Dissertations / Theses: 'Fields of Research – 300000 Agricultural, Veterinary and Environmental Sciences – 300400 Animal Production'

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Muir, Paul David. "Studies on the growth and compositional development of antlers in red deer (Cervus elaphus)." Lincoln College, University of Canterbury, 1985. http://hdl.handle.net/10182/1178.

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The experiments described in this thesis investigated nutritional and physiological aspects of antler growth in red deer stags. The initial experiment (Section 3) examined the effects of winter nutrition on subsequent antler casting date and velvet antler weight. Mature stags on two different farm types (hill country, Farm H and irrigated lowland, Farm L) were offered three levels of winter nutrition, two levels of a concentrate supplement (ad libitum pellets and 1/2 ad libitum pellets) and a basal hay ration. On both properties liveweight gains occurred in supplemented groups and liveweight losses in unsupplemented groups. At antler casting there were significant differences in liveweight of approximately 10 kg between fully supplemented and unsupplemented groups. Realimentation of winter liveweight losses subsequently occurred so that by the following rut the effects of winter undernutrition had been eliminated. On Farm H poor winter nutrition (hay only) resulted in a significant delay in casting date (13 days) and lower velvet antler yields (0.24 kg), than in stags offered the ad libitum concentrate ration. Stags on Farm H were 13 kg lighter at commencement of the trial than at Farm L and the differences in treatment effects obtained between farms may have been due to differences in body condition at commencement of the trial. An association was demonstrated between liveweight and date of antler casting, with heavier stags casting earlier than lighter stags. There was no effect of age of stag on casting date. Of the liveweights recorded, liveweight prior to the rut showed the best relationship with casting date, possibly because the seasonal nature of liveweight change meant that a weight recorded at this time gave the best indicator of the true frame size of a stag. Both age and liveweight significantly affected velvet antler weight, with increases of velvet antler weight of 0.26 kg between 3 and 4-year-old stags and of 0.30 kg between 4 and 5-year-old stags at the same liveweight. Within an age group velvet weight increased by 0.12 kg for each 10 kg increase in pre-rut liveweight. The experiments described in Section 4 comprised studies on antler growth and composition. In order to obtain data on antler growth and composition individual antlers were removed sequentially from mature red deer stags between 28 and 112 days after casting of hard antlers. Contralateral antlers were removed after stripping of velvet. Wide variation occurred in antler casting date (53 days) compared to date of velvet stripping (24 days). The duration of the period of antler growth may therefore be governed more by date of casting than by date of velvet stripping. Mean duration of the antler growth period was 164 days. Growth in length of the antler appeared to follow a sigmoid curve. However, between 28 and 112 days after casting, rates of elongation were close to linear. Mean length of hard stripped antlers was 0.71 m and between 28 to 112 days after casting mean rate of antler elongation was 0.62 cm/day. Over this period indivdual antlers increased in fresh weight at a rate of 13.7 g/d, with heaviest weight recorded 112 days after antler casting, at approximately 130% of final hard antler weight. Between 28 and 91 days of growth, volume of blood in the antler increased linearly at a rate of 194 ml/kg. Three phases of mineralization were demonstrated in developing antlers. Tips of growing antlers were cartilaginous and poorly mineralized. A zone of mineralization occurred 5.0 to 7.5 cm behind the antler tip which corresponded histologically to the transition from mineralized cartilage to trabecular bone. The second phase of mineralization occurred through continued accretion of trabecular bone in the antler shaft. The third phase, described as "terminal mineralization" in this study, appeared to be associated with a rapid increase in density of cortical bone in the periphery of the antler shaft. Terminal mineralization (between 91 and 112 days after casting of hard antlers) coincided with the slowing of growth in length, a decrease in relative blood volume in the antler and an increase in levels of plasma testosterone. These events occurred close to the summer solstice. At velvet stripping individual antlers had a mean weight of 1.12 kg and contained 81.1% dry matter (DM). Fat free organic matter (FFOM) and ash concentration in DM were 36.6 and 60.0%, respectively. Peak daily rates of FFOM and ash deposition occurred between 91 days and 112 days after casting, at rates of 1.4% of hard antler FFOM and 1.6% of hard antler ash. For a stag producing 2.24 kg of hard antler mean rates of FFOM and ash deposition over this period were 9.3 and 18.3 g/d, respectively. On a whole antler basis calcium concentration in antler ash remained constant, at around 35%. Therefore peak rate of antler calcium deposition would be 6.4 g/d. In the final experiment (Section 5) mature stags were offered a maintenance ration of greenfeed oats during the period of peak calcium requirement for antler growth and the kinetics of calcium metabolism were examined using a radio-isotope (⁴⁵Ca). Rates of faecal endogenous loss were low and at approximately 6.4 mg/kg BW per were half the estimated requirements of ARC (1980) for sheep and cattle. Availability of calcium from greenfeed oats was low (mean, 37%) and less than 30% of total calcium requirements were derived from the diet. Poorly mineralized skeletal bones indicated that the shortfall in antler calcium was derived from the skeleton. In spite of a severely negative calcium balance stags were capable of maintaining high and apparently normal rates of antler calcium deposition (mean, 44 mg/kg BW per day). Antlers appear to be acting as a sink with calcium being irreversibly deposited in the antler and lost to the animal's body. On the assumption therefore that antler calcium behaves like calcium lost during lactation a kinetic model of calcium metabolism in the stag was developed.

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Lin, Xiaoxiao. "Evaluation of Kahne rumen sensors in fistulated sheep and cattle under contrasting feeding conditions : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Agriculture at Massey University, Palmerston North, New Zealand." Massey University, 2009. http://hdl.handle.net/10179/1313.

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The Kahne rumen sensor (bolus) is a device developed to measure temperature, pressure and pH in non-fistulated animals. This bolus allows real-time monitoring of the rumen environment, which could help preventing health problems such as rumen acidosis in cows. It is less invasive to use boluses compared to other technologies that measure the ruminal pH (e.g. rumenocentesis). Kahne boluses and transceivers are commercially available in the market. Several studies on the relationships between data recorded by the bolus and actual data recorded by independent devices were conducted. The bolus temperature and pressure were compared with actual temperature and pressure under controlled conditions. The pH drift was studied by comparing the difference between bolus and direct measurement over time. The capture of the data was calculated for each bolus in various experiments to examine the factors affecting the data capture rate of the boluses. Animal to animal variation was studied using boluses in a group of cows fed and managed under uniform conditions. An animal experiment involving fistulated cows eating two different diets was performed using boluses to monitor the changes of ruminal pH. There was no apparent interruption to normal animal behaviour as a result of using boluses. Regression relationships between bolus measurements and actual data for both temperature and pressure were developed and used for calibration of bolus data. The pH drift was a problem, as the regression relation between the pH difference and the time for one bolus from one experiment could not represent this bolus on other experiment. The data capture rate on the hourly basis ranged from 0 to 100%, but was usually between 30 to 70%. The data capture rate was affected by many factors and further studies to identify these factors are needed. A study of animal to animal variation suggests that in a comparison of 2 treatments, a minimum 3 cows per group would be required to detect the standard deviation of 0.11 for a pH difference of 5% of the mean (approximately 0.35 pH units). Seventeen cows per group would be required to detect the standard deviation of 0.33 for the same difference. The boluses effectively monitored the ruminal pH change in cows easting two different diets and the profile of change of pH was successfully analysed. Feeding 7.6 kg baleage twice a day cause pH to decrease at 0.009 pH units per minute during feeding, while offering a similar quantity of grass and hay once a day resulted in a decrease of 0.0009 pH units per minute during feeding. The beginning of pH increase was about 1 hour following feeding and continuous during resting and rumination. The level of pH increase did not differ significantly for two diets.. The Kahne devices appear to have advantages compared to other technologies for the measurement of parameters of the rumen environment on a real-time basis. Boluses are especially good at intensively monitoring the temperature, pressure, and pH in the rumen. The major limitations of this technology to be used are the data capture rate and the pH drift. By improving the limitations found in the experiment, the Kahne rumen sensor could become very useful for both scientific research and under commercial conditions for monitoring animal health.

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Bates, Andrew John. "Effects of grazing management and pasture composition on the nitrogen dynamics of a dairy farm : a simulation analysis : a thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy at Lincoln University /." Diss., Lincoln University, 2009. http://hdl.handle.net/10182/1360.

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There is an extensive debate on the potential environmental impact of dairy farms and in particular the effect of dairy farms on the nitrogen cycle and the effect that this has on ecosystems. Within New Zealand and in particular in the South Island, the expansion of dairying and the adoption of new dairy systems has led to this becoming an increasingly important issue, locally through its effect on water quality and the environment and nationally and internationally through the production of green house gases. Increases in nitrogen usage at the expense of clover nitrogen fixation, irrigation, stocking rate and the introduction of dairy cows onto light free draining soils previously the preserve of arable or sheep farming has led to concern as to the effect intensive pastoral dairying may have on the nitrogen dynamics of the farm and the environment. This study is designed to assess how changes in grazing management in particular changes in pre-grazing and post-grazing residuals alter the clover/ryegrass balance on the farm and the effect that this has on the farm’s nitrogen dynamics. The effects of qualitative changes in grazing management on pasture composition are well established but little is known of the effect of quantitative changes in pasture management on composition, in particular the effect of grazing residuals. There are a number of detailed models of the physiological processes in the energy and nutrient cycling in plants, animals and the soil. There are a smaller number of whole farm models that through integration and simplification of component models attempt to represent the flux of nutrients though a dairy farm. None of these whole farm models is currently able to model the nitrogen flux through a dairy farm at a sufficient level of resolution to capture differences in pasture composition as these occur spatially, temporally and in response to grazing management. This project sought to better understand the nitrogen dynamics on a dairy farm by constructing and then linking component models – a pasture composition and growth model, a cow model, an excretal return model, a soil model and a water balance model – within a whole farm management schedule. The formal null hypothesis is that the mechanistic, mathematical model constructed for this PhD cannot capture and explain the full range of the changes in soil water content, soil nitrogen status, pasture production and composition and animal production, following the alteration in management of the dairy farm between 2002 and 2004. Individual component models were constructed by the author using the computer software package (Matlab) and validated against data extracted from the literature. The models were then converted into one simulation package using C-sharp as the source code language by Elizabeth Post, Senior Computer Scientist at Lincoln Ventures Ltd, Lincoln, New Zealand and the author. This model was then used to investigate the nitrogen dynamics of a dairy farm: the relationship with pasture composition and whether small changes in pasture residuals make a difference to pasture composition and nitrogen dynamics. Two different simulations were run based on the management practice of Lincoln University Dairy farm (LUDF) over two dairy seasons (2002-03 and 2003-04) and validated against the data recorded on this farm. In 2002-03, 50 cows were over wintered and 580 cows were subsequently milked on 200ha. Post grazing residuals where maintained at 1600-1750KgDM/ha. In 2003-04, 125 cows were overwintered and 635 cows were milked on 200ha with post grazing residuals maintained at 1400KgDm/ha. All models operate on a daily time step. Within the pasture model composition is described by 9 state variables describing different components of the pasture and pasture growth is modelled mechanistically from a calculation of component photosynthesis. A further 9 state variables describe the nitrogen composition of the pasture components. The soil model is a variable two layer, mechanistic representation, parametised for the shallow, stony soils of LUDF. Soil water status is an input for the pasture model while water uptake by the growing plants affects the soil water balance within the soil model. Animal intake and production are modelled mechanistically with model cows described in terms of their age, genetic merit, body weight, breed, pregnancy status, conception date and body condition score. Each cow type produces a different quantity of urinary and faecal excretion which varies with dry matter intake, milk yield and the sodium and potassium status of the pasture. Excretal nitrogen composition is predicted within a separate model which calculates daily nitrogen excretion in faeces, urine and milk. Excretions are deposited randomly over the grazed area and account is taken of overlapping excretions that are created on the same day and overlaps that occur with older excretal patches deposited in previous grazing rounds. Each excretal patch has its own associated pasture, water and soil model reflecting the differences in nitrogen status between patches. Grazing preference is expressed within the model between different classes of excretal patch and between excretal patches and the base pasture and between clover and grass. Supplementary silage is conserved and fed according to the management schedule of LUDF. Cows calve, become pregnant and are dried off within the model according to the relevant records from LUDF. Cows are deemed to arrive on the farm on the day of calving and to leave on the day that drying off is finished (a 5 day procedure within the model), except for those cows that are overwintering which remain on the farm. The soil model has multiple nitrogen/carbon pools and is dynamically linked to all the other models. External nitrogen losses from the system are modelled as volatilisation, leaching and denitrification, with pasture nitrogen uptake from the soil model and fixation by clover from the atmosphere. Both the individual component models and the final assembled composite model were successful in matching the available data in terms of pasture and animal production, pasture composition, soil water balance and nitrogen status and external losses. The model indicates that the low residual, high stocking rate farm returns more excreta to the soil. However, this is countered by a reduction in the amount of dead material returned to the paddock and this reduces the relative size of the pool of nitrogen in the dead organic matter. This produces a relative lack of substrate for the soil microbes which are thus unable to exploit all of the nitrogen in the available pool. Soil ammonium and nitrate pools are also increased from the increase in faecal and urinary return so precipitating an immobilising flux from these larger pools to the smaller pool of nitrogen available to the soil microbes. However, the relative inability of the soil bacteria to fully exploit this means that the production of soil organic live matter and the resulting mineralising flux from the dead organic matter pool through the available pool to the ammonium and nitrate pools is reduced. The larger ammonium and nitrate pools will also be associated with increased external losses from the system as denitrification, leaching and volatilisation are increased. The increase in the clover percentage within the sward in 2003-04 led to greater nitrogen fixation and the model suggests that some of the extra nitrogen is effectively captured by the animals in increased production. However, the reduction in the return of dead matter coupled with an increase in excretal return and the consequent increase in the mineral nitrogen pools within the soil lead to greater losses of nitrogen from the soil.

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Thomas, Craig W. "Current practices and future possibilities of performance recording extensively-grazed commercial beef herds in New Zealand." Diss., Lincoln University, 2008. http://hdl.handle.net/10182/844.

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There is little evidence that the productivity of New Zealand beef herds has improved over time. Data from the NZ Meat and Wool Board’s Economic Service (2006) suggest that the average national calving percentage has declined over the last two decades. During the same period cattle carcass weights have increased but so too has the average cow live-weight which has resulted in increased maintenance costs of the cow herds. It is unclear whether production efficiency in the industry has improved or declined over time. The aim of this research was to develop means of improving productivity in commercial beef herds through practical methods of performance recording. The objectives were firstly to establish current management practices in commercial herds and secondly to develop an objective system for cow selection and culling which would have practical application in commercial herds. Beef management survey Ninety two commercial beef producers with more than 100 breeding cows from the greater Canterbury region of New Zealand were surveyed. Pasture control was the main reason given for owning a beef herd. Size and conformation were the main selection criteria for choosing replacement heifers and bulls. Over 80% of herds retained their own heifers as replacements and >60% mated yearling heifers to first calve at two years of age. Fertility was poor in the surveyed herds. In-calf rates at pregnancy testing averaged 88% for maiden heifers, 92% for rising second calvers and 93% for mixed age (m.a.) cows. There was no significant difference between in-calf rates of maiden heifers mated to first calve at two or three years of age; nor was there any significant difference between the re-breeding success of the two groups. Heifers mated at least one week earlier than m.a. cows, achieved a re-breeding success 4.7% greater (P<.01) than those mated at the same time. Reasons for cows not weaning a calf included wet dry (9.3% of pregnant cows wintered), pregnancy tested not-in-calf (7.4%) and dam death (2.6%). Only 87.9% of pregnant females wintered weaned a calf (89.4% of m.a. cows and 84.9% of heifers). Reasons why cows exited the herds included diagnosed empty (37.2% of all exits), involuntary culls (25.4%), sold wet dry (16.2%), deaths (13.1%) and poor calf production (5.1%). Vaccination was infrequent with clostridial vaccines the most common in m.a. cows (15.2%) and in calves (40.7%); vaccination against Leptospirosis was much less common. Very few of the surveyed farms used any system of performance recording; as a result there was very little performance-based selection or culling practiced. Evaluation of alternative measures of cow productivity Data from four performance recording beef herds were used to compare alternative measures of cow productivity with the industry standard which is calf weaning weight adjusted for sex (SOC) and age of calf and age of dam (AOD), i.e. the “200 day weight.” None of the alternative measures evaluated required knowledge of calving date and all were relatively easily obtainable in extensively managed beef herds. The assessment of cows was based not on their estimated breeding values but instead on their most probable producing ability which, as the sum of all of the permanent, repeatable aspects of the calf-rearing ability of the cow, explains considerably more of the variance of weaning weight than does breeding value alone. SOC and AOD-adjusted marking weight, weaning weight and average daily gain (ADG) between marking and weaning were the traits mostly highly correlated with the 200d wt of calves (r = 0.68, 0.90 and 0.74. respectively). An Extensively- Grazed-Cow-Weaning-Index of these three indicator traits was found to be more highly correlated than any of the individual traits on their own (r = 0.94). Index weights for the three indicator traits were calculated within each herd and then those within-herd index weights were regressed on readily obtainable herd descriptive variables to obtain a regression equation that could predict index weights for any herd. When the model was applied to data from two additional herd years not included in the original model, the EGCW Index was highly correlated with the 200d weights (r>0.90). Performance-based culling of previously unselected commercial beef herds based on the EGCW Index will result in improved productivity due to the moderately high repeatability of calf weaning weight. Objective data from extensively grazed commercial herds will also make possible the use of commercial herd data in genetic evaluations of herd sires.

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Dobson-Hill, Brenna Catherine. "Uterine involution in the dairy cow : comparative study between organic and conventional dairy cows : a thesis presented in partial fulfilment of the requirements for degree of Masters of Science in Animal Science, Massey University, Palmerston North." Massey University, 2009. http://hdl.handle.net/10179/1272.

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Organic dairying is growing in popularity in New Zealand and the demand for knowledge is ever increasing. Many more farmers are considering organics as an alternative to their current conventional system. However, limited information is available on organic dairying in a New Zealand pastoral situation particularly in relation to its effect on fertility. The New Zealand dairy industry requires the dairy cow to become pregnant every year at the same time to maximise milk production and utilise the high pasture production in spring. A cow requires 40 to 60 days for uterine involution to occur and for resumption of oestrous cycles. It is critical that the cow becomes pregnant within the six to eight week mating period, however, this can be difficult for late calvers or those cows that had or still have a uterine infection, to undergo uterine involution and be successfully mated. These cows will often not become pregnant and as a consequence be culled for infertility. During the first few weeks postpartum, the bacterial composition of the uterus fluctuates as a result of spontaneous contamination, elimination and recontamination. Most cows are able to eliminate these bacteria, however, 10 to 17% of cows are unable to do this. In these cows, the bacteria persist, cause infection and inflammation, and delay uterine involution. The main objective of this present study was to investigate the effect of different management systems (Organic and Conventional) on productivity and reproduction, particularly the process of uterine involution and its relationship with reproductive outcomes. Uterine involution will be studied through the following measurements: cervical diameter as assessed by measurement per rectum, plasma concentrations of glucocorticoids and bacteriology of the uterus. Lactation characteristics: milk yield, cumulative milk yield, milksolids production and somatic cell count will also be investigated. From the results, it can be concluded that Organic cows have reduced milk yield (P=0.05) and milksolids production (P<0.01) through there were no major differences at peak lactation or in the rate of decline after. However, overall somatic cell count was not affected by management system though the Conventional cows had high somatic cell counts in early lactation (P=0.925). Cervical diameter was affected by the management system with the difference between Organic and Conventional cows almost significant (P=0.06). Cortisol concentrations were significantly higher in Organic cows (0.68 ± 0.08 ng/ml) when compared to Conventional cows (0.95 ± 0.06 ng/ml) (P=0.01). Cortisol concentrations decreased over time postpartum (P<0.01). Additionally Organic cows had a shorter interval from calving to AI (P=0.017). However, none of the other reproductive outcomes were affected by management system. Simple correlations established that reproductive outcomes and bacterial counts, reproductive outcomes and cortisol at Day seven, and cortisol and milk production characteristics, were not independent of each other. Aerobic bacterial counts on Day 28 and the interval from calving to AI were found to be significantly correlated (r= 0.615). Stepwise partial regression analysis, plus analysis of variance calculated on the relationships between uterine involution parameters and reproductive outcomes found multiple weak correlations. It found that cervical diameter, and aerobic and anaerobic bacterial counts on Day 28 were significantly correlated with the interval from calving to first AI (P=0.018). The differences in lactation characteristics are related to the lower pasture production on the organic farmlet reducing the maximal potential milk production and the possibly earlier calving dates of the Conventional cows. The differences in bacterial counts and almost significant difference in cervical diameter may be related to the lower cortisol levels and thus suggest that the Organic cows may be less stressed. As stress suppresses the immune system, it may be possible that the higher bacterial counts are indicitative of a less efficient or slightly weakened immune system which takes longer to eliminate bacteria in the uterus; however this is open to conjecture. The shorter interval from calving to AI suggests that Organic cows may be exhibiting oestrus behaviour earlier postpartum or the Conventional cows are having “silent heats” but this difference is possibly related to human factors. The correlation between parameters of uterine involution and interval from calving to AI, and the lower values for uterine involution parameters in Organic cows suggest the possibility of faster uterine involution and this may result in better fertility in the Organic cows. More research needs to be carried out to further investigate the effect of organic dairying on uterine involution and reproduction outcomes but organic dairying at the very least does not negatively impact on uterine involution and fertility.

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Linden, Danitsja Stephanie van der. "Ewe size and nutrition during pregnancy : effects on metabolic and productive performance of the offspring : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Turitea, Palmerston North, New Zealand." Massey University, 2010. http://hdl.handle.net/10179/1343.

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Exposure of the fetus to adverse conditions in utero may result in developmental adaptations that alter metabolism and postnatal growth of the offspring. This thesis investigated the effects of dam size and nutrition during pregnancy on growth, metabolic function and lactational and productive performance of the female offspring to two years of age. Four-hundred and fifty heavy (60.8 kg ± 0.18) and 450 light (42.5 kg ± 0.17) dams were randomly allocated to ad libitum or maintenance nutritional regimens from days 21 - 140 of pregnancy, under pastoral grazing conditions. From one week prior to lambing, all dams were fed ad libitum until weaning. After weaning, female progeny were managed and fed under pastoral conditions as one group. Maternal nutrition during pregnancy affected lamb growth to weaning, however, after weaning lamb growth was affected by dam size. Dam size had no effect on glucose metabolism, adrenal function or fat metabolism in 16-month-old female twin offspring. Dam nutrition during pregnancy had a minor effect on glucose metabolism and no effect on adrenal function or lipolysis, however, it did possibly affect gluconeogenesis and/or glycogenolysis, with increased glucose production in ewes born to maintenance-fed dams. Ewes born to dams fed maintenance showed greater milk production, lactose percentage, lactose and crude protein yield. Ewes born to heavy dams showed greater milk production and lactose yield. Dam size had no effect on reproductive performance of the female offspring. Being born to a larger dam showed no advantages over being born to smaller dams, for number of lambs born and weight of lambs at birth and weaning. ‘Grand’dam maintenance nutrition increased lamb birth and weaning weight and lamb growth rates of the ‘grand’offspring. Ewes born to maintenance-fed dams could have an advantage over ewes born to ad libitum-fed dams in physiological stressful situations in life as their liver may be able to supply more glucose to support their growing conceptus and milk production to increase the chances of survival of their offspring. These results indicate that it is possible to programme the offspring by feeding their dams differently during pregnancy under grazing conditions. With a better understanding of how offspring can be programmed through different maternal nutritional regimens, it may be possible to significantly increase the production potential of the New Zealand ewe population.

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Nalle, Catootjie Lusje. "Nutritional evaluation of grain legumes for poultry : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Poultry Nutrition at Massey University, Palmerston North, New Zealand." Massey University, 2009. http://hdl.handle.net/10179/1021.

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The nutritional value of faba beans (Vicia faba), Australian sweet lupins (Lupinus angustifolius), white lupins (Lupinus albus) and peas (Pisum sativum) grown in New Zealand for broilers were evaluated in terms of their nutritional characteristics, protein quality (protein efficiency ratio), apparent metabolisable energy, apparent ileal digestibility coefficient of amino acids and the effects on bird performance. The effects of dehulling and extrusion cooking on the nutritive value of legumes were also investigated. The first experiment discussed in Chapter 3 evaluated the effect of cultivars on the nutrient profile and protein quality of chickpeas (Cicer arietinum), Australian sweet lupins, peas and soybeans (Glycine max). With the exception of white lupins, cultivars had no effect on the proximate and fibre composition of grain legumes. Starch was the primary carbohydrate component of chickpeas and peas, whilst non-starch polysaccharides were the major carbohydrates in lupins. The legume proteins were deficient in lysine, methionine, cystine and threonine. No differences were found in protein quality between cultivars of the different grain legume species. The lowest weight gain and protein efficiency ratio, in addition to the highest relative pancreatic weight and mortality rate was found in raw soybeans, suggesting that soybeans contained high a concentration of anti-nutritional factors, such as protease inhibitors. Birds fed chickpeas, lupins and peas had a low mortality rate and relative pancreatic weight, confirming that the level of anti-nutrients in these legume seeds was low. The apparent metabolisable energy and apparent ileal digestibility coefficient of amino acids of faba beans, Australian sweet lupins, white lupins and peas were determined in the second experiment (Chapter 4). Cultivar effect on the apparent metabolisable energy values was observed only for faba beans and white lupins. Faba beans, white lupins and peas had comparable apparent metabolisable energy values, but these values were higher than those of Australian sweet lupins, and lower than that of soybean meal. No cultivar differences were found in the apparent ileal digestibility coefficient of amino acids of grain legumes. The apparent ileal digestibility coefficient of amino acids of both lupin species was found to be comparable to that of soybean meal. The effects of feeding diets containing 200 g/kg faba beans, lupins or peas on the performance, digestive tract development and litter quality of broilers were investigated in the third and fourth trials. In the cage trial (Chapter 5), the results showed that the weight gain of birds fed diets containing grain legumes was similar to that of control diet. Feed intake and feed per gain of birds fed diets containing the majority of grain legume cultivars did not differ from those fed the maize-soy diet. Birds fed diets containing faba beans had more dry and friable excreta compared to other treatment diets. The performance of birds fed diets containing 200 g/kg grain legumes during the 35 d grow-out period, in the floor pen trial (Chapter 6), confirmed the results of the cage trial. In this trial, weight gain and feed per gain of birds fed diets without meat meal were superior to those with meat meal. In cage trials, the modification of some segments of digestive tract development was probably due to the dietary NSP. Whilst in floor pen trial, digestive tract development was not influenced by the inclusion of grain legumes. The effect of methodology of determination (direct vs. difference method) on the apparent ileal digestibility coefficient of amino acids of wheat, maize, Australian sweet lupins, peas and soybean meal for broilers was evaluated in the fifth study (Chapter 7). The influence of methodology on apparent ileal digestibility coefficient of amino acids was found to vary amongst the feed ingredients. In general, the apparent ileal digestibility coefficient of amino acids of test ingredients determined by the difference method was higher than those determined by the direct method, suggesting that the use of the direct method may underestimate the apparent ileal digestibility coefficient of amino acids in low and medium protein ingredients. Data reported in Chapter 8 shows that dehulling increased the apparent metabolisable energy values of faba beans and Australian sweet lupins, but it had no beneficial effect on peas. The increase of apparent metabolisable energy values may be attributed to the decrease in non-starch polysaccharides of these legume seeds after dehulling. The removal of hulls increased the amino acid concentrations, but it had no effect on the apparent ileal digestibility coefficient of most amino acids. These results suggest that dehulling of grain legumes would be nutritionally beneficial and, likely to be economical in view of the improved amino acid concentrations and energy values. The final experiment (Chapter 9) demonstrated that extrusion of peas markedly influenced the content of crude protein, non-starch polysaccharides, starch, and trypsin inhibitors. The soluble non-starch polysaccharides and trypsin inhibitor contents of the majority of extruded pea samples were higher than those of raw peas, but insoluble and total non-starch polysaccharides decreased with extrusion. Extrusion had no effect on the apparent ileal protein digestibility and the apparent metabolisable energy of peas, but it increased ileal starch digestibility.

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Prattley, Deborah Jayne. "Risk-based suveillance in animal health : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Massey University, Palmerston North, New Zealand." Massey University, 2009. http://hdl.handle.net/10179/1011.

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Animal health surveillance is an important part of animal health care, particularly in countries dependent on livestock for food production and international trade. There are two major issues related to the provision of e®ective surveillance activities. Firstly, for good information to become available, the design and conduct of data collection activ- ities should be carried out following sound statistical principles. In reality, constraints such as imperfect tests and unavoidably-biased sampling strategies hinder straightfor- ward analysis and interpretation of survey results. Risk-based surveillance is used to target high-risk sub-populations to increase e±ciency of disease detection; however, biased datasets are generated. This thesis develops methodologies to design risk-based surveillance systems and al- low statistically valid analysis of the inherently biased data they generate. The ¯rst example describes the development of a method to analyse surveillance data gathered for bovine spongiform encephalopathy (BSE). The data are collected from four dif- ferent surveillance streams of animals tested for BSE, with each stream containing unavoidable biases and limitations. In the BSurvE model, these data are combined with demographic information for each birth cohort to estimate the proportion of each birth cohort infected with BSE. The prevalence of BSE in a national herd can then be estimated using the method of moments, whereby the observed number of infected animals is equated with the expected number. The upper 95% con¯dence limit for the prevalence is estimated both for infected countries and for those where no BSE has previously been detected. A similar approach to that used in BSurvE is then applied to surveillance data for trichinellosis, for which risk-based post-mortem testing is also performed. Negative results from multiple species using di®erent, imperfect tests are combined to give an estimate of the upper 95% con¯dence limit of the national prevalence of trichinellosis in a reference population. This method is used to provide support for freedom from trichinellosis in Great Britain. A di®erent approach to risk-based surveillance is explored as the surveillance strategy for detection of exotic causes of abortion in sheep and goats in New Zealand is examined. Using a geographic information system (GIS) maps of disease risk factors were overlain to produce a risk landscape for the lower North Island. This was used to demonstrate how areas of high- and low-risk of disease occurrence can be identi¯ed and used to guide the design of a risk-based surveillance programme. Secondly, within one surveillance objective there may be many ways in which the available funds or human resources could be distributed. This thesis develops a method to assess BSE surveillance programmes, and provides tools to facilitate BSE detection on the basis of infection risk and to increase the e±ciency of surveillance strategies. A novel approach to allocation of resources is developed, where portfolio theory con- cepts from ¯nance are applied to animal health surveillance. The example of surveil- lance for exotic causes of sheep and goat abortion is expanded upon. Risk of disease occurrence is assessed for a population over di®erent time periods and geographical areas within a country, and portfolio theory used to allocate the number of tests to be carried out within each of these boundaries. This method is shown to be more likely to detect disease in a population when compared to proportional allocation of the available resources. The studies presented here show new approaches that allow better utilisation of imperfect data and more e±cient use of available resources. They allow development of surveillance programmes containing an appropriate balance of scanning and targeted surveillance activities. Application of these methods will enhance the implementation and value of surveillance in animal health.

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9

Benschop, Jacqueline. "Epidemiological investigations of surveillance strategies of zoonotic Salmonella : a dissertation presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy at Massey University." Massey University, 2009. http://hdl.handle.net/10179/1025.

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This thesis is concerned with the application of recently developed epidemiological and statistical tools to inform the optimisation of a national surveillance strategy of considerable importance to human health. The results of a series of epidemiological investigations of surveillance strategies for zoonotic Salmonella are presented. Salmonella are one of the most common and serious zoonotic foodborne pathogenic bacteria globally. These studies were motivated by the increasing focus on the cost-effectiveness of surveillance while maintaining consumer confidence in food supply. Although data from the Danish Salmonella surveillance and control programme has been used in these investigations, the techniques may be readily applied to other surveillance data of similar quality. The first study describes the spatial epidemiological features of Danish Salmonella surveillance and control programme data from 1995 to 2004, using a novel method of spatially adaptive smoothing. The conditional probability of a farm being a case was consistently high in the the south-west of Sonderjylland on the Jutland peninsula, identifying this area for further investigation and targeted surveillance. The identification of clustering of case farms led into the next study, which closely examines one year of data, 2003, for patterns of spatial dependency. K-function analyses provided evidence for aggregation of Salmonella case farms over that of all farms at distances of up to six kilometres. Visual semivariogram analyses of random farm-level effects from a Bayesian logistic regression model (adjusted for herd size) of Salmonella seropositivity, revealed spatial dependency between pairs of farms up to a distance of four kilometres apart. The strength of the spatial dependency was positively associated with slaughter pig farm density. We describe how this might inform the surveillance programme by potentially targeting herds within a four kilometre radius of those with high levels of Salmonella infection. In the third study, farm location details, routinely recorded surveillance information, and industry survey data from 1995 were combined to build a logistic seroprevalence model. This identified wet-feeding and specific pathogen free herd health status as protective factors for Salmonella seropositivity, while purchasing feed was a risk factor. Once adjusting for these covariates, we identified pockets of unexplained risk for Salmonella seropositivity and found spatial dependency at distances of up to six km (95% CI: 2–35 km) between farms. A generalised linear spatial model was fitted to the Jutland data allowing formal estimation of the range of spatial correlation and a measure of the uncertainty about it. There was a large within-farm component to the variance, suggesting that gathering more farm level information would be advantageous if this approach was to be used to target surveillance strategy. The fourth study again considers data from the whole study period, 1995 to 2004. A detailed temporal analysis of the data revealed there was no consistent seasonal pattern and correspondingly no benefit in targeting sampling to particular times of the year. Spatiotemporal analyses suggested a local epidemic of increased seroprevalence occured in west Jutland in late 2000. Lorelogram analyses showed a defined period of statistically significant temporal dependency, suggesting that there is little value in sampling more frequently than every 10 weeks on the average farm. The final study uses findings from the preceding chapters to develop a zero-inflated binomial model which predicts which farms are most at risk of Salmonella, and then preferentially samples these high-risk farms. This type of modelling allows assessment of similarities and differences between factors that affect herd infection status (introduction) and those that affect the seroprevalence in infected herds (persistence and spread). The model suggested that many of the herds where Salmonella was not detected were infected but at a low prevalence. Using cost and sensitivity, we compared the results with those under the standard sampling scheme based on herd size, and the recently introduced risk-based approach. Model based results were less sensitive, but showed significant cost savings. Further model refinements, sampling schemes, and the methods to evaluate their performance are important areas for future work, and should continue to occur in direct consultation with Danish authorities.

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10

Mason, Susan Leigh. "Metabolism of triacylglycerol-rich lipoproteins in sheep." Lincoln University, 1991. http://hdl.handle.net/10182/1756.

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This thesis describes two approaches for studying of lipoprotein metabolism in sheep. The first approach involves the assay of lipoprotein lipase (LPL) activity to determine the role of lipoprotein-triacylglycerol fatty acids in fat deposition in sheep. This enzyme is the rate limiting enzyme in the hydrolysis of fatty acids from lipoprotein-triacylglycerol. The second approach was to characterize and quantify in vivo lipoprotein metabolism using iodinated very low density lipoprotein (¹²⁵I-VLDL) and low density lipoprotein (¹³¹I-LDL). Cross-bred lambs were divided into two treatment groups and either weaned early at 5 weeks of age or remained suckling. Lambs were slaughtered at 12 or 23 weeks at which time the body composition and adipose tissue LPL activity were determined. The differences in rearing led to differences in body composition. The suckled lambs were larger and fatter than weaned lambs. The increased fatness in the suckled lambs was associated with increased LPL activity (U/mg protein) in subcutaneous adipose tissue and was reflected in higher LPL activity in post-heparin plasma (PHP) taken 2 days prior to slaughter. The role of insulin in the regulation of LPL activity was investigated by either infusing a subset of the weaned and suckled lambs with insulin for 7 or 18 weeks or using the euglycemic clamp technique to study the effect of short insulin infusions. The long term infusion of insulin had no significant effect on PHP LPL or on adipose tissue LPL (U/g tissue). However, after infusing insulin for 6h at 6.3 mU.kg⁻·⁷⁵.h⁻¹ during the euglycemic clamps, a two fold increase in LPL activity in biopsied subcutaneous adipose tissue was observed. In the second approach, in vivo lipoprotein metabolism was investigated in 4 lambs using apolipoprotein B as a marker. Following the simultaneous injection of ¹²⁵I VLDL and ¹³¹I VLDL, the specific activities of apoB in VLDL, IDL and LDL fractions were determined. ApoB specific activity curves demonstrated that VLDL is metabolised to IDL and subsequently to LDL. The turnover of VLDL-B (3.45mg.d⁻¹.kg⁻¹) and LDL-B (4.8mg.d⁻¹.kg⁻¹) was calculated by fitting the VLDL-¹²⁵I-B and LDL-¹³¹I-B specific activity data to a mono-exponential equation. The metabolism of lipoproteins, inferred from the study of apoB, was shown to be similar in sheep to that reported in other animals although the amount of lipoprotein synthesised was low. A model to describe the kinetics of apoB metabolism in sheep was developed using SAAM. The proposed model features a three pool delipidation chain for VLDL, and subsystems containing two pools for IDL and LDL. IDL may be catabolised to LDL or cleared directly from the plasma. The developed model can now be used to compare the metabolism of lipoproteins in different physiological states and to design new experiments to study lipoprotein metabolism further.

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11

Greer, Andrew W. "Estimates of the nutritional cost of the development of immunity to gastrointestinal parasites in sheep." Lincoln University, 2005. http://hdl.handle.net/10182/11.

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This thesis describes a series of three experiments designed to estimate the nutritional cost of the immune response to the gastrointestinal nematodes Trichostrongylus colubriformis and Teladorsagia circumcincta in sheep. For each experiment, animals were allocated hierarchically by liveweight into one of four groups that were either infected (group IF), similarly infected and concurrently immuno-suppressed with weekly intramuscular injections of 1.3mg kg liveweight (LW)⁻¹ of methylprednisolone acetate (group ISIF), immunosuppressed only (group IS) or remained as controls (group C). Body composition of all animals was estimated using x-ray computer tomography prior to infection and at the conclusion of each study with bodyweight and faecal nematode egg counts (FEC; eggs gram⁻¹ of fresh faeces (epg)) measured along with blood samples taken for the determination of levels of serum proteins, phosphate and antibodies. In the first trial (Chapter 3), the nutritional cost of both the acquisition and maintenance of immunity to gastro-intestinal nematodes was investigated using immunologically naive 5-month-old lambs and immunologically competent 17-month-old ewes during infection with 2,000 and 4,000 L3 infective T. colubriformis larvae d⁻¹, respectively (80 L3 T. colubriformis larvae kgLW⁻¹ d⁻¹). Profiles of FEC and comparative worm burdens at slaughter indicated an effective immune response was maintained in IF ewes and developed in IF lambs while successfully suppressed in both ISIF lambs and ISIF ewes and was confirmed by serum antibody titres. The typical reduction in voluntary feed intake as a consequence of infection was observed in IF lambs (0.30, p<0.001) but not in IF ewes, ISIF lambs or ISIF ewes, and appeared to be associated with L3 IgA. Gross efficiency of use of metabolizable energy (ME) for net energy (NE) deposition was reduced by 0.20 in lambs during acquisition of immunity and by 0.16 in ewes maintaining an established immunity. Infection in immuno-suppressed animals reduced efficiency by 0.05 and 0.15 for lambs and ewes. These findings allowed the hypothesis that the reduction in feed intake and nutrient utilization in young parasitized sheep is caused by physiological signalling associated with the acquisition phase of the host immune response to infection, rather than simply the damage caused by the parasite per se. The second trial (Chapter 4) investigated the influence of metabolizable protein (MP) supply on the metabolic disturbances associated with the acquisition phase of the immune response during infection with 2,000 L3 T. colubriformis d⁻¹. Groups of lambs were offered either a low protein (L; 62g MP kgDM⁻¹) or high protein diet (H; 95g MP kgDM⁻¹). Patterns of total daily egg excretion indicated that an effective immune response was developed in HIF, but not LIF, HISIF nor LISF and was confirmed by comparative worm burdens. The proportionate reduction in feed intake in immunologically normal animals was reduced through the provision of additional protein, being 0.12 in HIF and 0.23 in LIF. Regardless of diet, infection did not cause a reduction in feed intake in immuno-suppressed animals (p>0.05). Infection proportionately reduced the gross efficiency of ME utilization in immunologically normal animals by 0.23 in HIF (p=0.09) and by 0.51 in LIF (p=0.01), but not in immuno-suppressed animals. Immuno-suppression did not suppress serum L3 IgA levels in seven of the eight HISIF and four of the eight LISIF animals. Furthermore, only four out of the eight immunologically normal animals from both the HIF and LIF groups displayed an L3 IgA response. Consequently, regardless of immunosuppression treatment, animals were termed as IgA responders (HR or LR) or non-responders (HN or LN). Feed intake was proportionately reduced from day 22 by 0.15 in HR (p=0.03) and by 0.32 in LR (p=0.01), but was not significantly reduced in HN or LN. Gross efficiency of ME utilization was significantly reduced for LN animals only, being proportionately 0.59 (p<0.01). These findings allowed the conclusion that additional MP reduced the consequence of immunological signalling that was displayed in reduced feed intake and in nutrient utilization, both of which appeared to be associated with an IgA response. It is hypothesized that the lessening of nutritional disturbance observed in high protein and immuno-suppressed animals could be a consequence of altered physiological signalling during the immunological cascade. The third trial (Chapter 5) utilized lambs infected with the abomasal parasite T. circumcincta to explore the possibility that the reduction in feed intake and nutrient utilization is a universal phenomenon of the acquisition phase of the immune response to nematode parasites inhabiting different organs along the gastrointestinal tract. In addition, immunological changes at the site of parasite infestation in the abomasal mucosa were measured from serial biopsy tissue samples taken from a further twelve animals that were surgically fitted with an abomasal cannula and either infected (CIF) or concurrently infected and immuno-suppressed as described previously (CISIF). The development of immunity in IF animals was accompanied by a 0.17 proportional decrease in feed intake between days 15 to 28 of infection (p<0.05) and a 0.20 proportional reduction in nutrient utilization (p=0.07), none of which were observed in ISIF animals. While FEC and worm burdens indicated successful immunosuppression in ISIF animals, both serum IgA and total antibody production were not reduced. The development of immunity in CIF was reflected in an increase in both mast cells and globule leukocytes in serial abomasal tissue biopsies, both of which were reduced in CISIF (p<0.01 for both). In serial biopsy tissue, immuno-suppression did prevent a rise in tissue IgA that was apparent in CIF animals (p<0.01) although these changes were not reflected in serum IgA levels. It appears that the alleviation of the reduction in feed intake and nutrient utilization in young lambs through the use of corticosteroid induced immuno-suppression may be a universal phenomenon for both intestinal and abomasal parasites, but the association with and/or role of IgA during infection with T. circumcincta is unclear. In summary, the reduction in feed intake and nutrient utilization in sheep during infection with both the abomasal nematode T. circumcincta and the small intestine nematode T. colubriformis appears to be associated with a component(s) of the acquisition phase of the host immune response, rather than, as conventionally assumed, the direct mechanical damage of the parasite per se. It is hypothesised that the nutritional disturbance as a consequence of infection in young lambs may be the result of pro-inflammatory cytokines involved in immunological signalling that may also be associated with the production of IgA, the effects of which can be reduced through the provision of adequate MP. These studies provide evidence that the immune response to gastrointestinal parasites is nutritionally costly to the animal and have implications for application of manipulations that are intended to promote the development of a strong immune reaction in high producing animals.

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12

Muraki, Tomohiro. "Effects of alternative grass species on grazing preference of sheep for white clover." Diss., Lincoln University, 2008. http://hdl.handle.net/10182/1095.

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Despite the importance of a high white clover (Trifolium repens) content in temperate pastoral systems in terms of livestock performance and nitrogen fixation, the proportion of white clover in grass-clover pastures is often low (<20%). This thesis examined in two experiments whether the white clover content of pastures could be improved by sowing white clover with alternative grass species to diploid perennial ryegrass (Lolium perenne L.). In a pasture experiment, DM production, pasture composition and morphology of grass-clover mixtures was measured over the establishment year (January 2007 to January 2008) where white clover was sown in fine mixtures with diploid perennial ryegrass, tetraploid perennial ryegrass, timothy (Phleum pratense L.) and cocksfoot (Dactylis glomerata L.). Pastures were irrigated and rotationally grazed with on-off grazing with Coopworth ewe hoggets. Total annual DM production of pasture was more than 20% higher in tetraploid (12521 kg DM ha⁻¹) and diploid (11733 kg DM ha⁻¹) perennial ryegrass than timothy (9751 kg DM ha⁻¹) and cocksfoot (9654 kg DM ha⁻¹). However, timothy (5936 kg DM ha⁻¹) and cocksfoot (5311 kg DM ha⁻¹) had more than four times higher white clover annual DM production than tetraploid (1310 kg DM ha⁻¹) and diploid (818 kg DM ha⁻¹) ryegrass. Pasture growth rate at the first three harvests in autumn was significantly greater in tetraploid and diploid ryegrass than timothy and cocksfoot. Timothy and cocksfoot had a higher proportion of white clover than tetraploid and diploid perennial ryegrass throughout the entire year. This was due to more and larger white clover plants in timothy and cocksfoot plots. In a grazing preference experiment, the partial preference of sheep for white clover offered in combination with the same grass species as in the pasture experiment was measured in five grazing tests in May, September, October, November and December 2007. Pastures were sown in January 2007. Paired plots (grass and clover both 4.2 m x 10 m) were grazed by three Coopworth ewe hoggets between 9am and 5pm, and preference was recorded by decline in pasture mass and visual scan sampling for grazing time. Grazing preference for clover was generally low throughout these tests (e.g. average apparent DM intake from clover = 47%; average grazing time from clover = 44%). Several explanations are proposed for this low preference including a high N content and intake rate of the grass relative to the clover. No significant differences were found among the grass treatments in total grass grazing time, total clover grazing time, ruminating time, the proportion of grazing time on clover, selective coefficient for clover and DM intake percentage from clover at any date. There was no significant change in overall sward surface height (SSH) decline among grass treatments throughout all the tests except December 2007 when the overall SSH decline for cocksfoot was significantly lower than the other species. The study indicated that the rapid growth rate of perennial ryegrass in the early phase of pasture establishment, rather than differences in partial preference, was the key factor limiting white clover content in the mixed swards relative to cocksfoot and timothy pastures. It is concluded that high clover-containing pastures capable of delivering high per head performance can be established through the use of slow establishing pasture species such as timothy and cocksfoot.

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13

Gudex, B. W. "Sireline variation in neonatal lamb cold tolerance." Lincoln University, 2001. http://hdl.handle.net/10182/1055.

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The cost of lamb mortality caused by cold exposure has been estimated at approximately 40 million dollars per year. This value is probably conservative as it does not include the cost due to the reduction in productivity in hypothermic lambs that manage to survive or the cost of reduced selection potential incurred by fewer lambs surviving until selection. The objectives of this research was to investigate whether sire-line variation exists in neonatal lamb cold tolerance and whether polymorphism in the β₃ adrenergic receptor gene can be used as a genetic marker for lamb cold tolerance and lean muscle growth. The influence of the climate, birthweight, age of dam at lambing, gender and birth rank on neonatal lamb cold tolerance was also analysed. Neonatal lamb mortality due to cold exposure was analysed in four field trials that used neonatal lamb morality from cold exposure as a predictor of neonatal lamb cold tolerance. Sire-line variation in neonatal lamb morality was observed in all trials, though it appeared that this effect was largely mediated through sire-line variation in lamb birth weight. Variation in lamb birth weight between birth rank classed was also found to be responsible for the influence of birth rank on neonatal lamb mortality due to cold exposure. The age of dam at lambing and the lamb gender was not observed to influence neonatal lamb mortality due to cold exposure. The sires from the cold tolerance study and the progeny of the lean muscle growth study were genotyped for the β₃ adrenergic receptor locus. Other studies have found evidence that a major gene exists in the catecholamine stimulation of brown adipose thermogenesis and evidence that the β₃-AR gene is a likely candidate. However, this hypothesis and the hypothesis that polymorphism in the β₃-AR gene is also linked to lean muscle growth in lambs was not confirmed in this study. So while it appears that the results were confounded by experimental design, there is evidence that influence of polymorphism in the ovine β₃ AR gene on neonatal lamb mortality and/or lean muscle growth is not sufficient to be considered a major gene effect. The implications of this experiment on the sheep industry and sheep farmers in general are huge. While completely eliminating lamb deaths due to inadequate cold tolerance is impossible, this study shows that large gains in lamb survival could be possible through selective breeding.

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14

Iposu, Shamsideen Oladeinde. "Effect of suckling on response to nematode parasites in young lambs." Diss., Lincoln University, 2007. http://hdl.handle.net/10182/115.

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The series of experiments described in this thesis were designed to investigate the role of suckling or late weaning in the response of young lambs to nematode infection. All experiments were conducted outdoors with grazing animals and no supplementation but for suckled groups of lambs whose counterparts were weaned to ryegrass – white clover swards. The parasite of interest was mainly Teladorsagia circumcincta solely but with mixed infection of Trichostrongylus colubriformis in one instance. In Chapter 3 (first experiment), the hypothesis that milk per se may have a direct effect on nematode development, rather than an indirect effect through enhancement of host immunity by superior nutrient supply was tested. Sixty, twinborn lambs were used, allocated to one of eight groups formed by either dosing lambs from 42 days of age or not with the equivalent of 1000 or 250 L₃ T. circumcincta larvae d⁻¹ until five days before necropsy, while a twin was either weaned at 39 days of age, suckled as single or twin until necropsy on day 84. The possibility that weaning one of a twin set onto pasture in close proximity to the ewe would cause abnormal ewe and lamb behaviour was tested by replicating the work in twins maintained as twins but in which one twin received equivalent of 250 and the other 1000 L₃ T. circumcincta larvae d⁻¹. This showed no abnormal ewe nursing or lamb suckling behaviour as a result of weaning a twin in a set. Relatively low faecal egg counts (FEC) and a two to three fold lower worm burdens suggest suckling could reduce larval establishment. Inability to detect peripheral titres of immunoglobulins supports this conclusion. An intra worm-population regulation of T. circumcincta, indicated by a pattern of greater egg-laying by a numerically smaller but physiologically better developed nematode population in suckled lambs measured in eggs 'in utero' and worm length made interpretation of FEC difficult. Suckling significantly improved weight gain and carcass weights, but early weaning did not reduce resilience to infection. In Chapter 4 (second experiment), 40 pairs of twin lambs, average age of 39 days, were either infected with the equivalent of 1000 L₃ T. circumcincta larvae d⁻¹ or not, while one twin was weaned and the other allowed to continue suckling. Necropsy was carried out on groups of five and six lambs from each of the uninfected and infected treatments, respectively, at mean age of 84, 112, and on six lambs from each group at 140 days of age. This serial slaughter allowed further confirmation of the hypothesis in Chapter 3 but also investigated the long-term effect of suckling on resistance or resilience of lambs at the trial when immune responses were anticipated to be developing. An in vitro direct larval challenge (IVDC) study, to monitor larval establishment, was carried out on tissue explants from necropsied lambs. Suckled lambs consistently showed lower FEC (P < 0.05) and worm burdens (P < 0.05) at every phase of the trial. Within the infected groups, % in vitro larval rejection suggested earlier immune responses in the weaned lambs by day 84, which was not consistent with lower worm burdens in suckled lambs but appeared similar in the subsequent necropsies. Lambs continued to show better growth due to suckling while weaning did not reduce the resilience of lambs confirming observations in Chapter 3. The immunoglobulin profile suggested the commencement of immune responses in lambs from the period after the 84th day necropsy, with significantly greater (P < 0.01) IgA titre in the infected groups, and the suckled lambs towards the end of the trial on day 140. A vaccinating effect of early exposure to parasites was coincidentally revealed as a result of unintentional pasture larval contamination, seen in suckled non-infected lambs shedding fewer eggs and harbouring fewer worms during the later necropsies compared with their weaned non-infected counterparts. In Chapter 5 (third trial), 93 pairs of twin lambs, 47 pairs of which received a vaccinating mixed infection of T. circumcincta and T. colubriformis larvae (60 L₃ / kg W / d) at ratio 40:60, respectively during the period 36 – 103 days of age, were either weaned early on day 51 or later on day 108. All lambs were drenched on day 108 and groups received challenge infections from day 116, at same rate with the vaccinating infection, or not, which ceased five days before respective necropsies. Necropsies were carried out on selected lambs on days 108, 184 and 218. The direct effect of milk on larval establishment appeared to feature only in the T. circumcincta populations on slaughter day 108. The long-term benefit of late weaning for development of resistance was conditional on lambs receiving the vaccinating infection, and appeared to be more pronounced in the small intestine, reflected by a greater reduction of T. colubriformis populations in that organ than of T. circumcincta populations in the abomasum. A negative consequence of enhanced immune response was the suggestion of an increased metabolic cost in reduced performance of lambs. In conclusion, the work provides support to the hypotheses that: (a.) suckling may reduce the establishment of nematode larvae through the direct effect of milk, (b.) may enhance rapid development of host immunity to infection, and (c.) it further suggests that lack of larval experience during suckling may have long term negative implications for host resistance. Finally, it suggests that milk may play little role in the enhancement of host resilience to infection and, on the contrary, that additional metabolic cost may be associated with a more rapid development of immunity resulting from larval challenge while suckling.

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15

Al-Amer, Saleh Suliaman. "Nutritional and toxicological studies on New Zealand mutton bird meat (Puffinus griseus)." Lincoln University, 2009. http://hdl.handle.net/10182/1659.

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New Zealand mutton bird or tītī (Puffinus griseus and order procellariiformes) nest in New Zealand during the summer months, migrate to the northern hemisphere during May and return in September. Their eggs are laid during November and December and the chicks are hatched in the following January and February. Large numbers of them are harvested from April to May in New Zealand. They are wild seabirds annually harvested by Maori according to the customary rights agreement set by Treaty of Waitangi.NZ mutton birds also called Sooty Shearwaters are noted for their high proportion of body fat.These birds are interesting since its sole diet is based on krill and other small marine organisms that are potentially rich in n-3 fatty acids and other marine bioactive compounds. The proximate composition, fatty and amino acids and cholesterol content of mutton bird pectoral muscle were determined and compared with other common meat to explore the nutritional value of this New Zealand delicacy. The concentration of twenty two essential and toxic elements including silver (Ag), aluminium (Al), arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), mercury (Hg), manganese (Mn), lead (Pb), selenium (Se), potassium (K), calcium (Ca), magnesium (Mg), boron (B), iron (Fe), nickel (Ni), sulphur (S), sodium (Na) and cobalt (Co) and zinc (Zn) in mutton bird breast meat (Puffinus griseus) were measured over two years to evaluate its safety for human consumption. Persistent organochlorine, dichlorodiphenyltrichloroethane (DDT) and their metabolites, and aldrin and lindane were also measured.Twenty bird carcasses were purchased in both 2006 and 2007 from a local source. Meat samples from the pectoral muscle of two carcasses were pooled to generate 10 samples for each year. These were used for trace element analysis using inductively coupled plasma-mass spectrometry (ICP-MS). Trace elements were in the range of 0 to 1.09 mg/kg wet weight for Ag, 0 to 3.32 for Al, 0.17 to 0.79 for As, 0.01 to 0.07 for Cd, 0.03 to 0.15 for Cr, 3.56 to 4.88 for Cu, 0 to 0.15 for Hg, 0.22 to 0.50 for Mn, 0 to 0.09 for Pb, 0.66 to 1.18 for Se and 11.49 to 23.70 for Zn. In 2006, Ag, Al, Mn and Zn concentrations were significantly higher but Pb and Hg concentrations significantly lower compared to the 2007 samples (P < 0.05). Apart from one sample in 2006, all the samples were below the published maximum level for concern. However, our preliminary data indicated that the higher level of Cd and other metals in the skin of mutton bird may compromise the overall safety to humans consuming the skin of mutton birds. It is suggested that the evaluation of the metals in different parts and/or the whole mutton bird at different seasons is required to assure complete safety to the consumers.Furthermore, the nutritional value of mutton bird meat was studied over two harvesting seasons (2006 and 2007) to investigate the impact of seasonal variation. The moisture and carbohydrates contents ranged between 54.0 to 55.0 % and 2.8 to 3.0 %, respectively, and no seasonal effects were evident in these components. The values for fat and ash contents were higher and the protein content lower for birds harvested in 2007 compared with the 2006 values which ranged from 11.8 to 13.0, 10.3 to 11.7, and 20.3 to 18.5 % for fat, ash and protein content respectively. The major amino acids in mutton bird pectoral muscle were glutamate, aspartate, lysine, leucine, and arginine. Higher lysine concentrations and lower proline, cystein and methionine were found in mutton birds compared with the literature values for beef, lamb and pork. The essential amino acid content in mutton bird (43.8 and 44.9 % in 2006 and 2007, respectively) was slightly higher than those found in beef and lamb meats (42-43%).The major fatty acids detected were palmitic (C16:0), stearic (C18:0), oleic and isomers (C18:1), eicosenoic (C20:1), Docosahexaenoic acid (DHA) (C22:6), icosapentaenoic acid (EPA) (C 20:5) and these accounted for approximately 77% of the fatty acids. The 3/6 ratio of fats from pectoral muscle was 1.3. The cholesterol concentration varied slightly in the two years with 184.4±37.37and 134.4±25.55mg/100 g fresh weight for 2007 and 2008 respectively. Mutton bird was shown to contain significantly higher cholesterol content (134.4-184.4) than other common meat such as chicken (80.3-88.9), lamb (62.3), fish (52.79) and beef (51.97). Overall, the nutritional value of mutton bird muscle was similar to or superior to the traditionally protein sources such as seafood and red meat. Annual variations existed in the composition of Mutton bird pectoral muscle but this is not of nutritional consequence but might be a useful indicator for ecological events such as feed availability and other environmental issues. Mutton bird seems to be a good source of essential minerals, Zn and Fe compared with other traditional meats source. Mutton bird meat is nutritionally as good as the major sources of red or white meats. It may even have advantages over the other common meats (beef, lamb, fish and chicken) due to its high protein and monounsaturated fatty acids (omega n-3 and n-6) content. However, its high cholesterol content may represent a risk factor for some people.

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16

Ludemann, M. R. "Development of an experimental approach to measure vitamin B12 production and absorption in sheep." Lincoln University, 2009. http://hdl.handle.net/10182/1424.

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Clinical diagnosis of vitamin B12/cobalt (Co) deficiency is difficult due to the unspecific nature of the clinical symptoms. The apparent increase in vitamin B12 deficiency in New Zealand in the late 1990’s made it clear that health providers were very reliant on plasma reference ranges to diagnose deficiency. However, the lack of quantitative data of what these reference ranges represent in terms of supply of vitamin B12, has prevented a better understanding of the metabolism of vitamin B12 within sheep. This thesis describes the development of an experimental approach to measure vitamin B12 production and absorption in sheep. The model was then used to investigate whether the type of carbohydrate source affects vitamin B12 production and/or absorption. In the first trial (Chapter 4), an adaptation of the repletion technique of Suttle (1974) for copper was used. Previously vitamin B12 depleted sheep were maintained on a diet of 400 g DM meadow hay and 250 g DM crushed barley and which provided a daily intake of 0.03 mg Co. Sheep were intravenously infused with known quantities of vitamin B12 (0 – 200 nmol hydroxycobalamin/d) to determine whether a relationship exists between plasma vitamin B12 concentration and rate of entry of the vitamin into the bloodstream and to quantify the relationship. Eight ewes fitted with rumen and abomasal cannulae were used in a paired 4x4 Latin square design. Four levels of vitamin B12 were infused into the jugular vein for 4 d followed by recovery period of 17 d and the plasma responses measured during this period. Control animals maintained relatively stable plasma levels while levels in treated animals rose sharply within 4 h after the start of infusion. The responses were proportional to infusion rate with a highly significant difference in concentrations observed between treatments (P<0.001). Appearance of vitamin B12 in the plasma was quantified by the relationship Y(pmol/l) = 238 + 102x0.74 where x = infusion rate(nmol B12/d), R² = 0.945. Plasma levels declined rapidly on cessation of infusion and had returned to original pre-treatment levels by the end of the recovery period. The second trial (Chapter 5) quantified plasma responses in relation to dietary Co supply to the rumen. The same eight rumen and abomasal cannulated ewes were used in a paired 4x4 Latin square with 4 levels of intra-ruminal Co infusion in amounts ranging from 0 – 1 mg Co/d as CoSO₄, infused for 4 d followed by a 19 d recovery period. Plasma vitamin B12 response was measured as were concentration of vitamin B12 in abomasal digesta supernatant and digesta liquid flow, the latter using polyethylene glycol as liquid phase marker. Plasma concentration increased from approximately 300 pmol/l to 750 – 800 pmol/l, peaking at day 5 after commencement of infusions. Control animals infused with water maintained base-line plasma vitamin B12 levels around 250 pmol/l. Digesta liquid flow was estimated at 14.6 l/d. The results from Chapters 4 and 5 allowed estimates of vitamin B12 production and absorption. These results are discussed and efficiency of capture of Co into vitamin B12 from dietary Co intake was estimated to decrease from 8.4% to 0.74% as Co intake increased from 0.03 to 1.11 mg Co/d. Absorption coefficient was estimated from the relationships between abomasal vitamin B12 flow and plasma response. The absorption coefficient was calculated to increase from 7 to 10% as abomasal vitamin B12 flow increased from 108 to 142 nmol/d. The current animal model was subsequently used in the final trial (Chapter 6) to investigate whether the type of carbohydrate supplied in the diet influences vitamin B12 production and absorption. The same eight ewes fitted with rumen and abomasal cannulae were used in a 2x4 cross-over design. Half of the ewes were maintained on the basal diet and crushed barley while, for the remainder, the barley was replaced by 250 g of fructose:sucrose in a 80:20 mix during a 7 d period of adaptation. While on their respective diets, both groups were then infused with 0.3 mg Co/d for 6 d, a level chosen to optimise plasma vitamin B12 response. All ewes were then returned to the basal diet and barley for a 15 d recovery period after which treatments were reversed and the procedure repeated. Rumen pH, valeric, propionic, butyric, succinic, and acetic acid and ammonia concentrations were not significantly affected by diet. Digesta flow was 30% greater on a diet enriched with starch compared to sugar-enriched diet. There was an observed difference in vitamin B12 production. However there was no significant diet x time effect on plasma vitamin B12 concentration so any difference in production was negated by a difference in absorption. Carbohydrate supplied as water soluble carbohydrate or starch does not appear to influence plasma vitamin B12 concentration.

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Penny, G. M. "Supply chain (re)alignment in New Zealand’s sheep meat and dairy industries : knowledge, networks and learning at the farmer-processor site." 2003. http://hdl.handle.net/2292/2418.

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Emerging from the agricultural crisis of the 1988s, producers and processing companies in New Zealand's sheep meat and dairy industries, found themselves in an evolving neo-liberalised environment. By the late 1990s major structural (re) alignment had taken place in production and processing to accommodate shifts in markets and regulation, centring on 'food safety', 'quality' and "availability" For both farmers and processing company staff, this meant venturing into unfamiliar jointly occupied spaces and unchartered relational territory, Through a participatory research approach using multiple methodologies, this thesis examines supply chain (re)alignment at a macro and micro-scale, focussing on the farmer-processor relationship and knowledge, network and learning processes of farmers in (global) lamb and dairy supply chains in New Zealand. In seeking to account for evolving agri-food chain relations at multiple scales, this research turns to the global commodity chains (GCC) literature and draws on pragmatic solution-oriented ideas emerging from the developing field of Supply Chain Management (SCM). It also includes theoretical input from the cognitive and behavioural sciences to interpret the empirical data on farmer's knowledge, networks and learning in different supply chains, which it argues are key features of globalising agri-food economies. In this research these literatures and theories are enveloped by a broader yet, incomplete, theoretical foundation - that of evolutionary political economy (EPE), which is extended in this thesis, The thesis argues that an EPE framework provides a useful window on the governance of New Zealand's relations at a distance because it allows the specificity of micro-scale coordination activities and relations (in jointly occupied spaces) in New Zealand to be embedded in local and macro scale governance regimes and historical development processes. The findings show global market and regulatory pressures continue to drive supply chain (re)alignment in New Zealand, and chain building is occurring in different ways at the farmer-processor interface, between and within the different industries. Farmers' place specific on-farm knowledge co-evolves with off-farm knowledge through a combination of concrete experience, trial and error, socialisation and reflection, with both on and off farm knowledge becoming more overt in practice and strategy as supply chain specifications becomes more precise. Farmers utilise a range of networks (informal, formal, specialist and general), which serve as both information channels and learning forums. New Zealand's agricultural industries are like many in competitive globalising economies -'new' know ledge is being generated and used to broker global and local social, economic and environmental contexts and values, and in the process, supply chain partners' capacities and relations evolve.

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Amerah, Ahmed M. "Feed particle size, whole wheat inclusion and xylanase supplementation in broiler diets : influence on the performance, digesta characteristics and digestive tract development : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Poultry Nutrition at Massey University, Palmerston North, New Zealand." 2008. http://hdl.handle.net/10179/1586.

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The first three experiments of this thesis examined the effects of particle size reduction of grains in relation to feed form (mash vs. pellet), grain type (wheat vs. maize) and xylanase supplementation on broiler performance, energy utilisation, digestive tract development and digesta parameters. The fourth experiment examined the interaction between wheat hardness and xylanase supplementation. The effects of insoluble fibre source and whole wheat inclusion were studied in the fifth experiment. In the first experiment (Chapter 4), pelleting reduced nitrogen-corrected apparent metabolisable energy (AMEn), but broiler performance was superior in birds fed pelleted wheat-based diets compared to those fed mash diets. Feed form had a greater effect on various measured parameters than did particle size. Pelleting evened out differences in particle size distribution between treatments and, as a result, wheat particle size had no effect on the performance of broilers fed pelleted diets. In contrast, the second experiment (Chapter 5) showed that differences in particle size distribution persisted between diets after pelleting and, as a result, coarse grinding of wheat or maize improved broiler performance compared to those fed diets based on fine particles. These results may be related, in part, to changes in size distribution following pelleting. In mash diets, inconsistency in performance reponses were found. In the first experiment (Chapter 4), coarse grinding of wheat improved weight gain and feed per gain compared to medium grinding. In the third experiment (Chapter 6), however, grinding particle size had no influence on broiler performance. The observed discrepancy suggests involvement of other factors such as wheat cultivar and grain hardness. Data reported in Chapter 6 showed that xylanase supplementation improved feed per gain of birds fed the coarse particle size diet, but had no effect on those fed the medium particle size diet. In Chapter 7, there was a significant interaction between wheat hardness and xylanase supplementation due to the improved feed per gain and AMEn of birds maintained on hard wheat-based diet, while there was no effect of xylanase on sort wheat-based diet. These findings suggest that the efficiency of exogenous enzymes is influenced by both particle size and wheat hardness. Data reported in Chapter 7 showed that inclusion of soft or hard whole wheat pre-pelleting produced different particle size distributions in the pelleted diets. This suggested that hardness of the grain must be considered when choosing whole wheat for inclusion in broiler diets. Data on the effect of feed particle size on its subsequent distribution in poultry digesta are scanty. Results reported in Chapters 4 and 5 showed that there was no effect of feed particle size within feed form on duodenal digesta particle size. On the other hand, particle size of duodenal digesta was influenced by feed form (mash vs. pellet). Wheat hardness was also found to influence the particle size of proximal (duodenum and jejunum) intestinal digesta (Chapter 7). These results indicated that the gizzard does not uniformly reduce the size of all particles. However, the gizzard appears highly efficient in grinding large particles, although some large particles escape the grinding. The final experiment demonstrated that the effects of insoluble fibre on digestive tract development and broiler performance differed depending on the fibre source. Wood shavings, a source of coarse insoluble fibre, increased relative gizzard size and improved corrected feed per gain and ileal starch digestibility. In contrast, cellulose, a source of fine insoluble fibre, had no influence on these parameters. In conclusion, dietary manipulations, which stimulated gizzard development, positively influenced broiler performance and starch digestibility. The findings of this thesis suggest that energy savings during feed processing could be achieved by coarse grinding of grains with no adverse effect on broiler performance and that cereals used in broiler diets can be ground more coarsely than the current practice. Wheat hardness appears to be an important criterion to consider when choosing a cultivar for whole wheat inclusion in broiler diets. Another major finding was that the effectiveness of exogenous xylanase in wheat-based diets could be improved by considering factors such as particle size and wheat hardness.

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Okeoma, Chioma Maureen. "Studies on Neospora caninum and neosporosis in New Zealand cattle : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science, Massey University, Palmerston North, New Zealand." 2005. http://hdl.handle.net/10179/1643.

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Content removed due to copyright restrictions: Okeoma, C. M., Stowell, K. M., Williamson, N. B., & Pomroy, W. E. (2005). Neospora caninum: Quantification of DNA in the blood of naturally infected aborted and pregnant cows using real-time PCR. Experimental Parasitology, 110(1), 48-55. Okeoma, C. M., Williamson, N. B., Pomroy, W. E., Stowell, K. M., & Gillespie, L. M. (2004). Isolation and molecular characterisation of neospora caninum in cattle in new zealand. New Zealand Veterinary Journal, 52(6), 364-370. Okeoma, C. M., Williamson, N. B., Pomroy, W. E., Stowell, K. M., & Gillespie, L. (2004). The use of PCR to detect neospora caninum DNA in the blood of naturally infected cows. Veterinary Parasitology, 122(4), 307-315. Okeoma, C. M., Williamson, N. B., Pomroy, W. E., & Stowell, K. M. (2004). Recognition patterns of neospora caninum tachyzoite antigens by bovine IgG at different IFAT titres. Parasite Immunology, 26(4), 177-185.
The objective of this research was to investigate neosporosis in New Zealand cattle using experimental and molecular tools. The research also aimed to isolate and characterise an indigenous New Zealand isolate of Neospora caninum. A series of discrete projects were conducted to achieve the set objectives. A pilot study was first conducted and a wild-type strain of non-cytopathic bovine viral diarrhoea virus (BVDV) type 1 virus was isolated in vitro from a persistently infected heifer. The isolate was used to challenge heifers and the effect of experimental BVDV infection on heifers naturally infected with N. caninum was investigated. Results showed that heifers that were both BVDV and N. caninum seropositive produced 44.4% (8/ 18) damcalf pairs that were both BVDV and N caninum seropositive. Serologically, 66.7% (12/18) dam-calf pairs were seropositive to N. caninum antibodies, while 80% (8/10) dam-calf pairs were BVDV seropositive. A Polymerase Chain Reaction (PCR) study was also conducted and N. caninum DNA was detected in the blood of naturally infected aborting and pregnant heifers. Real-time quantification of N. caninum DNA in the blood of infected heifers showed a decrease of N. caninum DNA after abortion in the aborting group and an increase through gestation in the pregnant group. A study of antigenicity recognised 7 immunodominant (~18, ~25, ~33, ~35~36, ~45~46, ~47 and 60-62 kDa) and 5 minor antigens of N. caninum by cow sera. Three isolates of N. caninum (NcNZ 1 ,NcNZ 2 and NcNZ 3) were isolated from the brains of an infected cow, her calf and a stillborn calf. These isolates were confirmed as N. caninum by PCR, immunofluorescence antibody test and immunohistochemistry and were pathogenic to BALB/c mice.

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Nuijten, Wilhelmina Gerardina Maria. "Effects of dietary fish oil or other lipids and Sanovite on pig performance and pork quality : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Animal Science at Massey University, Palmerston North, New Zealand." 2010. http://hdl.handle.net/10179/1702.

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In this experiment, the effects of (1) lipid type (soy bean oil, tallow and fish oil), (2) the period the fish oil was provided and (3) a dietary supplement containing conjugated linoleic acid (CLA), selenium (Se), vitamin E and vitamin C on pig performance and pork quality were studied. Forty-eight female pigs (PIC hybrids, with a mean live weight of 16.19 kg ± 1.56 SD) were obtained from a single commercial operation in the North Island of New Zealand. The pigs were rank ordered by weight and assigned to one of six dietary treatment groups. The diet base was either a combination of animal and plant feedstuffs (AT and PTS), plant feedstuffs only (PO, POS) or plant feedstuffs combined with fish oil (PFSe and PFSl). The diets also differed depending on the presence or absence of the nutritional supplement SanoviteTM and a vitamin C supplement. SanoviteTM is a trademarked dietary supplement containing CLA (BASF, Auckland, New Zealand), organic Selenium (Alltech Inc., Nicholasville, KY) and vitamin E (Morel et al., 2008). Diets POS, PTS, PFSe, PFSl contained SanoviteTM and a vitamin C supplement. Diets PO and POS were used to establish the effect of the supplementation with SanoviteTM and the vitamin C supplement. Pigs fed diet PFSe received plant feedstuffs and fish oil with supplement between days 1 and 35 and then diet POS up to day 84. Pigs fed diet PFSl received diet POS between days 1 and 35; plant feedstuffs and fish oil with supplement between days 36 and 56 and then diet POS up to day 84. Pigs in group PFSe and PFSl both received the same total amount of fish oil per pig (2.52 l/ 2.31 kg). Between days 1 and 56 of the experiment grower diets were fed, and finisher diets were fed between days 57 and 84 of the experiment. The pigs were kept in pens of six, but fed individually twice daily (at approximately 8 am and 3.30 pm) according to a fixed feeding schedule. Water was available at all times. Individual feed intakes were measured daily and live weight recorded weekly. Faeces were collected once a day during two days in week five of the trail for digestibility determination. Carcass quality characteristics determined at the abattoir included carcass weight and back fat thickness as measured at the end of the slaughter line. Meat quality assessments were performed on the Semimembranosus muscle (SM) from one of the topside cuts of each pig. Measurements of fatty acid profile (loin and backfat) and the Se content (lean meat) were conducted in Singapore by Mrs. J. Leong (MSc). In this study plant or animal feedstuffs, lipid type, SanoviteTM and vitamin C supplementation had no significant effects on growth performance and carcass quality. There were no differences in apparent faecal digestibility characteristics for dry matter (DDM) and organic matter (DOM) in the un-supplemented animal (AT) and plant based (PO) diets. Lipid type had a significant effect on the digestibility of ash (DA), and an increased ratio of unsaturated fatty acid to saturated fatty acid resulted in increases in DDM and DOM. DDM and DOM increased when soybean and linseed oil (POS) were used instead of tallow (PTS) or fish oil (PFS). The main differences in DDM, DOM and DA were observed between diets PO and POS. A positive effect of selenium, vitamin E and CLA supplementation is suggested. Increased cooking temperatures reduced tenderness (higher mean, peak force, yield force and peak force – yield force) and increased cooking loss. There was a significant negative relationship between ultimate pH and relative lightness (L*). There were highly significant positive correlations between all three measurements of expressed juice, and there was a significant positive correlation between cooking loss at 60 and 70oC (P<0.01) but correlations between expressed juice values and cooking loss were not significant. The P-values for the contrasts for cooking loss at 70 0C were significant for all contrasts except for AT vs PO and PFSe vs PFSl. For all other contrasts, the P-values for cooking loss at 70 0C were significantly higher in group POS than for groups PO, PTS and PFSe+PFSl. The P-value for (Peak force – Yield force) at 70°C was significantly higher in samples from group PFSe than for samples from group PFSl. The P-value for the myofibrillar fragmentation index (MFI) was significantly higher for group AT in contrast to group PO. Group POS had a significantly higher P-value for MFI in contrast to groups PO, PTS and PFSe and PFSl. Group POS had a lower P-value for sarcomere length in contrast to groups PFSe and PFSl. Group PFSe had a significantly higher P-value for sarcomere length in contrast to group PFSl. Group PFSe had a significantly higher P-value for expressed juice percentage loss in weight in contrast to group PFSl. Supplementing with SanoviteTM increased the Se content (P = 0.002) in lean meat as analysed by J. Leong (2010, personal communication). In general it was concluded that an increase in the ratio of unsaturated fatty acid to saturated fatty acid (U/S) in the diet resulted in higher levels of unsaturated fatty acids in loin and backfat. The fatty acid profile in the diet reflected the fatty acid profile of pork. Backfat of pigs fed diets including soybean and linseed oil contained higher levels of linoleic and α-linolenic acids. Diets PO and POS were used to establish the effect of the supplementation of CLA. The backfat of pigs fed diet POS contained higher levels of CLA (C18:2-trans-10, cis-12) and α-linolenic acid than pigs fed diet PO. The loin of pigs fed diet POS contained higher levels of palmitoleic and linoleic acid and CLA (C18:2-cis-9, trans-11) and lower levels of oleic acid than pigs fed diet PO. The use of fish-oil as a lipid type resulted in the highest levels of eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) in loin and backfat. The loin and backfat of pigs fed fish-oil in the second part of the grower phase (PFSl) contained higher levels of EPA, DPA and DHA than pigs fed fish-oil in the first part of the grower phase (PFSe). By enriching the swine diet with long-chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA) it was possible to increase the EPA, DPA and DHA content of pork. Enriching pork with LC n-3 PUFA will contribute to achieving standards for adequate intake (AI), but might not be suitable to reach suggested dietary targets (SDT). In conclusion, it was possible to change the pork composition by dietary manipulation without compromising pig performance and meat quality. There were a few significant effects from treatments on meat quality characteristics, but differences reported in this study were small and relatively unimportant. A negative influence of the dietary regime on palatability and meat processing was expected, but these issues are beyond the scope of this experiment.

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21

Kshatri, Bhoj Bahadur. "Evaluation of multipurpose fodder trees in Nepal : a thesis submitted in partial fulfilment of the requirement for the degree of Doctor of Philosophy (Ph. D.) in Forestry, Institute of Natural Resources, Massey University, Palmerston North, New Zealand." 2007. http://hdl.handle.net/10179/1438.

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This PhD thesis consists of nine chapters describing aspects of the subsistence farms of western Nepal in general, and a need-based evaluation of multipurpose fodder trees (MFT) as a source of dry-season forage for ruminants in particular, as a basis for mitigating the current high rate of land degradation and loss of productivity in livestock production systems in the region. Understanding the complex farming systems that provide a living for 65% of the 27.1 million people in Nepal is the key to designing effective programmes of research and development. Evaluation methods include review of past work, farmers group workshops to identify current practice in the use of MFT in Nepal, studies on biomass production of Artocarpus lakoocha and Ficus glaberrima trees older than 50 years in Nepal and the propagation of F. benjamina, comparison of the feeding preferences of sheep for alternative browse species, and study of the nutritive value of alternative forage diets for lactating buffalo. Reviews showed 2.2 million cattle and 1 million buffalo are an extra burden to steep land where productivity is declining at the rate of 1.25% per year. Indigenous knowledge identified Ficus glaberrima with its three varieties (Maghe, Chaite and Jethe), A. lakoocha, F. benjamina and Bassia butyracea as the best four MFT for renovating degraded lands. A survey study showed significantly higher dry matter (DM) production by F. glaberrima than A. lakoocha (154 vs 91 kg DM /tree/year) during dry periods at low altitude (800 - 1000m). There was no significant difference in production of fat - corrected milk (FCM ) between buffalos eating A. lakoocha, F.glaberrima or a diet of 53% straw and 47% F. glaberrima (DM basis). Metabolisable energy balance (MJME/day) was greater in Artocarpus than Ficus, with the mixed diet intermediate (+1.60, -0.34 and -12.94 MJ ME/buffalo/day respectively, relative to requirements, P=0.0318). When fed together in an indoor trial, poplar (48% = 106 g DM/sheep/day) and willow (43% = 95 g DM/sheep/day) were preferred to Ficus benjamina (8% = 18 g DM/sheep/day) by sheep, reflecting the greater maturity and structural strength of leaves of Ficus. These results are used to develop recommendations for choice of MFT species and management strategies to improve the sustainability and productivity of livestock systems incorporating fodder trees

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Sherriff, Ryan Leith. "Use of decision science to aid selection of genetically superior animals : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palmerston North, New Zealand." 2010. http://hdl.handle.net/10179/1277.

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This thesis is concerned with a theoretical simulation model for pig breeding, as part of the ongoing search for the “perfect” genotype. The starting point is an additive model to investigate how accurately the classical, infinitesimal model predicts genetic gain for traits controlled by few loci and few alleles. This initial investigation demonstrates that the infinitesimal model is robust, providing that at least 15 loci are controlling a trait and there is symmetry in the allele distributions. A Genotype-Pig (GE-Pig) model is then developed to apply the additive effects of alleles on sub-phenotypic traits like maximum protein deposition, minimum lipid to protein content in the whole body, ad libitum digestible energy intake, energy for maintenance requirement and water content in the whole body. These parameters are then used in a nutrient partitioning simulation model to growth a pig and calculate traditional breeding traits such as average daily gain, feed conversion ratio, and backfat thickness for any combination of alleles. Three algorithms, Genetic Algorithm, Tabu Search, and Simulated Annealing, are used to investigate the GE-Pig model and find optimal combination of alleles for different dietary and selection objective situations. The two diets investigated were either of a low or high quality, and the three selection objectives used were, maximising average daily gain, minimizing feed conversion ratio, and minimizing back fat. A graphical method is developed for easy comparison of the genotypes. Of the algorithms, the Genetic Algorithm performed the best, followed by Tabu Search and finally Simulated Annealing. It is demonstrated that, in general, there is a different, single, optimum for any given selection objective and diet. However under the back fat selection objective, both diets produce the same optimal genotype. Also there are many similarities between the optima for the average daily gain and feed conversion ratio selection objectives. When the theoretical minimum number of generations of selection to the optima is considered, the feed conversion ratio selection objective is the quickest for a breeding program to achieve the optimal solutions, followed by back fat, then average daily gain. It is demonstrated that diet also has an effect on the theoretical number of generations. A Multiple selection objective, using relative economic values applied to the individual selection objectives, is also investigated. For both diets, the majority of the multiple selection objective solutions are in the vicinity of the feed conversion ratio optima, indicating that feed conversion ratio is the most prominent factor. It is also demonstrated that the optimal solution is most affected by the objective parameter weights under low diet conditions.

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23

Beausoleil, Ngaio Jessica. "Behavioural and physiological responses of domestic sheep (Ovis aries) to the presence of humans and dogs :|ba thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palmerston North, New Zealand." 2006. http://hdl.handle.net/10179/1484.

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Appendices removed due to copyright restrictions Appendix 1: Beausoleil, N J, Stafford, KJ, Mellor, DJ. 2005. Sheep show more aversion to a dog than to a human in an arena test. Applied Animal Behaviour Science, 91 : 2 1 9-232. Appendix 2: Beausoleil, NJ, Stafford, KJ, MelIor, DJ. Does direct human eye contact function as a warning cue for domestic sheep, Ovis aries? Journal of Comparative Psychology, J 20 (3) : 269-279. Appendix 3:Beausoleil, NJ, Stafford, KJ, MelIor, DJ. 2004. Can we use change in core body temperature to evaluate stress in sheep? Proceedings of the New Zealand Society of Animal Production, 64: 72-76. Appendix 4: Beausoleil, N.J, Mellor, DJ, Stafford, KJ 2004. Methods for marking New Zealand wildlife: amphibians, reptiles and marine mammals. Department of Conservation, Wellington, New Zealand 147p. ISBN 0-478-2263 1-4. Appendix 5: Mellor, DJ, Beausoleil, NJ, Stafford, KJ 2004. Marking amphibians, reptiles and marine mammals: Animal welfare, practicalities and public perceptions in New Zealand. Miscellaneous Publication, Department of Conservation, Wellington, New Zealand 55p. ISBN 0-478-22563-6.
Both humans and dogs are integral in sheep production systems; however, which is more aversive to sheep, or indeed, whether either causes significant stress, has not been shown experimentally. The aim of this thesis was to examine some behavioural and physiological responses of domestic sheep to the presence of humans or dogs. An arena test was used to measure the relative aversion of sheep to the presence of a human or dog, as well as to elucidate differences in the responses of flocks at the University of Western Australia (UWA) which were putatively selected for differences in fearfulness. A Y maze preference test was used to 'ask' sheep whether they preferred a human shaking a rattle or a barking dog. In both tests, adrenocortical responses were measured concurrently to support the interpretation of behaviour. The presence of a human or dog in the arena elicited significantly more avoidance and vigilance behaviour and less exploration than did the presence of a control object. However, the dog elicited significantly more of this fear-related behaviour, and significantly larger adrenocortical responses than did the human. Sheep also expressed a clear preference for a human shaking a rattle over a barking dog in the Y maze test and exhibited larger adrenocortical responses to the dog than to the human in the Y maze facility. The UWA flocks differed in their expression of locomotor and vocal activity; MA sheep were more active/vocal than the other flocks, not only in the presence of the human but also with the box or dog. MA sheep expressed less avoidance and vigilance and more exploration than the other flocks in the presence of the human and exhibited significantly lower plasma cortisol concentrations than LA sheep after exposure to the human (10-min sample). However, there were no inter-flock differences in fear-related behaviour or adrenocortical responses when the flocks were presented with the box or dog. The results do not support the notion that the UWA flocks have been selected for differences in a consistent predisposition to react fearfully. The adrenocortical responses measured in these studies were only moderate in magnitude and duration, with peak plasma cortisol concentrations 2-3 times higher than pre-treatment values, and all concentrations returning to pre-treatment levels within one hour of the start of treatment. If these observations are confirmed in practical situations, the presence of humans and dogs during routine handling should cause little concern on the basis of animal welfare. However, limiting the presence of dogs in certain situations (e.g. before slaughter) may reduce stress in domestic sheep. Significant methodological developments in this research include the use of multivariate statistical techniques to analyze arena behaviour, the concurrent measurement of adrenocortical and behavioural responses in the arena and Y maze tests, and the explicit testing of the effects of individual lateral biases on choice behaviour in a Y maze test. Future studies should measure sheep stress responses to the presence of humans and dogs in practical situations.

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Pitta, Dipti Wilhelmina. "Effects of willow (Salix spp.) browse upon ewe reproduction and rumen microbiology under drought feeding conditions : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palmerston North, New Zealand." 2007. http://hdl.handle.net/10179/1391.

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A series of grazing experiments were conducted in the summer/autumn of 2003 and 2004 at Massey University's Riverside dryland farm near Masterton in Wairarapa on the East Coast of NZ, to study the effects of grazing willow fodder blocks (6,000 stems/ha) upon the production and reproductive performance of ewes relative to ewes grazing drought pastures. Drought pastures were simulated in this study and included short drought pasture and long drought pasture. Pasture with a low pre-grazing mass of approximately 1500 kg OM/ha, a dead matter content of >50 % and a sward height of 5-7 cm was defined as short drought pasture typical of drought conditions. Long drought pasture was similar to pasture growing in the willow fodder blocks, with a pre-grazing pasture mass of >4000 kg OM/ha, a sward height of > 30cm and a dead matter content of 30-60 % . Willow fodder blocks were established on low-lying wet, marshy areas of the farm that had very low or zero productivity in the undeveloped state. Pasture development in the fodder blocks was noticed with the growth of unsown grasses and legumes, as the areas dried up following the planting of willow stakes, due to evapotranspiration from the trees. Forage in the willow fodder blocks included both trees and pasture that was grown under the trees. The nutritive value of short drought pasture was low with an ME of 8 MJ/kg O M ; long drought pasture ranged between 8- 1 0 MJ ME/kg DM; willow pasture contained 8 MJ M Elkg DM in 2003 and 1 0 MJ ME/kg OM in 2004. The nutritive value of edible willow tree (<5 mm diameter) was superior to drought pasture with an ME of > 10 MJ/kg OM. The concentrations of the secondary compounds such as condensed tannins (CT ; 30- 40 glkg OM) and phenolicglycosides ( PG ; 1 5-35 g/kg DM) were higher in willow trees compared to their concentrations (CT ; 2-3 g/kg DM) and (PG; 2-9 g/kg OM) in control drought pastures. Experiments involving short drought pasture, long drought pasture and willow fodder blocks as treatment groups were grazed by ewes for 10 weeks in regular breaks from mid February to early May. Ewes were mated during this period and were joined together after mating and grazed on normal pasture until weaning. Live weight (LW) change and body condition score (BCS) were recorded throughout the experiments, whilst reproductive performance of ewes was measured as the number of lambs recorded at ultrasound pregnancy scanning, lambing, docking and weaning. Measurements on wool production were also recorded at weaning. In 2003, experimental ewes grazed control drought pastures (short and long) and willow fodder blocks (restricted and full access) as treatment groups (n= 1 00 ewes/group; Chapter 2). Ewes grazing short drought pasture had an allowance of 0.8 k g DM/ewe/d whilst ewes with restricted access had an allowance of 0.8 kg DM/ewe/d from drought pasture and 0 .4 kg OM/ewe/d from willow fodder blocks. Ewes in full access treatment group had no access to pasture but were confined to willow fodder blocks at an allowance of 2.0 kg OM/ewe/d, which was the same allowance given to long drought pasture ewes. Ewes grazing short drought pasture lost weight at approximately 1 00g/d and recorded a low reproductive rate (90 lambs weaned/100 ewes mated) with a high proportion of single lamb births. Live weight loss was significantly reduced to 40 g/d in ewes grazing willow fodder blocks (full access) with a 20% units increase in reproductive rate due to more multiple births (P <0. 05). Ewes grazing long drought pasture performed intermediate to ewes with full access to fodder blocks and ewes grazing short drought pasture, whilst ewes with restricted access performed similar to ewes grazing short drought pasture. In 2004 (Chapter 3), the restricted access to willow fodder blocks treatment was eliminated from the study and the number of ewes was increased to 165 ewes per treatment group. Performance of ewes grazing short drought pasture was similar to that of ewes grazing short drought pasture in 2003 , with ewes loosing live weight (40g/d) and a low reproductive rate (90 lambs weaned/l00 ewes mated) whilst ewes grazing long drought pasture gained L W (54 g/d) and had a higher reproductive rate (P<0.05). Ewes grazing willow fodder blocks performed better than ewes grazing short drought pasture by maintaining L W and their reproductive rate was intermediate to ewes grazing short and long drought pasture. In 2005, a short grazing trial with rumen fistulated sheep was conducted to study the effect of supplementing willow to ewes grazing drought pastures upon plasma amino acid concentrations (Chapter 4) and upon the microbiology of the rumen (Chapter 5 and 6). Grazing occurred during summer/autumn for 10 weeks with two treatment groups; control (short drought pasture; n=7) at an allowance of 0.8 kg DM/ewe/d and ewes grazing short drought pasture at 0.8 kg DM/ewe/d plus a supplement of fresh willow at 1.4 kg fresh willow/ewe/d (n=7) . Blood samples for the quantification of plasma amino acids were collected at week 5 and 10, with L W and BCS measured at fortnightly intervals. Short drought pasture in this experiment had a low pasture mass (2000 k g DM/ha) and a low nutritive value (8 MJ/kg DM), whilst willow had a higher M E of 10 MJ/kg OM. Both groups of ewes lost live weight at the rate of 50 g/d. Plasma concentration of 3 methylhistidine (3-MTH; 88 vs 127μ mole/L) at week 5 and non essential amino acids (NEAA; 1082 vs 1417μ mo1e/L) at week 5 and ( 1155 vs 1324 μ mole/L) at week 10, were substantially lower (P<0 .05) in w illow supplemented ewes than control ewes. It was concluded that the increased reproductive rate from willow supplementation in ewes grazing drought pasture might be partly explained by reduced body protein catabolism, besides also increasing plasma branched chain amino acids CBCAA) and essential amino acids (EAA) concentrations. To investigate the effects of willow supplementation on rumen microbes, rumen samples were collected during the 2005 experiment with fistulated ewes over a 10 week period. The study involved the use of a molecular technique ( Chapter 5), denaturing gradient gel electrophoresis (DGGE), to compare the rumen microbial populations between the control and supplemented ewes and a cultivation technique (Chapter 6) to study the effect on rumen bacteria of ewes grazing drought pastures with and with out willow supplementation. DGGE analysis of the V3 region of 16S ribosomal RNA genes in DNA extracted from samples of rumen contents taken fortnightly over a 10 week feeding period showed a distinct difference in banding patterns between treatment groups which progressively developed over time, showing rumen microbial adaptation to willow supplementation. However, phylogenetic analysis of the DNA sequences retrieved from the DGGE bands from willow-supplemented and control ewes did not cluster by treatment group. It was deduced that willow supplementation induced a change in rumen bacterial populations through selecting sub-populations of organisms already present in the rumen. The changes in the rumen bacterial populations is attributed to the ability of these bacteria to metabolise secondary compounds in willow such as phenolicglycosides and flavanoid monomers and their ability to resist the inhibitory effects of condensed tannins. The cultivation study involved enumeration, isolation and purification of bacterial colonies on Complete Carbohydrate, Salicin, Xylan, Cellulose and Willow media followed by full characterisation of a representative set of pure bacterial cultures. Total bacterial counts on the above media at week 5 and week 10 were generally lower in willow-supplemented ewes compared to control ewes and the 16S rRNA gene sequences of the majority of iso lates characterised from both Salicin and Xylan media, were most closely related to species from the Pseudobutyrivibrio genus. Isolates from Willow medium clustered as two distinct groups. One group (mostly isolated from control ewes) was made up of mainly of organisms not usually associated with the rumen and probably represent non-resident organisms that are passing through the rumen. The other group of bacteria were mainly retrieved from willow-supplemented ewes and were most closely related to species of the Ofsenella genus. Compared to bacteria isolated on Salicin and Xylan media, isolates on Willow medium showed little ability to ferment various carbohydrates or trypticase (hydrolysed protein) but were able to utilise secondary compounds from willow. It was concluded that willow fodder blocks are useful sources of supplementary fodder for mating ewes during drought situations. Both the field and m icrobiological studies showed adaptation to the willow supplementary diet, including the detection of Olsenelfa-like bacteria for the first time in the rumen. It is suggested that the principal purpose of the rumen investigation is the degradation of secondary compounds present in willow.

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25

Schreurs, Nicola Maria. "Effect of condensed tannin and fresh forage diets on the formation of indole and skatole in the rumen and on the pastoral odour and flavour of sheep meat : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palmerston North, New Zealand." 2006. http://hdl.handle.net/10179/1512.

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Flavour is a factor that has a large influence on meat quality. Pastoral flavour that results from the grazing of pasture is an undesirable characteristic of meat flavour for consumers more accustomed to meat produced by grain and concentrate feeding systems. In New Zealand there is a reliance on grazing systems for sheep meat production, however the resulting meat flavour is one factor that impedes the increase of sheep meat exports to discerning markets. Correlation of chemical analyses to sensory evaluations of sheep meat has identified that a high concentration of indole and skatole in the fat is associated with pastoral flavours. Indole and skatole are formed in the rumen from the microbial fermentation of tryptophan. New Zealand pasture is high in protein, which is both highly soluble and rapidly degraded in the rumen. As such, pasture diets provide a rich and ready source of tryptophan for the formation of indole and skatole in the rumen. Condensed tannins are known to slow the degradation of protein in the rumen. Therefore, one of the objectives of this study was to establish if dietary condensed tannin can reduce the ruminal biogenesis of indole and skatole and consequently, ameliorate pastoral flavour in sheep meat. White clover usually comprises up to 30% of the botanical composition of pastures in New Zealand, is highly degradable in the rumen and likely to result in a high availability of tryptophan in the rumen for conversion to indole and skatole. Therefore, another objective of this study was to determine if feeding white clover gave a significant increase in the formation of indole and skatole in the rumen compared to perennial ryegrass and if this has an effect on pastoral flavour in meat. These hypotheses were tested using a series of in vitro rumen fermentations that incorporated the use of fresh forages (Chapter 3 and 6). In vivo experiments were utilised to assess rumen formation of indole and skatole with different forages (Chapter 4) and to assess effects of dietary condensed tannin (CT; Chapter 5 and 7). Meat and fat samples from lambs used in Chapters 5 and 7 underwent sensory evaluation to determine if forage or CT treatments were having an effect on the fat odour or meat flavour. From the in vitro and in vivo experiments of (Chapter 3, 4, 6 and 7) it was calculated that the formation of indole and skatole with perennial ryegrass is generally only 6-41% of that formed with white clover. A higher concentration of indole and skatole was also observed in the blood plasma of lambs that were fed white clover compared to those that were fed perennial ryegrass (Chapter 7) and white clover gave an overall more intense flavour in the meat. Comparison of forages fermented in vitro (Chapter 3) showed that with forage legumes of a higher CT concentration, such as Lotus pedunculatus (98 gCT kg-1 DM) and Dorycnium rectum (122 gCT kg-1 DM), the indole and skatole formed was only 7-21% of that formed with white clover. With forages of an intermediate CT concentration such as sulla (Hedysarum coronarium) and Lotus corniculatus the indole and skatole concentration formed was 53-68% of that of white clover. From in vitro rumen fermentation of mixtures of white clover and Lotus pedunculatus it was concluded that the CT in Lotus pedunculatus was not reacting with the protein in white clover. Fermentation of fresh white clover in the presence of an increasing concentration of added CT extract showed that at a higher CT, indole and skatole formation were reduced to low levels. It was inferred that this was due to optimal protein binding and the availability of free condensed tannin to bind other sources of protein, including rumen microbes. However, in vivo dosing with a CT extract resulted in only a small reduction in rumen indole and skatole concentration. This indicated that when CT was dosed into the in vivo rumen of lambs fed fresh forages the CT probably passed from the rumen before adequate protein release from the forage had taken place. Thus, in the grazing situation it will be optimal to provide CT in planta to maximise protein binding and this, in combination with the high CT concentration needed (approximately 80 gCT kg-1 DM), makes Lotus pedunculatus or Dorycnium rectum the prime candidates for further grazing trials into pastoral flavour amelioration using CT forages. Grazing Lotus corniculatus (40 gCT kg-1 DM) in a field experiment resulted in a lower rumen and blood plasma and fat concentration of indole and skatole in comparison to the grazing of perennial ryegrass/white clover pasture. However, a change in the pastoral odour of the fat was not perceived by the sensory panel when comparing fat samples from lambs that had grazed Lotus corniculatus and perennial ryegrass/white clover pasture. The concentration of skatole in the body fat was less variable in the lambs that had grazed Lotus corniculatus and resulted in no lambs with a high outlying concentration (>100 ng g-1) of indole and skatole. This finding holds some potential for reducing pastoral flavour for consumers sensitive to high indole and skatole concentration in the fat. When condensed tannin was dosed to lambs that were fed white clover or perennial ryegrass in the form of a grape seed extract the intermittent supply of CT slightly reduced indole and skatole formation in the rumen and reduced the plasma concentration of indole and skatole. Flavour assessment of meat from the lambs fed white clover or perennial ryegrass with or without CT suggested that CT reduced the intensity of pastoral flavours. However, there were minimal effects on indole and skatole concentration in the body fat. It was possible that other pastoral flavour related compounds derived from the degradation of amino acids, in addition to indole and skatole that were measured, were having an effect on the meat flavour. It was concluded that dietary condensed tannin is able to reduce the formation of indole and skatole in the rumen and can alter the sensory attributes of sheep meat including reducing pastoral flavours. A higher CT concentration present within the forage plant (approximately 80 g kg-1 DM) will be best to minimise indole and skatole formation in the rumen and reduce pastoral flavours in the meat. Further research is required to confirm this in the grazing situation. Feeding white clover results in a greater rumen biogenesis of indole and skatole compared to perennial ryegrass and therefore, may be the primary contributor to pastoral flavours when ruminants graze conventional pastures. Further research is required to evaluate the flavour attributes that result from feeding white clover to meat producing ruminants in the New Zealand grazing situation.

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Corner, Rene Anne. "Exposure of ewes to stressors in mid- and late- pregnancy : postnatal effects on the ewe and lamb : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palmerston North, New Zealand." 2007. http://hdl.handle.net/10179/763.

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This thesis set out to examine the effect of maternal undernutrition and exposure to stressors between pregnancy day 50 to 100 and 100 to 147 of pregnancy on the ewe and her lamb. The long-term effects of these stressors during pregnancy on lamb growth, plasma cortisol response to a stressor, metabolism, behaviour and future reproductive success were examined. Mid-pregnancy shearing The component of mid-pregnancy shearing that causes the increase in lamb birth weight is unknown. It was hypothesised that the increase in lamb birth weight was due to the stress response of the ewe to shearing. This work examined the effect of a range of stressors at approximately day 80 of pregnancy. These stressors included yarding, crutching and sham-shearing that may be components of the shearing procedure that produce a stress response. In addition, repeated stressors between day 74 and 106 of pregnancy including isolation, sham-shearing and exogenous cortisol injection were used to examine the role of a longer-term stress response on lamb birth weight. Mid-pregnancy shearing has consistently resulted in an increase in lamb birth weight, however all the other stressors investigated had no effect. Therefore, the hormonal stress response of ewes to shearing was unlikely to be the cause of the increase in lamb birth weight. Mid-pregnancy shearing also resulted in minor changes in ewe and lamb behaviour 12 to 24 h after birth. Shearing during pregnancy had no effect on the cortisol response of ram lambs to handling or castration however differences were observed between singleton- and twin-born lambs. Ewe nutrition during pregnancy Ewes mildly undernourished in between days 70 and 107 of pregnancy that were then provided with adequate nutrition between days 108-145 of pregnancy gave birth to lambs with similar birth weights as ewes well-fed during both periods. Therefore the effects of undernutrition on lamb birth weight can be minimised if undernutrition can be limited to the earlier period (day 70-107 and 108-147 of pregnancy). Mild undernutrition in both periods (day 70 – 145 of pregnancy), resulted in lambs that were lighter and that exhibited behaviours associated with a greater ‘drive’ to maintain contact with their dam than lambs born to ewes well-fed during the same period. This suggests that even mild undernutrition in both mid- and late- pregnancy should be avoided. The effect of maternal nutrition and lamb litter size on the behaviour of female offspring was examined at 1 and 2 years of age. Litter size had no effect on ewe behaviour at 1 year of age however at 2 years of age twin-born ewes had higher maternal behaviour scores than triplet-born ewe lambs. Nutrition of the maternal grand dam during pregnancy had only a minor effect on the behaviour of female offspring at 1 year of age and no effect on maternal behaviour at 2 years of age.

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Romera, Alvaro Jorge. "Simulation of cow-calf systems in the Salado Region of Argentina : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science, Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand." 2004. http://hdl.handle.net/10179/1736.

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The Salado region of Argentina covers 9.5 million ha, is located in the centre-East of the Buenos Aires Province, and concentrates about 6.9 million cattle. Cow-calf systems are predominant in the area. A simulation model was developed with the purpose of assisting in the design and evaluation of cow-calf systems in the Salado Region. The model was designed to produce long term simulations of the dynamic interactions between herd structure, climatic variation and farm management over periods of several decades using daily weather data, real or simulated. Existing models were used to describe soil, pasture and animal components of the farm, linked with management actions in a dynamic framework. The model was driven by decision rules entered by the user, which allowed the representation of management options that respond to changing farm conditions according to a predetermined policy. An object-oriented approach (OOA) was used in the design and implementation of the model. In the OOA, objects in the real world (e.g. cows, paddocks) are represented as objects in the computer program. The simulation of individual cows and individual paddocks made it possible to distribute feed resources flexibly among animals and provided many other points of flexibility in management strategies. The management strategies simulated in trying to improve cow-calf systems in the Salado region were based on Reserva 6, an experimental cow-calf farm located at the INTA-Balcarce Experimental Station. Every spring-summer, 30% of the area is devoted to make low quality hay (by cutting at high herbage mass), most of which is destined to provide maintenance feed for pregnant adult cows in winter. Cows are kept on a small paddock from weaning (March) to calving (August-September), during which time they receive 6-9kg DM of hay per day. A set of decision rules was developed to represent (on a 100ha farm) the management applied in Reserva 6 and, using this as a base system, a series of simulation experiments was conducted. Firstly, three preliminary experiments, aimed at gaining insight into the system and testing the model, were carried out. In the first of these, the effect of delaying the breeding season 15 and 30 days was analysed. The model was run over 30 consecutive years using a real weather sequence, 1970-2000, from INTA-Balcarce, for each scenario. It was found that, when the appropriate management variables (i.e. weaning and sale dates) were adjusted accordingly, changing the calving period had little effect on the productivity of a cow-calf system. In the second experiment, the dynamic consequences of three different heifer replacement policies on the production outcomes of the system were explored. The policies produced different patterns of oscillations in key farm outputs as a result of periodic behaviour in the age structure of the herd, and the differences between strategies were shown to be dependent on the environmental variability being simulated. The third experiment analysed different policies for hay use during the autumn-winter period, including a control strategy in which no hay was harvested or used. The results suggested that, provided hay was utilized on the farm, the pattern of use did not make much difference to liveweight production. Secondly, the long term performance, in terms of annual liveweight sold, of a range of hay quantity-quality combinations was compared. Each policy was simulated across a range of cow numbers (170 to 350, cows plus heifers in a 100ha farm) and was replicated 20 times. Each replication consisted of 50 years of random weather sampled from the real sequence (1970-2000). The benefit of using hay and the contrasts between the effects of different haymaking strategies on animal outputs increased as the cow numbers increased. The long term analysis suggested that the liveweight production of cow-calf farms, under a calendar-based haymaking policy like that followed in Reserva 6, would be maximized by harvesting 40-50% (but not more) of the total farm area and aiming to harvest hay at medium herbage mass (therefore medium quality). Therefore, the policy currently followed in Reserva 6 of allocating 30% of the farm to haymaking could be considered as conservative, and its productivity might be increased by making hay at lower herbage mass. Thirdly, the possible advantages of incorporating flexibility into the haymaking policy used in Reserva 6 were evaluated using the same experimental design. The results indicated that controlling haymaking in a flexible fashion, basing the decisions of closing, releasing and cutting paddocks on a simple pasture budget, would give the system productive advantages (i.e. increases in productivity and reductions in variability) in relation to a calendar-based approach. Using a flexible haymaking policy allows the manager to make more hay than required for the next winter, providing a buffer for the system. A flexible haymaking policy permitted significantly greater levels of herbage utilization by making large amounts of hay without negative effects on the carrying capacity of the system. A preliminary analysis of risk and costs highlighted major advantages in using hay in cow-calf systems, especially when a flexible approach to haymaking is implemented.

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Khan, Md Kabirul Islam. "Development of models for the genetic improvement of dairy cattle under cooperative dairying conditions in Bangladesh : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in animal Breeding and Genetics at Massey University, Palmerston North, New Zealand." 2009. http://hdl.handle.net/10179/1378.

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The aim of this thesis is to enquire into genetic approaches for improving milk yield from dairy cattle in order to overcome the milk shortage in Bangladesh. Survey work on the dairy industry was carried out to reveal its current status. The collected data of different genotypes (Pabna cattle, Australian-Friesian-Sahiwal Pabna, Holstein Pabna, Jersey Pabna, and Sahiwal Pabna) from 1999 to 2001, and in two seasons, were used to predict model parameters, fit-statistics and total lactation yields, by fitting ten lactation curve models. Best fitting model(s) were chosen on the basis of fit-statistics. The input parameters from best fiting model(s) were used for: developing a deterministic model; estimating the profitability of individual cows; estimating whole farm profitability; and for developing a profit function to estimate the economic values of traits in breeding objectives. The individual cow performances for different traits were stochastically simulated in respect of additive genetic, permanent and temporary error, herd and age effects, and mendelian sampling under progeny and parent-average testing breeding schemes based on three selection objectives applied over on 20 year period. Genetic gains in different traits were calculated from the regression of trait values on the selection index. The estimated lactation curves model parameters, and predicted lactation milk yield were significantly different between breeds, years and seasons. From four fit-statistics values, the CCC value was considered superior, and this value indicated that the Nelder model best represented the test day records. The net annual income for Holstein Pabna cattle was the highest (US$229) and was lowest (US$115) for Pabna cattle, while all other genotypes were intermediate. The economic values (EVs) of milk yield for all genotypes were similar (US$0.32), and due to payment for milk volume only, the EVs of fat and protein were negative. EVs of liveweight, calving intervals and calving rate were negative, but survivability was positive in all genotypes. The parent-average testing selection scheme showed higher genetic gains than progeny testing. The highest (US$15.80) genetic gain was obtained for milk yield when selection was for milk merit only. The study will assist in undertaking a genetic improvement programme for the increase of milk production in Bangladesh and thereby enhance food security. (Key words: Dairy cattle, genetic improvements, models, stochastic).

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29

Yang, Linna. "Phenotypic relationships between milk protein percentage, reproductive performance and body condition score in Irish dairy cattle : a thesis presented in partial fulfilment of the requirements for the degree of Master of Sciences (MSc) at Massey University, Palmerston North, New Zealand." 2009. http://hdl.handle.net/10179/1337.

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A positive phenotypic correlation between milk protein percentage and reproductive performance in dairy cattle, especially during early lactation has been recently reported. The objective of this study was to quantify the relationship between milk protein percentage and different measures of fertility in Irish, seasonal calving, dairy cattle using data from experiments comparing strains of Holstein-Friesian cows under different feeding systems. The relationships between body condition score, milk production and fertility were also investigated. The data used in this study consisted of 584 lactation records over a 5-yr period. Principal component analysis and logistic regression was used to study the relationship between milk protein percentage and fertility performance of the cow. Greater milk protein percentage during the first 60 days post-calving was associated with better reproductive performance. The probability of a cow being submitted in the first 21 days of the breeding season increased with increased milk protein percentage during early lactation. Similarly, the probability of a cow becoming pregnant to its first service or to the whole breeding season also increased. Cows were classified as either high or low milk protein percentage based on their protein percentage over the whole lactation. Cows in the high milk protein group had a 7% greater conception rate compared to cows in the low protein percentage group. In conclusion, cows with higher protein percentage, especially during early lactation are submitted earlier in the breeding season, and have a higher conception rate. Physiologically, the shortage of glucose caused by negative energy balance restricts the synthesis of milk protein in the udder. On the other side, negative energy balance also causes the reduction of IGF-I, LH and oestradiol, which consequently delay the ovarian follicular development and finally reduces fertility. Therefore, there is a biological explanation for the association between milk protein percentage and fertility performance.

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Bermingham, Emma Natasha. "The metabolic cost of an intestinal parasite infection on amino acid kinetics in sheep fed fresh forages: a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palmerston North, New Zealand." 2004. http://hdl.handle.net/10179/1743.

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There is mounting evidence that parasitic infections change nutrient utilisation within the tissues, and that this is responsible for the reduction in animal performance that has been observed. Feeding forages that contain condensed tannins (CT) are thought to alleviate the impact of parasite infection on amino acid (AA) and protein metabolism by improving protein supply post-ruminally. However, there has been no quantification of how nutrients are partitioned in the lamb fed fresh forages during a parasitic infection. Therefore, the objective of this study was to quantify the partitioning of AA between the gastrointestinal tract (GIT), liver and the hind limb tissues (muscle, skin, fat) in lambs during an established parasite infection. It was hypothesised that the feeding of CT would alter the partitioning of AA between the GIT, liver and hind limbs in lambs with an established parasite burden due to the increased availability of dietary AA to the small intestine. This hypothesis was tested in two separate experiments, which had a similar experimental design. In the first experiment (Experiment One; 1999) lambs were fed fresh Lucerne (Medicago sativa; contains no CT). In Experiment Two, which was conducted in 2000, the lambs were fed fresh Sulla (Hedysarum coronarium; 2.2% CT; Experiment Two). One week prior to infection, permanent indwelling catheters were placed in the mesenteric artery, and the mesenteric, portal and hepatic veins and vena cava for blood sampling. Additional permanent catheters were placed in the mesenteric vein (upstream from the sampling catheter) and abdominal aorta for infusion of para-aminohippuric acid (PAH) and indocyanin green (ICG) respectively, to measure plasma flow across the splanchnic tissues (PAH) and the hind limbs (ICG). A permanent Teflon cannula was fitted in the abomasum for the infusion of [1-13C]-valine and [35S]-cysteine (Chapters Five, Six and Seven only) on day 48 post infection to measure valine and cysteine kinetics across the mesenteric-drained viscera (MDV), portal-drained viscera (PDV), liver, total splanchnic tissues (TSP; PDV + liver) and hind limbs. A temporary catheter was inserted into the jugular vein two days before the start of blood sampling for the infusion of deuterium oxide (D2O), and [13C]-sodium bicarbonate and [35S]-sulfate (Chapters Five, Six and Seven only) on day 45 post infection, and [3,4-3H]-valine on day 48 post infection. Lambs were dosed with 6 000 L3 T. colubriformis larvae for 6 d (n=5) or kept as parasite free controls (n=6). Faecal egg production was monitored every second day from day 22 to day 48 post infection and total intestinal worm burdens were determined at slaughter. Blood was continuously collected from the mesenteric, portal and hepatic veins, the mesenteric artery and the vena cava in 2-hour aliquots. Plasma was harvested and AA and metabolite concentrations measured and the specific radioactivity (SRA) and isotopic enrichment (IE) of valine and cysteine were determined. After the completion of blood sampling, but while the [3, 4-3H]-valine infusate was still being administered, the sheep were euthanased by an intravenous overdose of sodium pentobarbitone. Tissue samples were rapidly collected from the sheep in the following order: skin, muscle (biceps femoris), liver, duodenum, ileum, spleen, mesenteric lymph nodes and thymus. Digesta was also sampled from the abomasum and ileum after slaughter in order for the apparent absorption of AA to be determined. The results from Experiment One (Lucerne-fed lambs) suggest that there is no re-partitioning of AA from the posterior hind limbs to the GIT and liver during an established infection- The changes that occurred within the PDV suggests that an established parasitic infection may trigger a localised alteration in AA metabolism and/or protein turnover without significantly changing the metabolism of AA and proteins in tissues peripheral to the TSP tissues and impacting negatively on the growth of the parasitised lambs. In Experiment Two (Sulla-fed lambs) a reduction in feed intake was likely to be the reason for the alterations in the first pass metabolism of AA in the TSP tissues due to the decreased apparent AA absorption by the MDV observed in the parasitised lambs. However, the results from this experiment are in agreement with those from Experiment One confirming that there is no increase in partitioning of AA from the hind limbs to the GIT or liver during an established parasite infection. Although a statistical comparison cannot be made between the data in Experiment One (Lucerne-fed) and Experiment Two (Sulla-fed), it appears that the beneficial effects of feeding CT during a parasitic infection is due to the reduction in larval establishment in the GIT of the lamb, rather than increased AA availability. In conclusion, an established infection imposes no measurable metabolic cost on the lamb, when feed intake is not reduced. When feed intake is reduced, there is no detectable mobilisation of protein from the hind limb. Therefore, localised or other sources of AA and/or energy substrates may be utilised.

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Bryant, Jeremy. "Quantifying genetic variation in environmental sensitivity of New Zealand dairy cattle to apply in the development of a dairy cattle simulation model for pastoral systems : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palmerston North, New Zealand." 2006. http://hdl.handle.net/10179/1588.

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The objectives of this research were firstly, to investigate if dairy cattle genotypes in NZ exhibit genetic variation in environmental sensitivity and to determine if this genetic variation is statistically significant from a genetic evaluation perspective, and secondly, to use genetic information including environmental sensitivity data to simulate dairy cattle responses to changes in nutritional regime and variation in climate. A comprehensive review identified that simulation models either overlook, or do not represent environmental sensitivity information where genotypes and breeds respond differently when exposed to variations in environment. A large dataset of daily and total lactation records (yields of milk, fat and protein) from herds participating in the progeny testing of sires from 1989 to 2002 was obtained to test for differences in the environmental sensitivity of dairy cattle in New Zealand. Production data was matched with environmental data relating to climate, herd size, altitude and herd average production levels (a proxy for feeding level). The statistical analyses applying univariate and bivariate multibreed models to environmental character states identified minimal sire re-ranking between environmental character states as measured by genetic and rank correlations. However, differences in yields of milk, fat and protein between New Zealand Jersey and overseas Holstein Friesian systematically diverged with production level, in herds expected to use different levels of supplements. These results suggest New Zealand Jersey cattle are best suited to a grassland-type environment, and overseas Holstein Friesian cattle are more suited to an intensive-type environment. A phenotypic analysis identified thermal environment (cold and hot conditions) significantly affected the expression of production traits in Holstein Friesian, New Zealand Jersey and Holstein Friesian x New Zealand Jersey cattle. Holstein Friesian dairy cattle were more susceptible to the effects of heat conditions than New Zealand Jersey cattle with yields of milk, and concentrations of fat and protein of the former compromised at a lower value for temperature humidity index. Dairy cattle performance is likely to be compromised by heat more frequently than cold conditions in New Zealand. A simulation model that considers how dairy cow genotypes respond to different environments, incorporating the results presented above, was then developed. An initial estimate of feed intake is used to define cow genetic potential based on estimated breeding values for total yields milk, fat and protein, and environmental sensitivity information. A mammary gland module then predicts daily yields of milk, fat and protein based on the cow's genetic potential after considering her age, stage of lactation, body condition score, nutritional status and thermal environment. Live weight change is also predicted via a body energy stores module, which considers the effect of age, stage of lactation, current body condition score, nutritional status, and an estimated breeding value for body condition score. Feed intake is predicted from the requirements for maintenance, growth and pregnancy, and the genetic drive for yields of milk, fat and protein and body fat change. The predictive ability of the model was tested using information from a prior study with two Holstein Friesian genotypes managed in a pasture-based system. The model simulated to a high degree of accuracy, mean values for yields of milk, fat and protein, and concentrations of fat and protein of each genotype. Various tests identified the major source of error between simulated and observed values were due to a lack of simulated variation. In conclusion, the extent of genetic variation in environmental sensitivity for total lactation yields of milk, fat and protein within the range of New Zealand environments are not sufficient to warrant the formation of separate breeding schemes for distinct environments. However, New Zealand Jersey cattle are best suited to a grassland-type environment, and overseas Holstein Friesian cattle are more suited to an intensive-type environment. Genetic variation in the suitability of different breeds for specific environments existed within breeds. A simulation model was developed that was able to simulate the effect of genotype, environment and genotypic differences in environmental sensitivity on daily cow performance.

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32

Sharma, Rajesh. "The effects of uterine environment upon embryonic, fetal, neonatal and post-natal development and glucose metabolism in sheep : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Palmerston North, New Zealand." 2010. http://hdl.handle.net/10179/1689.

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Studies of humans and domestic animals have shown that there is a linkage between the neonatal and post-natal health of an individual and its uterine environment during gestation. However, very little information exists for sheep and there have been no studies that have directly examined the stage of gestation at which such effects could be introduced to the conceptus. In the present study, pure-breed embryos were transferred within and reciprocally between large (Suffolk: S) and small (Cheviot: C) breeds of sheep to establish different uterine environments; SinS (large control), SinC (restricted environment), CinS (luxurious environment) and CinC (small control) and their effects upon embryonic, fetal, neonatal and post-natal development and glucose metabolism of lambs were examined. By Day 19 of gestation, conceptuses (embryo and trophoblast) developing in a restricted uterine environment (SinC) were smaller (P<0.05) than in control (SinS). The head length of SinC fetuses was smaller (P<0.05) than in SinS fetuses on Day 55 of gestation and SinC lambs were lighter and smaller (P<0.05) than SinS lambs at birth. During subsequent post-natal life, there was no difference (P>0.05) in the growth rate of SinC and SinS lambs. The liveweight and body dimensions of SinC lambs were lower (P<0.05) than SinS lambs until 9 weeks and 12 weeks of age, respectively. Day 19 peri-implantation embryos and trophoblasts that developed in a luxurious environment were bigger than in control (CinC). However, CinS fetal size did not differ (P>0.05) from CinC fetuses by Day 55 of gestation. There was no difference (P>0.05) in the birthweight and body dimensions of lambs born from these two groups. Dimension of the placentas of SinC and SinS or CinS and CinC did not differ (P<0.05) during gestation or at lambing. Concentrations of ovine placental lactogen (oPL), progesterone, insulin-like growth factor-1 (IGF-1), glucose and free fatty acid (FFA) differed between uterine environments. During glucose challenge tests, there were no differences in the concentrations of glucose and insulin, between SinC and SinS female lambs, however, glucose concentrations declined more rapidly (P<0.05) in CinS than CinC female lambs at one year of age. It was concluded that restricted uterine environment affects embryonic, fetal and neonatal development of lambs, and that these effects perpetuates until at least one year of age; but there was no effect upon glucose metabolism. Conversely, a luxurious uterine environment enhances the early development of embryos but had no effects upon subsequent fetal, neonatal and post-natal development; however glucose metabolism of post-natal female lambs was improved. It appears that these effects of uterine environment were mediated through the trophoblast during the early embryonic period and via the placenta during subsequent gestation. oPL, progesterone, IGF-1, glucose and FFA were implicated in feto-maternal dialogue. These results suggest that uterine environment significantly influences the biology of young sheep with possible economic consequences.

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33

Mwendwa, James Musyimi. "Investigating summer and autumn endoparasitism in farmed red deer, effects of weaning date, anthelmintic treatment and forage species : a thesis in partial fulfilment of the requirements for the degree of Masters of Science in Animal Science at Massey University, Palmerston North, New Zealand." 2007. http://hdl.handle.net/10179/837.

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Previous research has shown that even small numbers of lung and gastro-intestinal (GI) nematodes cause sub-clinical infections during autumn and can reduce voluntary feed intake (VFI) and liveweight gain (LWG) post-weaning in farmed deer. However, little is known about the effect of parasitism on growth of young farmed deer prior to weaning (summer and early autumn). At present, the control of deer parasites is largely by anthelmintic treatment. Alternatively, chicory sown as a pure sward has been shown to reduce parasitism and increase post-weaning growth of deer compared with perennial ryegrass-based pasture, although, neither forage plantain nor the inclusion of chicory in a pasture mix have yet been evaluated in this context. In addition, it has been suggested that to achieve a high pregnancy rate early in the mating season, deer calves should be weaned prior to mating to optimise nutrition and body condition of the hinds. At present there is no adequate evidence in the published literature to justify this. Two experiments were conducted in 2005 and 2006 respectively. The first experiment investigated the impact of early or late pre-rut weaning, with and without anthelmintic treatment, on parasitism and growth (LWG g/day) of deer calves during summer and early autumn. Weaning date effects on hind reproductive parameters were also investigated. The second experiment was a preliminary investigation to compare the effect of grazing permanent perennial ryegrass pasture (Lolium perenne) with chicory (Cichorium intybus) narrow-leaved plantain (Plantago lanceolata) and pasture mixes based on short-rotation tetraploid ryegrass (TSR-mix; nil endophyte) or long-rotation tetraploid ryegrass (TLR-mix; low endophyte), with both mixes sown with the same clover (white and red) and chicory, on post-weaning growth and endoparasitism of weaned farmed red deer calves in autumn. In 2005, seventy-six deer calves were randomly allocated in a 2x2 factorial design, involving sex, genotype, weaning date (February 17 or March 17), treatment with either topical moxidectin (0.5mg/kg) on January 14 and February 25 or no anthelmintic treatment. Liveweight gain, faecal gastrointestinal egg counts (FEC) and lungworm larval counts (FLC), haematological parameters and serum proteins concentrations (i.e., total protein, albumin and globulin) of calves were measured. Mixed-age adult hinds (64) were used to investigate the effect of weaning date on internal parasitism, conception date and pregnancy rate determined by ultrasound scanning. These hinds were not given anthelmintic treatment, but FLC and FEC were determined on January 12, February 17, March 17, March 31 and May 4. All deer rotationally grazed permanent perennial ryegrass-based pasture (Lolium perenne) together until weaning at which point calves were removed to separate but similar pasture. Calves weaned in March had a higher LWG to March 31 than those weaned in February (P<0.0001). Faecal larval count in treated calves was zero, but FEC remained similar to the untreated control calves, regardless of when they had been treated (average 136 epg, range 0-600 epg in mid February and average 92, range 0-350 epg at the end of March). Treated calves had higher serum albumin, and lower serum globulin concentrations than the untreated control group (albumin, 36.2 ± 0.3 vs 35.2 ± 0.3g/L; P<0.001; globulin, 23.9 ± 0.4 vs 25.5 ± 0.4g/L; P<0.005). In hinds, FLC averaged 5 lpg (range 0 – 122) and FEC averaged 26 (range 0- 200) with no significant relationship between weaning date and either FLC or FEC. No effect of weaning date on conception rate or date was observed. This study showed that pre-rut weaning date, (although confounded by weaning process management) and sub-clinical parasitism during summer and early autumn may influence LWG in young farmed deer. The failure of moxidectin to reduce FEC to zero raises the question of the efficacy of this macrocyclic lactone anthelmintic against GI nematodes in farmed deer and/or emergence of farmed deer GI nematode resistance. Potential diagnostic parameters such as serum albumin concentration, which was reduced in untreated control deer, warrant further investigation for clinical diagnostic use in farmed deer. The study also highlighted the need for further research to demonstrate the advantages or disadvantages of pre-rut weaning on growth of deer calves and hind reproduction. The 2006 grazing experiment investigated the effect of pasture species grazed on post-weaning growth and endoparasitism of farmed red deer from 3- 6 months of age. Ninety-five red deer calves were randomly allocated to five groups based on sex, LW, FEC and FLC. These calves rotationally grazed either a permanent pasture based on perennial ryegrass, chicory, narrow-leaved plantain, or one of two pasture mixes based on either a short-rotation tetraploid ryegrass (TSR-mix) or long-rotation tetraploid ryegrass (TLR-mix). Both mixes included the same white clover, red clover and chicory. All deer were initially treated with an anthelmintic (albendazole), with subsequent trigger treatment withheld until weight loss or clinical parasitism was observed. Anthelmintic trigger treatment (albendazole) was given on an individual animal basis. The anthelmintic trigger treatment and LWG data in this study suggest that plantain, TLR- and TSR-mixes and chicory may all have a role in aiding control of endoparasitism in young growing deer in autumn in deer production systems based on permanent perennial ryegrass-based pasture with low anthelmintic input. However, any potential effects of forage feeding value and anti-parasitic plant compounds of chicory, plantain and pasture-forage mixes on parasitism and growth could not be separated in this study. It is therefore acknowledged that these observations are preliminary and based on a design intended only to establish whether further replicated studies are warranted, particularly with plantain and pasture mixes. However, the study has shown that pasture species, either sown as a pure crop or in a pasture mix can influence LWG, resilience to internal parasitism and requirement for anthelmintic use in young farmed deer. Data from the first experiment (2005) has shown that pre-rut weaning date and sub-clinical parasitism during summer and early autumn can influence LWG in young farmed deer while the trigger treatment and LWG data from the second experiment (2006), suggest that plantain, TLR-and TSR-mixes and chicory may have a role in aiding control of internal parasitism in young deer in autumn. The outcome of the second experiment has application to deer production systems with low anthelmintic input. Serum protein and haematological parameters investigated in both studies demonstrated the need for further research to establish diagnostic markers for both sub-clinical and clinical internal parasitism in farmed young deer.

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34

McWilliam, Eileen Lee Hafner. "The effect of poplar (Populus spp.) and willow (Salix spp.) supplementation on the reproductive performance of ewes grazing low quality drought pasture during mating : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science in the Institute of Veterinary Animal and Biomedical Science, Massey University." 2004. http://hdl.handle.net/10179/1741.

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A series of grazing experiments was conducted, in the summer/autumn of 2001, 2002 and 2003, to investigate the effects of poplar (Populus spp.) and/or willow (Salix spp.) supplementation, during mating, on ewe production and reproduction when grazing drought pasture. Each experiment involved a rotational grazing system with 300 mixed-age Romney ewes, divided into three groups of 100 ewes each. In each year, all ewes were offered low quality simulated drought pasture, containing more than 60% dead matter, at an allowance sufficient to provide a potential desired intake of 0.70 kg dry matter (DM)/day, for periods of 9 to 12 weeks, including two mating cycles. Mean pre-and post-grazing pasture masses averaged over the three years were 1100 and 600 kg DM/ha. The pasture consumed in all years was typical of pasture available to grazing livestock in a drought; it was high in neutral detergent fibre (NDF; approximately 600 g/kg DM), low in organic matter digestibility (OMD, approximately 0.52) and metabolisable energy (ME; approximately 7.5 MJ/kg DM) and contained approximately 20 g nitrogen (N)/kg DM. The supplementary poplar and willow diets were always superior to drought pasture consumed by the ewes, being higher in OMD (approximately 0.67), ME (approximately 10 MJ/kg DM) and total N (approximately 26 g/kg DM) and lower in NDF (approximately 383 g/kg DM). Tree fodder diets also contained substantial concentrations of the secondary compounds condensed tannin (CT; range 7 to 52 g/kg DM), salicin (approximately 2 g/kg DM) and other phenolic glycosides (approximately 21 g/kg DM), with willow (27 to 52 g/kg DM) containing greater concentrations of CT compared with poplar (7 to 19 g/kg DM). Mean diameter of the tree fodder stem consumed during the series of experiments was approximately 7 mm for poplar and 4 mm for willow with the diameter increasing over the experimental periods in four cases out of live (P<0.05). After the supplementation period, the three groups were joined together and grazed on perennial ryegrass/white clover pasture until the conclusion of each experiment at weaning. In all years, the effect of poplar and/or willow supplementation on ewe live weight (LW) and body condition score (BCS) change; the proportion of lambs (reproductive rate) at pregnancy scanning, lambing, docking and weaning; and wool production and staple length from ewe fleeces with approximately 11 months growth, were measured. Experiment 1 was designed to determine how much poplar fodder needed to be fed to increase ewe production and reproduction over a 71-day supplementation period. The experiment involved a high supplementation group, offered 1.5 kg fresh poplar/ewe/day; a low supplementation group, offered 0.75 kg fresh poplar/ewe/day; and a control group that was offered no tree fodder. Ewes in the high and low treatments lost less LW (-67 and -71 vs. -82 g/day; P<0.05) and BCS (-0.78 and -1.27 vs. -1.31 units; P<0.05) compared with unsupplemented ewes. Reproductive rate was relatively low in the control group (121 lambs born/100 ewes mated), with poplar supplementation increasing ewe reproductive rate by approximately 20% units (P<0.05) and 30% units (P<0.001) for the low and high treatment groups, respectively, at scanning, lambing, docking and weaning. The increase in reproductive rate in supplemented ewes was due to increases in both conception rate (number of ewes pregnant/100 ewes mated) and fecundity (number of lambs born/100 ewes mated). Experiment 2 was designed to determine if production and reproduction varied between ewes fed poplar versus willow at the same rate of supplementation, 1.4 kg fresh forage/ewe/day, for 87 days. Again, reproductive rate was relatively low in the control group (133 lambs born/100 ewes mated), with willow supplementation reducing L.W loss (-86 g/day vs. -103 g/day; P<0.01) and increasing reproductive rate by 15%, 17% 21% and 20% units at ultrasound scanning (P=0.097), lambing (P=0.087), docking (P<0.05) and weaning (P=0.058), respectively. The increase in reproductive rate was due to an increase in fecundity; supplementation did not affect conception rate in this experiment. Unlike the previous experiment, poplar supplementation showed no effect on reproductive rate, despite the increase in DM intake and the apparent reduction in LW loss of 9 g/day (P-0.11). It is likely that severe contamination of the poplar fodder with Melampsora larici-populina, or poplar leaf rust, confounded the results. Building on the results of the first two grazing trials, the next step was to determine the period (days) of tree fodder supplementation necessary to achieve a response in reproductive rate. Experiment 3 involved ewes fed 1.3 kg fresh willow/ewe/day for a 'long' period, 63 days including 6 weeks of mating, and a 'short' period, 31 days including 3 weeks of mating. The mating period commenced on the same day for all groups and lasted for 6 weeks. Willow supplementation for 63 days reduced ewe LW loss (-96 g/day vs. -147 g/day; P<0.05) and BCS (-0.79 VS. -1.09; P<0.05) loss, compared with unsupplemented ewes; however, it did not increase reproductive rate at scanning and lambing. The lack of response in willow-supplemented ewes was likely to be due to toxic concentrations of zearalenone (1.5 mg/kg DM), an oestrogenic mycotoxin, in the drought pasture during mating, which confounded the results by negating any potential benefits due to increased nutrient intakes. Willow supplementation for 63 days did increase reproductive rate at weaning by 13% units, due to a 9% unit (P<0.05) reduction in post-natal lamb mortality, from 17.1 to 8.4%. Supplementation for 31 days did not appear to influence ewe reproduction and production parameters. Overall, the rate of LW loss was greater in Experiment 3 compared with the first two experiments. Seven indoor in vivo digestibility experiments were conducted at the following times; early April 2001 (poplar), February, March and April 2002 (all poplar), and December, March and April 2003 (all willow). Each 14-day trial involved 6 male cryptorchid lambs, individually fed in metabolism cages. The experiments showed that the digestibility of poplar and willow tree fodder declined from late spring to autumn (P<0.05), but that the decline was much smaller than the decline in digestibility of grass-based pastures in New Zealand over the same time period. The experiments also showed that mean ME and digestibilities were generally higher for willow than for poplar. The seven in vivo digestibility coefficients were then used to develop a standard curve for in vitro prediction of in vivo digestibility, this standard was used to analyse all unknown tree fodder samples from the three grazing experiments. Results from the three grazing experiments showed that supplementing ewes grazing drought pasture during mating with poplar and willow tree fodder consistently increased DM intake by 0.25 to 0.33 kg DM/ewe/day for ewes offered 1.3 to 1.5 kg fresh willow or poplar each day and increased calculated total DM intakes from 0.67 to 1.03 kg DM/ewe/day in Experiment 1, from 0.59 to 0.86 kg DM/ewe/day in Experiment 2 and from 0.47 to 0.75 kg DM/ewe/day in Experiment 3. Supplementation also consistently reduced LW loss and loss in BCS and substantially increased lambing rate through increased conception rate and fecundity and reduced post-natal lamb mortality. The effects on LW and BCS gradually declined in the post-treatment period and were no longer evident by commencement of lambing. There was no effect of supplementation on wool production or staple length in any of the experiments. One of the unexpected results of the experiments was an average 34% reduction in post-natal lamb mortality over three years, due to willow/poplar supplementation of ewes during mating. Initial results showed that despite significant increases in fecundity in supplemented ewes in 2001 and 2002, post-natal lamb mortality was not increased. This result, combined with a statistically significant reduction in lamb mortality in Experiment 3 (P<0.05), in the absence of any differences in fecundity between the groups, suggested that tree fodder supplementation during mating may have reduced lamb mortality in all three years, but that the effect was masked by the increase in reproductive rate in the first two experiments. Therefore, data from the three field trials were combined and analysed by adjusting all mortality data to equal birth rank and sex; this showed a significant reduction due to supplementation (P<0.05) with no treatment-year interaction. The increase in ewe production and reproduction in supplemented ewes was likely due to increases in nutrient intake, through increased DM, ME and CP intakes, prior to and during mating and to increased outputs of undegradable dietary protein and microbial protein from the rumen, per unit of crude protein consumed, thus increasing amino acid absorption. An increase in ovulation rate of 1.5 % units/MJ of digestible energy consumed (Smith 1985) should result in increases in ovulation rate due to tree fodder supplementation of only 5 and 4% units in 2001 and 2002, respectively; however, the increases in scanning rate were substantially greater at 41 and 16% units. Therefore, it is possible that the majority of the increase in reproductive rate was due to increased essential amino acid absorption, which is consistent with increases found in ewes mated on CT-containing forages such as Lotus corniculatus (Birdsfoot trefoil). Gross margin analyses using actual data from unsupplemented ewes in each of the three grazing trials compared with Riverside Farm's commercial ewes from the same years showed that drought reduced scanning rates by an average of 22.4% and wool production by 20% and that this reduction decreases sheep production income by approximately $14/ewe. Further analysis showed that almost half the cost ($6/ewe) could be recovered by supplementing ewes with tree fodder in a drought. On a whole farm basis this represents $58/hectare cost benefit due to tree fodder supplementation. Fungal contamination was a significant factor in the results obtained in Experiments 2 and 3. In all years, simulated drought pasture contained metabolites of zearalenone and the trichothecenes nivalenol and deoxy-nivalenol, produced by Fusarium fungi, while in Experiment 2 the poplar was severely contaminated with Melampsora larici-populina, or poplar leaf rust. Zearalenone concentrations in pasture were at their greatest in Experiment 3 and increased to over 2 mg/kg DM during the mating period. This may explain the lack of increase in reproductive rate expected in willow-supplemented ewes in Experiment 3, which was a feature of previous experiments; however, it did not explain the much greater loss in ewe LW in Experiment 3. Nivalenol (NIV) and deoxy-nivalenol (DON) are common trichothecene toxins found in New Zealand pasture and were found in pasture samples from all three experiments, however, the concentration in Experiment 3 was three- to four-fold greater than in previous experiments. Reports have suggested that trichothecenes may be partly responsible for the reduced growth of otherwise healthy livestock grazing dry autumn pasture, often referred to as 'ill thrift'. However, based on evidence from dosing experiments, it is unlikely that the quantities of NIV and DON present in pasture in Experiment 3 accounted for all of the greater LW loss seen in this experiment. This suggests that these toxins are likely to be indicators of other more potent fungal toxins, which have a much bigger impact on livestock health and production. It is likely that fungal toxins contribute more to reduced reproduction in breeding ewes and to ill thrift in young stock grazing dry autumn pastures in East Coast regions than is currently acknowledged

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35

Lazzarini, Maria Belen. "Milk production and nitrogen partitioning in dairy cows grazing standard and high sugar perennial ryegrass with and without white clover, during spring and autumn : a thesis presented in partial fulfilment of requirements for the degree of Master of Applied Science in Animal Production at Massey University, Palmerston North, New Zealand." 2010. http://hdl.handle.net/10179/1658.

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Two field grazing experiments were conducted in New Zealand (NZ) in spring (Experiment 1; November 2008) and autumn (Experiment 2; April 2009) to evaluate the effects of feeding a high sugar perennial ryegrass (HSG; cv. AberDart; derived in the United Kingdom; UK) versus a NZ- derived control grass (cv. Impact) on milk production and estimated nitrogen (N) partitioning within the cow. Areas of both ryegrasses were replicated and sown with or without white clover (cl) (HSG+cl, control+cl, HSG and control). A cross-over design with four 10-day periods was used in each experiment, using 15 Friesian cows per treatment per period in Experiment 1 and 5 cows per treatment per period in Experiment 2. Treatment effects upon pasture botanical and chemical composition, cows’ milk yield and composition, and estimated N partitioning were studied. Nitrogen partitioning was calculated using indirect methods. Herbage concentrations of water soluble carbohydrates (WSC) were lower in autumn than in spring whilst crude protein (CP) concentrations were higher in autumn. Organic matter digestibility (OMD) and metabolisable energy (ME) concentration was similar in both seasons. There were no differences in the concentration of CP, WSC and dry matter (DM) among treatments in Experiment 1. The HSG+cl treatment had the lowest concentrations of neutral detergent fibre (NDF, 417 g/kg DM) and the highest content of ME (12.6 MJ/kg DM) and tended to have the lowest sward dead matter content compared with the other three treatments. In Experiment 2 both HSG treatments showed higher concentrations of WSC (15 g/kg DM) compared with the control, both with and without clover; the concentrations of NDF and acid detergent fibre (ADF) were the lowest for both HSG treatments. In Experiment 1, cows grazing treatments with white clover produced more milk (1.6 kg/day) and more milk solids (MS; 0.16 kg/day) than cows grazing pure ryegrass swards ( P< 0.01), with highest milk yields being from cows grazing the HSG+cl treatment (ryegrass cultivar x white clover interaction P<0.05). No differences in milk production were found in Experiment 2. Estimated urinary N excretion (g/day) was similar for all treatments in both seasons, although N intake differed among treatments. The proportion of N intake excreted in urine or secreted in milk was similar for all treatments in both experiments. Nitrogen output (g/day) in milk was the highest for the HSG+cl treatment in Experiment 1 but no differences were found in Experiment 2. Data were combined from both experiments to study the effects of the herbage CP:WSC ratio upon estimated N partitioning between milk and urine. Mean ratios were 0.72 for spring herbage and 2.27 for autumn herbage. As the amount of WSC increased in the diet relative to the amount of CP (thus a lower CP:WSC ratio) there was a significant increase in the amount of milk N secreted per unit of N intake in spring but not in autumn. The breakpoint in the relationship between the herbage CP:WSC ratio and the nitrogen utilisation efficiency for milk production (NUEm) was 1.32, and the NUEm for that breakpoint was 14 g milk N per 100 g N intake. Ratios below this point were associated with improved efficiency of converting pasture N to milk N; ratios above this point were not correlated with changes in N conversion efficiency. It is concluded that the CP:WSC ratio in perennial ryegrass may be important in the partition of absorbed N into milk or urine. A NZ-selected HSG with a lower CP:WSC ratio is likely to have major benefits for pastoral farming in NZ. In order to be effective, a NZ-derived HSG should substantially increase WSC concentration in autumn pasture (from approximately 100 to 200 g/kg DM) whilst reducing CP content simultaneously (from 240 to 190 g/kg DM). The lower structural fibre and higher milk production for the HSG+cl treatment in both experiments suggest that under NZ conditions, best productive responses to HSG may be obtained in management systems that include white clover.

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36

DeNicolo, Gina. "Accelerated and out-of-season lamb production in New Zealand : a thesis submitted in partial fulfilment of the requirements for the degree of Doctor of Philosophy at Massey University, Palmerston North, New Zealand." 2007. http://hdl.handle.net/10179/775.

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The objective of this study was to evaluate ewe and lamb performance in an accelerated lamb production system, and to compare the performance and lamb output between a conventional and an accelerated lamb production system. In the “Conventional” system, ewes were bred in March to lamb in August. The “Accelerated” system was based on the “STAR” system (Lewis et al., 1996), in which there were five breeding periods within each year. In the current experiment these were 14th January, 28th March, 9th June, 21st August and 2nd November. Progesterone was used to synchronise the breeding periods and during the non-breeding season, eCG was used to induce reproductive activity. Lambing began on each of these dates and weaning was 73 days later, coinciding with the next breeding period. The experiment ran over a three-year period beginning with breeding in March 2003 and was complete with the weaning of lambs from the January 2006-bred ewes. This resulted in 15 lambing and breeding periods over the three years in the Accelerated system and three lambing and breeding periods in the Conventional system. Average pregnancy rates were lower in the Accelerated system than in the Conventional system. Lamb growth rates were similar between the two systems, although lamb live weights at weaning were lower in the Accelerated system due to the age of the lambs at weaning (average = 69 vs 96 days). More lambs were born and weaned, resulting in more kilograms of lamb weaned in the Accelerated system relative to the Conventional system over the experimental period (26,200 vs 24,300 kg). Labour input was 35% higher in the Accelerated system, or 13% higher per lamb weaned. Average annual ewe energy requirements were 6% higher in the Accelerated system. Ewe energy requirements per kilogram of lamb weaned was lower (6%) in the Accelerated system due to more breeding and lambing periods per ewe per year. Laparoscopic observation of ewes’ ovaries at each breeding period revealed that most ewes had active ovaries and were therefore capable of successfully producing a viable foetus. In a subsequent experiment, blood samples were collected for analysis of progesterone concentrations from ewes bred during the spring and autumn breeding periods. Observations of data indicated that a small number of ewes conceived and lost their conceptus, or had abnormal corpora lutea. Results suggested that pregnancies were failing due to a lack of an appropriate signal from the embryo to the dam/uterus. Exposing Romney ewes to an artificial lighting regimen was unsuccessful for inducing reproductive activity during spring. In another experiment, melatonin implants administered to Romney ewes in spring and used in conjunction with eCG and progesterone, resulted in 61% more lambs born per ewe treated, compared to eCG and progesterone alone. This result indicated that melatonin implants, used with eCG and progesterone may be a suitable method for improving reproductive performance in sheep bred out of season in New Zealand. Delaying weaning of lambs and breeding lactating ewes can be used to obtain heavier lamb weaning weights in the Accelerated system. Spring-bred ewes had lambs weaned at either 69 days post partum or 90 days post partum. Reproductive performance was similar between the two groups of ewes, and lamb live weights in the later weaned group were heavier when lambs were 90 and 120 days of age. This research has shown that accelerated or out-of-season lamb production is an option for some New Zealand sheep farmers. However, the mechanisms associated with reproductive seasonality and methods of successfully circumventing this seasonality require further attention in order to achieve optimum reproductive performance.

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37

Laca-Vina, Hector. "An application of Malmquist productivity index to compare technological and growth differences between traditional and non-traditional dairy regions in New Zealand : a thesis presented in partial fulfilment of the requirements for the degree of PhD in Agribusiness at Massey University, Palmerston North, New Zealand." 2010. http://hdl.handle.net/10179/1290.

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The NZ dairy industry has adopted an encompassing measure of performance, total factor productivity (TFP), as a target measure to guide on-farm improvements. Dairy farmers pay a levy in order to fund agricultural research and extension. Extension services and R&D will continue to be of critical importance to maintain and improve productivity at the farm level. Consequently, it is in the best interest of the dairy industry to adequately target R&D and extension funds and make the best use of resources. To date, the methodology employed to estimate productivity growth has some shortcomings that seriously hamper the ability of potential users to extract useful information from it. First, productivity growth has been reported as an aggregate for the entire dairy industry. Second, it makes no assumption about the efficiency with which resources are being used. Third, it implicitly assumes that all farms face the same technology. Productivity growth can be achieved either through better (more efficient) use of the technology applied, through the adoption of a new technology (technical progress) or a combination of both. Given that the sources of productivity change—technical progress and technical efficiency change—are fundamentally different phenomena, they are, in turn, influenced by different factors. This distinction is important for policy orientation because different instruments/tools may be required to address them. Furthermore, empirical evidence suggests that a variety of farming systems have emerged as a result of dairy farming geographical expansion. Farm-level panel data were used to estimate the Malmquist productivity change index. This index can provide additional insights since it can be decomposed into two additional components, one that measures changes in technical efficiency (i.e., whether firms are getting closer to the production frontier over time), and one that measures changes in technology (i.e., whether the production frontier is moving outwards over time). Hence, it provides individual (farm) estimates of TFP. Moreover, the methodology applied allows to test whether farms in the two regions considered in this study are operating under the sametechnology. These two regions were the long-established dairy areas of Waikato-Taranaki and the newly developed dairy areas of Canterbury-Southland. Results for farms in Waikato-Taranaki indicate that annual TFP change is modest, ranging from 0.29% per annum to 0.59% per annum. Most importantly, technical progress is the only source of TFP change in all four models. Therefore, it is necessary to encourage investments in new R&D targeted to remove the technological constraints that impede the realisation of further productivity gains in the regions. However, important differences in the estimates of TFP, technical progress and change in technical efficiency between models were found for farms in Canterbury-Southland. Estimates of TFP change ranged from 0.7% per annum to 2.8% per annum. Even though technical progress and change in technical efficiency contributed to total factor productivity growth (TFPG), the latter component was the most important contributor in three of the four models. Moreover, in two models the rate of technical progress was negative (i.e., technical regress). The analyses indicate that dairy farms in Canterbury-Southland were on average 10% more productive than farms in Waikato-Taranaki when farms in both regions faced the frontier. These results were consistent for all the input/output set chosen. Furthermore, the null hypothesis that the two regions do not face the same production technology (i.e., each region has it own production frontier) was accepted irrespective of the input/output set chosen. The rejection of the null hypothesis, that farms in traditional and non-traditional dairy regions were operating under the same underlying technology (and hence face the same production frontier), called for a review of the traditional approach to R&D in one central experimental station, strengthening the need for a local approach through the promotion of networks and synergies with universities and other research institutions.

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Goodwin-Ray, Kathryn Anne. "Pneumonia and pleurisy in sheep : studies of prevalence, risk factors, vaccine efficacy and economic impact : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy at Massey University, Palmerston North, New Zealand." 2006. http://hdl.handle.net/10179/1497.

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The objectives of this thesis were to investigate patterns of lamb pneumonia prevalence of a large sample of New Zealand flocks including an investigation of spatial patterns, to evaluate farm-level risk factors for lamb pneumonia, to determine the efficacy of a commercially available vaccine for the disease and to estimate the likely cost of lamb pneumonia and pleurisy for New Zealand sheep farmers. Data were collected by ASURE NZ Ltd. meat inspectors at processing plants in Canterbury, Manawatu and Gisborne between December 2000 and September 2001. All lambs processed at these plants were scored for pneumonia (scores: 0, <10% or ≥10% lung surface area affected) involving 1,899,556 lambs from 1,719 farms. Pneumonia prevalence was evaluated for spatial patterns at farm level and for hierarchical patterns at lamb, mob and farm levels (Chapter 3). The average pneumonia prevalence in Canterbury, Feilding and Gisborne was 34.2%, 19.1% and 21.4% respectively. Odds ratios of lambs slaughtered between March and May were vastly higher than those slaughtered in other months indicating longer growth periods due to pneumonia. Since pneumonia scores were more variable between mobs within a flock than between flocks, it was concluded that pneumonia scores were poor indicators for the flock pneumonia level due to their lack of repeatability. There was no statistically significant spatial autocorrelation in pneumonia prevalence for any region, hence lamb pneumonia appeared to be largely independent of topographical and geo-climatic factors. A questionnaire-based case-control study was conducted investigating farm-level factors from a sample of farms with either high (case) or zero (control) pneumonia prevalence at slaughter (Chapter 4). Significant risk factors for case farms were: (1) shearing lambs on the day of weaning, (2) breeding ewe replacements on-farm (3) number of lambs sold (an indicator of flock size) and (4) increased percentage of lambs sold late in the season (March to May). Significant protective factors included: (1) set stocking lambs after weaning, (2) injecting lambs with Vitamin B12 at the time of tailing, (3) injecting lambs with Vitamin B12 at weaning. In Canterbury, flocks with Romney ewes and other ewes had a higher risk of pneumonia than those with fine wool type ewes (Merinos, Corriedales or Halfbreds). In a clinical trial, 8,364 lambs from seven commercial sheep farms with a history of lamb pneumonia were vaccinated with Ovipast Plus® or placebo by systematic random allocation within mob and farm. An assessment of the extent of pneumonic lesions was conducted at slaughter and lamb growth rate was monitored through the growth period (Chapter 5). The vaccination trial showed no statistically significant effect of Ovipast® vaccination on the extent of lung lesions at slaughter or ADG of lambs from the first treatment until slaughter. No significant differences were found between isolation rates of Pasteurella spp and patho-histological classifications from pneumonic lung samples of placebo and vaccinated lambs. A spreadsheet-based stochastic model was constructed to estimate the cost of lamb pneumonia and pleurisy to New Zealand farmers. The estimate was based on data of the effect of pneumonia on lamb growth rate, distributions of pneumonia severity, prevalence of moderate to severe pneumonia (≥10% lung surface area) and pleurisy prevalence (Chapter 6). The simulated annual average cost of pneumonia was NZ $28.1 million and that of pleurisy NZ $25.1 million. The combined cost of pneumonia and pleurisy to New Zealand farmers had an average of NZ $53.2 million (95% stochastic interval = $32.4-$78.9 million), or US $31.9 million per annum. This would equate to NZ $2.32 per lamb. In comparison, animal health, shearing expenses and feed expenses cost NZ $2.37, $2.62 and $1.85 per lamb, respectively. This research has demonstrated sub-clinical pneumonia to be a widespread disease in the New Zealand sheep farming population while previous research has focussed on case studies of affected farms. The estimated costs of pneumonia and pleurisy to New Zealand farmers ($53.2 million) highlight the financial effects of these diseases and the need for further research. We also found that the commercially available vaccine could neither prevent sub-clinical effects (lamb growth rate) nor clinical manifestations (pneumonic lung lesions) of lamb pneumonia. The case-control study has revealed farm-level factors which, in the absence of effective vaccines, indicated management practices that farmers might perceive as opportunities to control lamb pneumonia. However, it is advisable to evaluate the efficiency of such management changes. Pneumonia is aetiologically complex disease involving the interplay of many environmental, host and pathogen factors. It is also a difficult disease to study in the absence of diagnostic tests in live animals. However, further research should focus on the development of management changes until effective vaccines are available. A starting point for this research would be to evaluate the impact of such management changes in reducing the incidence of lamb pneumonia. More specifically, the roles of stress during crowding of lambs for extended periods warrants further investigation. The development of efficient vaccines requires an analysis of pathogens, especially Pasteurella (Mannheimia) haemolytica and Mycoplasma species, the sources of infection, their strain diversity and transmission dynamics.

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Kerslake, Joanne Isabel. "Improving triplet lamb survival in New Zealand : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palmerston North, New Zealand." 2010. http://hdl.handle.net/10179/1530.

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This thesis sets out to identify physical and physiological differences between lambs of different birth ranks at birth, and to use this information to identify practical on-farm management strategies which could improve triplet-born lamb survival. Triplet-born lambs, especially the lightest-triplet-born lambs, not only had a greater capacity to lose heat but also had a reduced capacity to produce heat when compared to twin-born lambs. Due to their lighter birth weights, triplet-born lambs had lower plasma thyroxine (T4) concentrations within the first 24 hours of life than twin-born lambs, and within twin- and triplet-born litters, the lightest- and medium-triplet-born lambs had greater plasma lactate concentrations than all twin-born lambs and the heaviest-triplet-born lambs. Independent of lamb birth weight, triplet-born lambs had lower plasma tri-iodothyronine (T3) concentrations within five minutes of birth, and within twin- and triplet-born litters, the lightest- and medium-triplet-born lambs had lower plasma T4 and T3 concentrations within five minutes of birth than all twin-born lambs and the heaviest-triplet-born lambs. It was hypothesised that because triplet-born lambs had a lighter birth weight and lower plasma thyroid hormone concentrations, they would have inadequate thermoregulatory capabilities when compared to twin-born lambs. The lower rectal temperatures of triplet-born lambs within the first hour of life and the lower heat production on a per lamb basis at 24 to 36 hours of age, and the lack of difference in maximum heat production on a per kg of birth weight basis at 24 to 36 hours of age support this hypothesis. Two practical on-farm management strategies trialled in this thesis to improve triplet-born lamb thermoregulation were offering concentrate supplement during late pregnancy to improve lamb birth weights, and maternal iodine supplementation to improve lamb plasma thyroid hormone concentrations. While offering concentrate showed positive effects such as increasing lamb birth weights, colostrum uptake and triplet-born lamb heat production on a per kg of birth weight basis, the results were either inconsistent across experiments or between birth ranks suggesting additional work is required to determine the repeatability and cost effectiveness of these findings. Maternal iodine supplementation offered no iv | P a g e benefits in terms of lamb birth weights, plasma thyroid hormone concentrations or lamb heat production. Further investigations identified that lamb birth weights, thyroid hormone concentrations, glucose and NEFA concentrations are positively associated with maximum heat production at 24 to 36 hours of age. Practical on-farm management strategies which could target these physical and physiological factors may improve triplet-born lamb heat production, and therefore the survival rates of triplet-born lambs.

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Rossi, José Luis. "A study of the interactions between Holstein-Friesian genotypes and feeding systems, with emphasis on system performance and cow grazing ability : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science, Institute of Veterinary, Animal and Biomedical Sciences, Massey University, New Zealand." 2006. http://hdl.handle.net/10179/1457.

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Imported genetic material of the Holstein-Friesian breed from overseas (OS), mainly from North America, has been used in New Zealand (NZ) since the late 1960's. This has diluted the genetic base of the former NZ Friesian genotype selected under intensive seasonal pasture-based systems. As a result, increased concerns have been raised about the negative influence of these overseas genes on the modern NZ Holstein- Friesian, as it is apparent that the OS Holstein-Friesian has a lower capacity to perform on grazed pasture. The objective of the present thesis was to investigate differences in production performance between three Holstein-Friesian genotypes farmed at different feed allowances (FA) on pasture-based systems; in addition, to investigate differences in the grazing process between strains under contrasting managements and sward conditions, and so to identify animal and pasture factors that affect the herbage intake (DMIH) and performance of the grazing cow. An accurate procedure was also established to estimate DMIH for cows fed forage and maize supplements, grazing in groups. Two modern high breeding worth (BW) Holstein-Friesian strains from NZ (NZ90) or overseas (OS90) origin and a low BW 1970's NZ Friesian genotype (NZ70) were farmed in two field experiments: (1) a long-term 'system' study that compared the yield performance of these genotypes in a range of systems with different feed allowance (FA) per cow, and a (2) a short-term 'component' study that compared the grazing capacity of the strains under contrasting sward conditions but at a common daily herbage allowance. The differences in productive performance between genotypes increased as the study progressed in the system study, with the largest observed in the last season. The mean milksolids (MS) yield per cow and per hectare were higher in NZ90 (395 kg cow-1 and 1,236 k ha-1) than in the NZ70 (336 kg cow-1 and 1,093 kg ha-1) and the OS90 (377 kg cow-1 and 1,154 kg ha-1). The higher production of NZ90 cows was supported by their higher mean daily MS yield than the NZ70 (1.45 vs. 1.21 kg MS cow-1 day-1) and more days in milk than OS90 cows (271 vs. 257 DIM). The lower lactation length of the OS90 strain occurred due to its lower body condition score (BCS) in late lactation, which determined an early dry-off for these cows. The lowest BCS of OS90 at the nadir (irrespective of FA), during lactation and at dry-off indicate these cows mobilised greater amount of body reserves and partitioned most of the energy ingested to yield. Genotype by FA interactions for milk and lactose yields, protein content in the milk and BCS were observed in the second and third seasons of the 'system' study. Milk yield increased as FA increased to a greater extent in OS90 than in the two NZ strains, whereas the content of solids in milk, particularly protein, increased to a greater extent for NZ90 than in both OS90 and NZ70. During lactation DMIH was higher for NZ90, intermediate in OS90 and lower in NZ70 (14.5, 13.9 and 12.6 kg DM cow-1 day-1 respectively for NZ90, OS90 and NZ70, as measured with nalkanes), and declined as lactation progressed, with a smaller difference for the total intake achieved (15.5, 15.2 and 13.1 kg DM cow-1 day-1 respectively) due to the increased supplement consumption. These results indicate that the OS90 needs more feed with a higher proportion of supplement in the diet to improve productive performance on pasture-based systems; the NZ90 would perform better when cow nutrition is mainly supported by grazing pasture, although further increments in performance could be expected from strategic supplementation, but requiring more feed than NZ70. The DMIH per unit of live weight (DMIH/LW) was highest in NZ90 strain in both the 'system' and in the short sward of the 'component' study (31.5 and 31.1 g DM kg-1 DM in NZ90 vs. 28.9 and 28.6 g DM kg-1 DM for OS90 in 'system' and 'component' studies respectively). The higher intake of NZ90 on pasture was sustained by a higher capacity to graze short swards than NZ70 and OS90, and to deal with the herbage of higher bulk density and lower quality present at the base of taller swards. The NZ90 can maintain DMIH in swards with different structures, indicating higher flexibility to perform under different managements and sward conditions. The size of the jaw is smaller in NZ90 than OS90 (88.4 vs. 92.4 mm) with effects on bite area and bite size, and this flexibility to adapt the size of the bite to swards of different structure may improve bite penetration under constraining sward conditions. The reduced ability of the OS90 to adjust ingestive behaviour to different swards would limit the capacity of this strain to perform on pasture. The fact that OS90 cows increased DMIH and DMIH/LW substantially in a leafy and taller sward (up to 21.6 kg cow-1 and 40.8 g DM kg-1Lw vs. 19.2 kg cow-1 and 41.0 g DM kg-1Lw in NZ90 during early lactation) suggests that yield performance can be improved in these cows even on pasture, by fine-tuning pasture management.

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Ramírez-Restrepo, Carlos Alberto. "Nutritional studies on Lotus corniculatus containing condensed tannins to increase reproductive rate and lamb growth under commercial dryland farming conditions : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science at Massey University, Palmerston North, New Zealand." 2004. http://hdl.handle.net/10179/1734.

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Five rotational grazing experiments were carried out at Massey University's Riverside farm, in the Wairarapa, on the East Coast of the Southern North Island, New Zealand, to compare the effects of feeding Lotus corniculatus L. (birdsfoot trefoil; cv. Grasslands Goldie) or perennial ryegrass (Lolium perenne)/white clover (Trifolium repens) dominant pasture upon sheep year round productivity. These studies also investigated under grazing, seasonal and annual net herbage accumulation rate and seasonal dynamics of undisturbed (i.e. non-grazed) net herbage accumulation rate of L. corniculatus relative to that of grass-dominant pasture. Aspects of in vivo digestibility of dry matter (DMD), organic matter (OMD), digestible organic matter in the dry matter (DOMD) and metabolisable energy (ME) concentration of L. corniculatus at different stages of maturity over the spring, summer and autumn were investigated in three indoor digestion trials. 1. Two field experiments (Chapter 2) were conducted during spring to assess the effects of grazing mixed age undrenched ewes on L. corniculatus (n = 50) or pasture (n = 50) and their lambs (mainly twins) on live weight (LW), wool production, faecal nematode egg count (FEC) and dag score. In Experiment 1 (18 October 2000 to 21 January 2001) and Experiment 2 (3 October 2001 to 2 January 2002) both forages were fed ad libitum. Total condensed tannin (CT) concentration in the diet selected was 24 to 27 g CT/kg DM for L. corniculatus and 1.4 to 1.5 g CT/kg DM for pasture. The LW gain, weaning LW and wool production were consistently greater (P < 0.001) for lambs grazing L. corniculatus, in either Experiment 1 (258 vs. 189 g/day; 36.1 vs. 30.1kg; 1.17 vs. 0.98 kg) and in Experiment 2 (247 vs. 162 g/day; 31.8 vs. 24.1kg; 1.17 vs. 0.81 kg), respectively. Ewe and lamb dag scores were strongly and positively correlated with dag weight (P < 0.001) and generally increased with time in sheep grazing pasture, whilst grazing on L. corniculatus consistently reduced dag score. FEC in ewes grazing pasture showed a post-parturient rise (PPR) following lambing, whilst ewes grazing L. corniculatus had a reduced PPR in FEC. Up to day 70, FEC in lambs grazing L. corniculatus was lower than that for lambs grazing pasture, but between day 70 and the end of both experiments (approximately day 90), FEC in lambs grazing L. corniculatus increased to similar values as for pasture-fed lambs. FEC was not correlated with dag score or dag weight in ewes or lambs grazing pasture, but these indices were weakly and positively correlated in ewes and lambs grazing L. corniculatus, suggesting that lowering FEC on L. corniculatus also reduced dag formation. It was concluded that under dryland farming conditions, the use of L. corniculatus during the spring/early summer lactation period can increase lamb LW and wool production, whilst eliminating the need for pre-lambing anthelmintic drenching and probably reducing the amount of insecticide needed to control flystrike. These effects compared to pasture are probably due to higher digestibilty, higher ME concentration, higher voluntary feed intake (VFI), and to the effect of CT in reducing rumen protein degradability and controlling internal parasites in sheep grazing L. corniculatus. The absence of endophyte in L. corniculatus may have also have contributed to these effects. 2. During 2001 and 2002 (Chapter 3), grazing trials from February to November were conducted for 279 days (Experiments 1) and 285 days (Experiment 2), to compare the effects of grazing shorn mixed age Romney ewes in light condition on L. corniculatus versus pasture during the mating period (9 weeks, Experiment 1) and 11 weeks (Experiment 2). In Experiment 2, the length of time (days) that ewes need to graze L. corniculatus before mating to maximise reproductive performance was also investigated. Common objectives in both Experiments were to measure forage feeding effects on ewe wool production and LW of their lambs at weaning. In Experiment 1, groups of ewes (n = 100) were fed on either L. corniculatus or pasture at a herbage allowance of 1.8 kg green DM/ewe/day for the first three weeks of feeding and increased to ad libitum (2.3 kg green DM/ewe/day) during the mating period for two cycles. In Experiment 2, groups of 75 ewes grazed L. corniculatus for 42, 21, 10 and 0 days before a synchronised oestrus, with pasture being grazed for the balance of the 42 days. All L. corniculatus groups continued grazing L. corniculatus for a further 5 weeks. Feed allowance was initially 2.0 kg green DM/ewe/day, increased to 2.3 kg green DM/ewe/day during the mating period over the two cycles. At the end of L. corniculatus feeding in both experiments the groups were combined and grazed on pasture until weaning. Total CT concentration in the diet selected was 18 to 29 g CT/kg DM for L. corniculatus, with only trace amounts in pasture. In Experiment 1 mating ewes on L. corniculatus compared to pasture increased number of lambs born and lambs weaned per ewe lambing by 16 and 32% units respectively (P < 0.05), due to more multiple and less single births (P = 0.06) and to reduced lamb mortality (P < 0.05) between birth and weaning. In Experiment 2, increasing the numbers of days of grazing L. corniculatus before ovulation (0, 10, 21, 42 days) linearly increased ovulation rate (P < 0.05), lambs born and lamb weaned by up to 16% units, but had no effect upon lamb mortality. Mating ewes on L. corniculatus increased wool production (P < 0.01) and fibre length (P < 0.05) in Experiment 1 but not in Experiment 2. Grazing L. corniculatus had no effect on lamb birth weight and only small positive effects on weaning LW. It was concluded that, under commercial dryland farming conditions, the use of L. corniculatus during the mating season in late summer/autumn can be used to increase reproductive efficiency and wool production, with the largest responses in years with exceptionally dry autumn periods. These effects are probably due to the higher digestibility and ME concentration of L. corniculatus than pasture and to the CT in L. corniculatus reducing rumen protein degradability and leading to greater essential amino acid (EAA) absorption from the small intestine. Effects of forage CT upon the uterine microenvironment at the time of conception, implantation and early foetal growth, need to be investigated in future studies. It is also suggested that effects of mating on L. corniculatus upon lamb mortality between birth and weaning should be further investigated with ewe numbers/treatment increased from 100 to 350. 3. During the summer of 2002/2003, another grazing trial (Chapter 4: 95 days) compared the effects of grazing L. corniculatus and pasture on LW and the dynamics of nematode parasite infection in Suffolk x Romney weaned lambs fed ad libitum. Half of the lambs (n = 30) grazing either L. corniculatus or pasture received oral anthelmintic at the start and at monthly intervals (regular-drenched groups), whilst the remaining 30 lambs in each treatment only received oral anthelmintic when mean faecal nematode egg counts (FECs) exceed 1,000 eggs/g wet faeces (trigger-drenched groups), which occurred on day 58 only for both groups. Trigger and regular-drench lambs grazed separate areas. Total CT concentration in the diet selected was 40 to 31 g CT/kg DM for L. corniculatus, with only trace amounts in pasture. Regular-drenched lambs grazing L. corniculatus had significantly higher LW gain (298 g/day) and carcass weight gain (133 g/day) than all the other groups, whilst trigger-drenched lambs grazing L. corniculatus had significantly greater LW gain (228 g/day) and carcass gain (99 g/day) than regular-drenched (200; 66 g/day) and trigger-drenched (187; 63 g/day) lambs grazing pasture. Carcass fatness was significantly lower for trigger-drenched lambs than for regular-drenched lambs, when fed either L. corniculatus or pasture. Dag score was consistently lower for regular-drenched lambs grazing L. corniculatus than pasture; trigger-drenched lambs showed similar effects up to day 48, with no differences between the two groups thereafter. Regular anthelmintic treatment maintained FECs at low values, while parasitised lambs on L. corniculatus tended to have higher FECs than pasture-fed lambs. Relative to trigger-drenched lambs that grazed pasture, grazing trigger-drenched lambs on L. corniculatus had significantly reduced worm burdens of Haemochus contortus, Teladosargia spp., Nematodirus spp. and Cooperia spp. at slaughter, but greater burdens of Trichostrongylus spp., Chabertia ovina, Oesophagostonum spp. and Trichuris ovis were present in L. corniculatus-fed lambs. It was concluded that grazing L. corniculatus under dryland farming conditions compared to pasture can increase LW gain of weaned lambs, whilst reducing reliance on anthelmintic drenches to control parasites. These effects are probably due to increased protein supply from the action of CT enabling the lambs to have a higher LW gain when carrying a parasite burden, and to L. corniculatus better maintaining its high ME value under drought conditions. Using L. corniculatus to finish weaned lambs without anthelmintic drenches for a seven-week period is proposed. 4. A three-year study (Chapter 5; November 2000 to October 2003) was conducted to compare, under grazing conditions, seasonal and annual grazed net herbage accumulation rate and seasonal dynamics of undisturbed (i.e. non-grazed) net herbage accumulation rate of L. corniculatus relative to grass-dominant pasture. Prediction equations to estimate standing DM in L. corniculatus and pasture from the rising plate meter (RPM) and sward surface height were also generated. L. corniculatus and pasture growing in a moderate fertility and low-pH soil (pH 5.35) accumulated similar total herbage masses (24.3 vs. 24.1 t DM/ha) over the 3-year period, with the DM production being greater for L. corniculatus than for pasture during 2000-2001, producing more DM during summer/autumn drought conditions. The net herbage accumulation rate from undisturbed areas of L. corniculatus and pasture were similar in spring, summer and autumn. Seasonal variation in the calibration regressions fitted to estimate herbage mass of L. corniculatus non-destructively, suggested a combination of destructive and nondestructive methods are needed to assess herbage mass. It was concluded that L. corniculatus has the potential to increase the performance of a pasture-based sheep dryland farming system due to its ability to grow in acidic soils, its tolerance of drought conditions during summer/autumn and its seasonality of feed supply. 5. Three digestion experiments involving cryptorchid weaned lambs were conducted for 14 days over the spring, summer and autumn to determine changes in in vivo digestibility of DM, OM, digestible OM in the DM and ME concentration of L. corniculatus at different stages of maturity. In vivo digestibility samples were then used as standards to investigate if the enzymatic in vitro system of Roughan and Holland (1977) could predict OMD and DOMD of CT-containing L. corniculatus. Digestibility of L. corniculatus declined as it matured, but the rate of decline was much less than occurs for temperate grasses and for white clover. It was concluded that the in vitro enzymatic system of Roughan and Holland (1977) can be used to predict OMD and DOMD of L. corniculatus, provided a standard curve involving in vivo data generated with L. corniculatus is used. Using a standard curve with in vivo data from pasture led to bias which increased at lower OMD values. Reasons for the consistent differences between L. corniculatus and pasture standard curves are discussed, including possible effects of residual bound CT in lowering in vitro digestibility. From this series of experiments, this study is the first to report that relative to conventional perennial ryegrass/white clover, mating ewes on L. corniculatus under grazing conditions may reduce post-natal lamb mortality. It is also the first study to show that grazing sheep on L. corniculatus can maintain productivity during spring and summer with reduced dependence on anthelmintic drench input. It is concluded that whole farm modelling, mechanical harvesting and conservation strategies, selection of L. corniculatus germplasm for creeping-type plants more suited to grazing and the integration of new crops containing secondary compounds, such as chicory, should be considered to support major advances in sustainable dryland sheep farming systems.

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Chaves, Alexandre Vieira. "Digestion characteristics of forages, including perennial ryegrass at different stages of maturity, and supplementary feeding for dairy cows grazing pasture : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Animal Science, Institute of Food Nutrition and Human Health, Massey University, Palmerston North, New Zealand." 2003. http://hdl.handle.net/10179/1160.

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This thesis defines digestion kinetics for perennial ryegrass (Lolium perenne L.), which is the main component of diets fed to dairy cows in New Zealand. Chemical composition and digestion kinetics were measured in fresh minced ryegrass as it matured and leaf, stem and inflorescence of several grass species. In sacco and in vitro incubations were used to define rates of degradation and nutrient release. Two short-term grazing trials were used to evaluate contrasting silages as supplements for cows fed restricted amounts of summer pasture. The minced preparation of ryegrass resulted in a similar distribution of dry matter (DM) between particle size fraction and rumen digesta from cows fed pasture. Mincing released 0.46 – 0.80 of crude protein into the soluble fraction, with highest proportions for mature grasses which had low CP concentrations (about 8 g CP/100 g DM). In contrast, the majority of fibre remained in the insoluble fraction but rates of degradation (k) approximately halved as grass matured. In vitro yield of VFA was similar for immature and mature minced ryegrass (after 12 hours VFA was equivalent to about 30% of DM), even though ammonia concentration declined to very low values for stem and mature grass. This suggests the rapid initial microbial growth was able to sustain a high level of DM degradation to VFA with mature grass. The summer pasture used for silage supplementation was of uncharacteristically good quality so the expected contrasts between maize, pasture, sulla (Hedysarum coronarium), lotus (Lotus corniculatus) and sulla/maize silage mixtures were less than expected. Milk responses to lotus silage supplements were greater than other silages (e.g.: 290 g milksolids from 54 MJ ME by lotus versus 110 g milksolids from about 50 MJ ME supplied by other silages). Pasture substitution was low (0.06 – 0.33). The Cornell Net Carbohydrate and Protein System (CNCPS) was chosen for evaluation of cow trial data because it uses feed degradation parameters as input variables to estimate nutrient supply. Model prediction of milk yield matched observed values when cows maintained liveweight. Milk yield was underestimated at low intakes and overestimated at high intakes because no allowance is made for nutrient partitioning between milk production and liveweight change.

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Dissertations / Theses on the topic 'Fields of Research – 300000 Agricultural, Veterinary and Environmental Sciences – 300400 Animal Production'

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