing the patency of oronasal fistulae in the clinic. Design A prospective study of 13 patients with cleft with patent unilateral oronasal fistulae. Microbiological culture swabs were taken from the oral cavity and both nasal floors, with the unaffected side being used as a control. Following aerobic and anaerobic culture, bacterial isolates were identified and compared. Main Outcome Measure A significant growth of anaerobic oral bacteria from the cleft nasal floor when compared with the unaffected side. Results Aerobic oral flora was cultured from the oral cavity in all 13 patients. A light growth of aerobic oral flora was found in the cleft nasal floor in two patients, and anaerobic oral flora was cultured from the cleft nasal floor in the same two patients. No statistical correlation was found between growth of anaerobic flora and the cleft nasal floor (p = .48). Conclusions Like aerobic oral flora, anaerobic oral bacteria would appear to only rarely colonize the cleft nasal floor in patients with oronasal fistulae. This additional investigation does not appear to be helpful in the assessment of oronasal fistulae in the clinic.
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Bjurström, Lena, and Catharina Linde-Forsberg. "Long-term study of aerobic bacteria of the genital tract in breeding bitches." American Journal of Veterinary Research 53, no. 5 (May 1, 1992): 665–69. http://dx.doi.org/10.2460/ajvr.1992.53.05.665.
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Summary The aerobic bacterial flora of the genital tract was characterized in 59 bitches in an 18-month study. The bitches represented 4 breeds and were from 3 kennels. Collection of vaginal swab specimens for bacterial culturing was performed every month, except during estrus when specimens were collected every week (n = 826). The capsule of the swab containing transport media was broken before specimen collection to moisten the tip, which helped to reduce the number of negative cultures. All bitches whelped at least once during the study and, thus, had known reproductive functions. Pregnancy rates, Utter sizes, and pup mortality were within normal limits. Pasteurella multocida, β-hemolytic streptococci group G, and Escherichia coli were the most common bacteria isolated. Although these species generally were isolated from mixed cultures, pure cultures were obtained from 18% of the specimens. There was a tendency for the various breeds to differ in their vaginal bacterial flora. The flora also varied during the reproductive cycle. Pasteurella multocida was isolated significantly more often during proestrus, estrus, metestrus, and pregnancy, than during anestrus and the postpartum period, and β-hemolytic streptococci were isolated significantly more often during proestrus than during estrus, pregnancy, or the postpartum period. Staphylococcus intermedius was almost exclusively found after parturition. Culture results were negative for only 5.2% of specimens cultured. On the basis of our findings, bacterial culturing of vaginal swab specimens from bitches without signs of genital disease is of little value.
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Tosun, Mustafa, Umut Devrim Binay, Sümeyye Akyüz, Hasan Ölmez, and Faruk Karakeçili. "COVID-19 Pandemisi Sürecinde Artan El Hijyeni Uyumunun Göğüs Hastalıkları Yoğun Bakım Ünitesindeki Kültür Sonuçlarına Etkisi." Flora the Journal of Infectious Diseases and Clinical Microbiology 26, no. 4 (December 20, 2021): 584–93. http://dx.doi.org/10.5578/flora.20219603.
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Syed, Sadia, David T. Liss, Chris O. Costas, and Janis M. Atkinson. "Diversion Principle Reduces Skin Flora Contamination Rates in a Community Hospital." Archives of Pathology & Laboratory Medicine 144, no. 2 (July 11, 2019): 215–20. http://dx.doi.org/10.5858/arpa.2018-0524-oa.
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Context.— Blood culture contamination is a common problem faced by medical centers and leads to significant cost. A possible method to reduce contamination is to discard the initial aliquot of blood, which contains skin and bacteria. Objective.— To determine whether the rate of contaminant blood cultures could be reduced by changing the order of draw to divert the first 7 mL to a gold- or green-top tube. Design.— A preintervention and postintervention study was conducted. During the 18-month intervention phase (September 2015–February 2017), all nurses in the emergency department and inpatient floor phlebotomists collected blood cultures by drawing the first 7 mL of blood into a gold- or green-top tube followed by drawing blood for blood culture bottles. The 18 months immediately preceding the study period (February 2014–July 2015) were used for comparison. Results.— There was an overall statistically significant decrease in contamination rate from 2.46% in the prediversion protocol group to 1.70% in the postdiversion protocol group (P < .001). Emergency department drawn cultures and inpatient cultures showed significant decrease in contamination rates between the preprotocol and postprotocol groups, 2.92% versus 1.95% (P < .001) for emergency department, and 1.82% versus 1.31% (P = .03) for inpatient. We noted less month-to-month variation during the study period compared with the preintervention period. Conclusions.— By using this simple diversion method, we were able to improve blood culture contamination rates for our emergency department and inpatients while incurring no added cost to the procedure.
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KIYMET, Elif, Elif BÖNCÜOĞLU, Şahika ŞAHİNKAYA, Ela CEM, Miray YILMAZ ÇELEBİ, Aybüke AKASLAN KARA, Yeliz ORUÇ, Nuri BAYRAM, and İlker DEVRİM. "Do Pediatric Residents Collect Blood Culture with the Appropriate Technique?" Flora the Journal of Infectious Diseases and Clinical Microbiology 29, no. 1 (March 22, 2024): 62–68. http://dx.doi.org/10.5578/flora.202401830.
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Benninger, Michael S., Peter C. Appelbaum, James C. Denneny, David J. Osguthorpe, and James A. Stankiewicz. "Maxillary Sinus Puncture and Culture in the Diagnosis of Acute Rhinosinusitis: The Case for Pursuing Alternative Culture Methods." Otolaryngology–Head and Neck Surgery 127, no. 1 (July 2002): 7–12. http://dx.doi.org/10.1067/mhn.2002.124847.
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OBJECTIVE: Traditional assessments of the microbial flora associated with acute bacterial rhinosinusitis have relied on maxillary sinus punctures (taps) and culture. These taps are now considered the gold standard for obtaining cultures and are used as the method of identifying bacterial pathogens in antimicrobial trials. Maxillary sinus taps are limited by discomfort to the patients and technical concerns. Because of these factors, the standard of performing taps has limited antibiotic trials and microbial surveillance. Alternatives to maxillary sinus taps have been explored. STUDY DESIGN: We conducted a retrospective, systematic review of the literature from 1950 to 2000 of articles comparing culture techniques in the nose and paranasal sinuses for acute bacterial rhinosinusitis. RESULTS: Nasal cultures have poor correlation to maxillary sinus cultures, whereas there is 60% to 85% concordance between endoscopically guided middle meatal cultures and maxillary sinus cultures. These studies, however, are all limited by small sample sizes and therefore are inadequate to make any concrete recommendations regarding the relative role of endoscopically guided middle meatal cultures as a formal method of pathogen identification in acute bacterial rhinosinusitis. CONCLUSION: A formal prospective study with sufficient sample size to assess the concordance between the microbial flora of the maxillary sinus punctures and middle meatal cultures in acute rhinosinusitis is recommended.
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Isıyel, Emel, and Sevda Soydan. "Comparison of Two Cleaning Methods Intaking Urine Culture Samples in Children." Flora the Journal of Infectious Diseases and Clinical Microbiology 24, no. 2 (June 15, 2019): 107–12. http://dx.doi.org/10.5578/flora.67606.
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Coppola, Alessandro, Vincenzo La Vaccara, Tommaso Farolfi, Michele Fiore, Chiara Cascone, Sara Ramella, Silvia Spoto, et al. "Different Biliary Microbial Flora Influence Type of Complications after Pancreaticoduodenectomy: A Single Center Retrospective Analysis." Journal of Clinical Medicine 10, no. 10 (May 18, 2021): 2180. http://dx.doi.org/10.3390/jcm10102180.
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Background: Bacterobilia is associated with postoperative morbidity after pancreaticoduodenectomy (PD), mostly due to infectious complications. The aim of this study was to investigate the prevalence of bacteria species isolated from intraoperative biliary cultures, and related complications after PD. Methods: An ANOVA test was used to assess the prevalence of isolated bacterial species and postoperative complications. The odds ratio was computed to evaluate the association between biliary cultures and each complication, Endoscopic Retrograde CholangioPancreatography (ERCP) and each complication, ERCP and biliary cultures, Delayed Gastric Emptying (DGE) and Postoperative Pancreatic Fistula (POPF). Results: Positive biliary cultures were found in 162/244 (66%) PDs. Different prevalences of polymicrobial biliary culture were detected in patients with postoperative complications. In SSIs, a significant prevalence of biliary culture positive for E. coli, Klebsiella pneumoniæ and Enterococcus fæcalis (p < 0.001) was detected. Prevalences of polymicrobial biliary cultures with Escherichia coli, Klebsiella pneumoniæ, Enterococcus fæcalis and Enterococcus fæcium were significantly associated with POPF (p < 0.001). Biliary culture positive for Escherichia coli, Enterococcus fæcalis and Enterococcus fæcium showed a higher prevalence of intra-abdominal collection and DGE (p < 0.001). Notably, Escherichia coli was significantly associated with DGE as a unique complication (OR = 2.94 (1.30–6.70); p < 0.01). Conclusions: Specific prevalences of polymicrobial bacterobilia are associated with major complications, while monomicrobial Escherichia coli bacterobilia is associated with DGE as a unique complication after PD.
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Dongowski, Gerhard, Angelika Lorenz, and Horst Anger. "Degradation of Pectins with Different Degrees of Esterification by Bacteroides thetaiotaomicron Isolated from Human Gut Flora." Applied and Environmental Microbiology 66, no. 4 (April 1, 2000): 1321–27. http://dx.doi.org/10.1128/aem.66.4.1321-1327.2000.
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ABSTRACT A complete human fecal flora and cultures of defined species obtained from fecal flora were investigated in vitro to determine their ability to ferment the dietary fiber pectin. Bacteroides thetaiotaomicron was tested as a pectin-degrading microorganism alone and in coculture with Escherichia coli. Macromolecular pectins with different degrees of esterification were used as substrates in microbial degradation studies. The levels of oligogalacturonic acids formed in batch cultures were estimated during a 24- or 48-h incubation period by using high-performance thin-layer chromatography and high-performance anion-exchange chromatography. The spectrum and the amount of unsaturated oligogalacturonic acids formed as intermediate products of pectin fermentation changed permanently in the culture media during incubation with the complete fecal flora. After 24 h, no oligogalacturonic acids were detected. The pectin-degrading activities of pure cultures of B. thetaiotaomicron were lower than the pectin-degrading activity of a complete fecal flora. Cocultures of B. thetaiotaomicronand E. coli exhibited intermediate levels of degradation activity. In pure cultures of E. coli no pectin-degrading activity was found. Additionally, the rate of pectin degradation was affected by the degree of esterification of the substrate. Saturated oligogalacturonic acids were not found during pectin fermentation. The disappearance of oligogalacturonic acids in the later stages of fermentation with both the complete fecal flora and B. thetaiotaomicron was accompanied by increased formation of short-chain fatty acids.
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Knight, Graham, and Jennifer Smith. "High-Tech Feudalism: Warrior Culture and Science Fiction TV." Florilegium 15, no. 1 (January 1998): 267–82. http://dx.doi.org/10.3138/flor.15.014.
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"Richard III with aliens" is how Cornell (102) describes "Sins of the Father," an episode of Star Trek: The Next Generation (hereafter TNG) in which the Klingon warrior Worf, son of Mogh, seeks to restore his family's honour by exposing and challenging those responsible for falsely accusing his dead father of treason to the Klingon Empire. Worf is only pardy successful in his quest, and he remains a perpetually marginal figure whose identity is divided by his Klingon heritage, his childhood as a Klingon orphan raised by humans, and his current status as the only Klingon in Starfleet, the military arm of the Federation of Planets, an alliance of Earth and other worlds whose relationship with the Klingon Empire is marked by tension, suspicion and, at times, open hostility. As a result of these divisions and struggles, Worf's family is eventually stripped of its wealth and rank on the Klingon home-world, and Worf's brother Kurn seeks a ritual death as the only way to absolve his own and his family's disgrace.
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Su, Erin, and Rosemary She. "1312. Evaluation of a Multiplexed PCR Pneumonia Panel in a Tertiary Care Medical Center." Open Forum Infectious Diseases 8, Supplement_1 (November 1, 2021): S744. http://dx.doi.org/10.1093/ofid/ofab466.1504.
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Abstract Background Syndromic PCR testing for lower respiratory pathogens may give rapid, actionable results to aid in management decisions for suspected pneumonia cases. We sought to evaluate the performance of a multiplexed PCR pneumonia panel compared to routine microbiologic work-up in a tertiary care patient population. Methods Sputum and bronchoalveolar lavage (BAL) samples from Keck Medical Center (Los Angeles, CA) inpatients submitted for clinical microbiology work-up Dec 2019-Jun 2020 were tested by a multiplexed PCR panel (FilmArray Pneumonia Panel, BioFire Diagnostics). We compared panel results for typical bacterial pathogens to those of quantitative culture and susceptibility testing. We retrospectively determined the incidence of non-panel respiratory pathogens as detected by standard of care tests in this patient cohort. Results 68 of 180 samples yielded 80 positive bacterial PCR results: 34 were detected by both PCR panel and culture and 46 by PCR panel only, yielding a sensitivity of 100% (34/34) for pathogens detected and specificity of 73.1% (114/156) among negative cultures (normal flora or no growth). Concordant results had PCR Bin values ≥10^5 copies/mL whereas all 18 targets detected at 10^4 copies/mL were culture-negative. Among resistance gene targets, the panel detected 12 MRSA specimens, of which MRSA grew in only 4 cultures; E. coli and CTX-M in 1 specimen from which grew normal flora; and multiple gram-negative organisms and KPC in 1 specimen from which culture isolated carbapenem-resistant P. aeruginosa. Quantitation from positive BAL cultures (n=25) correlated weakly with PCR Bin values (R-squared=0.17). Non-PCR panel pathogens were detected in 22 of 180 (12.2%) specimens through routine methods (16 molds, 3 AFB, and 3 non-fermenter gram-negative bacteria). Conclusion The pneumonia panel had excellent sensitivity for its target bacterial pathogens, but results were often positive in negative cultures. This could be due to antecedent antibiotic therapy, differences in reporting threshold versus culture, or inability of PCR to discern results from normal flora. Non-panel pathogens were detected in a significant proportion in our population. The pneumonia panel should be implemented and interpreted carefully with consideration of antimicrobial stewardship. Disclosures All Authors: No reported disclosures
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Fast, Susan. "Cluster on the Middle Ages in Contemporary Popular Culture: Introduction." Florilegium 15, no. 1 (January 1998): 185–86. http://dx.doi.org/10.3138/flor.15.009.
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The five essays here published were first given at a conference entitled The Middle Ages in Contemporary Popular Culture, conceived by Madeleine Jeay of the McMaster Working Group on the Middle Ages and Renaissance, organised by that group and held at McMaster University in the spring of 1996. The twenty-eight papers given at the conference explored various themes concerning the ways in which the middle ages are represented in contemporary popular culture. Among these were studies of the renewed interest in witchcraft and alchemy; organisations such as the Society for Creative Anachronism and feast-extravaganza Medieval Times, which engage in historical reconstruction; the popular interest in Gregorian chant and other medieval musics; and the "use" of the middle ages in contemporary film and other media. The aim was to give voice to this phenomenon in our culture in a manner that was sympathetic, not judgmental—a manner that took the appropriation of aspects of the middle ages in our popular culture seriously and that tried to theorise this appropriation in order that we can better understand it.
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Özdemir, Burcu, İpek Mumcuoğlu, Esragül Akıncı, İlkem Acar Kaya, Ahmet Sertçelik, and Hürrem Bodur. "Anaerop İnfeksiyon Saptanan Vakaların Klinik Özellikleri, Etkenlerin Tür Düzeyinde Dağılımı ve Anaerop Bakteremilerde Fatalite." Flora the Journal of Infectious Diseases and Clinical Microbiology 25, no. 4 (December 30, 2020): 506–15. http://dx.doi.org/10.5578/flora.69469.
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Introduction: This study aimed to determine the distribution of anaerobic bacteria species, which are the causative agents of infection, in order to investigate the epidemiological and clinical characteristics of the cases and evaluate fatality in cases with an aerobic bacteremia. Materials and Methods: One hundred and twenty-five cases who were determined with 148 isolates of anaerobic bacteria were included into this study at Ankara Numune Training and Research Hospital Medical Microbiology Laboratory between January 2014-February 2018. The specimens requested to undergo anaerobic evaluation and transported to the laboratory under anaerobic suitable conditions were prepared with Gram staining and inoculated in the Schaedler agar. After appropriate incubation, the identification of isolated anaerobic bacteria at the level of species was performed by using matrix-assisted laser desorption time-of-flight (MALDI-TOF MS). Results: Of the 125 cases who had an anaerobic infection, 60% were males, and mean age was 52.69 ± 18.5 years. Intra-abdominal and skin and soft tissue infections were the most common source of infection. One hundred and twenty-eight clinical specimens (78 abscesses, 50 blood cultures) which were sent from cases were identified with 148 anaerobic bacteria species. We detected the most common isolated species as Bacteriodes spp., Actinomyces spp. and Clostridium spp., respectively. The most common anaerobic bacteria species isolated from blood cultures of 50 cases with anaerobic bacteremia were Bacteroides spp., Clostridium spp., and intra-abdominal infection was the most common source for anaerobic bacteremia. Fusobacterium nucleatum (5/6; 83.3%) was the most frequent species in Fusobacterium bacteremia. F. nucleatum bacteremia was detected in an older population and was often associated with underlying malignancy. F. necrophorum bacteremia occurred in a younger population without underlying comorbidities and was diagnosed as Lemierre’s syndrome. Fatality rate was 51% in anaerobic bacteremia. Fatality was significantly higher in patients who were followed up in the intensive care unit and had high Charlson index (p< 0.05). Conclusion: MALDI-TOF MS has become an important method for the identification of anaerobic bacteria with its reliable results in a very short time. The most common anaerobic microorganism was Bacteroides spp., and the two most frequent sources of infection were intraabdominal and skin and soft tissue infections. We observed that most of the cases with F. nucleatum bacteremia had malignancy and an older population. The case with F. necrophorum bacteremia was young and had no underlying comorbidities. In this study, fatality rate (51%) was detected high in anaerobic bacteremia. Therefore, the routine use of anaerobic blood culture bottles is important for the identification and for the effective treatment of cases with anaerobic bacteremia.
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Karvar, Şinasi, and Nuran Deliağaoğlu. "Evaluation of Species Distribution and Antifungal Susceptibility of Candida Species Isolated from Blood Culture." Flora the Journal of Infectious Diseases and Clinical Microbiology 28, no. 3 (September 22, 2023): 331–40. http://dx.doi.org/10.5578/flora.20239703.
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Romanov, Alexey S., Egor E. Olesov, Viktor N. Tsarev, Valentina N. Olesova, and Elena V. Glazkova. "Effect of the oil-ether complex of Siberian fir on the fungal and periodontopathogenic flora of the mouth (microbiological study)." Russian Journal of Dentistry 26, no. 2 (September 4, 2022): 89–94. http://dx.doi.org/10.17816/1728-2802-2022-26-2-89-94.
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BACKGROUND: Peri-implantitis is mainly caused by the activity of periodontal pathogens with insufficient oral hygiene, which causes the disintegration of intraosseous dental implants against the background of chronic inflammation in peri-implant tissues. The correct techniques in the post-prosthetic stage in patients with dental implants include medical examination with regular professional oral hygiene, which is complicated by low patient compliance. In this regard, there are high requirements for the level of individual oral hygiene and for the effectiveness of local hygiene products, among which dental rinses occupy an important place. As rinses, it is possible to use pine-containing substances, particularly a complex of oil-essential fir Solagift of a Siberian company (Tomsk).
AIM: To conduct a microbiological study of the sensitivity of periodontopathogens and Candida albicans to the oil-essential complex of Siberian fir of different concentrations.
MATERIAL AND METHODS: A number of periodontopathogens and C. albicans were cultured in the presence of a complex of oilessential Siberian fir in the following proportions: 1:5, 1:10, and 1:15. Incubation for up to 3 days was conducted in the Reverse Spinner RTS-1 bioreactor (BioSan, Latvia) with automatic analysis of the optical density of the culture (OD) at a wavelength of =850 nm. The optical density was measured in McFarland units (Mcf). The assessment of culture growth control was based on the analysis of the growth phases of periodontopathogens: adaptive (lag phase), exponential (log phase), stationary, and dying. The following clinical isolates of microorganisms were used: Streptococcus constellatus, Staphylococcus aureus, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and C. albicans.
RESULTS: Joint cultivation of periodontopathogens with the oilether complex of Siberian fir reduces the OD of cultures of clinical isolates when breeding coniferous substance 1:151:5 by 13.727.1% (A. actinomycetemcomitans), 18.362.0% (F. nucleatum), 30.056.4% (S. aureus), and 19.274.1% (S. constellatus). The studied coniferous complex suppresses the culture of C. albicans at a concentration of 1:5, reducing the OD of the culture of fungi in the microbiological experiment by 29.8%.
CONCLUSION. Joint cultivation of periodontopathogens with the oilether complex of Siberian fir reduces the OD of cultures of clinical isolates when breeding coniferous substance 1:151:5. The studied coniferous complex suppresses the culture of C. albicans at a concentration of 1:5, reducing the OD of the culture of fungi in the microbiological experiment by 29.8%. The bacteriostatic effectiveness of the Siberian fir oil-ether complex significantly exceeds the effect of the herbal preparation Stomatophyte.
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Tevaearai, Fanny, Maike Katja Sachs, Samia El-Hadad, Ladina Vonzun, Ueli Moehrlen, Luca Mazzone, Martin Meuli, Franziska Krähenmann, and Nicole Ochsenbein-Kölble. "Stage 2: The Vaginal Flora in Women Undergoing Fetal Spina Bifida Repair and Its Potential Association with Preterm Rupture of Membranes and Preterm Birth." Journal of Clinical Medicine 11, no. 23 (November 28, 2022): 7038. http://dx.doi.org/10.3390/jcm11237038.
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Introduction: Vaginal dysbiosis affects pregnancy outcomes, however, the relevance of abnormal findings on pre/post-surgical vaginal culture in women undergoing fetal spina bifida (fSB) repair is unknown. Objectives: To describe the incidence of normal and abnormal pre- and post-surgical vaginal microorganisms in fSB patients and to investigate potential associations between the type of vaginal flora and the occurrence of preterm prelabour rupture of membranes (PPROM) and preterm birth (PTB). Methods: 99 women undergoing fSB repair were eligible (2010–2019). Pre-surgical vaginal culture was routinely taken before surgery. Post-surgical cultures were taken on indication. Vaginal flora was categorized into four categories: healthy vaginal flora (HVF), bacterial vaginosis (BV), desquamative inflammatory vaginitis (DIV), and yeast infection. Results: The incidence of HVF, BV, DIV, or yeast infections was not statistically different between the pre- and postoperative patients. Furthermore, an abnormal pre/post-surgical vaginal flora was not associated with PPROM (OR 1.57 (0.74–3.32), p = 0.213)/OR 1.26 (0.62–2.55), p = 0.515), or with PTB (OR 1.19 (0.82–1.73), p = 0.315)/(OR 0.86 (0.60–1.24), p = 0.425). Conclusions: Abnormal vaginal microbiome was not associated with PPROM and PTB when appropriate treatment was performed.
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Duangjan, Kritsana, Watsamon Nakkhunthod, and Chayakorn Pumas. "Photoautotrophic Production of Hydrogen in Carteria Crucifera AARL G045 Co-Cultured with Bacterial Flora." Botanica 25, no. 2 (December 1, 2019): 145–55. http://dx.doi.org/10.2478/botlit-2019-0016.
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AbstractThis study reports on high potential of photoautotrophic biohydrogen production in co-culture of the novel hydrogen producing microalga Carteria crucifera AARL G045 with its bacterial flora. Hydrogen production from immobilised and free cells were compared under photoautotrophic and photofermentation conditions. Two bacterial floras of Carteria crucifera AARL G045 cultures were isolated and identified by 16S rDNA sequence analysis as Agrobacterium tumefaciens G045-01 and Aeromicrobium kwangyangensis G045-02. As a result, co-culture of Carteria crucifera AARL G045 and A. kwangyangensis G045-02 generated higher level of hydrogen gas than that of the mono algal culture by about 1.32 times. In addition, co-culture of both free cells of the microalga and the bacterium produced higher hydrogen than both co-immobilisation in calcium alginate by about 2.04 times.
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Karpuz, Tuncer, Hatice Yazısız, Özlem Koyuncu Özyurt, Özgül Çetinkaya, Betil Özhak, Gözde Öngüt, Dilek Çolak, and Dilara Öğünç. "Evaluation of Positivity Times of Isolates Growing in Blood Cultures." Flora the Journal of Infectious Diseases and Clinical Microbiology 27, no. 2 (June 29, 2022): 218–26. http://dx.doi.org/10.5578/flora.20229805.
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Fidan, Ebru, Gamze Alçı, and Ayşegül Karahasan. "Is it Possible to Reduce Contamination Rates in Urine Cultures?" Flora the Journal of Infectious Diseases and Clinical Microbiology 28, no. 2 (June 1, 2023): 253–63. http://dx.doi.org/10.5578/flora.20239924.
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Şahin, Elif Ayça, and Ayşe Kalkancı. "Growth Time of Candida Species in Blood Culture Bottles and Approaches to Improve This Time." Flora the Journal of Infectious Diseases and Clinical Microbiology 27, no. 2 (June 29, 2022): 241–48. http://dx.doi.org/10.5578/flora.20229807.
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Alıcı, Ayşe, Gülgün Yenişehirli, and Aydan Yenişehirli. "Investigation of Azole Susceptibility Profile and Virulence Factors of Candida Strains Isolated From Blood Culture." Flora the Journal of Infectious Diseases and Clinical Microbiology 28, no. 3 (September 22, 2023): 411–20. http://dx.doi.org/10.5578/flora.20239711.
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Delany, Sheila. "English 380: Literature in Translation: Medieval Jewish Literature; Studies in medieval culture." Florilegium 20, no. 1 (January 2003): 201–5. http://dx.doi.org/10.3138/flor.20.047.
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Jewish culture has a continuous existence of nearly three millennia. This course isolates a small portion of it to read, in translation, work composed during the Middle Ages by authors from several countries and in several genres: parable and fantasy, lyric and lament, polemic, marriage manual, romance. Some of our material has not been translated into English before and is not yet available in print. We are fortunate to have brand-new pre-print copies of Meir of Norwich and especially of the famous Yiddish romance the Bovo-buch (in the course-pack)—an early modern version of a widely-read (non-Jewish) medieval text. Primary texts will be supplemented by scholarly books on which each student will offer a short class presentation.
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DALDABAN DİNÇER, Şölen, Özgür YANILMAZ, Ülkü ORAL ZEYTİNLİ, Ertan ÖZYURT, Ramazan AYAŞ, and Sebahat AKSARAY. "Comparison of Fluorescence Flow Cytometry and Culture Method for Identification of Urine Specimens with No Growth." Flora the Journal of Infectious Diseases and Clinical Microbiology 24, no. 4 (December 2019): 321–28. http://dx.doi.org/10.5578/flora.68138.
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Cimiotti, Jeannie P., Fann Wu, Phyllis Della-Latta, Mirjana Nesin, and Elaine Larson. "Emergence of Resistant Staphylococci on the Hands of New Graduate Nurses." Infection Control & Hospital Epidemiology 25, no. 5 (May 2004): 431–35. http://dx.doi.org/10.1086/502418.
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AbstractObjective:To describe the aerobic microbial flora on the hands of experienced and new graduate nurses over time.Design:A prospective cohort design that examined the relationship between duration of employment in an intensive care unit (ICU) and the microbial flora on the hands of experienced and new graduate nurses during a 23-month period.Setting:A 50-bed, level III-IV neonatal ICU in New York City.Participants:Twelve experienced nurses and 9 new graduate nurses working full time in the NICU.Intervention:One hundred fifty samples were obtained from the clean, dominant hands of the nurses. Cultures were performed at baseline and then quarterly for each experienced and new graduate nurse. Baseline and final cultures ofStaphylococcus epidermidiswere further examined using pulsed-field gel electrophoresis.Results:At baseline, a significantly larger proportion of the experienced nurses had methicillin-resistant, coagulase-negative staphylococci isolated from their hands compared with the new graduate nurses (95% and 33%, respectively;P= .0004). For a second culture, performed 1 to 4 months later, there were no longer significant differences between the two groups (82% and 54%, respectively;P= .12). By the last culture, all staphylococcal isolates were methicillin resistant in both groups of nurses; 3 were methicillin-resistantS. aureus.Conclusions:Colonization with methicillin-resistant staphylococci occurred after brief exposure to the hospital environment, despite the use of antiseptic hand hygiene agents. Furthermore, at final culture, the two groups shared one dominant hospital-acquired strain ofS. epidermidis.
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Khan, Muhammad N., Raghavan Vidya, and Richard E. Lee. "The Limited Role of Microbiological Culture and Sensitivity in the Management of Superficial Soft Tissue Abscesses." Scientific World JOURNAL 6 (2006): 1118–23. http://dx.doi.org/10.1100/tsw.2006.215.
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The aim of this study was to assess the role of the routine practice of microbial culture and sensitivity at incision and drainage of superficial soft tissue abscesses. The case notes of 162 consecutive patients, selected from the microbiology database over a period of 1 year, were reviewed. All had incision and drainage of superficial soft tissue abscesses and included perianal, pilonidal, axillary, and breast abscesses. Patients with chronic wounds, recurrent abscesses, diabetes, pregnancy, and immunosuppression were excluded. The impact of pus culture and sensitivity (C/S) on management and clinical outcome was documented. Out of 162 patients, 97 were male (59.8%) and 65 were female (40.1%). Only 115 (70.9%) yielded positive cultures and 47 (29.1%) were sterile. The cultured microbial flora was predictable and sensitive to empirical antibiotics. In four patients, the results of microbial culture sensitivity showed microbial resistance to empirical antibiotics; however, it did not affect the management or the outcome for these patients. The routine practice of sending swabs for C/S after incision and drainage of superficial soft tissue abscesses does not contribute significantly towards patient management. Most patients are already on antibiotics prior to the referral and in the remainder, surgeons start antibiotics empirically. These broad-spectrum antibiotics cover the common pathogens involved, and there is no significant change in the antibiotic treatment after reviewing the culture reports following incision and drainage of uncomplicated superficial skin abscesses.
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Karimsab, D., and SK Razak. "Study of aerobic bacterial conjunctival flora in patients with diabetes mellitus." Nepalese Journal of Ophthalmology 5, no. 1 (March 25, 2013): 28–32. http://dx.doi.org/10.3126/nepjoph.v5i1.7818.
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Introduction: Normal bacterial flora may be altered by a variety of factors. Objective: To study the aerobic bacterial conjunctival flora in patients with diabetes mellitus and to find its clinical significance by comparing the results to the conjunctival flora of non-diabetic subjects. Materials and methods: A total of 75 diabetic patients were included as cases and 25 nondiabetics as controls to compare the results. Specimens for the study of conjunctival flora were taken by rubbing sterile cotton-tipped swabs to the inferior palbebral conjunctiva. The conjunctival culture report of the patients with diabetic mellitus was compared to that of nondiabetic subjects. Results: Positive conjunctival cultures were seen in a higher percentage of patients with diabetes (unilateral and bilateral positive conjunctival cultures 34.66 % and 58.66 % respectively) compared to that in non-diabetic controls (unilateral and bilateral positive conjunctival cultures 24 % and 16 % respectively). Diabetics showed a higher proportion of coagulase negative staphylococci (45.33 %), compared to the non-diabetic group (16 %). Among the diabetic patients, positive conjunctival cultures were detected more frequently in those with diabetic retinopathy compared to those without retinopathy. A higher proportions of bilateral positive conjunctival cultures were seen in cases with proliferative diabetic retinopathy (38.63 %) in comparison to patients with no retinopathy and different stages of non-proliferative diabetic retinopathy. Conclusion: The conjunctival floral pattern with increased bacteria in diabetics is a predominant cause of many diabetes-related ocular infections. The presence of diabetic retinopathy is an indicator for increased colonization of conjunctiva, and its severity correlates with the severity of diabetic retinopathy. Nepal J Ophthalmol 2013; 5(9):28-32 DOI: http://dx.doi.org/10.3126/nepjoph.v5i1.7818
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ÇALIK, Şebnem, Selma TOSUN, Ümmügülsüm ALTIN, Alpay ARI, Ali Ilgın OLUT, and Özlem Yüksel ERGİN. "Is There Clinical Significance to Coagulase-Negative Staphylococci Isolated from Blood Cultures?" Flora the Journal of Infectious Diseases and Clinical Microbiology 22, no. 1 (March 1, 2017): 34–41. http://dx.doi.org/10.5578/flora.58643.
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Groeneveld, Leanne. "Salvation, Damnation, and the Wounded (Corporate) Body of Christ in Late Medieval Culture." Florilegium 22, no. 1 (January 2005): 81–104. http://dx.doi.org/10.3138/flor.22.004.
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Taşkın Kafa, Ayşe Hümeyra, Cem Çelik, Mürşit Hasbek, and Mustafa Zahir Bakıcı. "Pseudomonas aeruginosa: Evaluation of Antimicrobial Resistance Patterns in Strains Isolated from Blood Culture in a University Hospital." Flora the Journal of Infectious Diseases and Clinical Microbiology 25, no. 3 (September 15, 2020): 308–15. http://dx.doi.org/10.5578/flora.68734.
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Introduction: The aim of this study was to contribute to the establishment of appropriate empirical treatment protocols by determining antibiotic susceptibility profiles of Pseudomonas aeruginosa strains isolated from bacteremia patients in intensive care units and various clinics. Materials and Methods: Antibiotic susceptibility of a total of 111 P. aeruginosa isolates isolated from the blood of inpatients in intensive care units and other services between January 2014 and July 2018 in Cumhuriyet University, Faculty of Medicine were evaluated retrospectively. In the study, the susceptibility profiles of amikacin, piperacillin-tazobactam, ceftazidime, cefepim, gentamicin, ciprofloxacin, levofloxacin, imipenem and meropenem were investigated using the automated system of Phoenix 100 (Becton Dickinson Co., Sparks, MD, USA). The results were evaluated based on the criteria of the Clinical and Laboratory Standards Institute (CLSI) for 2014 and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) from 2015 onwards. Results: One hundred and eleven P. aeruginosa were isolated from blood samples, 77 from intensive care units and 34 from various services of the hospital. P. aeruginosa strains isolated from cultures have a high rate of resistance against antimicrobial agents. The highest resistance was determined for imipenem with 20.7% and the lowest resistance was determined for amikacin with 1.8%. Piperacillintazobactam, ceftazidim, cefepim, ciprofloxacin, levofloxacin, and meropenem resistance rates were 18%, 17.1%, 17.8%, 18.9%, 13.9%, 19.8% respectively. Multidrug-resistant was found to be 10.8%. This ratio was determined as 13% in intensive care units. Conclusion: Considering the current antibiotic susceptibility profiles while determining empirical treatment protocols is rather important to prevent resistance. In addition, putting forward the bacterial resistance status periodically through this type of studies, to determine the strength of existing drugs and to guide therapy, presents valuable data. Within this context, we propose that the data of our study will contribute to the current literature
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Maksimovic, A., M. Nurkic, Z. Maksimovic, and M. Rifatbegovic. "Vaginal bacteria of healthy cats during different stages of their oestrous cycle." Journal of the Hellenic Veterinary Medical Society 74, no. 2 (July 9, 2023): 5737–842. http://dx.doi.org/10.12681/jhvms.30266.
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Little is known about vaginal and uterine bacteria in clinically healthy cats and their correlation with different stages of the oestrus cycle. The differences in vaginal bacterial flora between household and stray queens remains unknown. The aim of this study was to investigate the occurrence of vaginal and uterine bacteria in clinically healthy household and stray queens and to correlate culture findings with specific stages of the oestrous cycle. Vaginal and uterine samples from 40 clinically healthy queens were collected for isolation of bacteria and cytological examination. Bacteria were isolated from 31 vaginal swabs (77.5%) from stray (16/20; 80%) and household (15/20; 75%) cats. The isolates were more frequently detected in pure culture (18/31; 58%) than in mixed cultures (13/31; 41.9%). Streptococcus spp. was the most commonly identified bacteria (n = 16; 51.6%), followed by coagulase negative Staphylococcus spp. (n = 15; 48.4%) and E. coli (n = 12; 38.7%). A mixed bacterial culture of E. coli and Streptococcus spp. was commonly detected (50%), mainly in households (66.7%), whereas a mixed culture of Staphylococcus spp. and Streptococcus spp. (41.6%) was commonly isolated from stray cats (60%). The frequency of isolation of pure or mixed bacterial cultures and the isolates did not vary significantly during the different stages of the oestrus cycle. All uterine samples tested were negative for bacteria. This study identified the most common bacteria in the vagina of clinically healthy cats. The isolation of vaginal bacteria in pure or mixed cultures should be considered as normal finding. The stage of the oestrous cycle apparently does not affect vaginal bacterial flora. Vaginal bacteria may differ between stray and households cats. No bacteria can be isolated from the uterus of clinically healthy cats.
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Waugh, Robin. "Wilfrid Laurier University." Florilegium 20, no. 1 (January 2003): 83–84. http://dx.doi.org/10.3138/flor.20.024.
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Smaller universities can preside over profound achievements in disciplines such as medieval studies through the fostering of commitment and focus. For instance, the profile of medieval studies has developed significantly in the last three years at Wilfrid Laurier University, where, in December of 2002, an interdisciplinary program in medieval studies was approved by the university’s senate. Prior to the development of this program, medieval material was taught largely within the traditional disciplines. The new program is designed to include course subjects from a variety of national and religious traditions. It will have four core courses: History 101, Medieval Europe 500-1100; History 102, The High Middle Ages; Medieval Studies 100, Discovering the Middle Ages (Knights, Saints, and Dragons); and Medieval Studies 200, The Medieval World View. The last two courses are designed to be team-taught by faculty members from various disciplines, such as Classics, History, Religion and Culture, Music, Fine Arts, Languages and Literatures (particularly French and Spanish), and English and Film. Participating faculty members have contributed many of their existing courses and research interests to the program, together with many new ideas for lectures, fourth-year seminars, and innovations in teaching. The program will also include courses in medievalism, that is, the study of representations of medieval cultural materials within contemporary cultures and sensibilities. Though these kinds of offerings, such as a “Tolkien and Fantasy” course, are growing at Laurier and are currently more popular among students than the more traditional medieval-themed courses, the former variety of course is not pushing aside the latter. In fact, an interdisciplinary medieval studies program should help the existing medieval courses at Laurier appeal to more students.
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Ka, Modou, Mame Samba Mbaye, Ndongo Diouf, Samba Laha Ka, Mamadou Faye, and Kandioura Noba. "Flore adventice du niébé (Vigna unguiculata [L] Walp.) dans la zone nord du bassin arachidier (Niakhène-Sénégal)." International Journal of Biological and Chemical Sciences 17, no. 2 (May 29, 2023): 349–62. http://dx.doi.org/10.4314/ijbcs.v17i2.5.
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Le niébé (Vigna unguiculata [L.] Walp.) est l’une des principales cultures du bassin arachidier sénégalais. Il joue un rôle important dans les programmes de sécurité alimentaire et dans les stratégies de développement socio-économique du pays. Toutefois, plusieurs contraintes (maladies, ravageurs, pression des adventices, etc.) entraînent des baisses de rendements de cette spéculation. Il est donc important, de trouver des solutions adéquates à ces contraintes ce qui passerait par la connaissance des agents responsables. C’est pourquoi cette étude était entreprise pour contribuer à l’amélioration de la production du niébé par la caractérisation de la flore adventice du niébé. Pour ce faire des inventaires ont été effectués dans les parcelles de niébé dans la zone de Niakhène en appliquant la méthode du « tour de champ ». Ceci a permis de recenser 92 espèces réparties dans 67 genres et 29 familles. Cette flore est dominée par les dicotylédones avec 76,09% des espèces recensées contre 23,93% de monocotylédones. Les familles dominantes sont les Fabaceae et les Poaceae avec respectivement 21,74% et 16,30% des espèces. Les thérophytes (77,17%) sont largement dominantes suivies des phanérophytes (16,30%). Les géophytes, les chaméphytes et les parasites regroupent respectivement 3,26%), 2,17% et 1,09% de la flore. Au plan biogéographique, les espèces Africaines sont plus importantes avec 41,30% de la flore suivies des espèces Pantropicales avec 28,26%.
Cowpea (Vigna unguiculata [L.] Walp.) is one of the main crops of the Senegalese groundnut basin. It plays an important role in the country’s food security programs and socio-economic development strategies. However, several constraints (diseases, pests, weed pressure, etc.) lead to yield reductions in this crop. It is therefore important to find adequate solutions to these constraints, which would require knowledge of the agents responsible. This study was undertaken to contribute to the improvement of cowpea production by characterizing the cowpea weed flora. Inventories were carried out in the cowpea plots in the Niakhène area using the « field tour » method. This made it possible to identify 92 species distributed in 67 gendera and 29 families. This flora was dominated by dicotyledons with 76.09% of species recorded against 23.93% of monocotyledons. The dominant families were Fabaceae and Poaceae with 21.74% and 16.30% of species respectively. The therophytes (77.17%) were largely dominant followed by phanerophytes (16.30%). The geophytes, the chamaephytes and parasites group represented 3.26%, 2.17% and 1.09% of the flora, respectively. At the biogeographical level, African species are more important with 41.30% of the flora, followed by Pantropical species with 28.26%.
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ZİYADE, Nihan, Neval ELGÖRMÜŞ, Çiğdem ARABACI, Erdoğan KARA, and Ferah KARAYEL. "Evaluation of Salmonella Species Isolated in Post-mortem Bacteriological Cultures in Autopsy Cases." Flora the Journal of Infectious Diseases and Clinical Microbiology 24, no. 4 (December 2019): 313–20. http://dx.doi.org/10.5578/flora.68295.
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Eryıldız, Canan, Kıymet Tabakçıoğlu, Sezgin Kehaya, Nermin Şakru, and Şaban Gürcan. "Lysinibacillus massiliensis Isolated from the Synovial Fluid: A Case Report." Flora the Journal of Infectious Diseases and Clinical Microbiology 25, no. 4 (December 30, 2020): 595–98. http://dx.doi.org/10.5578/flora.70014.
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Lysinibacillus massiliensis is an aerobic, endospore-forming, gram-negative staining bacterium with peritrichous flagella belonging to the Bacillaceae family. A few cases of L. massiliensis isolated from the cerebrospinal fluid and tissue have been reported. In this study, we aimed to describe a case of L. massiliensis isolated from the synovial fluid. The synovial fluid from a 74-year-old female patient was inoculated into blood culture bottle. Gram-negative rods were observed in a gram-stained smear from a positive blood culture bottle. The bacterium was identified as Lysinibacillus sphaericus/Lysinibacillus fusiformis, with a probability of 89% using an automated bacterial identification system (VITEK2; Biomerieux, France). Subsequently, 16S rRNA gene sequencing was performed, and the sequence was analyzed using the Basic Local Alignment Search Tool. The sequence had 99.9% (1426/1427) identity with the strain L. massiliensis (GenBank ID: NR_043092.1). To our knowledge, this is the first reported case of L. massiliensis isolated from the synovial fluid. When an endospore-forming gram-negative staining bacterium can not be identified by phenotypic characterization, L. massiliensis should be considered, and different microbiological methods should be used for identification.
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Romanov, A. S., V. N. Tsarev, V. N. Olesova, and I. S. Makhneva. "Degree of Suppression of Fungal Flora of the Mouth When Using Oil-Ether Complex from Fir Needle." A.I. Burnasyan Federal Medical Biophysical Center Clinical Bulletin, no. 2 (July 2022): 60–63. http://dx.doi.org/10.33266/2782-6430-2022-2-60-63.
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The main cause of peri-implantitis is the activity of periodontal pathogens with insufficient oral hygiene, which causes disintegration of intraosseous dental implants against the background of chronic inflammation in peri-implant tissues. The correct tactics of the post-prosthetic stage in patients with dental implants is medical examination with regular professional oral hygiene, which is complicated by low patient compliance. In this regard, there are high requirements for the level of individual oral hygiene and for the effectiveness of local hygiene products, among which dental rinses occupy an important place. As rinses, it is possible to use pine-containing substances, in particular, a complex of oil-essential fir of Siberian production of the company “Solagift” (Tomsk). The purpose of the study: microbiological study of the sensitivity of periodontopathogens and C. albicans fungi to the oil-essential complex of Siberian fir of different concentrations. Material and methods: a number of periodontopathogens and C. albicans were cultured in the presence of a complex of oil-essential Siberian fir in the following proportions 1:5, 1:10, 1:15. Incubation lasting up to three days was carried out in the Reverse Spinner RTS-1 bioreactor (BioSan, Latvia) with automatic analysis of the optical density of the culture (OD) at a wavelength of λ = 850 nm. The optical density was measured in McFarland units (Mcf). The assessment of culture growth control was based on the analysis of the growth phases of periodontopathogens: adaptive (lag phase), exponential (log phase), stationary, dying. The following clinical isolates of microorganisms were used: Streptococcus constellatus; Staphylococcus aureus; Fusobacterium nucleatum; Aggregatibacter actinomycetemcomitans, as well as Candida albicans. Results of the study: Joint cultivation of periodontopathogens with the oil-ether complex of Siberian fir reduces the optical density of cultures of clinical isolates when breeding coniferous substance 1:15 – 1:5 by 13.7% – 27.1% (A. actinomycetemcomitans), 18.3% – 62.0% (F. nucleatum), 30.0% – 56.4% (S. aureus), 19.2% – 74.1% (S. constellatus). The studied coniferous complex suppresses the culture of C. albicans at a concentration of 1:5, reducing the optical density of the culture of fungi in the microbiological experiment by 29.8%
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Zhao, Chunpu, Jilin Xu, Shanliang Xu, Gege Bao, and Danli Wang. "Study on the Microflora Structure in a Litopenaeus vannamei–Sinonovacula constricta Tandem-Culture Model Based on High-Throughput Sequencing under Different Culture Densities." Fishes 8, no. 6 (May 26, 2023): 287. http://dx.doi.org/10.3390/fishes8060287.
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In this study, we evaluated the intestinal contents of Pacific whiteleg shrimp (Litopenaeus vannamei), the visceral mass of razor clams (Sinonovacula constricta) and the water columns and the substrate sediments in different culture-density groups in a L. vannamei–S. constricta tandem-culture model by high-throughput sequencing of the 16S rRNA gene. The results show that the culture density affected the bacterial floral structure of the water columns, substrate sediment and razor-clam gut masses without making significant differences in the bacterial flora structure of the shrimp gut; the Shannon diversity indexes of the bacterial communities in the substrate sediment, shrimp gut and razor-clam gut masses were not significantly different among the density groups, and the Shannon diversity index of the bacterial communities in the water column was higher in the group with higher culture densities; at the phylum level, the dominant bacteria common to the shrimp guts, razor-clam visceral mass, water columns and substrate sediment were Proteobacteria and Bacteroidetes; Chloroflexi was the dominant bacterium specific to the substrate sediment; and Firmicutes was the dominant bacterium specific to the shrimp gut and razor-clam gut mass. We used national standards (GB 17378.4-2007, China) to evaluate the content of water-quality factors through the environmental factors and the genus-level correlation analysis of bacterial flora that follow: the dominant bacterium in the water column, uncultured_bacterium_f_Rhodobacteraceae, was negatively correlated with PO43−-P; the dominant bacteria in the substrate sediments, uncultured_bacterium_f_Anaerolineaceae and Woeseia, were significantly and negatively correlated with DO; and the dominant bacteria Lactococcus spp. in the razor-clam gut mass and the shrimp intestines were positively correlated with DO. These results show that culture density directly affects water-quality factors, which in turn affect the culture environment and the composition structure of the bacterial flora in a cultured organism.
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Chandra, Subhash, Jagpal Singh Klair, Kaartik Soota, Daniel J. Livorsi, and Frederick C. Johlin. "Endoscopic Retrograde Cholangio-Pancreatography-Obtained Bile Culture Can Guide Antibiotic Therapy in Acute Cholangitis." Digestive Diseases 37, no. 2 (October 3, 2018): 155–60. http://dx.doi.org/10.1159/000493579.
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Background: Only a small proportion of patients with biliary tree infection grow microorganisms in blood cultures. Antibiotics chosen or tailored based on organisms identified on blood cultures have a potential for under-treatment and unfavorable outcomes, including recurrent infection and early stent occlusion. In our current practice, we collect bile for culture if an Endoscopic Retrograde Cholangio-Pancreatography (ERCP) is performed in patients with suspected cholangitis. In this study, we compare the microbial yield of blood cultures and ERCP-obtained bile cultures in patients with ascending cholangitis. Methods: We reviewed medical records of all the patients treated for ascending cholangitis who had blood cultures and ERCP-obtained bile cultures at a tertiary care center between 2010 and 2016. Bile was collected for culture before injecting contrast, via a catheter after discarding the initial 3 mL. Results: Ninety-three patients were included with mean age of 71 (±15) years. Out of 93 patients, 11 (12%) had prior sphincterotomy, 29 (31%) had an indwelling biliary stent, and malignant obstruction was the most common etiology (34%). ERCP-obtained bile cultures were positive in 90 out of 93 (97%) patients with monomicrobial growth in 34 out of 93 (39%) patients. Mixed intestinal flora was noted in 3 patients. Blood cultures were positive in only 30 out of 93 patients (32%) and 24 out of 93 (26%) patients had monomicrobial growth. Totally 26 out of 30 patients (87%) grew the same organism as the bile culture, 3 grew an organism different from bile cultures, and one had no growth in the bile culture. On multivariable analysis, the presence of an indwelling biliary stent was the lone factor associated with polymicrobial growth, 83 vs. 52%, p = 0.007. Conclusion: ERCP-obtained bile cultures are a reliable and feasible mechanism to evaluate patients with suspected biliary tree infection. This technique has a significantly higher yield when compared to blood culture. Selection and tailoring of antibiotics based on bile culture in the management of ascending cholangitis are advised.
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Vemu, Lakshmi, Sukanya Sudhaharan, Neeraja Mamidi, and Padmasri Chavali. "Need for appropriate specimen for microbiology diagnosis of chronic osteomyelitis." Journal of Laboratory Physicians 10, no. 01 (January 2018): 021–25. http://dx.doi.org/10.4103/jlp.jlp_14_17.
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Abstract INTRODUCTION: Chronic osteomyelitis (COM) is a common infection, especially in developing countries. An adequate bone biopsy specimen processed with appropriate microbiology culture methods for isolation and identification of the causative organisms is considered as the gold standard for the diagnosis of osteomyelitis. MATERIALS AND METHODS: The present study is a retrospective microbiology analysis of the specimen from 219 clinically diagnosed cases of COM between January 2013 and April 2016. RESULTS: The overall culture positivity was 111/219 (50. 6%), colonization was seen in 22/219 (10.5%), while the rest 86/219 (39.3%) were culture-negative specimen; culture positivity was highest from tissue specimen (71/113, 62.8%). Among the swabs, 40/106 (37.7%) were culture positive. About 28/40 (70%) culture-positive swabs showed significant growth of Gram-positive organisms. Colonization with skin flora such as diphtheroids and Coagulase-negative Staphylococci was seen in 22/106 (20.7%) of the swabs. Sterile cultures (44/106, 41.6%) were high among the swab specimen. Gram-positives were most common (75/111, 67.56%). Staphylococcus aureus was the predominant organism isolated in 70/111 (63%) cases. Gram-negative bacilli showed a high level of antibiotic resistance. CONCLUSION: As per our data, the culture yield from wound swabs was low or contaminated with normal skin flora, as compared to the biopsy or tissue specimen. Hence, an appropriate sampling of the infected bone using recommended protocols is highly essential for improving microbiological yield and the outcome of COM.
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Lack, H. Walter, and Giovan Battista Ferrari. "Flora overo cultura di fiori." Taxon 51, no. 3 (August 2002): 597. http://dx.doi.org/10.2307/1554891.
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Vargas Pacheco, Cristina, and Naïl Muniglia. "“Viajar hacia lo diverso con Paul Gauguin y Flora Tristán”: una experiencia de mediación cultural y circulación artística en el norte peruano (2018-2019)." Illapa Mana Tukukuq, no. 17 (December 25, 2020): 162–75. http://dx.doi.org/10.31381/illapa.v0i17.3480.
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En 2018, el Ministerio de Cultura del Perú lanzó una convocatoria nacional bajo el nombre de Estímulos Económicos de la Cultura, buscando motivar la generación de propuestas en diferentes ámbitos de las artes plásticas, musicales y escénicas. Uno de los proyectos que se vio favorecido fue “Viajar hacia lo diverso con Paul Gauguin y Flora Tristán: proyecto para la dinamización, circulación y mediación de la creación artística en la región norte”, impulsado desde la Alianza Francesa de Piura. En el presente artículo se narra esta experiencia, que tuvo como eje de concepción y acción, la mediación cultural y, particularmente, artística, partiendo del recuento teórico de su surgimiento en un contexto de democratización cultural. Palabras clave: mediación cultural, Paul Gauguin, Flora Tristán, Alianza Francesa de Piura, norte peruano, estímulos económicos de la cultura.
AbstractIn 2018 the Peruvian Ministry of Culture launched the Estímulos Económicos de la Cultura, an open national call intended to receive and support project ideas in the fields of music, scenic and visual arts. One of the beneficiaries of the grant has been the project “Travelling towards the diversity with Paul Gauguin and Flora Tristán: project to foster the dynamization, circulation, and mediation of artistic creation in the northern region of Peru”, promoted by the Alliance Française of Piura. The present article aims to explain the whole experience process whose core of conception and action is the cultural and artistic mediation, departing from the theoretical paradigm of its emergence in a context of cultural democratization.Keywords: cultural mediation, Paul Gauguin, Flora Tristán, North of Peru, state economic support, Alliance Française of Piura.
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Khubutiya, M. Sh, A. M. Gasanov, E. A. Tarabrin, T. V. Chernen’kaya, T. E. Kallagov, and E. I. Pervakova. "A comparison of airway microbiota in donors and recipients of lung transplants." Russian Pulmonology 29, no. 2 (July 1, 2019): 184–88. http://dx.doi.org/10.18093/0869-0189-2019-29-2-184-188.
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This study was aimed at investigation of relationships between bronchial washing culture in post-transplant recipient and bronchial flora of the lung donor. Methods. A comparative analysis of bronchial washing cultures from 30 post-transplant lung recipients was performed. All lung donors were stratified to ideal, suboptimal and marginal donors according to the lung transplant suitability. Results. As a result, development of post-transplant pulmonary complications was directly related to bacterial flora of the donor lung. The incidence of pneumonia in post-transplant patients was 3.3% after transplantation of ideal donor lungs, 20% after transplantation of suboptimal donors lungs and 100% after transplantation of marginal donor lungs. Conclusion. The rate of pneumonia in transplanted lungs was directly related to bronchial flora in the donor lungs. This should be taken into account when planning antibacterial therapy after lung transplantation.
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Rao, Kulkarni Manik, and Panchami . "A descriptive study to determine the bacterial flora and antibiotic sensitivity of lower respiratory tract in tracheostomised patients." International Journal of Otorhinolaryngology and Head and Neck Surgery 6, no. 4 (March 25, 2020): 701. http://dx.doi.org/10.18203/issn.2454-5929.ijohns20201286.
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<p class="abstract"><strong>Background:</strong> Tracheostomy is one of the commonest operations performed by an otorhinolaryngologist for various indications and in different age groups and tracheo-bronchitis is a common complication in tracheostomised patients. This study designed to determine the bacterial flora and antibiotic sensitivity of lower respiratory tract following tracheostomy in hospitalized patients.</p><p class="abstract"><strong>Methods:</strong> This is a descriptive study of 50 tracheostomised patients carried out from December 2017 to May 2019, at VIMS Ballari. </p><p class="abstract"><strong>Results:</strong> Stridor was the most common indication for tracheostomy (70%). Tracheal aspirate was sent for bacterial culture and sensitivity on day 0, 1 week and 3 weeks post tracheostomy. Majority of the patients showed no growth in the day 0 and 3weeks post tracheostomy bacterial cultures of the tracheal aspirate. Bacterial cultures yielded growth in majority of the patients in the 1-week post tracheostomy cultures. The sensitivity pattern, intermediate response pattern and resistance pattern to the first line antibiotics that are regularly used in our hospital were studied in all the 3 samples of tracheal aspirates that were sent for bacterial culture from all the 50 tracheostomised patients.</p><p class="abstract"><strong>Conclusions:</strong> It is a good practice to send the tracheal aspirate for culture and sensitivity following Tracheostomy. If the patient is started on suitable antibiotics based on the culture postoperative recovery will be hastened, risk of postoperative infections like tracheitis, tracheobrochitis, stoma site infection and other lower respiratory tract infections can be reduced.</p>
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Watson, Jasmine, Alexandria Owens, Kavita Imrit-Thomas, Miranda Malone, Andy Tobias, and Lakshmi Goudar. "An Appeal to Incorporate Hand Hygiene Education into Standard Elementary School Curriculum." Open Forum Infectious Diseases 4, suppl_1 (2017): S411. http://dx.doi.org/10.1093/ofid/ofx163.1028.
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Abstract Background The Center for Disease Control and Prevention reports that 160 million school days are lost each year due to infectious illnesses. Hand hygiene is one of the most effective ways to prevent illness that can lead to absenteeism among school-aged children, yet few schools have a formal education program as a preventative strategy. Methods A Pilot Hand Hygiene experiment was initiated for 90 second grade students at a Virginia Beach Public School. The experiment was designed to bring awareness and to satisfy a scientific module requirement. Students cultured their hands on general purpose agar plates with the assistance of physicians and a microbiologist. The proper hand washing technique was demonstrated. Students were equally divided into two groups: hand washing group and sanitizer group. They were instructed to re-culture hands after intervention. Students observed cultures for five days and documented results. Results Overall, student observation of decreased microbial growth was an average of 91% (Figure 1). Education improved compliance, which resulted in a favorable behavioral change on average of 89% (Figure 2). There was a 71% decrease in incidence of illness-related absences 30 days after the hand hygiene intervention (Figure 3). In three out of five classes, hand sanitizer was more effective when compared with hand washing. In addition, a random sample of cultures were incubated in a microbiology lab to identify the common microbes among the second-grade elementary school population. It revealed both resident and transient flora. Post-intervention, there was a rise in coagulase-negative Staphylococci resident flora. This indicated a successful decrease in transient flora, which is most likely to cause illness. Conclusion Hand Hygiene education is remarkably beneficial, especially in children who are at greater risk of illness. It is clearly effective in decreasing infectious disease risk, while teaching a life-long skill. For the impact as a preventative strategy to be felt, its implementation into elementary school curriculum is warranted. Disclosures All authors: No reported disclosures.
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Sultan, Naomi, Irina Shchors, Marc V. Assous, and Maskit Bar-Meir. "The NICU flora: An effective technique to sample surfaces." PLOS ONE 16, no. 9 (September 23, 2021): e0257821. http://dx.doi.org/10.1371/journal.pone.0257821.
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Objective Environmental surface sampling in healthcare settings is not routinely recommended. There are several methods for environmental surface sampling, however the yield of these methods is not well defined. The aim of the present study is to compare two methods of environmental surface sampling, to characterize the neonatal intensive care unit (NICU) flora, compare it with rates of infection and colonization and correlate it with the workload. Design and setting First, the yield of the swab and the gauze-pad methods were compared. Then, longitudinal surveillance of environmental surface sampling was performed over 6 months,once weekly, from pre-specified locations in the NICU. Samples were streaked onto selective media and bacterial colonies were identified using matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF). Results The number of colonies isolated using the gauze pad method was significantly higher compared with the swab method. Overall, 87 bacterial species of 30 different bacterial genera were identified on the NICU environmental surfaces. Of these, 18% species were potential pathogens, and the other represent skin and environmental flora. In 20% of clinical cultures and in 60% of colonization cultures, the pathogen was isolated from the infant’s environment as well. The number of bacteria in environmental cultures was negatively correlated with nurse/patient ratio in the day prior to the culture. Conclusion The gauze pad method for environmental sampling is robust and readily available. The NICU flora is very diverse and is closely related with the infants’ flora, therefore it may serve as a reservoir for potential pathogens.
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Ramakrishnan, Ramya, Ramakrishnan V. Trichur, Sowmya Murugesan, and Srihari Cattamanchi. "Analysis of the microbial flora in breast abscess: a retrospective cohort study conducted in the emergency department." International Surgery Journal 4, no. 7 (June 22, 2017): 2143. http://dx.doi.org/10.18203/2349-2902.isj20172599.
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Background: Breast abscess develops as a complication of lactational mastitis. Recently, there is an increase in the incidence of non-lactational breast abscess. The aim of the study was to analyse the microbial flora in the lactational and non-lactational breast abscess in the ED and to elucidate the susceptibility of flora to different antibiotics.Methods: This is a retrospective cohort study of breast abscess patients registered in the ED over two years. The case records of these patients were retrieved, and the details of their age, clinical presentation, investigation, and treatment were recorded. Specifically, the pus culture sensitivity and antibiotics used in the management of these patients were noted, and the pattern of microbiological flora analyzed.Results: A total of 124 patients were included in the study, with 97 women were categorized into lactational breast abscess, and 27 women were classified as a non-lactational breast abscess. Mean age was 24 years of age (Range 18 to 56 years). The majority of the women were young between 18 and 34 years of age (83%). The culture was grown in 92% of the patient pus specimens. Staphylococcus aureus (83%) was common organisms cultured in lactational breast abscess. Mixed Flora including Staphylococcus aureus and Group B Streptococci were grown in non-lactational breast abscess.Conclusions: Appropriate antibiotic choices are of immense importance in the management of breast abscess. Mixed flora is common in non - lactational breast abscess when compared with a lactational breast abscess. Staphylococcus Aureus is the most common isolate in both groups.