Academic literature on the topic 'Fields of Research – 270000 Biological Sciences – 270500 Zoology'

The species status and host range of Heliothis armigera was investigated. DNA profiling, mitochondrial DNA sequencing, RAPD's, along with traditional morphological and crossing methods were used to investigate micro, macro and mega-population structuring. Thirty-six new host records were added, of which a number are important common weeds and crops. Mortality due to parasitoids and fungal infections were quantified on many host species. Genetic analysis of the COII and the AT-rich regions of the mitochondrial DNA showed very high levels of variation, as did the DNA profiling using the probes (CA)n and 33.15. The morphological analyses also showed variation, but to a lesser degree and without statistical significance. The variation found at all levels and in all aspects is discussed with respect to caterpillar host-plants and geographical location. All host-plant populations of caterpillars showed very high levels of genetic variability. However, the population of caterpillars found on Sulla (Hedysarium coronarium) was significantly more variable than those found on Lotus and Lucerne when compared using DNA profiling. The sequences obtained from the two mtDNA regions also showed considerable variation, a great percentage of which was uninformative. This variation did not allude to any structuring of caterpillar populations with respect to host-plant or geographical location. H. armigera is genetically a very variable species which does not equate with any population structuring present in the host-plant or geographical populations investigated here. This study has greatly increased the general understanding of this insect, and has elucidated a portion of the genetic makeup, but not helped in the development any new control methods.

Although Argentine ant(Linepithema humile), a highly invasive ant species, has been in New Zealand for at least l4 years, little is known about their ecology and potential for invasion. Increasing spread and establishment of populations throughout New Zealand is disturbing because of the devastating impacts documented on native invertebrate biodiversity overseas. The primary aim of this study was to determine the impacts of Argentine ants on arthropods in native forest habitats in west Auckland. Pitfall traps at invaded and uninvaded sites were used to quantify ant and non-ant arthropod faunas. Argentine ants did not adversely affect native host ant communities. Moreover, two ant species appeared to be resistant to invasion. Argentine ant invasion reduced the abundance of a few orders of invertebrates while several taxa were more abundant in the presence of Argentine ants. Distribution and foraging activity of Argentine ant populations were monitored in this study from 2000-2003. Also, rate of spread was investigated to evaluate whether native forest habitats would be at risk from invasion. Measurements of foraging ant trails on monitored tree trunks revealed seasonal distribution patterns involving high activity in summer/autumn and low activity in winter/early spring. Argentine ants were found to be established primarily along the edge of the forest and did not invade into the interior of the forest during the study period. An Argentine ant poisoning operation on Tiritiri Matangi Island in January 2001 provided the opportunity to document the results of the eradication trial. In addition, pitfall traps placed at two treated sites and one untreated site were used to compare pre-poison and post-poison effects on ant and non-ant invertebrate communities. Fipronil baiting at 0.01% effectively reduced Argentine ants at the study sites and very few ants were observed in both tree count and pitfall trap recordings two months after poisoning. The invasion of Argentine ants on Tiritiri Matangi Island decimated native host ants and no recovery was detected throughout the study. Several groups of invertebrates appeared to benefit from the removal of Argentine ants while a few showed no detectable changes. Conservation implications resulting from the findings of this study are discussed Also, potential future research involving Argentine ants are outlined.

The vegetable brassicas of cabbage, broccoli, and cauliflower are grown in Pukekohe for the Auckland fresh-produce markets. These brassicas are attacked by three major lepidopterous pests: diamondback moth (DBM) (Plutella xylostella (L)) (Yponomeutidae), white butterfly (WB) (Pieris rapae (L.)) (Pieridae), and soybean looper (SBL) (Thysanoplusia orichalcea (F.)) (Noctuidae). Current grower strategy to combat these pests is calendar-scheduled insecticide applications. The goal of this thesis is to develop pest management alternatives. The seasonality of these three pests is discussed. DBM and WB are each under biological control by a larval and a pupal parasitoid, but this natural control is not sufficient to allow economic harvests in cabbage and was not synchronized. No parasitoids of SBL were found. The importation of additional natural enemies is discussed. A scouting system of the percent of cabbage plants infested coupled with an action threshold of. 15%-20% infested plants, resulted in good yields in field trials and allowed up to a 50% reduction in insecticide applications over the growth period when compared to a 14-day calendar schedule. Implementation of the 15% infested threshold in commercial cabbage fields resulted in up to an 83% reduction in insecticide applications with no yield decrease in quality or quantity. Application of this 15% infested plant threshold to broccoli and cauliflower decreased insecticide applications by 40% and 17%, respectively. Study of larval biology indicated that all of the lepidopterans preferentially fed on leaves; timing of the first insecticide application in broccoli and cauliflower to coincide with floret initiation decreased insecticide applications by 80% and 67%, respectively. Laboratory and field trials comparing DBM oviposition preference, larval survivability, and parasitism rates between cabbage, broccoli, and cauliflower are discussed. Knowledge of lepidopterous pest seasonality and biology, linked to careful timing of insecticide applications to coincide with threshold levels of pests, can take full advantage of natural enemies and reduce insecticide input in the vegetable brassicas of cabbage, broccoli, and cauliflower with no decrease in crop quality.

The thesis answers the general question of whether the quality of artificially reared insect species should be based on performance tests for intended use or whether quality should be based on a more holistic biological approach. The empirical research is carried out using the lepidopteran leafroller 'Cnephasia' jactatana (Walker). The thesis defines biological performance and quality in terms of the success of an insect population in survival and reproduction and regards the laboratory environment as an artificial habitat that insects must colonise in order to survive and reproduce. Changes in biological performance that occurred during 12 successive generations of laboratory rearing were due to selection, acclimatisation and domestication and not adaptation. Artificial colonisation is theoretically successful within a limited range of environmental factors. As the inherent genetic variability of the founder population determines the resilience of the population to changes in performance, the ranges of environmental factors during colonisation should be wide to 'capture' much of the variability. Using body size (weight) as an aspect of overall quality, the thesis presents evidence that the final instar larva of C. jactatana has a threshold mechanism (larval critical weight, LCW) that determines pupal and adult size. There is a proportionate decrease in weight from the maximum weight that a larva attains in the final instar (LMW) to pupa ( described as constant DP ) and to adult (DA). There is a direct relation between the latent feeding period (period between attaining an LCW and LMW), LMW, pupal and adult size, and the reproductive performance (fecundity ). Within the experimental conditions diet quality, temperature, photoperiod and artificial selection had no effect on the larval critical weight, DP or DA, the larval threshold mechanism in C. jactatana is probably a mechanical trigger that initiates pupation. Diet quality, temperature and thermophotoperiods affected pupal size, adult size and reproductive performance. Photoperiod had no significant effects on size and reproductive performance. Positive assortative selections for slow development and low pupal weight significantly decreased pupal and adult size, and reproductive performance. Selection for fast development and heavy pupal weight for three generations had no significant effect on size or reproductive performance. Larval critical weight is demonstrated as useful to define quality indices and predict the performance of laboratory reared insects. The general conclusion of the thesis is that insect quality should be defined more in terms of the success in survival and colonising ability rather than solely on the success for 'intended role' or 'fitness for use'.

The first aim of this thesis is an introduction to some basic aspects of multivariate control theory which are relevant to the question of how the brain controls movements. A regulator is a device which forces a system to follow a specified trajectory in the presence of perturbations which might cause it to diverge from that trajectory. Regulation involves constructing an additional control input which depends upon the difference between the actual system state and the desired state. This requires the construction of a state estimate from raw data about system input and output. For effective state estimation, the sensor input gain to the state estimator needs to be time-varying. Under certain assumptions, the appropriate input gain can be specified analytically. The feedback regulation signal can then be constructed as a function of the state estimate. For effective regulation, the gain of the feedback function has to vary during maneuvers. Under certain assumptions an appropriate feedback gain can be specified analytically. The state observer input gain equations have a simple relationship to the feedback gain equations, so that gain specification is essentially the same task in each case. Cerebellar research has been dominated for the past 25 years by the theories of James Albus and David Marr. These mathematicians proposed similar models in which certain synapses in the cerebellar cortex are continuously modified by experience in such a way that movements which are consistently repeated under a given set of circumstances come to be performed automatically by the cerebellum. Much experimental work has focussed on the role of the vestibulo-cerebellum in fine control and learning of the vestibulo-ocular reflex. The state of the art along this line is formally described by Fujita's adaptive filter model of the cerebellar cortex. In chapter 4 it is shown that a basic feature of Fujita's model is inconsistent with available evidence. The 'Tensorial theory of brain function' is discussed in chapter 5. This is a novel theory of brain function which has been used in an attempt two explain cerebellar function. The attempt is a failure, based on sophistocated misconceptions and flawed by poor reasoning and clumsy analysis. The approach serves to confuse rather than clarify the question of cerebellar function. The final chapter of the first part of the thesis presents a basis for a new approach to cerebellar function based on the engineering theory of control of multivariate dynamical systems. It is proposed that the cerebellum is involved in movement regulation by controlling the gains of brainstem motor pathways, and in mapping the animal's environment by controlling the gains of sensory inputs to the midbrain. While learning undoubtedly does occur in the cerebellar cortex, this is not specifically a 'learning device', as commonly conceived. The second part of the thesis is concerned with the development and application of a method of system identification for characterising the dynamics of the vestibulo-ocular reflex and its components in an elasmobranch. The chosen method involves pulse-rate modulated bilateral electrical stimulation of the horizontal semicircular canal ampullary nerves. This produces a synthetic vestibulo-ocular reflex in a stationary preparation. The stimulus pattern is a pseudorandom binary sequence of pulse rates, so that cross-correlation of the stimulus pattern with the response signal gives a Unit Impulse Response dynamic signature for the system. Computer software for signal generation, recording, analysis and display was written by the author. The identification system was applied first to characterise the dynamics of the eye movement response to horizontal canal ampullary nerve stimulation, and compare this to the dynamics of the eye motor plant alone. The eye motor preparation acts as a first order low-pass filter with a time constant of about 0.2 seconds (16°C), while the ampullary preparation acts as a second order low-pass filter with a dominant time constant of about 0.75 seconds (16°C). Central pathways of the elasmobranch vestibulo-ocular reflex extend the time constant of the motor plant by a factor of 3-4, as in other animals. Eye movements predicted by fitted linear models accurately mimic eye movements recorded during experiments, suggesting both that central pathways of the reflex operate normally during this somewhat un-naturally evoked response and that the identification procedure is effective. Furthermore, combination of the ampullary nerve to eye movement transfer function obtained in this study, with head rotation to ampullary nerve transfer functions obtained by other workers, gives a consistent picture of elasmobranch vestibulo-ocular reflex function predicting compensatory eye movements in the band 0.2 - 4.0 Hz., and perhaps higher. The identification method has also been applied to produce models of vestibulocerebellar Purkinje cell dynamics during electrically evoked vestibular eye movements. Linear identification gives a poor characterisation of Purkinje cell activity during the high frequency vestibulo-ocular reflex. This is incompatible with linear phase-compensator models of the cerebellar cortex, but consistent with the reflex gain modulation theory of cerebellar function advocated in the first part of the thesis.

The skua (Family Stercorariidae) are a group of large, gull-like, predatory seabirds. Two skua species are found in the Antarctic region; the south polar skua (Catharacta maccormicki) and the brown skua (C. lonnbergi). The breeding distribution of the former, is restricted to the Antarctic continent and nearby islands, while the latter has a circumpolar distribution which extends northward from the Antarctic Peninsula and includes many of the Southern Ocean islands. The south polar skua is strictly monogamous, while in contrast, a number of populations of brown skua are comprised in part of communally breeding groups. The brown skua represents the only known example of a communally breeding seabird. In every skua species, breeding females are on average larger and heavier than males. However, in most skua species this dimorphism is relatively small and is of only limited use in sexing individuals. The discovery of sex-specific fragments in the DNA fingerprints of the south polar skua is reported. The multilocus probe pV47-2 hybridised to Hae III restriction fragments which were present exclusively in females and therefore presumably W-linked. The presence of these sex-specific fragments were used to identify female adults and chicks. In addition, the use of these fragments as potentially informative maternal markers is discussed. The parentage of the 13 families from two populations from Ross Island, Antarctica, determined by DNA fingerprinting, revealed a single instance of extra-pair paternity and a single instance of a chick which was parented by neither resident adult. The most likely explanation for the latter is the 'adoption' of a chick from a neighbouring territory. Similarly, DNA fingerprinting was used to assign the sex of individuals of brown skua from a population which breeds on the Chatham Islands, New Zealand. A large proportion of the Chatham Islands population breed in communal groups. Each communal group was shown to be comprised of a single female and two or more males. Consequently, the overall sex ratio amongst breeding birds was biased, with almost twice the number of males as females. In contrast the sex ratio amongst fledgling chicks did not differ significantly from 1:1. The patterns of reproductive success in breeding pairs and communal groups of the brown skua from the Chatham Islands population were determined using multilocus DNA fingerprinting. Sixteen breeding groups were examined, the parentage of 45 chicks produced over three breeding seasons was established using the probes 33.15 and 33.6. No evidence was found of either extra-pair paternity or extra-group fertilisation and there was no evidence of egg dumping by females in any breeding group. These results suggest that long-term banding records for breeding pairs and communal groups accurately reflect the overall reproductive success of these individual groups. In addition, preliminary band sharing analysis indicated that adult members of communal groups were not closely related. These findings are also supported by banding records and are in contrast to the findings of the majority of communally breeding species studied. In the 10 communally breeding groups examined, multiple paternity within a clutch was recorded on two of the 12 occasions in which two chicks were reared. Furthermore, analysis of parentage of the chicks belonging to communal groups in which the adults had remained unchanged for two or more seasons showed that some males had variable reproductive success in different seasons. These records suggest that estimates of reproductive success of individuals based on a single season's data can be misleading. Should temporal changes in paternity (and/or maternity) be shown to be common phenomena in other species, this would have major implications for the interpretation of many parentage studies. The explanation of altruistic behaviour is one of the central issues in contemporary evolutionary theory and behavioural ecology. One of the best known examples of apparent altruism is the helping behaviour which occurs in communal breeding groups such as those found in the brown skua. Within these groups individuals often help to raise offspring which are not their own. This behaviour is an apparent enigma in a world in which organisms are assumed to act in a selfish manner. Consequently, this behaviour has become a focal example at the centre of much evolutionary debate. A variety of theories have been suggested to explain helping behaviour, the most recent is that helping is an unselected consequence of the evolution of communal breeding. This hypothesis is discussed in relation to the recent literature and it is concluded that it does little to advance the current debate. An alternative theoretical approach to helping behaviour is outlined. In conclusion the general findings from the investigation of communal breeding in the brown skua are summarised and these findings are discussed. Finally, possible areas of future research are outlined.
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The Pinnotheridae is a family of decapod crustaceans comprising more than 120 mostly microphagous and commensal species. As symbionts of a variety of aquatic invertebrates, pinnotherids typically live in an intimate association with their host depending on it for an almost lifelong source of nourishment and shelter, together with a site for mating. The New Zealand pinnotherid fauna was thought to comprise only one species, Pinnotheres novaezelandiae Filhol, associated with a multitude of hosts. Recently, however, a separate species, P. atrinicola Page, has been described which is regarded as being host specific to the horse mussel Atrina zelandica Gray. In this context, the relationship between pea crabs and their hosts is of special interest, and is the focus of this thesis. An investigation into the population dynamics of the symbiotic relationship between P novaezelandiae and its host, the green-lip mussel Perna canaliculus, at Westmere Reef, Auckland between May 1986 and July 1988 is reported. Ovigerous females and Stage I males and females were found throughout the sampling period, indicating that reproduction is continuous in this species. The developmental composition of the pea crab population reveals that soft-shelled males, usually regarded as an anomalous instar, formed a significant component of the pea crab population at all times. It is suggested that these individuals represent a distinct facies, analogous to the Stage II female instar. The presence of a pea crab was found to have a highly significant detrimental effect on mussel condition. Analysis of the distribution of pea crabs among the mussel population indicates mature crabs display a repulsed distribution favouring to live a solitary existence, whereas younger (pre-hard and Stage I) crabs showed a random distribution in broad agreement with a theoretical Poisson distribution. The biological status of the two described taxa was investigated by a survey of electrophoretically detectable genetic variation of populations from throughout the North Island of New Zealand. Pea crabs from 18 host populations from nine geographically disparate localities were subjected to cellulose acetate and poly-acrylamide electrophoresis. Forty-one enzyme systems were screened for polymorphism. Clearly resolved enzyme phenotypes were obtained at 23 presumptive loci, of which l5 exhibited polymorphism. An analysis of electromorph frequency data revealed that both taxa are highly genetically structured and typified by high levels of polymorphism and heterozygosity; results atypical of brachyuran crabs. P- atrinicola was found to exhibit strong patterns of geographic differentiation and clinal variation in electromorph frequency. Of particular significance is the pattern of genetic differentiation observed among populations of p. novaezelandiae. Hierarchical F-statistics indicated that the preponderance of inter-population differentiation can be attributed to differences in electromorph frequency among host-associated populations of P. novaezelandiae within a sampling locality. Geographic differentiation was a comparatively insignificant factor in the structuring of the sampled P. novaezelandiae populations. Individuals belonging to two genetically very distinct units were found within a newly recorded host species, Mactra ovata ovata Gray at Green and Wood Bays, Manukau Harbour. Hardy-Weinberg analyses indicate the host-associated populations of P. novaezelandiae exhibit such a pronounced pattern of homozygote excess and disturbance from genetic equilibrium in sympatry that it is unreasonable to consider them as a single panmictic population. It is concluded that significant biological discontinuities based on host origin exist within the currently recognised taxon. Such a conclusion is supported by data presented on qualitative differences in host recognition observed between different host-associated populations of P. novaezelandiae. Conservatively these discontinuities indicate host race development, although a viable alternate hypothesis would be the presence of cryptic, host-specific biological species within P. novaezelandiae. Hostrace development as found here is a well recognised phenomenon in insect-host and parasitoid-host relationships, although little studied in marine symbiotic relationships. Such a phenomenon has important implications for ecological, behavioural and physiological studies on marine symbionts in general.

The population dynamics of juvenile snapper, Pagrus auratus, were investigated in the Hauraki Gulf, north-eastern New Zealand, between 1982 and 1990. Attention focused on age and growth, temporal and spatial variation in abundance, and recruitment. Daily increment formation was validated in the sagittae of snapper up to about 160 days old. Increment width varied with time of year, and snapper age, and increments were not resolvable with a light microscope during winter. Increment counts inside a prominent metamorphic mark showed that larval duration was 18-32 days, and was inversely related to water temperature. Spawning dates were back-calculated from increment counts in settled juveniles, and ranged from September to March with a peak in November-January. The onset of spawning was temperature dependent. Fast-growing snapper had smaller sagittae than slow-growing snapper, indicating an uncoupling of otolith and somatic growth. Snapper gonads differentiated first as ovaries during the second year of life, and then some juveniles changed sex to become males during their third year. Sex change occurred before maturity, so snapper are functionally gonochoristic. Growth was slow during the larval phase, but increased rapidly after metamorphosis to about 0.6-0.9 mm.day-1. From the first winter, growth followed a well-defined annual cycle, with little or no growth during winter, and linear growth of 0.16-0.43 mm.day-1 during spring-autumn for 0+/1+ and 1+/2+ snapper. Snapper grew faster at higher temperatures. Trawl catch rates were affected by numerous gear and environmental factors, but probably provided reasonable estimates of snapper relative abundance. Recommendations are made for improving snapper trawl survey procedures. There was a strong annual abundance cycle in the Kawau region, peaking in spring, and declining to a minimum in winter. Snapper were patchily distributed at a spatial scale of 1-2 km, probably because of preference for specific micro-habitats. Year class strength of 1+ snapper varied 17-fold over seven years, and was strongly positively correlated with autumn sea surface temperature during the 0+ year. The strengths of the 1991 and 1992 year classes are predicted to be below average, and extremely weak, respectively.

This thesis develops a quantitative cladistic approach to panbiogeography. Algorithms for constructing and comparing area cladograms are developed and implemented in a computer program. Examples of the use of this software are described. The principle results of this thesis are: (1) The description of algorithms for implementing Nelson and Platnick's (1981) methods for constructing area cladograms. These algorithms have been incorporated into a computer program. (2) Zandee and Roos' (1987) methods based on "component-compatibility" are shown to be flawed. (3) Recent criticisms of Nelson and Platnick's methods by E. O. Wiley are rebutted. (4) A quantitative reanalysis of Hafner and Nadler's (1988) allozyme data for gophers and their parasitic lice illustrates the utility of information on timing of speciation events in interpreting apparent incongruence between host and parasite cladograms. In addition the thesis contains a survey of some current themes in biogeography, a reply to criticisms of my earlier work on track analysis, and an application of bootstrap and consensus methods to place confidence limits on estimates of cladograms.

Growth, development and behaviour were examined in artificially reared larval Pagrus auratus and Forsterygion varium, from the time of hatching. Yolk-sac larval P.auratus hatched at a small size (2.00mm SL), without functional eyes, mouth or digestive tract, and for three days spent long periods at rest. Growth was initially rapid but slowed by 3 days as yolk reserves neared depletion. By days 4-5, the mouth had opened, eyes were functional, yolk was depleted, and a rudimentary gut had formed. Larvae were now able to maintain a horizontal swimming mode and were actively searching for and attacking prey. First feeding was observed in some larvae. Growth was retarded during the transition from endogenous to exogenous nutrition and then increased as feeding proficiency improved. Yolk-sac F.varium hatched at a larger size (4.78mm SL), with functional eyes and jaws. Larvae were able to maintain a horizontal swimming mode from hatching. First feeding was observed from the first day after hatching. F.varium larvae grew steadily from the time of hatching. Ocular morphology was examined in larval, juvenile and adult P.auratus and F.varium. There was a 96 fold increase in eye size, from 0.23mm diameter in a 4 day old larval P.auratus (3.4mm SL) to a maximum diameter of 22mm in an adult of 333mm body length. F.varium displayed a 26 fold increase in eye size, from 0.28mm diameter in the smallest larva (5.00mm SL) to a maximum eye diameter of 7.2mm in a 11gmm long adult. Larval fish had pure cone retinae, however putative rod precursor cells were present from hatching in F.varium and from 18 days in P.auratus. Juvenile and adult fish had duplex retinae with cones arranged in a square mosaic in which 4 twin cones surround a central single cone. Hypertrophy of cone ellipsoids with increasing eye size, resulted in maintenance of a closely packed array in fishes of all sizes. The appearance of retinomotor movements was coincident with the development of a duplex retina in both species. Theoretical spatial acuity (calculated as a function of cone spacing and focal length of the lens) was poor in the smallest larval fish (2° 1' and 1° 8' minimum separable angle in 4 and 1 day old P.auratus and F.varium respectively) but improved to asymptotic values in adults (3'- 4', and 9' in P.auratus and F.varium respectively). Behavioural acuity (determined using the optokinetic response) of 4 day old larval P.auratus (37° 30') and 1 day old F.varium (29°) was very much lower than histological estimates. Behavioural acuity improved to 8° 8' in 16 day old P.auratus and 4° 18' in 14 day old F.varium, but did not attain theoretical estimates for fish of that size (55' and 54'). A rudimentary retractor lentis muscle was first apparent in larval fish 1 week after hatching, and was coincident with the formation of a posterior lental space. Presumably larval fish eyes were incapable of accomodative lens movements until this time. A relative measure of Matthiessen's ratio (distance from lens centre to boundary of the pigmented retinal epithelium/lens radius) measured histologically, decreased from 4.2 and 2.7 in 3 day old P.auratus and newly hatched F.varium, to 2.2 and 2.3 in larvae 22 and 16 days of age respectively. This suggests that growth of the retina and lens were not symmetrical in the eyes of very small larval fish. If Matthiessen's ratio holds for little eyes, then they will initially be strongly myopic. This may account in part for the mismatch between behavioural and theoretical acuity. Perceptive distances of first feeding larval P.auratus and F.varium, estimated for prey items equal in dimensions to maximum jaw widths, were very small (0.2mm and 0.4mm for prey 0.15mm and 0.2mm in size respectively), but increased with increasing body size to 2.1mm and 4.0mm for prey 0.3mm in size, at 16 and 14 days of age respectively. These data have implications for larval feeding in the wild.