Dissertations / Theses: 'Floral biology'

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au, Lynleys@calm wa gov, and Lynley M. Stone. "Floral Biology and Propagation of Blue-Flowered Conospermum Spp." Murdoch University, 2003. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20040824.145625.

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Blue-flowered Conospermum are endemic to Western Australia, and show great potential as cut flowers. Propagation from cuttings or seed proved difficult, and root initiation in vitro is problematic. This thesis examines the floral biology of the species and the possibility of using somatic embryogenesis to overcome propagation problems. A survey of explant tissue types for C. eatoniae and C. caeruleum was carried out to identify tissue that could be induced into embryogenic pathways. Vegetative, semi-floral and floral buds were initiated into culture from February to June, but were found unsuitable for embryogenesis, producing shoots, callus or dying in culture. Leaves from in vitro leaf cultures formed callus in the presence of 2,4-D and BAP, but were unable to differentiate into embryos in the presence of a variety of growth regulator combinations and concentrations. Immature zygotes died in culture. Direct embryogenesis and/or embryogenic callus was observed on mature zygotes of the species C. caeruleum, C. spectabile, C. dorrienii and C. brownii, and somatic embryos were maintained in culture for up to 18 months for C. caeruleum. Maturation and germination of somatic embryos proved difficult; treatments of cold, ABA, desiccation or mannitol did not induce maturation. It appears that developmental pathways in Conospermum are well defined and are difficult to alter in vitro. It was concluded that somatic embryogenesis has limited commercial potential in these species. Conospermum species have an active pollination mechanism where the style is held in a state of tension when the flower opens. When pressure is applied at the base of the style by an insect, the style flicks downwards, striking the insect pollinator and releasing pollen from the anther in a single dusty mass. However, the breeding systems of blue-flowered Conospermum have not previously been well explored. Flowers on a C. eatoniae inflorescence opened from the basal end upwards acropetally, with the terminal two or three buds never opening. Fruit and seed set occurred only from the basal one to three buds. Isolation of C. eatoniae and C. amoenum flowers showed they were unable to self-pollinate in the absence of insect pollinators. Experiments to determine the timing of the peak of stigmatic receptiveness were inconclusive. Pollen germinated and penetrated the stigma 0 ¡V 6 days after anther dehiscence. Pollen loads on the stigma did not relate to the number of pollen tubes observed down the style. Controlled pollinations of cultivated C. eatoniae at a field station using self and cross pollen, revealed compatibility with a range of pollen genotypes, as pollen tubes were observed extending down the style. However, late-acting incompatibility could not be ruled out as controlled crosses failed to set any seed as flowers were shed from the bush. DNA analysis of open pollinated C. eatoniae seed progeny from two plants from a field station and two plants in natural bushland revealed very different pollination habits. Plants from the field station showed no outcrossing, with progeny closely resembling the maternal parent, whereas plants from the wild population showed outcrossing with several different paternal parents. These results suggest self-pollinated seed can be reliably obtained in a plantation situation using stands of ramets of the same clone. Alternatively, assuming that the required insect pollinators are present in a cultivated stand, it should be possible to obtain cross pollinated seed by surrounding the maternal plant with the desired paternal parent. Unusual pollen behaviour was observed for many blue-flowered species, a white-flowered species of Conospermum, and close relative, Synaphea petiolaris. Up to three pollen tubes emerged from the triporate pollen in vitro, and at rates of up to 55 Ýms-1. This rate was maintained for only 2 s but is greater than 20 times faster than reported in the literature for any species, in vitro or in vivo. Pollen with multiple tubes was also observed on the stigma in vivo in C. amoenum flowers. Changing the osmotic pressure of the germination medium by altering sucrose concentration influenced the number of tubes to emerge from the pollen grain; generally the number of tubes decreased as sucrose increased. However, the rate of tube growth was unaffected. The addition of calcium channel blockers to the germination medium had no effect on Conospermum growth rate, nor did they eliminate pulses of tube growth. Observation of Conospermum pollen ultrastructure revealed similarities to Gramineae pollen. The tube cytoplasm was packed with vesicles filled with material of similar electron density to the cell wall. Few golgi were identified, and the apical end of the tube contained these vesicles, smaller secretory vesicles and mitochondria. This is atypical of the tip, which is normally free of large vesicles. Distinct zones in the cytoplasm were not identified, which is similar to Gramineae. Like the grasses, Conospermum appears to pre-manufacture cell wall material and store it in vesicles ready for rapid germination and extension. A biological function of multiple pollen tube emergence with such rapid growth was not elucidated. This research has shown Conospermum to be a complex and very interesting genus. Further investigation into the remarkable growth of multiple pollen tubes would enhance our knowledge of the biological processes involved in tube growth and the process of fast wall formation. The potential benefits to the cut flower industry of commercialising some of these species warrants further effort to find an efficient method of propagation. Introduction into horticulture may be the only means by which these threatened species will survive.

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Stone, Lynley M. "Floral biology and propagation of blue-flowered Conospermum spp." Stone, Lynley M. (2003) Floral biology and propagation of blue-flowered Conospermum spp. PhD thesis, Murdoch University, 2003. http://researchrepository.murdoch.edu.au/351/.

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Blue-flowered Conospermum are endemic to Western Australia, and show great potential as cut flowers. Propagation from cuttings or seed proved difficult, and root initiation in vitro is problematic. This thesis examines the floral biology of the species and the possibility of using somatic embryogenesis to overcome propagation problems. A survey of explant tissue types for C. eatoniae and C. caeruleum was carried out to identify tissue that could be induced into embryogenic pathways. Vegetative, semi-floral and floral buds were initiated into culture from February to June, but were found unsuitable for embryogenesis, producing shoots, callus or dying in culture. Leaves from in vitro leaf cultures formed callus in the presence of 2,4-D and BAP, but were unable to differentiate into embryos in the presence of a variety of growth regulator combinations and concentrations. Immature zygotes died in culture. Direct embryogenesis and/or embryogenic callus was observed on mature zygotes of the species C. caeruleum, C. spectabile, C. dorrienii and C. brownii, and somatic embryos were maintained in culture for up to 18 months for C. caeruleum. Maturation and germination of somatic embryos proved difficult; treatments of cold, ABA, desiccation or mannitol did not induce maturation. It appears that developmental pathways in Conospermum are well defined and are difficult to alter in vitro. It was concluded that somatic embryogenesis has limited commercial potential in these species. Conospermum species have an active pollination mechanism where the style is held in a state of tension when the flower opens. When pressure is applied at the base of the style by an insect, the style flicks downwards, striking the insect pollinator and releasing pollen from the anther in a single dusty mass. However, the breeding systems of blue-flowered Conospermum have not previously been well explored. Flowers on a C. eatoniae inflorescence opened from the basal end upwards acropetally, with the terminal two or three buds never opening. Fruit and seed set occurred only from the basal one to three buds. Isolation of C. eatoniae and C. amoenum flowers showed they were unable to self-pollinate in the absence of insect pollinators. Experiments to determine the timing of the peak of stigmatic receptiveness were inconclusive. Pollen germinated and penetrated the stigma 0 - 6 days after anther dehiscence. Pollen loads on the stigma did not relate to the number of pollen tubes observed down the style. Controlled pollinations of cultivated C. eatoniae at a field station using self and cross pollen, revealed compatibility with a range of pollen genotypes, as pollen tubes were observed extending down the style. However, late-acting incompatibility could not be ruled out as controlled crosses failed to set any seed as flowers were shed from the bush. DNA analysis of open pollinated C. eatoniae seed progeny from two plants from a field station and two plants in natural bushland revealed very different pollination habits. Plants from the field station showed no outcrossing, with progeny closely resembling the maternal parent, whereas plants from the wild population showed outcrossing with several different paternal parents. These results suggest self-pollinated seed can be reliably obtained in a plantation situation using stands of ramets of the same clone. Alternatively, assuming that the required insect pollinators are present in a cultivated stand, it should be possible to obtain cross pollinated seed by surrounding the maternal plant with the desired paternal parent. Unusual pollen behaviour was observed for many blue-flowered species, a white-flowered species of Conospermum, and close relative, Synaphea petiolaris. Up to three pollen tubes emerged from the triporate pollen in vitro, and at rates of up to 55 mcgms-1. This rate was maintained for only 2 s but is greater than 20 times faster than reported in the literature for any species, in vitro or in vivo. Pollen with multiple tubes was also observed on the stigma in vivo in C. amoenum flowers. Changing the osmotic pressure of the germination medium by altering sucrose concentration influenced the number of tubes to emerge from the pollen grain; generally the number of tubes decreased as sucrose increased. However, the rate of tube growth was unaffected. The addition of calcium channel blockers to the germination medium had no effect on Conospermum growth rate, nor did they eliminate pulses of tube growth. Observation of Conospermum pollen ultrastructure revealed similarities to Gramineae pollen. The tube cytoplasm was packed with vesicles filled with material of similar electron density to the cell wall. Few golgi were identified, and the apical end of the tube contained these vesicles, smaller secretory vesicles and mitochondria. This is atypical of the tip, which is normally free of large vesicles. Distinct zones in the cytoplasm were not identified, which is similar to Gramineae. Like the grasses, Conospermum appears to pre-manufacture cell wall material and store it in vesicles ready for rapid germination and extension. A biological function of multiple pollen tube emergence with such rapid growth was not elucidated. This research has shown Conospermum to be a complex and very interesting genus. Further investigation into the remarkable growth of multiple pollen tubes would enhance our knowledge of the biological processes involved in tube growth and the process of fast wall formation. The potential benefits to the cut flower industry of commercialising some of these species warrants further effort to find an efficient method of propagation. Introduction into horticulture may be the only means by which these threatened species will survive.

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Buchmann, Stephen L. "Floral Biology of Jojoba (Simmondsia chinensis), an Anemophilous Plant." University of Arizona (Tucson, AZ), 1987. http://hdl.handle.net/10150/554232.

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Simmondsia chinensis is a widespread perennial plant native to the Sonoran Desert of the United States and northern Mexico. Individuals are dioecious with small unisexual flowers borne on separate plants. The plants are strictly wind-pollinated (anemophilous). Honey bees (Apis mellifera) and native bees often collect large amounts of pollen from male plants but are never found visiting female plants, as there are no floral attractants or rewards in the form of volatiles or nectar, in the green apetalous female flowers. Male plants produce copious amounts of pollen, up to an estimated 523 g/plant, [0.5-2.4 mg/flower, or 8.3-48.9 mg/inflorescence]. Per anther there are from 11,000 to 18,000 pollen grains. The pollen is small, smooth with little exine sculpturing and averages 34μ in equatorial diameter. There is almost no surface oily pollenkitt on the grains. Anthers dehisce and pollen is shed during the entire day, but with an early afternoon peak from 1300 to 1500 MST. This corresponds to peak atmospheric concentrations of 60-63 grains/cubic meter during this period. Seasonal data for Jojoba aerial pollen concentrations and selected hourly values for certain days are also presented for 1982 and 1983 in a native stand. Data on floral number, floral ontogeny, stigmatic receptivity, and seeds per fruit, are also presented for Jojoba.

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Venturieri, Giorgini Augusto. "Floral biology of cupuassu (Theobroma grandiflorum (Willdenow ex Sprengel) Schumann)." Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386982.

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Cupuassu (Theobroma grandiflorum), one of the most profitable crops of Amazonia, is now attracting world-wide attention as an exotic fruit, used in juices, ice cream and sweets. It is a shade tolerant tree that can be grown as a component of agroforestry systems. Nevertheless it is still a wild species and little is known about its biology. Floral biology of cupuassu was studied in Belem-Brazil during 2 floweringfruiting seasons between June 1991 and December 1993. Flowering occurs in the drier period of the year. Flowers commence opening at any time of the day, but open fully at the end of afternoon. The anthers dehisce and the stigmas are receptive as soon as the flowers are fully open. Stigmas remain receptive until 10:00 am the following day. Throughout this period, the pollen grains remain viable. The flowers have a complex morphology which favours allogamy. The species is also self-incompatible. Experimental pollinations, using compatible pollen grains, have shown that a flower which receives 60 compatible pollen grains has 20% probability of setting fruit; a flower which receives more than 400 pollen grains always sets fruit. However, only around 2% of naturally pollinated flowers receive more than 60 pollen grains. A stingless bee, Plebeia minima, is considered an effective pollinator of cupuassu. Another stingless bee, Trigonisca pediculana, also visits cupuassu flowers. Both bees are small insects, which are unlikely to fly very far. Ants (Wasmannia sp.) and weevils (Baris sp.) were considered secondary pollinators, unlikely to promote effective pollinationsFruits mature during the wet period of the year, approximately 5.5 months after the flowers open. The limited and irregular fruit set is probably caused by scarcity of pollinators. The transformation of cupuassu to a plantation crop will therefore require conditions which favour natural pollinators and their access to receptive and compatible flowers

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Fonseca, Marina de Magalhães da. "Biologia reprodutiva de Butia odorata (Barb. Rodr.) Noblick." Universidade Federal de Pelotas, 2014. http://repositorio.ufpel.edu.br:8080/handle/prefix/3076.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
Os estudos de biologia reprodutiva são fundamentais para a conservação e manejo das espécies de plantas, uma vez que o período reprodutivo é uma fase de grande importância para a dinâmica das populações e sobrevivência das espécies . Esta dissertação teve como objetivo contribuir para o conhecimento da morfologia floral, biologia floral e reprodutiva de Butia odorata (Barb. Rodr.) Noblick, além de identificar os agentes polinizadores e suas relações com as flores. O trabalho foi realizado em dois países, no Uruguai (em La Paloma, Departamento de Rocha) e no Brasil (em Porto Alegre, Rio Grande do Sul). Foram identificadas as fases de floração, antese masculina e feminina. Foi estimado o número de flores masculinas e femininas por ráquila na inflorescência, e a relação existente entre flores masculinas e femininas. Foi verificada a coloração das ráquilas e das flores. As flores foram caracterizadas quanto à sua morfologia e morfometria. Foram identificadas as ordens de insetos que visitaram as inflorescências, a relação dos insetos com as flores, os recursos florais coleta dos e o período de visitação durante as fases de floração. As flores de Butia odorata são unissexuais, hipóginas, actinomorfas, diclamídeas e sésseis. As flores masculinas e femininas ocorrem na mesma inflorescência e possuem formatos distintos, sendo as primeiras muito mais numerosas do que as segundas. As flores masculinas estão dispostas por toda a ráquila, com maior concentração do meio para o ápice, as flores femininas são encontradas do meio para a base da ráquila, formando tríades com duas masculinas. Existe variabilidade genética para cor e tamanho de ráquilas e para cor das flores em inflorescências de B. odorata. A espécie apresenta mecanismos de protandria. Uma grande diversidade de espécies de insetos das ordens Hymenoptera, Coleoptera e Diptera visitam as inflorescências. Durante a antese masculina, as ordens Hymenoptera e Coleoptera são mais frequentes, enquanto que durante a antese feminina a ordem Hymenoptera se destaca. Os insetos são atraídos pelos recursos como pólen e néctar disponibilizados pelas flores. A compreensão sobre a biologia reprodutiva, apresentado nos dois capítulos da dissertação, serve de alicerce para a preservação do butiá, pois este conhecimento é de grande importância para traçar estratégias de conservação e uso de recursos genéticos e programas de melhoramento.
Studies of reproductive biology are essential for the conservation and management of plant species, once the reproductive period is a time of great importance for the population dynamics and species survival. This dissertation had as objective to contribute to uderstanding floral morphology, floral and reproductive biology of Butia odorata (Barb. Rodr.) Noblick. Also, to identify the pollinators and their relationship with the flowers. The study was conducted in two Countries, Uruguay (La Paloma, Rocha Department) and Brazil (Porto Alegre, Rio Grande do Sul State). Stages of flowering, and male and female anthesis were identified. The number of male and female flowers per rachille in the inflorescence, and the ratio between male and female flowers were estimated. The coloring of rachilles and flowers was verified. The morphology and morphometry of flowers were characterized. Orders of insects that visited the inflorescences, the relationship of insects with flowers, the collected floral resources and the period of visitation during the stages of flowering were identified. Flowers of Butia odorata are unisexual, hypogynous, actinomorphic, dichlamydeous and sessile. The male and female flowers occur on the same inflorescence and have different formats, being the male more numerous than the female. The male flowers are arranged throughout the rachille, with higher concentration from the middle to the apex. The female flowers are found from the middle to the base of rachille, forming triads with two male flowers. There is genetic variability for color and size of rachilles and for color of flowers on inflorescences of B. odorata. The species has mechanisms of protandry. A great diversity of insects' species, belonging to the Hymenoptera, Coleoptera and Diptera orders visit the inflorescences. During male anthesis, the Hymenoptera and Coleoptera orders are more frequent, whereas during female anthesis the Hymenoptera order stands. The insects are attracted by the resources such as pollen and nectar provided by the flowers. The understanding of the reproductive biology, presented in two chapters of this dissertation, serves as the foundation for the preservation of butiá, because this knowledge is of great importance to devise strategies for the conservation and use of genetic resources and breeding programs.

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Bomfim, Isac Gabriel Abrahão. "Uso de abelhas sem ferrão (Meliponinae:Apidae) em casa de vegetação para polinização e produção de frutos com e sem semente de minimelancia [Citrullus lanatus (Thunb.) Matsum. &Nakai]." reponame:Repositório Institucional da UFC, 2013. http://www.repositorio.ufc.br/handle/riufc/17026.

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BOMFIM, Isac Gabriel Abrahão. Uso de abelhas sem ferrão (Meliponinae:Apidae) em casa de vegetação para polinização e produção de frutos com e sem semente de minimelancia [Citrullus lanatus (Thunb.) Matsum. &Nakai]. 2013. 140 f. Tese (doutorado em zootecnia)- Universidade Federal do Ceará, Fortaleza-CE, 2013.
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The aim of this thesis was to investigate the viability of using the stingless bees, Melipona subnitida and Scaptotrigona sp. nov. for pollination and fruit production of seeded and seedless mini watermelon (Citrullus lanatus) under greenhouse conditions. To this end, the floral biology and pollination requirements of seeded and seedless mini watermelon varieties were investigated, as well as the adaptive and foraging behavior of both meliponines. The experiment was carried out in a greenhouse situated in the city of Fortaleza-CE. The results revealed that the varieties studied were monoecious plants with diclinous flowers, and that the stigma of its pistillate flowers remained receptive throughout the anthesis, which was from 05:25 h to 14:20 h. The seeded varieties set fruits by geitonogamous and xenogamous pollination, within the same variety and between different diploid genotypes. In contrast, the seedless genotypes set fruits only by cross-pollination with pollen from seeded varieties. M. subnitida did not show any interest in the crop, under the experimental conditions. On the other hand, Scaptotrigona sp. nov. adapted well to the confinement and collected floral resources since the second day after its introduction. Scaptotrigona sp. nov. showed an essential behavior for the pollination of mini watermelon, as they visited, for direct collection of nectar, staminate and pistillate flowers of both diploid and triploid genotypes. The quantity and the quality of fruits resulting from the pollination of this bee did not differ significantly (P > 0.05) from the hand pollination. It was concluded that M. subnitida did not adapt to the mini watermelon cultivation in protected environment with cooling system, and the introduction of Scaptotrigona sp. nov. colonies, for pollination purposes, is viable in the commercial exploitation of seeded and seedless mini watermelon under greenhouse conditions.
O objetivo desta tese foi investigar a viabilidade da utilização dos meliponíneos, jandaíra (Melipona subnitida) e Scaptotrigona sp. nov. na polinização e produção de frutos de minimelancia (Citrullus lanatus) com e sem semente, sob cultivo protegido. Para tanto, foram investigados a biologia floral, os requerimentos de polinização das variedades de minimelancia, bem como o comportamento de adaptação e forrageamento, e a eficiência de polinização desses meliponíneos sob ambiente protegido. O experimento foi conduzido em uma casa de vegetação localizada no município de Fortaleza-CE. Os resultados revelaram que as variedades estudadas eram plantas monóicas com flores díclinas, e que o estigma de suas flores pistiladas permaneceu receptivo durante toda antese, a qual foi de 05:25 h às 14:20 h. As variedades com semente produziram frutos por meio da geitonogamia e da xenogamia, dentro da mesma variedade e entre diferentes genótipos diplóides. Diferentemente, os genótipos sem semente apenas formaram frutos por meio da polinização cruzada com pólen proveniente de variedades com semente. A abelha jandaíra não demonstrou nenhum interesse pela cultura diante das condições experimentais. Por outro lado, a abelha Scaptotrigona sp. nov. se adaptou bem ao confinamento e coletou recursos florais desde o segundo dia após sua introdução. Scaptotrigona sp. nov. mostrou comportamento essencial para a polinização da minimelancia, pois visitou, para coleta direta de néctar, flores estaminadas e pistiladas tanto dos genótipos diplóides quanto dos genótipos triplóides. A quantidade e qualidade dos frutos resultantes da polinização por essa abelha não diferiram significativamente da obtida pela polinização manual (P > 0,05). Conclui-se que a espécie de abelha M. subnitida não se adaptou ao cultivo de minimelancia em ambiente protegido com sistema de arrefecimento, e que a introdução de colônias Scaptotrigona sp. nov., para fins de polinização, é viável na exploração comercial da minimelancia com e sem semente em ambiente protegido.

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Myers, Marleen. "Molecular genetics of the floral response in Xerophta humilis." Master's thesis, University of Cape Town, 2008. http://hdl.handle.net/11427/4306.

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Boyd, Amy Elizabeth. "Evolution of floral traits: Biogeography, pollination biology and phylogenetics in Macromeria viridiflora." Diss., The University of Arizona, 2001. http://hdl.handle.net/10150/279781.

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Macromeria viridiflora is an herbaceous perennial in which floral traits vary geographically. In my dissertation research, I analyzed geographic variation in plant morphology and pollinator assemblages. I conducted experiments to determine the breeding system of the plants, and used visitation rate and pollen deposition to compare effectiveness of floral visitors as pollinators. I analyzed aspects of pollinator attractants and rewards in the flowers and placed this into the context of pollinator syndromes. In addition, I used phylogenetic analysis of the genus to determine polarity of change in corolla size within the species. Analysis of morphometric data from eight sites across the range of the species revealed significant among-population variation in vegetative and floral traits. Flower size variation is particularly strong and follows a latitudinal cline. Hawkmoths and hummingbirds were the main floral visitors throughout the range. The large-bodied hummingbirds visiting plants in the southern regions are not present in the northern regions, where flowers are visited by hummingbirds with barely half the body size and much shorter bills. This difference in bill size of hummingbird pollinators mirrors the geographic variation in flower size in M. viridiflora, suggesting that pollinator-mediated selection may be acting upon the species. Flowers of M. viridiflora have several characteristics that fit both the hummingbird and hawkmoth pollinator syndromes, namely copious sucrose-rich nectar and long floral tubes. However, they also have characteristics that correspond with a single major pollinator. This plant therefore presents a compromise floral syndrome that attracts two classes of pollinators. Breeding system studies showed that whereas plants are self-compatible and occasionally produce seed autogamously, pollinators are important for reproductive success in the plants. Combining visitation rate and pollen deposition as measures of pollinator effectiveness, hummingbirds were found to be the most effective pollinators at both sites. Phylogenetic analysis produced a single most parsimonious tree that supports the monophyly of the genus. Mapping of corolla size onto the phylogeny indicates that floral size has changed many times within the genus, and that very large corolla size in southern populations of Macromeria viridiflora has been derived from a smaller-flowered ancestor.

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Mickeliunas, Ludmila. "Biologia floral e reprodutiva e anatomia do labelo de Cyrtopodium polyphyllum Vell. (Orchidaceae, cyrtopodiinae)." [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315389.

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Orientador: Marlies Sazima
Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: O gênero Cyrtopodium Schltr. apresenta cerca de 42 espécies, sendo que 28 delas ocorrem no Brasil. Entre essas espécies Cyrtopodium polyphyllum Vell. (Sinônimo: Cyrtopodium paranaense Schltr.) ocorre, principalmente, na região litorânea do sul e sudeste brasileiros. As duas populações estudadas ocorrem na planície litorânea de Picinguaba e Praia da Fortaleza, Ubatuba-SP. Em ambas as regiões foram estudadas a fenologia, a morfologia floral, a anatomia do labelo, bem como observados os visitantes florais e identificados os mecanismos de polinização de C. polyphyllum. Também foram feitos experimentos para verificar o sistema reprodutivo da espécie. A quantidade de sementes potencialmente viáveis obtidas em cada tratamento, bem como a taxa de frutificação em ambiente natural foram determinadas para avaliar o sucesso reprodutivo. Os resultados dos estudos anatômicos foram comparados com os de outra espécie de Cyrtopodiinae, Grobya amherstiae Lindl. Cyrtopodium polyphyllum não oferece recursos aos seus polinizadores, que são atraídos às flores por engano. Cyrtopodium polyphyllum ocorre simpatricamente e, aparentemente, mimetiza flores de outras espécies que oferecem recursos, como Crotalaria incana (Fabaceae) e Stigmaphyllon sp. (Malpiguiaceae). Além da polinização por fator biótico, algumas flores de C. polyphyllum são autopolinizadas por gotas de chuva, um mecanismo que até então não havia sido descrito para Cyrtopodiinae. Cyrtopodium polyphyllum é autocompatível, mas dependente de polinizadores para a transferência de pólen. O grau de autocompatibilidade varia bastante entre as populações estudadas. Os frutos formados através das autopolinizações manuais, das polinizações cruzadas, assim como os desenvolvidos em condições naturais, apresentam alta taxa de sementes potencialmente viáveis e algumas exibem poliembrionia. Um estudo anatômico das glândulas florais presentes no labelo de Cyrtopodium polyphyllum e Grobya amherstiae foi efetuado com o propósito de relacionar a função desempenhada por estas estruturas com o processo de polinização. Ambas as espécies apresentam osmóforos, estruturas responsáveis pela produção dos odores característicos de cada espécie. Cyrtopodium polyphyllum possui dois tipos de osmóforos: um composto por papilas unicelulares, distribuídas pela superfície adaxial do labelo, e outro composto por emergências pluricelulares, presentes na região do calo do labelo. Em G. amherstiae os osmóforos são compostos por uma única camada de células epidérmicas, e ocorrem em toda a superfície abaxial do labelo. Grobya amherstiae apresenta, ainda, elaióforos, sendo um no ápice do labelo e outro na base da coluna. O elaióforo do ápice do labelo é de estrutura mista, composto por tricomas unicelulares glandulares e epiderme em paliçada, enquanto o da base da coluna é tricomáceo, apresentando apenas tricomas glandulares. Além de osmóforos e elaióforos, G. amherstiae apresenta também um guia de óleo na superfície adaxial do labelo formado por células papilosas
Abstract: The genus Cyrtopodium comprises about 42 species, with 28 occurring in Brazil. Among these species, Cyrtopodium polyphyllum (Synonym: Cyrtopodium paranaense Schltr.) occurs mainly on sandy soils in ¿restinga¿ vegetation along the coast of south and southern of Brazil. Large populations are found in the Natural Reserve of Picinguaba and at Praia da Fortaleza, municipality of Ubatuba, State of São Paulo, regions where this specie was studied. In both study sites were studied the phenology, floral morphology, lip anatomy, as well as recorded the floral visitors and identified the pollination mechanisms of C. polyphyllum. Also were performed treatments to verify the reproductive system of this specie. The quantity of potentially viable seeds obtained in each treatment, as well as the fruit set in natural habitat was recorded in order to evaluate the reproductive success. The results of the anatomic studies were compared with other Cyrtopodiinae specie, Grobya amherstiae Lindl. Cyrtopodium polyphyllum offers none reward to their pollinators, which are attracted to flowers by deceit. Cyrtopodium polyphyllum occur sympatrically and apparently mimicry flowers of other reward producing species, as Crotalaria incana (Fabaceae) and Stigmaphyllon sp. (Malpiguiaceae). Besides of the pollination by a biotic factor, some flowers of C. polyphyllum are pollinated by raindrops, a pollination mechanism not described to Cyrtopodiinae yet. Cyrtopodium polyphyllum is selfcompatible but pollinator-dependent. The tax of self-incompatibility is different between the two studied populations. The fruits formed from manual self-pollinations, crosspollinations, as well as developed under natural conditions, show an elevated tax of potentially viable seeds and sometimes present poliembriony. An anatomical study of the floral glands gifts in lip of Cyrtopodium polyphyllum and Grobya amherstiae were performed with the intention to relate the function played for these structures with the pollination process. Both species presents osmophores, structures responsible by the production odors, which are characteristic of each species. Cyrtopodium polyphyllum presents two types of elaiophores: one is composed by unicellular papillae, distributed along adaxial surface of the lip, and other composed by multicellular papillae arranged on the lip callous. In G. amherstiae the osmophores are composed by a singular layer of epidermic cells, and occurs along of all abaxial surface of the labellum. Grobya amherstiae also presents an elaiophore on the lip apices and on column basis. The elaiophore of the lip apices is a mixed structure, composed by unicellular glandular trichomes and a paliçade epidermis, although the elaiophore of the column basis is trichomatic, presenting only glandular trichomes. Besides of osmophores and elaiophores, G. amherstiae also presents an oil guide on adaxial surface of the labellum made by papillose cells
Mestrado
Mestre em Biologia Vegetal

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Mal, Tarun K. "Population biology and floral variation in Lythrum salicaria, a heterostylous colonizing weed." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ30286.pdf.

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Fuss, Alison Margot. "The floral biology of banksias in relation to crop production and management." Title page, contents and summary only, 1990. http://web4.library.adelaide.edu.au/theses/09PH/09phf994.pdf.

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Veley, Kira M. "Genetic and molecular interactions of the autonomous floral-promotion pathway in Arabidopsis thaliana." [Bloomington, Ind.] : Indiana University, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3380135.

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Thesis (Ph.D.)--Indiana University, Dept. of Biology, 2009.
Title from PDF t.p. (viewed on Jul 20, 2010). Source: Dissertation Abstracts International, Volume: 70-12, Section: B, page: 7374. Adviser: Scott D. Michaels.

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Thompson, Jessica Sara. "Pollination Biology of Ailanthus altissima (Mill.) Swingle (Tree-of-Heaven) in the Mid-Atlantic United States." Thesis, Virginia Tech, 2008. http://hdl.handle.net/10919/32586.

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To date little information has been collected on the pollination biology of Ailanthus altissima (Mill.) Swingle (tree-of-heaven), an invasive exotic in the U.S. This study was conducted to determine the insect pollinator fauna visiting A. altissima and to study general pollinator visitation patterns associated with the treeâ s nectar profile. A list of taxa visiting trees within each of three sites was developed from collected insects. Overall, visitor assemblage was dominated by the soldier beetle Chauliognathus marginatus with large numbers of ants in the genera Formica, Prenolepis, and Camponotus. No major diurnal pattern was found for visitation of insect pollinators using instantaneous counts. The nectar composition, concentration, and amount of total sugars in the flowers of A. altissima and how these are related to tree gender and time of day were determined. Nectar was found to be sucrose-dominant with lower, but nearly equal amounts of fructose and glucose. Total amounts of sugar in male and female blossoms were not statistically different, however higher concentrations of sugar were found in males (40.7%) than in females (35.3%). No difference was found over time. Nectar production and removal in trees was studied by comparing bagged flowers with flowers open to insect visitation. Bagged flowers were higher in overall sugar than open flowers, however, this was not constant across all times and gender.
Master of Science in Life Sciences

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Huber, Gwendolyn. "An investigation of highbush blueberry floral biology and reproductive success in British Columbia." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/57769.

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Vaccinium corymbosum (highbush blueberry) is and economically important crop in British Columbia and suffers from inconsistent and often inadequate pollination by the managed pollinator, the honeybee. The outcrossing strategy of blueberry requires a pollen vector; however honeybees are inefficient and choosey. One goal of this research was to investigate the variable attractiveness of blueberry flowers to pollinators, specifically focusing on odour, and the possibilities for improved pollination. Highbush blueberry flowers emit a wide range of volatile compounds that show heritability in the broad-sense. However, determining which compounds are important to pollinators is a challenging task and remains poorly understood. Pollinator choice was examined through monitoring of pollinators as well as determination of pollen movement through paternity analysis. There was no agreement between the analyses, which show that the genotypes Duke and Reka appear to attract more pollinators but the most common fathers are Bluecrop and Elliott. A second goal of this research was to examine reproductive success; and the discrepancy between attraction and reproduction could be due in part to the range in fertility observed among highbush blueberry genotypes. Inbreeding depression due to the use of a narrow breeding pool to develop genotypes is a potential cause of the observed range in fertility and offspring vigour. The degree to which inbreeding will increase homozygosity depends on the inheritance pattern of the tetraploid highbush blueberry; however, the current levels of inbreeding limit an accurate description of the inheritance pattern.
Forestry, Faculty of
Graduate

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Krause, Leonhard. "Floral biology, flowering phenology and floral visitors of five insect-pollinated tree species in a tropical lowland rainforest remnant of Pernambuco, Brazil." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-65292.

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West, Patricia A. "Floral richness, phytogeography, and conservation on islandsin Bahia de Los Angeles, Baja California, Mexico." Thesis, The University of Arizona, 2002. http://hdl.handle.net/10150/278789.

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Planners expect infrastructure development underway in Baja California to launch a wave of tourism. Managers will need information concerning the natural environment. This study focuses on the floral richness of the islands of Bahia de los Angeles, Baja California, Mexico. Results include island plant species inventories; a phytogeographic analysis evaluating the effects of island characteristics on plant species richness; a current human impact analysis, on floral richness; an evaluation of threats to floral richness and environmental health, including a non-native plant analysis; and management recommendations for preventing depletion of the native plant species richness on these islands from increased visitation. Floral richness ranges from 2 to 74 species per island. The best predictors of floral richness are the island area and the seabird presence or breeding. I recommend a combination of careful monitoring, dissemination of educational materials, and increased enforcement of current restrictions on island use to minimize human impacts.

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SILVA, JÚNIOR Cláudio Gomes da. "Polinização na caramboleira (Averrhoa carambola L.) por Apis mellifera L. : requerimentos da cultura e eficiência do polinizador." Universidade Federal Rural de Pernambuco, 2016. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6313.

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This study aimed to evaluate the floral biology, the pollinators, pollination requirements and pollinating efficiency of Apis mellifera L. bee in star fruit tree. The survey was conducted between the months of December 2015 to March 2016 in an orchard belonging to the Agronomic Institute of Pernambuco, municipality of Brejão, Agreste Pernambucano. The results showed that the flower anthesis took place from 07:00, but only to 08:00 all the flowers were open. The flower carambola presented longevity just one day, and its receptive stigma throughout the period that the flower remained open. six families insects were observed, but there was a predominance of bee species Trigona sp. and Apis mellifera. These bees foraged during the day, and Trigona sp. looking mainly resin in floral peduncle and A. mellifera exclusively for nectar. Honeybees spent on average 4,26 seconds to collect nectar and Trigona sp. spending an average of 24,64 seconds on each flower to collect the same resource. Regarding pollination requirements, studies have shown that there was initial fruit set in all forms of employed pollinations (free, restricted to paper, restricted with tulle and with a single visit of A. mellifera). However, a visit by A. mellifera, avenged (p<0,05) more fruit than other pollinations tests. Although several taxa of insects visit the flowers of star fruit, A. mellifera promoted greater (p<0,05) fruit set that all employees pollination treatments, presenting behavior efficient pollinator of that culture, to mitigate the pollination deficit and maximize fruit production.
Objetivou-se avaliar a biologia floral, os visitantes florais, os requerimentos de polinização e a eficiência polinizadora da abelha Apis mellifera L. em caramboleiras. A pesquisa foi conduzida entre os meses de dezembro de 2015 à março de 2016 em pomar, pertencente ao Instituto Agronômico de Pernambuco, município de Brejão, Agreste Pernambucano. Os resultados mostraram que a antese das flores aconteceu a partir das 07:00h, porém somente às 08:00h todas as flores se encontravam abertas. A flor da carambola apresentou longevidade de apenas um dia, tendo o seu estigma receptivo durante todo o período que a flor permaneceu aberta. Foram observados insetos de seis famílias, porém houve predomínio das espécies de abelhas Trigona sp. e Apis mellifera. Essas abelhas forragearam durante o dia, sendo Trigona sp. em busca principalmente de resina no pedúnculo floral e a A. mellifera exclusivamente por néctar. As abelhas A. mellifera gastaram em média 4,26 segundos para coletar néctar e a Trigona sp. gastava uma média de 24,64 segundos em cada flor para coletar o mesmo recurso. Em relação aos requerimentos de polinização, os estudos mostraram que houve vingamento inicial de frutos em todas as formas de polinizações empregadas (livre, restrita com papel, restrita com filó e com uma única de visita de A. mellifera). No entanto, uma visita pela A. mellifera, vingou significadamente (p<0,05) mais frutos que as demais polinizações empregadas. Apesar de vários táxons de insetos visitarem as flores da caramboleira, A. mellifera promoveu maior (p<0,05) vingamento de frutos que todos os tratamentos de polinização empregados, apresentando comportamento de eficiente polinizador dessa cultura, visando mitigar o déficit de polinização e maximizar a produção de frutos.

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Wunnachit, Wijit. "Floral biology of cashew (Anacardium occidentale L.) in relation to pollination and fruit set." Adelaide Thesis (Ph.D.) -- University of Adelaide, 1991. http://hdl.handle.net/2440/21622.

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Title page, contents and abstract only. The complete thesis in print form is available from the University Library.
Thesis (Ph.D.)--University of Adelaide, Dept. of Horticulture, Viticulture and Oenology, 1991

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Oyegbile, Maria Bolatito. "Phenotypic and genotypic variations in floral traits that affect mating system of Collinsia heterophylla." Thesis, University of Portsmouth, 2011. https://researchportal.port.ac.uk/portal/en/theses/phenotypic-and-genotypic-variations-in-floral-traits-that-affect-mating-system-of-collinsia-heterophylla(52132ee6-ce94-46b5-ac75-13c4ee73f196).html.

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This research studied phenotypic and genetic variations in eight floral morphological traits that affect the mating systems of Collinsia heterophylla, effects of pollination treatments on flower longevity and pollen-tube growth rate. The aim was to assess how phenotypic and genetic variations in floral traits, as well as other interacting mechanisms, influence mating systems in plants. This study found that, floral traits varied continuously within - and between - populations, and across floral developmental stages in Norway and Chiltern populations. Floral traits showed high correlations and heritabilities, with corolla, stamen and pistil recording the highest correlations in the two populations. Thereby, suggesting genetic linkages or pleiotropy effects among traits. Consequently, traits either evolve together or the selection of one trait constrains the other. Thus, the termination of pistil life through pollination and fertilisation could impact on structure and functions of the corolla, pistil and the stamens. The study also found that, the effects of pollination treatments, time of pollen arrival and pollination significantly affect flower longevity. Furthermore, autonomous selfing occurred early in Norway population, but late in Chiltern population. Inter-population cross pollination treatment showed shortest flower longevity in Norway population than Chiltern, indicating differential pollen-tube growth rate. Pollen-tube growth rate was assessed in-vitro and in-vivo and results showed no correlation in pollen-tube growth rate invitro and in-vivo. However, Chiltern population had longer pollen-tube growth in-vivo than Norway population. Similarly, Chiltern population grew longer pollen-tubes on the Norway style than on the Chiltern and vice versa in Norway pollen-tubes; suggesting partial cryptic selfincompatibility (CSI) in C. heterophylla. Therefore, floral traits variations, correlations, heritabilities, flower longevity and post-pollination processes can drive the course of mating systems evolution in flowering plants.

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Massoni, Julien. "Phylogeny, molecular dating and floral evolution of Magnoliidae (Angiospermae)." Phd thesis, Université Paris Sud - Paris XI, 2014. http://tel.archives-ouvertes.fr/tel-01044699.

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Deep phylogenetic relationships in the angiosperms had long been uncertain. However, by the end of the 1990s, large-scale studies contributed to the current well resolved picture of the tree of flowering plants, in which eudicots, monocots, and magnoliids are the three largest clades. Whereas monocots and eudicots have been recognized since the very first phylogenetic analyses, the monophyly of magnoliids (Canellales, Laurales, Magnoliales, and Piperales) is a more recent result. Magnoliidae, as now circumscribed, consist of 20 families and ca. 10,000 species mostly distributed in the tropics. Before the present thesis, several parts of the magnoliid tree had been well studied, but little was known about the evolutionary history of Magnoliidae as a whole. The first chapter of this thesis is a phylogenetic study conducted to clarify the relationships among families and orders of Magnoliidae. To do so, I sampled 199 species of Magnoliidae and 12 molecular markers from the three genomes and conducted phylogenetic analyses using parsimony, maximum likelihood, and Bayesian methods. The results confirm, with a greater level of support, two clades in Magnoliidae: Canellale + Piperales, and Laurales + Magnoliales. In addition, the relationships among the 20 families are generally well supported, and Lactoridaceae and Hydnoraceae are nested within Aristolochiaceae (Piperales). In the second chapter, the ages and phylogenetic positions of 10 fossils attributed to Magnoliidae were reviewed in detail. The goal of this study was to provide new reliable calibration points in order to conduct molecular dating analyses. These fossils were selected from the rich fossil record of the group because of their previous inclusion in phylogenetic analyses with extant taxa. The resulting calibration scheme provides six solid, internal minimum age constraints. The third chapter includes molecular dating analyses using the present calibration scheme and the same molecular dataset of Chapter 1. This study tends to push back in time the ages of the crown nodes of Magnoliidae (127.1-198.9 Ma), and of the four orders, Canellales (126.3-141.0 Ma), Piperales (88.2-157.7 Ma), Laurales (111.8-165.6 Ma), and Magnoliales (115.0-164.2 Ma). In the same chapter, I investigated the mode of diversification in the group. The strongly imbalanced distribution of species appears to be best explained by models of diversification with 6 to 14 diversification rate shifts. Finally, in the last chapter, I traced the evolution of 26 floral characters to reconstruct the ancestral flowers in key nodes of Magnoliidae. I used the phylogeny of Chapter 1 and an exemplar approach. Our results show that the most recent common ancestor of all Magnoliidae was a tree bearing actinomorphic, bisexual flowers with a differentiated perianth of two alternate, trimerous whorls of free perianth parts (outer and inner tepals) and probably three free stamens. This work provides key results on the evolution of Magnoliidae and raises several new questions such as the impact of geological crises on diversification of the group or the influence of pollinators and the environment on the evolution of floral morphology.

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馬肇偉 and Shiu-wai Ma. "Floral structure and reproductive biology of aegiceras (aegicerataceae), embelia (myrsinaceae) and maesa (maesaceae) in HongKong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31226498.

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Bomfim, Isac Gabriel AbrahÃo. "Uso de abelhas sem ferrÃo (Meliponinae:Apidae) em casa de vegetaÃÃo para polinizaÃÃo e produÃÃo de frutos com e sem semente de minimelancia [Citrullus lanatus (Thunb.) Matsum. &Nakai]." Universidade Federal do CearÃ, 2013. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9668.

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Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico
O objetivo desta tese foi investigar a viabilidade da utilizaÃÃo dos meliponÃneos, jandaÃra (Melipona subnitida) e Scaptotrigona sp. nov. na polinizaÃÃo e produÃÃo de frutos de minimelancia (Citrullus lanatus) com e sem semente, sob cultivo protegido. Para tanto, foram investigados a biologia floral, os requerimentos de polinizaÃÃo das variedades de minimelancia, bem como o comportamento de adaptaÃÃo e forrageamento, e a eficiÃncia de polinizaÃÃo desses meliponÃneos sob ambiente protegido. O experimento foi conduzido em uma casa de vegetaÃÃo localizada no municÃpio de Fortaleza-CE. Os resultados revelaram que as variedades estudadas eram plantas monÃicas com flores dÃclinas, e que o estigma de suas flores pistiladas permaneceu receptivo durante toda antese, a qual foi de 05:25 h Ãs 14:20 h. As variedades com semente produziram frutos por meio da geitonogamia e da xenogamia, dentro da mesma variedade e entre diferentes genÃtipos diplÃides. Diferentemente, os genÃtipos sem semente apenas formaram frutos por meio da polinizaÃÃo cruzada com pÃlen proveniente de variedades com semente. A abelha jandaÃra nÃo demonstrou nenhum interesse pela cultura diante das condiÃÃes experimentais. Por outro lado, a abelha Scaptotrigona sp. nov. se adaptou bem ao confinamento e coletou recursos florais desde o segundo dia apÃs sua introduÃÃo. Scaptotrigona sp. nov. mostrou comportamento essencial para a polinizaÃÃo da minimelancia, pois visitou, para coleta direta de nÃctar, flores estaminadas e pistiladas tanto dos genÃtipos diplÃides quanto dos genÃtipos triplÃides. A quantidade e qualidade dos frutos resultantes da polinizaÃÃo por essa abelha nÃo diferiram significativamente da obtida pela polinizaÃÃo manual (P > 0,05). Conclui-se que a espÃcie de abelha M. subnitida nÃo se adaptou ao cultivo de minimelancia em ambiente protegido com sistema de arrefecimento, e que a introduÃÃo de colÃnias Scaptotrigona sp. nov., para fins de polinizaÃÃo, Ã viÃvel na exploraÃÃo comercial da minimelancia com e sem semente em ambiente protegido.
The aim of this thesis was to investigate the viability of using the stingless bees, Melipona subnitida and Scaptotrigona sp. nov. for pollination and fruit production of seeded and seedless mini watermelon (Citrullus lanatus) under greenhouse conditions. To this end, the floral biology and pollination requirements of seeded and seedless mini watermelon varieties were investigated, as well as the adaptive and foraging behavior of both meliponines. The experiment was carried out in a greenhouse situated in the city of Fortaleza-CE. The results revealed that the varieties studied were monoecious plants with diclinous flowers, and that the stigma of its pistillate flowers remained receptive throughout the anthesis, which was from 05:25 h to 14:20 h. The seeded varieties set fruits by geitonogamous and xenogamous pollination, within the same variety and between different diploid genotypes. In contrast, the seedless genotypes set fruits only by cross-pollination with pollen from seeded varieties. M. subnitida did not show any interest in the crop, under the experimental conditions. On the other hand, Scaptotrigona sp. nov. adapted well to the confinement and collected floral resources since the second day after its introduction. Scaptotrigona sp. nov. showed an essential behavior for the pollination of mini watermelon, as they visited, for direct collection of nectar, staminate and pistillate flowers of both diploid and triploid genotypes. The quantity and the quality of fruits resulting from the pollination of this bee did not differ significantly (P > 0.05) from the hand pollination. It was concluded that M. subnitida did not adapt to the mini watermelon cultivation in protected environment with cooling system, and the introduction of Scaptotrigona sp. nov. colonies, for pollination purposes, is viable in the commercial exploitation of seeded and seedless mini watermelon under greenhouse conditions.

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Khan, Ayesha. "Composition, Diversity, Abundance, and Spatial Variation in the Floral Nectar Mycobiome of Rhododendron catawbiense and Lobelia cardinalis." Digital Commons @ East Tennessee State University, 2021. https://dc.etsu.edu/honors/615.

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Plant nectar microbiome, primarily consisting of fungi and bacteria, can qualitatively and quantitively alter the floral rewards by exploiting the chemical composition of the nectar which can thus impact plant-pollinator interactions, and ultimately affect plant reproductive success. Evidence suggests that changes in the microenvironmental conditions across various spatial gradients can induce changes in the floral nectar microbiome, which can account for microbial variation not only across plant species, but also within. Furthermore, nectar microbiome is also contingent on the pollinator systems and nectar quality. In this study, we used Rhododendron catawbiense (bee-pollinated; highly toxic nectar) and Lobelia cardinalis (hummingbird-pollinated; mildly toxic nectar) to explore the so-far unknown composition, diversity, and abundance of the nectar mycobiome across the spatial gradients of elevation and distance, respectively. It was done in the native Appalachian region of East Tennessee to improve the understanding of the microbiome-pollinator dynamics so that biodiversity can be better preserved. Through field sample collection, nectar harvesting, plating, isolation, DNA extraction, and Sanger Sequencing, we found that there were significant differences in terms of fungal abundance within the R. catawbiense and L. cardinalis species. Across plant species, R. catawbiense had more fungal abundance, attributable to being bee pollinated. L. cardinalis had more fungal diversity, attributable to its relatively low nectar toxicity. The results of Species Richness and Simpson’s Diversity, however, were found to be not significantly different in every instance. Furthermore, the plant species only shared two fungal species in common, with others also being unique to either an elevation or location, suggesting a high fungal species-plant species-spatial variation specificity.

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Ma, Shiu-wai. "Floral structure and reproductive biology of aegiceras (aegicerataceae), embelia (myrsinaceae) and maesa (maesaceae) in Hong Kong /." Hong Kong : University of Hong Kong, 2002. http://sunzi.lib.hku.hk/hkuto/record.jsp?B24534316.

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John-Bejai, Carus Kristoff Joel. "Wheat floral biology : prospects for improving the efficiency of hybrid seed production and abiotic stress tolerance." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/49925/.

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The modification of floral characteristics will be beneficial in improving the efficiency of hybrid seed production and the breeding of more climate resilient varieties in bread wheat (Triticum aestivum). Methods for phenotyping floral traits were initially tested using small genotype panels under controlled conditions and in field trials. Low-tech phenotyping methods appropriate for use by breeders and researchers were developed and demonstrated to be accurate. A panel of 111 genotypes was subsequently assessed in field trials using these methods. A high level of genotypic variation was observed for anther extrusion, anther length and anthesis duration/pattern and phenotypes were found to be stable across trials. Using this phenotypic data set, floral trait marker-trait associations (MTAs) were detected by association mapping and additional anther extrusion quantitative trait loci (QTLs) have been detected by linkage mapping in a bi-parental population. The phenotypic effects of candidate loci co-localizing with MTAs and QTLs were investigated using TILLING mutants and modifications to floral characteristics have been observed in some mutant lines. The utilization of phenotyping and genomic resources described in the present study is discussed and areas of future research have been identified.

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Santos, Jean Miguel Alves dos. "Visitantes florais e polinização de Tecoma stans (Bignoniaceae): efeito da pilhagem de néctar na eficácia reprodutiva." Universidade Federal da Paraíba, 2016. http://tede.biblioteca.ufpb.br:8080/handle/tede/9718.

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Bees are the main pollinators of plant species of agricultural system and most terrestrial environments, interacting with native and exotic plants. Exotic plants are usually found in urban areas, like Tecoma stans, an introduced ornamental species in Brazil, native from the southern United States and northern Central America. In flowers with long tubular corolla as T. stans it is common to certain species of bees make openings/holes that enable access to the floral resources. However, as they do not enter the flower, these bees perform illegitimate visits, because they do not contact the reproductive structures of flowers, and thus rob the floral resources without pollinate the flowers. However, no studies on the robbing behavior in this species has been done. Studies show that the robbing behavior of pollen and nectar can benefit, be neutral or decrease the reproductive success of other plant species. Some studies have been published on the interaction of bees with this plant, mainly in the region south and southeast of Brazil. The objective of this research was to study the bee species assemblage that visit the flowers of Tecoma stans in two areas with different characteristics emphasizing the robbing behavior and its consequences. The study was carried out at the Campus I of the Universidade Federal da Paraíba (UFPB), João Pessoa - PB, and the Sítio Olho D'água, Alhambra - PB, during October 2014 and November 2015. All the manual pollination tests resulted in fruit yield while spontaneous self-pollination did not yield fruits, confirming that Tecoma stans is auto-compatible, but needs pollinators to transfer the pollen grains. After floral anthesis, the flowers last for two days and provide pollen for its visitors, an average of 4064.9±543.9 grains/anther; and nectar, mean production of 14.4±7.3μl/day, with an average concentration of sugar of 21.3±3,8% and 0.47±0.3mg of sugar/μl. 24 species of bees were recorded in UFPB and 21 species at the Sítio Olho D'água. The bees started foraging around 5:00h till 17:30h. Visits and visitors were more constant in UFPB than at the Sítio Olho D'água that showed greater variation during the day The number of visits and visitors were significantly higher in UFPB. Peak of visitors occurred in the flowers at 8.00h (average of 9.3±8.3 visitors/plant/day in UFPB. While at the Sítio Olho D'água there was a mean peak of visitors at 8:00h and 12:00h, average of 5.6±2.4 visitors/plant/day and 4.9±2,7 visitors/plant/day, respectively. The most abundant bee species were Trigona spinipes, T. fuscipennis, Partamona littoralis, Plebeia flavocincta and Xylocopa spp. in UFPB, and M. scutellaris and Augochlora spp. in Sítio Olho D'água. Eulaema nigrita, E. atleticana, Centris analis, C. fuscata, C. tarsata, C. aenea, Euglossa carolina, M. scutellaris and Melitoma segmentaria were considered effective pollinators. Apis mellifera, Augochlora sp., Ceratina chloris, C. maculifrons, Nannotrigona punctata, Partamona littoralis, Plebeia flavocincta, Trigona spinipes and T. fuscipennis were classified as occasional pollinators. Females of Xylocopa spp., Pseudaugochlora spp. and Trigona spinipes were primary nectar robbers. Trigona spinipes and Trigona fuscipennis were primary pollen robbers. In experiments in which the flowers received a barrier to prevent nectar robbing, the Reproductive Success of open-pollination with and without barrier were low and similar in both areas, suggesting that the nectar robbing did not influenced the production of fruits, refuting the hypothesis proposed. However, in later tests the Reproductive Success and Reproductive Efficacy in flowers was higher without barrier suggesting that the nectar robbing favored pollination supporting the hypothesis tested. This difference in results may be related to different periods of carrying out the tests, as well as differences in the abundance and pollinator composition in each area. Although some results showed that nectar robbing can affect negatively the fruit production of T stans, we still need more conclusive experiments.
As abelhas são as principais polinizadoras de espécies vegetais do sistema agrícola e da maioria dos ambientes terrestres, interagindo com plantas nativas e exóticas. As plantas exóticas geralmente são encontradas em áreas urbanas como Tecoma stans, espécie nativa da região sul dos Estados Unidos e norte da América Central, introduzida no Brasil para ornamentação. Por apresentar flores com corolas tubulares longas, é comum a realização de aberturas/orifícios por certas espécies de abelhas que possibilitam o acesso aos recursos florais de T. stans. Todavia, como não entram na flor, essas abelhas realizam visitas ilegítimas, pois não contatam as estruturas reprodutivas das flores, e realizam a pilhagem dos recursos florais sem efetuar a polinização das mesmas. Alguns estudos foram publicados sobre a interação das abelhas com essa planta, principalmente, na região sul e sudeste do Brasil. Entretanto, não foram realizados estudos sobre o comportamento de pilhagem nessa espécie. Estudos demonstram que a pilhagem de pólen e néctar pode favorecer, ser neutra ou diminuir o sucesso reprodutivo de outras espécies vegetais. O objetivo desta pesquisa foi estudar a apifauna visitante de Tecoma stans em duas áreas com características diferentes, enfatizando o comportamento de pilhagem e suas consequências. O estudo foi realizado no Campus I da Universidade Federal da Paraíba (UFPB), João Pessoa - PB, e no Sítio Olho D'água, Alhandra – PB, durante outubro de 2014 e novembro de 2015. Todos os testes de polinização manual resultaram na formação de frutos enquanto a polinização espontânea não gerou frutos, confirmando que Tecoma stans possui o sistema reprodutivo autocompatível, porém necessita de agentes polinizadores para a transferência dos grãos de pólen. Após a antese, as flores duram dois dias e oferecem pólen para seus visitantes, com média de 4.064,9±543,9 grãos/antera; e néctar, com produção média de 14,4±7,3μl/dia, com concentração média de açúcar de 21,3±3,8% e 0,47±0,3mg de acúcar/μl. Foram registradas 24 espécies de abelhas visitantes florais na UFPB e 21 espécies no Sítio Olho D’água. As abelhas iniciaram o forrageio por volta das 5:00h até às 17:30h. As visitas e os visitantes foram mais constantes na UFPB que no Sítio Olho D’água que apresentou maior variação durante os dias. O número de visitas e o número de visitantes foram significativamente maiores na UFPB. Ocorreu um pico de visitantes às 8:00h (média de 9,3±8,3 visitantes/planta/dia) na UFPB. Enquanto no Sítio Olho D’água ocorreu um pico médio de visitantes às 8:00h e outro às 12:00h (média de 5,6±2,4 visitantes/plantas/dia e 4,9±2,7 visitantes/planta/dia, respectivamente). As abelhas mais abundantes foram Trigona spinipes, T. fuscipennis, Partamona littoralis, Plebeia flavocincta e Xylocopa spp. na UFPB e Melipona scutellaris e Augochlora spp. no Sítio Olho D'água. Eulaema nigrita, E. atleticana, Centris analis, C. fuscata, C. tarsata, C. aenea, Euglossa carolina, Melipona scutellaris e Melitoma segmentaria foram consideradas polinizadoras efetivas; e Apis mellifera, Augochlora sp., Ceratina chloris, C. maculifrons, Nannotrigona punctata, Partamona littoralis, Plebeia flavocincta, Trigona spinipes e T. fuscipennis foram classificadas como polinizadoras ocasionais. As espécies de Xylocopa, Pseudaugochlora e Trigona spinipes foram pilhadoras primárias de néctar. Trigona spinipes e Trigona fuscipennis foram pilhadoras primárias de pólen. Em experimentos nos quais as flores receberam uma barreira para evitar a pilhagem de néctar, o sucesso reprodutivo da polinização livre em flores com e sem barreira foram baixos e semelhantes nas duas áreas, sugerindo que a pilhagem de néctar não influenciou na produção de frutos, refutando a hipótese proposta. Porém, em testes posteriores o Sucesso Reprodutivo e a Eficácia Reprodutiva nas flores sem barreira foi maior, sugerindo que a pilhagem de néctar favorece a polinização. Essa diferença nos resultados pode estar relacionada a diferentes períodos de realização dos testes, assim como a diferenças na abundância e composição de polinizadores em cada área. Os resultados mostraram que a pilhagem de néctar pode afetar a produção de frutos, porém são necessários experimentos mais conclusivos.

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Soule, Jacob W. "Heterochrony of floral and mating system characters between Nicotiana longiflora and N. plumbaginifolia." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/5001.

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Thesis (M.A.)--University of Missouri-Columbia, 2007.
The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on November 6, 2007) Includes bibliographical references.

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Nasario, João Paulo Sardin 1990. "Biologia floral, reprodutiva e cariótipos de espécies de Pseudobombax Dugand (Bombacoideae, Malvaceae) do sudeste do Brasil." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314934.

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Orientador: Eliana Regina Forni Martins
Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Tradicionalmente incluso na extinta família Bombacaceae, Pseudobombax Dugand pertence à subfamília Bombacoideae, Malvaceae sensu lato. Abrange cerca de 29 espécies, das quais 16 ocorrem em território brasileiro e nove são endêmicas. No Brasil, a maioria das espécies é encontrada no sudeste, planalto central e nordeste. As espécies possuem considerável valor comercial, por serem utilizadas no mundo todo, principalmente na ornamentação e arborização urbana. Estudos sobre o sistema reprodutivo e citogenéticos são raros para o gênero. Com este trabalho, objetivou-se o estudo da biologia floral e reprodutiva, assim como a análise cariotípica de três espécies de Pseudobombax da região sudeste do Brasil. As espécies de Pseudobombax são importantes fontes de pólen e néctar para seus visitantes florais, especialmente por florescerem na estação seca. Algumas diferenças foram observadas entre os períodos de floração e frutificação durante os dois anos de estudo, as quais podem estar relacionadas a diferentes quantidades de chuva antes do início da floração. As flores apresentam antese crepuscular/noturna, com características que se encaixam na síndrome da quiropterofilia. As flores de P. tomentosum são significativamente diferentes das demais espécies (maior comprimento da flor, das pétalas, do ovário e do cálice), porém a forma e o indumento dos frutos é a principal característica que podemos utilizar para separar taxonomicamente as três espécies. Os estudos reprodutivos das três espécies evidenciaram alta porcentagem de fecundação cruzada, indicando alogamia. A eficácia reprodutiva manteve-se alta, confirmando a necessidade de polinização cruzada e, consequentemente, de seus polinizadores. As análises citogenéticas mostraram contagens inéditas, com 2n=88 para Pseudobombax sp. (nova) e P. tomentosum e 2n=84 para P. grandiflorum. O número básico sugerido para as espécies de Pseudobombax é x=44. Foi confirmado mais de um número cromossômico no gênero, o que sugere a derivação por disploidia, decorrente de possíveis rearranjos cromossômicos. Pseudobombax sp. (nova) e P. grandiflorum, espécies muitas vezes confundidas entre si, possuem números cromossômicos diferentes (2n=88 e 2n=84, respectivamente) sendo este um caráter adicional importante na separação taxonômica das duas espécies. O bandamento CMA/DAPI evidenciou um padrão conservado dentro do gênero, com seis bandas CMA+ nas três espécies. Os valores métricos dos cromossomos das espécies em estudo indicaram um comprimento do complemento cromossômico pequeno, nas quais o menor tamanho cromossômico foi de 0,3 µm em P. grandiflorum e o maior foi de 3,5 µm em P. tomentosum. O diferente número cromossômico, bem como algumas características morfológicas florais e do fruto podem ser utilizadas na separação taxonômica das três espécies de Pseudobombax em estudo, evidenciando assim a existência de uma nova espécie para o gênero
Abstract: Traditionally included in the extinct family Bombacaceae, Pseudobombax Dugand belongs to the Bombacoideae subfamily, Malvaceae sensu lato. Composed by about 29 species, of which 16 occur in Brazilian territory and nine are endemic. In Brazil, most species are found in the southeast, northeastern and central plains. Species have considerable commercial value, for being used worldwide, especially in ornamentation and urban forestry. Studies on the reproductive system and cytogenetics are rare for the genus. This work aimed to study the floral and reproductive biology, as well as analysis of karyotype of three species of Pseudobombax from southeastern Brazil. The Pseudobombax species are important sources of pollen and nectar for their floral visitors, especially by flourishing in the dry season. Some differences were observed between the periods of flowering and fruiting during the two years of study, which may be related to different amounts of rainfall before flowering. The flowers have crepuscular/nocturnal anthesis, with features that fit in the chiropterophily syndrome. The flowers of P. tomentosum are significantly different from the other species (greater length of the flower petals, the ovary and the cup), but the shape and indumentum of the fruit is the main feature that we can use to separate the three species taxonomically. Reproductive studies of the three species showed a high percentage of outcrossing, indicating outcrossing. The reproductive efficiency remained high, confirming the necessity of cross-pollination and, therefore, their pollinators. Cytogenetic analysis showed unprecedented chromosome counts, with 2n=88 to Pseudobombax sp. (new) and P. tomentosum and 2n=84 for P. grandiflorum. The basic chromosome number suggested to Pseudobombax is x=44. We confirmed more than one chromosome number in the genus, which suggests derivation by disploidy, due to possible chromosomal rearrangements. Pseudobombax sp. (new) and P. grandiflorum, species often confused with each other, presented different chromosome numbers (2n=88 or 2n=84 , respectively) which is an important taxonomic character for the separation of the two species. The CMA/DAPI banding showed a conserved pattern within the genus, with six CMA+ bands in all species. The metric values of the chromosomes of the species under study indicated a small chromosomal complement length, wherein the smallest chromosome size was 0.3 µm in P. grandiflorum and the largest was 3.5 µm in P. tomentosum. The different chromosome numbers, as well as some floral and fruit morphological characteristics can be used for taxonomic separation of the three Pseudobombax species under study Pseudobombax, thus revealing the existence of a new species for the genus
Mestrado
Biologia Vegetal
Mestre em Biologia Vegetal

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Simons, Kristin Jean. "Cloning and characterization of the wheat domestication gene, Q." Diss., Manhattan, Kan. : Kansas State University, 2005. http://hdl.handle.net/2097/135.

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Thoenes, Steven Charles. "Influence of floral resources on honey bee colony growth and reproductive swarming patterns in the Sonoran Desert of Arizona." Diss., The University of Arizona, 1992. http://hdl.handle.net/10150/186019.

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Relationships between patterns of food (nectar and pollen) harvest and honey bee colony growth and reproduction are poorly understood. Research was conducted on the relationships between food harvest and colony growth in the Sonoran desert of Arizona. Annual patterns of resources (pollen harvest rates, pollen protein content, protein influx rates, and nectar harvest) and colony growth and reproduction (brood area, adult population, and reproductive swarm occurrence) for the Tucson area were obtained. Annual patterns were analyzed for periodicity and stability using autocorrelation time series analysis. Each resource pattern was compared to each colony growth pattern using cross-correlation analysis which revealed time lag interval of correlation between the two data sets. The foraging activity of the honey bee colonies was dominated by pollen foraging, with pollen harvested on a year-round basis. Nectar collection was limited to only a few weeks each year. The colony growth patterns all reached their maxima in spring or early summer. The autocorrelation analysis revealed that all data sets exhibited periodic and stable behaviors based on an annual (52 week) cycle. The colony growth data sets were also predictable in magnitude. The cross-correlation analysis revealed that the areas of sealed brood, number of adult bees, and swarm occurrence were more correlated to protein influx than any other food resource parameter. The timing of the greatest nectar harvest occurred just after the peak of swarm occurrence. Colonies fed supplementary pollen diets containing 24.8% or 17.2% protein were compared to non-fed controls. The number, timing, and survival of swarms was monitored. The initiation of queen-cell construction was directly related to protein influx rates exceeding 20 g colony⁻¹ day⁻¹. The 24.8% protein treatment swarmed earlier than the other two treatments. Swarm survival was highest in swarms that issued just prior to the nectar flow. Honey bee colony growth and reproductive swarming patterns in Tucson are directly related to protein influx rates. This response results in swarm production at a time of year when nectar is most abundant, thus aiding long-term swarm survival. Similar relationships between patterns of food harvest and swarming should occur for all temperate honey bee colonies.

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Barker, Daniel A. "The Effects of Invasive Plant Species on Pollen Transfer Networks in Southern Appalachian Floral Communities." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/asrf/2018/schedule/188.

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Approximately 90% of flowering plants depend on pollinators for reproduction. The stability and effectiveness of plant-pollinator interactions are crucial for ecosystem function. Increasing numbers of non-native plants are naturalized in plant communities and may alter pollination success of native plant species. Thus, invasive species have the potential to alter community function and stability. However, the effects of invasive species on community-wide plant-pollinator interactions are poorly understood. While the effects of invasive species on the structure of plant-pollinator networks are well studied. However, these studies have relied on pollinator visitation data that is only one component of the pollination process. Thus, the effects of invasive species on pollen transport and pollen transfer dynamics remain unknown and this may misrepresent the true nature of invasive effects on community-wide plant-pollinator interactions. Pollen transport networks may give more accurate representations of plant-pollinator interactions by providing information on pollen collection by floral visitors. Therefore, in this study I evaluate the effect of the invasive Cirsium arvense on pollen transport networks to improve our understanding of the impact that invasive plants have on community-wide plant-pollinator interactions. Pollinators were collected on one invaded and one non-invaded site once weekly throughout the flowering season (May- August of 2017). Pollen was isolated for each insect and pollen samples were identified with a pollen reference library and counted using a hemocytometer. 154 insect morphospecies were collected carrying 73 pollen species. Preliminary results indicate that Cirsium arvense has no impact on network structure: connectance (0.15 and 0.18), link density (3.01 and 2.23), and weighted nestedness (0.68 and 0.75), for invaded and non-invaded respectively. However, the role of individual species within the network seems to vary between sites suggesting that Cirsium arvense may change community dynamics (identity of species-pair interactions). Future analysis will evaluate invasive species effects at the species level.

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Maad, Johanne. "Selection and Floral Evolution in Platanthera bifolia and P. chlorantha (Orchidaceae)." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2002. http://publications.uu.se/theses/91-554-5226-4/.

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Daniels, Jesse. "Direct and indirect effects of invasive Cirsium arvense on pollination in Southern Appalachian floral communities." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/asrf/2018/schedule/124.

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To date, 13168 plant species have been naturalized outside their native range. While invasive plants efficiently compete for resources, they can also disrupt vital mutualisms. Pollination is a crucial mutualism required for 87% of flowering plants to reproduce. Invasive species may disrupt patterns of pollinator visitation, pollen transfer dynamics (conspecific [CP] and heterospecific [HP]), and reproductive success. Furthermore, whether invasive species’ effects depend on scale (site vs. patch) is not known. Thus, it is critical to understand the effects of invasive plants at all stages of pollination and how those effects vary with scale. Here, I investigate pollinator visitation, CP and HP deposition, and pollen tube growth between local flowers in sites and plots invaded by Cirsium arvense. For 7 weeks (June-July), pollinator visits were recorded and styles collected from an invaded and non-invaded floral community. Styles were processed for fluorescent microscopy. The number and identity of pollen grains on stigmas and the number of pollen tubes at style bases were recorded. The invaded site received significantly less visits than the non-invaded site, but the effect varied by species. This variation is not explained by local species’ abundance or floral symmetry. Similarly, invaded plots received significantly less visits than non-invaded plots, and no plot-species interaction was found. The invaded site received more CP and HP while invaded plots received less CP and HP. There was no difference in pollen tube growth between sites or plots. My preliminary results suggest that C. arvense affects pollinator visitation and pollen transfer dynamics in local floral communities, but the effects vary depending on species and scale. Conversely, these effects do not seem to lead to differential reproductive success between invaded and non-invaded communities at any scale.

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Hooi, Wei Yeng. "Search for early molecular markers of the mantled floral variation of oil palm." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS244.

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Titre du projet: Recherche de marqueurs moléculaires précoces de l’anomalie florale mantled du palmier à huile Objectifs : - identifier des marqueurs d’expression de la variation somaclonale mantled par comparaison entre les transcriptomes conformes et variants.- valider la capacité de discrimination des marqueurs sélectionnés lors des stades précoces du processus in vitro. Stratégie et Méthode: Analyse transcriptomique de l’inflorescence normale de palmier à huile et construction d’un transcriptome de référence. Technique : RNAseq, séquençage Illumina.Identification des séquences et voies de régulation d’intérêt. Technique: analyse bioinformatique des données de séquençage.Comparaison entre les trancriptomes issus d’inflorescences normales vs. mantled par re-séquençage de banques obtenues ) partir de différents génotypes clonaux. Technique : Illumina.Identification des séquences présentant de manière cohérente des profils d’expression dépendant du phénotype. Technique : analyse bioinformatique des données de séquençage, analyse statistique des profils d’expression. Validation des marqueurs candidats sur des paires de régénérants normal/mantled issus de lignées clonales variées, ainsi que sur des cultures in vitro à différents stades du processus de régénération. Technique : PCR quantitative (q-PCR)
Project title : Search for early molecular markers of the mantled floral variation of oil palmObjectives : - identifying expression markers of the mantled somaclonal variation through the comparison between the true-to-type and the variant transcriptome. - assessing the discriminating power of the selected markers at early stages of the in vitro process.Strategy and Methods : Transcriptomic analysis of the normal oil palm inflorescence, construction of a reference transcriptome. Technique : RNAseq, Illumina sequencing.Identification of sequences and pathways of interest. Technique : bioinformatic analysis of sequencing data.Comparison between the normal and the mantled inflorescence transcriptome through the re-sequencing of libraries generated from several different clonal lines. Technique : Illumina. Identification of sequences displaying consistently a phenotype-dependent differential expression pattern. Technique : bioinformatic analysis of sequencing data, statistical analysis of expression patterns. Validation of candidate markers on normal/mantled regenerant palm pairs from different clonal lines and on normal-/mantled-derived in vitro cultures at various stages of the industrial regeneration process. Technique : quantitative PCR (q-PCR)

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Zanina, Dalva Neta e. "Quem poliniza Cereus jamacaru?" reponame:Repositório Institucional da UFC, 2013. http://www.repositorio.ufc.br/handle/riufc/17133.

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ZANINA, Dalva Neta e. Quem poliniza Cereus jamacaru? 2013. 41 f. Dissertação (Mestrado em ecologia e recursos naturais)- Universidade Federal do Ceará, Fortaleza-CE, 2013.
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The Cereus jamacaru DC cactus is an important species for the Brazilian Northeast. Has developed many studies and uses of the plant, but has not been established which pollinator in areas of natural distribution. The aim was to identify the most effective pollinator for form and function C. jamacaru flower. Data collection was done in a fragment of Caatinga where we collected data on phenology, morphology and floral biology beyond search visitors. Fruits were numbered 695young flowers, 175 flowers and 21 fruits. The cactus flowering displays multiple bang type. The flowers are hermaphrodite. The hypanthium is tapered 6.5 cm length, 3 cm in diameter and 0.6 cm opening in the base. The nectariferous chamber is formed by the base of the stamens and has the opening with some kind of lock by the stylus. According to the morphological evidence comparing pollination by bats and moths observed most of the arguments in favor of chiropterophily. We simulate visits per Glossophaga soricina and got perfect compatibility, however we did not observe any animals visit with optimal fit. The visitor who came closest pollinator behavior was Xylocopa sp, a large bee. Probably this bee was responsible for the formation of the few fruits.
A cactácea Cereus jamacaru DC é uma espécie emblemática para o nordeste brasileiro. Tem se desenvolvido muitos estudos e usos da planta, mas não se tem estabelecido qual o polinizador em áreas de distribuição natural. O objetivo do trabalho foi identificar qual o polinizador mais efetivo para forma e função da flor de C. jamacaru. A coleta de dados foi feita em um fragmento de Caatinga onde foram coletados dados sobre fenologia, morfologia e biologia floral além de busca por visitantes. Foram contados 695 botões, 175 flores e 21 frutos. O mandacaru apresenta floração do tipo multiple bang. As flores são hermafroditas, protândricas, de simetria dorsoventral esternotríbica. O hipanto é cônico 6,5 cm de comprimento, 3 cm diâmetro na abertura e 0,6 cm na base. A câmara nectarífera é formada pela base dos estames e possui a abertura com uma espécie de fechadura pelo estilete. De acordo com indícios morfológicos ao comparar a polinização feita por morcegos e mariposas observou-se a grande maioria dos argumentos a favor de quiropterofilia. Simulamos visitas por Glossophaga soricina e obtivemos perfeita compatibilidade, porém não observamos visita de nenhum animal com encaixe perfeito. O visitante que mais se aproximou do comportamento polinizador foi Xylocopa sp, uma abelha de grande porte. Provavelmente esta abelha foi a responsável pela formação dos poucos frutos.

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Sayou, Camille. "Structure, fonction et évolution de LEAFY, facteur de transcription clé du développement floral." Phd thesis, Université de Grenoble, 2013. http://tel.archives-ouvertes.fr/tel-00949325.

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LEAFY (LFY) est un facteur de transcription central pour le développement des plantes, en particulier pour la floraison chez les angiospermes. LFY est très conservée, même chez les espèces ne portant pas de fleurs. On dispose de nombreuses données génétiques sur LFY et son réseau de régulation chez la plante modèle Arabidopsis thaliana, mais les mécanismes moléculaires impliqués dans son fonctionnement ne sont pas entièrement élucidés. LFY possède deux domaines conservés : un domaine de liaison à l'ADN et un domaine de fonction inconnue en position N-terminal. L'objectif a été de comprendre le rôle du domaine N-terminal et d'étudier l'évolution de la spécificité de liaison à l'ADN de LFY. Nous avons obtenu la structure cristallographique du domaine N-terminal de LFY et découvert qu'il s'agissait d'un domaine SAM (Sterile Alpha Motif) permettant l'oligomérisation de la protéine. Nous avons validé l'importance de cette propriété pour la fonction florale de LFY chez A. thaliana. Nous avons ensuite montré, par des analyses in vitro et in vivo en ChIP-seq que l'oligomérisation influençait la liaison à l'ADN en permettant une liaison coopérative sur plusieurs sites de liaison, en assurant la sélectivité de la protéine vis-à-vis de l'ADN et en permettant l'accès de la protéine à des régions génomiques où la conformation de la chromatine est normalement défavorable à la liaison. Cette étude intégrative a permis de mieux comprendre le fonctionnement de LFY. Des modifications dans les réseaux de régulation de l'expression des gènes sont source de nouveauté et d'évolution. LFY étant très conservée et ne faisant pas partie d'une famille multigénique, nous nous sommes demandé si sa spécificité de liaison à l'ADN avait évoluée. Nous avons montré que LFY était apparue chez les algues multicellulaires et que sa spécificité avait connue au moins deux changements majeurs au cours de l'évolution. Nous avons expliqué ces modifications au niveau moléculaire par des approches de biologie structurale et de biochimie. Nous avons identifié une espèce chez qui LFY a une spécificité relâchée et nous proposons qu'une telle forme ait pu permettre les transitions d'une spécificité à une autre.

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Dias, Letícia do Carmo Dutra. "Biologia reprodutiva de Aechmea bruggeri Leme (Bromeliaceae): uma espécie endêmica da Floresta Atlântica ameaçada de extinção." Universidade Federal de Juiz de Fora, 2014. https://repositorio.ufjf.br/jspui/handle/ufjf/710.

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CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Bromeliaceae compreende aproximadamente 3.172 espécies e é a quarta família de angiospermas com maior riqueza no domínio da Floresta Atlântica. Seus representantes desempenham um relevante papel ecológico, atuando em importantes processos biológicos nos ecossistemas onde ocorrem, tais como os relacionados à polinização e dispersão de sementes. Apesar da elevada importância ecológica, ainda existe pouco conhecimento sobre muitos aspectos da biologia reprodutiva da maioria das espécies de bromélias. Aechmea bruggeri é endêmica da Floresta Atlântica e foi enquadrada na categoria “Criticamente em Perigo” na última revisão da “Lista de Espécies Ameaçadas do Estado de Minas Gerais”. Os objetivos deste trabalho foram investigar aspectos da biologia reprodutiva de A. bruggeri, visando contribuir para o conhecimento da evolução de diferentes mecanismos na reprodução da família Bromeliaceae e para o desenvolvimento de estratégias de conservação e manejo das populações de A. bruggeri e de outras espécies endêmicas e ameaçadas de extinção. A espécie é autoincompatível e alógama, dependente de polinizadores para formação de frutos e sementes, e na área de estudo, apresenta alta taxa de frutificação sob condições naturais. Aechmea bruggeri é polinizada efetivamente por beija-flores da espécie Thalurania glaucopis. Este trabalho demonstrou que, localmente, as populações da espécie analisada são férteis e viáveis, apesar de estarem sujeitas a ação de predação de suas sementes por larvas de coleópteros.
Bromeliaceae Juss. presents approximately 3172 species and is considered the fourth family of Angiosperms with the largest species richness of the Atlantic domain. Their representatives play an important ecological role, acting in important biological processes in ecosystems where they occur, such as those related to pollination and seed dispersal. Despite the high ecological importance, there is still little knowledge about many aspects of the reproductive biology of most species of bromeliads. Aechmea bruggeri is endemic to the Atlantic Forest and was framed in category "Critically Endangered" in the latest revision of the "List of Threatened Species of the state of Minas Gerais." The aim of this study were investigate aspects of the reproductive biology of A. bruggeri, to contribute to the knowledge of the evolution of different mechanisms in the reproduction of the Bromeliaceae family and for developing strategies for conservation and management of populations of A. bruggeri and other endemic and endangered species. The species is self-incompatible and alogamous dependent on pollinators for fruit and seed, and in the study area, has a high rate of fruit set under natural conditions. Aechmea bruggeri is effectively pollinated by hummingbirds species of Thalurania glaucopis. This study demonstrated that, locally, the populations of the species analyzed are viable and fertile, although they are subject to the action of predation of seeds by larvae of beetles.

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Filho, AlÃpio JosÃ de Souza Pacheco. "Ecologia da polinizaÃÃo e biologia reprodutiva de Ipomoea bahiensis Willd. no semi-Ãrido brasileiro." Universidade Federal do CearÃ, 2010. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=6930.

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CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior
O estudo da ecologia floral Ã fundamental para o entendimento das relaÃÃes entre flores e seus visitantes, assim como para entender o papel dessa interaÃÃo no sucesso reprodutivo e na manutenÃÃo da populaÃÃo vegetal. No presente estudo, investigou-se a morfologia, a biologia floral e o sistema de reproduÃÃo de Ipomoea bahiensis Willd., bem como as interaÃÃes entre suas flores e a guilda de visitantes florais. A investigaÃÃo teve como objetivo central entender tanto as relaÃÃes ecolÃgicas e evolutivas entre I. bahiensis e insetos antÃfilos quanto o sistema reprodutivo da planta. Para tanto, foram empregados mÃtodos para analisar a morfologia funcional das flores, a relaÃÃo da morfologia e biologia floral com os visitantes, o comportamento e frequÃncia desses animais, o requerimento polÃnico e a relaÃÃo fruto/flor e, por fim, foi quantificada a limitaÃÃo de pÃlen. Para incrementar o conhecimento sobre a ecologia floral, foram observados os visitantes de nectÃrios extranupciais e sua relaÃÃo com inimigos florais. A partir da anÃlise dos dados obtidos, observou-se que as flores de I. bahiensis possuem atributos predominantemente de psicofilia, contudo tÃm plasticidade no sistemas de polinizaÃÃo, podendo ser polinizadas por abelhas de pequeno porte. A dinÃmica do nÃctar juntamente com o perÃodo de receptividade do estigma procura, possivelmente, garantir a visitaÃÃo de borboletas nesse perÃodo. No entanto, a variaÃÃo na frequÃncia dos visitantes florais foi entendida a partir da disponibilidade de pÃlen, pois os principais visitantes foram abelhas coletoras de pÃlen. Os testes de polinizaÃÃo mostraram que o sistema de reproduÃÃo Ã xenÃgamo, dependente de vetores biÃticos e apresenta auto-incompatibilidade. TambÃm foi observado que a planta Ã limitada por pÃlen
The study of floral ecology is pivotal to understanding the relationships between flowers and their visitors, as well as to understand the role of this interaction in reproductive success and maintenance of plant population. In this study, it was investigated the morphology, floral biology and breeding system of Ipomoea bahiensis Willd. and the interactions between its flowers and the guild of floral visitors. The research aimed mainly to understand the ecological and evolutionary relationships between I. bahiensis and anthophilous insects. For this, methods were used to analyze the functional morphology of flowers, the relationship of floral morphology and biology with visitors, the frequency and behavior these animals, the requirement for pollen and the rate fruit/flower and, finally, was estimated pollen limitation. To increase knowledge about the floral ecology were observed extranupcial nectariesâs visitors and its relation to floral enemies. From the analysis of the data, it was observed that the flowers of I. bahiensis have attributes predominantly psycophily, however they present plasticity in pollination systems, and can also be pollinated by small bees. The dynamics of nectar herewith with the period of stigma receptivity seeks to ensure the visitation of butterflies in this period. However, the variation in the frequency of floral visitors was understood from the availability of pollen, because the main visitors were bees pollen collecting. Pollination tests showed that the reproductive system is xenogamous, dependent on biotic vectors and presents self-incompatibility. It was also observed that the plant is limited by pollen

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39

Pang, Chun-chiu, and 彭俊超. "Floral biology, pollination ecology and breeding systems of selected Dasymaschalon, Desmos, Pseuduvaria and Uvaria species (Annonaceae) inSouthern China and Australia." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B47752737.

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　　　The Annonaceae is a large, early-divergent family of angiosperms. Although the majority is pollinated by small beetles, there is a great diversity of floral morphologies. Evolutionary shifts of pollination mechanisms have occasionally been reported in disparate lineages including shifts to pollination by large beetles, flies, thrips, bees and cockroaches. It was previously hypothesized that floral morphological changes in different lineages are adaptive and correspond to evolutionary shifts of pollination mechanisms. This hypothesis is tested here by comparing selected species that have substantial morphological differences with their close relatives. 　Comprehensive studies of the floral biology of four Annonaceae species, Dasymaschalon trichophorum, Desmos chinensis, Pseuduvaria mulgraveana and Uvaria cordata, are presented. All are beetle-pollinated. Dasymaschalon trichophorum, D. chinensis and U. cordata were inferred to be self-compatible based on inter-simple sequence repeat marker data as there was evidence of significant gene flow and a low level of genetic differentiation between populations. This was corroborated for D. chinensis and U. cordata by experimental controlled pollination tests for geitonogamy, in which both were shown to set fruit. 　　　Similar floral phenological and pollination ecological results were obtained for D. chinensis and D. trichophorum despite significant differences in floral architecture, as the former has six petals (typical of most other Annonaceae species), whilst the latter only has three. The results suggested that the substantial change in floral morphology in these two closely-related genera is probably non-adaptive as there is no change in pollination system. It is hypothesised that the morphological change is likely due to the disruption of homeotic gene expression during floral organ development. 　　　Studies of floral phenology and pollination ecology of Uvaria cordata revealed that it has a 3-day flowering rhythm and is pollinated by small beetles. The pollination ecology is unexpectedly similar to other species with typical beetle-pollination syndromes, although it lacks a pollination chamber and has pale-colored petals. It is suggested that species that lack a pollination chamber are more likely to be pollinated by guilds other than beetles or thrips as a floral chamber increases pollinator specificity. Several morphological and phenological characters are also presumably correlated with the evolution of generalist pollination to increase the pollination efficiency and assure fruit production, including torus shape, petal orientation, stigma shape, petal color, carpel and/or ovule number and the overlap of pistillate and staminate phases. 　　　Pseuduvaria is unusual in the Annonaceae as the majority of species possess unisexual flowers. Most species were previously interpreted as having staminate and structurally pistillate flowers, with infertile staminodes in the latter. The ‘pistillate’ flowers of P. mulgraveana are shown to produce viable pollen, however, contradicting this hypothesis. It is therefore recommended that floral unisexuality in the genus be reassessed by testing more species from different clades. Different strategies to promote xenogamy in the Annonaceae are reviewed, including protogyny, herkogamy, intra- and inter-individual phenological synchrony and dioecy. Three different mechanisms were recognized to achieve dioecy, including incomplete pollen development in hermaphroditic flowers, delayed anther dehiscence in hermaphroditic flowers and loss of androecium or gynoecium (with Pseuduvaria used as a paradigm).
published_or_final_version
Biological Sciences
Doctoral
Doctor of Philosophy

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40

Takahashi, Daiki. "Evolutionary history and mechanisms for generating floral morphological diversity of Asarum (Aristolochiaceae) in East Asia." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263724.

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京都大学
新制・課程博士
博士(人間・環境学)
甲第23263号
人博第978号
京都大学大学院人間・環境学研究科相関環境学専攻
(主査)教授瀬戸口浩彰, 教授加藤眞, 教授市岡孝朗
学位規則第4条第1項該当
Doctor of Human and Environmental Studies
Kyoto University
DGAM

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41

Bukhari, Ghadeer, and Wenheng Zhang. "INDEPENDENT ORIGINATION OF FLORAL ZYGOMORPHY, A PREDICTED ADAPTIVE RESPONSE TO POLLINATORS: DEVELOPMENTAL AND GENETIC MECHANISMS." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4482.

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Observations of floral development indicate that floral organ initiation in pentapetalous flowers more commonly results in a medially positioned abaxial petal (MAB) than in a medially positioned adaxial petal (MAD), where the medial plane is defined by the stem and the bract during early floral development. It was proposed that the dominant MAB petal initiation might impose a developmental constraint that leads to the evolution of limited patterns of floral zygomorphy in Asteridae, a family in which the floral zygomorphy develops along the medial plane and results in a central ventral (CV) petal in mature flowers. Here, I investigate whether the pattern of floral organ initiation may limit patterns of floral zygomorphy to evolve in pentapetalous angiosperms. I analyzed floral diagrams representing 405 species in 330 genera of pentapetalous angiosperms to reconstruct the evolution of floral organ initiation and the evolution of developmental processes that give rise to floral zygomorphy on a phylogenetic framework. Results indicate that MAB petal initiation is the most common; it occupies 86.2% of diversity and represents the ancestral state of floral organ initiation in pentapetalous angiosperms. The MAD petal initiation evolved 28 times independently from the ancestral MAB petal initiation. Among the 34 independent originations of floral zygomorphy, 76.5% of these clades represent MAB petal initiation, among which only 47% of the clades result a CV petal in mature flowers. The discrepancy is explained by the existence of developmental processes that result in floral zygomorphy along oblique planes of floral symmetry in addition to along the medial plane. Findings suggest that although the early floral organ initiation plays a constraining role to the evolution of patterns of floral zygomorphy, the constraint diverges along phylogenetically distantly related groups that allow the independent originations of floral zygomorphy through distinct development processes in pentapetalous angiosperms. In additional study, the butterfly-like flowers of Schizanthus are adapted to pollination by bees, hummingbirds, and moths. I investigated the genetic basis of the zygomorphic corolla, for which development is key to the explosive pollen release mechanism found in the species of Schizanthus adapted to bee pollinators. I examined differential gene expression profiles across the zygomorphic corolla of Schizanthus pinnatus, a bee-pollinated species, by analyzing RNA transcriptome sequencing (RNA- seq). Data indicated that CYC2 is not expressed in the zygomorphic corolla of Sc. pinnatus, suggesting CYC2 is not involved in the development of floral zygomorphy in Schizanthus (Solanaceae). The data also indicated that a number of genes are differentially expressed across the corolla.

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Wood, Troy E. "Plant speciation (I) Species delimitation and pollinator driven floral evolution in the Giliopsis group of Ipomopsis (Polemoniaceae). (II) Polyploidy and vascular plant diversity /." [Bloomington, Ind.] : Indiana University, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3354925.

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Thesis (Ph.D.)--Indiana University, Dept. of Biology, 2009.
Title from PDF t.p. (viewed on Feb. 5, 2010). Source: Dissertation Abstracts International, Volume: 70-04, Section: B, page: 2009. Adviser: Loren H. Rieseberg.

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Bezerra, AntÃnio Diego de Melo. "Uso da abelha canudo (Scaptotrigona sp. nov.) na polinizaÃÃo do meloeiro (Cucumis melo L.) em ambiente protegido." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13832.

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Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico
As abelhas sÃo os principais agentes de polinizaÃÃo em culturas agrÃcolas. No caso do melÃo a falta desses agentes pode resultar uma queda na produÃÃo superior a 90%. Em cultivos protegidos a introduÃÃo de abelhas vem substituindo a mÃo de obra utilizada na polinizaÃÃo manual. O objetivo desse estudo foi investigar a viabilidade da utilizaÃÃo da abelha canudo (Scaptotrigona sp. nov.) na polinizaÃÃo do melÃo var. Natal, em ambiente protegido. Para tanto, a biologia floral do meloeiro, os requerimentos de polinizaÃÃo da cultura, o comportamento, forrageio e a eficiÃncia das abelhas Scaptotrigona sp. nov. na polinizaÃÃo no ambiente protegido. O experimento foi conduzido em casa de vegetaÃÃo localizada na Embrapa AgroindÃstria Tropical, no municÃpio de Fortaleza - CE. A variedade estudada Ã andromonoica, com antese das flores entre 6h00 e 6h30 e fechamento por volta 20h. Durante o perÃodo de floraÃÃo do meloeiro, as flores masculinas abrem primeiro e por volta do sÃtimo dia inicia a floraÃÃo da forma hermafrodita. ApÃs esse perÃodo ambas as formas florais se mantÃm constantemente sobrepostas atÃ o final do perÃodo de floraÃÃo, onde hÃ uma reduÃÃo das flores masculinas e na sequencia das femininas. A morfometria mostrou que as flores hermafroditas (fh) sÃo significativamente maiores no seu diÃmetro do que as flores masculinas (fm) (fh = 44,17 Â 5,77; fm= 39,29 Â 2,38 , p < 0,001). TambÃm foi observado que as flores hermafroditas produziram maior volume mÃdio de nÃctar (5,7 μL Â 2,72 μL) do que as flores masculinas (1,85 μL Â 0,47 μL) (p < 0,05). Quanto ao requerimento de polinizaÃÃo, as flores do meloeiro var. Natal apresentaram maiores taxas de vingamento de frutos por meio da polinizaÃÃo cruzada, diferindo significativamente do tratamento com polinizaÃÃo restrita (p < 0,05). Com relaÃÃo Ã avaliaÃÃo de Scaptotrigona sp. nov. no cultivo protegido, as operÃrias de foram capazes de visitar em poucas horas as flores da cultura no primeiro dia da abertura da colÃnia, mas as visitas aumentaram de forma efetiva a partir do quarto dia. Foi observado que as operÃrias buscaram tanto o pÃlen quanto o nÃctar nas duas formas florais e o nÃmero mÃdio de operÃrias que saiam para as atividades de forrageamento esteve correlacionado positivamente com o volume de nÃctar disponibilizado pelas flores hermafroditas (p < 0,05). Nesse estudo foi descrito pela primeira vez o comportamento de forrageio de Scaptotrigona sp. nov em cultivo protegido, mostrando a sua alta adaptabilidade e eficiente na polinizaÃÃo das flores do meloeiro var. Natal. AlÃm disso, o vingamento dos frutos por meio da polinizaÃÃo feita por essa abelha nÃo diferiu do tratamento com polinizaÃÃo manual cruzada. Portanto, recomendamos o uso de Scaptotrigona sp. nov. em cultivo protegido para a polinizaÃÃo do meloeiro, diminuindo assim os custos de polinizaÃÃo manual nessa cultura.
Bees are the main pollinators in agricultural crops. In the case of melon lack of these agents may result in a decrease in production over 90%. In crops protected the introduction of bees is replacing the manpower used in manual pollination. The aim of this study was to investigate the feasibility of using the straw bee (Scaptotrigona sp. new) At the pollination of melon var. Natal in a protected environment. Therefore, the melon floral biology, applications pollination of culture, behavior, foraging and efficiency of bees Scaptotrigona sp. new pollination in the greenhouse. The experiment was conducted in a greenhouse located in postharvest, in Fortaleza - CE. The variety is studied andromonoecious with anthesis of flowers between 6:00 am and 6:30 am and closing at around 20:00 h. During the melon flowering period, the male flower open first and by the seventh day starts flowering hermaphrodite form. After this period both floral shapes remains constantly overlapped by the end of the flowering period, where there is a reduction of the male flowers and the sequence of the female. Morphometry showed that the hermaphrodite flowers (hf) are significantly larger in diameter than the male flowers (mf) (hf = 44.17 Â 5.77, 39.29 Â mf = 2.38, p <0.001). It was also observed that the hermaphrodite flowers produced more nectar volume (5.7 Â 2.72 uL) than the male flowers (1.85 Â 0.47 uL) (p <0.05). As for the pollination of application, the melon flowers var. Natal had higher fruit set rates through cross-pollination, significantly different from the treatment with restricted pollination (p <0.05). As to the evaluation of Scaptotrigona sp. new in greenhouse of the workers were able to visit in a few hours the culture of flowers in the opening day of the colony, but the visits increased effectively from the fourth day. It was observed that the workers sought both the pollen and the nectar in two floral forms and the average number of workers leaving for foraging activity was positively correlated with the amount of nectar available by hermaphrodite flowers (p <0.05). In this study was first described the foraging behavior of Scaptotrigona sp. new protected cultivation, showing its high adaptability and efficient in flower pollination of melon var. Christmas. In addition, the ripening of fruits by means of pollination bees by this treatment did not differ from manual cross pollination. Therefore, we recommend the use of Scaptotrigona sp. Newin protected cultivation for pollination of melon; thereby reducing the manual pollination costs that culture.

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Santos, Mar?lia Grazielly Mendes dos. "O papel dos fatores abi?ticos e bi?ticos e dos atributos morfofuncionais na fenodin?mica de Himatanthus bracteatus (A. DC.) Woodson e Himatanthus drasticus (Mart.) Plumel (Apocynaceae) na floresta ciliar do Rio Len?ois, Chapada Diamantina, Bahia." Universidade Estadual de Feira de Santana, 2016. http://localhost:8080/tede/handle/tede/415.

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Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES
Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEB
This research addresses a study of methods of collection and phenological data analysisofHimatanthus bracteatus(A. DC.) Woodson e H. drasticus (Mart.) Plumel(Apocynaceae), and investigate their phenological patterns, relating them to the morphological and functional characters, biotic and abiotic factors. Monthly observations of leaf and reproductive phenology, and herbivory activity of these species were performed. We analyzed the leaf traits, water potential, chlorophyll and wood density (in dry months and rainymonths), and floral biology and floral visitor?s aspects. The normality of the data was tested by setting the corresponding regression and correlation tests. The morphological and functional characters were subjected to analysis of variance, considering each month as a treatment. In this study the quantitative method and the circular analysis were identified as the most appropriate for the intensity analysis and phenological timing of the species. The species were evergreen, with annual flowering and extend fruiting, overlaying all phenophases. Synchrony was note between reproductive phenophases. We identified the Pseudosphinx tetrio as a leaf predator. The precipitation was the trigger for the development of these caterpillars, observed in the host in rainy months. The morphofunctional characters showed water reserves in the stem of both species. The herbivorous activity had relationship with mature leaves and budding.The flowering became more intense in the absence of caterpillars. Thefloral morphology and biologyidentified phalenophily as the pollination syndrome. The aggregation and phenological patterns seems to be related to herbivory and pollination processes ofH. bracteatus and H.drasticus.
Essa pesquisa aborda um estudo de m?todos de coleta e an?lise de dados fenol?gicos de Himatanthus bracteatus(A. DC.) Woodson e H. drasticus(Mart.) Plumel (Apocynaceae), e investiga os seus padr?es fenol?gicos, relacionando-os com os caracteres morfofuncionais, fatores bi?ticos e abi?ticos. Para tanto, foram realizadas observa??es mensais da fenologia foliar e reprodutiva, e da atividade herb?vora dessas esp?cies. Analisamosos atributos foliares, potencial h?drico, clorofila e densidade da madeira (em meses de seca e chuva, durante o per?odo de estudo), aspectos de biologia floral e visitantes florais.A normalidade dos dados foi testada, definindo os testes de regress?o e correla??o correspondentes. Os caracteres morfofuncionais foram submetidos ?an?lise de vari?ncia, considerando cada m?s como um tratamento.Neste estudo o m?todo quantitativo e a an?lise circular foram identificados como os mais adequados para a an?lise de intensidade e sincronia fenol?gica das esp?cies. Em ambas as esp?cies, constatou-se padr?o perenif?lio,flora??o e frutifica??o anuais e longas, com sobreposi??o em todas as fenofases e forte sincronia entre as fenofases reprodutivas. Identificou-se a Pseudosphinx tetriocomo predadora, sendo a precipita??o o gatilho para o desenvolvimento destas, as quais foram observadas nos hospedeiros em meses chuvosos. Os caracteres morfofuncionais apontaram reserva h?drica no caule de ambas as esp?cies. A atividade herb?vora teve ainda rela??o com folhas maduras e brotamento.Aflora??o foi mais intensa na aus?ncia das lagartas.A morfologia e biologia floral apontam para a fanelofilia como s?ndrome de poliniza??o das esp?cies. A agrega??o e padr?esfenol?gicosparecem estar relacionados com a herbivoria e s?ndrome de poliniza??o em H. bracteatus e H.drasticus.

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45

Williams, Steven Keith. "Characterization of sterile tassel silky earl: A Homeotic B-Class Gene Involved in Specification of Floral Organ Identity In Zea mays." BYU ScholarsArchive, 2012. https://scholarsarchive.byu.edu/etd/3938.

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Specification of floral organ identity in angiosperm flowers is accomplished by the coordinated activity of A-, B-, C-, and E-class MADS-box genes. In the eudicots, B-class genes specify petal and stamen identity. This eudicot B-class function depends on the simultaneous expression of genes from two paralogous B-class lineages (the DEFICIENS/APETALA3 lineage and the GLOBOSA/PISTILLATA lineage). Proteins produced by genes from these two lineages interact as obligate heterodimers and together regulate the transcription of various downstream targets. These obligate heterodimers also positively regulate the transcription of the B-class genes themselves, thereby mediating a unique B-class autoregulatory feedback loop. There is compelling evidence that B-class function at the phenotypic and molecular level is highly conserved among the eudicots. The degree to which B-class homeotic function, obligate heterodimerization, and autoregulation are conserved in non-eudicot, however, remains a topic of debate. Here we describe loss of function in Sterile tassel silky ear1 (Sts1) a maize ortholog of GLOBOSA/PISTILLATA formerly known as Zmm16. Mutation in Sts1 results in homeotic transformation of lodicules and stamens into bract-like organs in male inflorescences. Female inflorescences are affected in a similar manner. Stamens in these inflorescences are, however, transformed into carpels instead of into bract-like organs. This mutant phenotype suggests that Sts1 has a B-class homeotic function. Using qRT-PCR we also demonstrate that Sts1 participates in positive transcriptional regulation of all of the maize B-class genes. These findings suggest a high degree of B-class functional conservation between the monocots and the eudicots. Analysis of tasselseed1/sts1 and grassy tillers1/sts1 double mutants suggests that maize B-class genes also play a role in the sex determination process.

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46

Barker, Daniel A. "Congruence and Temporal Variation of Floral Visitation and Pollen Transport Networks in Southern Appalachia." Digital Commons @ East Tennessee State University, 2008. https://dc.etsu.edu/etd/3811.

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Observation of floral visitation is an accepted method to describe plant-pollinator interactions despite potential biases. Collecting pollen from pollinators offers new insights on the structure and function of plant-pollinator communities. Furthermore, the strength and frequency of plant-pollinator interactions can vary across temporal scales. However, within-season and within-day (morning vs. evening) variation in plant-pollinator networks has been little studied. By evaluating variation in network structure across these biologically relevant time scales, we will gain a better understanding of the factors that shape plant-pollinator communities. The objectives of this study are to 1) Compare the structure of plant-pollinator networks built on floral visitation and pollen transport data, 2) Evaluate intra-annual variation in plant-pollinator network structure and 3) Evaluate variation in plant-pollinator structure within a single day (i.e. morning vs. evening). Congruence and Temporal Variation of Floral Visitation and Pollen Transport Networks in Southern Appalachia

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Barker, Daniel A. "Congruence and Temporal Variation of Floral Visitation and Pollen Transport Networks in Southern Appalachia." Digital Commons @ East Tennessee State University, 2020. https://dc.etsu.edu/etd/3811.

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Observation of floral visitation is an accepted method to describe plant-pollinator interactions despite potential biases. Collecting pollen from pollinators offers new insights on the structure and function of plant-pollinator communities. Furthermore, the strength and frequency of plant-pollinator interactions can vary across temporal scales. However, within-season and within-day (morning vs. evening) variation in plant-pollinator networks has been little studied. By evaluating variation in network structure across these biologically relevant time scales, we will gain a better understanding of the factors that shape plant-pollinator communities. The objectives of this study are to 1) Compare the structure of plant-pollinator networks built on floral visitation and pollen transport data, 2) Evaluate intra-annual variation in plant-pollinator network structure and 3) Evaluate variation in plant-pollinator structure within a single day (i.e. morning vs. evening). Congruence and Temporal Variation of Floral Visitation and Pollen Transport Networks in Southern Appalachia

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48

Chu, Yi-Hsuan. "The role of LC and FAS in regulating floral meristem and fruit locule number in tomato." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1512046877370248.

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Barker, Daniel A. "Congruence and within-season variation in floral visitation and pollen transport networks in Southern Appalachia plant-pollinator communities." Digital Commons @ East Tennessee State University, 2019. https://dc.etsu.edu/asrf/2019/schedule/227.

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Previous studies of plant-pollinator interactions have relied on the use of floral visitation data. Although, this may be insufficient to fully characterize the diversity and strength of plant-pollinator interactions. By using pollen transport data (i.e. pollen on pollinators), new insights can be gained on the structure and function of plant-pollinator communities. Yet studies that characterize and compare pollen-transport with floral-visitation networks are scarce. Furthermore, the strength and frequency of plant-pollinator interactions can vary across temporal scales. Although, monthly and within-day variation in network structure has been little studied. By evaluating variation in network structure across these biologically relevant time scales we will gain a better understanding of the factors that shape plant-pollinator communities. Here, we build plant-pollinator interactions networks based on floral visitation and pollen transport data by observing, collecting and sampling pollen from floral visitors in a southern Appalachian floral community. We aim to 1) compare the congruence of plant-pollinator networks built on floral visitation and pollen transport data and 2) evaluate within season and within-day variation in plant-pollinator network structure. To assess floral visitation and to quantify pollen transport, four 1x40m transects were set up at the study site. Morning collections were conducted between 8:00 AM and 3:00 PM twice per week while afternoon collections took place once per week between 3:01 PM and 5:00 PM over 20 non-consecutive days. All flower visitors observed interacting with a flower’s reproductive structures (i.e. searching for pollen and nectar) were collected.All collected pollinators were processed for surface pollen loads by dabbing the body with a 3x3mm fuschin jelly cube. Each area of the body was dabbed three times to standardize sampling. Identification and quantification of pollen was done using a compound light microscope. Data was then analyzed with the “bipartite” package of R to create bipartite plant-pollinator networks. Procrustes analysis was used to identify differences in network structure. Preliminary results show that the structure of floral visitation and pollen transport networks are significantly different from each other (P <0.01). Pollen-transport network size is almost four times larger (496 links) compared to the floral-visitation network (109 links). Species in the pollen transport network tend to be more connected (connectance = 2.3) and have five times more links per species on average (5.22 links) than floral visitation networks (connectance = 1.1, links = 1.8). Within-season and within-day differences in network structure are currently being evaluated. Our results so far show that pollen transport networks at our study site captured 78% more unique interactions and, thus, provide more accurate network structure. Interpretation of pollen transfer versus floral visitation networks can have important implications for our understanding of community-level functions such as their resilience and stability.

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Maass, Renê Marcos. "Interações ecológicas entre Poiretia latifolia Vogel (Fabaceae) e comunidades de insetos em povoamentos na região do Planalto Catarinense." Universidade do Estado de Santa Catarina, 2017. http://tede.udesc.br/handle/handle/2411.

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Capes
Poiretia latifolia Vogel (Fabaceae) is a promising native herb of the altitude field ecosystem, inserted in the Atlantic Forest biome in the southern region of Brazil. Its value lies in the composition of its essential oil that is rich in monoterpenes with potential for pharmaceutical and cosmetic use. The objective of this work was to study the insect fauna associated to P. latifolia, as pollinators, potential pests and natural enemies, as well as to evaluate the influence of these insects on the reproductive success of the plant. The insect fauna survey was carried out between December 2015 and January 2016, in two stands of P. latifolia located in the municipalities of Capão Alto and São José do Cerrito. The associated insect fauna was monitored for 42 hours in each area, using the method of Instantaneous Sweeping, being marked five plants in each area that were followed hourly from 9:00 a.m. to 4:00 p.m. It was evaluated the number of visiting insects and their interactions with the erva-de-touro, also using the analysis of the fluctuation of the three predominant orders. The influence of the meteorological variables was analyzed by the Pearson correlation coefficient. The behavior of the floral visiting insects was studied during 35 hours of observation in each area, using the method ad libitum, being evaluated the type of floral resource explored and the time of visitation. Specimens of each probable species sighted were collected and classified to the lowest taxonomic level possible. To determine herbivory damage, ten P. latifolia plants were marked in each area and flower and seed collections were performed to evaluate insect damage. Coleoptera, Diptera and Hymenoptera were the most sighted orders associated with erva-de-touro. Coleoptera were responsible for high damage to P. latifolia seeds, being that in Capão Alto 65% of the seeds were damaged by Apion sp., and in São José do Cerrito 87.6%. The damage to the flowers was also high, respectively 38 and 54% for the two evaluated areas. There was difference in the richness of floral visitors between stands. Hymenoptera were the main floral visitors, with Apis mellifera being the dominant species in both evaluated areas, but with greater dominance in São José do Cerrito. However, its behavior was purely as a nectar pillager, as were all other species recorded. Environmental changes caused by the intensity of agricultural use close to the stands of Poiretia latifolia may have caused these differences
A erva-de-touro Poiretia latifolia Vogel (Fabaceae) é uma promissora erva nativa do ecossistema campos de altitude, inserido no bioma Mata Atlântica na região Sul do Brasil. Seu valor se encontra na composição do seu óleo essencial que é rico em monoterpenos com potencial de uso farmacêutico e cosmético. O objetivo deste trabalho foi estudar a entomofauna associada a P. latifolia, como agentes polinizadores, pragas potenciais e inimigos naturais, bem como avaliar a influência destes insetos no sucesso reprodutivo da planta. O levantamento da entomofauna foi realizado no período de dezembro de 2015 a janeiro de 2016, em dois povoamentos de P. latifolia localizados nos municípios de Capão Alto e São José do Cerrito. A entomofauna associada foi monitorada por 42 horas em cada área, utilizando-se o método de Varredura Instantânea, sendo marcadas cinco plantas em cada área que foram acompanhadas de hora em hora, das 9:00 às 16:00. Foi avaliado o número de insetos visitantes e suas interações com a erva-de-touro, utilizando-se também a análise da flutuação das três ordens predominantes. A influência das variáveis meteorológicas foi analisada pelo coeficiente de correlação de Pearson. O comportamento dos insetos visitantes florais foi estudado durante 35 horas de observação em cada área, utilizando-se o método ad libitum, sendo avaliado o tipo de recurso floral explorado e o tempo de visitação. Espécimes de cada provável espécie avistada foram coletados e classificados até o menor nível taxonômico possível. Para determinar os danos por herbivoria, foram marcadas dez plantas de P. latifolia em cada área e realizadas coletas de flores e sementes para avaliar o dano por insetos. Coleoptera, Diptera e Hymenoptera foram as ordens mais avistadas associadas a erva-de-touro. Diptera demonstrou correlação com a temperatura média diária. Os coleópteros foram responsáveis por elevados danos às sementes de P. latifolia, sendo que em Capão Alto 65% das sementes estavam danificadas por Apion sp., e em São José do Cerrito 87,6%. O dano às flores também foi elevado, respectivamente 38 e 54% para as duas áreas avaliadas. Houve diferença na riqueza de visitantes florais entre os povoamentos. Himenópteros foram os principais visitantes florais, sendo Apis mellifera a espécie dominante em ambas as áreas avaliadas, porém com maior dominância em São José do Cerrito. Entretanto seu comportamento foi meramente como pilhadora de néctar, assim como todas as demais espécies registradas. Alterações ambientais causadas pela intensidade de uso agrícola próximo aos povoamentos de Poiretia latifolia podem ter causado estas diferenças

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Dissertations / Theses on the topic 'Floral biology'

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