Academic literature on the topic 'Field bioreactor'
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Journal articles on the topic "Field bioreactor":
1
Feyereisen, Gary W., Ehsan Ghane, Todd W. Schumacher, Brent J. Dalzell, and M. R. Williams. "Can Woodchip Bioreactors Be Used at a Catchment Scale? Nitrate Performance and Sediment Considerations." Journal of the ASABE 66, no. 2 (2023): 367–79. http://dx.doi.org/10.13031/ja.15496.
Abstract:
Highlights Novel three-bed, cascading-inlet bioreactor treated agricultural drainage from a 249-ha catchment. Nitrate removal rates and load reduction efficiencies were similar to those of traditional single-field bioreactors. Sedimentation problems reduced bed life; a sediment sensing and exclusion system solved them. This scale provides opportunities for centralized management and nutrient reduction verification. Abstract. Denitrifying bioreactors, a structural practice deployed at the field scale to meet water quality goals, have been underutilized and require additional evaluation at the small catchment scale. The objective of this study was to quantify the performance of a large, multi-bed denitrifying bioreactor system sized to treat agricultural drainage runoff (combined drainage discharge and surface runoff) from a 249-ha catchment. Three woodchip bioreactor beds, 7.6 m wide by 41 m long by 1.5 m deep, with cascading inlets, were constructed in 2016 in southern Minnesota, U.S. The beds received runoff for one water year from a catchment area that is 91% tile-drained row crops, primarily maize and soybeans. Initial woodchip quality differed among the three beds, affecting flow and nitrate removal rates. Bioreactor flow was unimpeded by sediment for twelve events from September 2016 to July 2017, during which time 55% of the discharge from the catchment was treated in the bioreactor beds. Average daily nitrate removal rates ranged from 2.5 to 6.5 g-N m-3 d-1 for the three bioreactor beds, with nitrate-N load removal of flow through the beds between 19% and 27%. When accounting for untreated by-pass flow, the overall nitrate-N removal of the multi-bed system was 12.5% (713 kg N). During high-flow events, incoming sediment clogged the reactor beds, decreasing their performance. There was 4,520 kg of sediment trapped in one bed, and evidence suggests the other two trapped a similar load. To solve this problem and prolong the bioreactor’s lifespan, we installed a shutoff gate that activated when inflow turbidity exceeded a threshold value. Finally, the findings indicate that catchment-scale denitrifying bioreactors can successfully remove nitrate load from agricultural runoff, but sediment-prevention measures may be required to extend the bioreactor's lifespan. Keywords: Bioreactor, Denitrification, Nitrate removal, Sedimentation, Subsurface drainage.
2
Hartfiel, Lindsey M., Michelle L. Soupir, and Kurt A. Rosentrater. "Techno-Economic Analysis of Constant-Flow Woodchip Bioreactors." Transactions of the ASABE 64, no. 5 (2021): 1545–54. http://dx.doi.org/10.13031/trans.14300.
Abstract:
HighlightsTechno-economic analysis was performed for multiple scales of bioreactors operated under a variety of conditions.The unit cost decreased as the bioreactor size increased.The unit cost increased in bioreactors with longer HRTs and bypass flow due to reduced treatment capacity.One large bioreactor was more cost-effective than multiple smaller bioreactors.Abstract. Woodchip denitrification bioreactors are a relatively new, edge-of-field technology used to reduce nitrate-nitrogen (NO3-N) from subsurface tile drainage. The removal rate of nitrate is influenced by many factors, including temperature, dissolved oxygen, and hydraulic residence time (HRT). The objective of this study was to conduct a techno-economic analysis (TEA) for four scales of woodchip denitrification bioreactors operating at three HRTs (2, 8, and 16 h), designed with bypass flow or with a low probability of bypass flow, to determine the cost to remove 1 kg of NO3-N at each bioreactor scale and at each HRT. Several assumptions were made: the flow rate required to achieve a 2 h HRT on a per m3 basis could be achieved at all scales, the same mass removal of NO3-N was achieved on a per cubic meter basis, and the 2 h HRT did not have any bypass flow at each scale. With these assumptions, the lowest unit cost was observed for the large-scale bioreactor sized to have a low probability of bypass flow at 16 h HRT, with a resulting cost of $0.74 kg-1 NO3-N removed. The highest unit cost was observed for the pilot-scale bioreactor designed with bypass flow to achieve a 16 h HRT at a cost of $60.13 kg-1 NO3-N removed. At longer HRTs with bypass flow, a greater percent removal of nitrate has been observed with a lower mass removal rate. By having a low probability of bypass flow in the design, a higher mass removal and percent removal of nitrate were observed, leading to the above results. Contrasting this trend, the total and annual costs were highest for the large-scale bioreactor and lowest for the pilot-scale bioreactor. However, it was determined that 783%, 280%, and 54% increases in total cost for the pilot-, small-, and medium-scale bioreactors would be incurred to implement the number of bioreactors (66, 24, and 4, respectively) required to treat the same volume of flow as one large bioreactor. These results can be used to inform future design decisions and inform stakeholders of the approximate unit cost of installing a denitrifying woodchip bioreactor over a range of expected field conditions. While a larger bioreactor with a low probability of bypass flow may represent a more cost-effective investment, the potential for unintended, negative byproducts needs to be considered in the design. Keywords: Denitrification, Nitrate, Tile drainage, Water quality, Woodchip bioreactor.
3
Jeyakumar, Lordwin Girish Kumar, David B. McKenzie, Laura E. Christianson, and Evan Derdall. "Initial Validation of a Replicated Field-scale Denitrifying Bioreactor Facility in a Boreal Environment." Advances in Environmental and Engineering Research 02, no. 02 (December 29, 2020): 1. http://dx.doi.org/10.21926/aeer.2102005.
Abstract:
Denitrifying bioreactor technology, where a solid carbon source (woodchips) acts as a reactive medium to intercept agricultural tile drainage water, has been successfully used to convert N (NO3-) to di-nitrogen (N2) gas. Four replicated field-scale (24 m long x 3 m wide x 1 m deep), bioreactors were built and operated at the St. John’s Research and Development Centre and were successful at removing a notable amount of nitrate (N) from agricultural subsurface drainage water. The objective of this study was to investigate the internal flow dynamics of one of these field-scale bioreactors as a proxy for the others. The hydraulic conditions in the bioreactor system developed differently than expected; asymmetric flow rates led to long average hydraulic retention time (HRT) and a highly dispersed residence time distribution, which was revealed by a sodium chloride tracer test. To measure the internal flow a known amount of sodium chloride (salt) was added to water before it entered the bioreactor and samples were collected in 30 minutes intervals. The temperature of water samples taken from the inlet, outlet, and sample ports ranged from 14.5 to 18.4°C With a N removal of 62 to 66% the bioreactor proved at the same time to be very effective under the boreal environment of Newfoundland and Labrador (NL). Mass removal rate (MRR) was calculated to evaluate the performance of woodchip bioreactor. The average MRR was 3.87 gm-3day-1 and the highest was 7.19 gm-3day-1 respectively. The theoretical retention time was calculated to be approximately 10.64 h based on the active flow volume, the length and depth of the system. In comparison the observed retention was 18.18 h
4
Malhotra, Neeraj. "Bioreactors Design, Types, Influencing Factors and Potential Application in Dentistry. A Literature Review." Current Stem Cell Research & Therapy 14, no. 4 (May 23, 2019): 351–66. http://dx.doi.org/10.2174/1574888x14666190111105504.
Abstract:
Objectives:A variety of bioreactors and related approaches have been applied to dental tissues as their use has become more essential in the field of regenerative dentistry and dental tissue engineering. The review discusses the various types of bioreactors and their potential application in dentistry.Methods:Review of the literature was conducted using keywords (and MeSH) like Bioreactor, Regenerative Dentistry, Fourth Factor, Stem Cells, etc., from the journals published in English. All the searched abstracts, published in indexed journals were read and reviewed to further refine the list of included articles. Based on the relevance of abstracts pertaining to the manuscript, full-text articles were assessed.Results:Bioreactors provide a prerequisite platform to create, test, and validate the biomaterials and techniques proposed for dental tissue regeneration. Flow perfusion, rotational, spinner-flask, strain and customize-combined bioreactors have been applied for the regeneration of bone, periodontal ligament, gingiva, cementum, oral mucosa, temporomandibular joint and vascular tissues. Customized bioreactors can support cellular/biofilm growth as well as apply cyclic loading. Center of disease control & dip-flow biofilm-reactors and micro-bioreactor have been used to evaluate the biological properties of dental biomaterials, their performance assessment and interaction with biofilms. Few case reports have also applied the concept of in vivo bioreactor for the repair of musculoskeletal defects and used customdesigned bioreactor (Aastrom) to repair the defects of cleft-palate.Conclusions:Bioreactors provide a sterile simulated environment to support cellular differentiation for oro-dental regenerative applications. Also, bioreactors like, customized bioreactors for cyclic loading, biofilm reactors (CDC & drip-flow), and micro-bioreactor, can assess biological responses of dental biomaterials by simultaneously supporting cellular or biofilm growth and application of cyclic stresses.
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Wickramarathne, Niranga M., Richard A. Cooke, Ruth Book, and Laura E. Christianson. "Denitrifying Woodchip Bioreactor Leachate Tannic Acid and True Color: Lab and Field Studies." Transactions of the ASABE 63, no. 6 (2020): 1747–57. http://dx.doi.org/10.13031/trans.14020.
Abstract:
HighlightsOak leached more tannic acid, true color, and chemical oxygen demand (COD) than ash and mixed hardwood chips.The factors became similar (tannic acid, COD) or below stream levels (true color) after flushing.Eleven site-years of field bioreactor data showed decreasing tannic acid and true color over time.Post-startup tannic acid was lower in bioreactor outflow than in area streams.True color did not appear to be a reliable indicator of leachate tannic acid at low concentrations.Abstract. Woodchips have been a preferred denitrifying bioreactor medium to date, but concerns about potential harmful effects of tannins in the leachate have precluded the use of oak chips in many installations. A study was conducted to compare the suitability of oak (genus Quercus) woodchips as a denitrifying bioreactor medium relative to other types of woodchips, both in lab leachate tests and in the context of observed bioreactor leaching in the field. Assessment measures included the content of tannic acid and other compounds in the leachate, as well as leachate color, which can often be high during startup. An 84-day leaching test using rectangular bioreactor cells filled with either oak (Quercus rubra), ash (Fraxinus spp. L.), or a generic hardwood blend showed that oak initially leached higher concentrations of tannic acid, true color, and chemical oxygen demand (COD) than the other two media. The significant differences in leached concentrations among the three wood types were eliminated after a finite leaching period. Tannic acid and true color in 11 site-years of field bioreactor outflow data generally decreased over time, except following a dry period when one of the bioreactors received no drainage inflow for more than two months. The lab and field results indicated the capability of woodchip bioreactors to flush at least these two analytes to ambient stream levels. True color did not appear to be the best parameter for estimating the tannin content of woodchip leachate due to discrepancies at low concentrations. Mass normalized tannic acid leaching ranged from 0.03 to approximately 40 mg tannic acid g-1 woodchip across the lab and field assessments. Oak initially leached more tannic acid, color, and COD than the other wood types, but the eventual similarity among the wood types after flushing with a sufficient number of pore volumes meant that any potentially negative environmental impacts would likely be limited to the startup period or possibly after dry periods. Oak initially eluted higher mean total nitrogen (TN) concentrations than the other wood types, but the treatments were not significantly different by day 3, indicating that biological N removal was not significantly inhibited, even with high concentrations of tannic acid. Keywords: Chemical oxygen demand, Oak, Tannin, Water quality, Wood leachate.
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Zablodskiy, M., P. Klendiy, O. Dudar, and I. Radko. "Research of the Influence of the Combined Electromagnetic Field on Biogas Output." Problems of the Regional Energetics, no. 2(58) (May 2023): 81–96. http://dx.doi.org/10.52254/1857-0070.2023.2-58-08.
Abstract:
The purpose of research is determining the conditions of stimulating effect of the combined influence of constant and variable electromagnetic fields on the substrate and microorganisms in the bioreactor. This goal is achieved by solving the following tasks: development of mathematical model, conducting numerical simulation to determine the distribution of magnetic field in active zones of the stator-bioreactor system; conducting experimental researches during the fermentation of pig’s manure with litter from wheat straw in the mesophilic mode of fermentation. One category of bioreactors (control samples) was not exposed to influence of magnetic field, for the other, periodically were made treatment simultaneously with a low-frequency electromagnetic field and constant magnetic field synchronously with the process of mixing the substrate. The most significant results are: an experimental proof of effectiveness of the proposed method of intensification of the biogas output and increasing its quality, high accuracy of mathematical model of distribution the magnetic field in active zones of the stator-bioreactor system; assessment of the levels of consumption of nutrients by microorganisms from the substrate under the influence of the combined magnetic field and without influence of the magnetic field. The significance of obtained results lies in the fact that the proposed approach to intensification of the biogas output provides increase of the level production, the quality of biogas, and cumulative rate of methane output per unit of organic mass in the reactor.
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Kuyukina, Maria S., Anastasiya V. Krivoruchko, and Irena B. Ivshina. "Advanced Bioreactor Treatments of Hydrocarbon-Containing Wastewater." Applied Sciences 10, no. 3 (January 24, 2020): 831. http://dx.doi.org/10.3390/app10030831.
Abstract:
This review discusses bioreactor-based methods for industrial hydrocarbon-containing wastewater treatment using different (e.g., stirred-tank, membrane, packed-bed and fluidized-bed) constructions. Aerobic, anaerobic and hybrid bioreactors are becoming increasingly popular in the field of oily wastewater treatment, while high concentrations of petroleum hydrocarbons usually require physico-chemical pre-treatments. Most efficient bioreactor techniques employ immobilized cultures of hydrocarbon-oxidizing microorganisms, either defined consortia or mixed natural populations. Some advantages of fluidized-bed bioreactors over other types of reactors are shown, such as large biofilm–liquid interfacial area, high immobilized biomass concentration and improved mass transfer characteristics. Several limitations, including low nutrient content and the presence of heavy metals or toxicants, as well as fouling and contamination with nuisance microorganisms, can be overcome using effective inocula and advanced bioreactor designs. The examples of laboratory studies and few successful pilot/full-scale applications are given relating to the biotreatment of oilfield wastewater, fuel-contaminated water and refinery effluents.
8
Sassi, Lisa, Omolola Ajayi, Sara Campinoti, Dipa Natarajan, Claire McQuitty, Riccardo Rayan Siena, Sara Mantero, et al. "A Perfusion Bioreactor for Longitudinal Monitoring of Bioengineered Liver Constructs." Nanomaterials 11, no. 2 (January 21, 2021): 275. http://dx.doi.org/10.3390/nano11020275.
Abstract:
In the field of in vitro liver disease models, decellularised organ scaffolds maintain the original biomechanical and biological properties of the extracellular matrix and are established supports for in vitro cell culture. However, tissue engineering approaches based on whole organ decellularized scaffolds are hampered by the scarcity of appropriate bioreactors that provide controlled 3D culture conditions. Novel specific bioreactors are needed to support long-term culture of bioengineered constructs allowing non-invasive longitudinal monitoring. Here, we designed and validated a specific bioreactor for long-term 3D culture of whole liver constructs. Whole liver scaffolds were generated by perfusion decellularisation of rat livers. Scaffolds were seeded with Luc+HepG2 and primary human hepatocytes and cultured in static or dynamic conditions using the custom-made bioreactor. The bioreactor included a syringe pump, for continuous unidirectional flow, and a circuit built to allow non-invasive monitoring of culture parameters and media sampling. The bioreactor allowed non-invasive analysis of cell viability, distribution, and function of Luc+HepG2-bioengineered livers cultured for up to 11 days. Constructs cultured in dynamic conditions in the bioreactor showed significantly higher cell viability, measured with bioluminescence, distribution, and functionality (determined by albumin production and expression of CYP enzymes) in comparison to static culture conditions. Finally, our bioreactor supports primary human hepatocyte viability and function for up to 30 days, when seeded in the whole liver scaffolds. Overall, our novel bioreactor is capable of supporting cell survival and metabolism and is suitable for liver tissue engineering for the development of 3D liver disease models.
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Grün, Christoph, Brigitte Altmann, and Eric Gottwald. "Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part I: A Systematic Analysis of the Literature Published between 2000 and 2020." Processes 8, no. 12 (December 15, 2020): 1656. http://dx.doi.org/10.3390/pr8121656.
Abstract:
Bioreactors have proven useful for a vast amount of applications. Besides classical large-scale bioreactors and fermenters for prokaryotic and eukaryotic organisms, micro-bioreactors, as specialized bioreactor systems, have become an invaluable tool for mammalian 3D cell cultures. In this systematic review we analyze the literature in the field of eukaryotic 3D cell culture in micro-bioreactors within the last 20 years. For this, we define complexity levels with regard to the cellular 3D microenvironment concerning cell–matrix-contact, cell–cell-contact and the number of different cell types present at the same time. Moreover, we examine the data with regard to the micro-bioreactor design including mode of cell stimulation/nutrient supply and materials used for the micro-bioreactors, the corresponding 3D cell culture techniques and the related cellular microenvironment, the cell types and in vitro models used. As a data source we used the National Library of Medicine and analyzed the studies published from 2000 to 2020.
10
Christianson, Laura Elizabeth, Reid Christianson, Carolina Díaz-García, Gabriel Johnson, Bryan Maxwell, Richard Andrew Cooke, N. M. Wickramarathne, and Lowell Gentry. "Denitrifying Bioreactor In Situ Woodchip Bulk Density." Journal of the ASABE 66, no. 3 (2023): 723–34. http://dx.doi.org/10.13031/ja.15364.
Abstract:
Highlights The bulk density of woodchips in denitrifying bioreactors in the field is unknown. In situ bulk density estimation methods were developed for use during construction or excavation. Dry bulk densities of aged woodchips at bioreactor bottoms were lower than previous literature values. Moisture and particle size and density explained some, but not all, of the variation in in situ bulk densities. Abstract. Woodchip bulk density in a denitrifying bioreactor governs system hydraulics, but this prime physical attribute has never been estimated in situ. The objectives were twofold: (1) to establish estimates of in situ woodchip bulk density at bioreactors in the field, and (2) evaluate causal factors for and resulting impacts of these estimates. Proof-of-concept bulk density methods were developed at a pilot-scale bioreactor using three ways to estimate volume: surveying the excavated area, pumping the excavation full through a flow meter, and using iPhone Light Detection and Ranging (LiDAR). These methods were then further tested at two new and three old full-size bioreactors. Additional ex situ (off-site) testing with the associated woodchips included analysis of bulk density along a moisture gradient and particle size, particle density, wood composition, and hydraulic property testing. In situ dry bulk densities based on the entire volume of the new bioreactors (206-224 kg/m3) were similar to values from previous lab-scale studies. In situ estimates for woodchips at the bottom of aged bioreactors (22-mo. to 6-y) were unexpectedly low (120-166 kg/m3), given that these woodchips would presumably be the most compacted. These low moisture-content corrected dry bulk densities were influenced by high moisture contents in situ (>70% wet basis). The impacts of particle size and particle density on bulk density were somewhat mixed across the dataset, but in general, smaller woodchips had higher dry bulk densities than larger, and several woodchips sourced from the bottom of bioreactors had low particle densities. Although dry bulk densities in the zone of flow in bioreactors in the field were shown to be relatively low, the resulting permeability coefficients under those packing conditions did not differ from those of the original woodchips. The LiDAR-based volume estimation method was the most practical for large-scale, full-size evaluations and allowed high precision with small features (e.g., vertical reactor edges, drainage fittings). Keywords: Compaction, Cone penetrometer, Drainable porosity, LiDAR, Moisture content, Survey.
Dissertations / Theses on the topic "Field bioreactor":
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Jain, Pradeep. "Moisture addition at bioreactor landfills using vertical wells mathematical modeling and field application /." [Gainesville, Fla.] : University of Florida, 2005. http://purl.fcla.edu/fcla/etd/UFE0010860.
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ZEIN, MAHER M. "MTBE BIODEGRADATION IN AN INNOVATIVE BIOMASS CONCENTRATOR REACTOR: THE EVOLUTION FROM LABORATORY TO FIELD APPLICATION." University of Cincinnati / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1140534772.
3
Vaidya, Rajendra D. "Solid Waste Degradation, Compaction and Water Holding Capacity." Thesis, Virginia Tech, 2002. http://hdl.handle.net/10919/35432.
Abstract:
Bioreactor landfills offer a sustainable way to achieve increased waste degradation along with benefits such as enhanced landfill gas (LFG) recovery, reduction in leachate pollution potential and rapid increase in landfill volumetric capacity. It also offers significant reduction in post closure management activities as leachate treatment, LFG impact on the environment and improves the potential for land reuse. The regulatory 30 year post-closure period is believed to account for attenuation of organics, metals and trace pollutants of adverse environmental consequences. Methodologies to improve the degradation rate and process are refuse shredding, nutrient addition, pH buffering, and temperature control along with moisture enhancement. Municipal Solid Waste (MSW) settlement and field capacity are of significant beneficial interest to achieve maximum utility of landfill volume and compute water requirements for rapid degradation using bioreactor concepts.
Physical and biochemical Municipal Solid Waste (MSW) characteristics were investigated with specific emphasis on the Bio-Chemical methane potential (BMP) test. The impact of waste characteristics on its compressibility and moisture retention capacity was evaluated on a laboratory scale. Traditional in-situ waste compression models from literature were used to compare with the obtained laboratory data.Master of Science
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Walters, Evan Robert. "Sulfate Reducing Bioreactor Dependence on Organic Substrates for Long-Term Remediation of Acid Mine Drainage." OpenSIUC, 2014. https://opensiuc.lib.siu.edu/theses/1397.
Abstract:
Coal-generated acid mine drainage (AMD) is characterized by low-pH waters with excessive loads of dissolved species such as SO4, Fe, Al and Mn along with other elements of environmental concern (i.e. Cd, As, Cr, Ni, Pb, Se and Cu). To mitigate this problem, anaerobic sulfate reducing bioreactors (ASRB) have been implemented as a technology for passive treatment systems that utilize low-cost organic substrates to stimulate biologically enhanced contaminant sequestration. Previous work has identified the establishment of diverse microbial communities in which a hierarchal chain of substrate degradation processes is essential in developing sustainable environments to produce long-lived sulfate-reducing microbial (SRM) populations. In this study, to determine the optimal mixture of substrate types, alternating ratios of herbaceous (ie. leaves, grass, spent brewing grains) and ligneous (i.e. maple wood chips and saw dust) reactor matrices were tested. Five bioreactors along with one control reactor containing only limestone were constructed at the Tab-Simco abandoned mine land (AML) site in southern Illinois, USA. The field experiments were monitored over ~ one year (377 days) to evaluate the physical, geochemical and microbiological parameters which dictate ASRB efficiency in remediation of AMD contaminants. Results from this experiment documented contaminant removal in all reactors. However, the bioreactors established SRM populations that contributed to enhanced removal of SO4, Fe, and trace metals (i.e. Cu, Cd, Zn, Ni). Geochemical assessment of the aqueous environments established within the bioreactors suggested multiple pathways of contaminant sequestration. This included the formation of Fe-oxyhydroxide precipitates, adsorption, co-precipitation (e.g. Zn/Ni-Ferrites) and bio-induced sulfide mineralization. Activity of the SRMs was dependent on temperature, with bioreactors exhibiting decreases in both effluent sulfide concentrations and 34S-depletion of sulfate during low-T months (i.e. T < 10°C). Overall, maximum remediation of dissolved constituents SO4, Fe, Al and Mn was obtained in the predominantly herbaceous bioreactors. Extrapolation of our results to the full-scale Tab-Simco bioreactor indicated that, over the course of one year, the herbaceous bioreactors would remove ~75,600 kg SO4, 21,800 kg Fe, 8000 kg Al, and 77 kg Mn. This represents a 21.7 wt%, 41.5 wt%, 9.4 wt% and 81.8 wt% increase in SO4, Fe, Al and Mn removal over dominantly ligneous bioreactors, respectively. Although the overall Fe removal within the limestone control reactor reached 44.5 mol%; removal of 19.5 mol% SO4 and 36.9 mol% Al from influent AMD were significantly less when compared to the bioreactors. These results imply that ASRB technologies are promising in remediation of coal-generated AMD and increasing herbaceous content of bioreactors can significantly enhance contaminant sequestration. However, geochemical results also displayed seasonal variation in redox gradients within our field ASRB's which may induce dissolution of the redox sensitive phases produced within bioreactors. Furthermore, optimal microbial-mediated sulfate reduction may be inhibited by the high surface areas of the abundant Fe/Al-oxyhydroxides which dominate the system. Therefore, to enhance ASRB remediation capacity, future designs must optimize not only the organic carbon substrate but also include a pretreatment phase in which the bulk of dissolved Fe/Al-species are removed from the influent AMD prior to entering the bioreactor.
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Dikina, Anna D. "ENGINEERED CARTILAGE COMPOSED OF MESENCHYMAL STEM CELL CONDENSATES AS MODULES WITH CONTROLLED SHAPE AND SIZE FOR MULTI-TISSUE TYPE CONSTRUCTS, AS MATERIALS FOR CHONDROCONDUCTIVE SCAFFOLDS AND AS MECHANORESPONSIVE TISSUES." Case Western Reserve University School of Graduate Studies / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=case1459254069.
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Setford, S. J. "Combined bioreaction and separation in centrifugal fields." Thesis, Aston University, 1992. http://publications.aston.ac.uk/9781/.
Abstract:
The aim of this work has been to investigate the principle of combined centrifugal bioreaction-separation. The production of dextran and fructose by the action of the enzyme dextransucrase on sucrose was employed to elucidate some of the principles of this type of process. Dextran is a valuable pharmaceutical product used mainly as a blood volume expander and blood flow improver whilst fructose is an important dietary product. The development of a single step process capable of the simultaneous biosynthesis of dextran and the separation of the fructose by-product should improve dextran yields whilst reducing capital and processing costs. This thesis shows for the first time that it is possible to conduct successful bioreaction-separations using a rate-zonal centrifugation technique. By layering thin zones of dextrasucrase enzyme onto sucrose gradients and centrifuging, very high molecular weight (MW) dextran-enzyme complexes were formed that rapidly sedimented through the sucrose substrate gradients under the influence of the applied centrifugal field. The low MW fructose by-product sedimented at reduced rates and was thus separated from the enzyme and dextran during the reaction. The MW distribution of dextran recovered from the centrifugal bioreactor was compared with that from a conventional batch bioreactor. The results indicated that the centrifugal bioreactor produced up to 100% more clinical dextran with MWs of between 12 000 and 98 000 at 20% w/w sucrose concentrations than conventional bioreactors. This was due to the removal of acceptor fructose molecules from the sedimenting reaction zone by the action of the centrifugal field. Higher proportions of unwanted lower MW dextran were found in the conventional bioreactor than in the centrifugal bioreactor-separator. The process was studied on a number of alternative centrifugal systems. A zonal rotor fitted with a reorienting gradient core proved most successful for the evaluation of bioreactor performance. Results indicated that viscosity build-up in the reactor must be minimised in order to increase the yields of dextran per unit time and improve product separation.
Books on the topic "Field bioreactor":
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Setford, Steven John. Combined bioreaction and separation in centrifugal fields. Birmingham: Aston University. Department of Chemical Engineering and Applied Chemistry, 1992.
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Douglas, Kenneth. Bioprinting. Oxford University Press, 2021. http://dx.doi.org/10.1093/oso/9780190943547.001.0001.
Abstract:
Abstract: This book describes how bioprinting emerged from 3D printing and details the accomplishments and challenges in bioprinting tissues of cartilage, skin, bone, muscle, neuromuscular junctions, liver, heart, lung, and kidney. It explains how scientists are attempting to provide these bioprinted tissues with a blood supply and the ability to carry nerve signals so that the tissues might be used for transplantation into persons with diseased or damaged organs. The book presents all the common terms in the bioprinting field and clarifies their meaning using plain language. Readers will learn about bioink—a bioprinting material containing living cells and supportive biomaterials. In addition, readers will become at ease with concepts such as fugitive inks (sacrificial inks used to make channels for blood flow), extracellular matrices (the biological environment surrounding cells), decellularization (the process of isolating cells from their native environment), hydrogels (water-based substances that can substitute for the extracellular matrix), rheology (the flow properties of a bioink), and bioreactors (containers to provide the environment cells need to thrive and multiply). Further vocabulary that will become familiar includes diffusion (passive movement of oxygen and nutrients from regions of high concentration to regions of low concentration), stem cells (cells with the potential to develop into different bodily cell types), progenitor cells (early descendants of stem cells), gene expression (the process by which proteins develop from instructions in our DNA), and growth factors (substances—often proteins—that stimulate cell growth, proliferation, and differentiation). The book contains an extensive glossary for quick reference.
Book chapters on the topic "Field bioreactor":
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Söndahl, M. R., C. N. Söndahl, and W. Goncalves. "Field Testing of Arabica Bioreactor-Derived Plants." In Coffee Biotechnology and Quality, 143–50. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-1068-8_11.
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van den Munckhof, Ger P. M., and Martin F. X. Veul. "Production-scale trials on the decontamination of oil-polluted soil in a rotating bioreactor at field capacity." In Contaminated Soil ’90, 993–95. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-011-3270-1_215.
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Gokul Varshan, M., P. Joel Josephson, Bijaya Bijeta Nayak, Venkatesan Hariram, and K. Balachandar. "History, Challenges, and Opportunities in Tissue Engineering." In Handbook of Research on Advanced Functional Materials for Orthopedic Applications, 148–67. IGI Global, 2023. http://dx.doi.org/10.4018/978-1-6684-7412-9.ch009.
Abstract:
In the last several decades, the area of tissue engineering has experienced significant growth, bringing to the clinic treatments that were long thought to be the stuff of science fiction. Although the use of tissue engineering concepts in clinics is not particularly common, the field is predicted to have a very bright future as more tissues will be added to the list of “clinically applicable tissue engineered constructs.” Future advancements are likely to make it feasible to combine immune-transparent cells with a commercially available scaffold and cultivate them in a sophisticated bioreactor to provide messages specifically designed for the target region. However, much basic and applied scientific study is still needed before off-the-shelf body parts become therapeutically useful. The development of innovative biomaterials for the various tissue engineering and regenerative medicine applications will be the main focus of future efforts. The biomaterials' structure and mechanical characteristics will be tailored to better fit the target tissue.
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Tiwari, Deepesh, Athar Hussain, Sunil Kumar Tiwari, Salman Ahmed, Mohd Wajahat Sultan, and Mohd Imran Ahamed. "Removal of Heavy Metals using Microbial Bioremediation." In Bioremediation for Environmental Pollutants, 42–64. BENTHAM SCIENCE PUBLISHERS, 2023. http://dx.doi.org/10.2174/9789815123494123010004.
Abstract:
The unorganized dumping of effluents along with different wastes directly into the water and soil has resulted in the rise of the concentration of many harmful metals, chemicals, and other gases in the environment. Widely known heavy metals triggering pollution issues are Lead (Pb), Chromium (Cr), Mercury (Hg), Cadmium (Cd), Copper (Cu), Arsenic (As) and Selenium (Se), as these heavy metals are generally found in the effluents of fertilizers, metallurgy, electroplating, and electronics industries. A number of physical-chemical reactions such as acid-base, oxidation-reducing, precipitation- dissolution, solubilization and ion-exchange processes occur and affect metal speciation. The physical methods used for heavy metals removal include magnetic separation, electrostatic separation, mechanical screening method, hydrodynamic classification, gravity concentration, flotation, and attrition scrubbing. The chemical methods used for eliminating heavy metals are chemical precipitation, coagulation and flocculation processes and the heavy metals are therefore removed as sludge. Electro-deposition, membrane filtration, electro-flotation and electrical oxidation are the various electrochemical treatment methods that are used to remove heavy metals from wastewater. Bioremediation is a biological method of eliminating toxins from the environment by using biological microbial bacteria such as Pseudomonas and Sphingomonas. Examples of bioremediation technologies include field farming, bioleaching, phytoremediation, bioventing, bioreactor, bio-stimulation and composting. Bioremediation is a natural process and is quite applicable as a waste treatment process for contaminated soils. The microbes present in the solution or soil can degrade the pollutants. It can also prove to be less expensive than other technologies that are used for clean-up of hazardous waste and are also useful for the destruction of a wide variety of contaminants as many hazardous compounds can be transformed into harmless products.<br>
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Shareefdeen, Zarook. "Industrial Biofilter Design for Removal of Hydrogen Sulphide (H2S) from Wastewater Treatment Plants." In Sustainability Studies: Environmental and Energy Management, 166–80. BENTHAM SCIENCE PUBLISHERS, 2022. http://dx.doi.org/10.2174/9789815039924122010011.
Abstract:
Hydrogen sulphide (H2S) is the main odor-causing, toxic, and corrosive chemical found in wastewater treatment operations. Bio-oxidation based processes for air pollutant removal have become more attractive to the industry and numerous wastewater treatment facilities have replaced conventional air treatment technologies such as adsorption and chemical scrubbing with bio-oxidation based processes such as biofilters. Of the three main types of air phase bioreactors, biofilter is used more commonly than the others due to its simple configuration, ease of operation, and economic benefits. This chapter addresses challenges in the industrial biofilter design for H2S removal from wastewater treatment facilities. Wastewater industry professionals, biofilter customers, biofilter vendors, and researchers who work in the field of odor and H2S emission control and biofilter design will find this chapter very useful.
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Cuyper, Marcel De. "Applications of Magnetoproteoliposomes in Bioreactors Operating in High-Gradient Magnetic Fields." In Handbook of Nonmedical Applications of Liposomes, 325–42. CRC Press, 2018. http://dx.doi.org/10.1201/9781351072724-20.
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Pereira, Julianne Soares, Keyla Nunes Farias Gomes, Caroline de Souza Ferreira Pereira, Geovana Espíndola Jardim, Patrícia Soares Santiago, Leandro Machado Rocha, and Robson Xavier Faria. "Microalgae and the medicine of the future." In Health of Tomorrow: Innovations and Academic Research. Seven Editora, 2023. http://dx.doi.org/10.56238/sevened2023.007-001.
Abstract:
This chapter looks at the biotechnological potential of microalgae and their promising use in medicine. We explore their applications as bioreactors in producing pharmaceutical substances and their innovative capacity in gene therapy. In addition, we highlight the growing interest in their nutritional properties, especially omega-3 fatty acids, and high-quality antioxidants. Our analysis also showed the impact of microalgae in the field of vaccines and immunotherapies, highlighting their ability to improve the efficacy of vaccines and drive the development of advanced immunization methods. We discussed current challenges and prospects in the broader context, highlighting the continued need for in-depth research and investment in this emerging area. This chapter has aimed to provide a comprehensive overview of the transformative potential of microalgae in medicine, emphasizing their role as one of the fundamental pillars for the continued advancement of human health and the development of innovative therapies on the horizon of future medicine. Hopefully, this analysis will stimulate further discussion and inspire new research, thus driving progress biomedical research.
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Aide, Michael, Indi Braden, and Sven Svenson. "Edge of Field Technology to Eliminate Nutrient Transport from Croplands: Specific Focus on Denitrification Bioreactors." In Soil Contamination - Current Consequences and Further Solutions. InTech, 2016. http://dx.doi.org/10.5772/64602.
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Mitra, Monisha, Himanshi Swain, Oindrila Debsarma, and Nirmal Mandal. "BIOTECHNOLOGICAL INTERVENTIONS IN UPSCALING OF PLANT SECONDARY METABOLITES." In Futuristic Trends in Biotechnology Volume 2 Book 26, 245–62. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2023. http://dx.doi.org/10.58532/v2bs26ch15.
Abstract:
Plants are considered as a great hub of secondary metabolites with high value that have uses in a variety of fields. Whenever the natural source is insufficient or chemical synthesis is not viable, plant tissue culture methods are regarded as viable and eco -friendly for the smooth production of secondary metabolites. The main benefits of using plant tissue culture techniques for the production and enrichment of plant secondary metabolites are discussed in this chapter, along with the various biotechnological methods that can be used to upscale their production. The chapter demonstrates that although there are several instances describing the synthesis of differentiated cells and tissues especially hairy roots and undifferentiated cells are the ideal culture method employed for the creation of valuable secondary metabolites under in vitro conditions. The potential ways to improve the biosynthesis of valuable compounds produced by any plant in vitro systems are outlined in an integrated manner. This includes metabolic engineering, which regulates plant metabolism by overexpressing/repressing a single structural gene or a group of structural genes. The production of secondary metabolites from plant origin at laboratory or industrial scales, various bioreactor system types, their modification, and ideal process parameters are described
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Eswaran, Vishal, Ushaa Eswaran, Vivek Eswaran, and Keerthna Murali. "Bioelectronics and Application in Waste Treatment Systems for Smart City." In Revolutionizing Automated Waste Treatment Systems, 195–220. IGI Global, 2024. http://dx.doi.org/10.4018/979-8-3693-6016-3.ch013.
Abstract:
The rapid urbanization of modern societies has presented cities with numerous challenges, including the effective management of waste streams. In response, the concept of smart cities has emerged, leveraging cutting-edge technologies to address these complexities sustainably. This chapter explores the integration of bioelectronics, an interdisciplinary field combining principles from biology and electronics, into waste treatment systems tailored for smart cities. The chapter begins by introducing the fundamental concepts and principles underlying bioelectronics, including biosensors, biofuel cells, neural implants, and wearable health monitors. It then delves into the challenges faced by conventional centralized waste treatment systems, such as inefficiency, resource depletion, and environmental pollution, highlighting the need for alternative approaches. The integration of bioelectronic devices like microbial fuel cells, biosensors, and bioreactors into waste treatment systems is proposed as a transformative solution, offering real-time monitoring, precise control, and enhanced pollutant degradation capabilities. Case studies and examples are provided to demonstrate the efficacy of these technologies in wastewater treatment, pollutant detection, and environmental risk mitigation. While acknowledging the potential benefits, the chapter also addresses the challenges associated with the integration of bioelectronics into waste treatment systems, including scalability, cost-effectiveness, and regulatory compliance. Strategies for overcoming these challenges, such as interdisciplinary collaboration, technological innovation, and policy support, are discussed. The chapter concludes by emphasizing the transformative potential of bioelectronics in revolutionizing waste treatment systems for smart cities, enabling increased efficiency, sustainability, and resilience, while promoting circular economy principles and environmental stewardship.
Conference papers on the topic "Field bioreactor":
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Foye, K. C., X. Zhao, T. C. Voice, and S. A. Hashsham. "Settlement Monitoring for Bioreactor Landfill Airspace Management." In Seventh International Symposium on Field Measurements in Geomechanics. Reston, VA: American Society of Civil Engineers, 2007. http://dx.doi.org/10.1061/40940(307)40.
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Yang, Gang, Haiyan Long, Jiang Wu, and Hua Huang. "A Novel Electrical Field Bioreactor for Wound Healing Study." In 2008 International Conference on Biomedical Engineering And Informatics (BMEI). IEEE, 2008. http://dx.doi.org/10.1109/bmei.2008.130.
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Korin, Natanel, Avishay Bransky, Uri Dinnar, and Shulamit Levenberg. "Modeling and Studying Human Embryonic Stem Cell Culture Conditions in Pulsed Flow Micro-Reactors." In ASME 2008 9th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2008. http://dx.doi.org/10.1115/esda2008-59168.
Abstract:
Embryonic stem (ES) cells research is a promising field for tissue engineering due to their proliferative capacity and differentiation abilities. The culture of Human Embryonic Stem Cells (hESC) in microchannel bioreactors can be valuable for hESC cell biology studies and hESC tissue engineering applications. We have previously demonstrated the long-term culture of mammalian (HFF-Human Foreskin Fibroblasts) cells in a microchannel (130μm) bioreactor under constant perfusion in a simple approach. However, hESC were found to be highly sensitive to flow and did not grow under flow rates which were proper for HFF long-term culture. Here, we propose the use of a novel automated periodic perfusion system to co-culture hESC with HFF in a microchannel bioreactor. The method is based on short temporal pulsed flows of medium renewal followed by long static incubation periods. The short pulsed exposure to shear enables shear sensitive cells (e.g., hESC) to withstand the medium flow. The present work studies experimentally and via numerical simulations the conditions required for hESC culture in a microchannel bioreactor using the periodic perfusion method. Conventional soft-lithography techniques were used to fabricate PDMS microchannels (100 μm) sealed reversibly with glass cover slides. HESC were seeded in the microchannel with HFF, incubated for several hours and then connected to a perfusion system which contained: a syringe pump, a permeable tube oxygenator, and waste container. The ability of the periodic perfusion protocols to prevent hESC de-attachment and maintain their culture was examined. Mass transport and fluid mechanics models were used to evaluate the culture conditions within the micro-bioreactor (shear stress, oxygen level, nutritious etc.). 3D finite element mass transport analysis (Comsol 3.3) was preformed to examine the oxygen levels in the microchannel as a function of time and design parameters. Altogether, the experimental results and the theoretical model indicate that the use of a periodic perfusion bioreactor is a suitable and promising method to culture hESC in a microreactor. Culturing undifferentiated human ES cell colonies in a micro-bioreactor is an initial step toward utilizing microfluidic techniques to investigate embryonic stem cell biology.
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Straume, Indulis, Imants Plume, Vilis Dubrovskis, Viktors Dreimanis, and Eriks Zukovskis. "Biogas potential from co-fermentation of food leftovers and lignocellulosic biomass at mesophilic temperatures." In 22nd International Scientific Conference Engineering for Rural Development. Latvia University of Life Sciences and Technologies, Faculty of Engineering, 2023. http://dx.doi.org/10.22616/erdev.2023.22.tf081.
Abstract:
Every year, large amounts of food leftovers are thrown away in catering establishments and households. Industry and agriculture produce lignocellulosic residues, including paper dust and willow biomass, which cause environmental problems if not properly disposed of. The aim of this study is to investigate the biogas and biomethane yields of these biomasses during anaerobic co-fermentation under mesophilic conditions. Biogas yields were determined by co-fermentation of food (hospital canteen, cafeteria, and household) residues and lignocellulosic (paper dust and shredded willow) biomass in a number of 0.72 L bioreactors. All bioreactors were divided into groups having the same content in reactors within each group to ensure the reliability of the results. Groups of bioreactors used for anaerobic fermentation were inoculums (0.5 L) only, inoculums with individual biomass, and inoculums with mixture of two or more biomass. Bioreactors were placed in three different thermostats with 16 bioreactors in each thermostat. Single fill batch anaerobic fermentation (AF) process was provided at 28, 33 and 38 °C. Individual reactor groups were equipped with graphite electrodes connected to DC voltage of 0.7 V. The biogas released in the bioreactor was collected into a gas bag outside the reactor. AF was maintained until gas emission ceased. The highest biogas yield in the AF process was obtained from the bioreactors at a temperature of 38 °C and the lowest at a temperature of 28 °C. Co-fermentation of biomass increased biogas and methane yields compared to AF treatment of individual biomasses. Exposure to the electric field decreased the methane yield. The energy balance on the AF process with the application of the electric field should be calculated by considering also the energy of hydrogen released from substrates with electrodes installed.
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Turysbekova, Gaukhar, Yerkin Bektay, Akmurat Altynbek, Muratbek Sidikanov, and Bauyrzhan Shiderin. "INFLUENCE OF IRON-OXIDIZING BACTERIA ON THE REDOX POTENTIAL OF THE LEACHING SOLUTION IN SITU RECOVERY (ISR) OF URANIUM." In 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022v/6.2/s25.27v.
Abstract:
At one of the Kazakh uranium deposits, studies were carried out on the effect of ironoxidizing bacteria on the redox potential of leaching solutions. The studies carried out on three bioreactors with a volume of 1 m3 and one bioreactor with volume 6 m3 made it possible to establish a close relationship between the content of ferric iron in the solution and the redox potential of the leaching solution. In situ recovery of uranium uses sulfuric acid leaching solutions containing sulfuric acid from 3.5 g/liter to 25 grams/liter. Diagrams of the relationship between redox and the content of a trivalent iron in solution were obtained. This makes it possible to carry out an operational analysis of the composition of the leaching solution according to the redox of the solution directly in the conditions of the geotechnological field.
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Neitzel, G. Paul, Robert M. Nerem, Athanassios Sambanis, Marc K. Smith, Timothy M. Wick, Jason B. Brown, Christopher Hunter, et al. "Effect of Fluid-Mechanical and Chemical Environments on Cell Function and Tissue Growth: Experimental and Modeling Studies." In ASME 1998 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1998. http://dx.doi.org/10.1115/imece1998-0794.
Abstract:
Abstract Bioreactors are widely used for the growth and maintenance of tissue-engineered constructs. In this paper, we report on work directed toward a better understanding of the chemical and fluid-mechanical environments that are needed to enhance cell function and tissue growth in bioreactors. We have conducted cell-growth studies in well-controlled flow conditions that indicate the effect of shear stress and oxygen tension on cellular function. In more complicated bioreactors, like the NASA rotating-wall vessel bioreactor, we have done experimental and numerical fluid-mechanical studies that quantify the velocity and shear-rate fields near a three-dimensional construct suspended by the flow inside the bioreactor. All of these results will be used to develop the tools needed to properly design and operate bioreactors for the optimal growth of tissue substitutes.
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Ferrar, Joseph, Philip Maun, Kenneth Wunch, Joseph Moore, Jana Rajan, Jon Raymond, Ethan Solomon, and Matheus Paschoalino. "High Pressure, High Temperature Bioreactors as a Biocide Selection Tool for Hydraulically Fractured Reservoirs." In SPE Hydraulic Fracturing Technology Conference and Exhibition. SPE, 2021. http://dx.doi.org/10.2118/204198-ms.
Abstract:
Abstract We report the design, operation and biogenic souring data from a first-of-its kind suite of High Pressure, High Temperature (HPHT) Bioreactors for hydraulically fractured shale reservoirs. These bioreactors vet the ability of microbial control technologies, such as biocides, to prevent the onset of microbial contamination and reservoir souring at larger experimental volumes and higher pressures and temperatures than have been previously possible outside of field trials. The bioreactors were charged with proppant, crushed Permian shale, and sterile simulated fracturing fluids (SSFF). Subsets of bioreactors were charged with SSFF dosed with either no biocide, tributyl tetradecyl phosphonium chloride (TTPC, a cationic surface-active biocide), or 4,4-dimethyloxazolidine (DMO, a preservative biocide). The bioreactors were shut in under 1,000-2,500 psi and elevated temperatures for up to fifteen weeks; hydrogen sulfide (H2S) and microbial counts were measured approximately once per week, and additional microbes were introduced after weeks three and five. Across two separate studies, the bioreactors containing no biocide soured within the first week of shut-in and H2S concentrations increased rapidly beyond the maximum detectable level (343 ppm) within the first three to six weeks of shut-in. In the first study, the bioreactors treated with TTPC soured within two weeks of shut-in (prior to the first addition of fresh microbes), and H2S concentrations increased rapidly to nearly 200 ppm H2S within the first six weeks of shut-in and beyond the maximum detectable level after fifteen weeks of shut-in. The bioreactors containing DMO did not sour during either study until at least the first addition of fresh microbes, and higher levels of the preservative biocide continued to prevent the biogenic formation of H2S even during and after the addition of fresh microbes. Microbial counts correlate with the H2S readings across all bioreactor treatments. The differentiation in antimicrobial activity afforded by the different types of biocide treatments validates the use of these simulated laboratory reservoirs as a biocide selection tool. This first-of-its-kind suite of HPHT Bioreactors for hydraulic fracturing provides the most advanced biocide selection tool developed for the hydraulic fracturing industry to date. The bioreactors will guide completions and stimulation engineers in biocide program optimization under reservoir-relevant conditions prior to beginning lengthy and expensive field trials.
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Chung, Eunna, and Marissa Nichole Rylander. "Effects of Growth Factors and Stress Conditioning on the Induction of Heat Shock Proteins and Osteogenesis." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206662.
Abstract:
Tissue engineering is an emerging field that focuses on development of methods for repairing and regenerating damaged or diseased tissue. Successful development of engineered tissues is often limited by insufficient cellular proliferation and insufficient formation of extracellular matrix. To induce effective bone regeneration, many research groups have investigated the cellular response and capability for tissue regeneration associated with bioreactor conditions and addition of growth factors [1]. Bioreactors in tissue engineering have been developed to expose cells to a similar stress environment as found within the body or induce elevated stress levels for potential induction of specific cellular responses associated with tissue regeneration. Native bone encounters a diverse array of dynamic stresses such as shear, tensile, and compression daily. Stress conditioning protocols in the form of thermal or tensile stress have been shown to induce up-regulation of molecular chaperones called heat shock proteins (HSPs) and bone-related proteins like MMP13 (matrix metallopeptidase 13) [2] and OPG (osteoprotegerin) [3, 4]. HSPs have important roles in enhancing cell proliferation and collagen synthesis. Osteogenic growth factors such as TGF-β1 (transforming growth factor beta 1) and BMP-2 (bone morphogenetic protein 2) are related to bone remodeling and osteogenesis as well as HSP induction [5]. Therefore, identification of effective preconditioning using growth factors and stress protocols that enhance HSP expression could substantially advance development of bone regeneration. The purpose of this research was to identify preconditioning protocols using osteogenic growth factors and tensile stress applied through a bioreactor system to enhance expression of HSPs and bone-related proteins while minimizing cellular injury for ultimate use for bone regeneration.
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Zablodskiy, Mykola, Petr Klendiy, and Volodymyr Gritsyuk. "The Influence of a Rotating Magnetic Field on the Intensity of Methane Formation in a Bioreactor." In 2019 IEEE 39th International Conference on Electronics and Nanotechnology (ELNANO). IEEE, 2019. http://dx.doi.org/10.1109/elnano.2019.8783810.
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Silva, Veronica, Kenneth Wunch, Ethan Solomon, and Philip Maun. "Extended Field Study Tracking the Performance of the Preservative Biocides, THNM and DMO, in Unconventional Wells." In SPE International Conference on Oilfield Chemistry. SPE, 2023. http://dx.doi.org/10.2118/213862-ms.
Abstract:
Abstract Hydraulic fracturing is prone to microbial contamination due to the large volumes of water used to recover oil and gas from the reservoir. Biocides are frequently added during hydraulic fracturing to mitigate operational problems such as souring, corrosion, and biofouling. In this multi-year case study, the preservative biocides, 4,4-dimethyloxazolidine (DMO) and tris(hydroxymethyl) nitromethane (THNM), were applied during completions for the long-term control of microbial contamination capable of surviving harsh reservoir conditions and to optimize hydrocarbon recovery. A suite of high temperature, high pressure (HPHT) bioreactors were developed to investigate the ability of biocides to prevent souring in model hydraulic fracturing systems. The above-mentioned preservative biocides, known for their endurance, were selected for inoculation into the reactors. These results were compared to an identical biocide field application in the Vaca Muerta basin in Argentina. A multi-year study was conducted measuring efficacy of the biocide program in the produced fluids by measuring ATP, qPCR, most probable number (MPN) and NGS (Next Generation Sequencing) in selected samples. Concurrently, the operator measured H2S levels in the production fluids and calculated the estimated savings in H2S scavengers top side by implementing preservative biocides. Results indicated that both DMO and THNM were able to effectively control souring and microbial growth in treated reactors at 60°C compared to the untreated control. When comparing reactor results to field applications, both biocides were successful in maintaining control of the field microbial populations and produced H2S from flowback through a few years of production. Furthermore, observations of nearby wells treated with non-preservative biocides indicated those wells lost control of the microbial population in the reservoirs after approximately 10 months of production. This microbial control study allows the correlation of lab bioreactor performance to real-world field results and demonstrates the effectiveness of the preservative biocides, DMO and THNM, in the long-term control of microbial biofouling in an unconventional reservoir.
Reports on the topic "Field bioreactor":
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Donaldson, T. L., A. J. Lucero, H. L. Jennings, and S. E. Herbes. Pilot-scale field tests for the methanotrophic technology cometabolic bioreactor demonstration at the Oak Ridge K-25 Site. Office of Scientific and Technical Information (OSTI), June 1993. http://dx.doi.org/10.2172/10176621.
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Lynn Katz, Kerry Kinney, Robert Bowman, Enid Sullivan, Soondong Kwon, Elaine Darby, Li-Jung Chen, and Craig Altare. Long Term Field Development of a Surfactant Modified Zeolite/Vapor Phase Bioreactor System for Treatment of Produced Waters for Power Generation. Office of Scientific and Technical Information (OSTI), December 2007. http://dx.doi.org/10.2172/962927.
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Hoover, Natasha L., and Michelle L. Soupir. Experimental Tile Drainage Denitrification Bioreactors: Pilot-Scale System for Replicated Field Research. Ames: Iowa State University, Digital Repository, 2017. http://dx.doi.org/10.31274/farmprogressreports-180814-1738.
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Field demonstration of vapor phase TCE bioreactor. Final report. Office of Scientific and Technical Information (OSTI), December 1994. http://dx.doi.org/10.2172/110798.